MD07G1307300.v1.1

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0001114
Physical Location & Seq
Forward (+)
36346901 .. 36347588
688 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1307300.v1.1.491

Sequence Viewer

Length: 486 bp
ATGGTATCTTTTTATCTCCTCTCCTCATATGCCGTGAGGAGTTGCCCCTCCTCTCTGAAGCTCTTGGGCTACTGTTTAGAGACTGAAGCACCCATGTTGGTCTATGTACTCGCCCGCAACGGGACCTTGTTCGACTACATTCATCATGTTAATGGGAAAAAAGCATTGTCTTGGCATATTCTTTTAAAGATAGCCTCAGAATCTGCAGCTGCTCTTGCTTATCTGCATTCTGCAGCAGAGACATCAAAACCAATGATCATTCATGGAAATGTCAACTCTTCCAACATATTATTAAGCGATTGTTTTGTGGCCAAGGTCTTCGGTTTCGGATCTTCAAGGTTGGTCCGGGTCCCTAGTAATGAATCCCAGATGAACACATTGGTACAGCAGACGGTTGGTTATCTAGACCCCGAATATCTCCAAACAGGCCAGTTGACAGATAGAGTGATGTCTATAGCTTTGGAGTCGTCTTGTTATAACTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000902 GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0005975 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006884 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007166 GO:0008150 GO:0008152 GO:0008361 GO:0009311 GO:0009505 GO:0009605 GO:0009607 GO:0009615 GO:0009620 GO:0009653 GO:0009719 GO:0009725 GO:0009751 GO:0009826 GO:0009987 GO:0009992 GO:0010033 GO:0014070 GO:0016020 GO:0016043 GO:0016049 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019725 GO:0023052 GO:0030104 GO:0030312 GO:0032502 GO:0032535 GO:0032989 GO:0036211 GO:0040007 GO:0042221 GO:0042493 GO:0042592 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046677 GO:0048589 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055082 GO:0060560 GO:0065007 GO:0065008 GO:0071704 GO:0071840 GO:0071944 GO:0090066 GO:0098542 GO:0140096 GO:1901564 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr

Protein Analysis

162

Amino Acids

17.75

Weight (kDa)

6.95

Isoelectric Point (pI)

48.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 16 - 147 1.1e-13 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 19 - 146 2.5e-10 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 477
AciI CCGC 1 cut(s) 115
AclWI GGATC 1 cut(s) 337
AcoI YGGCCR 1 cut(s) 309
AcuI CTGAAG 2 cut(s) 77, 105
AfaI GTAC 2 cut(s) 108, 384
AfiI CCNNNNNNNGG 1 cut(s) 120
AgsI TTSAA 1 cut(s) 336
AluBI AGCT 3 cut(s) 61, 209, 458
AluI AGCT 3 cut(s) 61, 209, 458
Alw26I GTCTC 2 cut(s) 74, 233
AlwI GGATC 1 cut(s) 337
AlwNI CAGNNNCTG 1 cut(s) 203
AoxI GGCC 2 cut(s) 309, 427
ApeKI GCWGC 3 cut(s) 206, 209, 233
AspS9I GGNCC 3 cut(s) 123, 343, 349
AsuC2I CCSGG 1 cut(s) 347
AvaII GGWCC 3 cut(s) 123, 343, 349
BalI TGGCCA 1 cut(s) 311
BbsI GAAGAC 1 cut(s) 310
BbvI GCAGC 3 cut(s) 196, 218, 245
BceAI ACGGC 1 cut(s) 17
BclI TGATCA 1 cut(s) 255
BcnI CCSGG 1 cut(s) 347
BcoDI GTCTC 2 cut(s) 74, 233
BfaI CTAG 2 cut(s) 354, 404
BfmI CTRYAG 3 cut(s) 204, 231, 453
BisI GCNGC 3 cut(s) 207, 210, 234
BlsI GCNGC 3 cut(s) 208, 211, 235
Bme1390I CCNGG 1 cut(s) 347
Bme18I GGWCC 3 cut(s) 123, 343, 349
BmgT120I GGNCC 3 cut(s) 123, 343, 349
BmiI GGNNCC 3 cut(s) 124, 350, 351
BmrFI CCNGG 1 cut(s) 347
BpiI GAAGAC 1 cut(s) 310
BpuMI CCSGG 1 cut(s) 347
BsaJI CCNNGG 1 cut(s) 312
Bsc4I CCNNNNNNNGG 1 cut(s) 120
Bse1I ACTGG 1 cut(s) 430
BseDI CCNNGG 1 cut(s) 312
BseLI CCNNNNNNNGG 1 cut(s) 120
BseMII CTCAG 1 cut(s) 210
BseNI ACTGG 1 cut(s) 430
BseRI GAGGAG 4 cut(s) 8, 13, 40, 52
BseXI GCAGC 3 cut(s) 196, 218, 245
BshFI GGCC 2 cut(s) 311, 429
BsiSI CCGG 1 cut(s) 346
BslFI GGGAC 2 cut(s) 136, 335
BslI CCNNNNNNNGG 1 cut(s) 120
BsmAI GTCTC 2 cut(s) 74, 233
BsmFI GGGAC 2 cut(s) 136, 335
BsmI GAATGC 1 cut(s) 226
BsnI GGCC 2 cut(s) 311, 429
Bsp143I GATC 2 cut(s) 255, 329
BspACI CCGC 1 cut(s) 115
BspANI GGCC 2 cut(s) 311, 429
BspCNI CTCAG 1 cut(s) 209
BspLI GGNNCC 3 cut(s) 124, 350, 351
BspMAI CTGCAG 2 cut(s) 208, 235
BspPI GGATC 1 cut(s) 337
BsrI ACTGG 1 cut(s) 430
BssECI CCNNGG 1 cut(s) 312
BssMI GATC 2 cut(s) 255, 329
BssT1I CCWWGG 1 cut(s) 312
Bst4CI ACNGT 2 cut(s) 74, 394
Bst6I CTCTTC 1 cut(s) 283
BstC8I GCNNGC 1 cut(s) 115
BstDEI CTNAG 1 cut(s) 196
BstKTI GATC 2 cut(s) 258, 332
BstMAI GTCTC 2 cut(s) 74, 233
BstMBI GATC 2 cut(s) 255, 329
BstMWI GCNNNNNNNGC 1 cut(s) 215
BstSCI CCNGG 1 cut(s) 345
BstSFI CTRYAG 3 cut(s) 204, 231, 453
BstV1I GCAGC 3 cut(s) 196, 218, 245
BstV2I GAAGAC 1 cut(s) 310
BstX2I RGATCY 1 cut(s) 329
BstYI RGATCY 1 cut(s) 329
BsuRI GGCC 2 cut(s) 311, 429
Cac8I GCNNGC 1 cut(s) 115
CaiI CAGNNNCTG 1 cut(s) 203
Cfr13I GGNCC 3 cut(s) 123, 343, 349
Csp6I GTAC 2 cut(s) 107, 383
CviAII CATG 3 cut(s) 94, 146, 263
CviJI RGCY 7 cut(s) 61, 69, 194, 209, 311, 429, 458
CviKI_1 RGCY 7 cut(s) 61, 69, 194, 209, 311, 429, 458
CviQI GTAC 2 cut(s) 107, 383
DdeI CTNAG 1 cut(s) 196
DpnI GATC 2 cut(s) 257, 331
DpnII GATC 2 cut(s) 255, 329
DraI TTTAAA 1 cut(s) 186
EaeI YGGCCR 1 cut(s) 309
Eam1104I CTCTTC 1 cut(s) 283
EarI CTCTTC 1 cut(s) 283
Eco130I CCWWGG 1 cut(s) 312
Eco47I GGWCC 3 cut(s) 123, 343, 349
Eco57I CTGAAG 2 cut(s) 77, 105
EcoO109I RGGNCCY 2 cut(s) 123, 349
EcoT14I CCWWGG 1 cut(s) 312
ErhI CCWWGG 1 cut(s) 312
FaeI CATG 3 cut(s) 97, 149, 266
FaqI GGGAC 2 cut(s) 136, 335
FatI CATG 3 cut(s) 93, 145, 262
FauI CCCGC 1 cut(s) 122
FauNDI CATATG 1 cut(s) 28
FbaI TGATCA 1 cut(s) 255
Fnu4HI GCNGC 3 cut(s) 207, 210, 234
Fsp4HI GCNGC 3 cut(s) 207, 210, 234
FspBI CTAG 2 cut(s) 354, 404
GluI GCNGC 3 cut(s) 207, 210, 234
HaeIII GGCC 2 cut(s) 311, 429
HapII CCGG 1 cut(s) 346
Hin1II CATG 3 cut(s) 97, 149, 266
HincII GTYRAC 2 cut(s) 274, 435
HindII GTYRAC 2 cut(s) 274, 435
HinfI GANTC 3 cut(s) 200, 362, 464
HpaII CCGG 1 cut(s) 346
Hpy166II GTNNAC 2 cut(s) 274, 435
Hpy188I TCNGA 3 cut(s) 57, 199, 329
Hpy188III TCNNGA 1 cut(s) 404
Hpy8I GTNNAC 2 cut(s) 274, 435
HpyCH4III ACNGT 2 cut(s) 74, 394
HpyCH4V TGCA 3 cut(s) 206, 226, 233
HpyF10VI GCNNNNNNNGC 1 cut(s) 215
HpyF3I CTNAG 1 cut(s) 196
Hsp92II CATG 3 cut(s) 97, 149, 266
KflI GGGWCCC 1 cut(s) 349
Ksp22I TGATCA 1 cut(s) 255
Kzo9I GATC 2 cut(s) 255, 329
LpnPI CCDG 4 cut(s) 359, 380, 411, 443
Lsp1109I GCAGC 3 cut(s) 196, 218, 245
MaeI CTAG 2 cut(s) 354, 404
MalI GATC 2 cut(s) 257, 331
MboI GATC 2 cut(s) 255, 329
MboII GAAGA 3 cut(s) 270, 310, 324
MflI RGATCY 1 cut(s) 329
MlsI TGGCCA 1 cut(s) 311
MluNI TGGCCA 1 cut(s) 311
MlyI GAGTC 1 cut(s) 473
MmeI TCCRAC 1 cut(s) 306
MnlI CCTC 6 cut(s) 29, 30, 34, 58, 61, 205
Mox20I TGGCCA 1 cut(s) 311
MscI TGGCCA 1 cut(s) 311
MseI TTAA 3 cut(s) 150, 185, 293
MslI CAYNNNNRTG 2 cut(s) 150, 267
Msp20I TGGCCA 1 cut(s) 311
MspA1I CMGCKG 1 cut(s) 209
MspI CCGG 1 cut(s) 346
MspR9I CCNGG 1 cut(s) 347
Mva1269I GAATGC 1 cut(s) 226
MwoI GCNNNNNNNGC 1 cut(s) 215
NciI CCSGG 1 cut(s) 347
NdeI CATATG 1 cut(s) 28
NdeII GATC 2 cut(s) 255, 329
NlaIII CATG 3 cut(s) 97, 149, 266
NlaIV GGNNCC 3 cut(s) 124, 350, 351
PctI GAATGC 1 cut(s) 226
PfeI GAWTC 2 cut(s) 200, 362
PkrI GCNGC 3 cut(s) 208, 211, 235
PleI GAGTC 1 cut(s) 472
PpsI GAGTC 1 cut(s) 472
PpuMI RGGWCCY 2 cut(s) 123, 349
PsiI TTATAA 1 cut(s) 477
Psp5II RGGWCCY 2 cut(s) 123, 349
PspN4I GGNNCC 3 cut(s) 124, 350, 351
PspPI GGNCC 3 cut(s) 123, 343, 349
PspPPI RGGWCCY 2 cut(s) 123, 349
PstI CTGCAG 2 cut(s) 208, 235
PstNI CAGNNNCTG 1 cut(s) 203
PsuI RGATCY 1 cut(s) 329
PvuII CAGCTG 1 cut(s) 209
RsaI GTAC 2 cut(s) 108, 384
RsaNI GTAC 2 cut(s) 107, 383
RseI CAYNNNNRTG 2 cut(s) 150, 267
SaqAI TTAA 3 cut(s) 150, 185, 293
SatI GCNGC 3 cut(s) 207, 210, 234
Sau3AI GATC 2 cut(s) 255, 329
Sau96I GGNCC 3 cut(s) 123, 343, 349
SchI GAGTC 1 cut(s) 473
ScrFI CCNGG 1 cut(s) 347
SetI ASST 6 cut(s) 63, 128, 211, 318, 341, 460
SfcI CTRYAG 3 cut(s) 204, 231, 453
SinI GGWCC 3 cut(s) 123, 343, 349
SmiMI CAYNNNNRTG 2 cut(s) 150, 267
SsiI CCGC 1 cut(s) 115
SspMI CTAG 2 cut(s) 354, 404
StyD4I CCNGG 1 cut(s) 345
StyI CCWWGG 1 cut(s) 312
TaaI ACNGT 2 cut(s) 74, 394
TaqI TCGA 1 cut(s) 132
TatI WGTACW 1 cut(s) 106
TfiI GAWTC 2 cut(s) 200, 362
Tru1I TTAA 3 cut(s) 150, 185, 293
Tru9I TTAA 3 cut(s) 150, 185, 293
TseI GCWGC 3 cut(s) 206, 209, 233
TspDTI ATGAA 4 cut(s) 131, 251, 375, 386
VpaK11BI GGWCC 3 cut(s) 123, 343, 349
XbaI TCTAGA 1 cut(s) 403
XspI CTAG 2 cut(s) 354, 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.