FvH4_2g04620

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000864
Physical Location & Seq
Reverse (-)
3537928 .. 3540019
2092 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g04620.t1

Sequence Viewer

Length: 1773 bp
ATGGACTATGAAAGTGATGACGGCTATGGATCCGCAGAGAGTGGCAATGACGAAGCATTGAACTACGATGACGATCTTCAACTGGATGAAGATGATAAGGTCGATGAGTATGGTGAGAGTAGGTGTTCACTGGAAAATTATACTGTTTTGAAAGAAGAAGAAATTGCAAAACTTCAGGATAGTTGTACCGCAGATGTCTCTACTGTTCTCTCCATATCCAAGTCCGATGCGTGCTTACTACTCCCTCGCTTTCATTGGAGGGTTGAAGAACTTAAGGATGAATGGTTTGCCGATGAAGATAAAGTTAGAGAAGATGCAGGTTTGTTGAAGAAACCTATAGTGGAACATAATGCTAGGGCAAATCTAAGAATTGCTTGCGGAATTTGTTTTGAAGAGTACAACTATGATCGGTTTATTTTATCTGCCTCTTGTGGCCATCCTTTTTGTCGAGATTGCTGGGCAGGTTATATTAGCACAACAATCTGTGATGGTGGTCCGGCTTGTGTGAGGCTAAGGTGTCCTGAACCATCATGTAATGCAGCAGTTGGTCCTGATTTGATCGAAAAGGTGTTGTTGAACTCAAACCGAGGAGCTGAGCATGGCAAGTACAAGACATATCTGTTAAGATCTTACGTCGAAGATCAGAAGAACATCAAGTGGTGTCCTTCCCCGGATTGTAACTACGCAATTCAGTTTCATGCTTTGGGTGGATTAATGGTGTCATATGATGTTTCGTGTCTTTGCTCGCATAGATATTGTTGGAATTGTATTGAAGAGAATCATTGTCCGGTGGATTGTGAAACTGTGGGGAAGTGGATATTGAAGAACAAGGATGACTCTCAAAATACACAATGGATACTGGTTAATTCCAAGCCTTGTCCCAAATGCAAAAGACCAATTGAGAAGAACCAAGGCTGTAATCATATGACTTGCAGTCCTCCCTGTGGGTACCAGTTTTGTTGGTTATGCCTCGGGCCAAATAAAAGTTACTCTGGTTGCTATTGCAATAGGTTTAGGCAGGGGTCAATTGATAATCCTGAGACGTCTATGAATACGGATGAGAAGGCAAGGAAGAAGGCAAAGATGTACTTAGAGAGATACATTCATTATTATGAAAGGTGGGAAACCAATGGAAGATCAAAGAAAAAAGCTCTGGAGGATTTTAATAAGGTGAAGACCGAGCACATTAAGCAACTTGGGGAGATACAAGGTAACACAAGAGAGCATGAATACGATTTTATCATAGAGGCCTGGCAACAGATTATTGACTGCAGGCAAGTATTGCGATGGACCTATGCGTATGGCTACTACCTGCCTGAGGATACTGCCGCTAAGAAGCACCTTTTCGAGTGTTTGCAAGGCTATGCTGAGTTCAGTCTCGAAAGGCTTCACAACTGCGCCGAGAGTGAACTACAACCGCTTCTGGTCTCATCATCATCGGCAGCTGATGAAGATTTCAAAAAGTTCAAGACAAAACTCATGGGGCTGACAAAGGTGACCGGAACTTATTTCAAAAAGTTGGTTACTGCGTTGGAGGAGGGGCTTCCGGAGACGCTTCAATATGGAGCTGGAGCTGCTTTCAAGGAGATTGACTGGGCATGTGACCAATGTACCTATATCAATGTAGGGTCTGCCGTTAACTGTGAAGTATGTTTTGAGGAAAATCCAAAAGGTTGCTGGTCTTGTGAGCGTTGTACACTTTCGAATCCGGCGACTAGAACCAGGTGTCTGGTGTGTGATAATACCGAAGATGTTGATCAGGGTGTAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IBR zf-RanBP

Protein Analysis

591

Amino Acids

67.39

Weight (kDa)

5.08

Isoelectric Point (pI)

50.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UBA_ARI1 PF21235 58 - 96 1.1e-07 E3 ubiquitin-protein ligase ARIH1, UBA-like domain
IBR PF01485 202 - 266 1.3e-13 IBR domain, a half RING-finger domain
IBR_2 PF22605 286 - 325 1.7e-10 IBR domain
IBR_1 PF22191 289 - 326 2.9e-10 IBR domain
Rcat_RNF216 PF26200 291 - 342 7.7e-07 RNF216 Rcat domain
Ariadne PF19422 365 - 522 1.2e-07 Ariadne domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1046
Acc36I ACCTGC 3 cut(s) 308, 452, 1320
Acc65I GGTACC 1 cut(s) 948
AccB1I GGYRCC 1 cut(s) 948
AccIII TCCGGA 1 cut(s) 1546
AciI CCGC 5 cut(s) 33, 189, 378, 1329, 1418
AclWI GGATC 2 cut(s) 24, 37
AcoI YGGCCR 1 cut(s) 433
AcsI RAATTY 1 cut(s) 381
AcuI CTGAAG 1 cut(s) 158
AcyI GRCGYC 1 cut(s) 1043
AfaI GTAC 7 cut(s) 187, 398, 608, 950, 1088, 1612, 1696
AfiI CCNNNNNNNGG 2 cut(s) 944, 1318
AflII CTTAAG 1 cut(s) 272
AhdI GACNNNNNGTC 1 cut(s) 933
AjnI CCWGG 2 cut(s) 1250, 1721
AluBI AGCT 5 cut(s) 593, 1151, 1445, 1568, 1574
AluI AGCT 5 cut(s) 593, 1151, 1445, 1568, 1574
Alw21I GWGCWC 1 cut(s) 1185
Alw26I GTCTC 5 cut(s) 202, 1034, 1382, 1432, 1544
AlwI GGATC 2 cut(s) 24, 37
Ama87I CYCGRG 1 cut(s) 971
Aor13HI TCCGGA 1 cut(s) 1546
AoxI GGCC 3 cut(s) 433, 974, 1248
ApeKI GCWGC 3 cut(s) 539, 1442, 1574
ApoI RAATTY 1 cut(s) 381
AseI ATTAAT 1 cut(s) 713
Asp700I GAANNNNTTC 1 cut(s) 1386
Asp718I GGTACC 1 cut(s) 948
AspLEI GCGC 1 cut(s) 1400
AspS9I GGNCC 4 cut(s) 494, 548, 974, 1290
AsuC2I CCSGG 1 cut(s) 671
AsuHPI GGTGA 3 cut(s) 125, 1183, 1507
AsuII TTCGAA 1 cut(s) 1703
AvaI CYCGRG 1 cut(s) 971
AvaII GGWCC 3 cut(s) 494, 548, 1290
AxyI CCTNAGG 1 cut(s) 1317
BaeI ACNNNNGTAYC 2 cut(s) 1594, 1627
BalI TGGCCA 1 cut(s) 435
BamHI GGATCC 1 cut(s) 29
BanI GGYRCC 1 cut(s) 948
BbsI GAAGAC 1 cut(s) 1181
Bbv12I GWGCWC 1 cut(s) 1185
BbvI GCAGC 3 cut(s) 551, 1454, 1561
BccI CCATC 4 cut(s) 444, 482, 535, 1281
BceAI ACGGC 2 cut(s) 37, 1619
BciT130I CCWGG 2 cut(s) 1252, 1723
BciVI GTATCC 2 cut(s) 849, 1315
BclI TGATCA 1 cut(s) 1756
BcnI CCSGG 1 cut(s) 671
BcoDI GTCTC 5 cut(s) 202, 1034, 1382, 1432, 1544
BfaI CTAG 2 cut(s) 354, 1716
BfmI CTRYAG 2 cut(s) 336, 1270
BfrI CTTAAG 1 cut(s) 272
BfuAI ACCTGC 3 cut(s) 308, 452, 1320
BfuI GTATCC 2 cut(s) 849, 1315
BglII AGATCT 1 cut(s) 626
BisI GCNGC 4 cut(s) 540, 1329, 1443, 1575
BlpI GCTNAGC 1 cut(s) 594
BlsI GCNGC 4 cut(s) 541, 1330, 1444, 1576
Bme1390I CCNGG 3 cut(s) 671, 1252, 1723
Bme18I GGWCC 3 cut(s) 494, 548, 1290
BmeRI GACNNNNNGTC 1 cut(s) 933
BmeT110I CYCGRG 1 cut(s) 971
BmgT120I GGNCC 4 cut(s) 494, 548, 974, 1290
BmiI GGNNCC 2 cut(s) 31, 950
BmrFI CCNGG 3 cut(s) 671, 1252, 1723
BmrI ACTGGG 1 cut(s) 1603
BmsI GCATC 2 cut(s) 217, 304
BmuI ACTGGG 1 cut(s) 1603
BpiI GAAGAC 1 cut(s) 1181
BpmI CTGGAG 2 cut(s) 1175, 1590
Bpu10I CCTNAGC 1 cut(s) 512
Bpu1102I GCTNAGC 1 cut(s) 594
Bpu14I TTCGAA 1 cut(s) 1703
BpuMI CCSGG 1 cut(s) 671
BsaBI GATNNNNATC 2 cut(s) 72, 1755
BsaHI GRCGYC 1 cut(s) 1043
BsaI GGTCTC 1 cut(s) 1432
BsaJI CCNNGG 4 cut(s) 586, 669, 910, 970
BsaWI WCCGGW 3 cut(s) 787, 1499, 1546
Bsc4I CCNNNNNNNGG 2 cut(s) 944, 1318
Bse1I ACTGG 5 cut(s) 87, 135, 864, 952, 1598
Bse21I CCTNAGG 1 cut(s) 1317
Bse3DI GCAATG 1 cut(s) 52
Bse8I GATNNNNATC 2 cut(s) 72, 1755
BseAI TCCGGA 1 cut(s) 1546
BseBI CCWGG 2 cut(s) 1252, 1723
BseDI CCNNGG 4 cut(s) 586, 669, 910, 970
BseGI GGATG 5 cut(s) 91, 283, 436, 838, 1063
BseJI GATNNNNATC 2 cut(s) 72, 1755
BseLI CCNNNNNNNGG 2 cut(s) 944, 1318
BseMI GCAATG 1 cut(s) 52
BseMII CTCAG 4 cut(s) 585, 1029, 1308, 1359
BseNI ACTGG 5 cut(s) 87, 135, 864, 952, 1598
BseRI GAGGAG 2 cut(s) 603, 1550
BseXI GCAGC 3 cut(s) 551, 1454, 1561
BseYI CCCAGC 1 cut(s) 456
BshFI GGCC 3 cut(s) 435, 976, 1250
BshNI GGYRCC 1 cut(s) 948
BsiHKAI GWGCWC 1 cut(s) 1185
BsiHKCI CYCGRG 1 cut(s) 971
BsiSI CCGG 6 cut(s) 497, 671, 788, 1500, 1547, 1709
BslFI GGGAC 1 cut(s) 864
BslI CCNNNNNNNGG 2 cut(s) 944, 1318
BsmAI GTCTC 5 cut(s) 202, 1034, 1382, 1432, 1544
BsmBI CGTCTC 2 cut(s) 1034, 1544
BsmFI GGGAC 1 cut(s) 864
BsnI GGCC 3 cut(s) 435, 976, 1250
Bso31I GGTCTC 1 cut(s) 1432
BsoBI CYCGRG 1 cut(s) 971
Bsp119I TTCGAA 1 cut(s) 1703
Bsp1286I GDGCHC 1 cut(s) 1185
Bsp13I TCCGGA 1 cut(s) 1546
Bsp1407I TGTACA 1 cut(s) 1694
Bsp143I GATC 8 cut(s) 29, 73, 406, 558, 626, 640, 1136, 1756
Bsp1720I GCTNAGC 1 cut(s) 594
BspACI CCGC 5 cut(s) 33, 189, 378, 1329, 1418
BspANI GGCC 3 cut(s) 435, 976, 1250
BspCNI CTCAG 4 cut(s) 586, 1030, 1309, 1360
BspEI TCCGGA 1 cut(s) 1546
BspLI GGNNCC 2 cut(s) 31, 950
BspMAI CTGCAG 1 cut(s) 1274
BspMI ACCTGC 3 cut(s) 308, 452, 1320
BspPI GGATC 2 cut(s) 24, 37
BspT104I TTCGAA 1 cut(s) 1703
BspT107I GGYRCC 1 cut(s) 948
BspTI CTTAAG 1 cut(s) 272
BspTNI GGTCTC 1 cut(s) 1432
BsrDI GCAATG 1 cut(s) 52
BsrGI TGTACA 1 cut(s) 1694
BsrI ACTGG 5 cut(s) 87, 135, 864, 952, 1598
BssECI CCNNGG 4 cut(s) 586, 669, 910, 970
BssMI GATC 8 cut(s) 29, 73, 406, 558, 626, 640, 1136, 1756
BssNI GRCGYC 1 cut(s) 1043
BssT1I CCWWGG 1 cut(s) 910
Bst2UI CCWGG 2 cut(s) 1252, 1723
Bst4CI ACNGT 4 cut(s) 145, 205, 805, 1643
Bst6I CTCTTC 2 cut(s) 387, 768
BstACI GRCGYC 1 cut(s) 1043
BstAFI CTTAAG 1 cut(s) 272
BstAPI GCANNNNNTGC 1 cut(s) 1282
BstAUI TGTACA 1 cut(s) 1694
BstBI TTCGAA 1 cut(s) 1703
BstC8I GCNNGC 4 cut(s) 232, 376, 746, 1274
BstDEI CTNAG 8 cut(s) 365, 512, 594, 1038, 1090, 1317, 1332, 1368
BstEII GGTNACC 1 cut(s) 1495
BstENI CCTNNNNNAGG 1 cut(s) 1316
BstF5I GGATG 5 cut(s) 91, 283, 436, 838, 1063
BstHHI GCGC 1 cut(s) 1400
BstKTI GATC 8 cut(s) 32, 76, 409, 561, 629, 643, 1139, 1759
BstMAI GTCTC 5 cut(s) 202, 1034, 1382, 1432, 1544
BstMBI GATC 8 cut(s) 29, 73, 406, 558, 626, 640, 1136, 1756
BstMWI GCNNNNNNNGC 3 cut(s) 1189, 1282, 1574
BstNI CCWGG 2 cut(s) 1252, 1723
BstNSI RCATGY 1 cut(s) 1602
BstPI GGTNACC 1 cut(s) 1495
BstSCI CCNGG 3 cut(s) 669, 1250, 1721
BstSFI CTRYAG 2 cut(s) 336, 1270
BstV1I GCAGC 3 cut(s) 551, 1454, 1561
BstV2I GAAGAC 1 cut(s) 1181
BstX2I RGATCY 2 cut(s) 29, 626
BstXI CCANNNNNNTGG 1 cut(s) 1729
BstYI RGATCY 2 cut(s) 29, 626
Bsu36I CCTNAGG 1 cut(s) 1317
BsuI GTATCC 2 cut(s) 849, 1315
BsuRI GGCC 3 cut(s) 435, 976, 1250
BtgZI GCGATG 1 cut(s) 1300
BtsCI GGATG 5 cut(s) 91, 283, 436, 838, 1063
BtsIMutI CAGTG 1 cut(s) 128
BveI ACCTGC 3 cut(s) 308, 452, 1320
Cac8I GCNNGC 4 cut(s) 232, 376, 746, 1274
CfoI GCGC 1 cut(s) 1400
Cfr13I GGNCC 4 cut(s) 494, 548, 974, 1290
CseI GACGC 1 cut(s) 1561
CsiI ACCWGGT 1 cut(s) 1721
Csp6I GTAC 7 cut(s) 186, 397, 607, 949, 1087, 1611, 1695
CviAII CATG 6 cut(s) 531, 599, 698, 1226, 1480, 1599
CviQI GTAC 7 cut(s) 186, 397, 607, 949, 1087, 1611, 1695
DdeI CTNAG 8 cut(s) 365, 512, 594, 1038, 1090, 1317, 1332, 1368
DpnI GATC 8 cut(s) 31, 75, 408, 560, 628, 642, 1138, 1758
DpnII GATC 8 cut(s) 29, 73, 406, 558, 626, 640, 1136, 1756
DriI GACNNNNNGTC 1 cut(s) 933
EaeI YGGCCR 1 cut(s) 433
Eam1104I CTCTTC 2 cut(s) 387, 768
Eam1105I GACNNNNNGTC 1 cut(s) 933
EarI CTCTTC 2 cut(s) 387, 768
Eco130I CCWWGG 1 cut(s) 910
Eco147I AGGCCT 1 cut(s) 1250
Eco31I GGTCTC 1 cut(s) 1432
Eco47I GGWCC 3 cut(s) 494, 548, 1290
Eco57I CTGAAG 1 cut(s) 158
Eco81I CCTNAGG 1 cut(s) 1317
Eco88I CYCGRG 1 cut(s) 971
Eco91I GGTNACC 1 cut(s) 1495
EcoNI CCTNNNNNAGG 1 cut(s) 1316
EcoO65I GGTNACC 1 cut(s) 1495
EcoRII CCWGG 2 cut(s) 1250, 1721
EcoT14I CCWWGG 1 cut(s) 910
ErhI CCWWGG 1 cut(s) 910
Esp3I CGTCTC 2 cut(s) 1034, 1544
FaeI CATG 6 cut(s) 534, 602, 701, 1229, 1483, 1602
FalI AAGNNNNNCTT 4 cut(s) 358, 390, 1073, 1105
FaqI GGGAC 1 cut(s) 864
FatI CATG 6 cut(s) 530, 598, 697, 1225, 1479, 1598
FauNDI CATATG 2 cut(s) 724, 924
FbaI TGATCA 1 cut(s) 1756
Fnu4HI GCNGC 4 cut(s) 540, 1329, 1443, 1575
FokI GGATG 5 cut(s) 98, 290, 423, 845, 1070
Fsp4HI GCNGC 4 cut(s) 540, 1329, 1443, 1575
FspBI CTAG 2 cut(s) 354, 1716
GlaI GCGC 1 cut(s) 1399
GluI GCNGC 4 cut(s) 540, 1329, 1443, 1575
GsaI CCCAGC 1 cut(s) 460
GsuI CTGGAG 2 cut(s) 1175, 1590
HaeIII GGCC 3 cut(s) 435, 976, 1250
HapII CCGG 6 cut(s) 497, 671, 788, 1500, 1547, 1709
HgaI GACGC 1 cut(s) 1561
HhaI GCGC 1 cut(s) 1400
Hin1I GRCGYC 1 cut(s) 1043
Hin1II CATG 6 cut(s) 534, 602, 701, 1229, 1483, 1602
Hin6I GCGC 1 cut(s) 1398
HinP1I GCGC 1 cut(s) 1398
HincII GTYRAC 1 cut(s) 1639
HindII GTYRAC 1 cut(s) 1639
HinfI GANTC 3 cut(s) 778, 836, 1705
HpaI GTTAAC 1 cut(s) 1639
HpaII CCGG 6 cut(s) 497, 671, 788, 1500, 1547, 1709
HphI GGTGA 3 cut(s) 125, 1183, 1507
Hpy166II GTNNAC 4 cut(s) 128, 1409, 1639, 1697
Hpy188I TCNGA 2 cut(s) 226, 645
Hpy188III TCNNGA 9 cut(s) 176, 449, 521, 551, 1037, 1154, 1379, 1468, 1547
Hpy8I GTNNAC 4 cut(s) 128, 1409, 1639, 1697
Hpy99I CGWCG 1 cut(s) 638
HpyAV CCTTC 3 cut(s) 675, 1057, 1069
HpyCH4III ACNGT 4 cut(s) 145, 205, 805, 1643
HpyCH4IV ACGT 2 cut(s) 633, 1043
HpyCH4V TGCA 8 cut(s) 167, 317, 539, 888, 933, 1005, 1272, 1357
HpyF10VI GCNNNNNNNGC 3 cut(s) 1189, 1282, 1574
HpyF3I CTNAG 8 cut(s) 365, 512, 594, 1038, 1090, 1317, 1332, 1368
HpySE526I ACGT 2 cut(s) 633, 1043
Hsp92I GRCGYC 1 cut(s) 1043
Hsp92II CATG 6 cut(s) 534, 602, 701, 1229, 1483, 1602
HspAI GCGC 1 cut(s) 1398
Kpn2I TCCGGA 1 cut(s) 1546
KpnI GGTACC 1 cut(s) 952
Ksp22I TGATCA 1 cut(s) 1756
KspAI GTTAAC 1 cut(s) 1639
Kzo9I GATC 8 cut(s) 29, 73, 406, 558, 626, 640, 1136, 1756
LmnI GCTCC 3 cut(s) 590, 1565, 1571
Lsp1109I GCAGC 3 cut(s) 551, 1454, 1561
LweI GCATC 2 cut(s) 217, 304
MabI ACCWGGT 1 cut(s) 1721
MaeI CTAG 2 cut(s) 354, 1716
MaeII ACGT 2 cut(s) 633, 1043
MaeIII GTNAC 6 cut(s) 677, 986, 1211, 1495, 1522, 1601
MalI GATC 8 cut(s) 31, 75, 408, 560, 628, 642, 1138, 1758
MboI GATC 8 cut(s) 29, 73, 406, 558, 626, 640, 1136, 1756
MfeI CAATTG 2 cut(s) 897, 1026
MflI RGATCY 2 cut(s) 29, 626
MhlI GDGCHC 1 cut(s) 1185
MlsI TGGCCA 1 cut(s) 435
MluCI AATT 9 cut(s) 136, 162, 369, 381, 687, 763, 865, 897, 1026
MluNI TGGCCA 1 cut(s) 435
MlyI GAGTC 1 cut(s) 830
MmeI TCCRAC 2 cut(s) 740, 1512
Mox20I TGGCCA 1 cut(s) 435
MroI TCCGGA 1 cut(s) 1546
MroXI GAANNNNTTC 1 cut(s) 1386
MscI TGGCCA 1 cut(s) 435
MseI TTAA 7 cut(s) 273, 623, 713, 864, 1164, 1188, 1638
MslI CAYNNNNRTG 1 cut(s) 1110
Msp20I TGGCCA 1 cut(s) 435
MspA1I CMGCKG 1 cut(s) 1445
MspCI CTTAAG 1 cut(s) 272
MspI CCGG 6 cut(s) 497, 671, 788, 1500, 1547, 1709
MspR9I CCNGG 3 cut(s) 671, 1252, 1723
MunI CAATTG 2 cut(s) 897, 1026
MvaI CCWGG 2 cut(s) 1252, 1723
MwoI GCNNNNNNNGC 3 cut(s) 1189, 1282, 1574
NciI CCSGG 1 cut(s) 671
NdeI CATATG 2 cut(s) 724, 924
NdeII GATC 8 cut(s) 29, 73, 406, 558, 626, 640, 1136, 1756
NlaIII CATG 6 cut(s) 534, 602, 701, 1229, 1483, 1602
NlaIV GGNNCC 2 cut(s) 31, 950
NmeAIII GCCGAG 1 cut(s) 1426
NmuCI GTSAC 2 cut(s) 1495, 1601
NspI RCATGY 1 cut(s) 1602
NspV TTCGAA 1 cut(s) 1703
PceI AGGCCT 1 cut(s) 1250
PcsI WCGNNNNNNNCGW 1 cut(s) 1709
PdmI GAANNNNTTC 1 cut(s) 1386
PfeI GAWTC 2 cut(s) 778, 1705
PkrI GCNGC 4 cut(s) 541, 1330, 1444, 1576
PleI GAGTC 1 cut(s) 830
PpsI GAGTC 1 cut(s) 830
PshBI ATTAAT 1 cut(s) 713
Psp6I CCWGG 2 cut(s) 1250, 1721
PspEI GGTNACC 1 cut(s) 1495
PspFI CCCAGC 1 cut(s) 456
PspGI CCWGG 2 cut(s) 1250, 1721
PspN4I GGNNCC 2 cut(s) 31, 950
PspPI GGNCC 4 cut(s) 494, 548, 974, 1290
PstI CTGCAG 1 cut(s) 1274
PsuI RGATCY 2 cut(s) 29, 626
PvuII CAGCTG 1 cut(s) 1445
RsaI GTAC 7 cut(s) 187, 398, 608, 950, 1088, 1612, 1696
RsaNI GTAC 7 cut(s) 186, 397, 607, 949, 1087, 1611, 1695
RseI CAYNNNNRTG 1 cut(s) 1110
SaqAI TTAA 7 cut(s) 273, 623, 713, 864, 1164, 1188, 1638
SatI GCNGC 4 cut(s) 540, 1329, 1443, 1575
Sau3AI GATC 8 cut(s) 29, 73, 406, 558, 626, 640, 1136, 1756
Sau96I GGNCC 4 cut(s) 494, 548, 974, 1290
SchI GAGTC 1 cut(s) 830
ScrFI CCNGG 3 cut(s) 671, 1252, 1723
SduI GDGCHC 1 cut(s) 1185
SexAI ACCWGGT 1 cut(s) 1721
SfaNI GCATC 2 cut(s) 217, 304
SfcI CTRYAG 2 cut(s) 336, 1270
SfuI TTCGAA 1 cut(s) 1703
SinI GGWCC 3 cut(s) 494, 548, 1290
SmiMI CAYNNNNRTG 1 cut(s) 1110
SmlI CTYRAG 1 cut(s) 272
SmoI CTYRAG 1 cut(s) 272
Sse9I AATT 9 cut(s) 136, 162, 369, 381, 687, 763, 865, 897, 1026
SseBI AGGCCT 1 cut(s) 1250
SsiI CCGC 5 cut(s) 33, 189, 378, 1329, 1418
SspMI CTAG 2 cut(s) 354, 1716
StuI AGGCCT 1 cut(s) 1250
StyD4I CCNGG 3 cut(s) 669, 1250, 1721
StyI CCWWGG 1 cut(s) 910
TaaI ACNGT 4 cut(s) 145, 205, 805, 1643
TaiI ACGT 2 cut(s) 636, 1046
TaqI TCGA 7 cut(s) 102, 448, 561, 636, 1347, 1380, 1703
TaqII GACCGA 1 cut(s) 1193
TasI AATT 9 cut(s) 136, 162, 369, 381, 687, 763, 865, 897, 1026
TatI WGTACW 4 cut(s) 396, 606, 1086, 1694
TauI GCSGC 1 cut(s) 1331
TfiI GAWTC 2 cut(s) 778, 1705
Tru1I TTAA 7 cut(s) 273, 623, 713, 864, 1164, 1188, 1638
Tru9I TTAA 7 cut(s) 273, 623, 713, 864, 1164, 1188, 1638
TscAI CASTG 1 cut(s) 135
TseFI GTSAC 2 cut(s) 1495, 1601
TseI GCWGC 3 cut(s) 539, 1442, 1574
Tsp45I GTSAC 2 cut(s) 1495, 1601
TspGWI ACGGA 1 cut(s) 1070
TspRI CASTG 1 cut(s) 135
Vha464I CTTAAG 1 cut(s) 272
VpaK11BI GGWCC 3 cut(s) 494, 548, 1290
VspI ATTAAT 1 cut(s) 713
XagI CCTNNNNNAGG 1 cut(s) 1316
XapI RAATTY 1 cut(s) 381
XceI RCATGY 1 cut(s) 1602
XcmI CCANNNNNNNNNTGG 1 cut(s) 1674
XmnI GAANNNNTTC 1 cut(s) 1386
XspI CTAG 2 cut(s) 354, 1716
ZraI GACGTC 1 cut(s) 1044
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.