Rh6BG414600

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000864
Physical Location & Seq
Reverse (-)
64024780 .. 64026282
1503 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG414600.1

Sequence Viewer

Length: 1503 bp
ATGGATGACACAGACTACATGTACGATGACAACTACGACGACGACGGCTTTAGCGATTGTGACGACGATGATGATGATCAGTCGATTGAAGAGGAATCAAATGATCGGCAAAACTACACTGTTCTGAAAGAAGAAGAGATTGCCAAGCTTCAAGAGGATGCTATCACTGAAGTGTCTGCTGTACTATCAATATCGCAATCCGCTGCCTGCTTCGCCCTCACTCAGTTCAACTGGAACGTGATCAATCTCAACGACGAGTGGTTCACCGATGAAGACAGGATCCGGCAAAAGGTAGGTCTGTTGGAGAAGCCTGTGGCTCAATTCAATCCGAAAAACCAAAGAACTATTTGCGGTATATGTTTTGAGGTGTATGGCTATGGATCGATGTATTCGGCCGCTTGCGGTCATCGTTATTGTGGAGAGTGCTGGAGGGGTTACATCAGCACAGCGATCAAGGATGGCCCCCCGTGTTTGATGCTGAGGTGTCCCGAACCGCCGTGTAGGGCGGCGGTTGGTCCTGATTTGATCTCGGTCTTGGTGTCGAAACAGGAGTACGAGAGGTACAGGCACTACCTTTTGAGATCGTACGTCGAACAGCAGACCAAGATCAAGTGGTGTCCTGCTCCGGATTGTAAGTACGCTGTTAGGTTTGATAGTTATGAGACCAGAAGCTATGACATTTCGTGTCTTTGTTCGCATAGCTATTGCTGGAATTGTACGGAGGAGAGTCACCGTCCGGTGGACTGTGAGACGGTGAGGAAGTGGGTTATGAAGAACAAGGACAGCTCGCAAAATGCGCAGTGGATATTGGTTAACACCAAGCCTTGTCCAAAGTGCAAGAGACCGATTCAGAAGAACCAAGGGTGTCACCATATGACTTGCTCAGCTCCTTGTAGGTATGAGTTTTGCTGGTATTGTCTTGCGAAATTCGAAAGTTATTCTACTAGATGTTGTAACGCGTATAGGGCGAATACTGATGAGCTGAAGGAGTTGGATTTGGAGCCGGATCAGAAGAAGCTGAAGGAGTATTTAGATAGGTATCTTCATTATTATGAGCGATGGGCAAACAATCAGTCTTCGAAGGAAAAGGCTGTCGAGTCTTTAGAGGAGGTGCGAACCAAGCACATTATGGAGCTTATTAGGAAACAACAGTATCTGTCTGAGCTTGAACTCAATTTCATCATAGATGCATGGCAACAGATAATTGAATGTAGGCAGGTGCTGCGGTGGAGCTATGCGTATGGCTACTACATGCCTGAGGATGAGCGTCTTAAGGCTGATCTGTTTGAGCACTTGCAAGGTCATGCAGAGTACAGCCTCGAGAGGCTTCACAAATGCGCCGAGACTGAATTGCAACCATTTCTGGCTTCTGAGGAAGAATTAAAGGAGAGCTTCCATGCATTTCGCTTAAAACTCGAAAATATGACGAGGGCCACTAAAGATTATTTCAGGAAGCTGGTTACAGCCTTGGAGAATGGCCTTTCGGAGGTGGACGGCGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IBR zf-RanBP

Protein Analysis

500

Amino Acids

58.9

Weight (kDa)

5.05

Isoelectric Point (pI)

53.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UBA_ARI1 PF21235 50 - 88 3.6e-06 E3 ubiquitin-protein ligase ARIH1, UBA-like domain
IBR PF01485 186 - 249 9e-16 IBR domain, a half RING-finger domain
IBR_2 PF22605 269 - 311 6.2e-10 IBR domain
IBR_1 PF22191 272 - 311 2.1e-11 IBR domain
Ariadne PF19422 346 - 494 6.4e-07 Ariadne domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1207
Acc16I TGCGCA 1 cut(s) 798
Acc36I ACCTGC 1 cut(s) 1207
AccII CGCG 1 cut(s) 959
AccIII TCCGGA 1 cut(s) 625
AciI CCGC 8 cut(s) 201, 351, 396, 402, 494, 506, 509, 1225
AclWI GGATC 4 cut(s) 274, 287, 388, 1014
AcoI YGGCCR 1 cut(s) 393
AcsI RAATTY 1 cut(s) 926
AcuI CTGAAG 3 cut(s) 189, 1004, 1040
AfaI GTAC 8 cut(s) 23, 183, 554, 563, 587, 638, 718, 1313
AfiI CCNNNNNNNGG 3 cut(s) 289, 739, 1486
AflII CTTAAG 1 cut(s) 1271
AflIII ACRYGT 2 cut(s) 18, 957
AgsI TTSAA 6 cut(s) 89, 152, 229, 325, 1169, 1208
AleI CACNNNNGTG 1 cut(s) 170
Alw21I GWGCWC 1 cut(s) 1293
Alw26I GTCTC 4 cut(s) 656, 743, 835, 1337
AlwI GGATC 4 cut(s) 274, 287, 388, 1014
AlwNI CAGNNNCTG 2 cut(s) 1156, 1222
Ama87I CYCGRG 1 cut(s) 1319
Aor13HI TCCGGA 1 cut(s) 625
AoxI GGCC 4 cut(s) 393, 460, 1431, 1477
ApeKI GCWGC 2 cut(s) 203, 1222
ApoI RAATTY 1 cut(s) 926
ArsI GACNNNNNNTTYG 4 cut(s) 280, 312, 1058, 1090
AspLEI GCGC 2 cut(s) 799, 1340
AspS9I GGNCC 3 cut(s) 461, 515, 1431
AsuHPI GGTGA 4 cut(s) 256, 722, 766, 860
AsuII TTCGAA 2 cut(s) 930, 1079
AvaI CYCGRG 1 cut(s) 1319
AvaII GGWCC 1 cut(s) 515
AxyI CCTNAGG 1 cut(s) 1257
BaeI ACNNNNGTAYC 2 cut(s) 1136, 1169
BamHI GGATCC 1 cut(s) 279
BbsI GAAGAC 2 cut(s) 279, 1068
Bbv12I GWGCWC 1 cut(s) 1293
BbvCI CCTCAGC 1 cut(s) 479
BbvI GCAGC 2 cut(s) 190, 1209
BccI CCATC 2 cut(s) 452, 1053
BceAI ACGGC 2 cut(s) 61, 481
BclI TGATCA 2 cut(s) 76, 240
BcoDI GTCTC 4 cut(s) 656, 743, 835, 1337
BfaI CTAG 1 cut(s) 945
BfrI CTTAAG 1 cut(s) 1271
BfuAI ACCTGC 1 cut(s) 1207
BisI GCNGC 4 cut(s) 204, 396, 507, 1223
BlpI GCTNAGC 1 cut(s) 883
BlsI GCNGC 4 cut(s) 205, 397, 508, 1224
Bme18I GGWCC 1 cut(s) 515
BmeT110I CYCGRG 1 cut(s) 1319
BmgT120I GGNCC 3 cut(s) 461, 515, 1431
BmiI GGNNCC 3 cut(s) 281, 463, 1002
BmsI GCATC 3 cut(s) 148, 465, 1177
BpiI GAAGAC 2 cut(s) 279, 1068
BpmI CTGGAG 1 cut(s) 448
Bpu10I CCTNAGC 1 cut(s) 479
Bpu1102I GCTNAGC 1 cut(s) 883
Bpu14I TTCGAA 2 cut(s) 930, 1079
Bsa29I ATCGAT 1 cut(s) 383
BsaBI GATNNNNATC 2 cut(s) 75, 1038
BsaI GGTCTC 2 cut(s) 656, 835
BsaJI CCNNGG 2 cut(s) 859, 1467
BsaWI WCCGGW 2 cut(s) 625, 736
Bsc4I CCNNNNNNNGG 3 cut(s) 289, 739, 1486
Bse1I ACTGG 1 cut(s) 236
Bse21I CCTNAGG 1 cut(s) 1257
Bse8I GATNNNNATC 2 cut(s) 75, 1038
BseAI TCCGGA 1 cut(s) 625
BseCI ATCGAT 1 cut(s) 383
BseDI CCNNGG 2 cut(s) 859, 1467
BseGI GGATG 4 cut(s) 10, 163, 463, 1267
BseJI GATNNNNATC 2 cut(s) 75, 1038
BseLI CCNNNNNNNGG 3 cut(s) 289, 739, 1486
BseMII CTCAG 6 cut(s) 236, 470, 897, 1152, 1248, 1362
BseNI ACTGG 1 cut(s) 236
BseRI GAGGAG 2 cut(s) 737, 1121
BseX3I CGGCCG 1 cut(s) 393
BseXI GCAGC 2 cut(s) 190, 1209
Bsh1236I CGCG 1 cut(s) 959
Bsh1285I CGRYCG 1 cut(s) 396
BshFI GGCC 4 cut(s) 395, 462, 1433, 1479
BshVI ATCGAT 1 cut(s) 383
BsiEI CGRYCG 1 cut(s) 396
BsiHKAI GWGCWC 1 cut(s) 1293
BsiHKCI CYCGRG 1 cut(s) 1319
BsiSI CCGG 4 cut(s) 283, 626, 737, 1004
BsiWI CGTACG 1 cut(s) 585
BslFI GGGAC 1 cut(s) 471
BslI CCNNNNNNNGG 3 cut(s) 289, 739, 1486
BsmAI GTCTC 4 cut(s) 656, 743, 835, 1337
BsmBI CGTCTC 1 cut(s) 743
BsmFI GGGAC 1 cut(s) 471
BsnI GGCC 4 cut(s) 395, 462, 1433, 1479
Bso31I GGTCTC 2 cut(s) 656, 835
BsoBI CYCGRG 1 cut(s) 1319
Bsp119I TTCGAA 2 cut(s) 930, 1079
Bsp1286I GDGCHC 1 cut(s) 1293
Bsp13I TCCGGA 1 cut(s) 625
Bsp1720I GCTNAGC 1 cut(s) 883
BspACI CCGC 8 cut(s) 201, 351, 396, 402, 494, 506, 509, 1225
BspANI GGCC 4 cut(s) 395, 462, 1433, 1479
BspCNI CTCAG 6 cut(s) 235, 471, 896, 1153, 1249, 1363
BspDI ATCGAT 1 cut(s) 383
BspEI TCCGGA 1 cut(s) 625
BspFNI CGCG 1 cut(s) 959
BspLI GGNNCC 3 cut(s) 281, 463, 1002
BspMI ACCTGC 1 cut(s) 1207
BspPI GGATC 4 cut(s) 274, 287, 388, 1014
BspT104I TTCGAA 2 cut(s) 930, 1079
BspTI CTTAAG 1 cut(s) 1271
BspTNI GGTCTC 2 cut(s) 656, 835
BsrI ACTGG 1 cut(s) 236
BssECI CCNNGG 2 cut(s) 859, 1467
BssT1I CCWWGG 2 cut(s) 859, 1467
Bst4CI ACNGT 5 cut(s) 121, 734, 746, 754, 1152
Bst6I CTCTTC 2 cut(s) 84, 129
BstAFI CTTAAG 1 cut(s) 1271
BstAPI GCANNNNNTGC 1 cut(s) 1222
BstBI TTCGAA 2 cut(s) 930, 1079
BstC8I GCNNGC 3 cut(s) 208, 400, 788
BstDEI CTNAG 6 cut(s) 222, 479, 883, 1161, 1257, 1371
BstENI CCTNNNNNAGG 1 cut(s) 1484
BstF5I GGATG 4 cut(s) 10, 163, 463, 1267
BstFNI CGCG 1 cut(s) 959
BstHHI GCGC 2 cut(s) 799, 1340
BstMAI GTCTC 4 cut(s) 656, 743, 835, 1337
BstMCI CGRYCG 1 cut(s) 396
BstMWI GCNNNNNNNGC 5 cut(s) 212, 796, 965, 1120, 1222
BstNSI RCATGY 2 cut(s) 22, 1255
BstUI CGCG 1 cut(s) 959
BstV1I GCAGC 2 cut(s) 190, 1209
BstV2I GAAGAC 2 cut(s) 279, 1068
BstX2I RGATCY 1 cut(s) 279
BstYI RGATCY 1 cut(s) 279
BstZI CGGCCG 1 cut(s) 393
Bsu15I ATCGAT 1 cut(s) 383
Bsu36I CCTNAGG 1 cut(s) 1257
BsuRI GGCC 4 cut(s) 395, 462, 1433, 1479
BsuTUI ATCGAT 1 cut(s) 383
BtgZI GCGATG 1 cut(s) 1072
BtsCI GGATG 4 cut(s) 10, 163, 463, 1267
BtsI GCAGTG 1 cut(s) 806
BtsIMutI CAGTG 3 cut(s) 117, 165, 806
BveI ACCTGC 1 cut(s) 1207
Cac8I GCNNGC 3 cut(s) 208, 400, 788
CaiI CAGNNNCTG 2 cut(s) 1156, 1222
CfoI GCGC 2 cut(s) 799, 1340
Cfr13I GGNCC 3 cut(s) 461, 515, 1431
ClaI ATCGAT 1 cut(s) 383
CseI GACGC 1 cut(s) 1256
Csp6I GTAC 8 cut(s) 22, 182, 553, 562, 586, 637, 717, 1312
CviAII CATG 5 cut(s) 19, 1191, 1252, 1304, 1397
CviQI GTAC 8 cut(s) 22, 182, 553, 562, 586, 637, 717, 1312
DdeI CTNAG 6 cut(s) 222, 479, 883, 1161, 1257, 1371
EaeI YGGCCR 1 cut(s) 393
EagI CGGCCG 1 cut(s) 393
Eam1104I CTCTTC 2 cut(s) 84, 129
EarI CTCTTC 2 cut(s) 84, 129
EclXI CGGCCG 1 cut(s) 393
Eco130I CCWWGG 2 cut(s) 859, 1467
Eco31I GGTCTC 2 cut(s) 656, 835
Eco47I GGWCC 1 cut(s) 515
Eco52I CGGCCG 1 cut(s) 393
Eco57I CTGAAG 3 cut(s) 189, 1004, 1040
Eco81I CCTNAGG 1 cut(s) 1257
Eco88I CYCGRG 1 cut(s) 1319
EcoNI CCTNNNNNAGG 1 cut(s) 1484
EcoT14I CCWWGG 2 cut(s) 859, 1467
EcoT22I ATGCAT 2 cut(s) 1192, 1402
ErhI CCWWGG 2 cut(s) 859, 1467
Esp3I CGTCTC 1 cut(s) 743
FaeI CATG 5 cut(s) 22, 1194, 1255, 1307, 1400
FalI AAGNNNNNCTT 2 cut(s) 1376, 1408
FaqI GGGAC 1 cut(s) 471
FatI CATG 5 cut(s) 18, 1190, 1251, 1303, 1396
FauNDI CATATG 1 cut(s) 873
FbaI TGATCA 2 cut(s) 76, 240
Fnu4HI GCNGC 4 cut(s) 204, 396, 507, 1223
FokI GGATG 4 cut(s) 17, 170, 470, 1274
Fsp4HI GCNGC 4 cut(s) 204, 396, 507, 1223
FspBI CTAG 1 cut(s) 945
FspI TGCGCA 1 cut(s) 798
GlaI GCGC 2 cut(s) 798, 1339
GluI GCNGC 4 cut(s) 204, 396, 507, 1223
GsuI CTGGAG 1 cut(s) 448
HaeIII GGCC 4 cut(s) 395, 462, 1433, 1479
HapII CCGG 4 cut(s) 283, 626, 737, 1004
HgaI GACGC 1 cut(s) 1256
HhaI GCGC 2 cut(s) 799, 1340
Hin1II CATG 5 cut(s) 22, 1194, 1255, 1307, 1400
Hin6I GCGC 2 cut(s) 797, 1338
HinP1I GCGC 2 cut(s) 797, 1338
HincII GTYRAC 1 cut(s) 814
HindII GTYRAC 1 cut(s) 814
HindIII AAGCTT 1 cut(s) 146
HinfI GANTC 4 cut(s) 95, 727, 847, 1097
HpaI GTTAAC 1 cut(s) 814
HpaII CCGG 4 cut(s) 283, 626, 737, 1004
HphI GGTGA 4 cut(s) 256, 722, 766, 860
Hpy166II GTNNAC 4 cut(s) 264, 742, 814, 1492
Hpy188I TCNGA 7 cut(s) 126, 330, 852, 1011, 1162, 1372, 1486
Hpy188III TCNNGA 6 cut(s) 152, 488, 518, 626, 1321, 1450
Hpy8I GTNNAC 4 cut(s) 264, 742, 814, 1492
Hpy99I CGWCG 6 cut(s) 41, 44, 47, 68, 257, 593
HpyAV CCTTC 3 cut(s) 979, 1015, 1075
HpyCH4III ACNGT 5 cut(s) 121, 734, 746, 754, 1152
HpyCH4IV ACGT 2 cut(s) 237, 588
HpyCH4V TGCA 6 cut(s) 837, 1190, 1297, 1307, 1354, 1400
HpyF10VI GCNNNNNNNGC 5 cut(s) 212, 796, 965, 1120, 1222
HpyF3I CTNAG 6 cut(s) 222, 479, 883, 1161, 1257, 1371
HpySE526I ACGT 2 cut(s) 237, 588
Hsp92II CATG 5 cut(s) 22, 1194, 1255, 1307, 1400
HspAI GCGC 2 cut(s) 797, 1338
Kpn2I TCCGGA 1 cut(s) 625
Ksp22I TGATCA 2 cut(s) 76, 240
KspAI GTTAAC 1 cut(s) 814
LmnI GCTCC 5 cut(s) 628, 892, 1000, 1132, 1230
Lsp1109I GCAGC 2 cut(s) 190, 1209
LweI GCATC 3 cut(s) 148, 465, 1177
MaeI CTAG 1 cut(s) 945
MaeII ACGT 2 cut(s) 237, 588
MaeIII GTNAC 6 cut(s) 59, 434, 728, 866, 953, 1459
MflI RGATCY 1 cut(s) 279
MhlI GDGCHC 1 cut(s) 1293
MluCI AATT 7 cut(s) 320, 712, 926, 1174, 1203, 1349, 1379
MluI ACGCGT 1 cut(s) 957
MlyI GAGTC 2 cut(s) 736, 1106
MmeI TCCRAC 2 cut(s) 282, 972
Mph1103I ATGCAT 2 cut(s) 1192, 1402
MroI TCCGGA 1 cut(s) 625
MseI TTAA 4 cut(s) 813, 1272, 1382, 1409
MslI CAYNNNNRTG 2 cut(s) 170, 1050
MspA1I CMGCKG 1 cut(s) 203
MspCI CTTAAG 1 cut(s) 1271
MspI CCGG 4 cut(s) 283, 626, 737, 1004
MvnI CGCG 1 cut(s) 959
MwoI GCNNNNNNNGC 5 cut(s) 212, 796, 965, 1120, 1222
NdeI CATATG 1 cut(s) 873
NlaIII CATG 5 cut(s) 22, 1194, 1255, 1307, 1400
NlaIV GGNNCC 3 cut(s) 281, 463, 1002
NmeAIII GCCGAG 1 cut(s) 1366
NmuCI GTSAC 3 cut(s) 59, 728, 866
NsbI TGCGCA 1 cut(s) 798
NsiI ATGCAT 2 cut(s) 1192, 1402
NspI RCATGY 2 cut(s) 22, 1255
NspV TTCGAA 2 cut(s) 930, 1079
OliI CACNNNNGTG 1 cut(s) 170
PaeR7I CTCGAG 1 cut(s) 1319
PaqCI CACCTGC 1 cut(s) 1207
PciI ACATGT 1 cut(s) 18
PcsI WCGNNNNNNNCGW 1 cut(s) 51
PfeI GAWTC 2 cut(s) 95, 847
Pfl23II CGTACG 1 cut(s) 585
PkrI GCNGC 4 cut(s) 205, 397, 508, 1224
PleI GAGTC 2 cut(s) 735, 1105
PpsI GAGTC 2 cut(s) 735, 1105
PscI ACATGT 1 cut(s) 18
PspLI CGTACG 1 cut(s) 585
PspN4I GGNNCC 3 cut(s) 281, 463, 1002
PspPI GGNCC 3 cut(s) 461, 515, 1431
PstNI CAGNNNCTG 2 cut(s) 1156, 1222
PsuI RGATCY 1 cut(s) 279
RsaI GTAC 8 cut(s) 23, 183, 554, 563, 587, 638, 718, 1313
RsaNI GTAC 8 cut(s) 22, 182, 553, 562, 586, 637, 717, 1312
RseI CAYNNNNRTG 2 cut(s) 170, 1050
SaqAI TTAA 4 cut(s) 813, 1272, 1382, 1409
SatI GCNGC 4 cut(s) 204, 396, 507, 1223
Sau96I GGNCC 3 cut(s) 461, 515, 1431
SchI GAGTC 2 cut(s) 736, 1106
SduI GDGCHC 1 cut(s) 1293
SfaNI GCATC 3 cut(s) 148, 465, 1177
Sfr274I CTCGAG 1 cut(s) 1319
SfuI TTCGAA 2 cut(s) 930, 1079
SinI GGWCC 1 cut(s) 515
SlaI CTCGAG 1 cut(s) 1319
SmiMI CAYNNNNRTG 2 cut(s) 170, 1050
SmlI CTYRAG 2 cut(s) 1271, 1319
SmoI CTYRAG 2 cut(s) 1271, 1319
Sse9I AATT 7 cut(s) 320, 712, 926, 1174, 1203, 1349, 1379
SsiI CCGC 8 cut(s) 201, 351, 396, 402, 494, 506, 509, 1225
SspMI CTAG 1 cut(s) 945
StyI CCWWGG 2 cut(s) 859, 1467
TaaI ACNGT 5 cut(s) 121, 734, 746, 754, 1152
TaiI ACGT 2 cut(s) 240, 591
TaqI TCGA 9 cut(s) 83, 383, 542, 591, 930, 1079, 1095, 1320, 1416
TaqII GACCGA 2 cut(s) 520, 859
TasI AATT 7 cut(s) 320, 712, 926, 1174, 1203, 1349, 1379
TatI WGTACW 2 cut(s) 181, 1311
TauI GCSGC 2 cut(s) 398, 509
TfiI GAWTC 2 cut(s) 95, 847
Tru1I TTAA 4 cut(s) 813, 1272, 1382, 1409
Tru9I TTAA 4 cut(s) 813, 1272, 1382, 1409
TscAI CASTG 3 cut(s) 124, 172, 806
TseFI GTSAC 3 cut(s) 59, 728, 866
TseI GCWGC 2 cut(s) 203, 1222
Tsp45I GTSAC 3 cut(s) 59, 728, 866
TspDTI ATGAA 4 cut(s) 285, 785, 1034, 1168
TspGWI ACGGA 1 cut(s) 734
TspRI CASTG 3 cut(s) 124, 172, 806
Vha464I CTTAAG 1 cut(s) 1271
VpaK11BI GGWCC 1 cut(s) 515
XagI CCTNNNNNAGG 1 cut(s) 1484
XapI RAATTY 1 cut(s) 926
XceI RCATGY 2 cut(s) 22, 1255
XcmI CCANNNNNNNNNTGG 1 cut(s) 1126
XhoI CTCGAG 1 cut(s) 1319
XspI CTAG 1 cut(s) 945
Zsp2I ATGCAT 2 cut(s) 1192, 1402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.