pycom08g03020

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0000864
Physical Location & Seq
Forward (+)
2374981 .. 2376600
1620 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g03020.1

Sequence Viewer

Length: 1620 bp
ATGGACAAGAAACGCAACCGGAACGGTGACAACTACATCGTCATAAACAGCGATGAAGAATCAGATCAATCAGAACGTTTTGAAGAAGAAGAAGAAGACGACGCAGTCGATGATGAATTTGCTTTTGTCCAAGAAGACAACATCGACAGCACCGATCACTACGAGTTCAGCGAAAGTACTGATTCGTCTCATAATCCATATCAAAATTACGCCATATTGAAAGAGGAAGATATTCGCCAACTGCAGGAGGAAGACATCACAACAGTTTCTACAATCCTTTCCATACCGAAATCCGCTGCATGCTTCCTCCTCTCACACTTCAACTGGAGCGTCGGCAAAGTCAACGAGGAGTGGTTCGCCGACGAAGATAGAGTCAGAGAAAAGGTAGGGTTGTTGAGGAAGCCCGTGGTTAGTTTCCATCCCACAGATTTGATTACTTGCGGAATATGTTTTGATGATCATCAGTATGGATCAAAGGATGTTTTTTTCGCTGTTTGCGGTCATCCCTTTTGTCGGGATTGCTGGACAGGTTATATTAAATCAGCGATAGATGAGGGTCCGGCGTGTTTGATGGTGCTGAGATGTCCCGAACCGTCTTGCAAGGCAGCGGTTGGACCGGATTTAATCGAGGGTTTGACGACAAGAGAAGATCATGAGAGGTATAAGTACTTCCTTTTGAGATCTTACGTTGAAGGGCGCAGGGAGATCAAGTGGTGCCCTGCACCGGATTGCAAGTACGCTGTGAAATTCAATGCAGGTGGAACCAAAAGCTATGACGTTTCGTGCCTTTGCTTGTACGGGTTTTGCTGGAATTGCAATGAGGAAATTCACCGTCCTGTGAACTGCAAGACTGTGGAAAATTGGATTTCGAAGAACAAGGACGACTCGATGAATGCGCAGTGGATACTTGGGAATACCAAGCCTTGCCCCAAGTGCAAGAAACCAATCGAGAAGAACAACGGGTGTAATCATATGACTTGCGCAAACCCTTGCAGGTATGAATTTTGCTGGTATTGCCTCGAGAATTATACTGGACATACATGCTGCAATGGATATAGCAGGGCAACTGATGAATATACGGGAACTGACCCGGACAAGGTTGAAGCTGGGAGGGATAAAAAGAGGGCAAAGAGATACGTGGAGAAATACCTACATTATTATGAACGATGGGCAAACAATCTGTCTTCGAAGAAAAAAGCAATAGAGGATTTACATAATGTGCAGAGCAAGGACATTACGAAGCTTGCCGCCATACAATGTCAATCGGAGCTTCAGCTCAAGTTTATCGTAGAGGCGTGGCGGCAGATCATTGAATGTAGGCAGGTATTGCGATGGACGTATGCATACGGGTACTACATTCCCGAGAATGAAGAACTGAAGATTCGGCTATTCGAGTACTTGCAAGGCCACGCAGAGTTCAGCCTGGAGAGGCTTCACAAATGTGCTGAGAGTGAGATGAAAGAGTTTCTCTTTGCTAAAGGTCCGTTGGAAAGTTTCCGGGACTTTCGTATAAAGCTCGTAAGACTGACAAAGGTGACTGCAAGTTATTTTGAGAAGCTGGTTAGAGCGTTGGAGAATGATCTTTCTGAATTGGACATACAGGGGATTCACAAGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IBR zf-RanBP

Protein Analysis

540

Amino Acids

62.83

Weight (kDa)

5.39

Isoelectric Point (pI)

48.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UBA_ARI1 PF21235 80 - 119 3.2e-10 E3 ubiquitin-protein ligase ARIH1, UBA-like domain
IBR PF01485 219 - 282 1.7e-16 IBR domain, a half RING-finger domain
IBR_1 PF22191 305 - 346 2.3e-11 IBR domain
IBR_2 PF22605 305 - 344 2.4e-09 IBR domain
Ariadne PF19422 382 - 530 4.6e-06 Ariadne domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 746
AasI GACNNNNNNGTC 1 cut(s) 104
Acc16I TGCGCA 2 cut(s) 897, 982
Acc36I ACCTGC 3 cut(s) 746, 984, 1312
AccB1I GGYRCC 1 cut(s) 714
AciI CCGC 6 cut(s) 294, 441, 498, 608, 1248, 1300
AclI AACGTT 1 cut(s) 76
AclWI GGATC 1 cut(s) 478
AcsI RAATTY 4 cut(s) 116, 746, 825, 1001
AcuI CTGAAG 2 cut(s) 1256, 1397
AfaI GTAC 6 cut(s) 178, 668, 737, 797, 1352, 1397
AfiI CCNNNNNNNGG 4 cut(s) 244, 513, 724, 1096
AgsI TTSAA 7 cut(s) 83, 220, 322, 692, 751, 1103, 1313
AjnI CCWGG 1 cut(s) 1422
AleI CACNNNNGTG 1 cut(s) 1440
AluBI AGCT 7 cut(s) 771, 1106, 1243, 1270, 1276, 1516, 1558
AluI AGCT 7 cut(s) 771, 1106, 1243, 1270, 1276, 1516, 1558
Alw26I GTCTC 1 cut(s) 192
AlwI GGATC 1 cut(s) 478
Ama87I CYCGRG 2 cut(s) 1019, 1361
AoxI GGCC 1 cut(s) 1405
ApeKI GCWGC 3 cut(s) 296, 605, 1044
ApoI RAATTY 4 cut(s) 116, 746, 825, 1001
ArsI GACNNNNNNTTYG 4 cut(s) 357, 389, 1166, 1198
Asp700I GAANNNNTTC 1 cut(s) 231
AspLEI GCGC 3 cut(s) 699, 898, 983
AspS9I GGNCC 3 cut(s) 557, 614, 1481
AsuC2I CCSGG 2 cut(s) 1091, 1499
AsuHPI GGTGA 3 cut(s) 38, 821, 1546
AsuII TTCGAA 2 cut(s) 869, 1187
AvaI CYCGRG 2 cut(s) 1019, 1361
AvaII GGWCC 3 cut(s) 557, 614, 1481
BaeGI GKGCMC 1 cut(s) 719
BanI GGYRCC 1 cut(s) 714
BarI GAAGNNNNNNTAC 2 cut(s) 653, 685
BbsI GAAGAC 4 cut(s) 102, 141, 258, 1176
BbvI GCAGC 3 cut(s) 283, 617, 1031
BccI CCATC 4 cut(s) 426, 565, 1161, 1326
BciT130I CCWGG 1 cut(s) 1424
BciVI GTATCC 1 cut(s) 897
BclI TGATCA 1 cut(s) 457
BcnI CCSGG 2 cut(s) 1091, 1499
BcoDI GTCTC 1 cut(s) 192
BfmI CTRYAG 1 cut(s) 242
BfuAI ACCTGC 3 cut(s) 746, 984, 1312
BfuI GTATCC 1 cut(s) 897
BglII AGATCT 1 cut(s) 680
BisI GCNGC 5 cut(s) 297, 606, 1045, 1248, 1301
BlsI GCNGC 5 cut(s) 298, 607, 1046, 1249, 1302
BmcAI AGTACT 3 cut(s) 178, 668, 1397
Bme1390I CCNGG 3 cut(s) 1091, 1424, 1499
Bme18I GGWCC 3 cut(s) 557, 614, 1481
BmeT110I CYCGRG 2 cut(s) 1019, 1361
BmgT120I GGNCC 3 cut(s) 557, 614, 1481
BmiI GGNNCC 3 cut(s) 558, 716, 763
BmrFI CCNGG 3 cut(s) 1091, 1424, 1499
BpiI GAAGAC 4 cut(s) 102, 141, 258, 1176
BpmI CTGGAG 2 cut(s) 346, 1445
Bpu14I TTCGAA 2 cut(s) 869, 1187
BpuEI CTTGAG 1 cut(s) 1262
BpuMI CCSGG 2 cut(s) 1091, 1499
BsaAI YACGTR 1 cut(s) 1138
BsaBI GATNNNNATC 1 cut(s) 459
BsaJI CCNNGG 1 cut(s) 405
BsaWI WCCGGW 3 cut(s) 18, 616, 724
BsaXI ACNNNNNCTCC 2 cut(s) 695, 725
Bsc4I CCNNNNNNNGG 4 cut(s) 244, 513, 724, 1096
Bse1I ACTGG 2 cut(s) 329, 1036
Bse3DI GCAATG 2 cut(s) 823, 1054
Bse8I GATNNNNATC 1 cut(s) 459
BseBI CCWGG 1 cut(s) 1424
BseDI CCNNGG 1 cut(s) 405
BseGI GGATG 3 cut(s) 418, 484, 502
BseJI GATNNNNATC 1 cut(s) 459
BseLI CCNNNNNNNGG 4 cut(s) 244, 513, 724, 1096
BseMI GCAATG 2 cut(s) 823, 1054
BseMII CTCAG 2 cut(s) 569, 1437
BseNI ACTGG 2 cut(s) 329, 1036
BseRI GAGGAG 2 cut(s) 299, 362
BseSI GKGCMC 1 cut(s) 719
BseXI GCAGC 3 cut(s) 283, 617, 1031
BseYI CCCAGC 1 cut(s) 1106
BsgI GTGCAG 2 cut(s) 705, 1241
BshFI GGCC 1 cut(s) 1407
BshNI GGYRCC 1 cut(s) 714
BsiHKCI CYCGRG 2 cut(s) 1019, 1361
BsiSI CCGG 6 cut(s) 19, 560, 617, 725, 1091, 1498
BslFI GGGAC 2 cut(s) 570, 1514
BslI CCNNNNNNNGG 4 cut(s) 244, 513, 724, 1096
BsmAI GTCTC 1 cut(s) 192
BsmBI CGTCTC 1 cut(s) 192
BsmFI GGGAC 2 cut(s) 570, 1514
BsmI GAATGC 1 cut(s) 898
BsnI GGCC 1 cut(s) 1407
BsoBI CYCGRG 2 cut(s) 1019, 1361
Bsp119I TTCGAA 2 cut(s) 869, 1187
Bsp1286I GDGCHC 1 cut(s) 719
Bsp143I GATC 9 cut(s) 64, 154, 457, 470, 649, 680, 705, 1305, 1579
BspACI CCGC 6 cut(s) 294, 441, 498, 608, 1248, 1300
BspANI GGCC 1 cut(s) 1407
BspCNI CTCAG 2 cut(s) 570, 1438
BspHI TCATGA 1 cut(s) 652
BspLI GGNNCC 3 cut(s) 558, 716, 763
BspMAI CTGCAG 1 cut(s) 246
BspMI ACCTGC 3 cut(s) 746, 984, 1312
BspPI GGATC 1 cut(s) 478
BspT104I TTCGAA 2 cut(s) 869, 1187
BspT107I GGYRCC 1 cut(s) 714
BsrDI GCAATG 2 cut(s) 823, 1054
BsrI ACTGG 2 cut(s) 329, 1036
BssECI CCNNGG 1 cut(s) 405
BssMI GATC 9 cut(s) 64, 154, 457, 470, 649, 680, 705, 1305, 1579
Bst2UI CCWGG 1 cut(s) 1424
Bst4CI ACNGT 5 cut(s) 26, 265, 594, 833, 853
BstAPI GCANNNNNTGC 1 cut(s) 1327
BstBAI YACGTR 1 cut(s) 1138
BstBI TTCGAA 2 cut(s) 869, 1187
BstC8I GCNNGC 2 cut(s) 301, 1245
BstDEI CTNAG 2 cut(s) 578, 1446
BstDSI CCRYGG 1 cut(s) 405
BstF5I GGATG 3 cut(s) 418, 484, 502
BstHHI GCGC 3 cut(s) 699, 898, 983
BstKTI GATC 9 cut(s) 67, 157, 460, 473, 652, 683, 708, 1308, 1582
BstMAI GTCTC 1 cut(s) 192
BstMBI GATC 9 cut(s) 64, 154, 457, 470, 649, 680, 705, 1305, 1579
BstMWI GCNNNNNNNGC 4 cut(s) 813, 933, 1014, 1327
BstNI CCWGG 1 cut(s) 1424
BstNSI RCATGY 2 cut(s) 303, 1044
BstSCI CCNGG 3 cut(s) 1089, 1422, 1497
BstSFI CTRYAG 1 cut(s) 242
BstSLI GKGCMC 1 cut(s) 719
BstV1I GCAGC 3 cut(s) 283, 617, 1031
BstV2I GAAGAC 4 cut(s) 102, 141, 258, 1176
BstX2I RGATCY 1 cut(s) 680
BstYI RGATCY 1 cut(s) 680
BsuI GTATCC 1 cut(s) 897
BsuRI GGCC 1 cut(s) 1407
BtgI CCRYGG 1 cut(s) 405
BtgZI GCGATG 2 cut(s) 66, 1345
BtsCI GGATG 3 cut(s) 418, 484, 502
BtsI GCAGTG 1 cut(s) 905
BtsIMutI CAGTG 1 cut(s) 905
BveI ACCTGC 3 cut(s) 746, 984, 1312
Cac8I GCNNGC 2 cut(s) 301, 1245
CciI TCATGA 1 cut(s) 652
CfoI GCGC 3 cut(s) 699, 898, 983
Cfr13I GGNCC 3 cut(s) 557, 614, 1481
CseI GACGC 2 cut(s) 110, 319
Csp6I GTAC 6 cut(s) 177, 667, 736, 796, 1351, 1396
CspCI CAANNNNNGTGG 2 cut(s) 739, 774
CviAII CATG 3 cut(s) 300, 653, 1041
CviQI GTAC 6 cut(s) 177, 667, 736, 796, 1351, 1396
DdeI CTNAG 2 cut(s) 578, 1446
DpnI GATC 9 cut(s) 66, 156, 459, 472, 651, 682, 707, 1307, 1581
DpnII GATC 9 cut(s) 64, 154, 457, 470, 649, 680, 705, 1305, 1579
DrdI GACNNNNNNGTC 1 cut(s) 104
DseDI GACNNNNNNGTC 1 cut(s) 104
Eco47I GGWCC 3 cut(s) 557, 614, 1481
Eco57I CTGAAG 2 cut(s) 1256, 1397
Eco88I CYCGRG 2 cut(s) 1019, 1361
EcoRII CCWGG 1 cut(s) 1422
EcoT22I ATGCAT 1 cut(s) 1345
Esp3I CGTCTC 1 cut(s) 192
FaeI CATG 3 cut(s) 303, 656, 1044
FaqI GGGAC 2 cut(s) 570, 1514
FatI CATG 3 cut(s) 299, 652, 1040
FauNDI CATATG 1 cut(s) 972
FbaI TGATCA 1 cut(s) 457
Fnu4HI GCNGC 5 cut(s) 297, 606, 1045, 1248, 1301
FokI GGATG 3 cut(s) 405, 489, 491
Fsp4HI GCNGC 5 cut(s) 297, 606, 1045, 1248, 1301
FspI TGCGCA 2 cut(s) 897, 982
GlaI GCGC 3 cut(s) 698, 897, 982
GluI GCNGC 5 cut(s) 297, 606, 1045, 1248, 1301
GsaI CCCAGC 1 cut(s) 1110
GsuI CTGGAG 2 cut(s) 346, 1445
HaeIII GGCC 1 cut(s) 1407
HapII CCGG 6 cut(s) 19, 560, 617, 725, 1091, 1498
HgaI GACGC 2 cut(s) 110, 319
HhaI GCGC 3 cut(s) 699, 898, 983
Hin1II CATG 3 cut(s) 303, 656, 1044
Hin6I GCGC 3 cut(s) 697, 896, 981
HinP1I GCGC 3 cut(s) 697, 896, 981
HincII GTYRAC 1 cut(s) 343
HindII GTYRAC 1 cut(s) 343
HindIII AAGCTT 1 cut(s) 1241
HinfI GANTC 6 cut(s) 59, 182, 372, 884, 1381, 1606
HpaII CCGG 6 cut(s) 19, 560, 617, 725, 1091, 1498
HphI GGTGA 3 cut(s) 38, 821, 1546
Hpy166II GTNNAC 2 cut(s) 343, 841
Hpy188I TCNGA 5 cut(s) 64, 73, 377, 1267, 1588
Hpy188III TCNNGA 7 cut(s) 515, 587, 653, 949, 1021, 1361, 1617
Hpy8I GTNNAC 2 cut(s) 343, 841
Hpy99I CGWCG 3 cut(s) 104, 335, 365
HpyAV CCTTC 1 cut(s) 686
HpyCH4III ACNGT 5 cut(s) 26, 265, 594, 833, 853
HpyCH4IV ACGT 5 cut(s) 76, 687, 777, 1137, 1337
HpyF10VI GCNNNNNNNGC 4 cut(s) 813, 933, 1014, 1327
HpyF3I CTNAG 2 cut(s) 578, 1446
HpySE526I ACGT 5 cut(s) 76, 687, 777, 1137, 1337
Hsp92II CATG 3 cut(s) 303, 656, 1044
HspAI GCGC 3 cut(s) 697, 896, 981
Ksp22I TGATCA 1 cut(s) 457
Kzo9I GATC 9 cut(s) 64, 154, 457, 470, 649, 680, 705, 1305, 1579
LmnI GCTCC 2 cut(s) 327, 1267
Lsp1109I GCAGC 3 cut(s) 283, 617, 1031
MaeII ACGT 5 cut(s) 76, 687, 777, 1137, 1337
MaeIII GTNAC 2 cut(s) 26, 1534
MalI GATC 9 cut(s) 66, 156, 459, 472, 651, 682, 707, 1307, 1581
MboI GATC 9 cut(s) 64, 154, 457, 470, 649, 680, 705, 1305, 1579
MflI RGATCY 1 cut(s) 680
MhlI GDGCHC 1 cut(s) 719
MluCI AATT 9 cut(s) 116, 205, 746, 811, 825, 859, 1001, 1024, 1589
MlyI GAGTC 2 cut(s) 381, 878
MmeI TCCRAC 3 cut(s) 592, 1467, 1551
Mph1103I ATGCAT 1 cut(s) 1345
MroXI GAANNNNTTC 1 cut(s) 231
MseI TTAA 2 cut(s) 537, 623
MslI CAYNNNNRTG 3 cut(s) 465, 1158, 1440
MspA1I CMGCKG 2 cut(s) 296, 608
MspI CCGG 6 cut(s) 19, 560, 617, 725, 1091, 1498
MspR9I CCNGG 3 cut(s) 1091, 1424, 1499
Mva1269I GAATGC 1 cut(s) 898
MvaI CCWGG 1 cut(s) 1424
MwoI GCNNNNNNNGC 4 cut(s) 813, 933, 1014, 1327
NciI CCSGG 2 cut(s) 1091, 1499
NdeI CATATG 1 cut(s) 972
NdeII GATC 9 cut(s) 64, 154, 457, 470, 649, 680, 705, 1305, 1579
NlaIII CATG 3 cut(s) 303, 656, 1044
NlaIV GGNNCC 3 cut(s) 558, 716, 763
NmuCI GTSAC 2 cut(s) 26, 1534
NsbI TGCGCA 2 cut(s) 897, 982
NsiI ATGCAT 1 cut(s) 1345
NspI RCATGY 2 cut(s) 303, 1044
NspV TTCGAA 2 cut(s) 869, 1187
OliI CACNNNNGTG 1 cut(s) 1440
PaeI GCATGC 1 cut(s) 303
PaeR7I CTCGAG 1 cut(s) 1019
PagI TCATGA 1 cut(s) 652
PaqCI CACCTGC 1 cut(s) 746
PcsI WCGNNNNNNNCGW 3 cut(s) 105, 150, 168
PctI GAATGC 1 cut(s) 898
PdmI GAANNNNTTC 1 cut(s) 231
PfeI GAWTC 4 cut(s) 59, 182, 1381, 1606
PflFI GACNNNGTC 1 cut(s) 104
PfoI TCCNGGA 1 cut(s) 1497
PkrI GCNGC 5 cut(s) 298, 607, 1046, 1249, 1302
PleI GAGTC 2 cut(s) 380, 878
PpsI GAGTC 2 cut(s) 380, 878
Ppu21I YACGTR 1 cut(s) 1138
Psp1406I AACGTT 1 cut(s) 76
Psp6I CCWGG 1 cut(s) 1422
PspFI CCCAGC 1 cut(s) 1106
PspGI CCWGG 1 cut(s) 1422
PspN4I GGNNCC 3 cut(s) 558, 716, 763
PspPI GGNCC 3 cut(s) 557, 614, 1481
PstI CTGCAG 1 cut(s) 246
PsuI RGATCY 1 cut(s) 680
PsyI GACNNNGTC 1 cut(s) 104
RsaI GTAC 6 cut(s) 178, 668, 737, 797, 1352, 1397
RsaNI GTAC 6 cut(s) 177, 667, 736, 796, 1351, 1396
RseI CAYNNNNRTG 3 cut(s) 465, 1158, 1440
SaqAI TTAA 2 cut(s) 537, 623
SatI GCNGC 5 cut(s) 297, 606, 1045, 1248, 1301
Sau3AI GATC 9 cut(s) 64, 154, 457, 470, 649, 680, 705, 1305, 1579
Sau96I GGNCC 3 cut(s) 557, 614, 1481
ScaI AGTACT 3 cut(s) 178, 668, 1397
SchI GAGTC 2 cut(s) 381, 878
ScrFI CCNGG 3 cut(s) 1091, 1424, 1499
SduI GDGCHC 1 cut(s) 719
SfcI CTRYAG 1 cut(s) 242
Sfr274I CTCGAG 1 cut(s) 1019
SfuI TTCGAA 2 cut(s) 869, 1187
SinI GGWCC 3 cut(s) 557, 614, 1481
SlaI CTCGAG 1 cut(s) 1019
SmiMI CAYNNNNRTG 3 cut(s) 465, 1158, 1440
SmlI CTYRAG 2 cut(s) 1019, 1277
SmoI CTYRAG 2 cut(s) 1019, 1277
SphI GCATGC 1 cut(s) 303
Sse9I AATT 9 cut(s) 116, 205, 746, 811, 825, 859, 1001, 1024, 1589
SsiI CCGC 6 cut(s) 294, 441, 498, 608, 1248, 1300
StyD4I CCNGG 3 cut(s) 1089, 1422, 1497
TaaI ACNGT 5 cut(s) 26, 265, 594, 833, 853
TaiI ACGT 5 cut(s) 79, 690, 780, 1140, 1340
TaqI TCGA 9 cut(s) 108, 144, 627, 869, 887, 948, 1020, 1187, 1392
TasI AATT 9 cut(s) 116, 205, 746, 811, 825, 859, 1001, 1024, 1589
TatI WGTACW 3 cut(s) 176, 666, 1395
TauI GCSGC 2 cut(s) 1250, 1303
TfiI GAWTC 4 cut(s) 59, 182, 1381, 1606
Tru1I TTAA 2 cut(s) 537, 623
Tru9I TTAA 2 cut(s) 537, 623
TscAI CASTG 1 cut(s) 905
TseFI GTSAC 2 cut(s) 26, 1534
TseI GCWGC 3 cut(s) 296, 605, 1044
Tsp45I GTSAC 2 cut(s) 26, 1534
TspDTI ATGAA 8 cut(s) 69, 129, 905, 1014, 1086, 1176, 1383, 1472
TspGWI ACGGA 1 cut(s) 1473
TspRI CASTG 1 cut(s) 905
Tth111I GACNNNGTC 1 cut(s) 104
VpaK11BI GGWCC 3 cut(s) 557, 614, 1481
XapI RAATTY 4 cut(s) 116, 746, 825, 1001
XceI RCATGY 2 cut(s) 303, 1044
XhoI CTCGAG 1 cut(s) 1019
XmnI GAANNNNTTC 1 cut(s) 231
ZrmI AGTACT 3 cut(s) 178, 668, 1397
Zsp2I ATGCAT 1 cut(s) 1345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.