Rmu_sc0000010.1_g000012

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000010.1
OG0000864
Physical Location & Seq
Forward (+)
51060 .. 52683
1624 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000010.1_g000012.1.cds

Sequence Viewer

Length: 1392 bp
atgaacgatatgcaccatgagagtgatgacggttatgaatccgcagagagaggcgatgaagaagcaatgaattacgatgacgatcttctagtagatgataaagtcgatgactttggtcagagtaggtctccacaggaaaattatattgttttggaagaagaagaaattgcaaaacttcaggataattatattgcagaagtctcaaccgttctctccatatccaaatccgatgcatgtttactgctccctcacttccattggaggaggaagatcaagtggtgtcctgccccggattgtaactgcgcaattcagtttgatactttgggtggaatggtgtcatatgatgtttcatgtctttgctcgcatagctattgctggaattgtactgaggagaatcatcgtcctgtggattgcgaaactgtggggaagtggattttgaagaataaggatgattctcaaaatgcgcagtggatactggttaattccaagccttgcccccagtgcaaaagaccaattgagaagaaccaaggctgtaatcacttgacgtgcagtgctccctgtgggtaccagttttgctggttatgcctcgggccacataaaaattacagaggttgctattgcaatagttataggccggggtcagttgataataatcccgggacatctaggtatatgaatgagacggcaaggaagagggcaaagaagtatttagagagatatattcattactatgaacgctgggaaaacaatggaagatcaaagggaaaagctattgaggattttgataaggtgaagaccgagcacattaagcaacttggagagatacatgtaaacacaaaagagcatgattacgattttatcatagaggcctggcaacagattattgagtgcaggcaagtattgcgatggacttatgcatatggctactacatgcctcaggatgcttatgctaagaagcacctattcgagtgcttgcaaggccatgctgagttcagcctggaaaggcttcacaattgcgctgagagcgaactgcaacagtttctggtcttatcaccatcagcagcttctgaaactttcaaagagtttaaaacaaaactagtgggcctgacaaaggtgaccggaacttatttcaaaaggttggttactgcattggaggaggggcttccggagacgagccgatatggagctggggctgcttatgaggagactgattggacatgtgaccaatgtacctatatcaatgtagggtctgccgttacctgtgcagcatgttctgagcaaaatccacaaggttactggtcttgtgaccgctgtacgattttcaatccggtgacgacaaccaggtgttggatgtgtgatgatgccgatgctgatcagggtggggaagtttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IBR zf-RanBP

Protein Analysis

463

Amino Acids

53.28

Weight (kDa)

5.15

Isoelectric Point (pI)

59.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 304, 465
Acc65I GGTACC 1 cut(s) 564
AccB1I GGYRCC 1 cut(s) 564
AccB7I CCANNNNNTGG 1 cut(s) 1347
AccIII TCCGGA 1 cut(s) 1165
AciI CCGC 2 cut(s) 42, 1309
AcuI CTGAAG 1 cut(s) 161
AfaI GTAC 4 cut(s) 385, 566, 1231, 1315
AfiI CCNNNNNNNGG 2 cut(s) 1111, 1347
AflIII ACRYGT 2 cut(s) 826, 1217
AgsI TTSAA 4 cut(s) 439, 1078, 1132, 1324
AhlI ACTAGT 1 cut(s) 1096
AjiI CACGTC 1 cut(s) 546
AjnI CCWGG 3 cut(s) 869, 996, 1340
AluBI AGCT 4 cut(s) 369, 770, 1064, 1187
AluI AGCT 4 cut(s) 369, 770, 1064, 1187
Alw21I GWGCWC 2 cut(s) 556, 804
Alw26I GTCTC 5 cut(s) 132, 205, 674, 1163, 1199
AlwNI CAGNNNCTG 2 cut(s) 1042, 1067
Ama87I CYCGRG 2 cut(s) 587, 656
Aor13HI TCCGGA 1 cut(s) 1165
AoxI GGCC 5 cut(s) 590, 632, 867, 979, 1102
ApeKI GCWGC 3 cut(s) 1061, 1193, 1265
Asp700I GAANNNNTTC 1 cut(s) 1005
Asp718I GGTACC 1 cut(s) 564
AspLEI GCGC 3 cut(s) 305, 466, 1019
AspS9I GGNCC 2 cut(s) 590, 1102
AsuC2I CCSGG 4 cut(s) 290, 636, 657, 658
AsuHPI GGTGA 4 cut(s) 802, 1044, 1126, 1342
AvaI CYCGRG 2 cut(s) 587, 656
AxyI CCTNAGG 1 cut(s) 936
BaeI ACNNNNGTAYC 2 cut(s) 1213, 1246
BanI GGYRCC 1 cut(s) 564
BbsI GAAGAC 1 cut(s) 800
Bbv12I GWGCWC 2 cut(s) 556, 804
BbvI GCAGC 3 cut(s) 1073, 1180, 1277
BccI CCATC 2 cut(s) 900, 1063
BceAI ACGGC 2 cut(s) 699, 1238
BciT130I CCWGG 3 cut(s) 871, 998, 1342
BciVI GTATCC 1 cut(s) 465
BclI TGATCA 1 cut(s) 1372
BcnI CCSGG 4 cut(s) 290, 636, 657, 658
BcoDI GTCTC 5 cut(s) 132, 205, 674, 1163, 1199
BcuI ACTAGT 1 cut(s) 1096
BfaI CTAG 3 cut(s) 89, 666, 1097
BfuI GTATCC 1 cut(s) 465
BisI GCNGC 3 cut(s) 1062, 1194, 1266
BlsI GCNGC 3 cut(s) 1063, 1195, 1267
Bme1390I CCNGG 7 cut(s) 290, 636, 657, 658, 871, 998, 1342
BmeT110I CYCGRG 2 cut(s) 587, 656
BmgBI CACGTC 1 cut(s) 546
BmgT120I GGNCC 2 cut(s) 590, 1102
BmiI GGNNCC 1 cut(s) 566
BmrFI CCNGG 7 cut(s) 290, 636, 657, 658, 871, 998, 1342
BmrI ACTGGG 1 cut(s) 493
BmsI GCATC 4 cut(s) 220, 931, 1351, 1357
BmuI ACTGGG 1 cut(s) 493
BoxI GACNNNNGTC 1 cut(s) 114
BpiI GAAGAC 1 cut(s) 800
BplI GAGNNNNNCTC 2 cut(s) 112, 144
BpuMI CCSGG 4 cut(s) 290, 636, 657, 658
BsaBI GATNNNNATC 3 cut(s) 81, 651, 1371
BsaI GGTCTC 1 cut(s) 132
BsaJI CCNNGG 5 cut(s) 288, 526, 586, 635, 656
BsaWI WCCGGW 3 cut(s) 1118, 1165, 1327
Bsc4I CCNNNNNNNGG 2 cut(s) 1111, 1347
Bse1I ACTGG 4 cut(s) 480, 499, 568, 1301
Bse21I CCTNAGG 1 cut(s) 936
Bse3DI GCAATG 1 cut(s) 72
Bse8I GATNNNNATC 3 cut(s) 81, 651, 1371
BseAI TCCGGA 1 cut(s) 1165
BseBI CCWGG 3 cut(s) 871, 998, 1342
BseDI CCNNGG 5 cut(s) 288, 526, 586, 635, 656
BseGI GGATG 3 cut(s) 454, 946, 1356
BseJI GATNNNNATC 3 cut(s) 81, 651, 1371
BseLI CCNNNNNNNGG 2 cut(s) 1111, 1347
BseMI GCAATG 1 cut(s) 72
BseMII CTCAG 5 cut(s) 378, 950, 978, 1011, 1266
BseNI ACTGG 4 cut(s) 480, 499, 568, 1301
BseRI GAGGAG 4 cut(s) 277, 404, 1169, 1217
BseXI GCAGC 3 cut(s) 1073, 1180, 1277
BseYI CCCAGC 2 cut(s) 738, 1187
BsgI GTGCAG 3 cut(s) 568, 910, 1284
BshFI GGCC 5 cut(s) 592, 634, 869, 981, 1104
BshNI GGYRCC 1 cut(s) 564
BsiHKAI GWGCWC 2 cut(s) 556, 804
BsiHKCI CYCGRG 2 cut(s) 587, 656
BsiSI CCGG 6 cut(s) 290, 635, 657, 1119, 1166, 1328
BslFI GGGAC 1 cut(s) 673
BslI CCNNNNNNNGG 2 cut(s) 1111, 1347
BsmAI GTCTC 5 cut(s) 132, 205, 674, 1163, 1199
BsmBI CGTCTC 2 cut(s) 674, 1163
BsmFI GGGAC 1 cut(s) 673
BsnI GGCC 5 cut(s) 592, 634, 869, 981, 1104
Bso31I GGTCTC 1 cut(s) 132
BsoBI CYCGRG 2 cut(s) 587, 656
Bsp1286I GDGCHC 2 cut(s) 556, 804
Bsp13I TCCGGA 1 cut(s) 1165
Bsp143I GATC 4 cut(s) 82, 270, 755, 1372
BspACI CCGC 2 cut(s) 42, 1309
BspANI GGCC 5 cut(s) 592, 634, 869, 981, 1104
BspCNI CTCAG 5 cut(s) 379, 949, 979, 1012, 1267
BspEI TCCGGA 1 cut(s) 1165
BspLI GGNNCC 1 cut(s) 566
BspT107I GGYRCC 1 cut(s) 564
BspTNI GGTCTC 1 cut(s) 132
BsrDI GCAATG 1 cut(s) 72
BsrI ACTGG 4 cut(s) 480, 499, 568, 1301
BssECI CCNNGG 5 cut(s) 288, 526, 586, 635, 656
BssMI GATC 4 cut(s) 82, 270, 755, 1372
BssT1I CCWWGG 1 cut(s) 526
Bst2UI CCWGG 3 cut(s) 871, 998, 1342
Bst4CI ACNGT 4 cut(s) 32, 208, 421, 1038
Bst6I CTCTTC 1 cut(s) 686
BstAPI GCANNNNNTGC 1 cut(s) 901
BstC8I GCNNGC 3 cut(s) 362, 893, 974
BstDEI CTNAG 6 cut(s) 387, 936, 951, 987, 1020, 1275
BstEII GGTNACC 1 cut(s) 1114
BstENI CCTNNNNNAGG 1 cut(s) 1109
BstF5I GGATG 3 cut(s) 454, 946, 1356
BstHHI GCGC 3 cut(s) 305, 466, 1019
BstKTI GATC 4 cut(s) 85, 273, 758, 1375
BstMAI GTCTC 5 cut(s) 132, 205, 674, 1163, 1199
BstMBI GATC 4 cut(s) 82, 270, 755, 1372
BstMWI GCNNNNNNNGC 8 cut(s) 366, 501, 582, 808, 901, 978, 1023, 1193
BstNI CCWGG 3 cut(s) 871, 998, 1342
BstNSI RCATGY 5 cut(s) 237, 830, 934, 1221, 1272
BstPAI GACNNNNGTC 1 cut(s) 114
BstPI GGTNACC 1 cut(s) 1114
BstSCI CCNGG 7 cut(s) 288, 634, 655, 656, 869, 996, 1340
BstV1I GCAGC 3 cut(s) 1073, 1180, 1277
BstV2I GAAGAC 1 cut(s) 800
Bsu36I CCTNAGG 1 cut(s) 936
BsuI GTATCC 1 cut(s) 465
BsuRI GGCC 5 cut(s) 592, 634, 869, 981, 1104
BtgZI GCGATG 2 cut(s) 69, 919
BtrI CACGTC 1 cut(s) 546
BtsCI GGATG 3 cut(s) 454, 946, 1356
BtsI GCAGTG 2 cut(s) 473, 556
BtsIMutI CAGTG 3 cut(s) 473, 506, 556
Cac8I GCNNGC 3 cut(s) 362, 893, 974
CaiI CAGNNNCTG 2 cut(s) 1042, 1067
CfoI GCGC 3 cut(s) 305, 466, 1019
Cfr13I GGNCC 2 cut(s) 590, 1102
Cfr9I CCCGGG 1 cut(s) 656
CsiI ACCWGGT 1 cut(s) 1340
Csp6I GTAC 4 cut(s) 384, 565, 1230, 1314
CspCI CAANNNNNGTGG 2 cut(s) 1080, 1115
CviAII CATG 9 cut(s) 17, 234, 351, 827, 845, 931, 983, 1218, 1269
CviQI GTAC 4 cut(s) 384, 565, 1230, 1314
DdeI CTNAG 6 cut(s) 387, 936, 951, 987, 1020, 1275
DpnI GATC 4 cut(s) 84, 272, 757, 1374
DpnII GATC 4 cut(s) 82, 270, 755, 1372
DraI TTTAAA 1 cut(s) 1087
Eam1104I CTCTTC 1 cut(s) 686
EarI CTCTTC 1 cut(s) 686
Eco130I CCWWGG 1 cut(s) 526
Eco147I AGGCCT 1 cut(s) 869
Eco31I GGTCTC 1 cut(s) 132
Eco57I CTGAAG 1 cut(s) 161
Eco81I CCTNAGG 1 cut(s) 936
Eco88I CYCGRG 2 cut(s) 587, 656
Eco91I GGTNACC 1 cut(s) 1114
EcoNI CCTNNNNNAGG 1 cut(s) 1109
EcoO65I GGTNACC 1 cut(s) 1114
EcoRII CCWGG 3 cut(s) 869, 996, 1340
EcoT14I CCWWGG 1 cut(s) 526
EcoT22I ATGCAT 2 cut(s) 235, 919
ErhI CCWWGG 1 cut(s) 526
Esp3I CGTCTC 2 cut(s) 674, 1163
FaeI CATG 9 cut(s) 20, 237, 354, 830, 848, 934, 986, 1221, 1272
FaqI GGGAC 1 cut(s) 673
FatI CATG 9 cut(s) 16, 233, 350, 826, 844, 930, 982, 1217, 1268
FauNDI CATATG 2 cut(s) 340, 919
FbaI TGATCA 1 cut(s) 1372
Fnu4HI GCNGC 3 cut(s) 1062, 1194, 1266
FokI GGATG 3 cut(s) 461, 953, 1363
Fsp4HI GCNGC 3 cut(s) 1062, 1194, 1266
FspBI CTAG 3 cut(s) 89, 666, 1097
FspI TGCGCA 2 cut(s) 304, 465
GlaI GCGC 3 cut(s) 304, 465, 1018
GluI GCNGC 3 cut(s) 1062, 1194, 1266
GsaI CCCAGC 2 cut(s) 742, 1191
HaeIII GGCC 5 cut(s) 592, 634, 869, 981, 1104
HapII CCGG 6 cut(s) 290, 635, 657, 1119, 1166, 1328
HhaI GCGC 3 cut(s) 305, 466, 1019
Hin1II CATG 9 cut(s) 20, 237, 354, 830, 848, 934, 986, 1221, 1272
Hin6I GCGC 3 cut(s) 303, 464, 1017
HinP1I GCGC 3 cut(s) 303, 464, 1017
HinfI GANTC 3 cut(s) 38, 394, 452
HpaII CCGG 6 cut(s) 290, 635, 657, 1119, 1166, 1328
HphI GGTGA 4 cut(s) 802, 1044, 1126, 1342
Hpy166II GTNNAC 2 cut(s) 239, 832
Hpy188I TCNGA 4 cut(s) 120, 229, 1069, 1276
Hpy188III TCNNGA 3 cut(s) 179, 938, 1166
Hpy8I GTNNAC 2 cut(s) 239, 832
HpyCH4III ACNGT 4 cut(s) 32, 208, 421, 1038
HpyCH4IV ACGT 1 cut(s) 545
HpyF10VI GCNNNNNNNGC 8 cut(s) 366, 501, 582, 808, 901, 978, 1023, 1193
HpyF3I CTNAG 6 cut(s) 387, 936, 951, 987, 1020, 1275
HpySE526I ACGT 1 cut(s) 545
Hsp92II CATG 9 cut(s) 20, 237, 354, 830, 848, 934, 986, 1221, 1272
HspAI GCGC 3 cut(s) 303, 464, 1017
Kpn2I TCCGGA 1 cut(s) 1165
KpnI GGTACC 1 cut(s) 568
Ksp22I TGATCA 1 cut(s) 1372
Kzo9I GATC 4 cut(s) 82, 270, 755, 1372
LmnI GCTCC 3 cut(s) 249, 559, 1184
Lsp1109I GCAGC 3 cut(s) 1073, 1180, 1277
LweI GCATC 4 cut(s) 220, 931, 1351, 1357
MabI ACCWGGT 1 cut(s) 1340
MaeI CTAG 3 cut(s) 89, 666, 1097
MaeII ACGT 1 cut(s) 545
MaeIII GTNAC 8 cut(s) 296, 1114, 1141, 1220, 1255, 1292, 1304, 1330
MalI GATC 4 cut(s) 84, 272, 757, 1374
MboI GATC 4 cut(s) 82, 270, 755, 1372
MfeI CAATTG 2 cut(s) 513, 1012
MhlI GDGCHC 2 cut(s) 556, 804
MmeI TCCRAC 1 cut(s) 1328
Mph1103I ATGCAT 2 cut(s) 235, 919
MroI TCCGGA 1 cut(s) 1165
MroXI GAANNNNTTC 1 cut(s) 1005
MseI TTAA 3 cut(s) 480, 807, 1086
MslI CAYNNNNRTG 2 cut(s) 21, 729
MspA1I CMGCKG 1 cut(s) 1311
MspI CCGG 6 cut(s) 290, 635, 657, 1119, 1166, 1328
MspR9I CCNGG 7 cut(s) 290, 636, 657, 658, 871, 998, 1342
MunI CAATTG 2 cut(s) 513, 1012
MvaI CCWGG 3 cut(s) 871, 998, 1342
MwoI GCNNNNNNNGC 8 cut(s) 366, 501, 582, 808, 901, 978, 1023, 1193
NciI CCSGG 4 cut(s) 290, 636, 657, 658
NdeI CATATG 2 cut(s) 340, 919
NdeII GATC 4 cut(s) 82, 270, 755, 1372
NlaIII CATG 9 cut(s) 20, 237, 354, 830, 848, 934, 986, 1221, 1272
NlaIV GGNNCC 1 cut(s) 566
NmuCI GTSAC 4 cut(s) 1114, 1220, 1304, 1330
NsbI TGCGCA 2 cut(s) 304, 465
NsiI ATGCAT 2 cut(s) 235, 919
NspI RCATGY 5 cut(s) 237, 830, 934, 1221, 1272
PceI AGGCCT 1 cut(s) 869
PciI ACATGT 2 cut(s) 826, 1217
PdmI GAANNNNTTC 1 cut(s) 1005
PfeI GAWTC 3 cut(s) 38, 394, 452
PflMI CCANNNNNTGG 1 cut(s) 1347
PkrI GCNGC 3 cut(s) 1063, 1195, 1267
PscI ACATGT 2 cut(s) 826, 1217
PshAI GACNNNNGTC 1 cut(s) 114
Psp6I CCWGG 3 cut(s) 869, 996, 1340
PspEI GGTNACC 1 cut(s) 1114
PspFI CCCAGC 2 cut(s) 738, 1187
PspGI CCWGG 3 cut(s) 869, 996, 1340
PspN4I GGNNCC 1 cut(s) 566
PspPI GGNCC 2 cut(s) 590, 1102
PstNI CAGNNNCTG 2 cut(s) 1042, 1067
RsaI GTAC 4 cut(s) 385, 566, 1231, 1315
RsaNI GTAC 4 cut(s) 384, 565, 1230, 1314
RseI CAYNNNNRTG 2 cut(s) 21, 729
SaqAI TTAA 3 cut(s) 480, 807, 1086
SatI GCNGC 3 cut(s) 1062, 1194, 1266
Sau3AI GATC 4 cut(s) 82, 270, 755, 1372
Sau96I GGNCC 2 cut(s) 590, 1102
ScrFI CCNGG 7 cut(s) 290, 636, 657, 658, 871, 998, 1342
SduI GDGCHC 2 cut(s) 556, 804
SexAI ACCWGGT 1 cut(s) 1340
SfaNI GCATC 4 cut(s) 220, 931, 1351, 1357
SmaI CCCGGG 1 cut(s) 658
SmiMI CAYNNNNRTG 2 cut(s) 21, 729
SpeI ACTAGT 1 cut(s) 1096
SseBI AGGCCT 1 cut(s) 869
SsiI CCGC 2 cut(s) 42, 1309
SspMI CTAG 3 cut(s) 89, 666, 1097
StuI AGGCCT 1 cut(s) 869
StyD4I CCNGG 7 cut(s) 288, 634, 655, 656, 869, 996, 1340
StyI CCWWGG 1 cut(s) 526
TaaI ACNGT 4 cut(s) 32, 208, 421, 1038
TaiI ACGT 1 cut(s) 548
TaqI TCGA 2 cut(s) 105, 966
TaqII GACCGA 1 cut(s) 812
TatI WGTACW 1 cut(s) 383
TfiI GAWTC 3 cut(s) 38, 394, 452
Tru1I TTAA 3 cut(s) 480, 807, 1086
Tru9I TTAA 3 cut(s) 480, 807, 1086
TscAI CASTG 3 cut(s) 473, 506, 556
TseFI GTSAC 4 cut(s) 1114, 1220, 1304, 1330
TseI GCWGC 3 cut(s) 1061, 1193, 1265
Tsp45I GTSAC 4 cut(s) 1114, 1220, 1304, 1330
TspDTI ATGAA 8 cut(s) 17, 51, 72, 83, 339, 689, 713, 747
TspMI CCCGGG 1 cut(s) 656
TspRI CASTG 3 cut(s) 473, 506, 556
Van91I CCANNNNNTGG 1 cut(s) 1347
XagI CCTNNNNNAGG 1 cut(s) 1109
XceI RCATGY 5 cut(s) 237, 830, 934, 1221, 1272
XcmI CCANNNNNNNNNTGG 1 cut(s) 1293
XmaI CCCGGG 1 cut(s) 656
XmnI GAANNNNTTC 1 cut(s) 1005
XspI CTAG 3 cut(s) 89, 666, 1097
Zsp2I ATGCAT 2 cut(s) 235, 919
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.