Rorug06G0294600

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000864
Physical Location & Seq
Reverse (-)
44677742 .. 44680191
2450 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0294600.1

Sequence Viewer

Length: 1440 bp
ATGGGCTTCATGATGGAGGAGCATAATCAGAAGAATTTTGAAGAGTCCAACTCAGGACCAAAAGGGGTACATTTGGTGGATTCGGCTAATTTGGATGTCACATGTTTAGAAACAGAAATTGGGAGGTTGACATACACAGTTGATTGCATGGAACCCAAGCTGGAGGATTTAAGATTACATGTATTTAACTTAGAACATGCGATGCTTGAAATATTGAATGGTGATTGTATTGAGGAAATTGTTTCTGAGGTTGTCGAAATTGTGAGTTTAAAGGCCAATAAGACTCATAACCTGTTGAGTCGAACTGTTGGTACAACCTTTGTGGAGCAAATGGATGAGGATTTGTTTGGTGAACATGGGAGAAGCAGTCAAGACCCACTAAAAAAATTGACCAAGTCACCAGTGCTAGATGGCTATGCTGATGAGGGACCCCATGAGGAACTCATACATGATGAGCACAAACCTGACTCCGTTACGAGGGTTAAACAGCAGCCACAAAAGACTCTTCGTTCTCGAAAGCAACAAAGGAGTAAACCCAAAGCCCCAATTTTTGCAATAAGCGGTCTCATCACACCTGAGAATGCACATCTCATGAAATTTTTATTTAGGAAGAATGGAATTGGAGAAGACAAGTGGAGTGAAGTAGTAGCTAGTTATGGTGACTACGCCGTTACTAGAAAGGAATTTCAGTGTCTCTCTCCAGCAACTCCACTATCTTGCAAGGTCATAAACATATCTGCGGCGTATTTGAATGAGCCGGAGTCAGAACATTGGTTTCTCCCTTCCTTTTTTGGGGAACGAGCGAGGGTAAAAGATGAATCATGTAGCTATGCTAAGTGGCTCACATCAACCATTGAGATGTGTGGTCTTAAGAGGTTTCATCGCCGTCTCCAACAATGCTCCAAGATATTCATTCCCTTGCACGAAAATATGGAAGAGCACTGGATTCTGTTGGTCATGCACCTCCCTGAAAAAAAGGCAGAGGTGCTGGACAGCTTCCCTGATGTAAGGTCACGTGAGAGGCGAATGGATCATGCAAGAGACGTGATGGCAATGTTGCAGAAGGTATTTGGTACTGAAACAACATGGTCAAACGCCAAATTTTATCATTTTCCATCATTCACTCTTAACTTTTCGGACTTTAACCCAACGCATGAACAAAAATCTGAGAGCGGGGTATTTGTTATAAGGCACATGCAGTACTACCGTGGCATGTGGTCAACAACGTTTCATTCGGAGGACCAGCGTACTCGATTGGCACTTGAAATTGTTCTTCATCCAAGGAATGAACACCGTCAAATATTGATTGGAGCAGCAGCAGGAGCTTCGATGCCGCCAATAGGTGAAAGTGAGCTATTCAAAAATATTCTTCTTCCACCAACCGGAAGAAAGTTTCGTAGCCACCGTAAGCGGAGAGATCATGTGTCGAAGGACGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IBR zf-RanBP

Protein Analysis

479

Amino Acids

55.26

Weight (kDa)

6.57

Isoelectric Point (pI)

54.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C48 PF02902 289 - 403 7.4e-10 Ulp1 protease family, C-terminal catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1187
AatII GACGTC 1 cut(s) 1437
AccBSI CCGCTC 1 cut(s) 1173
AciI CCGC 5 cut(s) 561, 740, 1173, 1334, 1411
AclI AACGTT 1 cut(s) 1226
AclWI GGATC 1 cut(s) 1038
AcsI RAATTY 4 cut(s) 34, 596, 683, 1100
AcvI CACGTG 1 cut(s) 1016
AcyI GRCGYC 1 cut(s) 1434
AfaI GTAC 5 cut(s) 69, 313, 1075, 1202, 1249
AfiI CCNNNNNNNGG 4 cut(s) 477, 792, 1340, 1382
AflII CTTAAG 1 cut(s) 869
AflIII ACRYGT 2 cut(s) 101, 178
AgsI TTSAA 6 cut(s) 41, 209, 217, 751, 1265, 1360
AjiI CACGTC 1 cut(s) 1045
AjuI GAANNNNNNNTTGG 2 cut(s) 102, 134
AluBI AGCT 6 cut(s) 160, 650, 828, 996, 1325, 1354
AluI AGCT 6 cut(s) 160, 650, 828, 996, 1325, 1354
Alw21I GWGCWC 2 cut(s) 459, 942
Alw26I GTCTC 4 cut(s) 569, 698, 893, 1035
AlwI GGATC 1 cut(s) 1038
AoxI GGCC 1 cut(s) 273
ApeKI GCWGC 3 cut(s) 490, 1313, 1316
ApoI RAATTY 4 cut(s) 34, 596, 683, 1100
Asp700I GAANNNNTTC 1 cut(s) 1269
AspS9I GGNCC 3 cut(s) 56, 428, 1240
AsuHPI GGTGA 5 cut(s) 233, 362, 390, 671, 1355
AvaII GGWCC 3 cut(s) 56, 428, 1240
BbrPI CACGTG 1 cut(s) 1016
BbsI GAAGAC 1 cut(s) 633
Bbv12I GWGCWC 2 cut(s) 459, 942
BbvI GCAGC 3 cut(s) 502, 1325, 1328
BccI CCATC 4 cut(s) 7, 404, 1042, 1123
BceAI ACGGC 2 cut(s) 653, 870
BcgI CGANNNNNNTGC 2 cut(s) 1308, 1342
BcoDI GTCTC 4 cut(s) 569, 698, 893, 1035
BfaI CTAG 4 cut(s) 407, 651, 675, 1438
BfrI CTTAAG 1 cut(s) 869
BisI GCNGC 5 cut(s) 491, 741, 1314, 1317, 1334
BlsI GCNGC 5 cut(s) 492, 742, 1315, 1318, 1335
BmcAI AGTACT 1 cut(s) 1202
Bme18I GGWCC 3 cut(s) 56, 428, 1240
BmgBI CACGTC 1 cut(s) 1045
BmgT120I GGNCC 3 cut(s) 56, 428, 1240
BmiI GGNNCC 3 cut(s) 153, 429, 430
BmsI GCATC 2 cut(s) 192, 1320
BpiI GAAGAC 1 cut(s) 633
BplI GAGNNNNNCTC 2 cut(s) 35, 67
BpmI CTGGAG 2 cut(s) 182, 684
BsaAI YACGTR 1 cut(s) 1016
BsaHI GRCGYC 1 cut(s) 1434
BsaI GGTCTC 1 cut(s) 569
BsaJI CCNNGG 2 cut(s) 1207, 1280
BsaWI WCCGGW 1 cut(s) 1382
BsaXI ACNNNNNCTCC 2 cut(s) 352, 382
Bsc4I CCNNNNNNNGG 4 cut(s) 477, 792, 1340, 1382
Bse1I ACTGG 2 cut(s) 401, 947
Bse3DI GCAATG 1 cut(s) 1059
BseDI CCNNGG 2 cut(s) 1207, 1280
BseGI GGATG 3 cut(s) 100, 340, 1276
BseLI CCNNNNNNNGG 4 cut(s) 477, 792, 1340, 1382
BseMI GCAATG 1 cut(s) 1059
BseMII CTCAG 4 cut(s) 66, 237, 567, 1158
BseNI ACTGG 2 cut(s) 401, 947
BseRI GAGGAG 1 cut(s) 32
BseXI GCAGC 3 cut(s) 502, 1325, 1328
BshFI GGCC 1 cut(s) 275
BsiHKAI GWGCWC 2 cut(s) 459, 942
BsiSI CCGG 2 cut(s) 758, 1383
BslFI GGGAC 1 cut(s) 441
BslI CCNNNNNNNGG 4 cut(s) 477, 792, 1340, 1382
BsmAI GTCTC 4 cut(s) 569, 698, 893, 1035
BsmBI CGTCTC 2 cut(s) 893, 1035
BsmFI GGGAC 1 cut(s) 441
BsmI GAATGC 1 cut(s) 586
BsnI GGCC 1 cut(s) 275
Bso31I GGTCTC 1 cut(s) 569
Bsp1286I GDGCHC 2 cut(s) 459, 942
Bsp143I GATC 2 cut(s) 1030, 1417
BspACI CCGC 5 cut(s) 561, 740, 1173, 1334, 1411
BspANI GGCC 1 cut(s) 275
BspCNI CTCAG 4 cut(s) 65, 238, 568, 1159
BspHI TCATGA 2 cut(s) 9, 591
BspLI GGNNCC 3 cut(s) 153, 429, 430
BspPI GGATC 1 cut(s) 1038
BspQI GCTCTTC 1 cut(s) 930
BspTI CTTAAG 1 cut(s) 869
BspTNI GGTCTC 1 cut(s) 569
BsrBI CCGCTC 1 cut(s) 1173
BsrDI GCAATG 1 cut(s) 1059
BsrI ACTGG 2 cut(s) 401, 947
BssECI CCNNGG 2 cut(s) 1207, 1280
BssMI GATC 2 cut(s) 1030, 1417
BssNI GRCGYC 1 cut(s) 1434
BssT1I CCWWGG 1 cut(s) 1280
Bst4CI ACNGT 5 cut(s) 139, 307, 1208, 1295, 1406
Bst6I CTCTTC 3 cut(s) 36, 510, 930
BstACI GRCGYC 1 cut(s) 1434
BstAFI CTTAAG 1 cut(s) 869
BstBAI YACGTR 1 cut(s) 1016
BstDEI CTNAG 6 cut(s) 52, 190, 246, 576, 834, 1167
BstDSI CCRYGG 1 cut(s) 1207
BstF5I GGATG 3 cut(s) 100, 340, 1276
BstKTI GATC 2 cut(s) 1033, 1420
BstMAI GTCTC 4 cut(s) 569, 698, 893, 1035
BstMBI GATC 2 cut(s) 1030, 1417
BstMWI GCNNNNNNNGC 1 cut(s) 1322
BstNSI RCATGY 5 cut(s) 105, 182, 200, 1198, 1216
BstV1I GCAGC 3 cut(s) 502, 1325, 1328
BstV2I GAAGAC 1 cut(s) 633
BsuRI GGCC 1 cut(s) 275
BtgI CCRYGG 1 cut(s) 1207
BtgZI GCGATG 2 cut(s) 215, 866
BtrI CACGTC 1 cut(s) 1045
BtsCI GGATG 3 cut(s) 100, 340, 1276
BtsIMutI CAGTG 3 cut(s) 408, 695, 940
CciI TCATGA 2 cut(s) 9, 591
Cfr13I GGNCC 3 cut(s) 56, 428, 1240
Csp6I GTAC 5 cut(s) 68, 312, 1074, 1201, 1248
CspCI CAANNNNNGTGG 2 cut(s) 303, 338
CviQI GTAC 5 cut(s) 68, 312, 1074, 1201, 1248
DdeI CTNAG 6 cut(s) 52, 190, 246, 576, 834, 1167
DpnI GATC 2 cut(s) 1032, 1419
DpnII GATC 2 cut(s) 1030, 1417
DraI TTTAAA 1 cut(s) 270
Eam1104I CTCTTC 3 cut(s) 36, 510, 930
EarI CTCTTC 3 cut(s) 36, 510, 930
Eco130I CCWWGG 1 cut(s) 1280
Eco31I GGTCTC 1 cut(s) 569
Eco47I GGWCC 3 cut(s) 56, 428, 1240
Eco72I CACGTG 1 cut(s) 1016
EcoO109I RGGNCCY 1 cut(s) 428
EcoT14I CCWWGG 1 cut(s) 1280
ErhI CCWWGG 1 cut(s) 1280
Esp3I CGTCTC 2 cut(s) 893, 1035
FaqI GGGAC 1 cut(s) 441
FauI CCCGC 1 cut(s) 1166
Fnu4HI GCNGC 5 cut(s) 491, 741, 1314, 1317, 1334
FokI GGATG 3 cut(s) 107, 347, 1263
Fsp4HI GCNGC 5 cut(s) 491, 741, 1314, 1317, 1334
FspBI CTAG 4 cut(s) 407, 651, 675, 1438
GluI GCNGC 5 cut(s) 491, 741, 1314, 1317, 1334
GsuI CTGGAG 2 cut(s) 182, 684
HaeIII GGCC 1 cut(s) 275
HapII CCGG 2 cut(s) 758, 1383
Hin1I GRCGYC 1 cut(s) 1434
HincII GTYRAC 2 cut(s) 129, 1221
HindII GTYRAC 2 cut(s) 129, 1221
HinfI GANTC 9 cut(s) 44, 80, 283, 298, 467, 502, 761, 818, 946
HpaII CCGG 2 cut(s) 758, 1383
HphI GGTGA 5 cut(s) 233, 362, 390, 671, 1355
Hpy166II GTNNAC 4 cut(s) 129, 353, 533, 1221
Hpy188I TCNGA 6 cut(s) 30, 247, 766, 1138, 1168, 1237
Hpy188III TCNNGA 5 cut(s) 10, 54, 371, 513, 592
Hpy8I GTNNAC 4 cut(s) 129, 353, 533, 1221
HpyAV CCTTC 3 cut(s) 792, 1057, 1423
HpyCH4III ACNGT 5 cut(s) 139, 307, 1208, 1295, 1406
HpyCH4IV ACGT 4 cut(s) 1015, 1044, 1226, 1434
HpyCH4V TGCA 9 cut(s) 147, 554, 584, 720, 922, 961, 1037, 1060, 1198
HpyF10VI GCNNNNNNNGC 1 cut(s) 1322
HpyF3I CTNAG 6 cut(s) 52, 190, 246, 576, 834, 1167
HpySE526I ACGT 4 cut(s) 1015, 1044, 1226, 1434
Hsp92I GRCGYC 1 cut(s) 1434
KflI GGGWCCC 1 cut(s) 428
Kzo9I GATC 2 cut(s) 1030, 1417
LguI GCTCTTC 1 cut(s) 930
LmnI GCTCC 5 cut(s) 19, 325, 905, 1310, 1322
Lsp1109I GCAGC 3 cut(s) 502, 1325, 1328
LweI GCATC 2 cut(s) 192, 1320
MaeI CTAG 4 cut(s) 407, 651, 675, 1438
MaeII ACGT 4 cut(s) 1015, 1044, 1226, 1434
MaeIII GTNAC 6 cut(s) 97, 396, 472, 659, 670, 1011
MalI GATC 2 cut(s) 1032, 1419
MbiI CCGCTC 1 cut(s) 1173
MboI GATC 2 cut(s) 1030, 1417
MhlI GDGCHC 2 cut(s) 459, 942
MlyI GAGTC 6 cut(s) 53, 277, 307, 461, 496, 770
MmeI TCCRAC 2 cut(s) 72, 916
MroXI GAANNNNTTC 1 cut(s) 1269
MseI TTAA 7 cut(s) 170, 186, 269, 483, 870, 1128, 1143
MslI CAYNNNNRTG 1 cut(s) 857
MspCI CTTAAG 1 cut(s) 869
MspI CCGG 2 cut(s) 758, 1383
Mva1269I GAATGC 1 cut(s) 586
MwoI GCNNNNNNNGC 1 cut(s) 1322
NdeII GATC 2 cut(s) 1030, 1417
NlaIV GGNNCC 3 cut(s) 153, 429, 430
NmuCI GTSAC 4 cut(s) 97, 396, 659, 1011
NspI RCATGY 5 cut(s) 105, 182, 200, 1198, 1216
PagI TCATGA 2 cut(s) 9, 591
PciI ACATGT 2 cut(s) 101, 178
PciSI GCTCTTC 1 cut(s) 930
PcsI WCGNNNNNNNCGW 2 cut(s) 1021, 1402
PctI GAATGC 1 cut(s) 586
PdmI GAANNNNTTC 1 cut(s) 1269
PfeI GAWTC 3 cut(s) 80, 818, 946
PflFI GACNNNGTC 1 cut(s) 394
PkrI GCNGC 5 cut(s) 492, 742, 1315, 1318, 1335
PleI GAGTC 6 cut(s) 52, 277, 306, 461, 496, 769
PmaCI CACGTG 1 cut(s) 1016
PmlI CACGTG 1 cut(s) 1016
PpsI GAGTC 6 cut(s) 52, 277, 306, 461, 496, 769
Ppu21I YACGTR 1 cut(s) 1016
PpuMI RGGWCCY 1 cut(s) 428
PscI ACATGT 2 cut(s) 101, 178
PsiI TTATAA 1 cut(s) 1187
Psp1406I AACGTT 1 cut(s) 1226
Psp5II RGGWCCY 1 cut(s) 428
PspCI CACGTG 1 cut(s) 1016
PspN4I GGNNCC 3 cut(s) 153, 429, 430
PspPI GGNCC 3 cut(s) 56, 428, 1240
PspPPI RGGWCCY 1 cut(s) 428
PsrI GAACNNNNNNTAC 2 cut(s) 295, 327
PsyI GACNNNGTC 1 cut(s) 394
RsaI GTAC 5 cut(s) 69, 313, 1075, 1202, 1249
RsaNI GTAC 5 cut(s) 68, 312, 1074, 1201, 1248
RseI CAYNNNNRTG 1 cut(s) 857
SapI GCTCTTC 1 cut(s) 930
SaqAI TTAA 7 cut(s) 170, 186, 269, 483, 870, 1128, 1143
SatI GCNGC 5 cut(s) 491, 741, 1314, 1317, 1334
Sau3AI GATC 2 cut(s) 1030, 1417
Sau96I GGNCC 3 cut(s) 56, 428, 1240
ScaI AGTACT 1 cut(s) 1202
SchI GAGTC 6 cut(s) 53, 277, 307, 461, 496, 770
SduI GDGCHC 2 cut(s) 459, 942
SfaNI GCATC 2 cut(s) 192, 1320
SinI GGWCC 3 cut(s) 56, 428, 1240
SmiMI CAYNNNNRTG 1 cut(s) 857
SmlI CTYRAG 1 cut(s) 869
SmoI CTYRAG 1 cut(s) 869
SsiI CCGC 5 cut(s) 561, 740, 1173, 1334, 1411
SspI AATATT 3 cut(s) 213, 1302, 1366
SspMI CTAG 4 cut(s) 407, 651, 675, 1438
StyI CCWWGG 1 cut(s) 1280
TaaI ACNGT 5 cut(s) 139, 307, 1208, 1295, 1406
TaiI ACGT 4 cut(s) 1018, 1047, 1229, 1437
TaqI TCGA 6 cut(s) 255, 301, 514, 1252, 1328, 1427
TatI WGTACW 1 cut(s) 1200
TauI GCSGC 2 cut(s) 743, 1336
TfiI GAWTC 3 cut(s) 80, 818, 946
Tru1I TTAA 7 cut(s) 170, 186, 269, 483, 870, 1128, 1143
Tru9I TTAA 7 cut(s) 170, 186, 269, 483, 870, 1128, 1143
TscAI CASTG 3 cut(s) 408, 695, 947
TseFI GTSAC 4 cut(s) 97, 396, 659, 1011
TseI GCWGC 3 cut(s) 490, 1313, 1316
Tsp45I GTSAC 4 cut(s) 97, 396, 659, 1011
TspDTI ATGAA 8 cut(s) 608, 831, 869, 901, 1170, 1220, 1265, 1302
TspGWI ACGGA 1 cut(s) 460
TspRI CASTG 3 cut(s) 408, 695, 947
Tth111I GACNNNGTC 1 cut(s) 394
Vha464I CTTAAG 1 cut(s) 869
VpaK11BI GGWCC 3 cut(s) 56, 428, 1240
XapI RAATTY 4 cut(s) 34, 596, 683, 1100
XceI RCATGY 5 cut(s) 105, 182, 200, 1198, 1216
XmnI GAANNNNTTC 1 cut(s) 1269
XspI CTAG 4 cut(s) 407, 651, 675, 1438
ZraI GACGTC 1 cut(s) 1435
ZrmI AGTACT 1 cut(s) 1202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.