Rmu_co8404089.1_g000001

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8404089.1
OG0000864
Physical Location & Seq
Reverse (-)
188 .. 1261
1074 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8404089.1_g000001.1.cds

Sequence Viewer

Length: 1074 bp
atgctgagatgtcctgaaccatcttgtaaggcagcagttggtcctgaattaatcgaaaacgtgctggtggtgacaggaaaaaaagcctggtatgacaagtacaagcattacctgttaagatcatatgtcgaacataacaggaagaagattagatggtgtcctgctccggattgcaagtatgctattaagtttgatgctccagacagaatgtcatcatatgatgtttcgtgtctttgctcgcatagttattgctggaattgtgaggaggagaatcatcgtccggtgttctgcgagactgtgaagaagtggattttgaagaatgcggatgattctcagaatggacaatggataatggttaataccaagccctgtcctaagtgtaacagatcgatcgaaaagaaccaagggtgtaatcacatgacctgcacagctccttgtagatttcaattttgctggtattgcctcggaccatacggtacatgccattgcaacgcgtttaataggagggcaactgatatgtcaggggaagtttggggcatggctgagaaagagagaatgcaggcaaagatagatttacagagatatgatcattactatgaacgttgggcaaacaatggatcatcaaaggaaagagctctcaaggattttgataaggtgaagaccgtgcacattaaggaacttggacacatacagaaaaatacaatagagcataagtacgattttatcatagaggcctggcaacagataattgaatgcaggcaggtattgcgatggacctatgcttatggatactacatgaaggagaatgaggatgctaagcgtcatctcttcgagcacttgcaaggccatgccgagttcagcctggagaggcttcacaaatgtgcagagagtgaactgcaacagtttctcaacctggctcatgacgataaggtttggttcatagagttccaaaagaaactcgtgagtctgacaaaggtgactggcacttatttcgacaggctggttactgcgttggaggacggcctttctgagattttaacgaagaaacagaaaaagaaagttcttcgaaattag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IBR zf-RanBP

Protein Analysis

357

Amino Acids

42.29

Weight (kDa)

8.69

Isoelectric Point (pI)

61.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 431, 751
AccII CGCG 1 cut(s) 494
AccIII TCCGGA 1 cut(s) 166
AciI CCGC 1 cut(s) 323
AclI AACGTT 1 cut(s) 601
AclWI GGATC 1 cut(s) 625
AfaI GTAC 3 cut(s) 101, 478, 716
AflIII ACRYGT 1 cut(s) 492
AgsI TTSAA 3 cut(s) 316, 446, 752
AhdI GACNNNNNGTC 1 cut(s) 208
AjnI CCWGG 4 cut(s) 86, 734, 861, 912
AleI CACNNNNGTG 1 cut(s) 879
AluBI AGCT 2 cut(s) 431, 635
AluI AGCT 2 cut(s) 431, 635
Alw21I GWGCWC 3 cut(s) 637, 669, 837
Alw26I GTCTC 1 cut(s) 287
Alw44I GTGCAC 1 cut(s) 665
AlwI GGATC 1 cut(s) 625
Aor13HI TCCGGA 1 cut(s) 166
AoxI GGCC 3 cut(s) 732, 844, 1021
ApaLI GTGCAC 1 cut(s) 665
ApeKI GCWGC 1 cut(s) 32
AseI ATTAAT 1 cut(s) 50
AspS9I GGNCC 3 cut(s) 41, 467, 774
AsuHPI GGTGA 3 cut(s) 82, 667, 988
AsuII TTCGAA 1 cut(s) 1066
AvaII GGWCC 3 cut(s) 41, 467, 774
BaeGI GKGCMC 1 cut(s) 669
BanII GRGCYC 1 cut(s) 637
BauI CACGAG 1 cut(s) 959
BbsI GAAGAC 1 cut(s) 665
Bbv12I GWGCWC 3 cut(s) 637, 669, 837
BbvI GCAGC 1 cut(s) 44
BccI CCATC 3 cut(s) 28, 147, 765
BceAI ACGGC 1 cut(s) 1036
BcgI CGANNNNNNTGC 2 cut(s) 973, 1007
BciT130I CCWGG 4 cut(s) 88, 736, 863, 914
BciVI GTATCC 1 cut(s) 782
BclI TGATCA 1 cut(s) 586
BcoDI GTCTC 1 cut(s) 287
BfuAI ACCTGC 2 cut(s) 431, 751
BfuI GTATCC 1 cut(s) 782
BisI GCNGC 1 cut(s) 33
BlpI GCTNAGC 1 cut(s) 816
BlsI GCNGC 1 cut(s) 34
Bme1390I CCNGG 4 cut(s) 88, 736, 863, 914
Bme18I GGWCC 3 cut(s) 41, 467, 774
BmeRI GACNNNNNGTC 1 cut(s) 208
BmgT120I GGNCC 3 cut(s) 41, 467, 774
BmrFI CCNGG 4 cut(s) 88, 736, 863, 914
BmsI GCATC 2 cut(s) 184, 802
BpiI GAAGAC 1 cut(s) 665
BpmI CTGGAG 2 cut(s) 183, 884
Bpu1102I GCTNAGC 1 cut(s) 816
Bpu14I TTCGAA 1 cut(s) 1066
BpuEI CTTGAG 1 cut(s) 623
Bsa29I ATCGAT 1 cut(s) 389
BsaJI CCNNGG 2 cut(s) 403, 463
BsaWI WCCGGW 2 cut(s) 166, 280
Bse1I ACTGG 1 cut(s) 985
Bse3DI GCAATG 1 cut(s) 484
BseAI TCCGGA 1 cut(s) 166
BseBI CCWGG 4 cut(s) 88, 736, 863, 914
BseCI ATCGAT 1 cut(s) 389
BseDI CCNNGG 2 cut(s) 403, 463
BseGI GGATG 2 cut(s) 331, 817
BseMI GCAATG 1 cut(s) 484
BseMII CTCAG 3 cut(s) 347, 534, 1020
BseNI ACTGG 1 cut(s) 985
BseRI GAGGAG 2 cut(s) 278, 281
BseSI GKGCMC 1 cut(s) 669
BseXI GCAGC 1 cut(s) 44
BsgI GTGCAG 2 cut(s) 409, 903
Bsh1236I CGCG 1 cut(s) 494
Bsh1285I CGRYCG 1 cut(s) 393
BshFI GGCC 3 cut(s) 734, 846, 1023
BshVI ATCGAT 1 cut(s) 389
BsiEI CGRYCG 1 cut(s) 393
BsiHKAI GWGCWC 3 cut(s) 637, 669, 837
BsiSI CCGG 2 cut(s) 167, 281
BsmAI GTCTC 1 cut(s) 287
BsmI GAATGC 3 cut(s) 325, 561, 758
BsnI GGCC 3 cut(s) 734, 846, 1023
Bsp119I TTCGAA 1 cut(s) 1066
Bsp1286I GDGCHC 3 cut(s) 637, 669, 837
Bsp13I TCCGGA 1 cut(s) 166
Bsp143I GATC 5 cut(s) 119, 386, 390, 586, 617
Bsp1720I GCTNAGC 1 cut(s) 816
BspACI CCGC 1 cut(s) 323
BspANI GGCC 3 cut(s) 734, 846, 1023
BspCNI CTCAG 3 cut(s) 346, 535, 1021
BspDI ATCGAT 1 cut(s) 389
BspEI TCCGGA 1 cut(s) 166
BspFNI CGCG 1 cut(s) 494
BspHI TCATGA 1 cut(s) 919
BspMI ACCTGC 2 cut(s) 431, 751
BspPI GGATC 1 cut(s) 625
BspT104I TTCGAA 1 cut(s) 1066
BsrDI GCAATG 1 cut(s) 484
BsrI ACTGG 1 cut(s) 985
BssECI CCNNGG 2 cut(s) 403, 463
BssMI GATC 5 cut(s) 119, 386, 390, 586, 617
BssSI CACGAG 1 cut(s) 959
BssT1I CCWWGG 1 cut(s) 403
Bst2BI CACGAG 1 cut(s) 959
Bst2UI CCWGG 4 cut(s) 88, 736, 863, 914
Bst4CI ACNGT 4 cut(s) 298, 476, 664, 903
Bst6I CTCTTC 1 cut(s) 833
BstAPI GCANNNNNTGC 1 cut(s) 766
BstBI TTCGAA 1 cut(s) 1066
BstC8I GCNNGC 3 cut(s) 239, 561, 758
BstDEI CTNAG 6 cut(s) 5, 333, 375, 543, 816, 1029
BstF5I GGATG 2 cut(s) 331, 817
BstFNI CGCG 1 cut(s) 494
BstKTI GATC 5 cut(s) 122, 389, 393, 589, 620
BstMAI GTCTC 1 cut(s) 287
BstMBI GATC 5 cut(s) 119, 386, 390, 586, 617
BstMCI CGRYCG 1 cut(s) 393
BstMWI GCNNNNNNNGC 2 cut(s) 459, 766
BstNI CCWGG 4 cut(s) 88, 736, 863, 914
BstNSI RCATGY 1 cut(s) 483
BstSCI CCNGG 4 cut(s) 86, 734, 861, 912
BstSLI GKGCMC 1 cut(s) 669
BstUI CGCG 1 cut(s) 494
BstV1I GCAGC 1 cut(s) 44
BstV2I GAAGAC 1 cut(s) 665
Bsu15I ATCGAT 1 cut(s) 389
BsuI GTATCC 1 cut(s) 782
BsuRI GGCC 3 cut(s) 734, 846, 1023
BsuTUI ATCGAT 1 cut(s) 389
BtgZI GCGATG 1 cut(s) 784
BtsCI GGATG 2 cut(s) 331, 817
BveI ACCTGC 2 cut(s) 431, 751
Cac8I GCNNGC 3 cut(s) 239, 561, 758
CciI TCATGA 1 cut(s) 919
Cfr13I GGNCC 3 cut(s) 41, 467, 774
ClaI ATCGAT 1 cut(s) 389
CseI GACGC 1 cut(s) 809
Csp6I GTAC 3 cut(s) 100, 477, 715
CviAII CATG 6 cut(s) 418, 480, 538, 796, 848, 920
CviQI GTAC 3 cut(s) 100, 477, 715
DdeI CTNAG 6 cut(s) 5, 333, 375, 543, 816, 1029
DpnI GATC 5 cut(s) 121, 388, 392, 588, 619
DpnII GATC 5 cut(s) 119, 386, 390, 586, 617
DriI GACNNNNNGTC 1 cut(s) 208
Eam1104I CTCTTC 1 cut(s) 833
Eam1105I GACNNNNNGTC 1 cut(s) 208
EarI CTCTTC 1 cut(s) 833
Ecl136II GAGCTC 1 cut(s) 635
Eco130I CCWWGG 1 cut(s) 403
Eco147I AGGCCT 1 cut(s) 734
Eco24I GRGCYC 1 cut(s) 637
Eco47I GGWCC 3 cut(s) 41, 467, 774
Eco53kI GAGCTC 1 cut(s) 635
EcoICRI GAGCTC 1 cut(s) 635
EcoRII CCWGG 4 cut(s) 86, 734, 861, 912
EcoT14I CCWWGG 1 cut(s) 403
EcoT38I GRGCYC 1 cut(s) 637
ErhI CCWWGG 1 cut(s) 403
FaeI CATG 6 cut(s) 421, 483, 541, 799, 851, 923
FatI CATG 6 cut(s) 417, 479, 537, 795, 847, 919
FauNDI CATATG 2 cut(s) 124, 217
FbaI TGATCA 1 cut(s) 586
Fnu4HI GCNGC 1 cut(s) 33
FokI GGATG 2 cut(s) 338, 824
FriOI GRGCYC 1 cut(s) 637
Fsp4HI GCNGC 1 cut(s) 33
GluI GCNGC 1 cut(s) 33
GsuI CTGGAG 2 cut(s) 183, 884
HaeIII GGCC 3 cut(s) 734, 846, 1023
HapII CCGG 2 cut(s) 167, 281
HgaI GACGC 1 cut(s) 809
Hin1II CATG 6 cut(s) 421, 483, 541, 799, 851, 923
HinfI GANTC 3 cut(s) 271, 329, 964
HpaII CCGG 2 cut(s) 167, 281
HphI GGTGA 3 cut(s) 82, 667, 988
Hpy166II GTNNAC 2 cut(s) 667, 893
Hpy188I TCNGA 4 cut(s) 336, 467, 969, 1030
Hpy188III TCNNGA 6 cut(s) 14, 44, 167, 200, 920, 961
Hpy8I GTNNAC 2 cut(s) 667, 893
HpyAV CCTTC 1 cut(s) 793
HpyCH4III ACNGT 4 cut(s) 298, 476, 664, 903
HpyCH4IV ACGT 2 cut(s) 60, 601
HpyCH4V TGCA 9 cut(s) 174, 426, 489, 559, 667, 756, 841, 884, 898
HpyF10VI GCNNNNNNNGC 2 cut(s) 459, 766
HpyF3I CTNAG 6 cut(s) 5, 333, 375, 543, 816, 1029
HpySE526I ACGT 2 cut(s) 60, 601
Hsp92II CATG 6 cut(s) 421, 483, 541, 799, 851, 923
Kpn2I TCCGGA 1 cut(s) 166
Ksp22I TGATCA 1 cut(s) 586
Kzo9I GATC 5 cut(s) 119, 386, 390, 586, 617
LmnI GCTCC 3 cut(s) 169, 202, 436
Lsp1109I GCAGC 1 cut(s) 44
LweI GCATC 2 cut(s) 184, 802
MaeII ACGT 2 cut(s) 60, 601
MaeIII GTNAC 4 cut(s) 70, 380, 976, 1003
MalI GATC 5 cut(s) 121, 388, 392, 588, 619
MboI GATC 5 cut(s) 119, 386, 390, 586, 617
MboII GAAGA 8 cut(s) 154, 157, 313, 328, 670, 820, 1054, 1055
MhlI GDGCHC 3 cut(s) 637, 669, 837
MluCI AATT 5 cut(s) 47, 256, 446, 747, 1069
MluI ACGCGT 1 cut(s) 492
MlyI GAGTC 1 cut(s) 973
MmeI TCCRAC 1 cut(s) 993
MnlI CCTC 8 cut(s) 256, 259, 473, 498, 724, 802, 861, 1009
MroI TCCGGA 1 cut(s) 166
MseI TTAA 7 cut(s) 50, 116, 186, 357, 498, 672, 1037
MslI CAYNNNNRTG 2 cut(s) 594, 879
MspI CCGG 2 cut(s) 167, 281
MspR9I CCNGG 4 cut(s) 88, 736, 863, 914
Mva1269I GAATGC 3 cut(s) 325, 561, 758
MvaI CCWGG 4 cut(s) 88, 736, 863, 914
MvnI CGCG 1 cut(s) 494
MwoI GCNNNNNNNGC 2 cut(s) 459, 766
NdeI CATATG 2 cut(s) 124, 217
NdeII GATC 5 cut(s) 119, 386, 390, 586, 617
NlaIII CATG 6 cut(s) 421, 483, 541, 799, 851, 923
NmeAIII GCCGAG 1 cut(s) 877
NmuCI GTSAC 2 cut(s) 70, 976
NspI RCATGY 1 cut(s) 483
NspV TTCGAA 1 cut(s) 1066
OliI CACNNNNGTG 1 cut(s) 879
PagI TCATGA 1 cut(s) 919
PceI AGGCCT 1 cut(s) 734
PctI GAATGC 3 cut(s) 325, 561, 758
PfeI GAWTC 2 cut(s) 271, 329
PkrI GCNGC 1 cut(s) 34
Ple19I CGATCG 1 cut(s) 393
PleI GAGTC 1 cut(s) 972
PpsI GAGTC 1 cut(s) 972
PshBI ATTAAT 1 cut(s) 50
Psp124BI GAGCTC 1 cut(s) 637
Psp1406I AACGTT 1 cut(s) 601
Psp6I CCWGG 4 cut(s) 86, 734, 861, 912
PspGI CCWGG 4 cut(s) 86, 734, 861, 912
PspPI GGNCC 3 cut(s) 41, 467, 774
PvuI CGATCG 1 cut(s) 393
RsaI GTAC 3 cut(s) 101, 478, 716
RsaNI GTAC 3 cut(s) 100, 477, 715
RseI CAYNNNNRTG 2 cut(s) 594, 879
SacI GAGCTC 1 cut(s) 637
SaqAI TTAA 7 cut(s) 50, 116, 186, 357, 498, 672, 1037
SatI GCNGC 1 cut(s) 33
Sau3AI GATC 5 cut(s) 119, 386, 390, 586, 617
Sau96I GGNCC 3 cut(s) 41, 467, 774
SchI GAGTC 1 cut(s) 973
ScrFI CCNGG 4 cut(s) 88, 736, 863, 914
SduI GDGCHC 3 cut(s) 637, 669, 837
SfaNI GCATC 2 cut(s) 184, 802
SfuI TTCGAA 1 cut(s) 1066
SinI GGWCC 3 cut(s) 41, 467, 774
SmiMI CAYNNNNRTG 2 cut(s) 594, 879
SmlI CTYRAG 1 cut(s) 638
SmoI CTYRAG 1 cut(s) 638
Sse9I AATT 5 cut(s) 47, 256, 446, 747, 1069
SseBI AGGCCT 1 cut(s) 734
SsiI CCGC 1 cut(s) 323
SstI GAGCTC 1 cut(s) 637
StuI AGGCCT 1 cut(s) 734
StyD4I CCNGG 4 cut(s) 86, 734, 861, 912
StyI CCWWGG 1 cut(s) 403
TaaI ACNGT 4 cut(s) 298, 476, 664, 903
TaiI ACGT 2 cut(s) 63, 604
TaqI TCGA 7 cut(s) 54, 129, 389, 393, 831, 993, 1066
TasI AATT 5 cut(s) 47, 256, 446, 747, 1069
TatI WGTACW 1 cut(s) 99
TfiI GAWTC 2 cut(s) 271, 329
Tru1I TTAA 7 cut(s) 50, 116, 186, 357, 498, 672, 1037
Tru9I TTAA 7 cut(s) 50, 116, 186, 357, 498, 672, 1037
TseFI GTSAC 2 cut(s) 70, 976
TseI GCWGC 1 cut(s) 32
Tsp45I GTSAC 2 cut(s) 70, 976
TspDTI ATGAA 3 cut(s) 612, 812, 928
VneI GTGCAC 1 cut(s) 665
VpaK11BI GGWCC 3 cut(s) 41, 467, 774
VspI ATTAAT 1 cut(s) 50
XceI RCATGY 1 cut(s) 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.