Rmu_co8438357.1_g000001

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8438357.1
OG0000864
Physical Location & Seq
Reverse (-)
2 .. 1523
1522 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8438357.1_g000001.1.cds

Sequence Viewer

Length: 1522 bp
atgaaaagggagagcagcagtggaagtagtgctagcgaggactccagttatcttaaagaagaagtaaatgtggactctgacgacgactcaaattatcttgaaggaggaaactcggatgccaatgtgttctctggggagggtcagtctccacggcagggttatactttactgagagaagaagaaattggccaacttcaggaggatgatattgcaggagtgtctactgtcctgtccatatcgaaatccgctgcatgcttcctactatgtcactttagttggagggtcaatagactgaaggacgaatggttttctgatgaagacaaagtccgggaaacagcaggcttgttgaggaagccggtgttcctgcatcgattaggtagtgtcaattgcggaatatgttttgatgactatgactattcatgcgatgaattagccggtggtactattttctcagctgcttgtggtcatcctttttgtaaagattgttggacaggttatattagcacatcgattaacgatggtccaggatgtttgatgctgagatgtcctgaaccatcttgtactgcagcagttgatctggatttgatccgaaatgtgttgaaaaaccaacatgatcacgagattgagaggtataagcgctaccttttgagatcttatgtcgaaaagcataagcagatgaagtggtgccctgctcctgactgtgagtatgctgtcaaagtgtttgatgaagatttgactggaaatggaagctgttatgatgtttcttgcagctgctcaaatagcttctgctggaattgtgttgaggaggttcatagtcctgttcagtgcgacactgtgggaaagtggatcttgaagaacaaggatgattcccagaatgcacaatggatagtggttaatactaagccctgtcccaagtgcaaaacaccgattgagaagaacaatgggtgtaatcacatgaggtgcagtccgccttgtgggcatcacttttgctggttatgcctcgggccacttggttgcaagtgcaatagtttcaatcgagctgcaaaccaaaatgcaaagaaggaggagagtcagagtgaaagggagaggaagaggctaaagatgagtttagagagatacaatcattattatgtgcgctgggcaaacaatcaagcttcaaagaaaaaggcagttgagtcattgcataaggcgcaaacagtgtacatcgtgaaacttgctagcaaaccaattccctttcttaccaagactaatgatttcgagttcattatagaggcatggaaacagataattgaatgtaggcaggtgatcaaatggacctatgcatatggttattacatacccgaggaaactaaatatgacaagaagcggcagctgtttgagtacttgcagggccaagcagagcacagcctggagaggcttcaccaatgtgccgagactgaaacgatagggtttgtcaaggattgggccccgttggaaaagttcccggacttccgtctaaagctcatacaattgacaaatgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IBR zf-RanBP

Protein Analysis

507

Amino Acids

58.05

Weight (kDa)

6.1

Isoelectric Point (pI)

51.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1291
Acc36I ACCTGC 1 cut(s) 1291
AccB1I GGYRCC 1 cut(s) 684
AccI GTMKAC 1 cut(s) 221
AciI CCGC 4 cut(s) 246, 390, 968, 1366
AclWI GGATC 2 cut(s) 580, 854
AcoI YGGCCR 1 cut(s) 187
AcuI CTGAAG 2 cut(s) 179, 314
AfaI GTAC 4 cut(s) 442, 562, 1202, 1382
AfeI AGCGCT 1 cut(s) 638
AfiI CCNNNNNNNGG 3 cut(s) 155, 196, 974
AgsI TTSAA 6 cut(s) 101, 601, 853, 1033, 1158, 1292
AjnI CCWGG 2 cut(s) 523, 1407
AjuI GAANNNNNNNTTGG 2 cut(s) 168, 200
AleI CACNNNNGTG 1 cut(s) 1425
AluBI AGCT 8 cut(s) 455, 750, 771, 783, 1040, 1154, 1372, 1501
AluI AGCT 8 cut(s) 455, 750, 771, 783, 1040, 1154, 1372, 1501
Alw21I GWGCWC 1 cut(s) 1404
Alw26I GTCTC 2 cut(s) 150, 1427
AlwI GGATC 2 cut(s) 580, 854
Ama87I CYCGRG 2 cut(s) 1001, 1340
Aor51HI AGCGCT 1 cut(s) 638
AoxI GGCC 4 cut(s) 187, 1004, 1390, 1464
ApaI GGGCCC 1 cut(s) 1468
ApeKI GCWGC 8 cut(s) 15, 248, 455, 566, 768, 771, 1040, 1369
AspLEI GCGC 3 cut(s) 639, 1137, 1192
AspS9I GGNCC 6 cut(s) 521, 1004, 1314, 1390, 1464, 1465
AsuC2I CCSGG 2 cut(s) 329, 1484
AsuHPI GGTGA 2 cut(s) 1315, 1412
AsuNHI GCTAGC 2 cut(s) 32, 1217
AvaI CYCGRG 2 cut(s) 1001, 1340
AvaII GGWCC 2 cut(s) 521, 1314
BaeGI GKGCMC 2 cut(s) 689, 1468
BalI TGGCCA 1 cut(s) 189
BanI GGYRCC 1 cut(s) 684
BanII GRGCYC 1 cut(s) 1468
BauI CACGAG 1 cut(s) 617
BbsI GAAGAC 1 cut(s) 324
Bbv12I GWGCWC 1 cut(s) 1404
BbvI GCAGC 8 cut(s) 27, 235, 442, 578, 758, 780, 1027, 1381
BccI CCATC 2 cut(s) 512, 562
BceAI ACGGC 1 cut(s) 167
BciT130I CCWGG 2 cut(s) 525, 1409
BclI TGATCA 2 cut(s) 613, 1305
BcnI CCSGG 2 cut(s) 329, 1484
BcoDI GTCTC 2 cut(s) 150, 1427
BfaI CTAG 2 cut(s) 33, 1218
BfmI CTRYAG 1 cut(s) 564
BfoI RGCGCY 1 cut(s) 640
BfuAI ACCTGC 1 cut(s) 1291
BglI GCCNNNNNGGC 1 cut(s) 976
BglII AGATCT 1 cut(s) 650
BisI GCNGC 9 cut(s) 16, 249, 456, 567, 769, 772, 1041, 1367, 1370
BlsI GCNGC 9 cut(s) 17, 250, 457, 568, 770, 773, 1042, 1368, 1371
BmcAI AGTACT 1 cut(s) 1382
Bme1390I CCNGG 4 cut(s) 329, 525, 1409, 1484
Bme18I GGWCC 2 cut(s) 521, 1314
BmeT110I CYCGRG 2 cut(s) 1001, 1340
BmgT120I GGNCC 6 cut(s) 521, 1004, 1314, 1390, 1464, 1465
BmiI GGNNCC 3 cut(s) 686, 1466, 1467
BmrFI CCNGG 4 cut(s) 329, 525, 1409, 1484
BmsI GCATC 4 cut(s) 106, 376, 525, 988
BmtI GCTAGC 2 cut(s) 36, 1221
BoxI GACNNNNGTC 1 cut(s) 1491
BpiI GAAGAC 1 cut(s) 324
BpmI CTGGAG 2 cut(s) 28, 1430
BpuMI CCSGG 2 cut(s) 329, 1484
Bsa29I ATCGAT 2 cut(s) 370, 509
BsaJI CCNNGG 3 cut(s) 149, 1000, 1341
BsaXI ACNNNNNCTCC 2 cut(s) 128, 158
Bsc4I CCNNNNNNNGG 3 cut(s) 155, 196, 974
Bse118I RCCGGY 2 cut(s) 355, 434
Bse1I ACTGG 2 cut(s) 45, 742
Bse3DI GCAATG 1 cut(s) 1178
BseBI CCWGG 2 cut(s) 525, 1409
BseCI ATCGAT 2 cut(s) 370, 509
BseDI CCNNGG 3 cut(s) 149, 1000, 1341
BseGI GGATG 5 cut(s) 121, 208, 466, 533, 868
BseLI CCNNNNNNNGG 3 cut(s) 155, 196, 974
BseMI GCAATG 1 cut(s) 1178
BseMII CTCAG 3 cut(s) 161, 465, 530
BseNI ACTGG 2 cut(s) 45, 742
BseRI GAGGAG 2 cut(s) 818, 1079
BseSI GKGCMC 2 cut(s) 689, 1468
BseXI GCAGC 8 cut(s) 27, 235, 442, 578, 758, 780, 1027, 1381
BseYI CCCAGC 1 cut(s) 1137
BsgI GTGCAG 1 cut(s) 982
BshFI GGCC 4 cut(s) 189, 1006, 1392, 1466
BshNI GGYRCC 1 cut(s) 684
BshVI ATCGAT 2 cut(s) 370, 509
BsiHKAI GWGCWC 1 cut(s) 1404
BsiHKCI CYCGRG 2 cut(s) 1001, 1340
BsiSI CCGG 4 cut(s) 328, 356, 435, 1484
BslFI GGGAC 1 cut(s) 894
BslI CCNNNNNNNGG 3 cut(s) 155, 196, 974
BsmAI GTCTC 2 cut(s) 150, 1427
BsmFI GGGAC 1 cut(s) 894
BsmI GAATGC 1 cut(s) 880
BsnI GGCC 4 cut(s) 189, 1006, 1392, 1466
BsoBI CYCGRG 2 cut(s) 1001, 1340
Bsp120I GGGCCC 1 cut(s) 1464
Bsp1286I GDGCHC 3 cut(s) 689, 1404, 1468
Bsp1407I TGTACA 1 cut(s) 1200
Bsp143I GATC 6 cut(s) 574, 585, 613, 650, 846, 1305
BspACI CCGC 4 cut(s) 246, 390, 968, 1366
BspANI GGCC 4 cut(s) 189, 1006, 1392, 1466
BspCNI CTCAG 3 cut(s) 162, 464, 531
BspDI ATCGAT 2 cut(s) 370, 509
BspLI GGNNCC 3 cut(s) 686, 1466, 1467
BspMAI CTGCAG 1 cut(s) 568
BspMI ACCTGC 1 cut(s) 1291
BspOI GCTAGC 2 cut(s) 36, 1221
BspPI GGATC 2 cut(s) 580, 854
BspT107I GGYRCC 1 cut(s) 684
BsrDI GCAATG 1 cut(s) 1178
BsrFI RCCGGY 2 cut(s) 355, 434
BsrGI TGTACA 1 cut(s) 1200
BsrI ACTGG 2 cut(s) 45, 742
BssAI RCCGGY 2 cut(s) 355, 434
BssECI CCNNGG 3 cut(s) 149, 1000, 1341
BssMI GATC 6 cut(s) 574, 585, 613, 650, 846, 1305
BssSI CACGAG 1 cut(s) 617
Bst2BI CACGAG 1 cut(s) 617
Bst2UI CCWGG 2 cut(s) 525, 1409
Bst4CI ACNGT 4 cut(s) 226, 701, 835, 1198
Bst6I CTCTTC 1 cut(s) 1085
BstAUI TGTACA 1 cut(s) 1200
BstC8I GCNNGC 4 cut(s) 34, 253, 340, 1219
BstDEI CTNAG 4 cut(s) 170, 451, 539, 900
BstDSI CCRYGG 1 cut(s) 149
BstF5I GGATG 5 cut(s) 121, 208, 466, 533, 868
BstH2I RGCGCY 1 cut(s) 640
BstHHI GCGC 3 cut(s) 639, 1137, 1192
BstKTI GATC 6 cut(s) 577, 588, 616, 653, 849, 1308
BstMAI GTCTC 2 cut(s) 150, 1427
BstMBI GATC 6 cut(s) 574, 585, 613, 650, 846, 1305
BstMWI GCNNNNNNNGC 4 cut(s) 780, 976, 996, 1189
BstNI CCWGG 2 cut(s) 525, 1409
BstNSI RCATGY 1 cut(s) 255
BstPAI GACNNNNGTC 1 cut(s) 1491
BstSCI CCNGG 4 cut(s) 327, 523, 1407, 1482
BstSFI CTRYAG 1 cut(s) 564
BstSLI GKGCMC 2 cut(s) 689, 1468
BstV1I GCAGC 8 cut(s) 27, 235, 442, 578, 758, 780, 1027, 1381
BstV2I GAAGAC 1 cut(s) 324
BstX2I RGATCY 2 cut(s) 650, 846
BstYI RGATCY 2 cut(s) 650, 846
Bsu15I ATCGAT 2 cut(s) 370, 509
BsuRI GGCC 4 cut(s) 189, 1006, 1392, 1466
BsuTUI ATCGAT 2 cut(s) 370, 509
BtgI CCRYGG 1 cut(s) 149
BtgZI GCGATG 1 cut(s) 438
BtsCI GGATG 5 cut(s) 121, 208, 466, 533, 868
BtsI GCAGTG 1 cut(s) 25
BtsIMutI CAGTG 4 cut(s) 25, 830, 831, 1203
BveI ACCTGC 1 cut(s) 1291
Cac8I GCNNGC 4 cut(s) 34, 253, 340, 1219
CfoI GCGC 3 cut(s) 639, 1137, 1192
Cfr10I RCCGGY 2 cut(s) 355, 434
Cfr13I GGNCC 6 cut(s) 521, 1004, 1314, 1390, 1464, 1465
ClaI ATCGAT 2 cut(s) 370, 509
Csp6I GTAC 4 cut(s) 441, 561, 1201, 1381
CviAII CATG 5 cut(s) 252, 420, 611, 955, 1275
CviQI GTAC 4 cut(s) 441, 561, 1201, 1381
DdeI CTNAG 4 cut(s) 170, 451, 539, 900
DpnI GATC 6 cut(s) 576, 587, 615, 652, 848, 1307
DpnII GATC 6 cut(s) 574, 585, 613, 650, 846, 1305
EaeI YGGCCR 1 cut(s) 187
Eam1104I CTCTTC 1 cut(s) 1085
EarI CTCTTC 1 cut(s) 1085
EciI GGCGGA 1 cut(s) 957
Eco24I GRGCYC 1 cut(s) 1468
Eco47I GGWCC 2 cut(s) 521, 1314
Eco47III AGCGCT 1 cut(s) 638
Eco57I CTGAAG 2 cut(s) 179, 314
Eco88I CYCGRG 2 cut(s) 1001, 1340
EcoO109I RGGNCCY 1 cut(s) 1465
EcoRII CCWGG 2 cut(s) 523, 1407
EcoT22I ATGCAT 1 cut(s) 1324
EcoT38I GRGCYC 1 cut(s) 1468
FaeI CATG 5 cut(s) 255, 423, 614, 958, 1278
FalI AAGNNNNNCTT 2 cut(s) 833, 865
FaqI GGGAC 1 cut(s) 894
FatI CATG 5 cut(s) 251, 419, 610, 954, 1274
FauNDI CATATG 1 cut(s) 1324
FbaI TGATCA 2 cut(s) 613, 1305
FblI GTMKAC 1 cut(s) 221
Fnu4HI GCNGC 9 cut(s) 16, 249, 456, 567, 769, 772, 1041, 1367, 1370
FokI GGATG 5 cut(s) 128, 215, 453, 540, 875
FriOI GRGCYC 1 cut(s) 1468
Fsp4HI GCNGC 9 cut(s) 16, 249, 456, 567, 769, 772, 1041, 1367, 1370
FspBI CTAG 2 cut(s) 33, 1218
GlaI GCGC 3 cut(s) 638, 1136, 1191
GluI GCNGC 9 cut(s) 16, 249, 456, 567, 769, 772, 1041, 1367, 1370
GsaI CCCAGC 1 cut(s) 1141
GsuI CTGGAG 2 cut(s) 28, 1430
HaeII RGCGCY 1 cut(s) 640
HaeIII GGCC 4 cut(s) 189, 1006, 1392, 1466
HapII CCGG 4 cut(s) 328, 356, 435, 1484
HhaI GCGC 3 cut(s) 639, 1137, 1192
Hin1II CATG 5 cut(s) 255, 423, 614, 958, 1278
Hin6I GCGC 3 cut(s) 637, 1135, 1190
HinP1I GCGC 3 cut(s) 637, 1135, 1190
HindIII AAGCTT 1 cut(s) 1152
HinfI GANTC 6 cut(s) 41, 74, 86, 866, 1069, 1175
HpaII CCGG 4 cut(s) 328, 356, 435, 1484
HphI GGTGA 2 cut(s) 1315, 1412
Hpy166II GTNNAC 3 cut(s) 73, 222, 1201
Hpy188I TCNGA 5 cut(s) 79, 115, 313, 590, 1074
Hpy188III TCNNGA 8 cut(s) 98, 197, 548, 578, 617, 695, 850, 1207
Hpy8I GTNNAC 3 cut(s) 73, 222, 1201
Hpy99I CGWCG 1 cut(s) 86
HpyAV CCTTC 3 cut(s) 95, 289, 1054
HpyCH4III ACNGT 4 cut(s) 226, 701, 835, 1198
HpyF10VI GCNNNNNNNGC 4 cut(s) 780, 976, 996, 1189
HpyF3I CTNAG 4 cut(s) 170, 451, 539, 900
Hsp92II CATG 5 cut(s) 255, 423, 614, 958, 1278
HspAI GCGC 3 cut(s) 637, 1135, 1190
Ksp22I TGATCA 2 cut(s) 613, 1305
Kzo9I GATC 6 cut(s) 574, 585, 613, 650, 846, 1305
LmnI GCTCC 1 cut(s) 697
Lsp1109I GCAGC 8 cut(s) 27, 235, 442, 578, 758, 780, 1027, 1381
LweI GCATC 4 cut(s) 106, 376, 525, 988
MaeI CTAG 2 cut(s) 33, 1218
MaeIII GTNAC 1 cut(s) 266
MalI GATC 6 cut(s) 576, 587, 615, 652, 848, 1307
MboI GATC 6 cut(s) 574, 585, 613, 650, 846, 1305
MboII GAAGA 8 cut(s) 71, 188, 191, 329, 740, 865, 946, 1102
MfeI CAATTG 2 cut(s) 385, 1508
MflI RGATCY 2 cut(s) 650, 846
MhlI GDGCHC 3 cut(s) 689, 1404, 1468
MlsI TGGCCA 1 cut(s) 189
MluCI AATT 8 cut(s) 91, 183, 385, 428, 793, 1227, 1287, 1508
MluNI TGGCCA 1 cut(s) 189
MlyI GAGTC 5 cut(s) 35, 68, 80, 1078, 1184
MmeI TCCRAC 3 cut(s) 257, 467, 1452
Mox20I TGGCCA 1 cut(s) 189
Mph1103I ATGCAT 1 cut(s) 1324
MscI TGGCCA 1 cut(s) 189
MseI TTAA 3 cut(s) 54, 513, 894
MslI CAYNNNNRTG 2 cut(s) 1128, 1425
Msp20I TGGCCA 1 cut(s) 189
MspA1I CMGCKG 4 cut(s) 248, 455, 771, 1372
MspI CCGG 4 cut(s) 328, 356, 435, 1484
MspR9I CCNGG 4 cut(s) 329, 525, 1409, 1484
MunI CAATTG 2 cut(s) 385, 1508
Mva1269I GAATGC 1 cut(s) 880
MvaI CCWGG 2 cut(s) 525, 1409
MwoI GCNNNNNNNGC 4 cut(s) 780, 976, 996, 1189
NciI CCSGG 2 cut(s) 329, 1484
NdeI CATATG 1 cut(s) 1324
NdeII GATC 6 cut(s) 574, 585, 613, 650, 846, 1305
NheI GCTAGC 2 cut(s) 32, 1217
NlaIII CATG 5 cut(s) 255, 423, 614, 958, 1278
NlaIV GGNNCC 3 cut(s) 686, 1466, 1467
NmeAIII GCCGAG 1 cut(s) 1456
NmuCI GTSAC 1 cut(s) 266
NsiI ATGCAT 1 cut(s) 1324
NspI RCATGY 1 cut(s) 255
OliI CACNNNNGTG 1 cut(s) 1425
PaeI GCATGC 1 cut(s) 255
PaqCI CACCTGC 1 cut(s) 1291
PctI GAATGC 1 cut(s) 880
PfeI GAWTC 1 cut(s) 866
PflFI GACNNNGTC 1 cut(s) 323
PfoI TCCNGGA 3 cut(s) 327, 523, 1482
PkrI GCNGC 9 cut(s) 17, 250, 457, 568, 770, 773, 1042, 1368, 1371
PleI GAGTC 5 cut(s) 35, 68, 80, 1077, 1183
PpsI GAGTC 5 cut(s) 35, 68, 80, 1077, 1183
PshAI GACNNNNGTC 1 cut(s) 1491
Psp6I CCWGG 2 cut(s) 523, 1407
PspFI CCCAGC 1 cut(s) 1137
PspGI CCWGG 2 cut(s) 523, 1407
PspN4I GGNNCC 3 cut(s) 686, 1466, 1467
PspOMI GGGCCC 1 cut(s) 1464
PspPI GGNCC 6 cut(s) 521, 1004, 1314, 1390, 1464, 1465
PstI CTGCAG 1 cut(s) 568
PsuI RGATCY 2 cut(s) 650, 846
PsyI GACNNNGTC 1 cut(s) 323
PvuII CAGCTG 3 cut(s) 455, 771, 1372
RsaI GTAC 4 cut(s) 442, 562, 1202, 1382
RsaNI GTAC 4 cut(s) 441, 561, 1201, 1381
RseI CAYNNNNRTG 2 cut(s) 1128, 1425
SaqAI TTAA 3 cut(s) 54, 513, 894
SatI GCNGC 9 cut(s) 16, 249, 456, 567, 769, 772, 1041, 1367, 1370
Sau3AI GATC 6 cut(s) 574, 585, 613, 650, 846, 1305
Sau96I GGNCC 6 cut(s) 521, 1004, 1314, 1390, 1464, 1465
ScaI AGTACT 1 cut(s) 1382
SchI GAGTC 5 cut(s) 35, 68, 80, 1078, 1184
ScrFI CCNGG 4 cut(s) 329, 525, 1409, 1484
SduI GDGCHC 3 cut(s) 689, 1404, 1468
SfaNI GCATC 4 cut(s) 106, 376, 525, 988
SfcI CTRYAG 1 cut(s) 564
SinI GGWCC 2 cut(s) 521, 1314
SmiMI CAYNNNNRTG 2 cut(s) 1128, 1425
SphI GCATGC 1 cut(s) 255
Sse9I AATT 8 cut(s) 91, 183, 385, 428, 793, 1227, 1287, 1508
SsiI CCGC 4 cut(s) 246, 390, 968, 1366
SspMI CTAG 2 cut(s) 33, 1218
StyD4I CCNGG 4 cut(s) 327, 523, 1407, 1482
TaaI ACNGT 4 cut(s) 226, 701, 835, 1198
TaqI TCGA 6 cut(s) 239, 370, 509, 660, 1036, 1257
TasI AATT 8 cut(s) 91, 183, 385, 428, 793, 1227, 1287, 1508
TatI WGTACW 3 cut(s) 560, 1200, 1380
TauI GCSGC 1 cut(s) 1369
TfiI GAWTC 1 cut(s) 866
Tru1I TTAA 3 cut(s) 54, 513, 894
Tru9I TTAA 3 cut(s) 54, 513, 894
TscAI CASTG 4 cut(s) 25, 830, 838, 1203
TseFI GTSAC 1 cut(s) 266
TseI GCWGC 8 cut(s) 15, 248, 455, 566, 768, 771, 1040, 1369
Tsp45I GTSAC 1 cut(s) 266
TspDTI ATGAA 8 cut(s) 17, 330, 408, 441, 692, 741, 800, 1252
TspGWI ACGGA 1 cut(s) 1481
TspRI CASTG 4 cut(s) 25, 830, 838, 1203
Tth111I GACNNNGTC 1 cut(s) 323
VpaK11BI GGWCC 2 cut(s) 521, 1314
XceI RCATGY 1 cut(s) 255
XmiI GTMKAC 1 cut(s) 221
XspI CTAG 2 cut(s) 33, 1218
ZrmI AGTACT 1 cut(s) 1382
Zsp2I ATGCAT 1 cut(s) 1324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.