Rorug06G0314900

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000864
Physical Location & Seq
Reverse (-)
46625965 .. 46626633
669 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0314900.1

Sequence Viewer

Length: 669 bp
ATGGGTGTTCTTAATGTTACAGCAGTTCTACTGCTATTTTTCATAAGCATGCAATTGTCTGAGGCTGGCAGGGTGTTGCATGACGAGGACGAAAACTTGACGAAGAAGGCTGTTCTTCCTCCCGGTGGACCTAATGGTCACACCAACGTGCCTGGTCCTGCGACTCCTACTAGTGGTCATGCTGCAACCATTAACCAAAGAAACTTTGCAGGCCACACTGCTACAGAAAACTTGACGAAGAACAAACTTTTCGTTTTAATGGAGTCGAAGCAGAAAGGTGGACGCCCTTCCCAGGGACCTAATGGTAACACCAACGTGCCTGGTCCTGCGACTCCTACTAGTGGTCATGCTGCAACCATTAACCAAAGAAACTTTGCAGGCCACACTGCTACAGAAAACTTGACGAAGAACAAACTTTTCGTTTTAATGGAGTCGAAGCAGAAAGGTGGACGCCCTTCCCAGGGACCTAATGGTAACACCAACGTGCCTGGTCCTGCGACTCCTACTAGTGGTCATGCTGCAACCATTAACCAAAGAAACTTTGCAGGCCACACCACTGTAGAAAACTTGACGAAGAACAAACTTTTTGTTTTAATGGAGTCGAAGCAGAGGGGTATTCCCCCTTCCGGTGGACCTAATCCGCCCACCAACAAGCCTGGTCATGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IBR zf-RanBP

Protein Analysis

222

Amino Acids

23.22

Weight (kDa)

10.24

Isoelectric Point (pI)

27.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 135
AciI CCGC 1 cut(s) 641
AcyI GRCGYC 2 cut(s) 283, 451
AhlI ACTAGT 3 cut(s) 170, 338, 506
AjnI CCWGG 6 cut(s) 151, 291, 319, 459, 487, 655
AleI CACNNNNGTG 3 cut(s) 146, 314, 482
AoxI GGCC 3 cut(s) 211, 379, 547
ApeKI GCWGC 3 cut(s) 182, 350, 518
AspS9I GGNCC 7 cut(s) 128, 155, 296, 323, 464, 491, 632
AsuC2I CCSGG 1 cut(s) 123
AvaII GGWCC 7 cut(s) 128, 155, 296, 323, 464, 491, 632
BarI GAAGNNNNNNTAC 2 cut(s) 607, 639
BbvI GCAGC 3 cut(s) 169, 337, 505
BciT130I CCWGG 6 cut(s) 153, 293, 321, 461, 489, 657
BcnI CCSGG 1 cut(s) 123
BcuI ACTAGT 3 cut(s) 170, 338, 506
BfaI CTAG 3 cut(s) 171, 339, 507
BfmI CTRYAG 3 cut(s) 222, 390, 558
BisI GCNGC 3 cut(s) 183, 351, 519
BlsI GCNGC 3 cut(s) 184, 352, 520
Bme1390I CCNGG 7 cut(s) 123, 153, 293, 321, 461, 489, 657
Bme18I GGWCC 7 cut(s) 128, 155, 296, 323, 464, 491, 632
BmgT120I GGNCC 7 cut(s) 128, 155, 296, 323, 464, 491, 632
BmiI GGNNCC 2 cut(s) 297, 465
BmrFI CCNGG 7 cut(s) 123, 153, 293, 321, 461, 489, 657
BpuMI CCSGG 1 cut(s) 123
BsaHI GRCGYC 2 cut(s) 283, 451
BsaJI CCNNGG 4 cut(s) 291, 292, 459, 460
BsaWI WCCGGW 1 cut(s) 626
BseBI CCWGG 6 cut(s) 153, 293, 321, 461, 489, 657
BseDI CCNNGG 4 cut(s) 291, 292, 459, 460
BseMII CTCAG 1 cut(s) 51
BseXI GCAGC 3 cut(s) 169, 337, 505
BshFI GGCC 3 cut(s) 213, 381, 549
BsiSI CCGG 2 cut(s) 123, 627
BslFI GGGAC 2 cut(s) 309, 477
BsmFI GGGAC 2 cut(s) 309, 477
BsnI GGCC 3 cut(s) 213, 381, 549
BspACI CCGC 1 cut(s) 641
BspANI GGCC 3 cut(s) 213, 381, 549
BspCNI CTCAG 1 cut(s) 52
BspLI GGNNCC 2 cut(s) 297, 465
BssECI CCNNGG 4 cut(s) 291, 292, 459, 460
BssNI GRCGYC 2 cut(s) 283, 451
Bst2UI CCWGG 6 cut(s) 153, 293, 321, 461, 489, 657
Bst4CI ACNGT 1 cut(s) 559
BstACI GRCGYC 2 cut(s) 283, 451
BstC8I GCNNGC 5 cut(s) 50, 67, 211, 379, 547
BstDEI CTNAG 1 cut(s) 60
BstNI CCWGG 6 cut(s) 153, 293, 321, 461, 489, 657
BstNSI RCATGY 1 cut(s) 52
BstSCI CCNGG 7 cut(s) 121, 151, 291, 319, 459, 487, 655
BstSFI CTRYAG 3 cut(s) 222, 390, 558
BstV1I GCAGC 3 cut(s) 169, 337, 505
BsuRI GGCC 3 cut(s) 213, 381, 549
BtsI GCAGTG 2 cut(s) 216, 384
BtsIMutI CAGTG 3 cut(s) 216, 384, 555
Cac8I GCNNGC 5 cut(s) 50, 67, 211, 379, 547
Cfr13I GGNCC 7 cut(s) 128, 155, 296, 323, 464, 491, 632
CseI GACGC 2 cut(s) 291, 459
CviAII CATG 6 cut(s) 49, 80, 179, 347, 515, 662
CviJI RGCY 6 cut(s) 65, 110, 213, 381, 549, 655
CviKI_1 RGCY 6 cut(s) 65, 110, 213, 381, 549, 655
DdeI CTNAG 1 cut(s) 60
DrdI GACNNNNNNGTC 1 cut(s) 135
DseDI GACNNNNNNGTC 1 cut(s) 135
EciI GGCGGA 1 cut(s) 630
Eco47I GGWCC 7 cut(s) 128, 155, 296, 323, 464, 491, 632
EcoO109I RGGNCCY 2 cut(s) 296, 464
EcoRII CCWGG 6 cut(s) 151, 291, 319, 459, 487, 655
EcoT22I ATGCAT 1 cut(s) 667
FaeI CATG 6 cut(s) 52, 83, 182, 350, 518, 665
FaiI YATR 8 cut(s) 44, 50, 81, 180, 348, 516, 663, 667
FaqI GGGAC 2 cut(s) 309, 477
FatI CATG 6 cut(s) 48, 79, 178, 346, 514, 661
Fnu4HI GCNGC 3 cut(s) 183, 351, 519
Fsp4HI GCNGC 3 cut(s) 183, 351, 519
FspBI CTAG 3 cut(s) 171, 339, 507
GluI GCNGC 3 cut(s) 183, 351, 519
HaeIII GGCC 3 cut(s) 213, 381, 549
HapII CCGG 2 cut(s) 123, 627
HgaI GACGC 2 cut(s) 291, 459
Hin1I GRCGYC 2 cut(s) 283, 451
Hin1II CATG 6 cut(s) 52, 83, 182, 350, 518, 665
HinfI GANTC 6 cut(s) 163, 263, 331, 431, 499, 599
HpaII CCGG 2 cut(s) 123, 627
Hpy166II GTNNAC 4 cut(s) 128, 281, 449, 632
Hpy188I TCNGA 1 cut(s) 61
Hpy8I GTNNAC 4 cut(s) 128, 281, 449, 632
HpyAV CCTTC 4 cut(s) 100, 297, 465, 633
HpyCH4III ACNGT 1 cut(s) 559
HpyCH4IV ACGT 3 cut(s) 147, 315, 483
HpyCH4V TGCA 9 cut(s) 52, 79, 185, 209, 353, 377, 521, 545, 665
HpyF3I CTNAG 1 cut(s) 60
HpySE526I ACGT 3 cut(s) 147, 315, 483
Hsp92I GRCGYC 2 cut(s) 283, 451
Hsp92II CATG 6 cut(s) 52, 83, 182, 350, 518, 665
Lsp1109I GCAGC 3 cut(s) 169, 337, 505
MaeI CTAG 3 cut(s) 171, 339, 507
MaeII ACGT 3 cut(s) 147, 315, 483
MaeIII GTNAC 4 cut(s) 16, 137, 305, 473
MboII GAAGA 5 cut(s) 107, 115, 250, 418, 586
MfeI CAATTG 1 cut(s) 53
MluCI AATT 1 cut(s) 53
MlyI GAGTC 6 cut(s) 157, 272, 325, 440, 493, 608
MnlI CCTC 4 cut(s) 55, 79, 129, 603
Mph1103I ATGCAT 1 cut(s) 667
MseI TTAA 7 cut(s) 12, 192, 257, 360, 425, 528, 593
MslI CAYNNNNRTG 4 cut(s) 47, 146, 314, 482
MspI CCGG 2 cut(s) 123, 627
MspR9I CCNGG 7 cut(s) 123, 153, 293, 321, 461, 489, 657
MunI CAATTG 1 cut(s) 53
MvaI CCWGG 6 cut(s) 153, 293, 321, 461, 489, 657
NciI CCSGG 1 cut(s) 123
NlaIII CATG 6 cut(s) 52, 83, 182, 350, 518, 665
NlaIV GGNNCC 2 cut(s) 297, 465
NmuCI GTSAC 1 cut(s) 137
NsiI ATGCAT 1 cut(s) 667
NspI RCATGY 1 cut(s) 52
OliI CACNNNNGTG 3 cut(s) 146, 314, 482
PaeI GCATGC 1 cut(s) 52
PasI CCCWGGG 2 cut(s) 292, 460
PkrI GCNGC 3 cut(s) 184, 352, 520
PleI GAGTC 6 cut(s) 157, 271, 325, 439, 493, 607
PpsI GAGTC 6 cut(s) 157, 271, 325, 439, 493, 607
PpuMI RGGWCCY 2 cut(s) 296, 464
Psp5II RGGWCCY 2 cut(s) 296, 464
Psp6I CCWGG 6 cut(s) 151, 291, 319, 459, 487, 655
PspGI CCWGG 6 cut(s) 151, 291, 319, 459, 487, 655
PspN4I GGNNCC 2 cut(s) 297, 465
PspPI GGNCC 7 cut(s) 128, 155, 296, 323, 464, 491, 632
PspPPI RGGWCCY 2 cut(s) 296, 464
RseI CAYNNNNRTG 4 cut(s) 47, 146, 314, 482
SaqAI TTAA 7 cut(s) 12, 192, 257, 360, 425, 528, 593
SatI GCNGC 3 cut(s) 183, 351, 519
Sau96I GGNCC 7 cut(s) 128, 155, 296, 323, 464, 491, 632
SchI GAGTC 6 cut(s) 157, 272, 325, 440, 493, 608
ScrFI CCNGG 7 cut(s) 123, 153, 293, 321, 461, 489, 657
SetI ASST 9 cut(s) 133, 150, 280, 301, 318, 448, 469, 486, 637
SfcI CTRYAG 3 cut(s) 222, 390, 558
SinI GGWCC 7 cut(s) 128, 155, 296, 323, 464, 491, 632
SmiMI CAYNNNNRTG 4 cut(s) 47, 146, 314, 482
SpeI ACTAGT 3 cut(s) 170, 338, 506
SphI GCATGC 1 cut(s) 52
Sse9I AATT 1 cut(s) 53
SsiI CCGC 1 cut(s) 641
SspMI CTAG 3 cut(s) 171, 339, 507
StyD4I CCNGG 7 cut(s) 121, 151, 291, 319, 459, 487, 655
TaaI ACNGT 1 cut(s) 559
TaiI ACGT 3 cut(s) 150, 318, 486
TaqI TCGA 3 cut(s) 266, 434, 602
TasI AATT 1 cut(s) 53
Tru1I TTAA 7 cut(s) 12, 192, 257, 360, 425, 528, 593
Tru9I TTAA 7 cut(s) 12, 192, 257, 360, 425, 528, 593
TscAI CASTG 3 cut(s) 223, 391, 562
TseFI GTSAC 1 cut(s) 137
TseI GCWGC 3 cut(s) 182, 350, 518
Tsp45I GTSAC 1 cut(s) 137
TspDTI ATGAA 1 cut(s) 31
TspRI CASTG 3 cut(s) 223, 391, 562
VpaK11BI GGWCC 7 cut(s) 128, 155, 296, 323, 464, 491, 632
XceI RCATGY 1 cut(s) 52
XcmI CCANNNNNNNNNTGG 2 cut(s) 299, 467
XspI CTAG 3 cut(s) 171, 339, 507
Zsp2I ATGCAT 1 cut(s) 667
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.