Rw6G035600

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000864
Physical Location & Seq
Reverse (-)
60203953 .. 60205444
1492 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G035600.1

Sequence Viewer

Length: 1056 bp
ATGGATGACACAGACTACTTGTACGACGACGACGGCTTTAGCGATTGTGACTACGACGACGACGATGATCAGTCGATTGAAGAGGAATCAAATGATCGGCAAAACTACACTGTTCTGAAAGAAGAAGAGATTTTCAACTGGAACGTGATCAATCTCAACGACGAGTGGTTCGCCGATGAAGATAGGATCCGGCAAAAGGTAGGTCTGTTGGAGCAGCCTGTGGTTCAATTCAATCCGAAAAACCAAAGAACTATTTGCGGTATATGTTTTGAGGTGTATGGCTATGGATCGATGTATTCGGCCGCCTGCGGTCATCGGTATTGTGGAGAGTGCTGGAGGGGTTACATCAGCACAGCGATCAAGGATGGCCCCGCGTGTTTGATGCTGAGGTGTCCCGAACCGCCGTGTAGGGCGGCGGTTGGTCCTGATTTGATCTCGGTCTTGGTGTCGGAACAGGAGTACGAGAGGTACAGGCACTACCTTTTGAGATCGTACGTCGAACAGCAGACCAAGATCAAGTGGTGTCCTGCTCCGGATTGTAAGTACGCTGTTAGGTTTGATAGTTATGAGACCAGAAGCTATGATGTTTCGTGTCTTTGTTCGCATAGCTATTGCTGGAATTGTACGGAGGAGAGTCACCGTCCGGTGGACTGTGAGACGGTGAGGAAGTGGGTTATGAAGAACAAGGACAGCTCGCAAAATGCGCAGTGGATATTGCTTGAACTCAATTTCATCATAGATGCATGGCAACAGATAATTGAATGTAGGCAGGTGCTGCGGTGGAGCTATGCGTATGGCTACTACATGCCTGAGGATGAGCGTCTTAAGGCTGATCTGTTTGAGCACTTGCAAGGTCATGCAGAGTACAGCCTCGAGAGGCTTCACAAATGCGCCGAGACTGAAATGCAACCATTTCTGGCTTCTGAGGAAGAATTAAAGGAGAGCTTCCACGCATTTCGCTTAAAACTCGAAAATATGACGAGGGCCACTAAAGATTACTTCAGGAAGCTGGTTACAGCCTTGGAGAATGGCCTTTCGGAGGTGGACGGCGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IBR zf-RanBP

Protein Analysis

351

Amino Acids

41.4

Weight (kDa)

4.66

Isoelectric Point (pI)

56.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_2 PF13923 86 - 117 7e-06 Zinc finger, C3HC4 type (RING finger)
IBR PF01485 155 - 218 3.1e-16 IBR domain, a half RING-finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 760
Acc16I TGCGCA 1 cut(s) 705
Acc36I ACCTGC 1 cut(s) 760
AccII CGCG 1 cut(s) 374
AccIII TCCGGA 1 cut(s) 532
AciI CCGC 8 cut(s) 258, 303, 309, 372, 401, 413, 416, 778
AclWI GGATC 3 cut(s) 181, 194, 295
AcoI YGGCCR 1 cut(s) 300
AcuI CTGAAG 1 cut(s) 985
AfaI GTAC 7 cut(s) 23, 461, 470, 494, 545, 625, 866
AfiI CCNNNNNNNGG 3 cut(s) 196, 646, 1039
AflII CTTAAG 1 cut(s) 824
AgsI TTSAA 6 cut(s) 80, 136, 227, 232, 722, 761
AluBI AGCT 6 cut(s) 579, 609, 693, 786, 945, 1009
AluI AGCT 6 cut(s) 579, 609, 693, 786, 945, 1009
Alw21I GWGCWC 1 cut(s) 846
Alw26I GTCTC 3 cut(s) 563, 650, 890
AlwI GGATC 3 cut(s) 181, 194, 295
AlwNI CAGNNNCTG 1 cut(s) 775
Ama87I CYCGRG 1 cut(s) 872
Aor13HI TCCGGA 1 cut(s) 532
AoxI GGCC 4 cut(s) 300, 367, 984, 1030
ApeKI GCWGC 2 cut(s) 214, 775
ArsI GACNNNNNNTTYG 4 cut(s) 152, 184, 187, 219
AspLEI GCGC 2 cut(s) 706, 893
AspS9I GGNCC 3 cut(s) 368, 422, 984
AsuHPI GGTGA 2 cut(s) 629, 673
AvaI CYCGRG 1 cut(s) 872
AvaII GGWCC 1 cut(s) 422
AxyI CCTNAGG 1 cut(s) 810
BamHI GGATCC 1 cut(s) 186
Bbv12I GWGCWC 1 cut(s) 846
BbvCI CCTCAGC 1 cut(s) 386
BbvI GCAGC 2 cut(s) 226, 762
BccI CCATC 1 cut(s) 359
BceAI ACGGC 2 cut(s) 49, 388
BclI TGATCA 2 cut(s) 67, 147
BcoDI GTCTC 3 cut(s) 563, 650, 890
BfrI CTTAAG 1 cut(s) 824
BfuAI ACCTGC 1 cut(s) 760
BisI GCNGC 4 cut(s) 215, 303, 414, 776
BlsI GCNGC 4 cut(s) 216, 304, 415, 777
Bme18I GGWCC 1 cut(s) 422
BmeT110I CYCGRG 1 cut(s) 872
BmgT120I GGNCC 3 cut(s) 368, 422, 984
BmiI GGNNCC 2 cut(s) 188, 370
BmsI GCATC 2 cut(s) 372, 730
BpmI CTGGAG 1 cut(s) 355
Bpu10I CCTNAGC 1 cut(s) 386
Bsa29I ATCGAT 1 cut(s) 290
BsaI GGTCTC 1 cut(s) 563
BsaJI CCNNGG 1 cut(s) 1020
BsaWI WCCGGW 2 cut(s) 532, 643
Bsc4I CCNNNNNNNGG 3 cut(s) 196, 646, 1039
Bse1I ACTGG 1 cut(s) 143
Bse21I CCTNAGG 1 cut(s) 810
BseAI TCCGGA 1 cut(s) 532
BseCI ATCGAT 1 cut(s) 290
BseDI CCNNGG 1 cut(s) 1020
BseGI GGATG 3 cut(s) 10, 370, 820
BseLI CCNNNNNNNGG 3 cut(s) 196, 646, 1039
BseMII CTCAG 3 cut(s) 377, 801, 915
BseNI ACTGG 1 cut(s) 143
BseRI GAGGAG 1 cut(s) 644
BseX3I CGGCCG 1 cut(s) 300
BseXI GCAGC 2 cut(s) 226, 762
Bsh1236I CGCG 1 cut(s) 374
Bsh1285I CGRYCG 1 cut(s) 303
BshFI GGCC 4 cut(s) 302, 369, 986, 1032
BshVI ATCGAT 1 cut(s) 290
BsiEI CGRYCG 1 cut(s) 303
BsiHKAI GWGCWC 1 cut(s) 846
BsiHKCI CYCGRG 1 cut(s) 872
BsiSI CCGG 3 cut(s) 190, 533, 644
BsiWI CGTACG 1 cut(s) 492
BslFI GGGAC 1 cut(s) 378
BslI CCNNNNNNNGG 3 cut(s) 196, 646, 1039
BsmAI GTCTC 3 cut(s) 563, 650, 890
BsmBI CGTCTC 1 cut(s) 650
BsmFI GGGAC 1 cut(s) 378
BsnI GGCC 4 cut(s) 302, 369, 986, 1032
Bso31I GGTCTC 1 cut(s) 563
BsoBI CYCGRG 1 cut(s) 872
Bsp1286I GDGCHC 1 cut(s) 846
Bsp13I TCCGGA 1 cut(s) 532
BspACI CCGC 8 cut(s) 258, 303, 309, 372, 401, 413, 416, 778
BspANI GGCC 4 cut(s) 302, 369, 986, 1032
BspCNI CTCAG 3 cut(s) 378, 802, 916
BspDI ATCGAT 1 cut(s) 290
BspEI TCCGGA 1 cut(s) 532
BspFNI CGCG 1 cut(s) 374
BspLI GGNNCC 2 cut(s) 188, 370
BspMI ACCTGC 1 cut(s) 760
BspPI GGATC 3 cut(s) 181, 194, 295
BspTI CTTAAG 1 cut(s) 824
BspTNI GGTCTC 1 cut(s) 563
BsrI ACTGG 1 cut(s) 143
BssECI CCNNGG 1 cut(s) 1020
BssT1I CCWWGG 1 cut(s) 1020
Bst4CI ACNGT 4 cut(s) 112, 641, 653, 661
Bst6I CTCTTC 2 cut(s) 75, 120
BstAFI CTTAAG 1 cut(s) 824
BstAPI GCANNNNNTGC 1 cut(s) 775
BstC8I GCNNGC 2 cut(s) 307, 695
BstDEI CTNAG 3 cut(s) 386, 810, 924
BstENI CCTNNNNNAGG 1 cut(s) 1037
BstF5I GGATG 3 cut(s) 10, 370, 820
BstFNI CGCG 1 cut(s) 374
BstHHI GCGC 2 cut(s) 706, 893
BstMAI GTCTC 3 cut(s) 563, 650, 890
BstMCI CGRYCG 1 cut(s) 303
BstMWI GCNNNNNNNGC 2 cut(s) 703, 775
BstNSI RCATGY 1 cut(s) 808
BstUI CGCG 1 cut(s) 374
BstV1I GCAGC 2 cut(s) 226, 762
BstX2I RGATCY 1 cut(s) 186
BstYI RGATCY 1 cut(s) 186
BstZI CGGCCG 1 cut(s) 300
Bsu15I ATCGAT 1 cut(s) 290
Bsu36I CCTNAGG 1 cut(s) 810
BsuRI GGCC 4 cut(s) 302, 369, 986, 1032
BsuTUI ATCGAT 1 cut(s) 290
BtsCI GGATG 3 cut(s) 10, 370, 820
BtsI GCAGTG 1 cut(s) 713
BtsIMutI CAGTG 2 cut(s) 108, 713
BveI ACCTGC 1 cut(s) 760
Cac8I GCNNGC 2 cut(s) 307, 695
CaiI CAGNNNCTG 1 cut(s) 775
CfoI GCGC 2 cut(s) 706, 893
Cfr13I GGNCC 3 cut(s) 368, 422, 984
ClaI ATCGAT 1 cut(s) 290
CseI GACGC 1 cut(s) 809
Csp6I GTAC 7 cut(s) 22, 460, 469, 493, 544, 624, 865
CviAII CATG 3 cut(s) 744, 805, 857
CviQI GTAC 7 cut(s) 22, 460, 469, 493, 544, 624, 865
DdeI CTNAG 3 cut(s) 386, 810, 924
EaeI YGGCCR 1 cut(s) 300
EagI CGGCCG 1 cut(s) 300
Eam1104I CTCTTC 2 cut(s) 75, 120
EarI CTCTTC 2 cut(s) 75, 120
EclXI CGGCCG 1 cut(s) 300
Eco130I CCWWGG 1 cut(s) 1020
Eco31I GGTCTC 1 cut(s) 563
Eco47I GGWCC 1 cut(s) 422
Eco52I CGGCCG 1 cut(s) 300
Eco57I CTGAAG 1 cut(s) 985
Eco81I CCTNAGG 1 cut(s) 810
Eco88I CYCGRG 1 cut(s) 872
EcoNI CCTNNNNNAGG 1 cut(s) 1037
EcoT14I CCWWGG 1 cut(s) 1020
EcoT22I ATGCAT 1 cut(s) 745
ErhI CCWWGG 1 cut(s) 1020
Esp3I CGTCTC 1 cut(s) 650
FaeI CATG 3 cut(s) 747, 808, 860
FalI AAGNNNNNCTT 2 cut(s) 929, 961
FaqI GGGAC 1 cut(s) 378
FatI CATG 3 cut(s) 743, 804, 856
FauI CCCGC 1 cut(s) 379
FbaI TGATCA 2 cut(s) 67, 147
Fnu4HI GCNGC 4 cut(s) 215, 303, 414, 776
FokI GGATG 3 cut(s) 17, 377, 827
Fsp4HI GCNGC 4 cut(s) 215, 303, 414, 776
FspI TGCGCA 1 cut(s) 705
GlaI GCGC 2 cut(s) 705, 892
GluI GCNGC 4 cut(s) 215, 303, 414, 776
GsuI CTGGAG 1 cut(s) 355
HaeIII GGCC 4 cut(s) 302, 369, 986, 1032
HapII CCGG 3 cut(s) 190, 533, 644
HgaI GACGC 1 cut(s) 809
HhaI GCGC 2 cut(s) 706, 893
Hin1II CATG 3 cut(s) 747, 808, 860
Hin6I GCGC 2 cut(s) 704, 891
HinP1I GCGC 2 cut(s) 704, 891
HinfI GANTC 2 cut(s) 86, 634
HpaII CCGG 3 cut(s) 190, 533, 644
HphI GGTGA 2 cut(s) 629, 673
Hpy166II GTNNAC 2 cut(s) 649, 1045
Hpy188I TCNGA 5 cut(s) 117, 237, 451, 925, 1039
Hpy188III TCNNGA 5 cut(s) 395, 425, 533, 874, 1003
Hpy8I GTNNAC 2 cut(s) 649, 1045
Hpy99I CGWCG 8 cut(s) 29, 32, 35, 59, 62, 65, 164, 500
HpyCH4III ACNGT 4 cut(s) 112, 641, 653, 661
HpyCH4IV ACGT 2 cut(s) 144, 495
HpyCH4V TGCA 4 cut(s) 743, 850, 860, 907
HpyF10VI GCNNNNNNNGC 2 cut(s) 703, 775
HpyF3I CTNAG 3 cut(s) 386, 810, 924
HpySE526I ACGT 2 cut(s) 144, 495
Hsp92II CATG 3 cut(s) 747, 808, 860
HspAI GCGC 2 cut(s) 704, 891
Kpn2I TCCGGA 1 cut(s) 532
Ksp22I TGATCA 2 cut(s) 67, 147
LmnI GCTCC 3 cut(s) 211, 535, 783
Lsp1109I GCAGC 2 cut(s) 226, 762
LweI GCATC 2 cut(s) 372, 730
MaeII ACGT 2 cut(s) 144, 495
MaeIII GTNAC 4 cut(s) 47, 341, 635, 1012
MboII GAAGA 6 cut(s) 92, 134, 137, 191, 691, 941
MflI RGATCY 1 cut(s) 186
MhlI GDGCHC 1 cut(s) 846
MluCI AATT 5 cut(s) 227, 619, 727, 756, 932
MlyI GAGTC 1 cut(s) 643
MmeI TCCRAC 2 cut(s) 189, 429
Mph1103I ATGCAT 1 cut(s) 745
MroI TCCGGA 1 cut(s) 532
MseI TTAA 3 cut(s) 825, 935, 962
MspCI CTTAAG 1 cut(s) 824
MspI CCGG 3 cut(s) 190, 533, 644
MvnI CGCG 1 cut(s) 374
MwoI GCNNNNNNNGC 2 cut(s) 703, 775
NlaIII CATG 3 cut(s) 747, 808, 860
NlaIV GGNNCC 2 cut(s) 188, 370
NmeAIII GCCGAG 1 cut(s) 919
NmuCI GTSAC 2 cut(s) 47, 635
NsbI TGCGCA 1 cut(s) 705
NsiI ATGCAT 1 cut(s) 745
NspI RCATGY 1 cut(s) 808
PaeR7I CTCGAG 1 cut(s) 872
PaqCI CACCTGC 1 cut(s) 760
PcsI WCGNNNNNNNCGW 2 cut(s) 39, 60
PfeI GAWTC 1 cut(s) 86
Pfl23II CGTACG 1 cut(s) 492
PkrI GCNGC 4 cut(s) 216, 304, 415, 777
PleI GAGTC 1 cut(s) 642
PpsI GAGTC 1 cut(s) 642
PspLI CGTACG 1 cut(s) 492
PspN4I GGNNCC 2 cut(s) 188, 370
PspPI GGNCC 3 cut(s) 368, 422, 984
PstNI CAGNNNCTG 1 cut(s) 775
PsuI RGATCY 1 cut(s) 186
RsaI GTAC 7 cut(s) 23, 461, 470, 494, 545, 625, 866
RsaNI GTAC 7 cut(s) 22, 460, 469, 493, 544, 624, 865
SaqAI TTAA 3 cut(s) 825, 935, 962
SatI GCNGC 4 cut(s) 215, 303, 414, 776
Sau96I GGNCC 3 cut(s) 368, 422, 984
SchI GAGTC 1 cut(s) 643
SduI GDGCHC 1 cut(s) 846
SfaNI GCATC 2 cut(s) 372, 730
Sfr274I CTCGAG 1 cut(s) 872
SinI GGWCC 1 cut(s) 422
SlaI CTCGAG 1 cut(s) 872
SmlI CTYRAG 2 cut(s) 824, 872
SmoI CTYRAG 2 cut(s) 824, 872
Sse9I AATT 5 cut(s) 227, 619, 727, 756, 932
SsiI CCGC 8 cut(s) 258, 303, 309, 372, 401, 413, 416, 778
StyI CCWWGG 1 cut(s) 1020
TaaI ACNGT 4 cut(s) 112, 641, 653, 661
TaiI ACGT 2 cut(s) 147, 498
TaqI TCGA 5 cut(s) 74, 290, 498, 873, 969
TaqII GACCGA 1 cut(s) 427
TasI AATT 5 cut(s) 227, 619, 727, 756, 932
TatI WGTACW 1 cut(s) 864
TauI GCSGC 2 cut(s) 305, 416
TfiI GAWTC 1 cut(s) 86
Tru1I TTAA 3 cut(s) 825, 935, 962
Tru9I TTAA 3 cut(s) 825, 935, 962
TscAI CASTG 2 cut(s) 115, 713
TseFI GTSAC 2 cut(s) 47, 635
TseI GCWGC 2 cut(s) 214, 775
Tsp45I GTSAC 2 cut(s) 47, 635
TspDTI ATGAA 3 cut(s) 192, 692, 721
TspGWI ACGGA 1 cut(s) 641
TspRI CASTG 2 cut(s) 115, 713
Vha464I CTTAAG 1 cut(s) 824
VpaK11BI GGWCC 1 cut(s) 422
XagI CCTNNNNNAGG 1 cut(s) 1037
XceI RCATGY 1 cut(s) 808
XhoI CTCGAG 1 cut(s) 872
Zsp2I ATGCAT 1 cut(s) 745
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.