Rmu_sc0001670.1_g000027

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001670.1
OG0000864
Physical Location & Seq
Forward (+)
132308 .. 133788
1481 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001670.1_g000027.1.cds

Sequence Viewer

Length: 1392 bp
atggcgcgctccgatcgaacaccaagctatgacgtagctactagtaactttcgttttcctccttttgcacgatcaaagtgttcttcgaattgggctgtcatccgttctgcatcacaaaatctaacaactacttgcgaaatatacttcgaggagtactactattcattagatgcatcatctaggagtaatagtattctcacagcctcttgtggacatccttattgtcgagattgctgggcaggttatataagcacgtcaatcggtgagggtggtcctgcctgtttgatgctcaggtgtcctcaaccgaagtgtagtgtagcagttggtcatgatttaatccaaaaggtgttgttgactacaaaccacggaactgcctgtgacaagtacaagcgctaccagttaagatcttatgtcgaaaatcacaggaagatcaaatgttattgctggaattgcaccgaggagaatcatagtccggtggactgcgagacggtggcgaagtggaatttgaagaacaaggatgattctcaaaatgcacattggatactggttcattccaagccttgtccaaggtgcaaaaaaccaattgagaagaaccaaggctgtaatcacatgaggtgcagatgtgggcagcaattctgctggtcatgcctcgggccttcgtatggaggctacaattgcaataaatacagatcatctgggacagggatgagtgctgtcgagagccaaaagaagaaggcgaagatgtatttagagagataccttcattattttgaacgctgggacaacaatggaaaatcaaagcaaaaggctcttgaggattttgataaggtgaagaatgtgcacatacatgaacttggaaagatacagggaaatacaaaagagcatgaatacaatttcatcatagaggcctggcaacagataataatatgcaggcaagtactgcaatggacctatgcttatggctactacatgagtgatgatgagcctcgcaagagtcagctattcgagatcttgcaaggccatgccgagttcagcctcgagaggcttcacaagtgtgccgagagcgaactgcagcagtttcttgtctgtggtgataacaatgaccacttgctgaaaatttacggggattttagaaagagactcataaacctgacaaaggtgactggaacttattttgagaagctggctagtgcactggaggagggccttcctgagacgtgcccctatgatgaggctagttggacctgcgaccagtgtacttatatcaatgtggggactgccatcacctgtgacgtgtgtaatatggaacgtaatggttggtgttgcgacctctgtacttatcggaatccagcctctgccatgagctatgagatgtgttcttatgctgagtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IBR zf-RanBP

Protein Analysis

463

Amino Acids

53.38

Weight (kDa)

7.95

Isoelectric Point (pI)

52.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 230, 1253
AccII CGCG 1 cut(s) 7
AcsI RAATTY 2 cut(s) 502, 1116
AfaI GTAC 5 cut(s) 155, 386, 939, 1258, 1336
AfeI AGCGCT 1 cut(s) 392
AfiI CCNNNNNNNGG 2 cut(s) 662, 1156
AflIII ACRYGT 1 cut(s) 1293
AgsI TTSAA 2 cut(s) 508, 773
AhlI ACTAGT 1 cut(s) 41
AjiI CACGTC 3 cut(s) 255, 1218, 1294
AjnI CCWGG 1 cut(s) 908
AleI CACNNNNGTG 1 cut(s) 1053
AluBI AGCT 5 cut(s) 27, 38, 1000, 1183, 1365
AluI AGCT 5 cut(s) 27, 38, 1000, 1183, 1365
Alw21I GWGCWC 2 cut(s) 843, 1195
Alw26I GTCTC 3 cut(s) 479, 1132, 1208
Alw44I GTGCAC 2 cut(s) 839, 1191
AlwNI CAGNNNCTG 1 cut(s) 1355
Ama87I CYCGRG 2 cut(s) 650, 1037
Aor51HI AGCGCT 1 cut(s) 392
AoxI GGCC 4 cut(s) 653, 906, 1018, 1204
ApaLI GTGCAC 2 cut(s) 839, 1191
ApeKI GCWGC 2 cut(s) 628, 1072
ApoI RAATTY 2 cut(s) 502, 1116
AspLEI GCGC 3 cut(s) 7, 9, 393
AspS9I GGNCC 5 cut(s) 272, 653, 948, 1204, 1242
AsuHPI GGTGA 5 cut(s) 275, 841, 1103, 1171, 1276
AsuII TTCGAA 1 cut(s) 86
AvaI CYCGRG 2 cut(s) 650, 1037
AvaII GGWCC 3 cut(s) 272, 948, 1242
BaeGI GKGCMC 3 cut(s) 843, 1195, 1223
Bbv12I GWGCWC 2 cut(s) 843, 1195
BbvI GCAGC 2 cut(s) 640, 1084
BccI CCATC 1 cut(s) 1289
BcgI CGANNNNNNTGC 2 cut(s) 241, 275
BciT130I CCWGG 1 cut(s) 910
BciVI GTATCC 1 cut(s) 534
BcoDI GTCTC 3 cut(s) 479, 1132, 1208
BcuI ACTAGT 1 cut(s) 41
BfaI CTAG 4 cut(s) 42, 180, 1188, 1236
BfmI CTRYAG 1 cut(s) 1070
BfoI RGCGCY 1 cut(s) 394
BfuAI ACCTGC 2 cut(s) 230, 1253
BfuI GTATCC 1 cut(s) 534
BglII AGATCT 2 cut(s) 404, 1008
BisI GCNGC 2 cut(s) 629, 1073
BlsI GCNGC 2 cut(s) 630, 1074
BmcAI AGTACT 2 cut(s) 155, 939
Bme1390I CCNGG 1 cut(s) 910
Bme18I GGWCC 3 cut(s) 272, 948, 1242
BmeT110I CYCGRG 2 cut(s) 650, 1037
BmgBI CACGTC 3 cut(s) 255, 1218, 1294
BmgT120I GGNCC 5 cut(s) 272, 653, 948, 1204, 1242
BmrFI CCNGG 1 cut(s) 910
BmsI GCATC 4 cut(s) 119, 160, 182, 276
BpmI CTGGAG 1 cut(s) 1217
Bpu10I CCTNAGC 1 cut(s) 290
Bpu14I TTCGAA 1 cut(s) 86
BpuEI CTTGAG 1 cut(s) 833
BsaJI CCNNGG 5 cut(s) 364, 456, 566, 595, 649
BsaWI WCCGGW 1 cut(s) 472
BsaXI ACNNNNNCTCC 2 cut(s) 175, 205
Bsc4I CCNNNNNNNGG 2 cut(s) 662, 1156
Bse1I ACTGG 5 cut(s) 397, 549, 1168, 1200, 1252
Bse3DI GCAATG 1 cut(s) 950
BseBI CCWGG 1 cut(s) 910
BseDI CCNNGG 5 cut(s) 364, 456, 566, 595, 649
BseGI GGATG 4 cut(s) 99, 214, 523, 711
BseLI CCNNNNNNNGG 2 cut(s) 662, 1156
BseMI GCAATG 1 cut(s) 950
BseMII CTCAG 3 cut(s) 304, 1203, 1377
BseNI ACTGG 5 cut(s) 397, 549, 1168, 1200, 1252
BsePI GCGCGC 1 cut(s) 5
BseRI GAGGAG 3 cut(s) 164, 473, 1214
BseSI GKGCMC 3 cut(s) 843, 1195, 1223
BseXI GCAGC 2 cut(s) 640, 1084
BseYI CCCAGC 2 cut(s) 234, 777
BsgI GTGCAG 1 cut(s) 637
Bsh1236I CGCG 1 cut(s) 7
Bsh1285I CGRYCG 1 cut(s) 16
BshFI GGCC 4 cut(s) 655, 908, 1020, 1206
BsiEI CGRYCG 1 cut(s) 16
BsiHKAI GWGCWC 2 cut(s) 843, 1195
BsiHKCI CYCGRG 2 cut(s) 650, 1037
BsiSI CCGG 1 cut(s) 473
BslFI GGGAC 3 cut(s) 712, 794, 1288
BslI CCNNNNNNNGG 2 cut(s) 662, 1156
BsmAI GTCTC 3 cut(s) 479, 1132, 1208
BsmBI CGTCTC 2 cut(s) 479, 1208
BsmFI GGGAC 3 cut(s) 712, 794, 1288
BsnI GGCC 4 cut(s) 655, 908, 1020, 1206
BsoBI CYCGRG 2 cut(s) 650, 1037
Bsp119I TTCGAA 1 cut(s) 86
Bsp1286I GDGCHC 3 cut(s) 843, 1195, 1223
Bsp143I GATC 6 cut(s) 13, 71, 404, 429, 689, 1008
BspANI GGCC 4 cut(s) 655, 908, 1020, 1206
BspCNI CTCAG 3 cut(s) 303, 1204, 1378
BspFNI CGCG 1 cut(s) 7
BspHI TCATGA 1 cut(s) 328
BspMAI CTGCAG 1 cut(s) 1074
BspMI ACCTGC 2 cut(s) 230, 1253
BspT104I TTCGAA 1 cut(s) 86
BsrDI GCAATG 1 cut(s) 950
BsrI ACTGG 5 cut(s) 397, 549, 1168, 1200, 1252
BssECI CCNNGG 5 cut(s) 364, 456, 566, 595, 649
BssHII GCGCGC 1 cut(s) 5
BssMI GATC 6 cut(s) 13, 71, 404, 429, 689, 1008
BssT1I CCWWGG 2 cut(s) 566, 595
Bst2UI CCWGG 1 cut(s) 910
Bst4CI ACNGT 1 cut(s) 490
BstAPI GCANNNNNTGC 1 cut(s) 940
BstBI TTCGAA 1 cut(s) 86
BstC8I GCNNGC 3 cut(s) 7, 932, 1185
BstDEI CTNAG 3 cut(s) 290, 1212, 1386
BstDSI CCRYGG 1 cut(s) 364
BstENI CCTNNNNNAGG 1 cut(s) 1154
BstF5I GGATG 4 cut(s) 99, 214, 523, 711
BstFNI CGCG 1 cut(s) 7
BstH2I RGCGCY 1 cut(s) 394
BstHHI GCGC 3 cut(s) 7, 9, 393
BstKTI GATC 6 cut(s) 16, 74, 407, 432, 692, 1011
BstMAI GTCTC 3 cut(s) 479, 1132, 1208
BstMBI GATC 6 cut(s) 13, 71, 404, 429, 689, 1008
BstMCI CGRYCG 1 cut(s) 16
BstMWI GCNNNNNNNGC 4 cut(s) 450, 645, 675, 940
BstNI CCWGG 1 cut(s) 910
BstSCI CCNGG 1 cut(s) 908
BstSFI CTRYAG 1 cut(s) 1070
BstSLI GKGCMC 3 cut(s) 843, 1195, 1223
BstUI CGCG 1 cut(s) 7
BstV1I GCAGC 2 cut(s) 640, 1084
BstX2I RGATCY 2 cut(s) 404, 1008
BstYI RGATCY 2 cut(s) 404, 1008
BsuI GTATCC 1 cut(s) 534
BsuRI GGCC 4 cut(s) 655, 908, 1020, 1206
BtgI CCRYGG 1 cut(s) 364
BtrI CACGTC 3 cut(s) 255, 1218, 1294
BtsCI GGATG 4 cut(s) 99, 214, 523, 711
BtsIMutI CAGTG 2 cut(s) 1193, 1259
BveI ACCTGC 2 cut(s) 230, 1253
Cac8I GCNNGC 3 cut(s) 7, 932, 1185
CaiI CAGNNNCTG 1 cut(s) 1355
CciI TCATGA 1 cut(s) 328
CfoI GCGC 3 cut(s) 7, 9, 393
Cfr13I GGNCC 5 cut(s) 272, 653, 948, 1204, 1242
Csp6I GTAC 5 cut(s) 154, 385, 938, 1257, 1335
CviAII CATG 8 cut(s) 329, 610, 645, 848, 884, 970, 1022, 1360
CviQI GTAC 5 cut(s) 154, 385, 938, 1257, 1335
DdeI CTNAG 3 cut(s) 290, 1212, 1386
DpnI GATC 6 cut(s) 15, 73, 406, 431, 691, 1010
DpnII GATC 6 cut(s) 13, 71, 404, 429, 689, 1008
Eco130I CCWWGG 2 cut(s) 566, 595
Eco147I AGGCCT 1 cut(s) 908
Eco47I GGWCC 3 cut(s) 272, 948, 1242
Eco47III AGCGCT 1 cut(s) 392
Eco88I CYCGRG 2 cut(s) 650, 1037
EcoNI CCTNNNNNAGG 1 cut(s) 1154
EcoO109I RGGNCCY 1 cut(s) 1204
EcoRII CCWGG 1 cut(s) 908
EcoT14I CCWWGG 2 cut(s) 566, 595
EcoT22I ATGCAT 1 cut(s) 175
ErhI CCWWGG 2 cut(s) 566, 595
Esp3I CGTCTC 2 cut(s) 479, 1208
FaeI CATG 8 cut(s) 332, 613, 648, 851, 887, 973, 1025, 1363
FaqI GGGAC 3 cut(s) 712, 794, 1288
FatI CATG 8 cut(s) 328, 609, 644, 847, 883, 969, 1021, 1359
Fnu4HI GCNGC 2 cut(s) 629, 1073
FokI GGATG 4 cut(s) 86, 201, 530, 718
Fsp4HI GCNGC 2 cut(s) 629, 1073
FspBI CTAG 4 cut(s) 42, 180, 1188, 1236
GlaI GCGC 3 cut(s) 6, 8, 392
GluI GCNGC 2 cut(s) 629, 1073
GsaI CCCAGC 2 cut(s) 238, 781
GsuI CTGGAG 1 cut(s) 1217
HaeII RGCGCY 1 cut(s) 394
HaeIII GGCC 4 cut(s) 655, 908, 1020, 1206
HapII CCGG 1 cut(s) 473
HhaI GCGC 3 cut(s) 7, 9, 393
Hin1II CATG 8 cut(s) 332, 613, 648, 851, 887, 973, 1025, 1363
Hin6I GCGC 3 cut(s) 5, 7, 391
HinP1I GCGC 3 cut(s) 5, 7, 391
HincII GTYRAC 1 cut(s) 354
HindII GTYRAC 1 cut(s) 354
HinfI GANTC 5 cut(s) 463, 521, 994, 1140, 1345
HpaII CCGG 1 cut(s) 473
HphI GGTGA 5 cut(s) 275, 841, 1103, 1171, 1276
Hpy166II GTNNAC 6 cut(s) 212, 354, 478, 841, 1193, 1257
Hpy188I TCNGA 2 cut(s) 13, 1344
Hpy188III TCNNGA 7 cut(s) 227, 329, 718, 812, 1006, 1039, 1211
Hpy8I GTNNAC 6 cut(s) 212, 354, 478, 841, 1193, 1257
HpyAV CCTTC 4 cut(s) 666, 727, 770, 1217
HpyCH4III ACNGT 1 cut(s) 490
HpyCH4IV ACGT 5 cut(s) 33, 254, 1217, 1293, 1309
HpyF10VI GCNNNNNNNGC 4 cut(s) 450, 645, 675, 940
HpyF3I CTNAG 3 cut(s) 290, 1212, 1386
HpySE526I ACGT 5 cut(s) 33, 254, 1217, 1293, 1309
Hsp92II CATG 8 cut(s) 332, 613, 648, 851, 887, 973, 1025, 1363
HspAI GCGC 3 cut(s) 5, 7, 391
Kzo9I GATC 6 cut(s) 13, 71, 404, 429, 689, 1008
LmnI GCTCC 1 cut(s) 14
Lsp1109I GCAGC 2 cut(s) 640, 1084
LweI GCATC 4 cut(s) 119, 160, 182, 276
MaeI CTAG 4 cut(s) 42, 180, 1188, 1236
MaeII ACGT 5 cut(s) 33, 254, 1217, 1293, 1309
MaeIII GTNAC 4 cut(s) 44, 377, 1159, 1289
MalI GATC 6 cut(s) 15, 73, 406, 431, 691, 1010
MboI GATC 6 cut(s) 13, 71, 404, 429, 689, 1008
MboII GAAGA 7 cut(s) 75, 439, 520, 601, 742, 751, 844
MfeI CAATTG 2 cut(s) 582, 673
MflI RGATCY 2 cut(s) 404, 1008
MhlI GDGCHC 3 cut(s) 843, 1195, 1223
MluCI AATT 8 cut(s) 88, 448, 502, 582, 632, 673, 892, 1116
MlyI GAGTC 2 cut(s) 1003, 1134
MmeI TCCRAC 1 cut(s) 1220
Mph1103I ATGCAT 1 cut(s) 175
MseI TTAA 2 cut(s) 335, 401
MslI CAYNNNNRTG 2 cut(s) 846, 1053
MspI CCGG 1 cut(s) 473
MspR9I CCNGG 1 cut(s) 910
MunI CAATTG 2 cut(s) 582, 673
MvaI CCWGG 1 cut(s) 910
MvnI CGCG 1 cut(s) 7
MwoI GCNNNNNNNGC 4 cut(s) 450, 645, 675, 940
NdeII GATC 6 cut(s) 13, 71, 404, 429, 689, 1008
NlaIII CATG 8 cut(s) 332, 613, 648, 851, 887, 973, 1025, 1363
NmeAIII GCCGAG 2 cut(s) 1051, 1084
NmuCI GTSAC 3 cut(s) 377, 1159, 1289
NsiI ATGCAT 1 cut(s) 175
NspV TTCGAA 1 cut(s) 86
OliI CACNNNNGTG 1 cut(s) 1053
PaeR7I CTCGAG 1 cut(s) 1037
PagI TCATGA 1 cut(s) 328
PauI GCGCGC 1 cut(s) 5
PceI AGGCCT 1 cut(s) 908
PfeI GAWTC 3 cut(s) 463, 521, 1345
PkrI GCNGC 2 cut(s) 630, 1074
Ple19I CGATCG 1 cut(s) 16
PleI GAGTC 2 cut(s) 1002, 1134
PpsI GAGTC 2 cut(s) 1002, 1134
Psp6I CCWGG 1 cut(s) 908
PspFI CCCAGC 2 cut(s) 234, 777
PspGI CCWGG 1 cut(s) 908
PspPI GGNCC 5 cut(s) 272, 653, 948, 1204, 1242
PstI CTGCAG 1 cut(s) 1074
PstNI CAGNNNCTG 1 cut(s) 1355
PsuI RGATCY 2 cut(s) 404, 1008
PteI GCGCGC 1 cut(s) 5
PvuI CGATCG 1 cut(s) 16
RsaI GTAC 5 cut(s) 155, 386, 939, 1258, 1336
RsaNI GTAC 5 cut(s) 154, 385, 938, 1257, 1335
RseI CAYNNNNRTG 2 cut(s) 846, 1053
SaqAI TTAA 2 cut(s) 335, 401
SatI GCNGC 2 cut(s) 629, 1073
Sau3AI GATC 6 cut(s) 13, 71, 404, 429, 689, 1008
Sau96I GGNCC 5 cut(s) 272, 653, 948, 1204, 1242
ScaI AGTACT 2 cut(s) 155, 939
SchI GAGTC 2 cut(s) 1003, 1134
ScrFI CCNGG 1 cut(s) 910
SduI GDGCHC 3 cut(s) 843, 1195, 1223
SfaNI GCATC 4 cut(s) 119, 160, 182, 276
SfcI CTRYAG 1 cut(s) 1070
Sfr274I CTCGAG 1 cut(s) 1037
SfuI TTCGAA 1 cut(s) 86
SinI GGWCC 3 cut(s) 272, 948, 1242
SlaI CTCGAG 1 cut(s) 1037
SmiMI CAYNNNNRTG 2 cut(s) 846, 1053
SmlI CTYRAG 2 cut(s) 812, 1037
SmoI CTYRAG 2 cut(s) 812, 1037
SpeI ACTAGT 1 cut(s) 41
Sse9I AATT 8 cut(s) 88, 448, 502, 582, 632, 673, 892, 1116
SseBI AGGCCT 1 cut(s) 908
SspMI CTAG 4 cut(s) 42, 180, 1188, 1236
StuI AGGCCT 1 cut(s) 908
StyD4I CCNGG 1 cut(s) 908
StyI CCWWGG 2 cut(s) 566, 595
TaaI ACNGT 1 cut(s) 490
TaiI ACGT 5 cut(s) 36, 257, 1220, 1296, 1312
TaqI TCGA 8 cut(s) 16, 86, 147, 226, 414, 717, 1005, 1038
TasI AATT 8 cut(s) 88, 448, 502, 582, 632, 673, 892, 1116
TatI WGTACW 5 cut(s) 153, 384, 937, 1256, 1334
TfiI GAWTC 3 cut(s) 463, 521, 1345
Tru1I TTAA 2 cut(s) 335, 401
Tru9I TTAA 2 cut(s) 335, 401
TscAI CASTG 2 cut(s) 1200, 1259
TseFI GTSAC 3 cut(s) 377, 1159, 1289
TseI GCWGC 2 cut(s) 628, 1072
Tsp45I GTSAC 3 cut(s) 377, 1159, 1289
TspDTI ATGAA 6 cut(s) 153, 539, 752, 864, 886, 900
TspGWI ACGGA 2 cut(s) 92, 381
TspRI CASTG 2 cut(s) 1200, 1259
VneI GTGCAC 2 cut(s) 839, 1191
VpaK11BI GGWCC 3 cut(s) 272, 948, 1242
XagI CCTNNNNNAGG 1 cut(s) 1154
XapI RAATTY 2 cut(s) 502, 1116
XhoI CTCGAG 1 cut(s) 1037
XspI CTAG 4 cut(s) 42, 180, 1188, 1236
ZrmI AGTACT 2 cut(s) 155, 939
Zsp2I ATGCAT 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.