Rroxscaffold_7G00167950

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000864
Physical Location & Seq
Forward (+)
8930776 .. 8931426
651 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00167950.1

Sequence Viewer

Length: 651 bp
ATGTATTTAGAGAGATGTCTTCATTATTTTGAACGCTGGGACAACAATGGAAAATCAAAGCAAAAGGCTCTTGAGGATTTTGATAAGGTGAAGAATGTGCACATACATGAACTTGGAAAGATACAGGGAAATACAAAAGAGCACGAATACAACTTCATCATAGAGGCCTGGCAACAGATAATTGAATGCAGGCAGGTACTGCGATGGACCTATGCTTATGGCTACTACATGAGTGATGATGAGCCTCGCAAGCGTCAGCTATTCGAGATCTTGCAAGGCCATGCCGAGTTCAGCCTCGAGAGGCTTCACGAGTGTGCCGAGAGCGAACTGCAGCAGTTTCTTGTCTGTGGTGATAACAATGACCACTTGCTGAAAATTTACGGGGATTTTAGAAAGAGACTCATAAACCTGACAAAGGTGACTGCAAGTTATTTTAAGAAGCTGGCTAGTGCACTGGAGGAGGGGCTTCCTGAGATGTGCCCCTATGACGAGGCTAGTTGGACCTGCGACCAGTGTACTTATATCAATGTGGGGACTGCCATCACCTGTGACGTGTGTAATATGGAACGTCATGGTTGGTGTTGCGACCTCTGTACTTATCGGAATCCAGCCTCTGCCATGAGCTGTGAGATGTGTTCTTATGCTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IBR zf-RanBP

Protein Analysis

216

Amino Acids

25.47

Weight (kDa)

5.32

Isoelectric Point (pI)

57.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ariadne PF19422 5 - 156 2.2e-06 Ariadne domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 184, 512
AcsI RAATTY 1 cut(s) 375
AfaI GTAC 3 cut(s) 198, 517, 595
AfiI CCNNNNNNNGG 1 cut(s) 415
AflIII ACRYGT 1 cut(s) 552
AgsI TTSAA 2 cut(s) 32, 185
AjiI CACGTC 1 cut(s) 553
AjnI CCWGG 1 cut(s) 167
AleI CACNNNNGTG 1 cut(s) 312
AluBI AGCT 3 cut(s) 259, 442, 624
AluI AGCT 3 cut(s) 259, 442, 624
Alw21I GWGCWC 3 cut(s) 102, 144, 454
Alw26I GTCTC 1 cut(s) 391
Alw44I GTGCAC 2 cut(s) 98, 450
AlwNI CAGNNNCTG 2 cut(s) 199, 614
Ama87I CYCGRG 1 cut(s) 296
AoxI GGCC 2 cut(s) 165, 277
ApaLI GTGCAC 2 cut(s) 98, 450
ApeKI GCWGC 1 cut(s) 331
ApoI RAATTY 1 cut(s) 375
AspS9I GGNCC 2 cut(s) 207, 501
AsuHPI GGTGA 4 cut(s) 100, 362, 430, 535
AvaI CYCGRG 1 cut(s) 296
AvaII GGWCC 2 cut(s) 207, 501
BaeGI GKGCMC 3 cut(s) 102, 454, 482
BauI CACGAG 1 cut(s) 308
BbsI GAAGAC 1 cut(s) 11
Bbv12I GWGCWC 3 cut(s) 102, 144, 454
BbvI GCAGC 1 cut(s) 343
BccI CCATC 2 cut(s) 198, 548
BciT130I CCWGG 1 cut(s) 169
BcoDI GTCTC 1 cut(s) 391
BfaI CTAG 2 cut(s) 447, 495
BfmI CTRYAG 1 cut(s) 329
BfuAI ACCTGC 2 cut(s) 184, 512
BglII AGATCT 1 cut(s) 267
BisI GCNGC 1 cut(s) 332
BlsI GCNGC 1 cut(s) 333
Bme1390I CCNGG 1 cut(s) 169
Bme18I GGWCC 2 cut(s) 207, 501
BmeT110I CYCGRG 1 cut(s) 296
BmgBI CACGTC 1 cut(s) 553
BmgT120I GGNCC 2 cut(s) 207, 501
BmrFI CCNGG 1 cut(s) 169
BpiI GAAGAC 1 cut(s) 11
BpmI CTGGAG 1 cut(s) 476
BpuEI CTTGAG 1 cut(s) 92
Bsc4I CCNNNNNNNGG 1 cut(s) 415
Bse1I ACTGG 2 cut(s) 459, 511
BseBI CCWGG 1 cut(s) 169
BseLI CCNNNNNNNGG 1 cut(s) 415
BseMII CTCAG 2 cut(s) 462, 636
BseNI ACTGG 2 cut(s) 459, 511
BseRI GAGGAG 1 cut(s) 473
BseSI GKGCMC 3 cut(s) 102, 454, 482
BseXI GCAGC 1 cut(s) 343
BseYI CCCAGC 1 cut(s) 36
BshFI GGCC 2 cut(s) 167, 279
BsiHKAI GWGCWC 3 cut(s) 102, 144, 454
BsiHKCI CYCGRG 1 cut(s) 296
BslFI GGGAC 2 cut(s) 53, 547
BslI CCNNNNNNNGG 1 cut(s) 415
BsmAI GTCTC 1 cut(s) 391
BsmFI GGGAC 2 cut(s) 53, 547
BsmI GAATGC 1 cut(s) 191
BsnI GGCC 2 cut(s) 167, 279
BsoBI CYCGRG 1 cut(s) 296
Bsp1286I GDGCHC 4 cut(s) 102, 144, 454, 482
Bsp143I GATC 1 cut(s) 267
BspANI GGCC 2 cut(s) 167, 279
BspCNI CTCAG 2 cut(s) 463, 637
BspMAI CTGCAG 1 cut(s) 333
BspMI ACCTGC 2 cut(s) 184, 512
BsrI ACTGG 2 cut(s) 459, 511
BssMI GATC 1 cut(s) 267
BssSI CACGAG 1 cut(s) 308
Bst2BI CACGAG 1 cut(s) 308
Bst2UI CCWGG 1 cut(s) 169
BstAPI GCANNNNNTGC 1 cut(s) 199
BstC8I GCNNGC 3 cut(s) 191, 251, 444
BstDEI CTNAG 2 cut(s) 471, 645
BstENI CCTNNNNNAGG 1 cut(s) 413
BstKTI GATC 1 cut(s) 270
BstMAI GTCTC 1 cut(s) 391
BstMBI GATC 1 cut(s) 267
BstMWI GCNNNNNNNGC 2 cut(s) 199, 250
BstNI CCWGG 1 cut(s) 169
BstSCI CCNGG 1 cut(s) 167
BstSFI CTRYAG 1 cut(s) 329
BstSLI GKGCMC 3 cut(s) 102, 454, 482
BstV1I GCAGC 1 cut(s) 343
BstV2I GAAGAC 1 cut(s) 11
BstX2I RGATCY 1 cut(s) 267
BstYI RGATCY 1 cut(s) 267
BsuRI GGCC 2 cut(s) 167, 279
BtgZI GCGATG 1 cut(s) 217
BtrI CACGTC 1 cut(s) 553
BtsIMutI CAGTG 2 cut(s) 452, 518
BveI ACCTGC 2 cut(s) 184, 512
Cac8I GCNNGC 3 cut(s) 191, 251, 444
CaiI CAGNNNCTG 2 cut(s) 199, 614
Cfr13I GGNCC 2 cut(s) 207, 501
CseI GACGC 1 cut(s) 242
Csp6I GTAC 3 cut(s) 197, 516, 594
CviAII CATG 5 cut(s) 107, 229, 281, 572, 619
CviQI GTAC 3 cut(s) 197, 516, 594
DdeI CTNAG 2 cut(s) 471, 645
DpnI GATC 1 cut(s) 269
DpnII GATC 1 cut(s) 267
Eco147I AGGCCT 1 cut(s) 167
Eco47I GGWCC 2 cut(s) 207, 501
Eco88I CYCGRG 1 cut(s) 296
EcoNI CCTNNNNNAGG 1 cut(s) 413
EcoRII CCWGG 1 cut(s) 167
FaeI CATG 5 cut(s) 110, 232, 284, 575, 622
FaqI GGGAC 2 cut(s) 53, 547
FatI CATG 5 cut(s) 106, 228, 280, 571, 618
Fnu4HI GCNGC 1 cut(s) 332
Fsp4HI GCNGC 1 cut(s) 332
FspBI CTAG 2 cut(s) 447, 495
GluI GCNGC 1 cut(s) 332
GsaI CCCAGC 1 cut(s) 40
GsuI CTGGAG 1 cut(s) 476
HaeIII GGCC 2 cut(s) 167, 279
HgaI GACGC 1 cut(s) 242
Hin1II CATG 5 cut(s) 110, 232, 284, 575, 622
HinfI GANTC 2 cut(s) 399, 604
HphI GGTGA 4 cut(s) 100, 362, 430, 535
Hpy166II GTNNAC 3 cut(s) 100, 452, 516
Hpy188I TCNGA 1 cut(s) 603
Hpy188III TCNNGA 5 cut(s) 71, 265, 298, 308, 470
Hpy8I GTNNAC 3 cut(s) 100, 452, 516
HpyCH4IV ACGT 2 cut(s) 552, 568
HpyCH4V TGCA 6 cut(s) 100, 189, 274, 331, 425, 452
HpyF10VI GCNNNNNNNGC 2 cut(s) 199, 250
HpyF3I CTNAG 2 cut(s) 471, 645
HpySE526I ACGT 2 cut(s) 552, 568
Hsp92II CATG 5 cut(s) 110, 232, 284, 575, 622
Kzo9I GATC 1 cut(s) 267
Lsp1109I GCAGC 1 cut(s) 343
MaeI CTAG 2 cut(s) 447, 495
MaeII ACGT 2 cut(s) 552, 568
MaeIII GTNAC 2 cut(s) 418, 548
MalI GATC 1 cut(s) 269
MboI GATC 1 cut(s) 267
MboII GAAGA 2 cut(s) 11, 103
MflI RGATCY 1 cut(s) 267
MhlI GDGCHC 4 cut(s) 102, 144, 454, 482
MluCI AATT 2 cut(s) 180, 375
MlyI GAGTC 1 cut(s) 393
MmeI TCCRAC 1 cut(s) 479
MseI TTAA 1 cut(s) 435
MslI CAYNNNNRTG 2 cut(s) 105, 312
MspR9I CCNGG 1 cut(s) 169
Mva1269I GAATGC 1 cut(s) 191
MvaI CCWGG 1 cut(s) 169
MwoI GCNNNNNNNGC 2 cut(s) 199, 250
NdeII GATC 1 cut(s) 267
NlaIII CATG 5 cut(s) 110, 232, 284, 575, 622
NmeAIII GCCGAG 2 cut(s) 310, 343
NmuCI GTSAC 2 cut(s) 418, 548
OliI CACNNNNGTG 1 cut(s) 312
PaeR7I CTCGAG 1 cut(s) 296
PceI AGGCCT 1 cut(s) 167
PcsI WCGNNNNNNNCGW 1 cut(s) 315
PctI GAATGC 1 cut(s) 191
PfeI GAWTC 1 cut(s) 604
PkrI GCNGC 1 cut(s) 333
PleI GAGTC 1 cut(s) 393
PpsI GAGTC 1 cut(s) 393
Psp6I CCWGG 1 cut(s) 167
PspFI CCCAGC 1 cut(s) 36
PspGI CCWGG 1 cut(s) 167
PspPI GGNCC 2 cut(s) 207, 501
PstI CTGCAG 1 cut(s) 333
PstNI CAGNNNCTG 2 cut(s) 199, 614
PsuI RGATCY 1 cut(s) 267
RsaI GTAC 3 cut(s) 198, 517, 595
RsaNI GTAC 3 cut(s) 197, 516, 594
RseI CAYNNNNRTG 2 cut(s) 105, 312
SaqAI TTAA 1 cut(s) 435
SatI GCNGC 1 cut(s) 332
Sau3AI GATC 1 cut(s) 267
Sau96I GGNCC 2 cut(s) 207, 501
SchI GAGTC 1 cut(s) 393
ScrFI CCNGG 1 cut(s) 169
SduI GDGCHC 4 cut(s) 102, 144, 454, 482
SfcI CTRYAG 1 cut(s) 329
Sfr274I CTCGAG 1 cut(s) 296
SinI GGWCC 2 cut(s) 207, 501
SlaI CTCGAG 1 cut(s) 296
SmiMI CAYNNNNRTG 2 cut(s) 105, 312
SmlI CTYRAG 2 cut(s) 71, 296
SmoI CTYRAG 2 cut(s) 71, 296
Sse9I AATT 2 cut(s) 180, 375
SseBI AGGCCT 1 cut(s) 167
SspMI CTAG 2 cut(s) 447, 495
StuI AGGCCT 1 cut(s) 167
StyD4I CCNGG 1 cut(s) 167
TaiI ACGT 2 cut(s) 555, 571
TaqI TCGA 2 cut(s) 264, 297
TasI AATT 2 cut(s) 180, 375
TatI WGTACW 2 cut(s) 515, 593
TfiI GAWTC 1 cut(s) 604
Tru1I TTAA 1 cut(s) 435
Tru9I TTAA 1 cut(s) 435
TscAI CASTG 2 cut(s) 459, 518
TseFI GTSAC 2 cut(s) 418, 548
TseI GCWGC 1 cut(s) 331
Tsp45I GTSAC 2 cut(s) 418, 548
TspDTI ATGAA 3 cut(s) 11, 123, 145
TspRI CASTG 2 cut(s) 459, 518
VneI GTGCAC 2 cut(s) 98, 450
VpaK11BI GGWCC 2 cut(s) 207, 501
XagI CCTNNNNNAGG 1 cut(s) 413
XapI RAATTY 1 cut(s) 375
XhoI CTCGAG 1 cut(s) 296
XspI CTAG 2 cut(s) 447, 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.