Rroxscaffold_7G00167970

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000864
Physical Location & Seq
Forward (+)
8932622 .. 8934136
1515 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00167970.1

Sequence Viewer

Length: 1515 bp
ATGGATGACACCGACTACATGTACGATGACACCGATGACAACTACGACGACGACGACGGCTTTAGCGATTGTGACGACGACGATGATGATCAGTCGATTGAAGAGGAATCAAATGATCGGCAAAACTACACTGTTCTGAAAGAAGAAGAGATTGCCAAGCTTCAAGAGGATGCTATCACTGAGGTGTCTGCTGTACTATCAATATCGCAATCCGCTGCCTGCTTCGTCCTCACTCAGTTCAACTGGAACGTGATCAATCTCAACGACGAGTGGTTCGCCGATGAAGACAGGATCCGGCAAAAGGTAGGTCTGTTGGAGCAGCCTGTGGTTCAATTCAATCCGAAAAACCAAAGAACTATTTGCGGTATATGTTTTGAGGTGTATGGCTATGGATCGATGTATTCGGCCGCCTGCGGTCATCGTTATTGTGGAGAGTGCTGGAGGGGTTACATCAGCACAGCGATCAAGGATGGCCCCGCGTGTTTGATGCTGAGGTGTCCCGAACCGCCGTGTAGGGCGGCGGTTGGTCCTGATTTGATCTCGGTCTTGGTGTCGAAACAGGAGTACGAGCGGTACAGGCACTACCTTTTGAGATCGTACGTCGAACAGCAGACCAAGATCAAGTGGTGTCCTGCTCCGGATTGTAAGTACGCTGTTAGGTTTGATAGTTATGAGACCAGAAGGTATGATGTTTCGTGTCTTTGTTCGCATACCTATTGCTGGAATTGTACGGAGGAGAGTCACCGTCCGGTGGACTGTGAGACGGTGAGGAAGTGGATTATGAAGAACAAGGACAGCTCGCAAAATGCGCAGTGGATATTGGTTAACACCAAGCCCTGTCCAAAGTGCAAGAGACCGATTCAGAAGAACCAAGGGTGTCACCATATGACTTGCTCAGCTCCTTGTAGGTATGAGTTTTGCTGGTATTGTCTTGCGAAATTCGAAAGTTATTCTACTAGATGTTGTAACGCGTATAGGGCGAATACTGATGAGCTGAAGGAGTTAGAGCTGGAGCCGGATCAGAAGAAGCTGAAGGAGTATTTAGATAGGTATCTTCATTATTATGAGCGATGGGCAAACAATCAGTCTTCGAAGGAAAAGGCTGTCGAGTCTTTAGAGGAGGTGAGAACCAAGCACATTATGGAGCTTATTAGGAAACAACAGTATCTGTCTGAGCTTGAACTCAATTTCATCATAGATGCATGGCAACAGATAATTGAATGTAGGCAGGTGCTGCGGTGGAGCTATGCGTATGGCTACTACATGCCTGAGGATGAGCGTCTTAAGGCTGATCTGTTTGAGCACTTGCAAGGTCATGCAGAGTACAGCCTCGAGAGGCTTCACAAATGCGCCGAGACTGAATTGCAACCATTTCTGGCTTCTGAGGAAGAATTAAAGGAGGGCTTCCATGCATTTCGCTTAAAACTCGAAAATATGACGAGGGCCACTAAAGATTATTTCAGGAAGCTGGTTACAGCCTTGGAGAATGGCCTTTCGGAGGTGGACGGCGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IBR zf-RanBP

Protein Analysis

504

Amino Acids

59.41

Weight (kDa)

4.97

Isoelectric Point (pI)

54.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UBA_ARI1 PF21235 54 - 92 1.5e-07 E3 ubiquitin-protein ligase ARIH1, UBA-like domain
IBR PF01485 190 - 253 1.5e-15 IBR domain, a half RING-finger domain
IBR_2 PF22605 273 - 315 6.3e-10 IBR domain
IBR_1 PF22191 276 - 315 2.1e-11 IBR domain
Ariadne PF19422 350 - 498 5.4e-07 Ariadne domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1219
Acc16I TGCGCA 1 cut(s) 810
Acc36I ACCTGC 1 cut(s) 1219
AccBSI CCGCTC 1 cut(s) 571
AccII CGCG 2 cut(s) 479, 971
AccIII TCCGGA 1 cut(s) 637
AclWI GGATC 4 cut(s) 286, 299, 400, 1026
AcoI YGGCCR 1 cut(s) 405
AcsI RAATTY 1 cut(s) 938
AcuI CTGAAG 2 cut(s) 1016, 1052
AfaI GTAC 8 cut(s) 23, 195, 566, 575, 599, 650, 730, 1325
AfiI CCNNNNNNNGG 4 cut(s) 301, 720, 751, 1498
AflII CTTAAG 1 cut(s) 1283
AflIII ACRYGT 2 cut(s) 18, 969
AgsI TTSAA 7 cut(s) 101, 164, 241, 332, 337, 1181, 1220
AleI CACNNNNGTG 1 cut(s) 182
Alw21I GWGCWC 1 cut(s) 1305
Alw26I GTCTC 4 cut(s) 668, 755, 847, 1349
AlwI GGATC 4 cut(s) 286, 299, 400, 1026
AlwNI CAGNNNCTG 2 cut(s) 1168, 1234
Ama87I CYCGRG 1 cut(s) 1331
Aor13HI TCCGGA 1 cut(s) 637
AoxI GGCC 4 cut(s) 405, 472, 1443, 1489
ApeKI GCWGC 3 cut(s) 215, 319, 1234
ApoI RAATTY 1 cut(s) 938
ArsI GACNNNNNNTTYG 6 cut(s) 257, 289, 292, 324, 1070, 1102
AspLEI GCGC 2 cut(s) 811, 1352
AspS9I GGNCC 3 cut(s) 473, 527, 1443
AsuHPI GGTGA 4 cut(s) 734, 778, 872, 1135
AsuII TTCGAA 2 cut(s) 942, 1091
AvaI CYCGRG 1 cut(s) 1331
AvaII GGWCC 1 cut(s) 527
AxyI CCTNAGG 1 cut(s) 1269
BaeI ACNNNNGTAYC 2 cut(s) 1148, 1181
BamHI GGATCC 1 cut(s) 291
BbsI GAAGAC 2 cut(s) 291, 1080
Bbv12I GWGCWC 1 cut(s) 1305
BbvCI CCTCAGC 1 cut(s) 491
BbvI GCAGC 3 cut(s) 202, 331, 1221
BccI CCATC 2 cut(s) 464, 1065
BceAI ACGGC 2 cut(s) 73, 493
BclI TGATCA 2 cut(s) 88, 252
BcoDI GTCTC 4 cut(s) 668, 755, 847, 1349
BfaI CTAG 1 cut(s) 957
BfrI CTTAAG 1 cut(s) 1283
BfuAI ACCTGC 1 cut(s) 1219
BisI GCNGC 5 cut(s) 216, 320, 408, 519, 1235
BlpI GCTNAGC 1 cut(s) 895
BlsI GCNGC 5 cut(s) 217, 321, 409, 520, 1236
Bme18I GGWCC 1 cut(s) 527
BmeT110I CYCGRG 1 cut(s) 1331
BmgT120I GGNCC 3 cut(s) 473, 527, 1443
BmiI GGNNCC 3 cut(s) 293, 475, 1014
BmsI GCATC 3 cut(s) 160, 477, 1189
BpiI GAAGAC 2 cut(s) 291, 1080
BpmI CTGGAG 2 cut(s) 460, 1031
Bpu10I CCTNAGC 1 cut(s) 491
Bpu1102I GCTNAGC 1 cut(s) 895
Bpu14I TTCGAA 2 cut(s) 942, 1091
Bsa29I ATCGAT 1 cut(s) 395
BsaBI GATNNNNATC 2 cut(s) 87, 1050
BsaI GGTCTC 2 cut(s) 668, 847
BsaJI CCNNGG 2 cut(s) 871, 1479
BsaWI WCCGGW 2 cut(s) 637, 748
Bsc4I CCNNNNNNNGG 4 cut(s) 301, 720, 751, 1498
Bse1I ACTGG 1 cut(s) 248
Bse21I CCTNAGG 1 cut(s) 1269
Bse8I GATNNNNATC 2 cut(s) 87, 1050
BseAI TCCGGA 1 cut(s) 637
BseCI ATCGAT 1 cut(s) 395
BseDI CCNNGG 2 cut(s) 871, 1479
BseGI GGATG 4 cut(s) 10, 175, 475, 1279
BseJI GATNNNNATC 2 cut(s) 87, 1050
BseLI CCNNNNNNNGG 4 cut(s) 301, 720, 751, 1498
BseMII CTCAG 7 cut(s) 171, 248, 482, 909, 1164, 1260, 1374
BseNI ACTGG 1 cut(s) 248
BseRI GAGGAG 2 cut(s) 749, 1133
BseX3I CGGCCG 1 cut(s) 405
BseXI GCAGC 3 cut(s) 202, 331, 1221
Bsh1236I CGCG 2 cut(s) 479, 971
Bsh1285I CGRYCG 1 cut(s) 408
BshFI GGCC 4 cut(s) 407, 474, 1445, 1491
BshVI ATCGAT 1 cut(s) 395
BsiEI CGRYCG 1 cut(s) 408
BsiHKAI GWGCWC 1 cut(s) 1305
BsiHKCI CYCGRG 1 cut(s) 1331
BsiSI CCGG 4 cut(s) 295, 638, 749, 1016
BsiWI CGTACG 1 cut(s) 597
BslFI GGGAC 1 cut(s) 483
BslI CCNNNNNNNGG 4 cut(s) 301, 720, 751, 1498
BsmAI GTCTC 4 cut(s) 668, 755, 847, 1349
BsmBI CGTCTC 1 cut(s) 755
BsmFI GGGAC 1 cut(s) 483
BsnI GGCC 4 cut(s) 407, 474, 1445, 1491
Bso31I GGTCTC 2 cut(s) 668, 847
BsoBI CYCGRG 1 cut(s) 1331
Bsp119I TTCGAA 2 cut(s) 942, 1091
Bsp1286I GDGCHC 1 cut(s) 1305
Bsp13I TCCGGA 1 cut(s) 637
Bsp1720I GCTNAGC 1 cut(s) 895
BspANI GGCC 4 cut(s) 407, 474, 1445, 1491
BspCNI CTCAG 7 cut(s) 172, 247, 483, 908, 1165, 1261, 1375
BspDI ATCGAT 1 cut(s) 395
BspEI TCCGGA 1 cut(s) 637
BspFNI CGCG 2 cut(s) 479, 971
BspLI GGNNCC 3 cut(s) 293, 475, 1014
BspMI ACCTGC 1 cut(s) 1219
BspPI GGATC 4 cut(s) 286, 299, 400, 1026
BspT104I TTCGAA 2 cut(s) 942, 1091
BspTI CTTAAG 1 cut(s) 1283
BspTNI GGTCTC 2 cut(s) 668, 847
BsrBI CCGCTC 1 cut(s) 571
BsrI ACTGG 1 cut(s) 248
BssECI CCNNGG 2 cut(s) 871, 1479
BssT1I CCWWGG 2 cut(s) 871, 1479
Bst4CI ACNGT 5 cut(s) 133, 746, 758, 766, 1164
Bst6I CTCTTC 2 cut(s) 96, 141
BstAFI CTTAAG 1 cut(s) 1283
BstAPI GCANNNNNTGC 1 cut(s) 1234
BstBI TTCGAA 2 cut(s) 942, 1091
BstC8I GCNNGC 3 cut(s) 220, 412, 800
BstDEI CTNAG 7 cut(s) 180, 234, 491, 895, 1173, 1269, 1383
BstENI CCTNNNNNAGG 1 cut(s) 1496
BstF5I GGATG 4 cut(s) 10, 175, 475, 1279
BstFNI CGCG 2 cut(s) 479, 971
BstHHI GCGC 2 cut(s) 811, 1352
BstMAI GTCTC 4 cut(s) 668, 755, 847, 1349
BstMCI CGRYCG 1 cut(s) 408
BstMWI GCNNNNNNNGC 4 cut(s) 577, 808, 977, 1234
BstNSI RCATGY 2 cut(s) 22, 1267
BstUI CGCG 2 cut(s) 479, 971
BstV1I GCAGC 3 cut(s) 202, 331, 1221
BstV2I GAAGAC 2 cut(s) 291, 1080
BstX2I RGATCY 1 cut(s) 291
BstYI RGATCY 1 cut(s) 291
BstZI CGGCCG 1 cut(s) 405
Bsu15I ATCGAT 1 cut(s) 395
Bsu36I CCTNAGG 1 cut(s) 1269
BsuRI GGCC 4 cut(s) 407, 474, 1445, 1491
BsuTUI ATCGAT 1 cut(s) 395
BtgZI GCGATG 1 cut(s) 1084
BtsCI GGATG 4 cut(s) 10, 175, 475, 1279
BtsI GCAGTG 1 cut(s) 818
BtsIMutI CAGTG 3 cut(s) 129, 177, 818
BveI ACCTGC 1 cut(s) 1219
Cac8I GCNNGC 3 cut(s) 220, 412, 800
CaiI CAGNNNCTG 2 cut(s) 1168, 1234
CfoI GCGC 2 cut(s) 811, 1352
Cfr13I GGNCC 3 cut(s) 473, 527, 1443
ClaI ATCGAT 1 cut(s) 395
CseI GACGC 1 cut(s) 1268
Csp6I GTAC 8 cut(s) 22, 194, 565, 574, 598, 649, 729, 1324
CviAII CATG 5 cut(s) 19, 1203, 1264, 1316, 1409
CviQI GTAC 8 cut(s) 22, 194, 565, 574, 598, 649, 729, 1324
DdeI CTNAG 7 cut(s) 180, 234, 491, 895, 1173, 1269, 1383
EaeI YGGCCR 1 cut(s) 405
EagI CGGCCG 1 cut(s) 405
Eam1104I CTCTTC 2 cut(s) 96, 141
EarI CTCTTC 2 cut(s) 96, 141
EclXI CGGCCG 1 cut(s) 405
Eco130I CCWWGG 2 cut(s) 871, 1479
Eco31I GGTCTC 2 cut(s) 668, 847
Eco47I GGWCC 1 cut(s) 527
Eco52I CGGCCG 1 cut(s) 405
Eco57I CTGAAG 2 cut(s) 1016, 1052
Eco81I CCTNAGG 1 cut(s) 1269
Eco88I CYCGRG 1 cut(s) 1331
EcoNI CCTNNNNNAGG 1 cut(s) 1496
EcoT14I CCWWGG 2 cut(s) 871, 1479
EcoT22I ATGCAT 2 cut(s) 1204, 1414
ErhI CCWWGG 2 cut(s) 871, 1479
Esp3I CGTCTC 1 cut(s) 755
FaeI CATG 5 cut(s) 22, 1206, 1267, 1319, 1412
FalI AAGNNNNNCTT 2 cut(s) 1388, 1420
FaqI GGGAC 1 cut(s) 483
FatI CATG 5 cut(s) 18, 1202, 1263, 1315, 1408
FauI CCCGC 1 cut(s) 484
FauNDI CATATG 1 cut(s) 885
FbaI TGATCA 2 cut(s) 88, 252
Fnu4HI GCNGC 5 cut(s) 216, 320, 408, 519, 1235
FokI GGATG 4 cut(s) 17, 182, 482, 1286
Fsp4HI GCNGC 5 cut(s) 216, 320, 408, 519, 1235
FspBI CTAG 1 cut(s) 957
FspI TGCGCA 1 cut(s) 810
GlaI GCGC 2 cut(s) 810, 1351
GluI GCNGC 5 cut(s) 216, 320, 408, 519, 1235
GsuI CTGGAG 2 cut(s) 460, 1031
HaeIII GGCC 4 cut(s) 407, 474, 1445, 1491
HapII CCGG 4 cut(s) 295, 638, 749, 1016
HgaI GACGC 1 cut(s) 1268
HhaI GCGC 2 cut(s) 811, 1352
Hin1II CATG 5 cut(s) 22, 1206, 1267, 1319, 1412
Hin6I GCGC 2 cut(s) 809, 1350
HinP1I GCGC 2 cut(s) 809, 1350
HincII GTYRAC 1 cut(s) 826
HindII GTYRAC 1 cut(s) 826
HindIII AAGCTT 1 cut(s) 158
HinfI GANTC 4 cut(s) 107, 739, 859, 1109
HpaI GTTAAC 1 cut(s) 826
HpaII CCGG 4 cut(s) 295, 638, 749, 1016
HphI GGTGA 4 cut(s) 734, 778, 872, 1135
Hpy166II GTNNAC 3 cut(s) 754, 826, 1504
Hpy188I TCNGA 7 cut(s) 138, 342, 864, 1023, 1174, 1384, 1498
Hpy188III TCNNGA 6 cut(s) 164, 500, 530, 638, 1333, 1462
Hpy8I GTNNAC 3 cut(s) 754, 826, 1504
Hpy99I CGWCG 8 cut(s) 50, 53, 56, 59, 80, 83, 269, 605
HpyAV CCTTC 4 cut(s) 675, 991, 1027, 1087
HpyCH4III ACNGT 5 cut(s) 133, 746, 758, 766, 1164
HpyCH4IV ACGT 2 cut(s) 249, 600
HpyCH4V TGCA 6 cut(s) 849, 1202, 1309, 1319, 1366, 1412
HpyF10VI GCNNNNNNNGC 4 cut(s) 577, 808, 977, 1234
HpyF3I CTNAG 7 cut(s) 180, 234, 491, 895, 1173, 1269, 1383
HpySE526I ACGT 2 cut(s) 249, 600
Hsp92II CATG 5 cut(s) 22, 1206, 1267, 1319, 1412
HspAI GCGC 2 cut(s) 809, 1350
Kpn2I TCCGGA 1 cut(s) 637
Ksp22I TGATCA 2 cut(s) 88, 252
KspAI GTTAAC 1 cut(s) 826
LmnI GCTCC 6 cut(s) 316, 640, 904, 1012, 1144, 1242
Lsp1109I GCAGC 3 cut(s) 202, 331, 1221
LweI GCATC 3 cut(s) 160, 477, 1189
MaeI CTAG 1 cut(s) 957
MaeII ACGT 2 cut(s) 249, 600
MaeIII GTNAC 6 cut(s) 71, 446, 740, 878, 965, 1471
MbiI CCGCTC 1 cut(s) 571
MflI RGATCY 1 cut(s) 291
MhlI GDGCHC 1 cut(s) 1305
MluCI AATT 7 cut(s) 332, 724, 938, 1186, 1215, 1361, 1391
MluI ACGCGT 1 cut(s) 969
MlyI GAGTC 2 cut(s) 748, 1118
MmeI TCCRAC 1 cut(s) 294
Mph1103I ATGCAT 2 cut(s) 1204, 1414
MroI TCCGGA 1 cut(s) 637
MseI TTAA 4 cut(s) 825, 1284, 1394, 1421
MslI CAYNNNNRTG 2 cut(s) 182, 1062
MspA1I CMGCKG 1 cut(s) 215
MspCI CTTAAG 1 cut(s) 1283
MspI CCGG 4 cut(s) 295, 638, 749, 1016
MvnI CGCG 2 cut(s) 479, 971
MwoI GCNNNNNNNGC 4 cut(s) 577, 808, 977, 1234
NdeI CATATG 1 cut(s) 885
NlaIII CATG 5 cut(s) 22, 1206, 1267, 1319, 1412
NlaIV GGNNCC 3 cut(s) 293, 475, 1014
NmeAIII GCCGAG 1 cut(s) 1378
NmuCI GTSAC 3 cut(s) 71, 740, 878
NsbI TGCGCA 1 cut(s) 810
NsiI ATGCAT 2 cut(s) 1204, 1414
NspI RCATGY 2 cut(s) 22, 1267
NspV TTCGAA 2 cut(s) 942, 1091
OliI CACNNNNGTG 1 cut(s) 182
PaeR7I CTCGAG 1 cut(s) 1331
PaqCI CACCTGC 1 cut(s) 1219
PciI ACATGT 1 cut(s) 18
PcsI WCGNNNNNNNCGW 3 cut(s) 30, 51, 63
PfeI GAWTC 2 cut(s) 107, 859
Pfl23II CGTACG 1 cut(s) 597
PkrI GCNGC 5 cut(s) 217, 321, 409, 520, 1236
PleI GAGTC 2 cut(s) 747, 1117
PpsI GAGTC 2 cut(s) 747, 1117
PscI ACATGT 1 cut(s) 18
PspLI CGTACG 1 cut(s) 597
PspN4I GGNNCC 3 cut(s) 293, 475, 1014
PspPI GGNCC 3 cut(s) 473, 527, 1443
PstNI CAGNNNCTG 2 cut(s) 1168, 1234
PsuI RGATCY 1 cut(s) 291
RsaI GTAC 8 cut(s) 23, 195, 566, 575, 599, 650, 730, 1325
RsaNI GTAC 8 cut(s) 22, 194, 565, 574, 598, 649, 729, 1324
RseI CAYNNNNRTG 2 cut(s) 182, 1062
SaqAI TTAA 4 cut(s) 825, 1284, 1394, 1421
SatI GCNGC 5 cut(s) 216, 320, 408, 519, 1235
Sau96I GGNCC 3 cut(s) 473, 527, 1443
SchI GAGTC 2 cut(s) 748, 1118
SduI GDGCHC 1 cut(s) 1305
SfaNI GCATC 3 cut(s) 160, 477, 1189
Sfr274I CTCGAG 1 cut(s) 1331
SfuI TTCGAA 2 cut(s) 942, 1091
SinI GGWCC 1 cut(s) 527
SlaI CTCGAG 1 cut(s) 1331
SmiMI CAYNNNNRTG 2 cut(s) 182, 1062
SmlI CTYRAG 2 cut(s) 1283, 1331
SmoI CTYRAG 2 cut(s) 1283, 1331
Sse9I AATT 7 cut(s) 332, 724, 938, 1186, 1215, 1361, 1391
SspMI CTAG 1 cut(s) 957
StyI CCWWGG 2 cut(s) 871, 1479
TaaI ACNGT 5 cut(s) 133, 746, 758, 766, 1164
TaiI ACGT 2 cut(s) 252, 603
TaqI TCGA 9 cut(s) 95, 395, 554, 603, 942, 1091, 1107, 1332, 1428
TaqII GACCGA 2 cut(s) 532, 871
TasI AATT 7 cut(s) 332, 724, 938, 1186, 1215, 1361, 1391
TatI WGTACW 2 cut(s) 193, 1323
TauI GCSGC 2 cut(s) 410, 521
TfiI GAWTC 2 cut(s) 107, 859
Tru1I TTAA 4 cut(s) 825, 1284, 1394, 1421
Tru9I TTAA 4 cut(s) 825, 1284, 1394, 1421
TscAI CASTG 3 cut(s) 136, 184, 818
TseFI GTSAC 3 cut(s) 71, 740, 878
TseI GCWGC 3 cut(s) 215, 319, 1234
Tsp45I GTSAC 3 cut(s) 71, 740, 878
TspDTI ATGAA 4 cut(s) 297, 797, 1046, 1180
TspGWI ACGGA 1 cut(s) 746
TspRI CASTG 3 cut(s) 136, 184, 818
Vha464I CTTAAG 1 cut(s) 1283
VpaK11BI GGWCC 1 cut(s) 527
XagI CCTNNNNNAGG 1 cut(s) 1496
XapI RAATTY 1 cut(s) 938
XceI RCATGY 2 cut(s) 22, 1267
XcmI CCANNNNNNNNNTGG 1 cut(s) 1138
XhoI CTCGAG 1 cut(s) 1331
XspI CTAG 1 cut(s) 957
Zsp2I ATGCAT 2 cut(s) 1204, 1414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.