MD08G1038100.v1.1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0000864
Physical Location & Seq
Forward (+)
2802740 .. 2804347
1608 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1038100.v1.1.491

Sequence Viewer

Length: 1608 bp
ATGGACAAGAAACGCAACCTGAACGGAGACAACTACATCGTCATAAGCAGCGATGAAGAATCGGATCGATCAGAATGTTTTGAAGAAGAAGACGACGCAGTGGATGATGAGTTTGCTTTTGTCCAAGAAGACAACATCGACAACACCGATCACTACGAGTTCAGCGAAAATACTGATTCGTCTCATGATCCAAATCAAAACTACGCCATTTTGAAAGAGGAAGAGATTCGCCAACTGCAGGAGGAAGATATCACAACAGTTTCTACTATCCTTTCCATACCGAAATCCGCTGCATGCTTCCTCCTCTCACACTTCAACTGGAGCGTCGGCAAAGTCAGCGAGGAGTGGTTCGCCGACGAAGATAGAGTCAGAGAGAAGGTAGGGTTGTTGAGGAACCCCGTGGTTAGTTTCCATCCCACAGATTTGATTACTTGCAGAATATGTTTTGATGATCATCAGTATGGATCGAAGGATGTTTTTTTCGCTGTTTGCGGTCATCCCTTTTGTCGGGATTGCTGGACAGGTTATTTTAAATCAGCGATAGATGAGGGTCCGACGTGTTTGATGCTGAGATGTCCCGAACCGTCTTGCAAGGCAGCGGTTGGACCGGATTTAATCGAGGGTTTGACGACAGGAGATCATGAGAGGTATAAGTCCTTCCTTTTGAGATCTTACGTTGAAGGGCGCAGGGAGATCAAGTGGTGCCCTGCACCGGATTGCAAGTACGCTGTGAAATTCAATGCAGGTGGAACCAAAAGCTATGACGTTTCGTGCCTTTGCTTGTACGGGTTTTGCTGGAATTGCAGTGAGGAAATTCACCGTCCCGTGAACTGCAAGACTGTGGAAAATTGGATTTTGAAGAACAAGGACGACTCGCTGAATGCGCAGTGGATACTTGGGAATACCAAGCCTTGCCCCAAGTGCAAGAAACCAATCGAGAAGAACCAAGGGTGTAATCATATGACTTGCTTAAACCCTTGCAGGTATGAATTTTGCTGGTATTGCCTCGAGAAGTATACTGGACATAGATGCTGCAATGGATATAATAGGGCAACTGGTGAATATACTGGAACTGACACGGACAAGGTTGAAGCTGGGAGGGATAAAAAGAGGGCAAAGAGATACGTGGAGAAATACCTACATTATTATGAACGATGGGCAAACAATCTATCTTCGAAGAAGAAAGCAATAGAGGATTTACATAATGTGCAGAGCACGGACATTATGAAGCTTGCCGCCATACAATGTCAATCGGAGCTTCAGCTCAAGTTTATTGTAGAGGCGTGGCAGCAGATCATTGAATGTAGGCAGGTATTGCGATGGACGTATGCATACGGGTACTACATTCCCGAGAATGAAGAACTGAAGATTCAGCTATTCGAGCACTTGCAAGGCCACGCGGAGTTCAGCCTGGAGAGGCTTCACAAATGTGCTGAGAGTGAGATGAAAGAGTTTCTCTTTGCTAAAGGTCCGTTGGAAAGTTTCCAGGACTTTCGTATAAAGCTCGTAAGACTGACAAAGGTGACTGCAAGTTATTTTGAGAAGCTGGTTAGAGCGTTGGAGAATGATCTTTCTGAATTGGACATACAGGGGATTCACAGGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IBR zf-RanBP

Protein Analysis

536

Amino Acids

62.21

Weight (kDa)

5.37

Isoelectric Point (pI)

47.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UBA_ARI1 PF21235 78 - 117 9.2e-11 E3 ubiquitin-protein ligase ARIH1, UBA-like domain
IBR PF01485 215 - 278 1.6e-16 IBR domain, a half RING-finger domain
IBR_1 PF22191 301 - 342 9e-12 IBR domain
IBR_2 PF22605 301 - 340 2.1e-09 IBR domain
Ariadne PF19422 378 - 525 2.4e-06 Ariadne domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 734
Acc16I TGCGCA 1 cut(s) 885
Acc36I ACCTGC 3 cut(s) 734, 972, 1300
AccB1I GGYRCC 1 cut(s) 702
AccI GTMKAC 1 cut(s) 1016
AccII CGCG 1 cut(s) 1400
AciI CCGC 5 cut(s) 288, 492, 599, 1236, 1400
AclWI GGATC 3 cut(s) 72, 182, 472
AcsI RAATTY 3 cut(s) 734, 813, 989
AcuI CTGAAG 2 cut(s) 1244, 1385
AfaI GTAC 3 cut(s) 725, 785, 1340
AfiI CCNNNNNNNGG 3 cut(s) 238, 507, 712
AflIII ACRYGT 1 cut(s) 557
AgsI TTSAA 8 cut(s) 83, 214, 316, 680, 739, 859, 1091, 1301
AjiI CACGTC 1 cut(s) 558
AjnI CCWGG 2 cut(s) 1410, 1485
AleI CACNNNNGTG 1 cut(s) 1428
AluBI AGCT 8 cut(s) 759, 1094, 1231, 1258, 1264, 1375, 1504, 1546
AluI AGCT 8 cut(s) 759, 1094, 1231, 1258, 1264, 1375, 1504, 1546
Alw21I GWGCWC 2 cut(s) 1217, 1386
Alw26I GTCTC 2 cut(s) 21, 186
AlwI GGATC 3 cut(s) 72, 182, 472
Ama87I CYCGRG 2 cut(s) 1007, 1349
AoxI GGCC 1 cut(s) 1393
ApeKI GCWGC 5 cut(s) 48, 290, 596, 1032, 1288
ApoI RAATTY 3 cut(s) 734, 813, 989
ArsI GACNNNNNNTTYG 2 cut(s) 351, 383
Asp700I GAANNNNTTC 1 cut(s) 225
AspLEI GCGC 2 cut(s) 687, 886
AspS9I GGNCC 3 cut(s) 551, 605, 1469
AsuHPI GGTGA 3 cut(s) 809, 1070, 1534
AsuII TTCGAA 1 cut(s) 1175
AvaI CYCGRG 2 cut(s) 1007, 1349
AvaII GGWCC 3 cut(s) 551, 605, 1469
BaeGI GKGCMC 1 cut(s) 707
BanI GGYRCC 1 cut(s) 702
BarI GAAGNNNNNNTAC 2 cut(s) 641, 673
BbsI GAAGAC 2 cut(s) 96, 135
Bbv12I GWGCWC 2 cut(s) 1217, 1386
BbvI GCAGC 5 cut(s) 60, 277, 608, 1019, 1300
BccI CCATC 3 cut(s) 420, 1149, 1314
BciT130I CCWGG 2 cut(s) 1412, 1487
BciVI GTATCC 1 cut(s) 885
BclI TGATCA 1 cut(s) 451
BcoDI GTCTC 2 cut(s) 21, 186
BfmI CTRYAG 1 cut(s) 236
BfuAI ACCTGC 3 cut(s) 734, 972, 1300
BfuI GTATCC 1 cut(s) 885
BglII AGATCT 1 cut(s) 668
BisI GCNGC 6 cut(s) 49, 291, 597, 1033, 1236, 1289
BlsI GCNGC 6 cut(s) 50, 292, 598, 1034, 1237, 1290
Bme1390I CCNGG 2 cut(s) 1412, 1487
Bme18I GGWCC 3 cut(s) 551, 605, 1469
BmeT110I CYCGRG 2 cut(s) 1007, 1349
BmgBI CACGTC 1 cut(s) 558
BmgT120I GGNCC 3 cut(s) 551, 605, 1469
BmiI GGNNCC 4 cut(s) 395, 552, 704, 751
BmrFI CCNGG 2 cut(s) 1412, 1487
BmsI GCATC 2 cut(s) 555, 1019
BpiI GAAGAC 2 cut(s) 96, 135
BpmI CTGGAG 2 cut(s) 340, 1433
Bpu14I TTCGAA 1 cut(s) 1175
BpuEI CTTGAG 1 cut(s) 1250
Bsa29I ATCGAT 1 cut(s) 67
BsaAI YACGTR 1 cut(s) 1126
BsaBI GATNNNNATC 2 cut(s) 192, 453
BsaJI CCNNGG 2 cut(s) 399, 946
BsaWI WCCGGW 2 cut(s) 607, 712
BsaXI ACNNNNNCTCC 2 cut(s) 683, 713
Bsc4I CCNNNNNNNGG 3 cut(s) 238, 507, 712
Bse1I ACTGG 4 cut(s) 323, 1024, 1060, 1072
Bse3DI GCAATG 1 cut(s) 1042
Bse8I GATNNNNATC 2 cut(s) 192, 453
BseBI CCWGG 2 cut(s) 1412, 1487
BseCI ATCGAT 1 cut(s) 67
BseDI CCNNGG 2 cut(s) 399, 946
BseGI GGATG 4 cut(s) 109, 412, 478, 496
BseJI GATNNNNATC 2 cut(s) 192, 453
BseLI CCNNNNNNNGG 3 cut(s) 238, 507, 712
BseMI GCAATG 1 cut(s) 1042
BseMII CTCAG 2 cut(s) 560, 1425
BseNI ACTGG 4 cut(s) 323, 1024, 1060, 1072
BseRI GAGGAG 2 cut(s) 293, 356
BseSI GKGCMC 1 cut(s) 707
BseXI GCAGC 5 cut(s) 60, 277, 608, 1019, 1300
BseYI CCCAGC 1 cut(s) 1094
BsgI GTGCAG 2 cut(s) 693, 1229
Bsh1236I CGCG 1 cut(s) 1400
BshFI GGCC 1 cut(s) 1395
BshNI GGYRCC 1 cut(s) 702
BshVI ATCGAT 1 cut(s) 67
BsiHKAI GWGCWC 2 cut(s) 1217, 1386
BsiHKCI CYCGRG 2 cut(s) 1007, 1349
BsiSI CCGG 2 cut(s) 608, 713
BslFI GGGAC 2 cut(s) 561, 807
BslI CCNNNNNNNGG 3 cut(s) 238, 507, 712
BsmAI GTCTC 2 cut(s) 21, 186
BsmBI CGTCTC 1 cut(s) 186
BsmFI GGGAC 2 cut(s) 561, 807
BsmI GAATGC 1 cut(s) 886
BsnI GGCC 1 cut(s) 1395
BsoBI CYCGRG 2 cut(s) 1007, 1349
Bsp119I TTCGAA 1 cut(s) 1175
Bsp1286I GDGCHC 3 cut(s) 707, 1217, 1386
BspACI CCGC 5 cut(s) 288, 492, 599, 1236, 1400
BspANI GGCC 1 cut(s) 1395
BspCNI CTCAG 2 cut(s) 561, 1426
BspDI ATCGAT 1 cut(s) 67
BspFNI CGCG 1 cut(s) 1400
BspHI TCATGA 2 cut(s) 184, 640
BspLI GGNNCC 4 cut(s) 395, 552, 704, 751
BspMAI CTGCAG 1 cut(s) 240
BspMI ACCTGC 3 cut(s) 734, 972, 1300
BspPI GGATC 3 cut(s) 72, 182, 472
BspT104I TTCGAA 1 cut(s) 1175
BspT107I GGYRCC 1 cut(s) 702
BsrDI GCAATG 1 cut(s) 1042
BsrI ACTGG 4 cut(s) 323, 1024, 1060, 1072
BssECI CCNNGG 2 cut(s) 399, 946
BssNAI GTATAC 1 cut(s) 1017
BssT1I CCWWGG 1 cut(s) 946
Bst1107I GTATAC 1 cut(s) 1017
Bst2UI CCWGG 2 cut(s) 1412, 1487
Bst4CI ACNGT 4 cut(s) 259, 585, 821, 841
Bst6I CTCTTC 1 cut(s) 216
BstAPI GCANNNNNTGC 1 cut(s) 1315
BstBAI YACGTR 1 cut(s) 1126
BstBI TTCGAA 1 cut(s) 1175
BstC8I GCNNGC 2 cut(s) 295, 1233
BstDEI CTNAG 2 cut(s) 569, 1434
BstDSI CCRYGG 1 cut(s) 399
BstF5I GGATG 4 cut(s) 109, 412, 478, 496
BstFNI CGCG 1 cut(s) 1400
BstHHI GCGC 2 cut(s) 687, 886
BstMAI GTCTC 2 cut(s) 21, 186
BstMWI GCNNNNNNNGC 7 cut(s) 336, 801, 883, 921, 1002, 1315, 1381
BstNI CCWGG 2 cut(s) 1412, 1487
BstNSI RCATGY 1 cut(s) 297
BstSCI CCNGG 2 cut(s) 1410, 1485
BstSFI CTRYAG 1 cut(s) 236
BstSLI GKGCMC 1 cut(s) 707
BstUI CGCG 1 cut(s) 1400
BstV1I GCAGC 5 cut(s) 60, 277, 608, 1019, 1300
BstV2I GAAGAC 2 cut(s) 96, 135
BstX2I RGATCY 1 cut(s) 668
BstYI RGATCY 1 cut(s) 668
BstZ17I GTATAC 1 cut(s) 1017
Bsu15I ATCGAT 1 cut(s) 67
BsuI GTATCC 1 cut(s) 885
BsuRI GGCC 1 cut(s) 1395
BsuTUI ATCGAT 1 cut(s) 67
BtgI CCRYGG 1 cut(s) 399
BtgZI GCGATG 2 cut(s) 66, 1333
BtrI CACGTC 1 cut(s) 558
BtsCI GGATG 4 cut(s) 109, 412, 478, 496
BtsI GCAGTG 3 cut(s) 105, 811, 893
BtsIMutI CAGTG 3 cut(s) 105, 811, 893
BveI ACCTGC 3 cut(s) 734, 972, 1300
Cac8I GCNNGC 2 cut(s) 295, 1233
CciI TCATGA 2 cut(s) 184, 640
CfoI GCGC 2 cut(s) 687, 886
Cfr13I GGNCC 3 cut(s) 551, 605, 1469
ClaI ATCGAT 1 cut(s) 67
CseI GACGC 2 cut(s) 104, 313
Csp6I GTAC 3 cut(s) 724, 784, 1339
CspCI CAANNNNNGTGG 2 cut(s) 727, 762
CviAII CATG 3 cut(s) 185, 294, 641
CviQI GTAC 3 cut(s) 724, 784, 1339
DdeI CTNAG 2 cut(s) 569, 1434
DraI TTTAAA 1 cut(s) 532
Eam1104I CTCTTC 1 cut(s) 216
EarI CTCTTC 1 cut(s) 216
Eco130I CCWWGG 1 cut(s) 946
Eco32I GATATC 1 cut(s) 250
Eco47I GGWCC 3 cut(s) 551, 605, 1469
Eco57I CTGAAG 2 cut(s) 1244, 1385
Eco88I CYCGRG 2 cut(s) 1007, 1349
EcoRII CCWGG 2 cut(s) 1410, 1485
EcoRV GATATC 1 cut(s) 250
EcoT14I CCWWGG 1 cut(s) 946
EcoT22I ATGCAT 1 cut(s) 1333
ErhI CCWWGG 1 cut(s) 946
Esp3I CGTCTC 1 cut(s) 186
FaeI CATG 3 cut(s) 188, 297, 644
FaqI GGGAC 2 cut(s) 561, 807
FatI CATG 3 cut(s) 184, 293, 640
FauNDI CATATG 1 cut(s) 960
FbaI TGATCA 1 cut(s) 451
FblI GTMKAC 1 cut(s) 1016
Fnu4HI GCNGC 6 cut(s) 49, 291, 597, 1033, 1236, 1289
FokI GGATG 4 cut(s) 116, 399, 483, 485
Fsp4HI GCNGC 6 cut(s) 49, 291, 597, 1033, 1236, 1289
FspI TGCGCA 1 cut(s) 885
GlaI GCGC 2 cut(s) 686, 885
GluI GCNGC 6 cut(s) 49, 291, 597, 1033, 1236, 1289
GsaI CCCAGC 1 cut(s) 1098
GsuI CTGGAG 2 cut(s) 340, 1433
HaeIII GGCC 1 cut(s) 1395
HapII CCGG 2 cut(s) 608, 713
HgaI GACGC 2 cut(s) 104, 313
HhaI GCGC 2 cut(s) 687, 886
Hin1II CATG 3 cut(s) 188, 297, 644
Hin6I GCGC 2 cut(s) 685, 884
HinP1I GCGC 2 cut(s) 685, 884
HindIII AAGCTT 1 cut(s) 1229
HinfI GANTC 7 cut(s) 59, 176, 226, 366, 872, 1369, 1594
HpaII CCGG 2 cut(s) 608, 713
HphI GGTGA 3 cut(s) 809, 1070, 1534
Hpy166II GTNNAC 2 cut(s) 829, 1017
Hpy188I TCNGA 6 cut(s) 64, 73, 371, 555, 1255, 1576
Hpy188III TCNNGA 8 cut(s) 185, 509, 578, 641, 937, 1009, 1349, 1605
Hpy8I GTNNAC 2 cut(s) 829, 1017
Hpy99I CGWCG 4 cut(s) 98, 329, 359, 559
HpyAV CCTTC 4 cut(s) 370, 463, 667, 674
HpyCH4III ACNGT 4 cut(s) 259, 585, 821, 841
HpyCH4IV ACGT 5 cut(s) 557, 675, 765, 1125, 1325
HpyF10VI GCNNNNNNNGC 7 cut(s) 336, 801, 883, 921, 1002, 1315, 1381
HpyF3I CTNAG 2 cut(s) 569, 1434
HpySE526I ACGT 5 cut(s) 557, 675, 765, 1125, 1325
Hsp92II CATG 3 cut(s) 188, 297, 644
HspAI GCGC 2 cut(s) 685, 884
Ksp22I TGATCA 1 cut(s) 451
LmnI GCTCC 2 cut(s) 321, 1255
Lsp1109I GCAGC 5 cut(s) 60, 277, 608, 1019, 1300
LweI GCATC 2 cut(s) 555, 1019
MaeII ACGT 5 cut(s) 557, 675, 765, 1125, 1325
MaeIII GTNAC 1 cut(s) 1522
MflI RGATCY 1 cut(s) 668
MhlI GDGCHC 3 cut(s) 707, 1217, 1386
MluCI AATT 6 cut(s) 734, 799, 813, 847, 989, 1577
MlyI GAGTC 2 cut(s) 375, 866
MmeI TCCRAC 4 cut(s) 578, 583, 1455, 1539
Mph1103I ATGCAT 1 cut(s) 1333
MroXI GAANNNNTTC 1 cut(s) 225
MseI TTAA 3 cut(s) 531, 614, 971
MslI CAYNNNNRTG 3 cut(s) 459, 1146, 1428
MspA1I CMGCKG 2 cut(s) 290, 599
MspI CCGG 2 cut(s) 608, 713
MspR9I CCNGG 2 cut(s) 1412, 1487
Mva1269I GAATGC 1 cut(s) 886
MvaI CCWGG 2 cut(s) 1412, 1487
MvnI CGCG 1 cut(s) 1400
MwoI GCNNNNNNNGC 7 cut(s) 336, 801, 883, 921, 1002, 1315, 1381
NdeI CATATG 1 cut(s) 960
NlaIII CATG 3 cut(s) 188, 297, 644
NlaIV GGNNCC 4 cut(s) 395, 552, 704, 751
NmuCI GTSAC 1 cut(s) 1522
NsbI TGCGCA 1 cut(s) 885
NsiI ATGCAT 1 cut(s) 1333
NspI RCATGY 1 cut(s) 297
NspV TTCGAA 1 cut(s) 1175
OliI CACNNNNGTG 1 cut(s) 1428
PaeI GCATGC 1 cut(s) 297
PaeR7I CTCGAG 1 cut(s) 1007
PagI TCATGA 2 cut(s) 184, 640
PaqCI CACCTGC 1 cut(s) 734
PcsI WCGNNNNNNNCGW 2 cut(s) 144, 162
PctI GAATGC 1 cut(s) 886
PdmI GAANNNNTTC 1 cut(s) 225
PfeI GAWTC 5 cut(s) 59, 176, 226, 1369, 1594
PfoI TCCNGGA 1 cut(s) 1485
PkrI GCNGC 6 cut(s) 50, 292, 598, 1034, 1237, 1290
PleI GAGTC 2 cut(s) 374, 866
PpsI GAGTC 2 cut(s) 374, 866
Ppu21I YACGTR 1 cut(s) 1126
Psp6I CCWGG 2 cut(s) 1410, 1485
PspFI CCCAGC 1 cut(s) 1094
PspGI CCWGG 2 cut(s) 1410, 1485
PspN4I GGNNCC 4 cut(s) 395, 552, 704, 751
PspPI GGNCC 3 cut(s) 551, 605, 1469
PstI CTGCAG 1 cut(s) 240
PsuI RGATCY 1 cut(s) 668
RsaI GTAC 3 cut(s) 725, 785, 1340
RsaNI GTAC 3 cut(s) 724, 784, 1339
RseI CAYNNNNRTG 3 cut(s) 459, 1146, 1428
SaqAI TTAA 3 cut(s) 531, 614, 971
SatI GCNGC 6 cut(s) 49, 291, 597, 1033, 1236, 1289
Sau96I GGNCC 3 cut(s) 551, 605, 1469
SchI GAGTC 2 cut(s) 375, 866
ScrFI CCNGG 2 cut(s) 1412, 1487
SduI GDGCHC 3 cut(s) 707, 1217, 1386
SfaNI GCATC 2 cut(s) 555, 1019
SfcI CTRYAG 1 cut(s) 236
Sfr274I CTCGAG 1 cut(s) 1007
SfuI TTCGAA 1 cut(s) 1175
SinI GGWCC 3 cut(s) 551, 605, 1469
SlaI CTCGAG 1 cut(s) 1007
SmiMI CAYNNNNRTG 3 cut(s) 459, 1146, 1428
SmlI CTYRAG 2 cut(s) 1007, 1265
SmoI CTYRAG 2 cut(s) 1007, 1265
SphI GCATGC 1 cut(s) 297
Sse9I AATT 6 cut(s) 734, 799, 813, 847, 989, 1577
SsiI CCGC 5 cut(s) 288, 492, 599, 1236, 1400
StyD4I CCNGG 2 cut(s) 1410, 1485
StyI CCWWGG 1 cut(s) 946
TaaI ACNGT 4 cut(s) 259, 585, 821, 841
TaiI ACGT 5 cut(s) 560, 678, 768, 1128, 1328
TaqI TCGA 8 cut(s) 67, 138, 467, 618, 936, 1008, 1175, 1380
TasI AATT 6 cut(s) 734, 799, 813, 847, 989, 1577
TauI GCSGC 1 cut(s) 1238
TfiI GAWTC 5 cut(s) 59, 176, 226, 1369, 1594
Tru1I TTAA 3 cut(s) 531, 614, 971
Tru9I TTAA 3 cut(s) 531, 614, 971
TscAI CASTG 3 cut(s) 105, 811, 893
TseFI GTSAC 1 cut(s) 1522
TseI GCWGC 5 cut(s) 48, 290, 596, 1032, 1288
Tsp45I GTSAC 1 cut(s) 1522
TspDTI ATGAA 6 cut(s) 69, 1002, 1164, 1241, 1371, 1460
TspGWI ACGGA 4 cut(s) 39, 1094, 1232, 1461
TspRI CASTG 3 cut(s) 105, 811, 893
VpaK11BI GGWCC 3 cut(s) 551, 605, 1469
XapI RAATTY 3 cut(s) 734, 813, 989
XceI RCATGY 1 cut(s) 297
XhoI CTCGAG 1 cut(s) 1007
XmiI GTMKAC 1 cut(s) 1016
XmnI GAANNNNTTC 1 cut(s) 225
Zsp2I ATGCAT 1 cut(s) 1333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.