E3 ubiquitin-protein ligase
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 302 |
| AccB1I | GGYRCC | 1 cut(s) | 282 |
| AciI | CCGC | 2 cut(s) | 198, 240 |
| AclI | AACGTT | 1 cut(s) | 537 |
| AclWI | GGATC | 3 cut(s) | 16, 43, 586 |
| AfaI | GTAC | 3 cut(s) | 173, 185, 447 |
| AgsI | TTSAA | 5 cut(s) | 223, 380, 517, 667, 746 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 63 |
| AhlI | ACTAGT | 1 cut(s) | 59 |
| AluBI | AGCT | 3 cut(s) | 203, 237, 338 |
| AluI | AGCT | 3 cut(s) | 203, 237, 338 |
| AlwI | GGATC | 3 cut(s) | 16, 43, 586 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 25 |
| ApeKI | GCWGC | 2 cut(s) | 200, 237 |
| AsuHPI | GGTGA | 3 cut(s) | 305, 484, 688 |
| BanI | GGYRCC | 1 cut(s) | 282 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 118, 150 |
| BbvI | GCAGC | 2 cut(s) | 212, 224 |
| BccI | CCATC | 3 cut(s) | 503, 578, 677 |
| BcuI | ACTAGT | 1 cut(s) | 59 |
| BfaI | CTAG | 3 cut(s) | 60, 582, 760 |
| BisI | GCNGC | 2 cut(s) | 201, 238 |
| BlpI | GCTNAGC | 1 cut(s) | 396 |
| BlsI | GCNGC | 2 cut(s) | 202, 239 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 63 |
| BmiI | GGNNCC | 3 cut(s) | 284, 462, 688 |
| BmrI | ACTGGG | 1 cut(s) | 622 |
| BmsI | GCATC | 2 cut(s) | 121, 251 |
| BmuI | ACTGGG | 1 cut(s) | 622 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 678, 710 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 396 |
| BsaJI | CCNNGG | 2 cut(s) | 178, 289 |
| Bse1I | ACTGG | 3 cut(s) | 142, 617, 678 |
| Bse3DI | GCAATG | 1 cut(s) | 21 |
| BseDI | CCNNGG | 2 cut(s) | 178, 289 |
| BseGI | GGATG | 1 cut(s) | 559 |
| BseMI | GCAATG | 1 cut(s) | 21 |
| BseMII | CTCAG | 2 cut(s) | 410, 411 |
| BseNI | ACTGG | 3 cut(s) | 142, 617, 678 |
| BseRI | GAGGAG | 1 cut(s) | 363 |
| BseXI | GCAGC | 2 cut(s) | 212, 224 |
| BseYI | CCCAGC | 1 cut(s) | 431 |
| BsgI | GTGCAG | 2 cut(s) | 87, 567 |
| BshNI | GGYRCC | 1 cut(s) | 282 |
| BslFI | GGGAC | 2 cut(s) | 202, 425 |
| BsmFI | GGGAC | 2 cut(s) | 202, 425 |
| Bsp1407I | TGTACA | 1 cut(s) | 171 |
| Bsp143I | GATC | 3 cut(s) | 21, 48, 578 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 396 |
| Bsp19I | CCATGG | 1 cut(s) | 289 |
| BspACI | CCGC | 2 cut(s) | 198, 240 |
| BspCNI | CTCAG | 2 cut(s) | 409, 412 |
| BspLI | GGNNCC | 3 cut(s) | 284, 462, 688 |
| BspPI | GGATC | 3 cut(s) | 16, 43, 586 |
| BspQI | GCTCTTC | 1 cut(s) | 41 |
| BspT107I | GGYRCC | 1 cut(s) | 282 |
| BsrDI | GCAATG | 1 cut(s) | 21 |
| BsrGI | TGTACA | 1 cut(s) | 171 |
| BsrI | ACTGG | 3 cut(s) | 142, 617, 678 |
| BssECI | CCNNGG | 2 cut(s) | 178, 289 |
| BssMI | GATC | 3 cut(s) | 21, 48, 578 |
| BssT1I | CCWWGG | 2 cut(s) | 178, 289 |
| Bst4CI | ACNGT | 2 cut(s) | 227, 565 |
| Bst6I | CTCTTC | 2 cut(s) | 41, 224 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 25 |
| BstAUI | TGTACA | 1 cut(s) | 171 |
| BstC8I | GCNNGC | 1 cut(s) | 402 |
| BstDEI | CTNAG | 4 cut(s) | 6, 206, 396, 420 |
| BstDSI | CCRYGG | 1 cut(s) | 289 |
| BstF5I | GGATG | 1 cut(s) | 559 |
| BstKTI | GATC | 3 cut(s) | 24, 51, 581 |
| BstMBI | GATC | 3 cut(s) | 21, 48, 578 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 11, 25, 401, 625 |
| BstNSI | RCATGY | 3 cut(s) | 110, 156, 249 |
| BstV1I | GCAGC | 2 cut(s) | 212, 224 |
| BstX2I | RGATCY | 2 cut(s) | 21, 578 |
| BstYI | RGATCY | 2 cut(s) | 21, 578 |
| BtgI | CCRYGG | 1 cut(s) | 289 |
| BtgZI | GCGATG | 1 cut(s) | 274 |
| BtsCI | GGATG | 1 cut(s) | 559 |
| BtsI | GCAGTG | 2 cut(s) | 210, 306 |
| BtsIMutI | CAGTG | 5 cut(s) | 210, 232, 306, 570, 685 |
| Cac8I | GCNNGC | 1 cut(s) | 402 |
| CaiI | CAGNNNCTG | 1 cut(s) | 25 |
| Csp6I | GTAC | 3 cut(s) | 172, 184, 446 |
| CviAII | CATG | 5 cut(s) | 107, 153, 246, 290, 465 |
| CviJI | RGCY | 9 cut(s) | 5, 177, 203, 237, 338, 395, 400, 595, 689 |
| CviKI_1 | RGCY | 9 cut(s) | 5, 177, 203, 237, 338, 395, 400, 595, 689 |
| CviQI | GTAC | 3 cut(s) | 172, 184, 446 |
| DdeI | CTNAG | 4 cut(s) | 6, 206, 396, 420 |
| DpnI | GATC | 3 cut(s) | 23, 50, 580 |
| DpnII | GATC | 3 cut(s) | 21, 48, 578 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 302 |
| DriI | GACNNNNNGTC | 1 cut(s) | 63 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 302 |
| Eam1104I | CTCTTC | 2 cut(s) | 41, 224 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 63 |
| EarI | CTCTTC | 2 cut(s) | 41, 224 |
| Eco130I | CCWWGG | 2 cut(s) | 178, 289 |
| EcoT14I | CCWWGG | 2 cut(s) | 178, 289 |
| ErhI | CCWWGG | 2 cut(s) | 178, 289 |
| FaeI | CATG | 5 cut(s) | 110, 156, 249, 293, 468 |
| FaqI | GGGAC | 2 cut(s) | 202, 425 |
| FatI | CATG | 5 cut(s) | 106, 152, 245, 289, 464 |
| Fnu4HI | GCNGC | 2 cut(s) | 201, 238 |
| FokI | GGATG | 1 cut(s) | 566 |
| Fsp4HI | GCNGC | 2 cut(s) | 201, 238 |
| FspBI | CTAG | 3 cut(s) | 60, 582, 760 |
| GluI | GCNGC | 2 cut(s) | 201, 238 |
| GsaI | CCCAGC | 1 cut(s) | 435 |
| Hin1II | CATG | 5 cut(s) | 110, 156, 249, 293, 468 |
| HindIII | AAGCTT | 1 cut(s) | 336 |
| HinfI | GANTC | 1 cut(s) | 190 |
| HphI | GGTGA | 3 cut(s) | 305, 484, 688 |
| Hpy166II | GTNNAC | 1 cut(s) | 492 |
| Hpy188I | TCNGA | 1 cut(s) | 455 |
| Hpy188III | TCNNGA | 2 cut(s) | 377, 606 |
| Hpy8I | GTNNAC | 1 cut(s) | 492 |
| Hpy99I | CGWCG | 1 cut(s) | 272 |
| HpyAV | CCTTC | 3 cut(s) | 450, 673, 740 |
| HpyCH4III | ACNGT | 2 cut(s) | 227, 565 |
| HpyCH4IV | ACGT | 1 cut(s) | 537 |
| HpyCH4V | TGCA | 9 cut(s) | 19, 28, 104, 110, 152, 264, 366, 412, 548 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 11, 25, 401, 625 |
| HpyF3I | CTNAG | 4 cut(s) | 6, 206, 396, 420 |
| HpySE526I | ACGT | 1 cut(s) | 537 |
| Hsp92II | CATG | 5 cut(s) | 110, 156, 249, 293, 468 |
| Kzo9I | GATC | 3 cut(s) | 21, 48, 578 |
| LguI | GCTCTTC | 1 cut(s) | 41 |
| LmnI | GCTCC | 2 cut(s) | 242, 686 |
| Lsp1109I | GCAGC | 2 cut(s) | 212, 224 |
| LweI | GCATC | 2 cut(s) | 121, 251 |
| MaeI | CTAG | 3 cut(s) | 60, 582, 760 |
| MaeII | ACGT | 1 cut(s) | 537 |
| MaeIII | GTNAC | 4 cut(s) | 248, 293, 304, 503 |
| MalI | GATC | 3 cut(s) | 23, 50, 580 |
| MboI | GATC | 3 cut(s) | 21, 48, 578 |
| MboII | GAAGA | 3 cut(s) | 28, 211, 484 |
| MfeI | CAATTG | 2 cut(s) | 29, 254 |
| MflI | RGATCY | 2 cut(s) | 21, 578 |
| MluCI | AATT | 6 cut(s) | 29, 254, 622, 634, 728, 755 |
| MlyI | GAGTC | 1 cut(s) | 184 |
| MmeI | TCCRAC | 1 cut(s) | 369 |
| MnlI | CCTC | 7 cut(s) | 296, 329, 381, 384, 473, 653, 668 |
| MseI | TTAA | 1 cut(s) | 558 |
| MslI | CAYNNNNRTG | 1 cut(s) | 705 |
| MunI | CAATTG | 2 cut(s) | 29, 254 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 11, 25, 401, 625 |
| NcoI | CCATGG | 1 cut(s) | 289 |
| NdeII | GATC | 3 cut(s) | 21, 48, 578 |
| NlaIII | CATG | 5 cut(s) | 110, 156, 249, 293, 468 |
| NlaIV | GGNNCC | 3 cut(s) | 284, 462, 688 |
| NmuCI | GTSAC | 3 cut(s) | 293, 304, 503 |
| NspI | RCATGY | 3 cut(s) | 110, 156, 249 |
| PciSI | GCTCTTC | 1 cut(s) | 41 |
| PkrI | GCNGC | 2 cut(s) | 202, 239 |
| PleI | GAGTC | 1 cut(s) | 184 |
| PpsI | GAGTC | 1 cut(s) | 184 |
| Psp1406I | AACGTT | 1 cut(s) | 537 |
| PspFI | CCCAGC | 1 cut(s) | 431 |
| PspN4I | GGNNCC | 3 cut(s) | 284, 462, 688 |
| PstNI | CAGNNNCTG | 1 cut(s) | 25 |
| PsuI | RGATCY | 2 cut(s) | 21, 578 |
| RsaI | GTAC | 3 cut(s) | 173, 185, 447 |
| RsaNI | GTAC | 3 cut(s) | 172, 184, 446 |
| RseI | CAYNNNNRTG | 1 cut(s) | 705 |
| SapI | GCTCTTC | 1 cut(s) | 41 |
| SaqAI | TTAA | 1 cut(s) | 558 |
| SatI | GCNGC | 2 cut(s) | 201, 238 |
| Sau3AI | GATC | 3 cut(s) | 21, 48, 578 |
| SchI | GAGTC | 1 cut(s) | 184 |
| SfaNI | GCATC | 2 cut(s) | 121, 251 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 705 |
| SpeI | ACTAGT | 1 cut(s) | 59 |
| Sse9I | AATT | 6 cut(s) | 29, 254, 622, 634, 728, 755 |
| SsiI | CCGC | 2 cut(s) | 198, 240 |
| SspMI | CTAG | 3 cut(s) | 60, 582, 760 |
| StyI | CCWWGG | 2 cut(s) | 178, 289 |
| TaaI | ACNGT | 2 cut(s) | 227, 565 |
| TaiI | ACGT | 1 cut(s) | 540 |
| TaqI | TCGA | 1 cut(s) | 387 |
| TasI | AATT | 6 cut(s) | 29, 254, 622, 634, 728, 755 |
| TatI | WGTACW | 2 cut(s) | 171, 445 |
| Tru1I | TTAA | 1 cut(s) | 558 |
| Tru9I | TTAA | 1 cut(s) | 558 |
| TscAI | CASTG | 5 cut(s) | 217, 232, 313, 570, 685 |
| TseFI | GTSAC | 3 cut(s) | 293, 304, 503 |
| TseI | GCWGC | 2 cut(s) | 200, 237 |
| Tsp45I | GTSAC | 3 cut(s) | 293, 304, 503 |
| TspGWI | ACGGA | 1 cut(s) | 102 |
| TspRI | CASTG | 5 cut(s) | 217, 232, 313, 570, 685 |
| XceI | RCATGY | 3 cut(s) | 110, 156, 249 |
| XspI | CTAG | 3 cut(s) | 60, 582, 760 |