Rmu_co8320635.1_g000001

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8320635.1
OG0000864
Physical Location & Seq
Forward (+)
16 .. 702
687 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8320635.1_g000001.1.cds

Sequence Viewer

Length: 687 bp
atgagtgctgtcgagagccaaaagaagaaggcgaagatgtatttagagagataccttcattattttgaacgctgggacaacaatggaaaatcaaagcaaaaggctcttgaggattttgataaggtgaagaatgtgcacatacatgaacttggaaagatacagggaaatacaaaagagcatgaatacaatttcatcatagaggcctggcaacagataataatatgcaggcaagtactgcaatggacctatgcttatggctactacatgagtgatgatgagcctcgcaagagtcagctattcgagatcttgcaaggccatgccgagttcagcctcgagaggcttcacaagtgtgccgagagcgaactgcagcagtttcttgtctgtggtgataacaatgaccacttgctgaaaatttacggggattttagaaagagactcataaacctgacaaaggtgactggaacttattttgagaagctggctagtgcactggaggagggccttcctgagacgtgcccctatgatgaggctagttggacctgcgaccagtgtacttatatcaatgtggggactgccatcacctgtgacgtgtgtaatatggaacgtaatggttggtgttgcgacctctgtacttatcggaatccagcctctgccatgagctatgagatgtgttcttatgctgagtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IBR zf-RanBP

Protein Analysis

228

Amino Acids

26.74

Weight (kDa)

5.45

Isoelectric Point (pI)

48.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 548
AcsI RAATTY 1 cut(s) 411
AfaI GTAC 3 cut(s) 234, 553, 631
AfiI CCNNNNNNNGG 1 cut(s) 451
AflIII ACRYGT 1 cut(s) 588
AgsI TTSAA 1 cut(s) 68
AjiI CACGTC 2 cut(s) 513, 589
AjnI CCWGG 1 cut(s) 203
AleI CACNNNNGTG 1 cut(s) 348
AluBI AGCT 3 cut(s) 295, 478, 660
AluI AGCT 3 cut(s) 295, 478, 660
Alw21I GWGCWC 2 cut(s) 138, 490
Alw26I GTCTC 2 cut(s) 427, 503
Alw44I GTGCAC 2 cut(s) 134, 486
AlwNI CAGNNNCTG 1 cut(s) 650
Ama87I CYCGRG 1 cut(s) 332
AoxI GGCC 3 cut(s) 201, 313, 499
ApaLI GTGCAC 2 cut(s) 134, 486
ApeKI GCWGC 1 cut(s) 367
ApoI RAATTY 1 cut(s) 411
AspS9I GGNCC 3 cut(s) 243, 499, 537
AsuHPI GGTGA 4 cut(s) 136, 398, 466, 571
AvaI CYCGRG 1 cut(s) 332
AvaII GGWCC 2 cut(s) 243, 537
BaeGI GKGCMC 3 cut(s) 138, 490, 518
Bbv12I GWGCWC 2 cut(s) 138, 490
BbvI GCAGC 1 cut(s) 379
BccI CCATC 1 cut(s) 584
BciT130I CCWGG 1 cut(s) 205
BcoDI GTCTC 2 cut(s) 427, 503
BfaI CTAG 2 cut(s) 483, 531
BfmI CTRYAG 1 cut(s) 365
BfuAI ACCTGC 1 cut(s) 548
BglII AGATCT 1 cut(s) 303
BisI GCNGC 1 cut(s) 368
BlsI GCNGC 1 cut(s) 369
BmcAI AGTACT 1 cut(s) 234
Bme1390I CCNGG 1 cut(s) 205
Bme18I GGWCC 2 cut(s) 243, 537
BmeT110I CYCGRG 1 cut(s) 332
BmgBI CACGTC 2 cut(s) 513, 589
BmgT120I GGNCC 3 cut(s) 243, 499, 537
BmrFI CCNGG 1 cut(s) 205
BpmI CTGGAG 1 cut(s) 512
BpuEI CTTGAG 1 cut(s) 128
Bsc4I CCNNNNNNNGG 1 cut(s) 451
Bse1I ACTGG 3 cut(s) 463, 495, 547
Bse3DI GCAATG 1 cut(s) 245
BseBI CCWGG 1 cut(s) 205
BseLI CCNNNNNNNGG 1 cut(s) 451
BseMI GCAATG 1 cut(s) 245
BseMII CTCAG 2 cut(s) 498, 672
BseNI ACTGG 3 cut(s) 463, 495, 547
BseRI GAGGAG 1 cut(s) 509
BseSI GKGCMC 3 cut(s) 138, 490, 518
BseXI GCAGC 1 cut(s) 379
BseYI CCCAGC 1 cut(s) 72
BshFI GGCC 3 cut(s) 203, 315, 501
BsiHKAI GWGCWC 2 cut(s) 138, 490
BsiHKCI CYCGRG 1 cut(s) 332
BslFI GGGAC 2 cut(s) 89, 583
BslI CCNNNNNNNGG 1 cut(s) 451
BsmAI GTCTC 2 cut(s) 427, 503
BsmBI CGTCTC 1 cut(s) 503
BsmFI GGGAC 2 cut(s) 89, 583
BsnI GGCC 3 cut(s) 203, 315, 501
BsoBI CYCGRG 1 cut(s) 332
Bsp1286I GDGCHC 3 cut(s) 138, 490, 518
Bsp143I GATC 1 cut(s) 303
BspANI GGCC 3 cut(s) 203, 315, 501
BspCNI CTCAG 2 cut(s) 499, 673
BspMAI CTGCAG 1 cut(s) 369
BspMI ACCTGC 1 cut(s) 548
BsrDI GCAATG 1 cut(s) 245
BsrI ACTGG 3 cut(s) 463, 495, 547
BssMI GATC 1 cut(s) 303
Bst2UI CCWGG 1 cut(s) 205
BstAPI GCANNNNNTGC 1 cut(s) 235
BstC8I GCNNGC 2 cut(s) 227, 480
BstDEI CTNAG 2 cut(s) 507, 681
BstENI CCTNNNNNAGG 1 cut(s) 449
BstKTI GATC 1 cut(s) 306
BstMAI GTCTC 2 cut(s) 427, 503
BstMBI GATC 1 cut(s) 303
BstMWI GCNNNNNNNGC 1 cut(s) 235
BstNI CCWGG 1 cut(s) 205
BstSCI CCNGG 1 cut(s) 203
BstSFI CTRYAG 1 cut(s) 365
BstSLI GKGCMC 3 cut(s) 138, 490, 518
BstV1I GCAGC 1 cut(s) 379
BstX2I RGATCY 1 cut(s) 303
BstYI RGATCY 1 cut(s) 303
BsuRI GGCC 3 cut(s) 203, 315, 501
BtrI CACGTC 2 cut(s) 513, 589
BtsIMutI CAGTG 2 cut(s) 488, 554
BveI ACCTGC 1 cut(s) 548
Cac8I GCNNGC 2 cut(s) 227, 480
CaiI CAGNNNCTG 1 cut(s) 650
Cfr13I GGNCC 3 cut(s) 243, 499, 537
Csp6I GTAC 3 cut(s) 233, 552, 630
CviAII CATG 5 cut(s) 143, 179, 265, 317, 655
CviQI GTAC 3 cut(s) 233, 552, 630
DdeI CTNAG 2 cut(s) 507, 681
DpnI GATC 1 cut(s) 305
DpnII GATC 1 cut(s) 303
Eco147I AGGCCT 1 cut(s) 203
Eco47I GGWCC 2 cut(s) 243, 537
Eco88I CYCGRG 1 cut(s) 332
EcoNI CCTNNNNNAGG 1 cut(s) 449
EcoO109I RGGNCCY 1 cut(s) 499
EcoRII CCWGG 1 cut(s) 203
Esp3I CGTCTC 1 cut(s) 503
FaeI CATG 5 cut(s) 146, 182, 268, 320, 658
FaqI GGGAC 2 cut(s) 89, 583
FatI CATG 5 cut(s) 142, 178, 264, 316, 654
Fnu4HI GCNGC 1 cut(s) 368
Fsp4HI GCNGC 1 cut(s) 368
FspBI CTAG 2 cut(s) 483, 531
GluI GCNGC 1 cut(s) 368
GsaI CCCAGC 1 cut(s) 76
GsuI CTGGAG 1 cut(s) 512
HaeIII GGCC 3 cut(s) 203, 315, 501
Hin1II CATG 5 cut(s) 146, 182, 268, 320, 658
HinfI GANTC 3 cut(s) 289, 435, 640
HphI GGTGA 4 cut(s) 136, 398, 466, 571
Hpy166II GTNNAC 3 cut(s) 136, 488, 552
Hpy188I TCNGA 1 cut(s) 639
Hpy188III TCNNGA 5 cut(s) 13, 107, 301, 334, 506
Hpy8I GTNNAC 3 cut(s) 136, 488, 552
HpyAV CCTTC 3 cut(s) 22, 65, 512
HpyCH4IV ACGT 3 cut(s) 512, 588, 604
HpyCH4V TGCA 6 cut(s) 136, 225, 238, 310, 367, 488
HpyF10VI GCNNNNNNNGC 1 cut(s) 235
HpyF3I CTNAG 2 cut(s) 507, 681
HpySE526I ACGT 3 cut(s) 512, 588, 604
Hsp92II CATG 5 cut(s) 146, 182, 268, 320, 658
Kzo9I GATC 1 cut(s) 303
Lsp1109I GCAGC 1 cut(s) 379
MaeI CTAG 2 cut(s) 483, 531
MaeII ACGT 3 cut(s) 512, 588, 604
MaeIII GTNAC 2 cut(s) 454, 584
MalI GATC 1 cut(s) 305
MboI GATC 1 cut(s) 303
MboII GAAGA 3 cut(s) 37, 46, 139
MflI RGATCY 1 cut(s) 303
MhlI GDGCHC 3 cut(s) 138, 490, 518
MluCI AATT 2 cut(s) 187, 411
MlyI GAGTC 2 cut(s) 298, 429
MmeI TCCRAC 1 cut(s) 515
MslI CAYNNNNRTG 2 cut(s) 141, 348
MspR9I CCNGG 1 cut(s) 205
MvaI CCWGG 1 cut(s) 205
MwoI GCNNNNNNNGC 1 cut(s) 235
NdeII GATC 1 cut(s) 303
NlaIII CATG 5 cut(s) 146, 182, 268, 320, 658
NmeAIII GCCGAG 2 cut(s) 346, 379
NmuCI GTSAC 2 cut(s) 454, 584
OliI CACNNNNGTG 1 cut(s) 348
PaeR7I CTCGAG 1 cut(s) 332
PceI AGGCCT 1 cut(s) 203
PfeI GAWTC 1 cut(s) 640
PkrI GCNGC 1 cut(s) 369
PleI GAGTC 2 cut(s) 297, 429
PpsI GAGTC 2 cut(s) 297, 429
Psp6I CCWGG 1 cut(s) 203
PspFI CCCAGC 1 cut(s) 72
PspGI CCWGG 1 cut(s) 203
PspPI GGNCC 3 cut(s) 243, 499, 537
PstI CTGCAG 1 cut(s) 369
PstNI CAGNNNCTG 1 cut(s) 650
PsuI RGATCY 1 cut(s) 303
RsaI GTAC 3 cut(s) 234, 553, 631
RsaNI GTAC 3 cut(s) 233, 552, 630
RseI CAYNNNNRTG 2 cut(s) 141, 348
SatI GCNGC 1 cut(s) 368
Sau3AI GATC 1 cut(s) 303
Sau96I GGNCC 3 cut(s) 243, 499, 537
ScaI AGTACT 1 cut(s) 234
SchI GAGTC 2 cut(s) 298, 429
ScrFI CCNGG 1 cut(s) 205
SduI GDGCHC 3 cut(s) 138, 490, 518
SfcI CTRYAG 1 cut(s) 365
Sfr274I CTCGAG 1 cut(s) 332
SinI GGWCC 2 cut(s) 243, 537
SlaI CTCGAG 1 cut(s) 332
SmiMI CAYNNNNRTG 2 cut(s) 141, 348
SmlI CTYRAG 2 cut(s) 107, 332
SmoI CTYRAG 2 cut(s) 107, 332
Sse9I AATT 2 cut(s) 187, 411
SseBI AGGCCT 1 cut(s) 203
SspMI CTAG 2 cut(s) 483, 531
StuI AGGCCT 1 cut(s) 203
StyD4I CCNGG 1 cut(s) 203
TaiI ACGT 3 cut(s) 515, 591, 607
TaqI TCGA 3 cut(s) 12, 300, 333
TasI AATT 2 cut(s) 187, 411
TatI WGTACW 3 cut(s) 232, 551, 629
TfiI GAWTC 1 cut(s) 640
TscAI CASTG 2 cut(s) 495, 554
TseFI GTSAC 2 cut(s) 454, 584
TseI GCWGC 1 cut(s) 367
Tsp45I GTSAC 2 cut(s) 454, 584
TspDTI ATGAA 4 cut(s) 47, 159, 181, 195
TspRI CASTG 2 cut(s) 495, 554
VneI GTGCAC 2 cut(s) 134, 486
VpaK11BI GGWCC 2 cut(s) 243, 537
XagI CCTNNNNNAGG 1 cut(s) 449
XapI RAATTY 1 cut(s) 411
XhoI CTCGAG 1 cut(s) 332
XspI CTAG 2 cut(s) 483, 531
ZrmI AGTACT 1 cut(s) 234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.