FvH4_2g38770

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000864
Physical Location & Seq
Forward (+)
27988924 .. 27991720
2797 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g38770.t2

Sequence Viewer

Length: 1566 bp
ATGGAAAGGGAGAACAACAGTGGAAGTATTTCTAGCGAGGACTCTGGTTTTTTTGAAGAAGAAGCAAATGTGGACTCCGACGATGACTCAAACTATGTTGAAGGAGGTAACACAGACGCTAATGTGTTCTCTAGGGAGAATTGGTCAACTGTCTTCTTGAGAGAAGAAGAAGTTAGCCAACTTCAGGAGGATGATATTGCAGGAGTGTCTGCTCTCTTATCTGTAGCGAGATCTGCTGCATGCTTCCTACTATGTCACTTTAGTTGGAATGTCAATAAACTGCAGGAAGAATGGTTTTCTGATGAAGACAAAGTCAGAGAAACAGCTGGCTTGTTGAGGAAGCCGGTATTCCTGCATCAAGTAGGTAGTGTCAATTGTGGAATATGTTTTGAAGATTATGACTGTTCATATGATCAATCAGCCGATGGTGCTATTATCTCAGCTGCTTGTGGTCATCCGTTTTGTAAAGATTGTTGGACAGGTTATATTAGCACATCGATTAACGATGGGCCAGGATGTTTGATGTTGAGATGTCCTGAACCATCTTGTAGCGCAGCAGTTGATTGGGATTTGATCCGAAATGTGTTGAAAAACCAACATGATCATGAGATCGAGAGGTATAAGCGCTACCTTCTGAGATCTTATGTCGAAAAGCATAAGCAGATGAAATGGTGCCCTGCTCCGGACTGTCAGTATGCTATCAAAGTGTTTGACGAAGATTTGACTGGAAACGGAAACTGTTATGATGTTTCTTGCATTTGCTCTAATAGTTTCTGCTGGAATTGCGTTGAGGAGGTTCATAGTCCTGTCGGGTGCGACACTGTGGGAAAGTGGATTTTGAAGAATAAGGATGATTCTCAGAATGCACAATGGATTGTGGTTAATACTAAGCCATGTCCCAAGTGCAAAACACCGATCGAGAAGAACAATGGGTGTAATCATATGAGGTGCAGTCTGCCTTGTGGGCATCACTTTTGCTGGTTATGCCTCGGGCCACTTGGTTGCAAATGTAATAGTTTCCAACGAGCTGCAAACCAGAATGCGAAGAAGAAGGAGGAAAGTCAGAGTGAAAGGGAGAGGAAGAGGCTAAAGATGAGTTTAGAGAGATACAATCATTATTATGTTCGCTGGGCAAACAATCAAGCTTCAAAGAAAAAAGCTATTGAGTCATTGCATAAGGTGCAAACAGTGCACATTGTGAAACTTGCTAGCAGATCAGAACCCTCACTTACTCAGATTAATGATTTCGAGTTCATTATACAGGCATGGAAACAGATAATTGATTGTAGGCAGGTGATCAAATGGACCTATGCATATGGTTATTACATACCCGAGGAAAATAAATATGACAAGAAGCGGCAGCTGTTTGAGTACTTGCAGCGCCAAGCAGAGCACAGTCTGGAGAGGCTCCACAAATGTGCCGAGACTGAATTGAAAGAGACCCCGTTGGAGAAGTTCCCAGAGTTCCGTCTAAGGCTCATACAACTGACAAATGTGACCGGAACTTATTTTAAGAAGCTGGTTACAGCCCTGGAGAATGGCCTCTCGGAGGTGGAGACTAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IBR zf-RanBP

Protein Analysis

522

Amino Acids

59.92

Weight (kDa)

5.76

Isoelectric Point (pI)

50.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UBA_ARI1 PF21235 60 - 99 6.6e-10 E3 ubiquitin-protein ligase ARIH1, UBA-like domain
IBR PF01485 205 - 272 2.7e-15 IBR domain, a half RING-finger domain
IBR_2 PF22605 292 - 337 4.9e-11 IBR domain
IBR_1 PF22191 295 - 332 4.7e-10 IBR domain
Ariadne PF19422 368 - 515 2.6e-12 Ariadne domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1282
Acc36I ACCTGC 1 cut(s) 1282
AccB1I GGYRCC 1 cut(s) 672
AccIII TCCGGA 1 cut(s) 682
AciI CCGC 1 cut(s) 1357
AclWI GGATC 1 cut(s) 568
AcuI CTGAAG 1 cut(s) 167
AdeI CACNNNGTG 1 cut(s) 1198
AfaI GTAC 1 cut(s) 1373
AfeI AGCGCT 1 cut(s) 626
AfiI CCNNNNNNNGG 3 cut(s) 184, 682, 1549
AgsI TTSAA 7 cut(s) 56, 101, 392, 589, 841, 1149, 1435
AhlI ACTAGT 1 cut(s) 1559
AjnI CCWGG 2 cut(s) 511, 1530
AleI CACNNNNGTG 1 cut(s) 1416
AluBI AGCT 7 cut(s) 326, 443, 1028, 1145, 1160, 1363, 1519
AluI AGCT 7 cut(s) 326, 443, 1028, 1145, 1160, 1363, 1519
Alw21I GWGCWC 2 cut(s) 1194, 1395
Alw26I GTCTC 3 cut(s) 1418, 1433, 1550
Alw44I GTGCAC 1 cut(s) 1190
AlwI GGATC 1 cut(s) 568
Ama87I CYCGRG 2 cut(s) 989, 1331
Aor13HI TCCGGA 1 cut(s) 682
Aor51HI AGCGCT 1 cut(s) 626
AoxI GGCC 3 cut(s) 509, 992, 1540
ApaLI GTGCAC 1 cut(s) 1190
ApeKI GCWGC 6 cut(s) 236, 443, 554, 1028, 1360, 1378
AseI ATTAAT 1 cut(s) 1239
Asp700I GAANNNNTTC 1 cut(s) 28
AspLEI GCGC 3 cut(s) 554, 627, 1383
AspS9I GGNCC 3 cut(s) 509, 992, 1305
AsuHPI GGTGA 1 cut(s) 1306
AsuNHI GCTAGC 1 cut(s) 1208
AvaI CYCGRG 2 cut(s) 989, 1331
AvaII GGWCC 1 cut(s) 1305
BaeGI GKGCMC 2 cut(s) 677, 1194
BanI GGYRCC 1 cut(s) 672
BbsI GAAGAC 2 cut(s) 145, 312
Bbv12I GWGCWC 2 cut(s) 1194, 1395
BbvI GCAGC 6 cut(s) 223, 430, 566, 1015, 1372, 1390
BccI CCATC 3 cut(s) 419, 500, 550
BciT130I CCWGG 2 cut(s) 513, 1532
BclI TGATCA 3 cut(s) 412, 601, 1296
BcoDI GTCTC 3 cut(s) 1418, 1433, 1550
BcuI ACTAGT 1 cut(s) 1559
BfaI CTAG 4 cut(s) 33, 132, 1209, 1560
BfmI CTRYAG 2 cut(s) 222, 281
BfoI RGCGCY 2 cut(s) 628, 1384
BfuAI ACCTGC 1 cut(s) 1282
BglI GCCNNNNNGGC 1 cut(s) 964
BglII AGATCT 2 cut(s) 230, 638
BisI GCNGC 7 cut(s) 237, 444, 555, 1029, 1358, 1361, 1379
BlsI GCNGC 7 cut(s) 238, 445, 556, 1030, 1359, 1362, 1380
BmcAI AGTACT 1 cut(s) 1373
Bme1390I CCNGG 2 cut(s) 513, 1532
Bme18I GGWCC 1 cut(s) 1305
BmeT110I CYCGRG 2 cut(s) 989, 1331
BmgT120I GGNCC 3 cut(s) 509, 992, 1305
BmiI GGNNCC 2 cut(s) 674, 1409
BmrFI CCNGG 2 cut(s) 513, 1532
BmsI GCATC 2 cut(s) 364, 976
BmtI GCTAGC 1 cut(s) 1212
BpiI GAAGAC 2 cut(s) 145, 312
BpmI CTGGAG 2 cut(s) 1421, 1553
BpuEI CTTGAG 1 cut(s) 178
Bsa29I ATCGAT 1 cut(s) 497
BsaI GGTCTC 1 cut(s) 1433
BsaJI CCNNGG 3 cut(s) 988, 1332, 1530
BsaWI WCCGGW 2 cut(s) 682, 1499
BsaXI ACNNNNNCTCC 2 cut(s) 128, 158
Bsc4I CCNNNNNNNGG 3 cut(s) 184, 682, 1549
Bse118I RCCGGY 1 cut(s) 343
Bse1I ACTGG 1 cut(s) 730
Bse3DI GCAATG 1 cut(s) 1169
BseAI TCCGGA 1 cut(s) 682
BseBI CCWGG 2 cut(s) 513, 1532
BseCI ATCGAT 1 cut(s) 497
BseDI CCNNGG 3 cut(s) 988, 1332, 1530
BseGI GGATG 4 cut(s) 196, 454, 521, 856
BseLI CCNNNNNNNGG 3 cut(s) 184, 682, 1549
BseMI GCAATG 1 cut(s) 1169
BseMII CTCAG 4 cut(s) 453, 626, 872, 1247
BseNI ACTGG 1 cut(s) 730
BseRI GAGGAG 1 cut(s) 806
BseSI GKGCMC 2 cut(s) 677, 1194
BseXI GCAGC 6 cut(s) 223, 430, 566, 1015, 1372, 1390
BseYI CCCAGC 1 cut(s) 1128
BsgI GTGCAG 1 cut(s) 970
Bsh1285I CGRYCG 1 cut(s) 918
BshFI GGCC 3 cut(s) 511, 994, 1542
BshNI GGYRCC 1 cut(s) 672
BshVI ATCGAT 1 cut(s) 497
BsiEI CGRYCG 1 cut(s) 918
BsiHKAI GWGCWC 2 cut(s) 1194, 1395
BsiHKCI CYCGRG 2 cut(s) 989, 1331
BsiSI CCGG 3 cut(s) 344, 683, 1500
BslFI GGGAC 1 cut(s) 882
BslI CCNNNNNNNGG 3 cut(s) 184, 682, 1549
BsmAI GTCTC 3 cut(s) 1418, 1433, 1550
BsmFI GGGAC 1 cut(s) 882
BsmI GAATGC 2 cut(s) 868, 1045
BsnI GGCC 3 cut(s) 511, 994, 1542
Bso31I GGTCTC 1 cut(s) 1433
BsoBI CYCGRG 2 cut(s) 989, 1331
Bsp1286I GDGCHC 3 cut(s) 677, 1194, 1395
Bsp13I TCCGGA 1 cut(s) 682
Bsp143I GATC 9 cut(s) 230, 412, 573, 601, 609, 638, 915, 1214, 1296
BspACI CCGC 1 cut(s) 1357
BspANI GGCC 3 cut(s) 511, 994, 1542
BspCNI CTCAG 4 cut(s) 452, 627, 871, 1246
BspDI ATCGAT 1 cut(s) 497
BspEI TCCGGA 1 cut(s) 682
BspHI TCATGA 1 cut(s) 604
BspLI GGNNCC 2 cut(s) 674, 1409
BspMAI CTGCAG 1 cut(s) 285
BspMI ACCTGC 1 cut(s) 1282
BspOI GCTAGC 1 cut(s) 1212
BspPI GGATC 1 cut(s) 568
BspT107I GGYRCC 1 cut(s) 672
BspTNI GGTCTC 1 cut(s) 1433
BsrDI GCAATG 1 cut(s) 1169
BsrFI RCCGGY 1 cut(s) 343
BsrI ACTGG 1 cut(s) 730
BssAI RCCGGY 1 cut(s) 343
BssECI CCNNGG 3 cut(s) 988, 1332, 1530
BssMI GATC 9 cut(s) 230, 412, 573, 601, 609, 638, 915, 1214, 1296
Bst2UI CCWGG 2 cut(s) 513, 1532
Bst4CI ACNGT 8 cut(s) 20, 151, 404, 689, 740, 823, 1189, 1397
Bst6I CTCTTC 1 cut(s) 1076
BstAPI GCANNNNNTGC 2 cut(s) 1180, 1189
BstC8I GCNNGC 3 cut(s) 241, 328, 1210
BstDEI CTNAG 6 cut(s) 439, 635, 858, 888, 1233, 1472
BstENI CCTNNNNNAGG 1 cut(s) 1547
BstF5I GGATG 4 cut(s) 196, 454, 521, 856
BstH2I RGCGCY 2 cut(s) 628, 1384
BstHHI GCGC 3 cut(s) 554, 627, 1383
BstKTI GATC 9 cut(s) 233, 415, 576, 604, 612, 641, 918, 1217, 1299
BstMAI GTCTC 3 cut(s) 1418, 1433, 1550
BstMBI GATC 9 cut(s) 230, 412, 573, 601, 609, 638, 915, 1214, 1296
BstMCI CGRYCG 1 cut(s) 918
BstMWI GCNNNNNNNGC 7 cut(s) 233, 428, 783, 964, 984, 1180, 1189
BstNI CCWGG 2 cut(s) 513, 1532
BstNSI RCATGY 1 cut(s) 243
BstSCI CCNGG 2 cut(s) 511, 1530
BstSFI CTRYAG 2 cut(s) 222, 281
BstSLI GKGCMC 2 cut(s) 677, 1194
BstV1I GCAGC 6 cut(s) 223, 430, 566, 1015, 1372, 1390
BstV2I GAAGAC 2 cut(s) 145, 312
BstX2I RGATCY 2 cut(s) 230, 638
BstYI RGATCY 2 cut(s) 230, 638
Bsu15I ATCGAT 1 cut(s) 497
BsuRI GGCC 3 cut(s) 511, 994, 1542
BsuTUI ATCGAT 1 cut(s) 497
BtsCI GGATG 4 cut(s) 196, 454, 521, 856
BtsIMutI CAGTG 3 cut(s) 25, 819, 1194
BveI ACCTGC 1 cut(s) 1282
Cac8I GCNNGC 3 cut(s) 241, 328, 1210
CciI TCATGA 1 cut(s) 604
CfoI GCGC 3 cut(s) 554, 627, 1383
Cfr10I RCCGGY 1 cut(s) 343
Cfr13I GGNCC 3 cut(s) 509, 992, 1305
ClaI ATCGAT 1 cut(s) 497
CseI GACGC 1 cut(s) 125
Csp6I GTAC 1 cut(s) 1372
CviAII CATG 5 cut(s) 240, 599, 605, 894, 1266
CviQI GTAC 1 cut(s) 1372
DdeI CTNAG 6 cut(s) 439, 635, 858, 888, 1233, 1472
DpnI GATC 9 cut(s) 232, 414, 575, 603, 611, 640, 917, 1216, 1298
DpnII GATC 9 cut(s) 230, 412, 573, 601, 609, 638, 915, 1214, 1296
DraIII CACNNNGTG 1 cut(s) 1198
Eam1104I CTCTTC 1 cut(s) 1076
EarI CTCTTC 1 cut(s) 1076
Eco31I GGTCTC 1 cut(s) 1433
Eco47I GGWCC 1 cut(s) 1305
Eco47III AGCGCT 1 cut(s) 626
Eco57I CTGAAG 1 cut(s) 167
Eco88I CYCGRG 2 cut(s) 989, 1331
EcoNI CCTNNNNNAGG 1 cut(s) 1547
EcoRII CCWGG 2 cut(s) 511, 1530
EcoT22I ATGCAT 1 cut(s) 1315
FaeI CATG 5 cut(s) 243, 602, 608, 897, 1269
FaqI GGGAC 1 cut(s) 882
FatI CATG 5 cut(s) 239, 598, 604, 893, 1265
FauNDI CATATG 3 cut(s) 409, 942, 1315
FbaI TGATCA 3 cut(s) 412, 601, 1296
Fnu4HI GCNGC 7 cut(s) 237, 444, 555, 1029, 1358, 1361, 1379
FokI GGATG 4 cut(s) 203, 441, 528, 863
Fsp4HI GCNGC 7 cut(s) 237, 444, 555, 1029, 1358, 1361, 1379
FspBI CTAG 4 cut(s) 33, 132, 1209, 1560
GlaI GCGC 3 cut(s) 553, 626, 1382
GluI GCNGC 7 cut(s) 237, 444, 555, 1029, 1358, 1361, 1379
GsaI CCCAGC 1 cut(s) 1132
GsuI CTGGAG 2 cut(s) 1421, 1553
HaeII RGCGCY 2 cut(s) 628, 1384
HaeIII GGCC 3 cut(s) 511, 994, 1542
HapII CCGG 3 cut(s) 344, 683, 1500
HgaI GACGC 1 cut(s) 125
HhaI GCGC 3 cut(s) 554, 627, 1383
Hin1II CATG 5 cut(s) 243, 602, 608, 897, 1269
Hin6I GCGC 3 cut(s) 552, 625, 1381
HinP1I GCGC 3 cut(s) 552, 625, 1381
HincII GTYRAC 1 cut(s) 147
HindII GTYRAC 1 cut(s) 147
HindIII AAGCTT 1 cut(s) 1143
HinfI GANTC 5 cut(s) 41, 74, 86, 854, 1166
HpaII CCGG 3 cut(s) 344, 683, 1500
HphI GGTGA 1 cut(s) 1306
Hpy166II GTNNAC 3 cut(s) 73, 147, 1192
Hpy188III TCNNGA 8 cut(s) 157, 185, 536, 605, 613, 683, 919, 1400
Hpy8I GTNNAC 3 cut(s) 73, 147, 1192
Hpy99I CGWCG 1 cut(s) 83
HpyAV CCTTC 3 cut(s) 95, 641, 1045
HpyCH4III ACNGT 8 cut(s) 20, 151, 404, 689, 740, 823, 1189, 1397
HpyF10VI GCNNNNNNNGC 7 cut(s) 233, 428, 783, 964, 984, 1180, 1189
HpyF3I CTNAG 6 cut(s) 439, 635, 858, 888, 1233, 1472
Hsp92II CATG 5 cut(s) 243, 602, 608, 897, 1269
HspAI GCGC 3 cut(s) 552, 625, 1381
Kpn2I TCCGGA 1 cut(s) 682
Ksp22I TGATCA 3 cut(s) 412, 601, 1296
Kzo9I GATC 9 cut(s) 230, 412, 573, 601, 609, 638, 915, 1214, 1296
LmnI GCTCC 2 cut(s) 685, 1413
Lsp1109I GCAGC 6 cut(s) 223, 430, 566, 1015, 1372, 1390
LweI GCATC 2 cut(s) 364, 976
MaeI CTAG 4 cut(s) 33, 132, 1209, 1560
MaeIII GTNAC 4 cut(s) 107, 254, 1495, 1522
MalI GATC 9 cut(s) 232, 414, 575, 603, 611, 640, 917, 1216, 1298
MboI GATC 9 cut(s) 230, 412, 573, 601, 609, 638, 915, 1214, 1296
MfeI CAATTG 1 cut(s) 373
MflI RGATCY 2 cut(s) 230, 638
MhlI GDGCHC 3 cut(s) 677, 1194, 1395
MluCI AATT 5 cut(s) 139, 373, 781, 1278, 1430
MlyI GAGTC 4 cut(s) 35, 68, 80, 1175
MmeI TCCRAC 5 cut(s) 102, 245, 455, 1045, 1428
Mph1103I ATGCAT 1 cut(s) 1315
MroI TCCGGA 1 cut(s) 682
MroXI GAANNNNTTC 1 cut(s) 28
MseI TTAA 4 cut(s) 501, 882, 1239, 1512
MslI CAYNNNNRTG 3 cut(s) 603, 1119, 1416
MspA1I CMGCKG 3 cut(s) 326, 443, 1363
MspI CCGG 3 cut(s) 344, 683, 1500
MspR9I CCNGG 2 cut(s) 513, 1532
MunI CAATTG 1 cut(s) 373
Mva1269I GAATGC 2 cut(s) 868, 1045
MvaI CCWGG 2 cut(s) 513, 1532
MwoI GCNNNNNNNGC 7 cut(s) 233, 428, 783, 964, 984, 1180, 1189
NdeI CATATG 3 cut(s) 409, 942, 1315
NdeII GATC 9 cut(s) 230, 412, 573, 601, 609, 638, 915, 1214, 1296
NheI GCTAGC 1 cut(s) 1208
NlaIII CATG 5 cut(s) 243, 602, 608, 897, 1269
NlaIV GGNNCC 2 cut(s) 674, 1409
NmeAIII GCCGAG 1 cut(s) 1447
NmuCI GTSAC 2 cut(s) 254, 1495
NsiI ATGCAT 1 cut(s) 1315
NspI RCATGY 1 cut(s) 243
OliI CACNNNNGTG 1 cut(s) 1416
PaeI GCATGC 1 cut(s) 243
PagI TCATGA 1 cut(s) 604
PaqCI CACCTGC 1 cut(s) 1282
PctI GAATGC 2 cut(s) 868, 1045
PdmI GAANNNNTTC 1 cut(s) 28
PfeI GAWTC 1 cut(s) 854
PflFI GACNNNGTC 1 cut(s) 311
PkrI GCNGC 7 cut(s) 238, 445, 556, 1030, 1359, 1362, 1380
Ple19I CGATCG 1 cut(s) 918
PleI GAGTC 4 cut(s) 35, 68, 80, 1174
PpsI GAGTC 4 cut(s) 35, 68, 80, 1174
PshBI ATTAAT 1 cut(s) 1239
Psp6I CCWGG 2 cut(s) 511, 1530
PspFI CCCAGC 1 cut(s) 1128
PspGI CCWGG 2 cut(s) 511, 1530
PspN4I GGNNCC 2 cut(s) 674, 1409
PspPI GGNCC 3 cut(s) 509, 992, 1305
PstI CTGCAG 1 cut(s) 285
PsuI RGATCY 2 cut(s) 230, 638
PsyI GACNNNGTC 1 cut(s) 311
PvuI CGATCG 1 cut(s) 918
PvuII CAGCTG 3 cut(s) 326, 443, 1363
RsaI GTAC 1 cut(s) 1373
RsaNI GTAC 1 cut(s) 1372
RseI CAYNNNNRTG 3 cut(s) 603, 1119, 1416
SaqAI TTAA 4 cut(s) 501, 882, 1239, 1512
SatI GCNGC 7 cut(s) 237, 444, 555, 1029, 1358, 1361, 1379
Sau3AI GATC 9 cut(s) 230, 412, 573, 601, 609, 638, 915, 1214, 1296
Sau96I GGNCC 3 cut(s) 509, 992, 1305
ScaI AGTACT 1 cut(s) 1373
SchI GAGTC 4 cut(s) 35, 68, 80, 1175
ScrFI CCNGG 2 cut(s) 513, 1532
SduI GDGCHC 3 cut(s) 677, 1194, 1395
SfaNI GCATC 2 cut(s) 364, 976
SfcI CTRYAG 2 cut(s) 222, 281
SinI GGWCC 1 cut(s) 1305
SmiMI CAYNNNNRTG 3 cut(s) 603, 1119, 1416
SmlI CTYRAG 1 cut(s) 157
SmoI CTYRAG 1 cut(s) 157
SpeI ACTAGT 1 cut(s) 1559
SphI GCATGC 1 cut(s) 243
Sse9I AATT 5 cut(s) 139, 373, 781, 1278, 1430
SsiI CCGC 1 cut(s) 1357
SspMI CTAG 4 cut(s) 33, 132, 1209, 1560
StyD4I CCNGG 2 cut(s) 511, 1530
TaaI ACNGT 8 cut(s) 20, 151, 404, 689, 740, 823, 1189, 1397
TaqI TCGA 5 cut(s) 497, 612, 648, 918, 1248
TasI AATT 5 cut(s) 139, 373, 781, 1278, 1430
TatI WGTACW 1 cut(s) 1371
TauI GCSGC 1 cut(s) 1360
TfiI GAWTC 1 cut(s) 854
Tru1I TTAA 4 cut(s) 501, 882, 1239, 1512
Tru9I TTAA 4 cut(s) 501, 882, 1239, 1512
TscAI CASTG 3 cut(s) 25, 826, 1194
TseFI GTSAC 2 cut(s) 254, 1495
TseI GCWGC 6 cut(s) 236, 443, 554, 1028, 1360, 1378
Tsp45I GTSAC 2 cut(s) 254, 1495
TspDTI ATGAA 5 cut(s) 318, 396, 680, 788, 1243
TspGWI ACGGA 3 cut(s) 447, 747, 1457
TspRI CASTG 3 cut(s) 25, 826, 1194
Tth111I GACNNNGTC 1 cut(s) 311
VneI GTGCAC 1 cut(s) 1190
VpaK11BI GGWCC 1 cut(s) 1305
VspI ATTAAT 1 cut(s) 1239
XagI CCTNNNNNAGG 1 cut(s) 1547
XceI RCATGY 1 cut(s) 243
XmnI GAANNNNTTC 1 cut(s) 28
XspI CTAG 4 cut(s) 33, 132, 1209, 1560
ZrmI AGTACT 1 cut(s) 1373
Zsp2I ATGCAT 1 cut(s) 1315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.