Rorug06G0314800

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000864
Physical Location & Seq
Forward (+)
46618262 .. 46619216
955 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0314800.1

Sequence Viewer

Length: 411 bp
ATGGCCTCCTTCACAGCTAGCACTCCCACCTCATCTGTCACCAGGGCAGCCCTTGTGCACAAGCCATCTGTTGGGGCTCCATCCTCAACTATTCTAGCTTTGCCATCAATTGGAAACAAGGGAAAGGTGAGATGTTCAGTGGAGGCAAAGCCCCCAATGAACGGGAGCAACTCAAACATTGGGATGAGTGCATCTCTGGTAGCTGCAGCTTTGGCTGCAACCATGTCAAGCCCAGCAGCCATGGCTTTGGTGGATGACAGATTGAGCACAGAAGGAACAGGGCTTCCCTTTGGTTTGAGCAACAATCTTCTCGGGTGGATCCTTCTTGGTGTGTTTGGTCTCATCTGGGGACTCTACTTCACCTACACTTCAGATCTTGAAGAGGACGAGGAGTCAGGACTATCCCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IBR zf-RanBP

Protein Analysis

136

Amino Acids

13.91

Weight (kDa)

4.91

Isoelectric Point (pI)

46.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PsbW PF07123 1 - 136 5.3e-60 Photosystem II reaction centre W protein (PsbW)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 71, 110
AclWI GGATC 2 cut(s) 313, 326
AcuI CTGAAG 1 cut(s) 354
AfiI CCNNNNNNNGG 3 cut(s) 71, 110, 161
AgsI TTSAA 1 cut(s) 380
AhdI GACNNNNNGTC 1 cut(s) 391
AjnI CCWGG 1 cut(s) 41
AloI GAACNNNNNNTCC 2 cut(s) 268, 300
AluBI AGCT 4 cut(s) 17, 98, 203, 209
AluI AGCT 4 cut(s) 17, 98, 203, 209
Alw21I GWGCWC 2 cut(s) 60, 269
Alw26I GTCTC 1 cut(s) 344
Alw44I GTGCAC 1 cut(s) 56
AlwI GGATC 2 cut(s) 313, 326
Ama87I CYCGRG 1 cut(s) 311
AoxI GGCC 1 cut(s) 3
ApaLI GTGCAC 1 cut(s) 56
ApeKI GCWGC 5 cut(s) 47, 203, 206, 215, 236
AsuHPI GGTGA 3 cut(s) 31, 139, 352
AsuNHI GCTAGC 1 cut(s) 17
AvaI CYCGRG 1 cut(s) 311
BaeGI GKGCMC 1 cut(s) 60
BamHI GGATCC 1 cut(s) 318
BanII GRGCYC 1 cut(s) 79
Bbv12I GWGCWC 2 cut(s) 60, 269
BbvI GCAGC 5 cut(s) 59, 190, 202, 218, 248
BccI CCATC 3 cut(s) 73, 88, 112
BciT130I CCWGG 1 cut(s) 43
BcoDI GTCTC 1 cut(s) 344
BfaI CTAG 3 cut(s) 18, 95, 409
BfmI CTRYAG 1 cut(s) 204
BglII AGATCT 1 cut(s) 373
BisI GCNGC 5 cut(s) 48, 204, 207, 216, 237
BlsI GCNGC 5 cut(s) 49, 205, 208, 217, 238
Bme1390I CCNGG 1 cut(s) 43
BmeRI GACNNNNNGTC 1 cut(s) 391
BmeT110I CYCGRG 1 cut(s) 311
BmiI GGNNCC 2 cut(s) 78, 320
BmrFI CCNGG 1 cut(s) 43
BmsI GCATC 1 cut(s) 200
BmtI GCTAGC 1 cut(s) 21
BplI GAGNNNNNCTC 2 cut(s) 178, 210
BsaI GGTCTC 1 cut(s) 344
BsaJI CCNNGG 2 cut(s) 42, 240
Bsc4I CCNNNNNNNGG 3 cut(s) 71, 110, 161
BseBI CCWGG 1 cut(s) 43
BseDI CCNNGG 2 cut(s) 42, 240
BseGI GGATG 3 cut(s) 80, 189, 259
BseLI CCNNNNNNNGG 3 cut(s) 71, 110, 161
BseRI GAGGAG 1 cut(s) 404
BseSI GKGCMC 1 cut(s) 60
BseXI GCAGC 5 cut(s) 59, 190, 202, 218, 248
BseYI CCCAGC 1 cut(s) 232
BshFI GGCC 1 cut(s) 5
BsiHKAI GWGCWC 2 cut(s) 60, 269
BsiHKCI CYCGRG 1 cut(s) 311
BslFI GGGAC 1 cut(s) 363
BslI CCNNNNNNNGG 3 cut(s) 71, 110, 161
BsmAI GTCTC 1 cut(s) 344
BsmFI GGGAC 1 cut(s) 363
BsnI GGCC 1 cut(s) 5
Bso31I GGTCTC 1 cut(s) 344
BsoBI CYCGRG 1 cut(s) 311
Bsp1286I GDGCHC 3 cut(s) 60, 79, 269
Bsp143I GATC 2 cut(s) 318, 373
Bsp19I CCATGG 1 cut(s) 240
BspANI GGCC 1 cut(s) 5
BspLI GGNNCC 2 cut(s) 78, 320
BspMAI CTGCAG 1 cut(s) 208
BspOI GCTAGC 1 cut(s) 21
BspPI GGATC 2 cut(s) 313, 326
BspTNI GGTCTC 1 cut(s) 344
BssECI CCNNGG 2 cut(s) 42, 240
BssMI GATC 2 cut(s) 318, 373
BssT1I CCWWGG 1 cut(s) 240
Bst2UI CCWGG 1 cut(s) 43
Bst6I CTCTTC 1 cut(s) 375
BstC8I GCNNGC 1 cut(s) 19
BstDSI CCRYGG 1 cut(s) 240
BstF5I GGATG 3 cut(s) 80, 189, 259
BstKTI GATC 2 cut(s) 321, 376
BstMAI GTCTC 1 cut(s) 344
BstMBI GATC 2 cut(s) 318, 373
BstMWI GCNNNNNNNGC 3 cut(s) 212, 215, 242
BstNI CCWGG 1 cut(s) 43
BstSCI CCNGG 1 cut(s) 41
BstSFI CTRYAG 1 cut(s) 204
BstSLI GKGCMC 1 cut(s) 60
BstV1I GCAGC 5 cut(s) 59, 190, 202, 218, 248
BstX2I RGATCY 2 cut(s) 318, 373
BstXI CCANNNNNNTGG 1 cut(s) 247
BstYI RGATCY 2 cut(s) 318, 373
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 240
BtsCI GGATG 3 cut(s) 80, 189, 259
BtsIMutI CAGTG 1 cut(s) 144
Cac8I GCNNGC 1 cut(s) 19
CviAII CATG 2 cut(s) 223, 241
DpnI GATC 2 cut(s) 320, 375
DpnII GATC 2 cut(s) 318, 373
DriI GACNNNNNGTC 1 cut(s) 391
Eam1104I CTCTTC 1 cut(s) 375
Eam1105I GACNNNNNGTC 1 cut(s) 391
EarI CTCTTC 1 cut(s) 375
Eco130I CCWWGG 1 cut(s) 240
Eco24I GRGCYC 1 cut(s) 79
Eco31I GGTCTC 1 cut(s) 344
Eco57I CTGAAG 1 cut(s) 354
Eco88I CYCGRG 1 cut(s) 311
EcoRII CCWGG 1 cut(s) 41
EcoT14I CCWWGG 1 cut(s) 240
EcoT38I GRGCYC 1 cut(s) 79
ErhI CCWWGG 1 cut(s) 240
FaeI CATG 2 cut(s) 226, 244
FaiI YATR 2 cut(s) 224, 242
FaqI GGGAC 1 cut(s) 363
FatI CATG 2 cut(s) 222, 240
Fnu4HI GCNGC 5 cut(s) 48, 204, 207, 216, 237
FokI GGATG 3 cut(s) 67, 196, 266
FriOI GRGCYC 1 cut(s) 79
Fsp4HI GCNGC 5 cut(s) 48, 204, 207, 216, 237
FspBI CTAG 3 cut(s) 18, 95, 409
GluI GCNGC 5 cut(s) 48, 204, 207, 216, 237
GsaI CCCAGC 1 cut(s) 236
HaeIII GGCC 1 cut(s) 5
Hin1II CATG 2 cut(s) 226, 244
HinfI GANTC 2 cut(s) 351, 392
HphI GGTGA 3 cut(s) 31, 139, 352
Hpy166II GTNNAC 1 cut(s) 58
Hpy188I TCNGA 1 cut(s) 373
Hpy188III TCNNGA 2 cut(s) 377, 396
Hpy8I GTNNAC 1 cut(s) 58
HpyAV CCTTC 3 cut(s) 19, 266, 332
HpyCH4V TGCA 4 cut(s) 58, 191, 206, 218
HpyF10VI GCNNNNNNNGC 3 cut(s) 212, 215, 242
Hsp92II CATG 2 cut(s) 226, 244
Kzo9I GATC 2 cut(s) 318, 373
LmnI GCTCC 2 cut(s) 82, 165
LpnPI CCDG 7 cut(s) 28, 55, 182, 246, 264, 331, 381
Lsp1109I GCAGC 5 cut(s) 59, 190, 202, 218, 248
LweI GCATC 1 cut(s) 200
MaeI CTAG 3 cut(s) 18, 95, 409
MaeIII GTNAC 1 cut(s) 37
MalI GATC 2 cut(s) 320, 375
MboI GATC 2 cut(s) 318, 373
MboII GAAGA 2 cut(s) 299, 392
MfeI CAATTG 1 cut(s) 108
MflI RGATCY 2 cut(s) 318, 373
MhlI GDGCHC 3 cut(s) 60, 79, 269
MluCI AATT 1 cut(s) 108
MlyI GAGTC 2 cut(s) 345, 401
MnlI CCTC 6 cut(s) 16, 40, 94, 136, 376, 382
MslI CAYNNNNRTG 1 cut(s) 182
MspR9I CCNGG 1 cut(s) 43
MunI CAATTG 1 cut(s) 108
MvaI CCWGG 1 cut(s) 43
MwoI GCNNNNNNNGC 3 cut(s) 212, 215, 242
NcoI CCATGG 1 cut(s) 240
NdeII GATC 2 cut(s) 318, 373
NheI GCTAGC 1 cut(s) 17
NlaIII CATG 2 cut(s) 226, 244
NlaIV GGNNCC 2 cut(s) 78, 320
NmuCI GTSAC 1 cut(s) 37
PflMI CCANNNNNTGG 2 cut(s) 71, 110
PkrI GCNGC 5 cut(s) 49, 205, 208, 217, 238
PleI GAGTC 2 cut(s) 345, 400
PpsI GAGTC 2 cut(s) 345, 400
Psp6I CCWGG 1 cut(s) 41
PspFI CCCAGC 1 cut(s) 232
PspGI CCWGG 1 cut(s) 41
PspN4I GGNNCC 2 cut(s) 78, 320
PstI CTGCAG 1 cut(s) 208
PsuI RGATCY 2 cut(s) 318, 373
RseI CAYNNNNRTG 1 cut(s) 182
SatI GCNGC 5 cut(s) 48, 204, 207, 216, 237
Sau3AI GATC 2 cut(s) 318, 373
SchI GAGTC 2 cut(s) 345, 401
ScrFI CCNGG 1 cut(s) 43
SduI GDGCHC 3 cut(s) 60, 79, 269
SetI ASST 7 cut(s) 19, 32, 100, 129, 205, 211, 365
SfaNI GCATC 1 cut(s) 200
SfcI CTRYAG 1 cut(s) 204
SmiMI CAYNNNNRTG 1 cut(s) 182
Sse9I AATT 1 cut(s) 108
SspMI CTAG 3 cut(s) 18, 95, 409
StyD4I CCNGG 1 cut(s) 41
StyI CCWWGG 1 cut(s) 240
TasI AATT 1 cut(s) 108
TscAI CASTG 1 cut(s) 144
TseFI GTSAC 1 cut(s) 37
TseI GCWGC 5 cut(s) 47, 203, 206, 215, 236
Tsp45I GTSAC 1 cut(s) 37
TspDTI ATGAA 1 cut(s) 173
TspRI CASTG 1 cut(s) 144
Van91I CCANNNNNTGG 2 cut(s) 71, 110
VneI GTGCAC 1 cut(s) 56
XcmI CCANNNNNNNNNTGG 1 cut(s) 247
XspI CTAG 3 cut(s) 18, 95, 409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.