RLG00000024363

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000864
Physical Location & Seq
Reverse (-)
35145380 .. 35151685
6306 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024363

Sequence Viewer

Length: 1263 bp
ATGCACTACGAGAGCGAAGAAGACTACACAACCGTATATAGTGGTGATGAAGATGCAGGGATCTACAGCGCATCCGATGACGATTTTGATCTTCCAGAAGATGACAAAGTCAATGTTTCTGGTGAGATTAATTATACTTTTCTGAAAGAAGAAGAAATTGCCAAGCTTCAGAAAGATTATATTGCAGAAGTGTCTACGGTCCTTTCCATATCAAAATCCGATGCATGCTTACTGCTCCTTTACTTTAATTGGAGGGACGAGGAACTAAAGGATGAATGGTTTGCTGATGAAGAAAAAGTTCGTGAGAATGCAGGCTTGTTGGAGAAACCAATGGTTAAACATAGTGCCAGTGCAAATCAACCAATTACTTGCGGGATATGTTTTGATGAGTATTACTATTCAGCAGAGAGTACTATCAGTAGTATTTTGTCATCCTCTTGTGGTCATCCTTATTGCCGAGATTGCTGGGAAGGTTATATTAGCACATCAATCTGTGACTCCGGTCCGGCATGTTTGATGCTGACATGTCCTGAACCATCTTGTAAGGCAGCAGTTGGCCCTGAATTAATCGAAAACGTGCTGGTGGTGACAGGAAAAAAAGCCGAGTATGACAAGTACAAGCATTACCTGTTAAGATCATATGTCGAACATAACAGGAAGAAGATTAGATGGTGTCCTGCTCCGGATTGCAAGAGGGCAACTGATATGTCAGGGGAAGTTTGGGGCGCCGCTGAGAAAGAAAGAATGCAGGCAAAGATAGATTTAGAGAGATATGCTCATTACTATGAACGTTGGGCAAACAATGGATCATCAAAGGAAAGAGCTATCAAGGATTTTGATAAGGTGAAGACCGTGCACATTAAGAAACTTGGACACATAAAGAAAAATACAATAGAGCATAAGTACGATTTTATCATAGAGGCCTGGCAACAGATAATTGAATGCAGGCAGGTATTGCGATGGACCTATGCTTATGGATACTACATGAAGGAGAATGAGGATGCCAAGCGTCATCTCTTCGAGCACTTGCAAGGCCATGCCGAGTTCAGCCTGGAGAGGCTTCACAAATGTGCAGAGAGTGAACTGCAACAGTTTCTCAACCTGGCTCATGACGATAAGGTTTGGTTCATAGAGTTCCAAAAGAAACTCATTAGTCTGACAAAGGTGACTGGTACTTATTTCGACAGGCTGGTCACTGCGTTGGAGGACGGCCTGTCTGAGATTTTAACGAAGAAACAGAAAAAGAAAGTTCTTCGAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IBR zf-RanBP

Protein Analysis

421

Amino Acids

48.81

Weight (kDa)

5.51

Isoelectric Point (pI)

54.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UBA_ARI1 PF21235 56 - 94 7.1e-09 E3 ubiquitin-protein ligase ARIH1, UBA-like domain
Ariadne PF19422 256 - 407 1.8e-08 Ariadne domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1190
Acc36I ACCTGC 1 cut(s) 940
AccB1I GGYRCC 1 cut(s) 725
AccI GTMKAC 1 cut(s) 194
AccIII TCCGGA 1 cut(s) 682
AciI CCGC 2 cut(s) 372, 729
AclI AACGTT 1 cut(s) 790
AclWI GGATC 2 cut(s) 68, 814
AcuI CTGAAG 1 cut(s) 152
AcyI GRCGYC 1 cut(s) 726
AfaI GTAC 4 cut(s) 412, 617, 905, 1174
AflIII ACRYGT 1 cut(s) 524
AgsI TTSAA 1 cut(s) 941
AhdI GACNNNNNGTC 1 cut(s) 1213
AjnI CCWGG 3 cut(s) 923, 1050, 1101
AleI CACNNNNGTG 1 cut(s) 1068
AluBI AGCT 2 cut(s) 166, 824
AluI AGCT 2 cut(s) 166, 824
Alw21I GWGCWC 2 cut(s) 858, 1026
Alw44I GTGCAC 1 cut(s) 854
AlwI GGATC 2 cut(s) 68, 814
Aor13HI TCCGGA 1 cut(s) 682
AoxI GGCC 4 cut(s) 556, 921, 1033, 1210
ApaLI GTGCAC 1 cut(s) 854
ApeKI GCWGC 1 cut(s) 548
AseI ATTAAT 2 cut(s) 129, 566
Asp700I GAANNNNTTC 1 cut(s) 297
AspLEI GCGC 2 cut(s) 71, 728
AspS9I GGNCC 4 cut(s) 199, 503, 557, 963
AsuHPI GGTGA 5 cut(s) 56, 134, 598, 856, 1177
AsuII TTCGAA 1 cut(s) 1255
AvaII GGWCC 3 cut(s) 199, 503, 963
BaeGI GKGCMC 1 cut(s) 858
BanI GGYRCC 1 cut(s) 725
BbsI GAAGAC 2 cut(s) 27, 854
Bbv12I GWGCWC 2 cut(s) 858, 1026
BbvI GCAGC 1 cut(s) 560
BccI CCATC 3 cut(s) 544, 663, 954
BceAI ACGGC 1 cut(s) 1225
BciT130I CCWGG 3 cut(s) 925, 1052, 1103
BciVI GTATCC 1 cut(s) 971
BfmI CTRYAG 1 cut(s) 64
BfoI RGCGCY 1 cut(s) 729
BfuAI ACCTGC 1 cut(s) 940
BfuI GTATCC 1 cut(s) 971
BisI GCNGC 2 cut(s) 549, 729
BlsI GCNGC 2 cut(s) 550, 730
BmcAI AGTACT 1 cut(s) 412
Bme1390I CCNGG 3 cut(s) 925, 1052, 1103
Bme18I GGWCC 3 cut(s) 199, 503, 963
BmeRI GACNNNNNGTC 1 cut(s) 1213
BmgT120I GGNCC 4 cut(s) 199, 503, 557, 963
BmiI GGNNCC 1 cut(s) 727
BmrFI CCNGG 3 cut(s) 925, 1052, 1103
BmsI GCATC 5 cut(s) 43, 80, 211, 507, 991
BoxI GACNNNNGTC 1 cut(s) 501
BpiI GAAGAC 2 cut(s) 27, 854
BplI GAGNNNNNCTC 2 cut(s) 760, 792
BpmI CTGGAG 1 cut(s) 1073
Bpu14I TTCGAA 1 cut(s) 1255
BsaBI GATNNNNATC 1 cut(s) 87
BsaHI GRCGYC 1 cut(s) 726
BsaWI WCCGGW 2 cut(s) 500, 682
Bse1I ACTGG 2 cut(s) 348, 1174
Bse8I GATNNNNATC 1 cut(s) 87
BseAI TCCGGA 1 cut(s) 682
BseBI CCWGG 3 cut(s) 925, 1052, 1103
BseGI GGATG 5 cut(s) 71, 277, 431, 445, 1006
BseJI GATNNNNATC 1 cut(s) 87
BseMII CTCAG 2 cut(s) 723, 1209
BseNI ACTGG 2 cut(s) 348, 1174
BseSI GKGCMC 1 cut(s) 858
BseXI GCAGC 1 cut(s) 560
BseYI CCCAGC 1 cut(s) 465
BsgI GTGCAG 1 cut(s) 1092
BshFI GGCC 4 cut(s) 558, 923, 1035, 1212
BshNI GGYRCC 1 cut(s) 725
BsiHKAI GWGCWC 2 cut(s) 858, 1026
BsiSI CCGG 3 cut(s) 501, 506, 683
BslFI GGGAC 1 cut(s) 269
BsmFI GGGAC 1 cut(s) 269
BsmI GAATGC 3 cut(s) 313, 750, 947
BsnI GGCC 4 cut(s) 558, 923, 1035, 1212
Bsp119I TTCGAA 1 cut(s) 1255
Bsp1286I GDGCHC 2 cut(s) 858, 1026
Bsp13I TCCGGA 1 cut(s) 682
Bsp143I GATC 4 cut(s) 60, 88, 635, 806
BspACI CCGC 2 cut(s) 372, 729
BspANI GGCC 4 cut(s) 558, 923, 1035, 1212
BspCNI CTCAG 2 cut(s) 724, 1210
BspEI TCCGGA 1 cut(s) 682
BspHI TCATGA 1 cut(s) 1108
BspLI GGNNCC 1 cut(s) 727
BspMI ACCTGC 1 cut(s) 940
BspPI GGATC 2 cut(s) 68, 814
BspT104I TTCGAA 1 cut(s) 1255
BspT107I GGYRCC 1 cut(s) 725
BsrI ACTGG 2 cut(s) 348, 1174
BssMI GATC 4 cut(s) 60, 88, 635, 806
BssNI GRCGYC 1 cut(s) 726
Bst2UI CCWGG 3 cut(s) 925, 1052, 1103
Bst4CI ACNGT 4 cut(s) 34, 199, 853, 1092
Bst6I CTCTTC 1 cut(s) 1022
BstACI GRCGYC 1 cut(s) 726
BstAPI GCANNNNNTGC 1 cut(s) 955
BstBI TTCGAA 1 cut(s) 1255
BstC8I GCNNGC 4 cut(s) 226, 313, 750, 947
BstDEI CTNAG 2 cut(s) 732, 1218
BstF5I GGATG 5 cut(s) 71, 277, 431, 445, 1006
BstH2I RGCGCY 1 cut(s) 729
BstHHI GCGC 2 cut(s) 71, 728
BstKTI GATC 4 cut(s) 63, 91, 638, 809
BstMBI GATC 4 cut(s) 60, 88, 635, 806
BstMWI GCNNNNNNNGC 2 cut(s) 462, 955
BstNI CCWGG 3 cut(s) 925, 1052, 1103
BstNSI RCATGY 3 cut(s) 228, 513, 528
BstPAI GACNNNNGTC 1 cut(s) 501
BstSCI CCNGG 3 cut(s) 923, 1050, 1101
BstSFI CTRYAG 1 cut(s) 64
BstSLI GKGCMC 1 cut(s) 858
BstV1I GCAGC 1 cut(s) 560
BstV2I GAAGAC 2 cut(s) 27, 854
BstX2I RGATCY 1 cut(s) 60
BstYI RGATCY 1 cut(s) 60
BsuI GTATCC 1 cut(s) 971
BsuRI GGCC 4 cut(s) 558, 923, 1035, 1212
BtgZI GCGATG 1 cut(s) 973
BtsCI GGATG 5 cut(s) 71, 277, 431, 445, 1006
BtsI GCAGTG 1 cut(s) 1194
BtsIMutI CAGTG 2 cut(s) 355, 1194
BveI ACCTGC 1 cut(s) 940
Cac8I GCNNGC 4 cut(s) 226, 313, 750, 947
CciI TCATGA 1 cut(s) 1108
CfoI GCGC 2 cut(s) 71, 728
Cfr13I GGNCC 4 cut(s) 199, 503, 557, 963
CpoI CGGWCCG 1 cut(s) 503
CseI GACGC 1 cut(s) 998
Csp6I GTAC 4 cut(s) 411, 616, 904, 1173
CspI CGGWCCG 1 cut(s) 503
CviAII CATG 6 cut(s) 225, 510, 525, 985, 1037, 1109
CviQI GTAC 4 cut(s) 411, 616, 904, 1173
DdeI CTNAG 2 cut(s) 732, 1218
DinI GGCGCC 1 cut(s) 727
DpnI GATC 4 cut(s) 62, 90, 637, 808
DpnII GATC 4 cut(s) 60, 88, 635, 806
DrdI GACNNNNNNGTC 1 cut(s) 1190
DriI GACNNNNNGTC 1 cut(s) 1213
DseDI GACNNNNNNGTC 1 cut(s) 1190
Eam1104I CTCTTC 1 cut(s) 1022
Eam1105I GACNNNNNGTC 1 cut(s) 1213
EarI CTCTTC 1 cut(s) 1022
Eco147I AGGCCT 1 cut(s) 923
Eco47I GGWCC 3 cut(s) 199, 503, 963
Eco57I CTGAAG 1 cut(s) 152
EcoRII CCWGG 3 cut(s) 923, 1050, 1101
EcoT22I ATGCAT 1 cut(s) 226
EgeI GGCGCC 1 cut(s) 727
EheI GGCGCC 1 cut(s) 727
FaeI CATG 6 cut(s) 228, 513, 528, 988, 1040, 1112
FaqI GGGAC 1 cut(s) 269
FatI CATG 6 cut(s) 224, 509, 524, 984, 1036, 1108
FauI CCCGC 1 cut(s) 365
FauNDI CATATG 1 cut(s) 640
FblI GTMKAC 1 cut(s) 194
Fnu4HI GCNGC 2 cut(s) 549, 729
FokI GGATG 5 cut(s) 58, 284, 418, 432, 1013
Fsp4HI GCNGC 2 cut(s) 549, 729
GlaI GCGC 2 cut(s) 70, 727
GluI GCNGC 2 cut(s) 549, 729
GsaI CCCAGC 1 cut(s) 469
GsuI CTGGAG 1 cut(s) 1073
HaeII RGCGCY 1 cut(s) 729
HaeIII GGCC 4 cut(s) 558, 923, 1035, 1212
HapII CCGG 3 cut(s) 501, 506, 683
HgaI GACGC 1 cut(s) 998
HhaI GCGC 2 cut(s) 71, 728
Hin1I GRCGYC 1 cut(s) 726
Hin1II CATG 6 cut(s) 228, 513, 528, 988, 1040, 1112
Hin6I GCGC 2 cut(s) 69, 726
HinP1I GCGC 2 cut(s) 69, 726
HindIII AAGCTT 1 cut(s) 164
HinfI GANTC 1 cut(s) 497
HpaII CCGG 3 cut(s) 501, 506, 683
HphI GGTGA 5 cut(s) 56, 134, 598, 856, 1177
Hpy166II GTNNAC 3 cut(s) 195, 856, 1082
Hpy188I TCNGA 6 cut(s) 76, 144, 171, 220, 1158, 1219
Hpy188III TCNNGA 5 cut(s) 95, 302, 530, 683, 1109
Hpy8I GTNNAC 3 cut(s) 195, 856, 1082
HpyAV CCTTC 2 cut(s) 464, 982
HpyCH4III ACNGT 4 cut(s) 34, 199, 853, 1092
HpyCH4IV ACGT 2 cut(s) 576, 790
HpyF10VI GCNNNNNNNGC 2 cut(s) 462, 955
HpyF3I CTNAG 2 cut(s) 732, 1218
HpySE526I ACGT 2 cut(s) 576, 790
Hsp92I GRCGYC 1 cut(s) 726
Hsp92II CATG 6 cut(s) 228, 513, 528, 988, 1040, 1112
HspAI GCGC 2 cut(s) 69, 726
KasI GGCGCC 1 cut(s) 725
Kpn2I TCCGGA 1 cut(s) 682
Kzo9I GATC 4 cut(s) 60, 88, 635, 806
LmnI GCTCC 2 cut(s) 240, 685
Lsp1109I GCAGC 1 cut(s) 560
LweI GCATC 5 cut(s) 43, 80, 211, 507, 991
MaeII ACGT 2 cut(s) 576, 790
MaeIII GTNAC 4 cut(s) 494, 586, 1165, 1192
MalI GATC 4 cut(s) 62, 90, 637, 808
MboI GATC 4 cut(s) 60, 88, 635, 806
MflI RGATCY 1 cut(s) 60
MhlI GDGCHC 2 cut(s) 858, 1026
MluCI AATT 6 cut(s) 130, 156, 247, 363, 563, 936
Mly113I GGCGCC 1 cut(s) 726
MlyI GAGTC 1 cut(s) 491
MmeI TCCRAC 2 cut(s) 300, 1182
MnlI CCTC 8 cut(s) 246, 253, 445, 687, 913, 991, 1050, 1198
Mph1103I ATGCAT 1 cut(s) 226
MroI TCCGGA 1 cut(s) 682
MroXI GAANNNNTTC 1 cut(s) 297
MseI TTAA 7 cut(s) 129, 246, 336, 566, 632, 861, 1226
MslI CAYNNNNRTG 2 cut(s) 783, 1068
MspA1I CMGCKG 1 cut(s) 731
MspI CCGG 3 cut(s) 501, 506, 683
MspR9I CCNGG 3 cut(s) 925, 1052, 1103
Mva1269I GAATGC 3 cut(s) 313, 750, 947
MvaI CCWGG 3 cut(s) 925, 1052, 1103
MwoI GCNNNNNNNGC 2 cut(s) 462, 955
NarI GGCGCC 1 cut(s) 726
NdeI CATATG 1 cut(s) 640
NdeII GATC 4 cut(s) 60, 88, 635, 806
NlaIII CATG 6 cut(s) 228, 513, 528, 988, 1040, 1112
NlaIV GGNNCC 1 cut(s) 727
NmeAIII GCCGAG 3 cut(s) 482, 628, 1066
NmuCI GTSAC 4 cut(s) 494, 586, 1165, 1192
NsiI ATGCAT 1 cut(s) 226
NspI RCATGY 3 cut(s) 228, 513, 528
NspV TTCGAA 1 cut(s) 1255
OliI CACNNNNGTG 1 cut(s) 1068
PaeI GCATGC 1 cut(s) 228
PagI TCATGA 1 cut(s) 1108
PceI AGGCCT 1 cut(s) 923
PciI ACATGT 1 cut(s) 524
PctI GAATGC 3 cut(s) 313, 750, 947
PdmI GAANNNNTTC 1 cut(s) 297
PflFI GACNNNGTC 1 cut(s) 107
PkrI GCNGC 2 cut(s) 550, 730
PleI GAGTC 1 cut(s) 491
PluTI GGCGCC 1 cut(s) 729
PpsI GAGTC 1 cut(s) 491
PscI ACATGT 1 cut(s) 524
PshAI GACNNNNGTC 1 cut(s) 501
PshBI ATTAAT 2 cut(s) 129, 566
Psp1406I AACGTT 1 cut(s) 790
Psp6I CCWGG 3 cut(s) 923, 1050, 1101
PspFI CCCAGC 1 cut(s) 465
PspGI CCWGG 3 cut(s) 923, 1050, 1101
PspN4I GGNNCC 1 cut(s) 727
PspPI GGNCC 4 cut(s) 199, 503, 557, 963
PsuI RGATCY 1 cut(s) 60
PsyI GACNNNGTC 1 cut(s) 107
RsaI GTAC 4 cut(s) 412, 617, 905, 1174
RsaNI GTAC 4 cut(s) 411, 616, 904, 1173
RseI CAYNNNNRTG 2 cut(s) 783, 1068
Rsr2I CGGWCCG 1 cut(s) 503
RsrII CGGWCCG 1 cut(s) 503
SaqAI TTAA 7 cut(s) 129, 246, 336, 566, 632, 861, 1226
SatI GCNGC 2 cut(s) 549, 729
Sau3AI GATC 4 cut(s) 60, 88, 635, 806
Sau96I GGNCC 4 cut(s) 199, 503, 557, 963
ScaI AGTACT 1 cut(s) 412
SchI GAGTC 1 cut(s) 491
ScrFI CCNGG 3 cut(s) 925, 1052, 1103
SduI GDGCHC 2 cut(s) 858, 1026
SfaNI GCATC 5 cut(s) 43, 80, 211, 507, 991
SfcI CTRYAG 1 cut(s) 64
SfoI GGCGCC 1 cut(s) 727
SfuI TTCGAA 1 cut(s) 1255
SinI GGWCC 3 cut(s) 199, 503, 963
SmiMI CAYNNNNRTG 2 cut(s) 783, 1068
SphI GCATGC 1 cut(s) 228
Sse9I AATT 6 cut(s) 130, 156, 247, 363, 563, 936
SseBI AGGCCT 1 cut(s) 923
SsiI CCGC 2 cut(s) 372, 729
SspDI GGCGCC 1 cut(s) 725
StuI AGGCCT 1 cut(s) 923
StyD4I CCNGG 3 cut(s) 923, 1050, 1101
TaaI ACNGT 4 cut(s) 34, 199, 853, 1092
TaiI ACGT 2 cut(s) 579, 793
TaqI TCGA 5 cut(s) 570, 645, 1020, 1182, 1255
TasI AATT 6 cut(s) 130, 156, 247, 363, 563, 936
TatI WGTACW 2 cut(s) 410, 615
TauI GCSGC 1 cut(s) 731
Tru1I TTAA 7 cut(s) 129, 246, 336, 566, 632, 861, 1226
Tru9I TTAA 7 cut(s) 129, 246, 336, 566, 632, 861, 1226
TscAI CASTG 2 cut(s) 355, 1201
TseFI GTSAC 4 cut(s) 494, 586, 1165, 1192
TseI GCWGC 1 cut(s) 548
Tsp45I GTSAC 4 cut(s) 494, 586, 1165, 1192
TspDTI ATGAA 6 cut(s) 63, 288, 303, 801, 1001, 1117
TspRI CASTG 2 cut(s) 355, 1201
Tth111I GACNNNGTC 1 cut(s) 107
VneI GTGCAC 1 cut(s) 854
VpaK11BI GGWCC 3 cut(s) 199, 503, 963
VspI ATTAAT 2 cut(s) 129, 566
XceI RCATGY 3 cut(s) 228, 513, 528
XmiI GTMKAC 1 cut(s) 194
XmnI GAANNNNTTC 1 cut(s) 297
ZrmI AGTACT 1 cut(s) 412
Zsp2I ATGCAT 1 cut(s) 226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.