Rmu_sc0001670.1_g000028

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001670.1
OG0000864
Physical Location & Seq
Forward (+)
135355 .. 136848
1494 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001670.1_g000028.1.cds

Sequence Viewer

Length: 1494 bp
atggatgacacagactacatgtacgacgacgacggctttagcgattgtgacgacgacgacgatgatgatcagtcgattgaagaggaatcaaatgatcggcaaaactacactgttctgaaagaagaagagattgccaagcttcaagaggatgctatcactgaagtgtctgctgtactatcagtatcgcaatccgctgcctgcttcgtcctcactcagttcaactggaacgtgatcaatctcaacgacgagtggttcgccgatgaagataggatccggcaaaaggtaggtctgttggagcagcctgtggttcaattcaatccgaaaaaccaaagaactatttgcggtatatgttttgaggtgtatggctatggatcgatgtattcggccgcctgcggtcatcgttattgtggagagtgctggaggggttacatcagcacagcgatcaaggatggccccgcgtgtttgatgctgaggtgtcccgaaccgccgtgtagggcggcggttggtcctgatttgatctcggtcttggtgtcgaaacaggagtacgagaggtacaggcactaccttttgagatcgtacgtcgaacagcagaccaagatcaagtggtgtcctgctccggattgtaagtacgctgttaggtttgatagttatgagaccagaagctatgatgtttcgtgtctttgttcgcatagctattgctggaattgtacggaggagagtcaccgtccggtggactgtgagacggtgaggaagtgggttatgaagaacaaggacagctcgcaaaatgcgcagtggatattggttaacaccaagccttgtccaaagtgcaagagaccgattcagaagaaccaagggtgtcaccatatgacttgctcagctccttgtaggtatgagttttgctggtattgtcttgcgaaattcgaaagttattctactagatgttgtaacgcgtatagggccaatactgatgagctcaaggagttggatttggagccggatcagaagaagctgaaggagtatttagataggtatcttcattattatgagcgatgggcaaacaatcagtcttcgaaggaaaaggctgtcgagtctttagaggaggtgcgaaccaagcacattatggagcttattaggaaacaacagtatctgtctgagcttgaactcaatttcatcatagatgcatggcaacagataattgaatgtaggcaggtgctgcggtggagctatgcgtatggctactacatgcctgaggatgagcgtcttaaggctgatctgtttgagcacttgcaaggtcatgcagagtacagcctcgagaggcttcacaaatgcgccgagactgaattgcaaccatttctggcttctgaggtagaattaaaggagagcttccacgcatttcgcttaaaactcgaaaatatgacgagggccactaaagattatttcaggaagctggttacagccttggagaatggcctttcggaggtggacggcgtatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IBR zf-RanBP

Protein Analysis

497

Amino Acids

58.45

Weight (kDa)

5.08

Isoelectric Point (pI)

54.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1198
Acc16I TGCGCA 1 cut(s) 789
Acc36I ACCTGC 1 cut(s) 1198
AccII CGCG 2 cut(s) 458, 950
AccIII TCCGGA 1 cut(s) 616
AciI CCGC 9 cut(s) 192, 342, 387, 393, 456, 485, 497, 500, 1216
AclWI GGATC 4 cut(s) 265, 278, 379, 1005
AcoI YGGCCR 1 cut(s) 384
AcsI RAATTY 1 cut(s) 917
AcuI CTGAAG 2 cut(s) 180, 1031
AfaI GTAC 8 cut(s) 23, 174, 545, 554, 578, 629, 709, 1304
AfiI CCNNNNNNNGG 3 cut(s) 280, 730, 1477
AflII CTTAAG 1 cut(s) 1262
AflIII ACRYGT 2 cut(s) 18, 948
AgsI TTSAA 7 cut(s) 80, 143, 220, 311, 316, 1160, 1199
AleI CACNNNNGTG 1 cut(s) 161
Alw21I GWGCWC 2 cut(s) 975, 1284
Alw26I GTCTC 4 cut(s) 647, 734, 826, 1328
AlwI GGATC 4 cut(s) 265, 278, 379, 1005
AlwNI CAGNNNCTG 2 cut(s) 1147, 1213
Ama87I CYCGRG 1 cut(s) 1310
Aor13HI TCCGGA 1 cut(s) 616
AoxI GGCC 5 cut(s) 384, 451, 957, 1422, 1468
ApeKI GCWGC 3 cut(s) 194, 298, 1213
ApoI RAATTY 1 cut(s) 917
ArsI GACNNNNNNTTYG 6 cut(s) 236, 268, 271, 303, 1049, 1081
AspLEI GCGC 2 cut(s) 790, 1331
AspS9I GGNCC 4 cut(s) 452, 506, 957, 1422
AsuHPI GGTGA 3 cut(s) 713, 757, 851
AsuII TTCGAA 2 cut(s) 921, 1070
AvaI CYCGRG 1 cut(s) 1310
AvaII GGWCC 1 cut(s) 506
AxyI CCTNAGG 1 cut(s) 1248
BaeI ACNNNNGTAYC 2 cut(s) 1127, 1160
BamHI GGATCC 1 cut(s) 270
BanII GRGCYC 1 cut(s) 975
BbsI GAAGAC 1 cut(s) 1059
Bbv12I GWGCWC 2 cut(s) 975, 1284
BbvCI CCTCAGC 1 cut(s) 470
BbvI GCAGC 3 cut(s) 181, 310, 1200
BccI CCATC 2 cut(s) 443, 1044
BceAI ACGGC 2 cut(s) 49, 472
BclI TGATCA 2 cut(s) 67, 231
BcoDI GTCTC 4 cut(s) 647, 734, 826, 1328
BfaI CTAG 1 cut(s) 936
BfrI CTTAAG 1 cut(s) 1262
BfuAI ACCTGC 1 cut(s) 1198
BisI GCNGC 5 cut(s) 195, 299, 387, 498, 1214
BlpI GCTNAGC 1 cut(s) 874
BlsI GCNGC 5 cut(s) 196, 300, 388, 499, 1215
Bme18I GGWCC 1 cut(s) 506
BmeT110I CYCGRG 1 cut(s) 1310
BmgT120I GGNCC 4 cut(s) 452, 506, 957, 1422
BmiI GGNNCC 3 cut(s) 272, 454, 993
BmsI GCATC 3 cut(s) 139, 456, 1168
BpiI GAAGAC 1 cut(s) 1059
BpmI CTGGAG 1 cut(s) 439
Bpu10I CCTNAGC 1 cut(s) 470
Bpu1102I GCTNAGC 1 cut(s) 874
Bpu14I TTCGAA 2 cut(s) 921, 1070
BpuEI CTTGAG 1 cut(s) 959
Bsa29I ATCGAT 1 cut(s) 374
BsaBI GATNNNNATC 2 cut(s) 66, 1029
BsaI GGTCTC 2 cut(s) 647, 826
BsaJI CCNNGG 2 cut(s) 850, 1458
BsaWI WCCGGW 2 cut(s) 616, 727
Bsc4I CCNNNNNNNGG 3 cut(s) 280, 730, 1477
Bse1I ACTGG 1 cut(s) 227
Bse21I CCTNAGG 1 cut(s) 1248
Bse8I GATNNNNATC 2 cut(s) 66, 1029
BseAI TCCGGA 1 cut(s) 616
BseCI ATCGAT 1 cut(s) 374
BseDI CCNNGG 2 cut(s) 850, 1458
BseGI GGATG 4 cut(s) 10, 154, 454, 1258
BseJI GATNNNNATC 2 cut(s) 66, 1029
BseLI CCNNNNNNNGG 3 cut(s) 280, 730, 1477
BseMII CTCAG 6 cut(s) 227, 461, 888, 1143, 1239, 1353
BseNI ACTGG 1 cut(s) 227
BseRI GAGGAG 2 cut(s) 728, 1112
BseX3I CGGCCG 1 cut(s) 384
BseXI GCAGC 3 cut(s) 181, 310, 1200
Bsh1236I CGCG 2 cut(s) 458, 950
Bsh1285I CGRYCG 1 cut(s) 387
BshFI GGCC 5 cut(s) 386, 453, 959, 1424, 1470
BshVI ATCGAT 1 cut(s) 374
BsiEI CGRYCG 1 cut(s) 387
BsiHKAI GWGCWC 2 cut(s) 975, 1284
BsiHKCI CYCGRG 1 cut(s) 1310
BsiSI CCGG 4 cut(s) 274, 617, 728, 995
BsiWI CGTACG 1 cut(s) 576
BslFI GGGAC 1 cut(s) 462
BslI CCNNNNNNNGG 3 cut(s) 280, 730, 1477
BsmAI GTCTC 4 cut(s) 647, 734, 826, 1328
BsmBI CGTCTC 1 cut(s) 734
BsmFI GGGAC 1 cut(s) 462
BsnI GGCC 5 cut(s) 386, 453, 959, 1424, 1470
Bso31I GGTCTC 2 cut(s) 647, 826
BsoBI CYCGRG 1 cut(s) 1310
Bsp119I TTCGAA 2 cut(s) 921, 1070
Bsp1286I GDGCHC 2 cut(s) 975, 1284
Bsp13I TCCGGA 1 cut(s) 616
Bsp1720I GCTNAGC 1 cut(s) 874
BspACI CCGC 9 cut(s) 192, 342, 387, 393, 456, 485, 497, 500, 1216
BspANI GGCC 5 cut(s) 386, 453, 959, 1424, 1470
BspCNI CTCAG 6 cut(s) 226, 462, 887, 1144, 1240, 1354
BspDI ATCGAT 1 cut(s) 374
BspEI TCCGGA 1 cut(s) 616
BspFNI CGCG 2 cut(s) 458, 950
BspLI GGNNCC 3 cut(s) 272, 454, 993
BspMI ACCTGC 1 cut(s) 1198
BspPI GGATC 4 cut(s) 265, 278, 379, 1005
BspT104I TTCGAA 2 cut(s) 921, 1070
BspTI CTTAAG 1 cut(s) 1262
BspTNI GGTCTC 2 cut(s) 647, 826
BsrI ACTGG 1 cut(s) 227
BssECI CCNNGG 2 cut(s) 850, 1458
BssT1I CCWWGG 2 cut(s) 850, 1458
Bst4CI ACNGT 5 cut(s) 112, 725, 737, 745, 1143
Bst6I CTCTTC 2 cut(s) 75, 120
BstAFI CTTAAG 1 cut(s) 1262
BstAPI GCANNNNNTGC 1 cut(s) 1213
BstBI TTCGAA 2 cut(s) 921, 1070
BstC8I GCNNGC 3 cut(s) 199, 391, 779
BstDEI CTNAG 6 cut(s) 213, 470, 874, 1152, 1248, 1362
BstENI CCTNNNNNAGG 1 cut(s) 1475
BstF5I GGATG 4 cut(s) 10, 154, 454, 1258
BstFNI CGCG 2 cut(s) 458, 950
BstHHI GCGC 2 cut(s) 790, 1331
BstMAI GTCTC 4 cut(s) 647, 734, 826, 1328
BstMCI CGRYCG 1 cut(s) 387
BstMWI GCNNNNNNNGC 4 cut(s) 787, 956, 1111, 1213
BstNSI RCATGY 2 cut(s) 22, 1246
BstUI CGCG 2 cut(s) 458, 950
BstV1I GCAGC 3 cut(s) 181, 310, 1200
BstV2I GAAGAC 1 cut(s) 1059
BstX2I RGATCY 1 cut(s) 270
BstYI RGATCY 1 cut(s) 270
BstZI CGGCCG 1 cut(s) 384
Bsu15I ATCGAT 1 cut(s) 374
Bsu36I CCTNAGG 1 cut(s) 1248
BsuRI GGCC 5 cut(s) 386, 453, 959, 1424, 1470
BsuTUI ATCGAT 1 cut(s) 374
BtgZI GCGATG 1 cut(s) 1063
BtsCI GGATG 4 cut(s) 10, 154, 454, 1258
BtsI GCAGTG 1 cut(s) 797
BtsIMutI CAGTG 3 cut(s) 108, 156, 797
BveI ACCTGC 1 cut(s) 1198
Cac8I GCNNGC 3 cut(s) 199, 391, 779
CaiI CAGNNNCTG 2 cut(s) 1147, 1213
CfoI GCGC 2 cut(s) 790, 1331
Cfr13I GGNCC 4 cut(s) 452, 506, 957, 1422
ClaI ATCGAT 1 cut(s) 374
CseI GACGC 1 cut(s) 1247
Csp6I GTAC 8 cut(s) 22, 173, 544, 553, 577, 628, 708, 1303
CviAII CATG 4 cut(s) 19, 1182, 1243, 1295
CviQI GTAC 8 cut(s) 22, 173, 544, 553, 577, 628, 708, 1303
DdeI CTNAG 6 cut(s) 213, 470, 874, 1152, 1248, 1362
EaeI YGGCCR 1 cut(s) 384
EagI CGGCCG 1 cut(s) 384
Eam1104I CTCTTC 2 cut(s) 75, 120
EarI CTCTTC 2 cut(s) 75, 120
Ecl136II GAGCTC 1 cut(s) 973
EclXI CGGCCG 1 cut(s) 384
Eco130I CCWWGG 2 cut(s) 850, 1458
Eco24I GRGCYC 1 cut(s) 975
Eco31I GGTCTC 2 cut(s) 647, 826
Eco47I GGWCC 1 cut(s) 506
Eco52I CGGCCG 1 cut(s) 384
Eco53kI GAGCTC 1 cut(s) 973
Eco57I CTGAAG 2 cut(s) 180, 1031
Eco81I CCTNAGG 1 cut(s) 1248
Eco88I CYCGRG 1 cut(s) 1310
EcoICRI GAGCTC 1 cut(s) 973
EcoNI CCTNNNNNAGG 1 cut(s) 1475
EcoT14I CCWWGG 2 cut(s) 850, 1458
EcoT22I ATGCAT 1 cut(s) 1183
EcoT38I GRGCYC 1 cut(s) 975
ErhI CCWWGG 2 cut(s) 850, 1458
Esp3I CGTCTC 1 cut(s) 734
FaeI CATG 4 cut(s) 22, 1185, 1246, 1298
FalI AAGNNNNNCTT 2 cut(s) 1367, 1399
FaqI GGGAC 1 cut(s) 462
FatI CATG 4 cut(s) 18, 1181, 1242, 1294
FauI CCCGC 1 cut(s) 463
FauNDI CATATG 1 cut(s) 864
FbaI TGATCA 2 cut(s) 67, 231
Fnu4HI GCNGC 5 cut(s) 195, 299, 387, 498, 1214
FokI GGATG 4 cut(s) 17, 161, 461, 1265
FriOI GRGCYC 1 cut(s) 975
Fsp4HI GCNGC 5 cut(s) 195, 299, 387, 498, 1214
FspBI CTAG 1 cut(s) 936
FspI TGCGCA 1 cut(s) 789
GlaI GCGC 2 cut(s) 789, 1330
GluI GCNGC 5 cut(s) 195, 299, 387, 498, 1214
GsuI CTGGAG 1 cut(s) 439
HaeIII GGCC 5 cut(s) 386, 453, 959, 1424, 1470
HapII CCGG 4 cut(s) 274, 617, 728, 995
HgaI GACGC 1 cut(s) 1247
HhaI GCGC 2 cut(s) 790, 1331
Hin1II CATG 4 cut(s) 22, 1185, 1246, 1298
Hin6I GCGC 2 cut(s) 788, 1329
HinP1I GCGC 2 cut(s) 788, 1329
HincII GTYRAC 1 cut(s) 805
HindII GTYRAC 1 cut(s) 805
HindIII AAGCTT 1 cut(s) 137
HinfI GANTC 4 cut(s) 86, 718, 838, 1088
HpaI GTTAAC 1 cut(s) 805
HpaII CCGG 4 cut(s) 274, 617, 728, 995
HphI GGTGA 3 cut(s) 713, 757, 851
Hpy166II GTNNAC 3 cut(s) 733, 805, 1483
Hpy188I TCNGA 7 cut(s) 117, 321, 843, 1002, 1153, 1363, 1477
Hpy188III TCNNGA 6 cut(s) 143, 479, 509, 617, 1312, 1441
Hpy8I GTNNAC 3 cut(s) 733, 805, 1483
Hpy99I CGWCG 8 cut(s) 29, 32, 35, 56, 59, 62, 248, 584
HpyAV CCTTC 2 cut(s) 1006, 1066
HpyCH4III ACNGT 5 cut(s) 112, 725, 737, 745, 1143
HpyCH4IV ACGT 2 cut(s) 228, 579
HpyCH4V TGCA 5 cut(s) 828, 1181, 1288, 1298, 1345
HpyF10VI GCNNNNNNNGC 4 cut(s) 787, 956, 1111, 1213
HpyF3I CTNAG 6 cut(s) 213, 470, 874, 1152, 1248, 1362
HpySE526I ACGT 2 cut(s) 228, 579
Hsp92II CATG 4 cut(s) 22, 1185, 1246, 1298
HspAI GCGC 2 cut(s) 788, 1329
Kpn2I TCCGGA 1 cut(s) 616
Ksp22I TGATCA 2 cut(s) 67, 231
KspAI GTTAAC 1 cut(s) 805
LmnI GCTCC 6 cut(s) 295, 619, 883, 991, 1123, 1221
Lsp1109I GCAGC 3 cut(s) 181, 310, 1200
LweI GCATC 3 cut(s) 139, 456, 1168
MaeI CTAG 1 cut(s) 936
MaeII ACGT 2 cut(s) 228, 579
MaeIII GTNAC 6 cut(s) 47, 425, 719, 857, 944, 1450
MboII GAAGA 9 cut(s) 92, 134, 137, 275, 775, 856, 1015, 1025, 1059
MflI RGATCY 1 cut(s) 270
MhlI GDGCHC 2 cut(s) 975, 1284
MluCI AATT 7 cut(s) 311, 703, 917, 1165, 1194, 1340, 1370
MluI ACGCGT 1 cut(s) 948
MlyI GAGTC 2 cut(s) 727, 1097
MmeI TCCRAC 2 cut(s) 273, 963
Mph1103I ATGCAT 1 cut(s) 1183
MroI TCCGGA 1 cut(s) 616
MseI TTAA 4 cut(s) 804, 1263, 1373, 1400
MslI CAYNNNNRTG 2 cut(s) 161, 1041
MspA1I CMGCKG 1 cut(s) 194
MspCI CTTAAG 1 cut(s) 1262
MspI CCGG 4 cut(s) 274, 617, 728, 995
MvnI CGCG 2 cut(s) 458, 950
MwoI GCNNNNNNNGC 4 cut(s) 787, 956, 1111, 1213
NdeI CATATG 1 cut(s) 864
NlaIII CATG 4 cut(s) 22, 1185, 1246, 1298
NlaIV GGNNCC 3 cut(s) 272, 454, 993
NmeAIII GCCGAG 1 cut(s) 1357
NmuCI GTSAC 3 cut(s) 47, 719, 857
NsbI TGCGCA 1 cut(s) 789
NsiI ATGCAT 1 cut(s) 1183
NspI RCATGY 2 cut(s) 22, 1246
NspV TTCGAA 2 cut(s) 921, 1070
OliI CACNNNNGTG 1 cut(s) 161
PaeR7I CTCGAG 1 cut(s) 1310
PaqCI CACCTGC 1 cut(s) 1198
PciI ACATGT 1 cut(s) 18
PcsI WCGNNNNNNNCGW 2 cut(s) 39, 57
PfeI GAWTC 2 cut(s) 86, 838
Pfl23II CGTACG 1 cut(s) 576
PkrI GCNGC 5 cut(s) 196, 300, 388, 499, 1215
PleI GAGTC 2 cut(s) 726, 1096
PpsI GAGTC 2 cut(s) 726, 1096
PscI ACATGT 1 cut(s) 18
Psp124BI GAGCTC 1 cut(s) 975
PspLI CGTACG 1 cut(s) 576
PspN4I GGNNCC 3 cut(s) 272, 454, 993
PspPI GGNCC 4 cut(s) 452, 506, 957, 1422
PstNI CAGNNNCTG 2 cut(s) 1147, 1213
PsuI RGATCY 1 cut(s) 270
RsaI GTAC 8 cut(s) 23, 174, 545, 554, 578, 629, 709, 1304
RsaNI GTAC 8 cut(s) 22, 173, 544, 553, 577, 628, 708, 1303
RseI CAYNNNNRTG 2 cut(s) 161, 1041
SacI GAGCTC 1 cut(s) 975
SaqAI TTAA 4 cut(s) 804, 1263, 1373, 1400
SatI GCNGC 5 cut(s) 195, 299, 387, 498, 1214
Sau96I GGNCC 4 cut(s) 452, 506, 957, 1422
SchI GAGTC 2 cut(s) 727, 1097
SduI GDGCHC 2 cut(s) 975, 1284
SfaNI GCATC 3 cut(s) 139, 456, 1168
Sfr274I CTCGAG 1 cut(s) 1310
SfuI TTCGAA 2 cut(s) 921, 1070
SinI GGWCC 1 cut(s) 506
SlaI CTCGAG 1 cut(s) 1310
SmiMI CAYNNNNRTG 2 cut(s) 161, 1041
SmlI CTYRAG 3 cut(s) 974, 1262, 1310
SmoI CTYRAG 3 cut(s) 974, 1262, 1310
Sse9I AATT 7 cut(s) 311, 703, 917, 1165, 1194, 1340, 1370
SsiI CCGC 9 cut(s) 192, 342, 387, 393, 456, 485, 497, 500, 1216
SspMI CTAG 1 cut(s) 936
SstI GAGCTC 1 cut(s) 975
StyI CCWWGG 2 cut(s) 850, 1458
TaaI ACNGT 5 cut(s) 112, 725, 737, 745, 1143
TaiI ACGT 2 cut(s) 231, 582
TaqI TCGA 9 cut(s) 74, 374, 533, 582, 921, 1070, 1086, 1311, 1407
TaqII GACCGA 2 cut(s) 511, 850
TasI AATT 7 cut(s) 311, 703, 917, 1165, 1194, 1340, 1370
TatI WGTACW 2 cut(s) 172, 1302
TauI GCSGC 2 cut(s) 389, 500
TfiI GAWTC 2 cut(s) 86, 838
Tru1I TTAA 4 cut(s) 804, 1263, 1373, 1400
Tru9I TTAA 4 cut(s) 804, 1263, 1373, 1400
TscAI CASTG 3 cut(s) 115, 163, 797
TseFI GTSAC 3 cut(s) 47, 719, 857
TseI GCWGC 3 cut(s) 194, 298, 1213
Tsp45I GTSAC 3 cut(s) 47, 719, 857
TspDTI ATGAA 4 cut(s) 276, 776, 1025, 1159
TspGWI ACGGA 1 cut(s) 725
TspRI CASTG 3 cut(s) 115, 163, 797
Vha464I CTTAAG 1 cut(s) 1262
VpaK11BI GGWCC 1 cut(s) 506
XagI CCTNNNNNAGG 1 cut(s) 1475
XapI RAATTY 1 cut(s) 917
XceI RCATGY 2 cut(s) 22, 1246
XcmI CCANNNNNNNNNTGG 1 cut(s) 1117
XhoI CTCGAG 1 cut(s) 1310
XspI CTAG 1 cut(s) 936
Zsp2I ATGCAT 1 cut(s) 1183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.