FvH4_2g40470

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000864
Physical Location & Seq
Forward (+)
28811195 .. 28813153
1959 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g40470.t1

Sequence Viewer

Length: 1473 bp
ATGGACGACGAGTCAGACTATATGTACGATGATGAGGAATCAAATGATCAGGAAGATTACGATGAGGAATCAAATGATCAGGAAGATTACACTGTTCTGAAAGAGGAAGAGATTGCCAAACTTCAAGAGGATGATATCAATGAAGTGTCTAATGTCCTCTGTATATCGCAATCAGCTGCCTGTTTTGTCCTGACTCAATTCAACTGGAAAGTGATCGACCTCAAAGACGACTGGTTCGCCGACGAAGACGGAATCCGGCAAAAAATGGGTCTGTTGGAGAAGCCTGTGGTTCAATTTGCGGAAAAGCAAAGAAGTATTTGCGGGATATGTTTCGATGGTTACAACTATGGATGGATGTATTGGGCTGCGTGCGGTCATCCTTATTGTGGACAGTGCTGGAGGGGTTACGTCAGCGCAGCGATCAAGGATGGTCCGCTGTGTTTGATGCTGAGGTGTCCTGAACCGTCGTGCAGGGCGGCGGTTGGTCGTGACTTGGTCTTGGTGTTGGTGTCGAAACAGGAGTACGAGAGGTACAGGTACTATCTGTTGAGATCTTATGTCGAACAGCACCTCAAGATCAAGTGGTGTCCTGCTCCGGGTTGTAAGTACGCTGTTAAGGTTGATAGCTTTGCGAACAGAGGTTATGATGTTTCGTGTCTTTGTTCGAATAGCTATTGCTGGAATTGTACGGAGGAGAATCACCGTCCGGTGGATTGTGAGACGGTGAGGAAGTGGATCATGAAGAACAAGGATAGCTCGCAAAATGCGGAGTGGATATTGGTTAACACCAAGCCATGTCCAAAATGCAAGAGACCAATTCAGAAGAACCAAGGGTGTCATCATATGACTTGCTCAGCTCCTTGTAGGTATGAATTTTGCTGGTATTGTCTTAAGAAATTCGAGAGCTACTCTGCTGCATGTTGCAATGCGTATAGGGGAAATTATGATGAGATGAAGGAGTTGAAGTTGGAGCCGGATAAGAAGAAGCTGAAGCATCATTTAGATAGGTACGTTCATTATTACGAGCGATGGGCAAGCAATCGGACTTCGAAGGAAAAGGCCATCGAGTCTTTGAATGAGGTGAGAACCAAGCACATTGAGGAGCTTGTTAGCAAACAACTGTATCTGACTAAGCTTGAACTCATTTTCATCATAGAGGCATGGCAACAGATAATTGAATGTAGGCAGGTACTGCGGTGGAGCTATGCGTATGGCTACTACATGCCTGAGGATGAGCCTCTTAAGGCAAAGCTGTTTGAGCACTTACAAGGTCACGCAGAAAACAGGCTTGAGAGGCTTCACAAATGCGCCGTGACTGGATTGCAGCCATTCCTTTCTTCTGAGGAGGAACTAAAGGATAGTTTCAACAAATTTCGCATTGAACTTGATGCACTGACAAGGGCCACTAAAGATCATTTCAAGAAGCTGGTTACTGGCTTGGAGAATGGCTTATCGGAGGTGGATGGCGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IBR zf-RanBP

Protein Analysis

491

Amino Acids

57.59

Weight (kDa)

5.49

Isoelectric Point (pI)

56.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UBA_ARI1 PF21235 41 - 74 9.5e-07 E3 ubiquitin-protein ligase ARIH1, UBA-like domain
IBR PF01485 176 - 239 6.2e-15 IBR domain, a half RING-finger domain
IBR_2 PF22605 259 - 301 3.3e-11 IBR domain
IBR_1 PF22191 262 - 301 1e-11 IBR domain
Ariadne PF19422 336 - 483 8.5e-06 Ariadne domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1177
AciI CCGC 8 cut(s) 299, 321, 372, 434, 476, 479, 767, 1195
AclWI GGATC 1 cut(s) 743
AcsI RAATTY 3 cut(s) 872, 896, 1370
AcuI CTGAAG 1 cut(s) 1010
AfaI GTAC 8 cut(s) 26, 524, 533, 539, 608, 688, 1010, 1191
AfiI CCNNNNNNNGG 3 cut(s) 386, 596, 709
AflII CTTAAG 2 cut(s) 890, 1241
AhdI GACNNNNNGTC 1 cut(s) 10
Alw21I GWGCWC 1 cut(s) 1263
Alw26I GTCTC 2 cut(s) 713, 805
AlwI GGATC 1 cut(s) 743
AlwNI CAGNNNCTG 1 cut(s) 1192
AoxI GGCC 2 cut(s) 1059, 1401
ApeKI GCWGC 5 cut(s) 176, 365, 416, 914, 1324
ApoI RAATTY 3 cut(s) 872, 896, 1370
ArsI GACNNNNNNTTYG 4 cut(s) 218, 250, 253, 285
AspLEI GCGC 2 cut(s) 416, 1310
AspS9I GGNCC 2 cut(s) 431, 1401
AsuC2I CCSGG 1 cut(s) 597
AsuHPI GGTGA 3 cut(s) 692, 736, 1093
AsuII TTCGAA 2 cut(s) 665, 1049
AvaII GGWCC 1 cut(s) 431
AxyI CCTNAGG 1 cut(s) 1227
BaeI ACNNNNGTAYC 4 cut(s) 1106, 1139, 1181, 1214
BbsI GAAGAC 1 cut(s) 252
Bbv12I GWGCWC 1 cut(s) 1263
BbvCI CCTCAGC 1 cut(s) 449
BbvI GCAGC 5 cut(s) 163, 352, 428, 901, 1336
BccI CCATC 6 cut(s) 329, 345, 422, 1023, 1070, 1457
BceAI ACGGC 1 cut(s) 1295
BclI TGATCA 2 cut(s) 46, 76
BcnI CCSGG 1 cut(s) 597
BcoDI GTCTC 2 cut(s) 713, 805
BfrI CTTAAG 2 cut(s) 890, 1241
BfuAI ACCTGC 1 cut(s) 1177
BglII AGATCT 1 cut(s) 551
BisI GCNGC 6 cut(s) 177, 366, 417, 477, 915, 1325
BlpI GCTNAGC 1 cut(s) 853
BlsI GCNGC 6 cut(s) 178, 367, 418, 478, 916, 1326
Bme1390I CCNGG 1 cut(s) 597
Bme18I GGWCC 1 cut(s) 431
BmeRI GACNNNNNGTC 1 cut(s) 10
BmgT120I GGNCC 2 cut(s) 431, 1401
BmiI GGNNCC 1 cut(s) 972
BmrFI CCNGG 1 cut(s) 597
BmsI GCATC 3 cut(s) 435, 1003, 1378
BpiI GAAGAC 1 cut(s) 252
BplI GAGNNNNNCTC 2 cut(s) 893, 925
BpmI CTGGAG 1 cut(s) 418
Bpu10I CCTNAGC 1 cut(s) 449
Bpu1102I GCTNAGC 1 cut(s) 853
Bpu14I TTCGAA 2 cut(s) 665, 1049
BpuEI CTTGAG 2 cut(s) 557, 1310
BpuMI CCSGG 1 cut(s) 597
BsaI GGTCTC 1 cut(s) 805
BsaJI CCNNGG 1 cut(s) 829
BsaWI WCCGGW 1 cut(s) 706
BsaXI ACNNNNNCTCC 2 cut(s) 950, 980
Bsc4I CCNNNNNNNGG 3 cut(s) 386, 596, 709
Bse1I ACTGG 4 cut(s) 209, 236, 1321, 1438
Bse21I CCTNAGG 1 cut(s) 1227
Bse3DI GCAATG 1 cut(s) 931
BseDI CCNNGG 1 cut(s) 829
BseGI GGATG 7 cut(s) 136, 356, 360, 376, 433, 1237, 1468
BseLI CCNNNNNNNGG 3 cut(s) 386, 596, 709
BseMI GCAATG 1 cut(s) 931
BseMII CTCAG 4 cut(s) 440, 867, 1218, 1332
BseNI ACTGG 4 cut(s) 209, 236, 1321, 1438
BseRI GAGGAG 3 cut(s) 707, 1115, 1358
BseXI GCAGC 5 cut(s) 163, 352, 428, 901, 1336
BsgI GTGCAG 1 cut(s) 490
BshFI GGCC 2 cut(s) 1061, 1403
BsiHKAI GWGCWC 1 cut(s) 1263
BsiSI CCGG 4 cut(s) 256, 596, 707, 974
BslI CCNNNNNNNGG 3 cut(s) 386, 596, 709
BsmAI GTCTC 2 cut(s) 713, 805
BsmBI CGTCTC 1 cut(s) 713
BsnI GGCC 2 cut(s) 1061, 1403
Bso31I GGTCTC 1 cut(s) 805
Bsp119I TTCGAA 2 cut(s) 665, 1049
Bsp1286I GDGCHC 1 cut(s) 1263
Bsp143I GATC 8 cut(s) 46, 76, 213, 420, 551, 576, 735, 1411
Bsp1720I GCTNAGC 1 cut(s) 853
BspACI CCGC 8 cut(s) 299, 321, 372, 434, 476, 479, 767, 1195
BspANI GGCC 2 cut(s) 1061, 1403
BspCNI CTCAG 4 cut(s) 441, 866, 1219, 1333
BspHI TCATGA 1 cut(s) 738
BspLI GGNNCC 1 cut(s) 972
BspMI ACCTGC 1 cut(s) 1177
BspPI GGATC 1 cut(s) 743
BspT104I TTCGAA 2 cut(s) 665, 1049
BspTI CTTAAG 2 cut(s) 890, 1241
BspTNI GGTCTC 1 cut(s) 805
BsrDI GCAATG 1 cut(s) 931
BsrI ACTGG 4 cut(s) 209, 236, 1321, 1438
BssECI CCNNGG 1 cut(s) 829
BssMI GATC 8 cut(s) 46, 76, 213, 420, 551, 576, 735, 1411
BssT1I CCWWGG 1 cut(s) 829
Bst4CI ACNGT 6 cut(s) 94, 393, 465, 704, 724, 1122
Bst6I CTCTTC 1 cut(s) 102
BstAFI CTTAAG 2 cut(s) 890, 1241
BstAPI GCANNNNNTGC 1 cut(s) 1192
BstBI TTCGAA 2 cut(s) 665, 1049
BstC8I GCNNGC 3 cut(s) 370, 758, 1036
BstDEI CTNAG 5 cut(s) 449, 853, 1131, 1227, 1341
BstF5I GGATG 7 cut(s) 136, 356, 360, 376, 433, 1237, 1468
BstHHI GCGC 2 cut(s) 416, 1310
BstKTI GATC 8 cut(s) 49, 79, 216, 423, 554, 579, 738, 1414
BstMAI GTCTC 2 cut(s) 713, 805
BstMBI GATC 8 cut(s) 46, 76, 213, 420, 551, 576, 735, 1411
BstMWI GCNNNNNNNGC 3 cut(s) 1192, 1258, 1294
BstNSI RCATGY 2 cut(s) 921, 1225
BstSCI CCNGG 1 cut(s) 595
BstV1I GCAGC 5 cut(s) 163, 352, 428, 901, 1336
BstV2I GAAGAC 1 cut(s) 252
BstX2I RGATCY 1 cut(s) 551
BstYI RGATCY 1 cut(s) 551
Bsu36I CCTNAGG 1 cut(s) 1227
BsuRI GGCC 2 cut(s) 1061, 1403
BtgZI GCGATG 1 cut(s) 1042
BtsCI GGATG 7 cut(s) 136, 356, 360, 376, 433, 1237, 1468
BtsIMutI CAGTG 3 cut(s) 90, 398, 1391
BveI ACCTGC 1 cut(s) 1177
Cac8I GCNNGC 3 cut(s) 370, 758, 1036
CaiI CAGNNNCTG 1 cut(s) 1192
CciI TCATGA 1 cut(s) 738
CfoI GCGC 2 cut(s) 416, 1310
Cfr13I GGNCC 2 cut(s) 431, 1401
Csp6I GTAC 8 cut(s) 25, 523, 532, 538, 607, 687, 1009, 1190
CviAII CATG 5 cut(s) 739, 795, 918, 1161, 1222
CviQI GTAC 8 cut(s) 25, 523, 532, 538, 607, 687, 1009, 1190
DdeI CTNAG 5 cut(s) 449, 853, 1131, 1227, 1341
DpnI GATC 8 cut(s) 48, 78, 215, 422, 553, 578, 737, 1413
DpnII GATC 8 cut(s) 46, 76, 213, 420, 551, 576, 735, 1411
DriI GACNNNNNGTC 1 cut(s) 10
Eam1104I CTCTTC 1 cut(s) 102
Eam1105I GACNNNNNGTC 1 cut(s) 10
EarI CTCTTC 1 cut(s) 102
Eco130I CCWWGG 1 cut(s) 829
Eco31I GGTCTC 1 cut(s) 805
Eco32I GATATC 1 cut(s) 136
Eco47I GGWCC 1 cut(s) 431
Eco57I CTGAAG 1 cut(s) 1010
Eco81I CCTNAGG 1 cut(s) 1227
EcoRV GATATC 1 cut(s) 136
EcoT14I CCWWGG 1 cut(s) 829
ErhI CCWWGG 1 cut(s) 829
Esp3I CGTCTC 1 cut(s) 713
FaeI CATG 5 cut(s) 742, 798, 921, 1164, 1225
FatI CATG 5 cut(s) 738, 794, 917, 1160, 1221
FauI CCCGC 1 cut(s) 314
FauNDI CATATG 1 cut(s) 843
FbaI TGATCA 2 cut(s) 46, 76
Fnu4HI GCNGC 6 cut(s) 177, 366, 417, 477, 915, 1325
FokI GGATG 6 cut(s) 143, 363, 363, 367, 440, 1244
Fsp4HI GCNGC 6 cut(s) 177, 366, 417, 477, 915, 1325
GlaI GCGC 2 cut(s) 415, 1309
GluI GCNGC 6 cut(s) 177, 366, 417, 477, 915, 1325
GsuI CTGGAG 1 cut(s) 418
HaeIII GGCC 2 cut(s) 1061, 1403
HapII CCGG 4 cut(s) 256, 596, 707, 974
HhaI GCGC 2 cut(s) 416, 1310
Hin1II CATG 5 cut(s) 742, 798, 921, 1164, 1225
Hin6I GCGC 2 cut(s) 414, 1308
HinP1I GCGC 2 cut(s) 414, 1308
HincII GTYRAC 1 cut(s) 784
HindII GTYRAC 1 cut(s) 784
HindIII AAGCTT 1 cut(s) 1133
HinfI GANTC 7 cut(s) 11, 38, 68, 193, 252, 697, 1067
HpaI GTTAAC 1 cut(s) 784
HpaII CCGG 4 cut(s) 256, 596, 707, 974
HphI GGTGA 3 cut(s) 692, 736, 1093
Hpy166II GTNNAC 2 cut(s) 389, 784
Hpy188I TCNGA 7 cut(s) 16, 99, 822, 1044, 1128, 1342, 1456
Hpy8I GTNNAC 2 cut(s) 389, 784
Hpy99I CGWCG 3 cut(s) 11, 245, 469
HpyAV CCTTC 2 cut(s) 949, 1045
HpyCH4III ACNGT 6 cut(s) 94, 393, 465, 704, 724, 1122
HpyCH4IV ACGT 2 cut(s) 408, 1011
HpyCH4V TGCA 6 cut(s) 471, 807, 917, 924, 1324, 1391
HpyF10VI GCNNNNNNNGC 3 cut(s) 1192, 1258, 1294
HpyF3I CTNAG 5 cut(s) 449, 853, 1131, 1227, 1341
HpySE526I ACGT 2 cut(s) 408, 1011
Hsp92II CATG 5 cut(s) 742, 798, 921, 1164, 1225
HspAI GCGC 2 cut(s) 414, 1308
Ksp22I TGATCA 2 cut(s) 46, 76
KspAI GTTAAC 1 cut(s) 784
Kzo9I GATC 8 cut(s) 46, 76, 213, 420, 551, 576, 735, 1411
LmnI GCTCC 5 cut(s) 598, 862, 970, 1102, 1200
Lsp1109I GCAGC 5 cut(s) 163, 352, 428, 901, 1336
LweI GCATC 3 cut(s) 435, 1003, 1378
MaeII ACGT 2 cut(s) 408, 1011
MaeIII GTNAC 6 cut(s) 338, 404, 488, 1271, 1312, 1429
MalI GATC 8 cut(s) 48, 78, 215, 422, 553, 578, 737, 1413
MboI GATC 8 cut(s) 46, 76, 213, 420, 551, 576, 735, 1411
MboII GAAGA 8 cut(s) 65, 95, 119, 257, 754, 835, 994, 1329
MflI RGATCY 1 cut(s) 551
MhlI GDGCHC 1 cut(s) 1263
MluCI AATT 9 cut(s) 197, 293, 682, 816, 872, 896, 940, 1173, 1370
MlyI GAGTC 3 cut(s) 20, 187, 1076
MmeI TCCRAC 2 cut(s) 255, 948
MseI TTAA 4 cut(s) 615, 783, 891, 1242
MspA1I CMGCKG 2 cut(s) 176, 436
MspCI CTTAAG 2 cut(s) 890, 1241
MspI CCGG 4 cut(s) 256, 596, 707, 974
MspR9I CCNGG 1 cut(s) 597
MwoI GCNNNNNNNGC 3 cut(s) 1192, 1258, 1294
NciI CCSGG 1 cut(s) 597
NdeI CATATG 1 cut(s) 843
NdeII GATC 8 cut(s) 46, 76, 213, 420, 551, 576, 735, 1411
NlaIII CATG 5 cut(s) 742, 798, 921, 1164, 1225
NlaIV GGNNCC 1 cut(s) 972
NmuCI GTSAC 3 cut(s) 488, 1271, 1312
NspI RCATGY 2 cut(s) 921, 1225
NspV TTCGAA 2 cut(s) 665, 1049
PagI TCATGA 1 cut(s) 738
PfeI GAWTC 4 cut(s) 38, 68, 252, 697
PflFI GACNNNGTC 1 cut(s) 494
PkrI GCNGC 6 cut(s) 178, 367, 418, 478, 916, 1326
PleI GAGTC 3 cut(s) 19, 187, 1075
PpsI GAGTC 3 cut(s) 19, 187, 1075
PspN4I GGNNCC 1 cut(s) 972
PspPI GGNCC 2 cut(s) 431, 1401
PstNI CAGNNNCTG 1 cut(s) 1192
PsuI RGATCY 1 cut(s) 551
PsyI GACNNNGTC 1 cut(s) 494
PvuII CAGCTG 1 cut(s) 176
RsaI GTAC 8 cut(s) 26, 524, 533, 539, 608, 688, 1010, 1191
RsaNI GTAC 8 cut(s) 25, 523, 532, 538, 607, 687, 1009, 1190
SaqAI TTAA 4 cut(s) 615, 783, 891, 1242
SatI GCNGC 6 cut(s) 177, 366, 417, 477, 915, 1325
Sau3AI GATC 8 cut(s) 46, 76, 213, 420, 551, 576, 735, 1411
Sau96I GGNCC 2 cut(s) 431, 1401
SchI GAGTC 3 cut(s) 20, 187, 1076
ScrFI CCNGG 1 cut(s) 597
SduI GDGCHC 1 cut(s) 1263
SfaNI GCATC 3 cut(s) 435, 1003, 1378
SfuI TTCGAA 2 cut(s) 665, 1049
SinI GGWCC 1 cut(s) 431
SmlI CTYRAG 4 cut(s) 572, 890, 1241, 1289
SmoI CTYRAG 4 cut(s) 572, 890, 1241, 1289
Sse9I AATT 9 cut(s) 197, 293, 682, 816, 872, 896, 940, 1173, 1370
SsiI CCGC 8 cut(s) 299, 321, 372, 434, 476, 479, 767, 1195
StyD4I CCNGG 1 cut(s) 595
StyI CCWWGG 1 cut(s) 829
TaaI ACNGT 6 cut(s) 94, 393, 465, 704, 724, 1122
TaiI ACGT 2 cut(s) 411, 1014
TaqI TCGA 8 cut(s) 216, 333, 512, 561, 665, 900, 1049, 1065
TasI AATT 9 cut(s) 197, 293, 682, 816, 872, 896, 940, 1173, 1370
TauI GCSGC 1 cut(s) 479
TfiI GAWTC 4 cut(s) 38, 68, 252, 697
Tru1I TTAA 4 cut(s) 615, 783, 891, 1242
Tru9I TTAA 4 cut(s) 615, 783, 891, 1242
TscAI CASTG 3 cut(s) 97, 398, 1398
TseFI GTSAC 3 cut(s) 488, 1271, 1312
TseI GCWGC 5 cut(s) 176, 365, 416, 914, 1324
Tsp45I GTSAC 3 cut(s) 488, 1271, 1312
TspDTI ATGAA 6 cut(s) 156, 755, 885, 968, 1004, 1138
TspGWI ACGGA 2 cut(s) 264, 704
TspRI CASTG 3 cut(s) 97, 398, 1398
Tth111I GACNNNGTC 1 cut(s) 494
Vha464I CTTAAG 2 cut(s) 890, 1241
VpaK11BI GGWCC 1 cut(s) 431
XapI RAATTY 3 cut(s) 872, 896, 1370
XceI RCATGY 2 cut(s) 921, 1225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.