FvH4_2g26880

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000978
Physical Location & Seq
Reverse (-)
21505013 .. 21507404
2392 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g26880.t1

Sequence Viewer

Length: 1209 bp
ATGATCTCCATCTCCACTATGGACGACTATCCTGCTAGAGTTGCTATTTCTCTGTTCTGCTCCTATATACTTCTGTTGCGAATTACCTGTAGTACTGTAAGCCCTGCCTTGGCTTTGGAAGACTGCGAGTTTCCAGCCATCTTCAACTTCGGAGACTCAAATTCAGATACTGGTGGATTGGCAGCATCCTTCTACCCCCCATCTACACCTTTTGGAGAGACCTATTTCCATATGCCGGTCGGAAGATTCTCAGATGGAAGACTCATAGTCGATTTCCTCGCTAAGAGTCTAAGTCGCCCTTATCTAAGCGCATACCTGGATTCCCTGGGGACCAATTTCTCTCATGGTGCAAATTTTGCTACTTGGGCTTCCACTATCAGACTCCCACATCTCATTTTACCACTAGCCGATGGATATAGTCCCTTCTACCTTGATATTCAATATCTGCAGTTCCTGCAACTAAAATCCAGATCACAACTCATAAGGCAGCGAGGGGGAATATATGCAAGTTTAATGCCCGAGGAGGAGTACTTCTCCAAAGCTTTGTACACATTCGATATTGGTCAGAATGATCTTGGCGAGGGAATTTTCGGTAACAAGACTATACAGGAAGTCAGTGCATCTGTACCTGATATAATCCCTGGTTTCTCAGCAAATATTAAGAAAATATATGACTTGGGGGCAAGAACATTTTGGATCCACAATACGGGGCCGATTGGCTGTCTCCCTTACGTTTTGGTAAGATTCCCAGCAGCTGATAAAGATGAGGCTGGCTGTGCAAAGGCTTACAACGAAGTATGTCAACATTTTAACCAGGAGCTGAAGCAGGCCATAGTTCAACTCAGGAAGGATCTTCCCTTGGCTGCATTCACTTCTGTAGATGTGTATTCTGTCAAGTACTCTCTTTTCAAGGAACCCCAAAAGTATGGATTTGAGCTCCCACTTGTTTCTTGTTGTGGCTATGGTGGTAAGTATAATTATAACAGTAGTGCAGGATGTGGAACAACAATTACAGTCAATGGAAGCCAAATCTTTGTTGGTTCATGCGAAAACCCCTCAACTAGATTGAACTGGGATGGGATTCACTACACCGAGGCAGCTGCAAAATTTGTTTTTGATAAAATCTCAACTGGAGCATATTCAGATCCACCCCTGCCATTGAAACAAGCATGCCACAGTAGTCGAAACAAGAACTTGGAGAAAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005488 GO:0006950 GO:0006952 GO:0008150 GO:0016787 GO:0016788 GO:0019863 GO:0019865 GO:0044877 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

403

Amino Acids

44.42

Weight (kDa)

6.0

Isoelectric Point (pI)

40.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 47 - 375 7.4e-44 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 981
AclWI GGATC 4 cut(s) 691, 704, 858, 1139
AcsI RAATTY 4 cut(s) 160, 352, 585, 1106
AcuI CTGAAG 1 cut(s) 842
AfaI GTAC 5 cut(s) 94, 530, 548, 627, 899
AfiI CCNNNNNNNGG 3 cut(s) 109, 235, 706
AgsI TTSAA 6 cut(s) 145, 440, 839, 910, 1069, 1162
AhdI GACNNNNNGTC 1 cut(s) 266
AjnI CCWGG 4 cut(s) 315, 324, 640, 813
AluBI AGCT 5 cut(s) 542, 755, 820, 937, 1100
AluI AGCT 5 cut(s) 542, 755, 820, 937, 1100
Alw21I GWGCWC 1 cut(s) 939
Alw26I GTCTC 3 cut(s) 147, 212, 728
AlwI GGATC 4 cut(s) 691, 704, 858, 1139
AlwNI CAGNNNCTG 4 cut(s) 170, 454, 755, 820
Ama87I CYCGRG 1 cut(s) 518
AoxI GGCC 2 cut(s) 710, 828
ApeKI GCWGC 6 cut(s) 182, 487, 752, 863, 1097, 1100
ApoI RAATTY 4 cut(s) 160, 352, 585, 1106
AspLEI GCGC 1 cut(s) 311
AspS9I GGNCC 2 cut(s) 330, 710
AvaI CYCGRG 1 cut(s) 518
AvaII GGWCC 1 cut(s) 330
BamHI GGATCC 1 cut(s) 696
BanII GRGCYC 1 cut(s) 939
BbsI GAAGAC 2 cut(s) 126, 265
Bbv12I GWGCWC 1 cut(s) 939
BbvI GCAGC 6 cut(s) 194, 499, 764, 850, 1087, 1109
BccI CCATC 6 cut(s) 17, 146, 208, 248, 404, 1070
BcgI CGANNNNNNTGC 4 cut(s) 14, 48, 1082, 1116
BciT130I CCWGG 4 cut(s) 317, 326, 642, 815
BcoDI GTCTC 3 cut(s) 147, 212, 728
BfaI CTAG 3 cut(s) 36, 404, 1062
BfmI CTRYAG 3 cut(s) 88, 446, 876
BisI GCNGC 6 cut(s) 183, 488, 753, 864, 1098, 1101
BlsI GCNGC 6 cut(s) 184, 489, 754, 865, 1099, 1102
BmcAI AGTACT 3 cut(s) 94, 530, 899
Bme1390I CCNGG 4 cut(s) 317, 326, 642, 815
Bme18I GGWCC 1 cut(s) 330
BmeRI GACNNNNNGTC 1 cut(s) 266
BmeT110I CYCGRG 1 cut(s) 518
BmgT120I GGNCC 2 cut(s) 330, 710
BmiI GGNNCC 4 cut(s) 331, 698, 711, 915
BmrFI CCNGG 4 cut(s) 317, 326, 642, 815
BmrI ACTGGG 1 cut(s) 1081
BmsI GCATC 2 cut(s) 194, 629
BmuI ACTGGG 1 cut(s) 1081
BpiI GAAGAC 2 cut(s) 126, 265
BplI GAGNNNNNCTC 2 cut(s) 518, 550
BpmI CTGGAG 1 cut(s) 1152
BsaBI GATNNNNATC 1 cut(s) 8
BsaI GGTCTC 1 cut(s) 212
BsaJI CCNNGG 7 cut(s) 108, 324, 325, 519, 640, 858, 1092
Bsc4I CCNNNNNNNGG 3 cut(s) 109, 235, 706
Bse118I RCCGGY 1 cut(s) 235
Bse1I ACTGG 3 cut(s) 175, 1076, 1135
Bse8I GATNNNNATC 1 cut(s) 8
BseBI CCWGG 4 cut(s) 317, 326, 642, 815
BseDI CCNNGG 7 cut(s) 108, 324, 325, 519, 640, 858, 1092
BseGI GGATG 3 cut(s) 185, 1001, 1081
BseJI GATNNNNATC 1 cut(s) 8
BseLI CCNNNNNNNGG 3 cut(s) 109, 235, 706
BseMII CTCAG 3 cut(s) 264, 663, 856
BseNI ACTGG 3 cut(s) 175, 1076, 1135
BseRI GAGGAG 2 cut(s) 536, 539
BseXI GCAGC 6 cut(s) 194, 499, 764, 850, 1087, 1109
BseYI CCCAGC 1 cut(s) 748
BsgI GTGCAG 1 cut(s) 1011
Bsh1285I CGRYCG 1 cut(s) 240
BshFI GGCC 2 cut(s) 712, 830
BsiEI CGRYCG 1 cut(s) 240
BsiHKAI GWGCWC 1 cut(s) 939
BsiHKCI CYCGRG 1 cut(s) 518
BsiSI CCGG 1 cut(s) 236
BslFI GGGAC 2 cut(s) 343, 405
BslI CCNNNNNNNGG 3 cut(s) 109, 235, 706
BsmAI GTCTC 3 cut(s) 147, 212, 728
BsmFI GGGAC 2 cut(s) 343, 405
BsmI GAATGC 1 cut(s) 866
BsnI GGCC 2 cut(s) 712, 830
Bso31I GGTCTC 1 cut(s) 212
BsoBI CYCGRG 1 cut(s) 518
Bsp1286I GDGCHC 1 cut(s) 939
Bsp1407I TGTACA 1 cut(s) 546
Bsp143I GATC 6 cut(s) 3, 470, 571, 696, 850, 1144
BspANI GGCC 2 cut(s) 712, 830
BspCNI CTCAG 3 cut(s) 263, 662, 855
BspLI GGNNCC 4 cut(s) 331, 698, 711, 915
BspMAI CTGCAG 1 cut(s) 450
BspPI GGATC 4 cut(s) 691, 704, 858, 1139
BspTNI GGTCTC 1 cut(s) 212
BsrFI RCCGGY 1 cut(s) 235
BsrGI TGTACA 1 cut(s) 546
BsrI ACTGG 3 cut(s) 175, 1076, 1135
BssAI RCCGGY 1 cut(s) 235
BssECI CCNNGG 7 cut(s) 108, 324, 325, 519, 640, 858, 1092
BssMI GATC 6 cut(s) 3, 470, 571, 696, 850, 1144
BssT1I CCWWGG 2 cut(s) 108, 858
Bst2UI CCWGG 4 cut(s) 317, 326, 642, 815
Bst4CI ACNGT 4 cut(s) 97, 986, 1015, 1178
BstAPI GCANNNNNTGC 2 cut(s) 356, 454
BstAUI TGTACA 1 cut(s) 546
BstC8I GCNNGC 3 cut(s) 772, 828, 1171
BstDEI CTNAG 6 cut(s) 250, 282, 290, 305, 649, 842
BstF5I GGATG 3 cut(s) 185, 1001, 1081
BstHHI GCGC 1 cut(s) 311
BstKTI GATC 6 cut(s) 6, 473, 574, 699, 853, 1147
BstMAI GTCTC 3 cut(s) 147, 212, 728
BstMBI GATC 6 cut(s) 3, 470, 571, 696, 850, 1144
BstMCI CGRYCG 1 cut(s) 240
BstMWI GCNNNNNNNGC 5 cut(s) 41, 356, 365, 454, 776
BstNI CCWGG 4 cut(s) 317, 326, 642, 815
BstNSI RCATGY 1 cut(s) 1173
BstSCI CCNGG 4 cut(s) 315, 324, 640, 813
BstSFI CTRYAG 3 cut(s) 88, 446, 876
BstV1I GCAGC 6 cut(s) 194, 499, 764, 850, 1087, 1109
BstV2I GAAGAC 2 cut(s) 126, 265
BstX2I RGATCY 3 cut(s) 696, 850, 1144
BstXI CCANNNNNNTGG 1 cut(s) 926
BstYI RGATCY 3 cut(s) 696, 850, 1144
BsuRI GGCC 2 cut(s) 712, 830
BtsCI GGATG 3 cut(s) 185, 1001, 1081
BtsIMutI CAGTG 1 cut(s) 622
Cac8I GCNNGC 3 cut(s) 772, 828, 1171
CaiI CAGNNNCTG 4 cut(s) 170, 454, 755, 820
CfoI GCGC 1 cut(s) 311
Cfr10I RCCGGY 1 cut(s) 235
Cfr13I GGNCC 2 cut(s) 330, 710
Csp6I GTAC 5 cut(s) 93, 529, 547, 626, 898
CviAII CATG 3 cut(s) 344, 1044, 1170
CviQI GTAC 5 cut(s) 93, 529, 547, 626, 898
DdeI CTNAG 6 cut(s) 250, 282, 290, 305, 649, 842
DpnI GATC 6 cut(s) 5, 472, 573, 698, 852, 1146
DpnII GATC 6 cut(s) 3, 470, 571, 696, 850, 1144
DriI GACNNNNNGTC 1 cut(s) 266
Eam1105I GACNNNNNGTC 1 cut(s) 266
Ecl136II GAGCTC 1 cut(s) 937
Eco130I CCWWGG 2 cut(s) 108, 858
Eco24I GRGCYC 1 cut(s) 939
Eco31I GGTCTC 1 cut(s) 212
Eco47I GGWCC 1 cut(s) 330
Eco53kI GAGCTC 1 cut(s) 937
Eco57I CTGAAG 1 cut(s) 842
Eco88I CYCGRG 1 cut(s) 518
EcoICRI GAGCTC 1 cut(s) 937
EcoRII CCWGG 4 cut(s) 315, 324, 640, 813
EcoT14I CCWWGG 2 cut(s) 108, 858
EcoT38I GRGCYC 1 cut(s) 939
ErhI CCWWGG 2 cut(s) 108, 858
FaeI CATG 3 cut(s) 347, 1047, 1173
FalI AAGNNNNNCTT 2 cut(s) 283, 315
FaqI GGGAC 2 cut(s) 343, 405
FatI CATG 3 cut(s) 343, 1043, 1169
FauNDI CATATG 1 cut(s) 231
Fnu4HI GCNGC 6 cut(s) 183, 488, 753, 864, 1098, 1101
FokI GGATG 3 cut(s) 172, 1008, 1088
FriOI GRGCYC 1 cut(s) 939
Fsp4HI GCNGC 6 cut(s) 183, 488, 753, 864, 1098, 1101
FspBI CTAG 3 cut(s) 36, 404, 1062
GlaI GCGC 1 cut(s) 310
GluI GCNGC 6 cut(s) 183, 488, 753, 864, 1098, 1101
GsaI CCCAGC 1 cut(s) 752
GsuI CTGGAG 1 cut(s) 1152
HaeIII GGCC 2 cut(s) 712, 830
HapII CCGG 1 cut(s) 236
HhaI GCGC 1 cut(s) 311
Hin1II CATG 3 cut(s) 347, 1047, 1173
Hin6I GCGC 1 cut(s) 309
HinP1I GCGC 1 cut(s) 309
HincII GTYRAC 1 cut(s) 803
HindII GTYRAC 1 cut(s) 803
HindIII AAGCTT 1 cut(s) 540
HinfI GANTC 8 cut(s) 155, 246, 261, 286, 320, 381, 744, 1081
HpaII CCGG 1 cut(s) 236
Hpy166II GTNNAC 2 cut(s) 549, 803
Hpy188I TCNGA 7 cut(s) 152, 166, 242, 253, 380, 567, 1144
Hpy188III TCNNGA 2 cut(s) 468, 844
Hpy8I GTNNAC 2 cut(s) 549, 803
HpyAV CCTTC 3 cut(s) 199, 433, 841
HpyCH4III ACNGT 4 cut(s) 97, 986, 1015, 1178
HpyCH4IV ACGT 1 cut(s) 732
HpyCH4V TGCA 9 cut(s) 350, 448, 457, 506, 620, 779, 866, 992, 1103
HpyF10VI GCNNNNNNNGC 5 cut(s) 41, 356, 365, 454, 776
HpyF3I CTNAG 6 cut(s) 250, 282, 290, 305, 649, 842
HpySE526I ACGT 1 cut(s) 732
Hsp92II CATG 3 cut(s) 347, 1047, 1173
HspAI GCGC 1 cut(s) 309
Kzo9I GATC 6 cut(s) 3, 470, 571, 696, 850, 1144
LmnI GCTCC 4 cut(s) 65, 817, 942, 1133
Lsp1109I GCAGC 6 cut(s) 194, 499, 764, 850, 1087, 1109
LweI GCATC 2 cut(s) 194, 629
MaeI CTAG 3 cut(s) 36, 404, 1062
MaeII ACGT 1 cut(s) 732
MaeIII GTNAC 1 cut(s) 593
MalI GATC 6 cut(s) 5, 472, 573, 698, 852, 1146
MboI GATC 6 cut(s) 3, 470, 571, 696, 850, 1144
MboII GAAGA 5 cut(s) 131, 133, 255, 270, 845
MflI RGATCY 3 cut(s) 696, 850, 1144
MhlI GDGCHC 1 cut(s) 939
MluCI AATT 9 cut(s) 81, 160, 334, 352, 585, 976, 1008, 1106, 1204
MlyI GAGTC 4 cut(s) 149, 255, 295, 375
MmeI TCCRAC 1 cut(s) 220
MnlI CCTC 8 cut(s) 287, 485, 514, 517, 574, 760, 1066, 1087
MseI TTAA 4 cut(s) 512, 660, 810, 1207
MspA1I CMGCKG 2 cut(s) 755, 1100
MspI CCGG 1 cut(s) 236
MspR9I CCNGG 4 cut(s) 317, 326, 642, 815
Mva1269I GAATGC 1 cut(s) 866
MvaI CCWGG 4 cut(s) 317, 326, 642, 815
MwoI GCNNNNNNNGC 5 cut(s) 41, 356, 365, 454, 776
NdeI CATATG 1 cut(s) 231
NdeII GATC 6 cut(s) 3, 470, 571, 696, 850, 1144
NlaIII CATG 3 cut(s) 347, 1047, 1173
NlaIV GGNNCC 4 cut(s) 331, 698, 711, 915
NspI RCATGY 1 cut(s) 1173
PaeI GCATGC 1 cut(s) 1173
PasI CCCWGGG 1 cut(s) 325
PctI GAATGC 1 cut(s) 866
PfeI GAWTC 4 cut(s) 246, 320, 744, 1081
PkrI GCNGC 6 cut(s) 184, 489, 754, 865, 1099, 1102
PleI GAGTC 4 cut(s) 149, 255, 294, 375
PpsI GAGTC 4 cut(s) 149, 255, 294, 375
PsiI TTATAA 1 cut(s) 981
Psp124BI GAGCTC 1 cut(s) 939
Psp6I CCWGG 4 cut(s) 315, 324, 640, 813
PspFI CCCAGC 1 cut(s) 748
PspGI CCWGG 4 cut(s) 315, 324, 640, 813
PspN4I GGNNCC 4 cut(s) 331, 698, 711, 915
PspPI GGNCC 2 cut(s) 330, 710
PsrI GAACNNNNNNTAC 2 cut(s) 994, 1026
PstI CTGCAG 1 cut(s) 450
PstNI CAGNNNCTG 4 cut(s) 170, 454, 755, 820
PsuI RGATCY 3 cut(s) 696, 850, 1144
PvuII CAGCTG 2 cut(s) 755, 1100
RsaI GTAC 5 cut(s) 94, 530, 548, 627, 899
RsaNI GTAC 5 cut(s) 93, 529, 547, 626, 898
SacI GAGCTC 1 cut(s) 939
SaqAI TTAA 4 cut(s) 512, 660, 810, 1207
SatI GCNGC 6 cut(s) 183, 488, 753, 864, 1098, 1101
Sau3AI GATC 6 cut(s) 3, 470, 571, 696, 850, 1144
Sau96I GGNCC 2 cut(s) 330, 710
ScaI AGTACT 3 cut(s) 94, 530, 899
SchI GAGTC 4 cut(s) 149, 255, 295, 375
ScrFI CCNGG 4 cut(s) 317, 326, 642, 815
SduI GDGCHC 1 cut(s) 939
SfaNI GCATC 2 cut(s) 194, 629
SfcI CTRYAG 3 cut(s) 88, 446, 876
SinI GGWCC 1 cut(s) 330
SphI GCATGC 1 cut(s) 1173
Sse9I AATT 9 cut(s) 81, 160, 334, 352, 585, 976, 1008, 1106, 1204
SspI AATATT 1 cut(s) 658
SspMI CTAG 3 cut(s) 36, 404, 1062
SstI GAGCTC 1 cut(s) 939
StyD4I CCNGG 4 cut(s) 315, 324, 640, 813
StyI CCWWGG 2 cut(s) 108, 858
TaaI ACNGT 4 cut(s) 97, 986, 1015, 1178
TaiI ACGT 1 cut(s) 735
TaqI TCGA 3 cut(s) 270, 555, 1183
TasI AATT 9 cut(s) 81, 160, 334, 352, 585, 976, 1008, 1106, 1204
TatI WGTACW 4 cut(s) 92, 528, 546, 897
TfiI GAWTC 4 cut(s) 246, 320, 744, 1081
Tru1I TTAA 4 cut(s) 512, 660, 810, 1207
Tru9I TTAA 4 cut(s) 512, 660, 810, 1207
TscAI CASTG 1 cut(s) 622
TseI GCWGC 6 cut(s) 182, 487, 752, 863, 1097, 1100
TspDTI ATGAA 1 cut(s) 1032
TspRI CASTG 1 cut(s) 622
VpaK11BI GGWCC 1 cut(s) 330
XapI RAATTY 4 cut(s) 160, 352, 585, 1106
XceI RCATGY 1 cut(s) 1173
XcmI CCANNNNNNNNNTGG 2 cut(s) 16, 1034
XspI CTAG 3 cut(s) 36, 404, 1062
ZrmI AGTACT 3 cut(s) 94, 530, 899
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.