RLG00000011769

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000978
Physical Location & Seq
Forward (+)
12474100 .. 12476430
2331 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011769

Sequence Viewer

Length: 1161 bp
ATGGGGTACTCTCCTAAAGTTATCATTTCTCTGTTCTGCTCCTATATGCTTATATTGCATATTACCAGTAGTGCCGTGATCCCTACCTTGGCTTTGAAAGACTGTGAGTTTCCTGCCATCTTCAACTTCGGAGACTCAAATTCAGATACTGGTGGACTAGCCGCATCCCTTCTACCACCAACCACACCTTATGGAGAGACCTATTTCCATATGCCGGCCGGAAGATTCTCTGATGGAAGACTCATCATCGATTTCTTTGCAAAGAGTCTTGGTTACCCTTATCTAAGCGCATATCTGGATTCTCTCGGGACAAATTTCTCGCATGGTGCAAATTTCGCCACTGCAGCTTCCACTATCAGACTCCCAGATCTCATTATACCAGCCGGTGGATTTAGTCCTTTCTACCTTGATATTCAATATGTGCAGTTCCTGCAACTAAAATCCAGATCACAACTCATAAGGCATCAAGGGGGAATATTTGCAAGTTTAATGCCCAAGGAGGAGTACTTCTCCAAAGCTTTATACACATTCGACATTGGTCAGAATGATCTTGGCGAGGGATTTTTCGGTAACAAGACTATACAGGAAGTCAATGCATCTGTTCCTGATATAATCACTGGTTTCTCAACGAATATCAAGAAAATATATGATTTGGGAGCAAGATCATTATGGATCCACAATACGGGGCCGATTGGCTGTCTCCCTTACATTGTGGCAAACTTCCCGTCAGCTGAGAAAGATGAGATTGGCTGTGCAAAGGCTTACAATGAAGTAGCTCAACATTTTAACCACGAGCTGAAGCAGGCCATAGTTCAACTCAGGAAGGATCTTCCTTTGGCTGCATTCACTTATGTAGATGTGTATTCTGTCAAATACTCTCTTTTCAAGGAACCCGAAAAGTATGGATTTGAGCTCCCACTTGTTGCTTGTTGTGGCTCCGGTGGTGAGTACAACTATAACGCTGGATGTGGATCAATAATTACAGTCAATGGAAGCCAAATCCTTGTTGGTTCATGCAAAAACCCCTCAATTAGAGTGAACTGGGATGGTATTCATTACACCGAGGCAGCTGCGAAATTTGTTTTTGATAAAATTTCAACTGGAGCGTATTCAGATCCACCTCTTCCCTTGAAACAAGCATGTCACAGGAGTTTGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005488 GO:0006950 GO:0006952 GO:0008150 GO:0016787 GO:0016788 GO:0019863 GO:0019865 GO:0044877 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

387

Amino Acids

42.31

Weight (kDa)

6.14

Isoelectric Point (pI)

38.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 40 - 365 3.2e-47 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 386
AciI CCGC 1 cut(s) 162
AclWI GGATC 6 cut(s) 73, 667, 680, 834, 979, 1109
AcoI YGGCCR 1 cut(s) 216
AcsI RAATTY 5 cut(s) 139, 313, 331, 1076, 1092
AcuI CTGAAG 1 cut(s) 818
AfaI GTAC 3 cut(s) 8, 506, 950
AfiI CCNNNNNNNGG 4 cut(s) 88, 214, 386, 682
AgsI TTSAA 8 cut(s) 97, 124, 416, 815, 886, 1098, 1132, 1156
AluBI AGCT 7 cut(s) 347, 518, 731, 776, 796, 913, 1070
AluI AGCT 7 cut(s) 347, 518, 731, 776, 796, 913, 1070
Alw21I GWGCWC 1 cut(s) 915
Alw26I GTCTC 3 cut(s) 126, 191, 704
AlwI GGATC 6 cut(s) 73, 667, 680, 834, 979, 1109
AlwNI CAGNNNCTG 2 cut(s) 149, 430
Ama87I CYCGRG 1 cut(s) 305
AoxI GGCC 3 cut(s) 216, 686, 804
ApeKI GCWGC 4 cut(s) 344, 839, 1067, 1070
ApoI RAATTY 5 cut(s) 139, 313, 331, 1076, 1092
ArsI GACNNNNNNTTYG 2 cut(s) 710, 742
AspLEI GCGC 1 cut(s) 290
AspS9I GGNCC 1 cut(s) 686
AsuHPI GGTGA 1 cut(s) 956
AvaI CYCGRG 1 cut(s) 305
BamHI GGATCC 1 cut(s) 672
BanII GRGCYC 1 cut(s) 915
BauI CACGAG 1 cut(s) 791
BbsI GAAGAC 1 cut(s) 244
Bbv12I GWGCWC 1 cut(s) 915
BbvI GCAGC 4 cut(s) 356, 826, 1057, 1079
BccI CCATC 3 cut(s) 125, 227, 1040
BceAI ACGGC 1 cut(s) 59
BcgI CGANNNNNNTGC 4 cut(s) 239, 273, 1052, 1086
BcoDI GTCTC 3 cut(s) 126, 191, 704
BfaI CTAG 1 cut(s) 158
BfmI CTRYAG 1 cut(s) 342
BglII AGATCT 1 cut(s) 367
BisI GCNGC 5 cut(s) 162, 345, 840, 1068, 1071
BlsI GCNGC 5 cut(s) 163, 346, 841, 1069, 1072
BmcAI AGTACT 1 cut(s) 506
BmeT110I CYCGRG 1 cut(s) 305
BmgT120I GGNCC 1 cut(s) 686
BmiI GGNNCC 4 cut(s) 674, 687, 891, 937
BmrI ACTGGG 1 cut(s) 1051
BmsI GCATC 3 cut(s) 173, 472, 605
BmuI ACTGGG 1 cut(s) 1051
BoxI GACNNNNGTC 1 cut(s) 537
BpiI GAAGAC 1 cut(s) 244
BplI GAGNNNNNCTC 2 cut(s) 494, 526
BpmI CTGGAG 1 cut(s) 1122
Bsa29I ATCGAT 1 cut(s) 249
BsaBI GATNNNNATC 1 cut(s) 970
BsaI GGTCTC 1 cut(s) 191
BsaJI CCNNGG 3 cut(s) 87, 495, 1062
BsaWI WCCGGW 1 cut(s) 938
Bsc4I CCNNNNNNNGG 4 cut(s) 88, 214, 386, 682
Bse118I RCCGGY 2 cut(s) 214, 383
Bse1I ACTGG 5 cut(s) 66, 154, 622, 1046, 1105
Bse8I GATNNNNATC 1 cut(s) 970
BseCI ATCGAT 1 cut(s) 249
BseDI CCNNGG 3 cut(s) 87, 495, 1062
BseGI GGATG 3 cut(s) 164, 971, 1051
BseJI GATNNNNATC 1 cut(s) 970
BseLI CCNNNNNNNGG 4 cut(s) 88, 214, 386, 682
BseMII CTCAG 2 cut(s) 723, 832
BseNI ACTGG 5 cut(s) 66, 154, 622, 1046, 1105
BseRI GAGGAG 1 cut(s) 515
BseX3I CGGCCG 1 cut(s) 216
BseXI GCAGC 4 cut(s) 356, 826, 1057, 1079
BsgI GTGCAG 1 cut(s) 443
Bsh1285I CGRYCG 1 cut(s) 219
BshFI GGCC 3 cut(s) 218, 688, 806
BshVI ATCGAT 1 cut(s) 249
BsiEI CGRYCG 1 cut(s) 219
BsiHKAI GWGCWC 1 cut(s) 915
BsiHKCI CYCGRG 1 cut(s) 305
BsiSI CCGG 4 cut(s) 215, 219, 384, 939
BslFI GGGAC 1 cut(s) 322
BslI CCNNNNNNNGG 4 cut(s) 88, 214, 386, 682
BsmAI GTCTC 3 cut(s) 126, 191, 704
BsmFI GGGAC 1 cut(s) 322
BsmI GAATGC 1 cut(s) 842
BsnI GGCC 3 cut(s) 218, 688, 806
Bso31I GGTCTC 1 cut(s) 191
BsoBI CYCGRG 1 cut(s) 305
Bsp1286I GDGCHC 1 cut(s) 915
Bsp143I GATC 9 cut(s) 78, 367, 446, 547, 662, 672, 826, 971, 1114
BspACI CCGC 1 cut(s) 162
BspANI GGCC 3 cut(s) 218, 688, 806
BspCNI CTCAG 2 cut(s) 724, 831
BspDI ATCGAT 1 cut(s) 249
BspLI GGNNCC 4 cut(s) 674, 687, 891, 937
BspMAI CTGCAG 1 cut(s) 346
BspPI GGATC 6 cut(s) 73, 667, 680, 834, 979, 1109
BspTNI GGTCTC 1 cut(s) 191
BsrFI RCCGGY 2 cut(s) 214, 383
BsrI ACTGG 5 cut(s) 66, 154, 622, 1046, 1105
BssAI RCCGGY 2 cut(s) 214, 383
BssECI CCNNGG 3 cut(s) 87, 495, 1062
BssMI GATC 9 cut(s) 78, 367, 446, 547, 662, 672, 826, 971, 1114
BssSI CACGAG 1 cut(s) 791
BssT1I CCWWGG 2 cut(s) 87, 495
Bst2BI CACGAG 1 cut(s) 791
Bst4CI ACNGT 2 cut(s) 104, 985
Bst6I CTCTTC 1 cut(s) 1128
BstAPI GCANNNNNTGC 1 cut(s) 430
BstC8I GCNNGC 2 cut(s) 216, 804
BstDEI CTNAG 3 cut(s) 284, 732, 818
BstEII GGTNACC 1 cut(s) 272
BstF5I GGATG 3 cut(s) 164, 971, 1051
BstHHI GCGC 1 cut(s) 290
BstKTI GATC 9 cut(s) 81, 370, 449, 550, 665, 675, 829, 974, 1117
BstMAI GTCTC 3 cut(s) 126, 191, 704
BstMBI GATC 9 cut(s) 78, 367, 446, 547, 662, 672, 826, 971, 1114
BstMCI CGRYCG 1 cut(s) 219
BstMWI GCNNNNNNNGC 4 cut(s) 55, 335, 344, 430
BstNSI RCATGY 1 cut(s) 1143
BstPAI GACNNNNGTC 1 cut(s) 537
BstPI GGTNACC 1 cut(s) 272
BstSFI CTRYAG 1 cut(s) 342
BstV1I GCAGC 4 cut(s) 356, 826, 1057, 1079
BstV2I GAAGAC 1 cut(s) 244
BstX2I RGATCY 4 cut(s) 367, 672, 826, 1114
BstYI RGATCY 4 cut(s) 367, 672, 826, 1114
BstZI CGGCCG 1 cut(s) 216
Bsu15I ATCGAT 1 cut(s) 249
BsuRI GGCC 3 cut(s) 218, 688, 806
BsuTUI ATCGAT 1 cut(s) 249
BtsCI GGATG 3 cut(s) 164, 971, 1051
BtsI GCAGTG 1 cut(s) 339
BtsIMutI CAGTG 2 cut(s) 339, 615
Cac8I GCNNGC 2 cut(s) 216, 804
CaiI CAGNNNCTG 2 cut(s) 149, 430
CfoI GCGC 1 cut(s) 290
Cfr10I RCCGGY 2 cut(s) 214, 383
Cfr13I GGNCC 1 cut(s) 686
ClaI ATCGAT 1 cut(s) 249
Csp6I GTAC 3 cut(s) 7, 505, 949
CviAII CATG 3 cut(s) 323, 1014, 1140
CviQI GTAC 3 cut(s) 7, 505, 949
DdeI CTNAG 3 cut(s) 284, 732, 818
DpnI GATC 9 cut(s) 80, 369, 448, 549, 664, 674, 828, 973, 1116
DpnII GATC 9 cut(s) 78, 367, 446, 547, 662, 672, 826, 971, 1114
EaeI YGGCCR 1 cut(s) 216
EagI CGGCCG 1 cut(s) 216
Eam1104I CTCTTC 1 cut(s) 1128
EarI CTCTTC 1 cut(s) 1128
Ecl136II GAGCTC 1 cut(s) 913
EclXI CGGCCG 1 cut(s) 216
Eco130I CCWWGG 2 cut(s) 87, 495
Eco24I GRGCYC 1 cut(s) 915
Eco31I GGTCTC 1 cut(s) 191
Eco52I CGGCCG 1 cut(s) 216
Eco53kI GAGCTC 1 cut(s) 913
Eco57I CTGAAG 1 cut(s) 818
Eco88I CYCGRG 1 cut(s) 305
Eco91I GGTNACC 1 cut(s) 272
EcoICRI GAGCTC 1 cut(s) 913
EcoO65I GGTNACC 1 cut(s) 272
EcoT14I CCWWGG 2 cut(s) 87, 495
EcoT22I ATGCAT 1 cut(s) 598
EcoT38I GRGCYC 1 cut(s) 915
ErhI CCWWGG 2 cut(s) 87, 495
FaeI CATG 3 cut(s) 326, 1017, 1143
FaqI GGGAC 1 cut(s) 322
FatI CATG 3 cut(s) 322, 1013, 1139
FauNDI CATATG 1 cut(s) 210
Fnu4HI GCNGC 5 cut(s) 162, 345, 840, 1068, 1071
FokI GGATG 3 cut(s) 151, 978, 1058
FriOI GRGCYC 1 cut(s) 915
Fsp4HI GCNGC 5 cut(s) 162, 345, 840, 1068, 1071
FspBI CTAG 1 cut(s) 158
GlaI GCGC 1 cut(s) 289
GluI GCNGC 5 cut(s) 162, 345, 840, 1068, 1071
GsuI CTGGAG 1 cut(s) 1122
HaeIII GGCC 3 cut(s) 218, 688, 806
HapII CCGG 4 cut(s) 215, 219, 384, 939
HhaI GCGC 1 cut(s) 290
Hin1II CATG 3 cut(s) 326, 1017, 1143
Hin6I GCGC 1 cut(s) 288
HinP1I GCGC 1 cut(s) 288
HindIII AAGCTT 1 cut(s) 516
HinfI GANTC 6 cut(s) 134, 225, 240, 265, 299, 360
HpaII CCGG 4 cut(s) 215, 219, 384, 939
HphI GGTGA 1 cut(s) 956
Hpy166II GTNNAC 2 cut(s) 155, 1039
Hpy188I TCNGA 6 cut(s) 131, 145, 232, 359, 543, 1114
Hpy188III TCNNGA 6 cut(s) 296, 307, 444, 605, 637, 820
Hpy8I GTNNAC 2 cut(s) 155, 1039
HpyAV CCTTC 2 cut(s) 179, 817
HpyCH4III ACNGT 2 cut(s) 104, 985
HpyF10VI GCNNNNNNNGC 4 cut(s) 55, 335, 344, 430
HpyF3I CTNAG 3 cut(s) 284, 732, 818
Hsp92II CATG 3 cut(s) 326, 1017, 1143
HspAI GCGC 1 cut(s) 288
KroI GCCGGC 1 cut(s) 214
KroNI GCCGGC 1 cut(s) 216
Kzo9I GATC 9 cut(s) 78, 367, 446, 547, 662, 672, 826, 971, 1114
LmnI GCTCC 5 cut(s) 44, 656, 918, 941, 1103
Lsp1109I GCAGC 4 cut(s) 356, 826, 1057, 1079
LweI GCATC 3 cut(s) 173, 472, 605
MaeI CTAG 1 cut(s) 158
MaeIII GTNAC 3 cut(s) 272, 569, 1142
MalI GATC 9 cut(s) 80, 369, 448, 549, 664, 674, 828, 973, 1116
MboI GATC 9 cut(s) 78, 367, 446, 547, 662, 672, 826, 971, 1114
MboII GAAGA 5 cut(s) 112, 234, 249, 821, 1115
MflI RGATCY 4 cut(s) 367, 672, 826, 1114
MhlI GDGCHC 1 cut(s) 915
MluCI AATT 7 cut(s) 139, 313, 331, 978, 1029, 1076, 1092
MlyI GAGTC 4 cut(s) 128, 234, 274, 354
MnlI CCTC 5 cut(s) 493, 550, 1036, 1057, 1131
Mph1103I ATGCAT 1 cut(s) 598
MroNI GCCGGC 1 cut(s) 214
MseI TTAA 2 cut(s) 488, 786
MspA1I CMGCKG 2 cut(s) 731, 1070
MspI CCGG 4 cut(s) 215, 219, 384, 939
Mva1269I GAATGC 1 cut(s) 842
MwoI GCNNNNNNNGC 4 cut(s) 55, 335, 344, 430
NaeI GCCGGC 1 cut(s) 216
NdeI CATATG 1 cut(s) 210
NdeII GATC 9 cut(s) 78, 367, 446, 547, 662, 672, 826, 971, 1114
NgoMIV GCCGGC 1 cut(s) 214
NlaIII CATG 3 cut(s) 326, 1017, 1143
NlaIV GGNNCC 4 cut(s) 674, 687, 891, 937
NmuCI GTSAC 1 cut(s) 1142
NsiI ATGCAT 1 cut(s) 598
NspI RCATGY 1 cut(s) 1143
PctI GAATGC 1 cut(s) 842
PdiI GCCGGC 1 cut(s) 216
PfeI GAWTC 2 cut(s) 225, 299
PflMI CCANNNNNTGG 1 cut(s) 386
PkrI GCNGC 5 cut(s) 163, 346, 841, 1069, 1072
PleI GAGTC 4 cut(s) 128, 234, 273, 354
PpsI GAGTC 4 cut(s) 128, 234, 273, 354
PshAI GACNNNNGTC 1 cut(s) 537
Psp124BI GAGCTC 1 cut(s) 915
PspEI GGTNACC 1 cut(s) 272
PspN4I GGNNCC 4 cut(s) 674, 687, 891, 937
PspPI GGNCC 1 cut(s) 686
PstI CTGCAG 1 cut(s) 346
PstNI CAGNNNCTG 2 cut(s) 149, 430
PsuI RGATCY 4 cut(s) 367, 672, 826, 1114
PvuII CAGCTG 2 cut(s) 731, 1070
RsaI GTAC 3 cut(s) 8, 506, 950
RsaNI GTAC 3 cut(s) 7, 505, 949
SacI GAGCTC 1 cut(s) 915
SaqAI TTAA 2 cut(s) 488, 786
SatI GCNGC 5 cut(s) 162, 345, 840, 1068, 1071
Sau3AI GATC 9 cut(s) 78, 367, 446, 547, 662, 672, 826, 971, 1114
Sau96I GGNCC 1 cut(s) 686
ScaI AGTACT 1 cut(s) 506
SchI GAGTC 4 cut(s) 128, 234, 274, 354
SduI GDGCHC 1 cut(s) 915
SfaNI GCATC 3 cut(s) 173, 472, 605
SfcI CTRYAG 1 cut(s) 342
Sse9I AATT 7 cut(s) 139, 313, 331, 978, 1029, 1076, 1092
SsiI CCGC 1 cut(s) 162
SspI AATATT 1 cut(s) 477
SspMI CTAG 1 cut(s) 158
SstI GAGCTC 1 cut(s) 915
StyI CCWWGG 2 cut(s) 87, 495
TaaI ACNGT 2 cut(s) 104, 985
TaqI TCGA 2 cut(s) 249, 531
TasI AATT 7 cut(s) 139, 313, 331, 978, 1029, 1076, 1092
TatI WGTACW 2 cut(s) 504, 948
TauI GCSGC 1 cut(s) 164
TfiI GAWTC 2 cut(s) 225, 299
Tru1I TTAA 2 cut(s) 488, 786
Tru9I TTAA 2 cut(s) 488, 786
TscAI CASTG 2 cut(s) 346, 622
TseFI GTSAC 1 cut(s) 1142
TseI GCWGC 4 cut(s) 344, 839, 1067, 1070
Tsp45I GTSAC 1 cut(s) 1142
TspDTI ATGAA 3 cut(s) 783, 1002, 1043
TspRI CASTG 2 cut(s) 346, 622
Van91I CCANNNNNTGG 1 cut(s) 386
XapI RAATTY 5 cut(s) 139, 313, 331, 1076, 1092
XceI RCATGY 1 cut(s) 1143
XcmI CCANNNNNNNNNTGG 1 cut(s) 1004
XspI CTAG 1 cut(s) 158
ZrmI AGTACT 1 cut(s) 506
Zsp2I ATGCAT 1 cut(s) 598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.