Prupe.1G497400_v2.0.a1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000978
Physical Location & Seq
Forward (+)
41198565 .. 41205204
6640 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G497400.2

Sequence Viewer

Length: 1278 bp
ATGCAGTTGAAAATTCCACCTTTTCCTTGCACTATAAAAGGAACATACTCTTGGATAATTTATCACTTCTTATCTGCAACATCTTTGTGGTATATATTTGCTTTCTCAACCATGGACTCTCCTACATTTCCTATCCAAGCTATCGTTTCTCTATTTTGCTCATGTATGCTTTTGTCGCATATCACCACTTTGAGCCCTGCCTTGGCTTTGGAAGACTGTGAGTTTCCAGCCATCTTTAACTTTGGAGATTCAAATTCAGATACTGGTGGATTTGCCGCGTCCCTTTCGCCACCAACACCACCTTATGGGGAGACCTATTTTCATATGCCGGTTGGAAGATTCTCAGATGGCAGGCTCATAATCGATTTTCTTGCAAAGAGTCTTGGTCACCCCTTTCTAAGCGCATATCTGGATTCCCTTGGGAGCAACTTCTCGTATGGTGCAAATTTTGCCACTGCAGCTTCCACCATCAGATTGCCAGATCAAATTTTTCCGGCTGGTGGATTTAGTCCCTTCTACCTCAATATTCAGTACGTGCAGTTCATGCAACTAAAATCCAGATCACAACTCATTAGGCATCGAGGGGGAATATTTGCAAGTTTGATGCCCAAGGAGGAGTATTTCTCCAAAGCTTTATACACATTTGACATTGGACAGAATGACCTAGGTGAGGGGTTCTCTGGTAACTTGACTGTTCAAGAGGTCAATGCATCGGTTCCTGATATAATCAGTGGGTTCTCGGCAAATATTAAGAAAATATATGATTTGGGAGCGAGATCATTTTGGATCCACAACACTGGGCCAATTGGCTGTCTCCCTTACATCTTTGCCAATTTCCCAGCTCAAAAGGATGAGGCCGGCTGTGCAAAGTCATACAATGAAGTAGCTCAGCATTTTAACCAGAAGCTGAAGGAAGCCACAGTTCAACTTAGGAAAGATCTACCTTTGGCTGCAATTACTTATGTGGACGTTTATTCAGTTAAATACTCTCTTTTCAAGGAATCGCAAAAATATGGGTTTGAGCTCCCACTTGTTGCTTGTTGTGGGTATGGTGGCAAGTACAACTATAACAGTAGTTCTGAATGTGGAGGAACAGCTAGAGTTAATGGAAGGCAAATATTTGTTGGCTCATGCAAAGATCCTTCGGTGAGAGTGAATTGGGATGGCATTCATTATACTGAGGCAGCTGCCAAGTTTATTTCTGATAAAATTTCAACTGGAGCATTTTCTGATCCACCCCTTGCTTTGAAACAAGCATGTCACAAGAATTTGGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005488 GO:0006950 GO:0006952 GO:0008150 GO:0016787 GO:0016788 GO:0019863 GO:0019865 GO:0044877 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

426

Amino Acids

46.81

Weight (kDa)

7.07

Isoelectric Point (pI)

41.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 305
AccII CGCG 1 cut(s) 278
AciI CCGC 1 cut(s) 276
AclWI GGATC 4 cut(s) 781, 794, 1133, 1226
AcsI RAATTY 6 cut(s) 12, 253, 445, 486, 1209, 1267
AcuI CTGAAG 1 cut(s) 929
AfaI GTAC 2 cut(s) 533, 1061
AfiI CCNNNNNNNGG 4 cut(s) 202, 305, 500, 670
AgsI TTSAA 7 cut(s) 10, 252, 698, 926, 997, 1215, 1249
AjuI GAANNNNNNNTTGG 2 cut(s) 34, 66
AluBI AGCT 9 cut(s) 140, 461, 632, 842, 887, 907, 1024, 1097, 1187
AluI AGCT 9 cut(s) 140, 461, 632, 842, 887, 907, 1024, 1097, 1187
Alw21I GWGCWC 1 cut(s) 1026
Alw26I GTCTC 2 cut(s) 305, 818
AlwI GGATC 4 cut(s) 781, 794, 1133, 1226
AlwNI CAGNNNCTG 2 cut(s) 263, 907
AoxI GGCC 3 cut(s) 800, 855, 1272
ApeKI GCWGC 4 cut(s) 458, 950, 1184, 1187
ApoI RAATTY 6 cut(s) 12, 253, 445, 486, 1209, 1267
AspA2I CCTAGG 1 cut(s) 664
AspLEI GCGC 1 cut(s) 404
AspS9I GGNCC 1 cut(s) 800
AsuHPI GGTGA 4 cut(s) 175, 380, 680, 1159
AvrII CCTAGG 1 cut(s) 664
BamHI GGATCC 1 cut(s) 786
BanII GRGCYC 2 cut(s) 197, 1026
BbsI GAAGAC 1 cut(s) 219
Bbv12I GWGCWC 1 cut(s) 1026
BbvI GCAGC 4 cut(s) 470, 937, 1174, 1196
BccI CCATC 4 cut(s) 239, 341, 476, 1157
BcgI CGANNNNNNTGC 2 cut(s) 353, 387
BcoDI GTCTC 2 cut(s) 305, 818
BfaI CTAG 2 cut(s) 665, 1098
BfmI CTRYAG 1 cut(s) 456
BglII AGATCT 1 cut(s) 937
BisI GCNGC 5 cut(s) 276, 459, 951, 1185, 1188
BlnI CCTAGG 1 cut(s) 664
BlpI GCTNAGC 1 cut(s) 888
BlsI GCNGC 5 cut(s) 277, 460, 952, 1186, 1189
BmgT120I GGNCC 1 cut(s) 800
BmiI GGNNCC 2 cut(s) 717, 788
BmrI ACTGGG 1 cut(s) 807
BmsI GCATC 3 cut(s) 586, 594, 719
BmuI ACTGGG 1 cut(s) 807
BpiI GAAGAC 1 cut(s) 219
BplI GAGNNNNNCTC 4 cut(s) 608, 640, 662, 694
BpmI CTGGAG 1 cut(s) 1239
Bpu1102I GCTNAGC 1 cut(s) 888
Bsa29I ATCGAT 1 cut(s) 363
BsaAI YACGTR 1 cut(s) 535
BsaI GGTCTC 1 cut(s) 305
BsaJI CCNNGG 5 cut(s) 111, 201, 418, 609, 664
Bsc4I CCNNNNNNNGG 4 cut(s) 202, 305, 500, 670
Bse118I RCCGGY 2 cut(s) 328, 857
Bse1I ACTGG 3 cut(s) 268, 802, 1222
BseCI ATCGAT 1 cut(s) 363
BseDI CCNNGG 5 cut(s) 111, 201, 418, 609, 664
BseGI GGATG 2 cut(s) 856, 1168
BseLI CCNNNNNNNGG 4 cut(s) 202, 305, 500, 670
BseMII CTCAG 3 cut(s) 357, 902, 1170
BseNI ACTGG 3 cut(s) 268, 802, 1222
BseRI GAGGAG 1 cut(s) 629
BseXI GCAGC 4 cut(s) 470, 937, 1174, 1196
BseYI CCCAGC 1 cut(s) 838
BsgI GTGCAG 1 cut(s) 557
Bsh1236I CGCG 1 cut(s) 278
BshFI GGCC 3 cut(s) 802, 857, 1274
BshVI ATCGAT 1 cut(s) 363
BsiHKAI GWGCWC 1 cut(s) 1026
BsiSI CCGG 3 cut(s) 329, 494, 858
BslFI GGGAC 2 cut(s) 265, 495
BslI CCNNNNNNNGG 4 cut(s) 202, 305, 500, 670
BsmAI GTCTC 2 cut(s) 305, 818
BsmFI GGGAC 2 cut(s) 265, 495
BsmI GAATGC 1 cut(s) 1167
BsnI GGCC 3 cut(s) 802, 857, 1274
Bso31I GGTCTC 1 cut(s) 305
Bsp1286I GDGCHC 2 cut(s) 197, 1026
Bsp143I GATC 7 cut(s) 481, 560, 776, 786, 937, 1138, 1231
Bsp1720I GCTNAGC 1 cut(s) 888
Bsp19I CCATGG 1 cut(s) 111
BspACI CCGC 1 cut(s) 276
BspANI GGCC 3 cut(s) 802, 857, 1274
BspCNI CTCAG 3 cut(s) 356, 901, 1171
BspDI ATCGAT 1 cut(s) 363
BspFNI CGCG 1 cut(s) 278
BspLI GGNNCC 2 cut(s) 717, 788
BspMAI CTGCAG 1 cut(s) 460
BspPI GGATC 4 cut(s) 781, 794, 1133, 1226
BspTNI GGTCTC 1 cut(s) 305
BsrFI RCCGGY 2 cut(s) 328, 857
BsrI ACTGG 3 cut(s) 268, 802, 1222
BssAI RCCGGY 2 cut(s) 328, 857
BssECI CCNNGG 5 cut(s) 111, 201, 418, 609, 664
BssMI GATC 7 cut(s) 481, 560, 776, 786, 937, 1138, 1231
BssT1I CCWWGG 5 cut(s) 111, 201, 418, 609, 664
Bst4CI ACNGT 4 cut(s) 218, 694, 922, 1073
BstAPI GCANNNNNTGC 2 cut(s) 449, 544
BstBAI YACGTR 1 cut(s) 535
BstC8I GCNNGC 2 cut(s) 353, 859
BstDEI CTNAG 5 cut(s) 343, 398, 888, 929, 1179
BstDSI CCRYGG 1 cut(s) 111
BstEII GGTNACC 1 cut(s) 386
BstENI CCTNNNNNAGG 1 cut(s) 668
BstF5I GGATG 2 cut(s) 856, 1168
BstFNI CGCG 1 cut(s) 278
BstHHI GCGC 1 cut(s) 404
BstKTI GATC 7 cut(s) 484, 563, 779, 789, 940, 1141, 1234
BstMAI GTCTC 2 cut(s) 305, 818
BstMBI GATC 7 cut(s) 481, 560, 776, 786, 937, 1138, 1231
BstMWI GCNNNNNNNGC 5 cut(s) 175, 449, 458, 544, 863
BstNSI RCATGY 1 cut(s) 1260
BstPI GGTNACC 1 cut(s) 386
BstSFI CTRYAG 1 cut(s) 456
BstUI CGCG 1 cut(s) 278
BstV1I GCAGC 4 cut(s) 470, 937, 1174, 1196
BstV2I GAAGAC 1 cut(s) 219
BstX2I RGATCY 3 cut(s) 786, 937, 1138
BstXI CCANNNNNNTGG 1 cut(s) 797
BstYI RGATCY 3 cut(s) 786, 937, 1138
Bsu15I ATCGAT 1 cut(s) 363
BsuRI GGCC 3 cut(s) 802, 857, 1274
BsuTUI ATCGAT 1 cut(s) 363
BtgI CCRYGG 1 cut(s) 111
BtsCI GGATG 2 cut(s) 856, 1168
BtsI GCAGTG 1 cut(s) 453
BtsIMutI CAGTG 3 cut(s) 453, 736, 795
Cac8I GCNNGC 2 cut(s) 353, 859
CaiI CAGNNNCTG 2 cut(s) 263, 907
CfoI GCGC 1 cut(s) 404
Cfr10I RCCGGY 2 cut(s) 328, 857
Cfr13I GGNCC 1 cut(s) 800
ClaI ATCGAT 1 cut(s) 363
CseI GACGC 1 cut(s) 267
Csp6I GTAC 2 cut(s) 532, 1060
CviAII CATG 5 cut(s) 112, 162, 544, 1131, 1257
CviQI GTAC 2 cut(s) 532, 1060
DdeI CTNAG 5 cut(s) 343, 398, 888, 929, 1179
DpnI GATC 7 cut(s) 483, 562, 778, 788, 939, 1140, 1233
DpnII GATC 7 cut(s) 481, 560, 776, 786, 937, 1138, 1231
Ecl136II GAGCTC 1 cut(s) 1024
Eco130I CCWWGG 5 cut(s) 111, 201, 418, 609, 664
Eco24I GRGCYC 2 cut(s) 197, 1026
Eco31I GGTCTC 1 cut(s) 305
Eco53kI GAGCTC 1 cut(s) 1024
Eco57I CTGAAG 1 cut(s) 929
Eco91I GGTNACC 1 cut(s) 386
EcoICRI GAGCTC 1 cut(s) 1024
EcoNI CCTNNNNNAGG 1 cut(s) 668
EcoO65I GGTNACC 1 cut(s) 386
EcoT14I CCWWGG 5 cut(s) 111, 201, 418, 609, 664
EcoT22I ATGCAT 1 cut(s) 712
EcoT38I GRGCYC 2 cut(s) 197, 1026
ErhI CCWWGG 5 cut(s) 111, 201, 418, 609, 664
FaeI CATG 5 cut(s) 115, 165, 547, 1134, 1260
FaqI GGGAC 2 cut(s) 265, 495
FatI CATG 5 cut(s) 111, 161, 543, 1130, 1256
FauNDI CATATG 1 cut(s) 324
Fnu4HI GCNGC 5 cut(s) 276, 459, 951, 1185, 1188
FokI GGATG 2 cut(s) 863, 1175
FriOI GRGCYC 2 cut(s) 197, 1026
Fsp4HI GCNGC 5 cut(s) 276, 459, 951, 1185, 1188
FspBI CTAG 2 cut(s) 665, 1098
GlaI GCGC 1 cut(s) 403
GluI GCNGC 5 cut(s) 276, 459, 951, 1185, 1188
GsaI CCCAGC 1 cut(s) 842
GsuI CTGGAG 1 cut(s) 1239
HaeIII GGCC 3 cut(s) 802, 857, 1274
HapII CCGG 3 cut(s) 329, 494, 858
HgaI GACGC 1 cut(s) 267
HhaI GCGC 1 cut(s) 404
Hin1II CATG 5 cut(s) 115, 165, 547, 1134, 1260
Hin6I GCGC 1 cut(s) 402
HinP1I GCGC 1 cut(s) 402
HindIII AAGCTT 1 cut(s) 630
HinfI GANTC 6 cut(s) 116, 248, 339, 379, 413, 1001
HpaII CCGG 3 cut(s) 329, 494, 858
HphI GGTGA 4 cut(s) 175, 380, 680, 1159
Hpy166II GTNNAC 1 cut(s) 967
Hpy188I TCNGA 6 cut(s) 259, 346, 473, 1081, 1204, 1231
Hpy188III TCNNGA 4 cut(s) 410, 558, 698, 719
Hpy8I GTNNAC 1 cut(s) 967
HpyAV CCTTC 4 cut(s) 523, 904, 1104, 1152
HpyCH4III ACNGT 4 cut(s) 218, 694, 922, 1073
HpyCH4IV ACGT 2 cut(s) 534, 969
HpyF10VI GCNNNNNNNGC 5 cut(s) 175, 449, 458, 544, 863
HpyF3I CTNAG 5 cut(s) 343, 398, 888, 929, 1179
HpySE526I ACGT 2 cut(s) 534, 969
Hsp92II CATG 5 cut(s) 115, 165, 547, 1134, 1260
HspAI GCGC 1 cut(s) 402
KroI GCCGGC 1 cut(s) 857
KroNI GCCGGC 1 cut(s) 859
Kzo9I GATC 7 cut(s) 481, 560, 776, 786, 937, 1138, 1231
LmnI GCTCC 4 cut(s) 423, 770, 1029, 1220
Lsp1109I GCAGC 4 cut(s) 470, 937, 1174, 1196
LweI GCATC 3 cut(s) 586, 594, 719
MaeI CTAG 2 cut(s) 665, 1098
MaeII ACGT 2 cut(s) 534, 969
MaeIII GTNAC 3 cut(s) 386, 683, 1259
MalI GATC 7 cut(s) 483, 562, 778, 788, 939, 1140, 1233
MboI GATC 7 cut(s) 481, 560, 776, 786, 937, 1138, 1231
MboII GAAGA 2 cut(s) 224, 348
MfeI CAATTG 1 cut(s) 804
MflI RGATCY 3 cut(s) 786, 937, 1138
MhlI GDGCHC 2 cut(s) 197, 1026
MlyI GAGTC 2 cut(s) 110, 388
MmeI TCCRAC 1 cut(s) 313
MnlI CCTC 8 cut(s) 530, 575, 607, 664, 694, 847, 1082, 1174
Mph1103I ATGCAT 1 cut(s) 712
MroNI GCCGGC 1 cut(s) 857
MseI TTAA 5 cut(s) 237, 750, 897, 981, 1104
MslI CAYNNNNRTG 1 cut(s) 85
MspA1I CMGCKG 1 cut(s) 1187
MspI CCGG 3 cut(s) 329, 494, 858
MunI CAATTG 1 cut(s) 804
Mva1269I GAATGC 1 cut(s) 1167
MvnI CGCG 1 cut(s) 278
MwoI GCNNNNNNNGC 5 cut(s) 175, 449, 458, 544, 863
NaeI GCCGGC 1 cut(s) 859
NcoI CCATGG 1 cut(s) 111
NdeI CATATG 1 cut(s) 324
NdeII GATC 7 cut(s) 481, 560, 776, 786, 937, 1138, 1231
NgoMIV GCCGGC 1 cut(s) 857
NlaIII CATG 5 cut(s) 115, 165, 547, 1134, 1260
NlaIV GGNNCC 2 cut(s) 717, 788
NmeAIII GCCGAG 1 cut(s) 719
NmuCI GTSAC 2 cut(s) 386, 1259
NsiI ATGCAT 1 cut(s) 712
NspI RCATGY 1 cut(s) 1260
PctI GAATGC 1 cut(s) 1167
PdiI GCCGGC 1 cut(s) 859
PfeI GAWTC 4 cut(s) 248, 339, 413, 1001
PflMI CCANNNNNTGG 1 cut(s) 305
PkrI GCNGC 5 cut(s) 277, 460, 952, 1186, 1189
PleI GAGTC 2 cut(s) 110, 387
PpsI GAGTC 2 cut(s) 110, 387
Ppu21I YACGTR 1 cut(s) 535
Psp124BI GAGCTC 1 cut(s) 1026
PspEI GGTNACC 1 cut(s) 386
PspFI CCCAGC 1 cut(s) 838
PspN4I GGNNCC 2 cut(s) 717, 788
PspPI GGNCC 1 cut(s) 800
PsrI GAACNNNNNNTAC 2 cut(s) 524, 556
PstI CTGCAG 1 cut(s) 460
PstNI CAGNNNCTG 2 cut(s) 263, 907
PsuI RGATCY 3 cut(s) 786, 937, 1138
PvuII CAGCTG 1 cut(s) 1187
RsaI GTAC 2 cut(s) 533, 1061
RsaNI GTAC 2 cut(s) 532, 1060
RseI CAYNNNNRTG 1 cut(s) 85
SacI GAGCTC 1 cut(s) 1026
SaqAI TTAA 5 cut(s) 237, 750, 897, 981, 1104
SatI GCNGC 5 cut(s) 276, 459, 951, 1185, 1188
Sau3AI GATC 7 cut(s) 481, 560, 776, 786, 937, 1138, 1231
Sau96I GGNCC 1 cut(s) 800
SchI GAGTC 2 cut(s) 110, 388
SduI GDGCHC 2 cut(s) 197, 1026
SfaNI GCATC 3 cut(s) 586, 594, 719
SfcI CTRYAG 1 cut(s) 456
SmiMI CAYNNNNRTG 1 cut(s) 85
SsiI CCGC 1 cut(s) 276
SspI AATATT 4 cut(s) 526, 591, 748, 1119
SspMI CTAG 2 cut(s) 665, 1098
SstI GAGCTC 1 cut(s) 1026
StyI CCWWGG 5 cut(s) 111, 201, 418, 609, 664
TaaI ACNGT 4 cut(s) 218, 694, 922, 1073
TaiI ACGT 2 cut(s) 537, 972
TaqI TCGA 2 cut(s) 363, 580
TatI WGTACW 1 cut(s) 1059
TauI GCSGC 1 cut(s) 278
TfiI GAWTC 4 cut(s) 248, 339, 413, 1001
Tru1I TTAA 5 cut(s) 237, 750, 897, 981, 1104
Tru9I TTAA 5 cut(s) 237, 750, 897, 981, 1104
TscAI CASTG 3 cut(s) 460, 736, 802
TseFI GTSAC 2 cut(s) 386, 1259
TseI GCWGC 4 cut(s) 458, 950, 1184, 1187
Tsp45I GTSAC 2 cut(s) 386, 1259
TspDTI ATGAA 4 cut(s) 311, 532, 894, 1160
TspRI CASTG 3 cut(s) 460, 736, 802
Van91I CCANNNNNTGG 1 cut(s) 305
XagI CCTNNNNNAGG 1 cut(s) 668
XapI RAATTY 6 cut(s) 12, 253, 445, 486, 1209, 1267
XceI RCATGY 1 cut(s) 1260
XmaJI CCTAGG 1 cut(s) 664
XspI CTAG 2 cut(s) 665, 1098
Zsp2I ATGCAT 1 cut(s) 712
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.