RLG00000011768

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000978
Physical Location & Seq
Forward (+)
12469554 .. 12471908
2355 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011768

Sequence Viewer

Length: 1182 bp
ATGGGGTACCCTCCTAGAGTTATTATTTCTCTGTTCTGCTCCTATATGCTTATATTGCATATTACCAGTAGTACCTTAGCTTTGAAAGACTGCGAGTTTCCAGCCATCTTCAACTTCGGAGACTCAAATTCAGATACTGGTGGACTAGCCGCATCCCTTCTACCACCAACCACACCTTATGGAGAGACCTATTTCCATATGCCGGTCGGAAGATTCTCTGATGGAAGACTCATTATCGATTTCCTGGCAAAGAGTCTTGGTCACCCTTATCTAAGCGCATATCTGGATTCTCTCGGGACAAATTTCTCGCATGGTGCAAATTTCGCCACTGCAGCTTCCACTATCAGACTCCCAGATCTCATTATACCAGCCGGTGGATTTAGTCCTTTCTACCTTGATATTCAATATGTGCAGTTCCTGCAACTAAAATCCAGATCACAACTCATAAGGCATCGAGGGGGAATATTTGCAAGTTTACTGCCCAAGAAGGATTACTTCTCCAAAGCTTTATACACATTCGACATTGGTCAGAATGATCTTGGCGAGGGATTTTTCGGTAACATGACTATACAGGAAGTTAATGCATCTGTTCCTGATATAATCACCGGTTTCTCAACGAATATCAAGAAAATATATGATTTGGGAGCAAGATCATTATGGATCCACAATACGGGGCCGATTGGCTGTCTCCCTTACATTGTGGCAAACTTCCCGTCAGCTGAGAAAGATGAGATTGGCTGTGCAAAGGCTTACAATGAAGTAGCTCAACATTTTAACCACGAGCTGAAGCACGCCATAGTTCAACTCAGGAAGGATCTTCCTAAGGCTGCATTCACTTATGTAGATGTGTATTCTGTCAAATACTCTCTTTTCAAGGAACCCGAGAAGTATGGATTTGAGCTCCCACTTGTTGCTTGTTGTGGCTACGGTGGTAAGTACAACTATAACGCCACTGCTGAATGTGGAGCAATAATTACAGTCAATGGAAGCCAAATCTTTGTTGGTTCATGCAAAAACCCCTCAACTAGAGTGATCTGGGATGGTATTCATTACACCGAGGCAGGTGCGAAATTTATTTTTGATAAAATTTCAACTGGAGCATATTCAGATCCACCTCTTCCCTTGAAACAAGCATGTCATGGGAGTTTGAACTCTGAATTAAAGAATAACTTGGTGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005488 GO:0006950 GO:0006952 GO:0008150 GO:0016787 GO:0016788 GO:0019863 GO:0019865 GO:0044877 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

394

Amino Acids

43.21

Weight (kDa)

7.14

Isoelectric Point (pI)

33.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 36 - 363 3.2e-48 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1052
Acc36I ACCTGC 1 cut(s) 1052
Acc65I GGTACC 1 cut(s) 6
AccB1I GGYRCC 1 cut(s) 6
AccB7I CCANNNNNTGG 1 cut(s) 374
AciI CCGC 1 cut(s) 150
AclWI GGATC 4 cut(s) 655, 668, 822, 1103
AcsI RAATTY 5 cut(s) 127, 301, 319, 1070, 1086
AcuI CTGAAG 1 cut(s) 806
AfaI GTAC 3 cut(s) 8, 73, 938
AfiI CCNNNNNNNGG 3 cut(s) 202, 374, 670
AgeI ACCGGT 1 cut(s) 605
AgsI TTSAA 8 cut(s) 85, 112, 404, 803, 874, 1092, 1126, 1150
AjnI CCWGG 1 cut(s) 243
AluBI AGCT 7 cut(s) 80, 335, 506, 719, 764, 784, 901
AluI AGCT 7 cut(s) 80, 335, 506, 719, 764, 784, 901
Alw21I GWGCWC 1 cut(s) 903
Alw26I GTCTC 3 cut(s) 114, 179, 692
AlwI GGATC 4 cut(s) 655, 668, 822, 1103
AlwNI CAGNNNCTG 2 cut(s) 137, 418
Ama87I CYCGRG 2 cut(s) 293, 881
AoxI GGCC 1 cut(s) 674
ApeKI GCWGC 2 cut(s) 332, 827
ApoI RAATTY 5 cut(s) 127, 301, 319, 1070, 1086
ArsI GACNNNNNNTTYG 2 cut(s) 698, 730
AsiGI ACCGGT 1 cut(s) 605
Asp718I GGTACC 1 cut(s) 6
AspLEI GCGC 1 cut(s) 278
AspS9I GGNCC 1 cut(s) 674
AsuHPI GGTGA 2 cut(s) 254, 595
AvaI CYCGRG 2 cut(s) 293, 881
AxyI CCTNAGG 1 cut(s) 822
BamHI GGATCC 1 cut(s) 660
BanI GGYRCC 1 cut(s) 6
BanII GRGCYC 1 cut(s) 903
BauI CACGAG 1 cut(s) 779
BbsI GAAGAC 1 cut(s) 232
Bbv12I GWGCWC 1 cut(s) 903
BbvI GCAGC 2 cut(s) 344, 814
BccI CCATC 3 cut(s) 113, 215, 1034
BcgI CGANNNNNNTGC 2 cut(s) 1046, 1080
BciT130I CCWGG 1 cut(s) 245
BcoDI GTCTC 3 cut(s) 114, 179, 692
BfaI CTAG 3 cut(s) 15, 146, 1026
BfmI CTRYAG 1 cut(s) 330
BfuAI ACCTGC 1 cut(s) 1052
BglII AGATCT 1 cut(s) 355
BisI GCNGC 3 cut(s) 150, 333, 828
BlsI GCNGC 3 cut(s) 151, 334, 829
Bme1390I CCNGG 1 cut(s) 245
BmeT110I CYCGRG 2 cut(s) 293, 881
BmgT120I GGNCC 1 cut(s) 674
BmiI GGNNCC 4 cut(s) 8, 662, 675, 879
BmrFI CCNGG 1 cut(s) 245
BmsI GCATC 3 cut(s) 161, 460, 593
BoxI GACNNNNGTC 1 cut(s) 525
BpiI GAAGAC 1 cut(s) 232
BpmI CTGGAG 1 cut(s) 1116
Bpu10I CCTNAGC 1 cut(s) 76
Bsa29I ATCGAT 1 cut(s) 237
BsaI GGTCTC 1 cut(s) 179
BsaJI CCNNGG 1 cut(s) 1056
BsaWI WCCGGW 1 cut(s) 605
Bsc4I CCNNNNNNNGG 3 cut(s) 202, 374, 670
Bse118I RCCGGY 3 cut(s) 202, 371, 605
Bse1I ACTGG 3 cut(s) 66, 142, 1099
Bse21I CCTNAGG 1 cut(s) 822
BseBI CCWGG 1 cut(s) 245
BseCI ATCGAT 1 cut(s) 237
BseDI CCNNGG 1 cut(s) 1056
BseGI GGATG 2 cut(s) 152, 1045
BseLI CCNNNNNNNGG 3 cut(s) 202, 374, 670
BseMII CTCAG 2 cut(s) 711, 820
BseNI ACTGG 3 cut(s) 66, 142, 1099
BseXI GCAGC 2 cut(s) 344, 814
BsgI GTGCAG 1 cut(s) 431
Bsh1285I CGRYCG 1 cut(s) 207
BshFI GGCC 1 cut(s) 676
BshNI GGYRCC 1 cut(s) 6
BshTI ACCGGT 1 cut(s) 605
BshVI ATCGAT 1 cut(s) 237
BsiEI CGRYCG 1 cut(s) 207
BsiHKAI GWGCWC 1 cut(s) 903
BsiHKCI CYCGRG 2 cut(s) 293, 881
BsiSI CCGG 3 cut(s) 203, 372, 606
BslFI GGGAC 1 cut(s) 310
BslI CCNNNNNNNGG 3 cut(s) 202, 374, 670
BsmAI GTCTC 3 cut(s) 114, 179, 692
BsmFI GGGAC 1 cut(s) 310
BsmI GAATGC 1 cut(s) 830
BsnI GGCC 1 cut(s) 676
Bso31I GGTCTC 1 cut(s) 179
BsoBI CYCGRG 2 cut(s) 293, 881
Bsp1286I GDGCHC 1 cut(s) 903
Bsp143I GATC 8 cut(s) 355, 434, 535, 650, 660, 814, 1032, 1108
BspACI CCGC 1 cut(s) 150
BspANI GGCC 1 cut(s) 676
BspCNI CTCAG 2 cut(s) 712, 819
BspDI ATCGAT 1 cut(s) 237
BspLI GGNNCC 4 cut(s) 8, 662, 675, 879
BspMAI CTGCAG 1 cut(s) 334
BspMI ACCTGC 1 cut(s) 1052
BspPI GGATC 4 cut(s) 655, 668, 822, 1103
BspT107I GGYRCC 1 cut(s) 6
BspTNI GGTCTC 1 cut(s) 179
BsrFI RCCGGY 3 cut(s) 202, 371, 605
BsrI ACTGG 3 cut(s) 66, 142, 1099
BssAI RCCGGY 3 cut(s) 202, 371, 605
BssECI CCNNGG 1 cut(s) 1056
BssMI GATC 8 cut(s) 355, 434, 535, 650, 660, 814, 1032, 1108
BssSI CACGAG 1 cut(s) 779
Bst2BI CACGAG 1 cut(s) 779
Bst2UI CCWGG 1 cut(s) 245
Bst4CI ACNGT 2 cut(s) 929, 979
Bst6I CTCTTC 1 cut(s) 1122
BstAPI GCANNNNNTGC 1 cut(s) 418
BstC8I GCNNGC 1 cut(s) 792
BstDEI CTNAG 5 cut(s) 76, 272, 720, 806, 822
BstEII GGTNACC 1 cut(s) 260
BstF5I GGATG 2 cut(s) 152, 1045
BstHHI GCGC 1 cut(s) 278
BstKTI GATC 8 cut(s) 358, 437, 538, 653, 663, 817, 1035, 1111
BstMAI GTCTC 3 cut(s) 114, 179, 692
BstMBI GATC 8 cut(s) 355, 434, 535, 650, 660, 814, 1032, 1108
BstMCI CGRYCG 1 cut(s) 207
BstMWI GCNNNNNNNGC 4 cut(s) 55, 323, 332, 418
BstNI CCWGG 1 cut(s) 245
BstNSI RCATGY 1 cut(s) 1137
BstPAI GACNNNNGTC 1 cut(s) 525
BstPI GGTNACC 1 cut(s) 260
BstSCI CCNGG 1 cut(s) 243
BstSFI CTRYAG 1 cut(s) 330
BstV1I GCAGC 2 cut(s) 344, 814
BstV2I GAAGAC 1 cut(s) 232
BstX2I RGATCY 4 cut(s) 355, 660, 814, 1108
BstYI RGATCY 4 cut(s) 355, 660, 814, 1108
Bsu15I ATCGAT 1 cut(s) 237
Bsu36I CCTNAGG 1 cut(s) 822
BsuRI GGCC 1 cut(s) 676
BsuTUI ATCGAT 1 cut(s) 237
BtsCI GGATG 2 cut(s) 152, 1045
BtsI GCAGTG 2 cut(s) 327, 951
BtsIMutI CAGTG 2 cut(s) 327, 951
BveI ACCTGC 1 cut(s) 1052
Cac8I GCNNGC 1 cut(s) 792
CaiI CAGNNNCTG 2 cut(s) 137, 418
CfoI GCGC 1 cut(s) 278
Cfr10I RCCGGY 3 cut(s) 202, 371, 605
Cfr13I GGNCC 1 cut(s) 674
ClaI ATCGAT 1 cut(s) 237
Csp6I GTAC 3 cut(s) 7, 72, 937
CspAI ACCGGT 1 cut(s) 605
CviAII CATG 5 cut(s) 311, 562, 1008, 1134, 1139
CviQI GTAC 3 cut(s) 7, 72, 937
DdeI CTNAG 5 cut(s) 76, 272, 720, 806, 822
DpnI GATC 8 cut(s) 357, 436, 537, 652, 662, 816, 1034, 1110
DpnII GATC 8 cut(s) 355, 434, 535, 650, 660, 814, 1032, 1108
Eam1104I CTCTTC 1 cut(s) 1122
EarI CTCTTC 1 cut(s) 1122
Ecl136II GAGCTC 1 cut(s) 901
Eco24I GRGCYC 1 cut(s) 903
Eco31I GGTCTC 1 cut(s) 179
Eco53kI GAGCTC 1 cut(s) 901
Eco57I CTGAAG 1 cut(s) 806
Eco81I CCTNAGG 1 cut(s) 822
Eco88I CYCGRG 2 cut(s) 293, 881
Eco91I GGTNACC 1 cut(s) 260
EcoICRI GAGCTC 1 cut(s) 901
EcoO65I GGTNACC 1 cut(s) 260
EcoRII CCWGG 1 cut(s) 243
EcoT22I ATGCAT 1 cut(s) 586
EcoT38I GRGCYC 1 cut(s) 903
FaeI CATG 5 cut(s) 314, 565, 1011, 1137, 1142
FalI AAGNNNNNCTT 3 cut(s) 479, 511, 1154
FaqI GGGAC 1 cut(s) 310
FatI CATG 5 cut(s) 310, 561, 1007, 1133, 1138
FauNDI CATATG 1 cut(s) 198
Fnu4HI GCNGC 3 cut(s) 150, 333, 828
FokI GGATG 2 cut(s) 139, 1052
FriOI GRGCYC 1 cut(s) 903
Fsp4HI GCNGC 3 cut(s) 150, 333, 828
FspBI CTAG 3 cut(s) 15, 146, 1026
GlaI GCGC 1 cut(s) 277
GluI GCNGC 3 cut(s) 150, 333, 828
GsuI CTGGAG 1 cut(s) 1116
HaeIII GGCC 1 cut(s) 676
HapII CCGG 3 cut(s) 203, 372, 606
HhaI GCGC 1 cut(s) 278
Hin1II CATG 5 cut(s) 314, 565, 1011, 1137, 1142
Hin6I GCGC 1 cut(s) 276
HinP1I GCGC 1 cut(s) 276
HindIII AAGCTT 1 cut(s) 504
HinfI GANTC 6 cut(s) 122, 213, 228, 253, 287, 348
HpaII CCGG 3 cut(s) 203, 372, 606
HphI GGTGA 2 cut(s) 254, 595
Hpy166II GTNNAC 2 cut(s) 143, 476
Hpy188I TCNGA 8 cut(s) 119, 133, 209, 220, 347, 531, 1108, 1156
Hpy188III TCNNGA 6 cut(s) 284, 295, 432, 593, 625, 808
Hpy8I GTNNAC 2 cut(s) 143, 476
HpyAV CCTTC 3 cut(s) 167, 481, 805
HpyCH4III ACNGT 2 cut(s) 929, 979
HpyF10VI GCNNNNNNNGC 4 cut(s) 55, 323, 332, 418
HpyF3I CTNAG 5 cut(s) 76, 272, 720, 806, 822
Hsp92II CATG 5 cut(s) 314, 565, 1011, 1137, 1142
HspAI GCGC 1 cut(s) 276
KpnI GGTACC 1 cut(s) 10
Kzo9I GATC 8 cut(s) 355, 434, 535, 650, 660, 814, 1032, 1108
LmnI GCTCC 5 cut(s) 44, 644, 906, 965, 1097
Lsp1109I GCAGC 2 cut(s) 344, 814
LweI GCATC 3 cut(s) 161, 460, 593
MaeI CTAG 3 cut(s) 15, 146, 1026
MaeIII GTNAC 2 cut(s) 260, 557
MalI GATC 8 cut(s) 357, 436, 537, 652, 662, 816, 1034, 1110
MboI GATC 8 cut(s) 355, 434, 535, 650, 660, 814, 1032, 1108
MboII GAAGA 5 cut(s) 100, 222, 237, 809, 1109
MflI RGATCY 4 cut(s) 355, 660, 814, 1108
MhlI GDGCHC 1 cut(s) 903
MluCI AATT 7 cut(s) 127, 301, 319, 972, 1070, 1086, 1157
MlyI GAGTC 4 cut(s) 116, 222, 262, 342
MmeI TCCRAC 1 cut(s) 187
MnlI CCTC 6 cut(s) 21, 449, 538, 1030, 1051, 1125
Mph1103I ATGCAT 1 cut(s) 586
MseI TTAA 3 cut(s) 579, 774, 1160
MspA1I CMGCKG 1 cut(s) 719
MspI CCGG 3 cut(s) 203, 372, 606
MspR9I CCNGG 1 cut(s) 245
Mva1269I GAATGC 1 cut(s) 830
MvaI CCWGG 1 cut(s) 245
MwoI GCNNNNNNNGC 4 cut(s) 55, 323, 332, 418
NdeI CATATG 1 cut(s) 198
NdeII GATC 8 cut(s) 355, 434, 535, 650, 660, 814, 1032, 1108
NlaIII CATG 5 cut(s) 314, 565, 1011, 1137, 1142
NlaIV GGNNCC 4 cut(s) 8, 662, 675, 879
NmuCI GTSAC 1 cut(s) 260
NsiI ATGCAT 1 cut(s) 586
NspI RCATGY 1 cut(s) 1137
PaqCI CACCTGC 1 cut(s) 1052
PctI GAATGC 1 cut(s) 830
PfeI GAWTC 2 cut(s) 213, 287
PflMI CCANNNNNTGG 1 cut(s) 374
PinAI ACCGGT 1 cut(s) 605
PkrI GCNGC 3 cut(s) 151, 334, 829
PleI GAGTC 4 cut(s) 116, 222, 261, 342
PpsI GAGTC 4 cut(s) 116, 222, 261, 342
PshAI GACNNNNGTC 1 cut(s) 525
Psp124BI GAGCTC 1 cut(s) 903
Psp6I CCWGG 1 cut(s) 243
PspEI GGTNACC 1 cut(s) 260
PspGI CCWGG 1 cut(s) 243
PspN4I GGNNCC 4 cut(s) 8, 662, 675, 879
PspPI GGNCC 1 cut(s) 674
PstI CTGCAG 1 cut(s) 334
PstNI CAGNNNCTG 2 cut(s) 137, 418
PsuI RGATCY 4 cut(s) 355, 660, 814, 1108
PvuII CAGCTG 1 cut(s) 719
RsaI GTAC 3 cut(s) 8, 73, 938
RsaNI GTAC 3 cut(s) 7, 72, 937
SacI GAGCTC 1 cut(s) 903
SaqAI TTAA 3 cut(s) 579, 774, 1160
SatI GCNGC 3 cut(s) 150, 333, 828
Sau3AI GATC 8 cut(s) 355, 434, 535, 650, 660, 814, 1032, 1108
Sau96I GGNCC 1 cut(s) 674
SchI GAGTC 4 cut(s) 116, 222, 262, 342
ScrFI CCNGG 1 cut(s) 245
SduI GDGCHC 1 cut(s) 903
SfaNI GCATC 3 cut(s) 161, 460, 593
SfcI CTRYAG 1 cut(s) 330
Sse9I AATT 7 cut(s) 127, 301, 319, 972, 1070, 1086, 1157
SsiI CCGC 1 cut(s) 150
SspI AATATT 1 cut(s) 465
SspMI CTAG 3 cut(s) 15, 146, 1026
SstI GAGCTC 1 cut(s) 903
StyD4I CCNGG 1 cut(s) 243
TaaI ACNGT 2 cut(s) 929, 979
TaqI TCGA 3 cut(s) 237, 454, 519
TasI AATT 7 cut(s) 127, 301, 319, 972, 1070, 1086, 1157
TatI WGTACW 1 cut(s) 936
TauI GCSGC 1 cut(s) 152
TfiI GAWTC 2 cut(s) 213, 287
Tru1I TTAA 3 cut(s) 579, 774, 1160
Tru9I TTAA 3 cut(s) 579, 774, 1160
TscAI CASTG 2 cut(s) 334, 958
TseFI GTSAC 1 cut(s) 260
TseI GCWGC 2 cut(s) 332, 827
Tsp45I GTSAC 1 cut(s) 260
TspDTI ATGAA 3 cut(s) 771, 996, 1037
TspRI CASTG 2 cut(s) 334, 958
Van91I CCANNNNNTGG 1 cut(s) 374
XapI RAATTY 5 cut(s) 127, 301, 319, 1070, 1086
XceI RCATGY 1 cut(s) 1137
XcmI CCANNNNNNNNNTGG 1 cut(s) 998
XspI CTAG 3 cut(s) 15, 146, 1026
Zsp2I ATGCAT 1 cut(s) 586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.