RLG00000011770

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000978
Physical Location & Seq
Forward (+)
12489958 .. 12492466
2509 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011770

Sequence Viewer

Length: 1161 bp
ATGTGGTTGGGGTACTCTCCTAAAGTTATCATTTCTCTGTTCTGCTCCTATATGCTTATATTGCATATTACCAGTAGTACCTTGGCTTTGAAAGACTGTGAGTTTCCTGCCATCTTCAACTTCGGAGACTCAAATTCAGATACTGGTGGACTAGCCGCATCCCTTCTACCACCAACCACACCTTATGGAGAGACCTATTTCCATATGCCGGCCGGAAGATTCTCTGATGGAAGACTCATCATCGATTTCCTTGCAAAGAGTCTTGGTCACCCTTATCTAAGCGCATATCTGGATTCTCTCGGGACAAATTTCTCGTATGGTGCAAATTTTGCAACTGCAGCTTCCACTATCAGACTCCCAGATCTCATTATACCAGCCGGTGGATTTAGTCCCTTCTACCTTGATATTCAATATATGCAGTTCCTGCAACTAAAATCCAGATCACAACTCATAAGGCATCGAGGGGGAATATTTGCAAGTTTAATGCCCAAGGAGGAGTATTTCTCCAAAGCTTTATACACATTCGACATTGGCCAGAATGATCTTGGCGAGGGATTTTTCGGTAACCTGACTATACAGGAAGTAAATGCATCTGTTCCTGATATAATCACTGGTTTCTCAACGAATATCAAGAAAATATATGATTTGGGAGCAAGATCATTATGGATCCACAATACAGGGCCGATTGGCTGTCTCCCTTACGTTCTGGCAAACTTCCCATCAGCTCAGAAAGATGAGATTGGCTGTGCAAAGGCTTACAATGAAGTAGCTGAACATTTTAACCATGAGCTGAAGCAGGCCATAGTTCAACTCAGGAAGGATCTTCCTAAGGCAGCATTTACTTATGTAGATGTGTATTCTGTCAAATACTCTCTTTTCAAGGAACCCGAGAAATATGGATTTGAGCTCCCACTTGTTGCTTGTTGTGGCTACGGTGGTAAGTACAACTATAACGCCACTGCTGAATGTGGAGCAATAATTACAGTCAATGGAAGCCAAATCTTTGTTGGTTCATGCAAAAACCCCTCAACTAGAGTTGTCTGGGATGGTATTCATTACACCGAGGCAGCTGCGAAATTTATTTTTGATAAAATTTCAACTGGAGCATATTCAGATCCACCTCTTCCCTTGAAACAAGCATGTCAAAGGAGTTTGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005488 GO:0006950 GO:0006952 GO:0008150 GO:0016787 GO:0016788 GO:0019863 GO:0019865 GO:0044877 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

387

Amino Acids

42.58

Weight (kDa)

6.45

Isoelectric Point (pI)

38.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 38 - 365 2.1e-47 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 380
AciI CCGC 1 cut(s) 156
AclWI GGATC 4 cut(s) 661, 674, 828, 1109
AcoI YGGCCR 2 cut(s) 210, 532
AcsI RAATTY 5 cut(s) 133, 307, 325, 1076, 1092
AcuI CTGAAG 1 cut(s) 812
AfaI GTAC 3 cut(s) 14, 79, 944
AfiI CCNNNNNNNGG 2 cut(s) 208, 380
AgsI TTSAA 8 cut(s) 91, 118, 410, 809, 880, 1098, 1132, 1156
AluBI AGCT 7 cut(s) 341, 512, 725, 770, 790, 907, 1070
AluI AGCT 7 cut(s) 341, 512, 725, 770, 790, 907, 1070
Alw21I GWGCWC 1 cut(s) 909
Alw26I GTCTC 3 cut(s) 120, 185, 698
AlwI GGATC 4 cut(s) 661, 674, 828, 1109
AlwNI CAGNNNCTG 2 cut(s) 143, 424
Ama87I CYCGRG 2 cut(s) 299, 887
AoxI GGCC 4 cut(s) 210, 532, 680, 798
ApeKI GCWGC 4 cut(s) 338, 833, 1067, 1070
ApoI RAATTY 5 cut(s) 133, 307, 325, 1076, 1092
AspLEI GCGC 1 cut(s) 284
AspS9I GGNCC 1 cut(s) 680
AsuHPI GGTGA 1 cut(s) 260
AvaI CYCGRG 2 cut(s) 299, 887
AxyI CCTNAGG 1 cut(s) 828
BalI TGGCCA 1 cut(s) 534
BamHI GGATCC 1 cut(s) 666
BanII GRGCYC 1 cut(s) 909
BbsI GAAGAC 1 cut(s) 238
Bbv12I GWGCWC 1 cut(s) 909
BbvI GCAGC 4 cut(s) 350, 845, 1057, 1079
BccI CCATC 4 cut(s) 119, 221, 727, 1040
BcgI CGANNNNNNTGC 4 cut(s) 233, 267, 1052, 1086
BcoDI GTCTC 3 cut(s) 120, 185, 698
BfaI CTAG 2 cut(s) 152, 1032
BfmI CTRYAG 1 cut(s) 336
BglII AGATCT 1 cut(s) 361
BisI GCNGC 5 cut(s) 156, 339, 834, 1068, 1071
BlsI GCNGC 5 cut(s) 157, 340, 835, 1069, 1072
BmeT110I CYCGRG 2 cut(s) 299, 887
BmgT120I GGNCC 1 cut(s) 680
BmiI GGNNCC 2 cut(s) 668, 885
BmsI GCATC 3 cut(s) 167, 466, 599
BpiI GAAGAC 1 cut(s) 238
BplI GAGNNNNNCTC 2 cut(s) 488, 520
BpmI CTGGAG 1 cut(s) 1122
Bsa29I ATCGAT 1 cut(s) 243
BsaI GGTCTC 1 cut(s) 185
BsaJI CCNNGG 3 cut(s) 81, 489, 1062
Bsc4I CCNNNNNNNGG 2 cut(s) 208, 380
Bse118I RCCGGY 2 cut(s) 208, 377
Bse1I ACTGG 4 cut(s) 72, 148, 616, 1105
Bse21I CCTNAGG 1 cut(s) 828
BseCI ATCGAT 1 cut(s) 243
BseDI CCNNGG 3 cut(s) 81, 489, 1062
BseGI GGATG 2 cut(s) 158, 1051
BseLI CCNNNNNNNGG 2 cut(s) 208, 380
BseMII CTCAG 2 cut(s) 740, 826
BseNI ACTGG 4 cut(s) 72, 148, 616, 1105
BseRI GAGGAG 1 cut(s) 509
BseX3I CGGCCG 1 cut(s) 210
BseXI GCAGC 4 cut(s) 350, 845, 1057, 1079
Bsh1285I CGRYCG 1 cut(s) 213
BshFI GGCC 4 cut(s) 212, 534, 682, 800
BshVI ATCGAT 1 cut(s) 243
BsiEI CGRYCG 1 cut(s) 213
BsiHKAI GWGCWC 1 cut(s) 909
BsiHKCI CYCGRG 2 cut(s) 299, 887
BsiSI CCGG 3 cut(s) 209, 213, 378
BslFI GGGAC 2 cut(s) 316, 375
BslI CCNNNNNNNGG 2 cut(s) 208, 380
BsmAI GTCTC 3 cut(s) 120, 185, 698
BsmFI GGGAC 2 cut(s) 316, 375
BsnI GGCC 4 cut(s) 212, 534, 682, 800
Bso31I GGTCTC 1 cut(s) 185
BsoBI CYCGRG 2 cut(s) 299, 887
Bsp1286I GDGCHC 1 cut(s) 909
Bsp143I GATC 7 cut(s) 361, 440, 541, 656, 666, 820, 1114
BspACI CCGC 1 cut(s) 156
BspANI GGCC 4 cut(s) 212, 534, 682, 800
BspCNI CTCAG 2 cut(s) 739, 825
BspDI ATCGAT 1 cut(s) 243
BspLI GGNNCC 2 cut(s) 668, 885
BspMAI CTGCAG 1 cut(s) 340
BspPI GGATC 4 cut(s) 661, 674, 828, 1109
BspTNI GGTCTC 1 cut(s) 185
BsrFI RCCGGY 2 cut(s) 208, 377
BsrI ACTGG 4 cut(s) 72, 148, 616, 1105
BssAI RCCGGY 2 cut(s) 208, 377
BssECI CCNNGG 3 cut(s) 81, 489, 1062
BssMI GATC 7 cut(s) 361, 440, 541, 656, 666, 820, 1114
BssT1I CCWWGG 2 cut(s) 81, 489
Bst4CI ACNGT 3 cut(s) 98, 935, 985
Bst6I CTCTTC 1 cut(s) 1128
BstAPI GCANNNNNTGC 2 cut(s) 329, 424
BstC8I GCNNGC 2 cut(s) 210, 798
BstDEI CTNAG 4 cut(s) 278, 726, 812, 828
BstEII GGTNACC 2 cut(s) 266, 563
BstF5I GGATG 2 cut(s) 158, 1051
BstHHI GCGC 1 cut(s) 284
BstKTI GATC 7 cut(s) 364, 443, 544, 659, 669, 823, 1117
BstMAI GTCTC 3 cut(s) 120, 185, 698
BstMBI GATC 7 cut(s) 361, 440, 541, 656, 666, 820, 1114
BstMCI CGRYCG 1 cut(s) 213
BstMWI GCNNNNNNNGC 4 cut(s) 61, 329, 338, 424
BstNSI RCATGY 1 cut(s) 1143
BstPI GGTNACC 2 cut(s) 266, 563
BstSFI CTRYAG 1 cut(s) 336
BstV1I GCAGC 4 cut(s) 350, 845, 1057, 1079
BstV2I GAAGAC 1 cut(s) 238
BstX2I RGATCY 4 cut(s) 361, 666, 820, 1114
BstYI RGATCY 4 cut(s) 361, 666, 820, 1114
BstZI CGGCCG 1 cut(s) 210
Bsu15I ATCGAT 1 cut(s) 243
Bsu36I CCTNAGG 1 cut(s) 828
BsuRI GGCC 4 cut(s) 212, 534, 682, 800
BsuTUI ATCGAT 1 cut(s) 243
BtsCI GGATG 2 cut(s) 158, 1051
BtsI GCAGTG 1 cut(s) 957
BtsIMutI CAGTG 2 cut(s) 609, 957
Cac8I GCNNGC 2 cut(s) 210, 798
CaiI CAGNNNCTG 2 cut(s) 143, 424
CfoI GCGC 1 cut(s) 284
Cfr10I RCCGGY 2 cut(s) 208, 377
Cfr13I GGNCC 1 cut(s) 680
ClaI ATCGAT 1 cut(s) 243
Csp6I GTAC 3 cut(s) 13, 78, 943
CviAII CATG 3 cut(s) 785, 1014, 1140
CviQI GTAC 3 cut(s) 13, 78, 943
DdeI CTNAG 4 cut(s) 278, 726, 812, 828
DpnI GATC 7 cut(s) 363, 442, 543, 658, 668, 822, 1116
DpnII GATC 7 cut(s) 361, 440, 541, 656, 666, 820, 1114
EaeI YGGCCR 2 cut(s) 210, 532
EagI CGGCCG 1 cut(s) 210
Eam1104I CTCTTC 1 cut(s) 1128
EarI CTCTTC 1 cut(s) 1128
Ecl136II GAGCTC 1 cut(s) 907
EclXI CGGCCG 1 cut(s) 210
Eco130I CCWWGG 2 cut(s) 81, 489
Eco24I GRGCYC 1 cut(s) 909
Eco31I GGTCTC 1 cut(s) 185
Eco52I CGGCCG 1 cut(s) 210
Eco53kI GAGCTC 1 cut(s) 907
Eco57I CTGAAG 1 cut(s) 812
Eco81I CCTNAGG 1 cut(s) 828
Eco88I CYCGRG 2 cut(s) 299, 887
Eco91I GGTNACC 2 cut(s) 266, 563
EcoICRI GAGCTC 1 cut(s) 907
EcoO65I GGTNACC 2 cut(s) 266, 563
EcoT14I CCWWGG 2 cut(s) 81, 489
EcoT22I ATGCAT 1 cut(s) 592
EcoT38I GRGCYC 1 cut(s) 909
ErhI CCWWGG 2 cut(s) 81, 489
FaeI CATG 3 cut(s) 788, 1017, 1143
FaqI GGGAC 2 cut(s) 316, 375
FatI CATG 3 cut(s) 784, 1013, 1139
FauNDI CATATG 1 cut(s) 204
Fnu4HI GCNGC 5 cut(s) 156, 339, 834, 1068, 1071
FokI GGATG 2 cut(s) 145, 1058
FriOI GRGCYC 1 cut(s) 909
Fsp4HI GCNGC 5 cut(s) 156, 339, 834, 1068, 1071
FspBI CTAG 2 cut(s) 152, 1032
GlaI GCGC 1 cut(s) 283
GluI GCNGC 5 cut(s) 156, 339, 834, 1068, 1071
GsuI CTGGAG 1 cut(s) 1122
HaeIII GGCC 4 cut(s) 212, 534, 682, 800
HapII CCGG 3 cut(s) 209, 213, 378
HhaI GCGC 1 cut(s) 284
Hin1II CATG 3 cut(s) 788, 1017, 1143
Hin6I GCGC 1 cut(s) 282
HinP1I GCGC 1 cut(s) 282
HindIII AAGCTT 1 cut(s) 510
HinfI GANTC 6 cut(s) 128, 219, 234, 259, 293, 354
HpaII CCGG 3 cut(s) 209, 213, 378
HphI GGTGA 1 cut(s) 260
Hpy166II GTNNAC 1 cut(s) 149
Hpy188I TCNGA 6 cut(s) 125, 139, 226, 353, 729, 1114
Hpy188III TCNNGA 6 cut(s) 290, 301, 438, 599, 631, 814
Hpy8I GTNNAC 1 cut(s) 149
HpyAV CCTTC 3 cut(s) 173, 403, 811
HpyCH4III ACNGT 3 cut(s) 98, 935, 985
HpyCH4IV ACGT 1 cut(s) 702
HpyF10VI GCNNNNNNNGC 4 cut(s) 61, 329, 338, 424
HpyF3I CTNAG 4 cut(s) 278, 726, 812, 828
HpySE526I ACGT 1 cut(s) 702
Hsp92II CATG 3 cut(s) 788, 1017, 1143
HspAI GCGC 1 cut(s) 282
KroI GCCGGC 1 cut(s) 208
KroNI GCCGGC 1 cut(s) 210
Kzo9I GATC 7 cut(s) 361, 440, 541, 656, 666, 820, 1114
LmnI GCTCC 5 cut(s) 50, 650, 912, 971, 1103
Lsp1109I GCAGC 4 cut(s) 350, 845, 1057, 1079
LweI GCATC 3 cut(s) 167, 466, 599
MaeI CTAG 2 cut(s) 152, 1032
MaeII ACGT 1 cut(s) 702
MaeIII GTNAC 2 cut(s) 266, 563
MalI GATC 7 cut(s) 363, 442, 543, 658, 668, 822, 1116
MboI GATC 7 cut(s) 361, 440, 541, 656, 666, 820, 1114
MboII GAAGA 5 cut(s) 106, 228, 243, 815, 1115
MflI RGATCY 4 cut(s) 361, 666, 820, 1114
MhlI GDGCHC 1 cut(s) 909
MlsI TGGCCA 1 cut(s) 534
MluCI AATT 6 cut(s) 133, 307, 325, 978, 1076, 1092
MluNI TGGCCA 1 cut(s) 534
MlyI GAGTC 4 cut(s) 122, 228, 268, 348
MnlI CCTC 6 cut(s) 455, 487, 544, 1036, 1057, 1131
Mox20I TGGCCA 1 cut(s) 534
Mph1103I ATGCAT 1 cut(s) 592
MroNI GCCGGC 1 cut(s) 208
MscI TGGCCA 1 cut(s) 534
MseI TTAA 2 cut(s) 482, 780
Msp20I TGGCCA 1 cut(s) 534
MspA1I CMGCKG 1 cut(s) 1070
MspI CCGG 3 cut(s) 209, 213, 378
MwoI GCNNNNNNNGC 4 cut(s) 61, 329, 338, 424
NaeI GCCGGC 1 cut(s) 210
NdeI CATATG 1 cut(s) 204
NdeII GATC 7 cut(s) 361, 440, 541, 656, 666, 820, 1114
NgoMIV GCCGGC 1 cut(s) 208
NlaIII CATG 3 cut(s) 788, 1017, 1143
NlaIV GGNNCC 2 cut(s) 668, 885
NmuCI GTSAC 1 cut(s) 266
NsiI ATGCAT 1 cut(s) 592
NspI RCATGY 1 cut(s) 1143
PdiI GCCGGC 1 cut(s) 210
PfeI GAWTC 2 cut(s) 219, 293
PflMI CCANNNNNTGG 1 cut(s) 380
PkrI GCNGC 5 cut(s) 157, 340, 835, 1069, 1072
PleI GAGTC 4 cut(s) 122, 228, 267, 348
PpsI GAGTC 4 cut(s) 122, 228, 267, 348
Psp124BI GAGCTC 1 cut(s) 909
PspEI GGTNACC 2 cut(s) 266, 563
PspN4I GGNNCC 2 cut(s) 668, 885
PspPI GGNCC 1 cut(s) 680
PstI CTGCAG 1 cut(s) 340
PstNI CAGNNNCTG 2 cut(s) 143, 424
PsuI RGATCY 4 cut(s) 361, 666, 820, 1114
PvuII CAGCTG 1 cut(s) 1070
RsaI GTAC 3 cut(s) 14, 79, 944
RsaNI GTAC 3 cut(s) 13, 78, 943
SacI GAGCTC 1 cut(s) 909
SaqAI TTAA 2 cut(s) 482, 780
SatI GCNGC 5 cut(s) 156, 339, 834, 1068, 1071
Sau3AI GATC 7 cut(s) 361, 440, 541, 656, 666, 820, 1114
Sau96I GGNCC 1 cut(s) 680
SchI GAGTC 4 cut(s) 122, 228, 268, 348
SduI GDGCHC 1 cut(s) 909
SfaNI GCATC 3 cut(s) 167, 466, 599
SfcI CTRYAG 1 cut(s) 336
Sse9I AATT 6 cut(s) 133, 307, 325, 978, 1076, 1092
SsiI CCGC 1 cut(s) 156
SspI AATATT 1 cut(s) 471
SspMI CTAG 2 cut(s) 152, 1032
SstI GAGCTC 1 cut(s) 909
StyI CCWWGG 2 cut(s) 81, 489
TaaI ACNGT 3 cut(s) 98, 935, 985
TaiI ACGT 1 cut(s) 705
TaqI TCGA 3 cut(s) 243, 460, 525
TasI AATT 6 cut(s) 133, 307, 325, 978, 1076, 1092
TatI WGTACW 1 cut(s) 942
TauI GCSGC 1 cut(s) 158
TfiI GAWTC 2 cut(s) 219, 293
Tru1I TTAA 2 cut(s) 482, 780
Tru9I TTAA 2 cut(s) 482, 780
TscAI CASTG 2 cut(s) 616, 964
TseFI GTSAC 1 cut(s) 266
TseI GCWGC 4 cut(s) 338, 833, 1067, 1070
Tsp45I GTSAC 1 cut(s) 266
TspDTI ATGAA 3 cut(s) 777, 1002, 1043
TspRI CASTG 2 cut(s) 616, 964
Van91I CCANNNNNTGG 1 cut(s) 380
XapI RAATTY 5 cut(s) 133, 307, 325, 1076, 1092
XceI RCATGY 1 cut(s) 1143
XcmI CCANNNNNNNNNTGG 3 cut(s) 79, 542, 1004
XspI CTAG 2 cut(s) 152, 1032
Zsp2I ATGCAT 1 cut(s) 592
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.