MD15G1349000.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000978
Physical Location & Seq
Forward (+)
41348202 .. 41349446
1245 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1349000.v1.1.491

Sequence Viewer

Length: 546 bp
ATGTTGGTTTGCCAGTATTTTGAAAAAATATACAGATTGGGAGCCAGATCGTTTTGGATCCACAACACTGGGCCAATTGGCTGTCTCCCTTATATTTTAGCTAATTTTCCATCAGCTCAAAAGGACGAGGTTGGCTGCGCAAAGTCTTACAATGAAGTGGCTCAGTATTTTAACCACCAACTGAAGGAAGCCACAGTTCAACTTAGGAAGGATCTTCCTTTGGCTGCATTTACTTACGTGGACATATATTCTGTCAAATACTCTCTTTACAAGGAGCCAAAAAAATATGGGTTTGAGTTCCCACTTGTTGCTTGTTGTGGGTTTGGTGGGAAGTACAACTATAGCAGTAGTGCTGGATGTGGAACAACAGTTACAGTCAATGGAAGCCAAATATTTGTTGGTTCGTGCAAAAGCCCGTCAGTGAGAGTGAATTGGGATGGCGTTCATTATACTGAGGCAGCTAACAAGTTTGTTTTTGACAAAATTTCAACTGGAGCATTTTCAGATCCACCCCTTCCCTTAAAACATGCATGTCACAGGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005488 GO:0006950 GO:0006952 GO:0008150 GO:0016787 GO:0016788 GO:0019863 GO:0019865 GO:0044877 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

182

Amino Acids

20.21

Weight (kDa)

8.84

Isoelectric Point (pI)

33.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 8 - 162 4.7e-23 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 139
AclWI GGATC 4 cut(s) 52, 65, 219, 500
AcsI RAATTY 1 cut(s) 483
AcuI CTGAAG 1 cut(s) 203
AfaI GTAC 1 cut(s) 335
AfiI CCNNNNNNNGG 1 cut(s) 184
AgsI TTSAA 3 cut(s) 23, 200, 489
AluBI AGCT 3 cut(s) 101, 116, 461
AluI AGCT 3 cut(s) 101, 116, 461
Alw26I GTCTC 1 cut(s) 89
AlwI GGATC 4 cut(s) 52, 65, 219, 500
AoxI GGCC 1 cut(s) 71
ApeKI GCWGC 3 cut(s) 135, 224, 458
ApoI RAATTY 1 cut(s) 483
AspLEI GCGC 1 cut(s) 140
AspS9I GGNCC 1 cut(s) 71
BamHI GGATCC 1 cut(s) 57
BbvI GCAGC 3 cut(s) 122, 211, 470
BccI CCATC 2 cut(s) 118, 431
BcoDI GTCTC 1 cut(s) 89
BfmI CTRYAG 1 cut(s) 340
BisI GCNGC 3 cut(s) 136, 225, 459
BlsI GCNGC 3 cut(s) 137, 226, 460
BmgT120I GGNCC 1 cut(s) 71
BmiI GGNNCC 3 cut(s) 43, 59, 276
BmrI ACTGGG 1 cut(s) 78
BmuI ACTGGG 1 cut(s) 78
BpmI CTGGAG 1 cut(s) 513
BsaAI YACGTR 1 cut(s) 238
Bsc4I CCNNNNNNNGG 1 cut(s) 184
Bse1I ACTGG 3 cut(s) 13, 73, 496
BseGI GGATG 2 cut(s) 362, 442
BseLI CCNNNNNNNGG 1 cut(s) 184
BseMII CTCAG 2 cut(s) 176, 444
BseNI ACTGG 3 cut(s) 13, 73, 496
BseXI GCAGC 3 cut(s) 122, 211, 470
BshFI GGCC 1 cut(s) 73
BslI CCNNNNNNNGG 1 cut(s) 184
BsmAI GTCTC 1 cut(s) 89
BsnI GGCC 1 cut(s) 73
Bsp143I GATC 4 cut(s) 47, 57, 211, 505
BspANI GGCC 1 cut(s) 73
BspCNI CTCAG 2 cut(s) 175, 445
BspLI GGNNCC 3 cut(s) 43, 59, 276
BspPI GGATC 4 cut(s) 52, 65, 219, 500
BsrI ACTGG 3 cut(s) 13, 73, 496
BssMI GATC 4 cut(s) 47, 57, 211, 505
Bst4CI ACNGT 3 cut(s) 196, 370, 376
BstBAI YACGTR 1 cut(s) 238
BstDEI CTNAG 3 cut(s) 162, 203, 453
BstF5I GGATG 2 cut(s) 362, 442
BstHHI GCGC 1 cut(s) 140
BstKTI GATC 4 cut(s) 50, 60, 214, 508
BstMAI GTCTC 1 cut(s) 89
BstMBI GATC 4 cut(s) 47, 57, 211, 505
BstNSI RCATGY 2 cut(s) 530, 534
BstSFI CTRYAG 1 cut(s) 340
BstV1I GCAGC 3 cut(s) 122, 211, 470
BstX2I RGATCY 3 cut(s) 57, 211, 505
BstXI CCANNNNNNTGG 1 cut(s) 68
BstYI RGATCY 3 cut(s) 57, 211, 505
BsuRI GGCC 1 cut(s) 73
BtsCI GGATG 2 cut(s) 362, 442
BtsIMutI CAGTG 2 cut(s) 66, 426
CfoI GCGC 1 cut(s) 140
Cfr13I GGNCC 1 cut(s) 71
Csp6I GTAC 1 cut(s) 334
CviAII CATG 2 cut(s) 527, 531
CviQI GTAC 1 cut(s) 334
DdeI CTNAG 3 cut(s) 162, 203, 453
DpnI GATC 4 cut(s) 49, 59, 213, 507
DpnII GATC 4 cut(s) 47, 57, 211, 505
Eco57I CTGAAG 1 cut(s) 203
EcoT22I ATGCAT 1 cut(s) 532
FaeI CATG 2 cut(s) 530, 534
FaiI YATR 9 cut(s) 31, 93, 245, 247, 288, 342, 450, 528, 532
FatI CATG 2 cut(s) 526, 530
Fnu4HI GCNGC 3 cut(s) 136, 225, 459
FokI GGATG 2 cut(s) 369, 449
Fsp4HI GCNGC 3 cut(s) 136, 225, 459
FspI TGCGCA 1 cut(s) 139
GlaI GCGC 1 cut(s) 139
GluI GCNGC 3 cut(s) 136, 225, 459
GsuI CTGGAG 1 cut(s) 513
HaeIII GGCC 1 cut(s) 73
HhaI GCGC 1 cut(s) 140
Hin1II CATG 2 cut(s) 530, 534
Hin6I GCGC 1 cut(s) 138
HinP1I GCGC 1 cut(s) 138
Hpy166II GTNNAC 1 cut(s) 241
Hpy188I TCNGA 1 cut(s) 505
Hpy8I GTNNAC 1 cut(s) 241
HpyAV CCTTC 3 cut(s) 178, 202, 524
HpyCH4III ACNGT 3 cut(s) 196, 370, 376
HpyCH4IV ACGT 1 cut(s) 237
HpyCH4V TGCA 3 cut(s) 227, 408, 530
HpyF3I CTNAG 3 cut(s) 162, 203, 453
HpySE526I ACGT 1 cut(s) 237
Hsp92II CATG 2 cut(s) 530, 534
HspAI GCGC 1 cut(s) 138
Kzo9I GATC 4 cut(s) 47, 57, 211, 505
LmnI GCTCC 3 cut(s) 41, 274, 494
LpnPI CCDG 6 cut(s) 26, 54, 58, 339, 477, 523
Lsp1109I GCAGC 3 cut(s) 122, 211, 470
MaeII ACGT 1 cut(s) 237
MaeIII GTNAC 2 cut(s) 370, 533
MalI GATC 4 cut(s) 49, 59, 213, 507
MboI GATC 4 cut(s) 47, 57, 211, 505
MboII GAAGA 1 cut(s) 206
MfeI CAATTG 1 cut(s) 75
MflI RGATCY 3 cut(s) 57, 211, 505
MluCI AATT 4 cut(s) 75, 103, 430, 483
MnlI CCTC 2 cut(s) 121, 448
Mph1103I ATGCAT 1 cut(s) 532
MseI TTAA 2 cut(s) 171, 521
MunI CAATTG 1 cut(s) 75
NdeII GATC 4 cut(s) 47, 57, 211, 505
NlaIII CATG 2 cut(s) 530, 534
NlaIV GGNNCC 3 cut(s) 43, 59, 276
NmuCI GTSAC 1 cut(s) 533
NsbI TGCGCA 1 cut(s) 139
NsiI ATGCAT 1 cut(s) 532
NspI RCATGY 2 cut(s) 530, 534
PkrI GCNGC 3 cut(s) 137, 226, 460
Ppu21I YACGTR 1 cut(s) 238
PspN4I GGNNCC 3 cut(s) 43, 59, 276
PspPI GGNCC 1 cut(s) 71
PsrI GAACNNNNNNTAC 2 cut(s) 355, 387
PsuI RGATCY 3 cut(s) 57, 211, 505
RsaI GTAC 1 cut(s) 335
RsaNI GTAC 1 cut(s) 334
SaqAI TTAA 2 cut(s) 171, 521
SatI GCNGC 3 cut(s) 136, 225, 459
Sau3AI GATC 4 cut(s) 47, 57, 211, 505
Sau96I GGNCC 1 cut(s) 71
SetI ASST 5 cut(s) 103, 118, 132, 240, 463
SfcI CTRYAG 1 cut(s) 340
Sse9I AATT 4 cut(s) 75, 103, 430, 483
SspI AATATT 1 cut(s) 393
TaaI ACNGT 3 cut(s) 196, 370, 376
TaiI ACGT 1 cut(s) 240
TasI AATT 4 cut(s) 75, 103, 430, 483
TatI WGTACW 1 cut(s) 333
Tru1I TTAA 2 cut(s) 171, 521
Tru9I TTAA 2 cut(s) 171, 521
TscAI CASTG 2 cut(s) 73, 426
TseFI GTSAC 1 cut(s) 533
TseI GCWGC 3 cut(s) 135, 224, 458
Tsp45I GTSAC 1 cut(s) 533
TspDTI ATGAA 2 cut(s) 168, 434
TspRI CASTG 2 cut(s) 73, 426
XapI RAATTY 1 cut(s) 483
XceI RCATGY 2 cut(s) 530, 534
XcmI CCANNNNNNNNNTGG 1 cut(s) 395
Zsp2I ATGCAT 1 cut(s) 532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.