Rh6AG367400

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0000978
Physical Location & Seq
Reverse (-)
56581215 .. 56587700
6486 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG367400.1

Sequence Viewer

Length: 1149 bp
ATGTCTGATGAAGGAAGAGTGCACCCGGATTGCAGGAATGCATCCAATCCCTACCATGAATGCAGCGAGTTTTGCTTCAAAGTTATTGCTGAAACCAAAGCTCGGATGGATCAAAATCAATCAGAATCTGTACAAGTTAGTGGTGGTAGTGGCAAATCAAAATTAGAGGCTGCTCAGCAAGAGGAAGATCTAGATAGACCATCAGAGACTGATGAACATGCTGGTGATGGTGATGAGCCTCCTGTTGACAAGGAGGAGAATATGGAAGGAGCCTTCACGAAGTTCACAGGGAGGAAGAAAAAGTTGTGGGAATTAAGGATGAAGATGATGATTCCTGGTATAGTTTTGGTTGATGCTTTTGCTTTTGGTCAGATGCTTGGATTCTACAATAGTACCTTGGCTTTGAAAGACTGCGAGTTTCCAGCCATCTTCAACTTCGGAGACTCAAATTCAGATACTGGTGGATTAGCCGCATCCCTTCTATTAACACCAACCACACCTTATGGAGAGACCTATTTCCATATGCGGGTCGGAAGATTCTCTGATGGAAGACTCATTATCGGCTTCCTTGGGGAAGTATTTGCAAGTCTAATGCCCAAGGAGGAGTACTTCTCTAAAGCATTATACACATTCGACATTGGTCAGAATGATCTTAGCGAGGGATTTACCCGCGGTAACATGACTATACAGGAAGTCAATGCATCTGTAACTGATATAATCACTGGTTTCTCAACTTATATCAAGAAAATATATGATTTGGGAGCAAGATCAATATGGATCCACAATACGGGGCCGATTGGCTGTCTCCCTTACATTGTGGCAGACTTCCCATCAGCTCAGAAAGATGAGATTGGCTGTGCAAAAGCTTACAATGAAGTAGCTCAACATTTTAACCACGAGCTGAAGCTGGCCATAGTTCAACTCAGGAAGGATTTTCCTTTGGCTGCATTCACTTATGTAGATGTGTATTATGTCGTCAAATACTCTCTTTTCAAGGAACCTGAAAAGTATGGATTTGAGCTGCCACTTGTTGCTTGCTGCGGTTATGGTGGTAAGTACAACTATAACAATACTGCTCGATGTGGAGCAACAATTACAGTCAATGGAAGCCAAATATTTGTTGGTTCATGCAAAACTCAACTAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005488 GO:0006950 GO:0006952 GO:0008150 GO:0016787 GO:0016788 GO:0019863 GO:0019865 GO:0044877 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

382

Amino Acids

42.5

Weight (kDa)

5.07

Isoelectric Point (pI)

27.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 143 - 351 1.2e-24 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 672
AciI CCGC 5 cut(s) 471, 526, 670, 672, 1041
AclWI GGATC 3 cut(s) 117, 772, 785
AcoI YGGCCR 1 cut(s) 909
AcsI RAATTY 1 cut(s) 448
AcuI CTGAAG 1 cut(s) 923
AfaI GTAC 4 cut(s) 132, 394, 608, 1058
AfiI CCNNNNNNNGG 3 cut(s) 102, 526, 787
AgsI TTSAA 5 cut(s) 79, 406, 433, 920, 994
AjnI CCWGG 1 cut(s) 334
AluBI AGCT 7 cut(s) 101, 836, 866, 881, 901, 907, 1021
AluI AGCT 7 cut(s) 101, 836, 866, 881, 901, 907, 1021
Alw21I GWGCWC 1 cut(s) 24
Alw26I GTCTC 4 cut(s) 200, 435, 503, 809
Alw44I GTGCAC 1 cut(s) 20
AlwI GGATC 3 cut(s) 117, 772, 785
AlwNI CAGNNNCTG 3 cut(s) 128, 209, 458
AoxI GGCC 2 cut(s) 791, 909
ApaLI GTGCAC 1 cut(s) 20
ApeKI GCWGC 5 cut(s) 63, 170, 944, 1021, 1038
ApoI RAATTY 1 cut(s) 448
AspS9I GGNCC 1 cut(s) 791
AsuC2I CCSGG 1 cut(s) 26
AsuHPI GGTGA 2 cut(s) 236, 242
BaeGI GKGCMC 1 cut(s) 24
BaeI ACNNNNGTAYC 2 cut(s) 376, 409
BalI TGGCCA 1 cut(s) 911
BamHI GGATCC 1 cut(s) 777
BauI CACGAG 1 cut(s) 896
BbsI GAAGAC 1 cut(s) 556
Bbv12I GWGCWC 1 cut(s) 24
BbvI GCAGC 5 cut(s) 75, 157, 931, 1008, 1025
BccI CCATC 6 cut(s) 100, 208, 221, 434, 539, 838
BciT130I CCWGG 1 cut(s) 336
BcnI CCSGG 1 cut(s) 26
BcoDI GTCTC 4 cut(s) 200, 435, 503, 809
BfaI CTAG 2 cut(s) 191, 1142
BglII AGATCT 1 cut(s) 187
BisI GCNGC 6 cut(s) 64, 171, 471, 945, 1022, 1039
BlpI GCTNAGC 1 cut(s) 174
BlsI GCNGC 6 cut(s) 65, 172, 472, 946, 1023, 1040
BmcAI AGTACT 1 cut(s) 608
Bme1390I CCNGG 2 cut(s) 26, 336
BmgT120I GGNCC 1 cut(s) 791
BmiI GGNNCC 4 cut(s) 271, 779, 792, 999
BmrFI CCNGG 2 cut(s) 26, 336
BmsI GCATC 5 cut(s) 50, 343, 363, 482, 710
BoxI GACNNNNGTC 1 cut(s) 639
BpiI GAAGAC 1 cut(s) 556
BplI GAGNNNNNCTC 2 cut(s) 596, 628
Bpu1102I GCTNAGC 1 cut(s) 174
BpuMI CCSGG 1 cut(s) 26
BsaBI GATNNNNATC 1 cut(s) 114
BsaI GGTCTC 1 cut(s) 503
BsaJI CCNNGG 4 cut(s) 396, 568, 597, 670
Bsc4I CCNNNNNNNGG 3 cut(s) 102, 526, 787
Bse1I ACTGG 2 cut(s) 463, 727
Bse8I GATNNNNATC 1 cut(s) 114
BseBI CCWGG 1 cut(s) 336
BseDI CCNNGG 4 cut(s) 396, 568, 597, 670
BseGI GGATG 4 cut(s) 41, 111, 324, 473
BseJI GATNNNNATC 1 cut(s) 114
BseLI CCNNNNNNNGG 3 cut(s) 102, 526, 787
BseMII CTCAG 3 cut(s) 188, 851, 937
BseNI ACTGG 2 cut(s) 463, 727
BseRI GAGGAG 2 cut(s) 269, 617
BseSI GKGCMC 1 cut(s) 24
BseXI GCAGC 5 cut(s) 75, 157, 931, 1008, 1025
Bsh1236I CGCG 1 cut(s) 672
BshFI GGCC 2 cut(s) 793, 911
BsiHKAI GWGCWC 1 cut(s) 24
BsiSI CCGG 1 cut(s) 26
BslI CCNNNNNNNGG 3 cut(s) 102, 526, 787
BsmAI GTCTC 4 cut(s) 200, 435, 503, 809
BsmI GAATGC 3 cut(s) 43, 65, 947
BsnI GGCC 2 cut(s) 793, 911
Bso31I GGTCTC 1 cut(s) 503
Bsp1286I GDGCHC 1 cut(s) 24
Bsp1407I TGTACA 1 cut(s) 130
Bsp143I GATC 5 cut(s) 109, 187, 649, 767, 777
Bsp1720I GCTNAGC 1 cut(s) 174
BspACI CCGC 5 cut(s) 471, 526, 670, 672, 1041
BspANI GGCC 2 cut(s) 793, 911
BspCNI CTCAG 3 cut(s) 187, 850, 936
BspFNI CGCG 1 cut(s) 672
BspLI GGNNCC 4 cut(s) 271, 779, 792, 999
BspPI GGATC 3 cut(s) 117, 772, 785
BspTNI GGTCTC 1 cut(s) 503
BsrGI TGTACA 1 cut(s) 130
BsrI ACTGG 2 cut(s) 463, 727
BssECI CCNNGG 4 cut(s) 396, 568, 597, 670
BssMI GATC 5 cut(s) 109, 187, 649, 767, 777
BssSI CACGAG 1 cut(s) 896
BssT1I CCWWGG 3 cut(s) 396, 568, 597
Bst2BI CACGAG 1 cut(s) 896
Bst2UI CCWGG 1 cut(s) 336
Bst4CI ACNGT 1 cut(s) 1099
Bst6I CTCTTC 1 cut(s) 10
BstAUI TGTACA 1 cut(s) 130
BstC8I GCNNGC 2 cut(s) 909, 1036
BstDEI CTNAG 4 cut(s) 174, 653, 837, 923
BstDSI CCRYGG 1 cut(s) 670
BstF5I GGATG 4 cut(s) 41, 111, 324, 473
BstFNI CGCG 1 cut(s) 672
BstKTI GATC 5 cut(s) 112, 190, 652, 770, 780
BstMAI GTCTC 4 cut(s) 200, 435, 503, 809
BstMBI GATC 5 cut(s) 109, 187, 649, 767, 777
BstMWI GCNNNNNNNGC 1 cut(s) 72
BstNI CCWGG 1 cut(s) 336
BstNSI RCATGY 1 cut(s) 221
BstPAI GACNNNNGTC 1 cut(s) 639
BstSCI CCNGG 2 cut(s) 24, 334
BstSLI GKGCMC 1 cut(s) 24
BstUI CGCG 1 cut(s) 672
BstV1I GCAGC 5 cut(s) 75, 157, 931, 1008, 1025
BstV2I GAAGAC 1 cut(s) 556
BstX2I RGATCY 2 cut(s) 187, 777
BstYI RGATCY 2 cut(s) 187, 777
BsuRI GGCC 2 cut(s) 793, 911
BtgI CCRYGG 1 cut(s) 670
BtsCI GGATG 4 cut(s) 41, 111, 324, 473
BtsIMutI CAGTG 1 cut(s) 720
Cac8I GCNNGC 2 cut(s) 909, 1036
CaiI CAGNNNCTG 3 cut(s) 128, 209, 458
Cfr13I GGNCC 1 cut(s) 791
Cfr42I CCGCGG 1 cut(s) 673
Csp6I GTAC 4 cut(s) 131, 393, 607, 1057
CviAII CATG 4 cut(s) 56, 218, 679, 1128
CviQI GTAC 4 cut(s) 131, 393, 607, 1057
DdeI CTNAG 4 cut(s) 174, 653, 837, 923
DpnI GATC 5 cut(s) 111, 189, 651, 769, 779
DpnII GATC 5 cut(s) 109, 187, 649, 767, 777
EaeI YGGCCR 1 cut(s) 909
Eam1104I CTCTTC 1 cut(s) 10
EarI CTCTTC 1 cut(s) 10
Eco130I CCWWGG 3 cut(s) 396, 568, 597
Eco31I GGTCTC 1 cut(s) 503
Eco57I CTGAAG 1 cut(s) 923
EcoRII CCWGG 1 cut(s) 334
EcoT14I CCWWGG 3 cut(s) 396, 568, 597
EcoT22I ATGCAT 2 cut(s) 43, 703
ErhI CCWWGG 3 cut(s) 396, 568, 597
FaeI CATG 4 cut(s) 59, 221, 682, 1131
FatI CATG 4 cut(s) 55, 217, 678, 1127
FauI CCCGC 2 cut(s) 519, 677
FauNDI CATATG 1 cut(s) 522
Fnu4HI GCNGC 6 cut(s) 64, 171, 471, 945, 1022, 1039
FokI GGATG 4 cut(s) 28, 118, 331, 460
Fsp4HI GCNGC 6 cut(s) 64, 171, 471, 945, 1022, 1039
FspBI CTAG 2 cut(s) 191, 1142
GluI GCNGC 6 cut(s) 64, 171, 471, 945, 1022, 1039
HaeIII GGCC 2 cut(s) 793, 911
HapII CCGG 1 cut(s) 26
Hin1II CATG 4 cut(s) 59, 221, 682, 1131
HincII GTYRAC 1 cut(s) 247
HindII GTYRAC 1 cut(s) 247
HindIII AAGCTT 1 cut(s) 864
HinfI GANTC 6 cut(s) 125, 331, 381, 443, 537, 552
HpaII CCGG 1 cut(s) 26
HphI GGTGA 2 cut(s) 236, 242
Hpy166II GTNNAC 3 cut(s) 22, 247, 285
Hpy188III TCNNGA 4 cut(s) 191, 277, 742, 925
Hpy8I GTNNAC 3 cut(s) 22, 247, 285
HpyAV CCTTC 5 cut(s) 5, 260, 283, 488, 922
HpyCH4III ACNGT 1 cut(s) 1099
HpyCH4V TGCA 9 cut(s) 22, 33, 41, 63, 584, 701, 860, 947, 1131
HpyF10VI GCNNNNNNNGC 1 cut(s) 72
HpyF3I CTNAG 4 cut(s) 174, 653, 837, 923
Hsp92II CATG 4 cut(s) 59, 221, 682, 1131
KspI CCGCGG 1 cut(s) 673
Kzo9I GATC 5 cut(s) 109, 187, 649, 767, 777
LmnI GCTCC 3 cut(s) 269, 761, 1085
Lsp1109I GCAGC 5 cut(s) 75, 157, 931, 1008, 1025
LweI GCATC 5 cut(s) 50, 343, 363, 482, 710
MaeI CTAG 2 cut(s) 191, 1142
MaeIII GTNAC 2 cut(s) 674, 706
MalI GATC 5 cut(s) 111, 189, 651, 769, 779
MboI GATC 5 cut(s) 109, 187, 649, 767, 777
MboII GAAGA 7 cut(s) 27, 197, 307, 334, 421, 546, 561
MflI RGATCY 2 cut(s) 187, 777
MhlI GDGCHC 1 cut(s) 24
MlsI TGGCCA 1 cut(s) 911
MluCI AATT 4 cut(s) 161, 311, 448, 1092
MluNI TGGCCA 1 cut(s) 911
MlyI GAGTC 2 cut(s) 437, 546
MmeI TCCRAC 1 cut(s) 511
MnlI CCTC 7 cut(s) 160, 175, 247, 249, 285, 595, 652
Mox20I TGGCCA 1 cut(s) 911
Mph1103I ATGCAT 2 cut(s) 43, 703
MscI TGGCCA 1 cut(s) 911
MseI TTAA 3 cut(s) 314, 485, 891
MslI CAYNNNNRTG 1 cut(s) 222
Msp20I TGGCCA 1 cut(s) 911
MspA1I CMGCKG 1 cut(s) 672
MspI CCGG 1 cut(s) 26
MspR9I CCNGG 2 cut(s) 26, 336
Mva1269I GAATGC 3 cut(s) 43, 65, 947
MvaI CCWGG 1 cut(s) 336
MvnI CGCG 1 cut(s) 672
MwoI GCNNNNNNNGC 1 cut(s) 72
NciI CCSGG 1 cut(s) 26
NdeI CATATG 1 cut(s) 522
NdeII GATC 5 cut(s) 109, 187, 649, 767, 777
NlaIII CATG 4 cut(s) 59, 221, 682, 1131
NlaIV GGNNCC 4 cut(s) 271, 779, 792, 999
NsiI ATGCAT 2 cut(s) 43, 703
NspI RCATGY 1 cut(s) 221
PctI GAATGC 3 cut(s) 43, 65, 947
PfeI GAWTC 4 cut(s) 125, 331, 381, 537
PkrI GCNGC 6 cut(s) 65, 172, 472, 946, 1023, 1040
PleI GAGTC 2 cut(s) 437, 546
PpsI GAGTC 2 cut(s) 437, 546
PshAI GACNNNNGTC 1 cut(s) 639
Psp6I CCWGG 1 cut(s) 334
PspGI CCWGG 1 cut(s) 334
PspN4I GGNNCC 4 cut(s) 271, 779, 792, 999
PspPI GGNCC 1 cut(s) 791
PstNI CAGNNNCTG 3 cut(s) 128, 209, 458
PsuI RGATCY 2 cut(s) 187, 777
RsaI GTAC 4 cut(s) 132, 394, 608, 1058
RsaNI GTAC 4 cut(s) 131, 393, 607, 1057
RseI CAYNNNNRTG 1 cut(s) 222
SacII CCGCGG 1 cut(s) 673
SaqAI TTAA 3 cut(s) 314, 485, 891
SatI GCNGC 6 cut(s) 64, 171, 471, 945, 1022, 1039
Sau3AI GATC 5 cut(s) 109, 187, 649, 767, 777
Sau96I GGNCC 1 cut(s) 791
ScaI AGTACT 1 cut(s) 608
SchI GAGTC 2 cut(s) 437, 546
ScrFI CCNGG 2 cut(s) 26, 336
SduI GDGCHC 1 cut(s) 24
SfaNI GCATC 5 cut(s) 50, 343, 363, 482, 710
Sfr303I CCGCGG 1 cut(s) 673
SgrBI CCGCGG 1 cut(s) 673
SmiMI CAYNNNNRTG 1 cut(s) 222
Sse9I AATT 4 cut(s) 161, 311, 448, 1092
SsiI CCGC 5 cut(s) 471, 526, 670, 672, 1041
SspI AATATT 1 cut(s) 1116
SspMI CTAG 2 cut(s) 191, 1142
StyD4I CCNGG 2 cut(s) 24, 334
StyI CCWWGG 3 cut(s) 396, 568, 597
TaaI ACNGT 1 cut(s) 1099
TaqI TCGA 2 cut(s) 633, 1078
TasI AATT 4 cut(s) 161, 311, 448, 1092
TatI WGTACW 3 cut(s) 130, 606, 1056
TauI GCSGC 1 cut(s) 473
TfiI GAWTC 4 cut(s) 125, 331, 381, 537
Tru1I TTAA 3 cut(s) 314, 485, 891
Tru9I TTAA 3 cut(s) 314, 485, 891
TscAI CASTG 1 cut(s) 727
TseI GCWGC 5 cut(s) 63, 170, 944, 1021, 1038
TspDTI ATGAA 6 cut(s) 24, 72, 228, 335, 888, 1116
TspRI CASTG 1 cut(s) 727
VneI GTGCAC 1 cut(s) 20
XapI RAATTY 1 cut(s) 448
XbaI TCTAGA 1 cut(s) 190
XceI RCATGY 1 cut(s) 221
XcmI CCANNNNNNNNNTGG 2 cut(s) 103, 1118
XspI CTAG 2 cut(s) 191, 1142
ZrmI AGTACT 1 cut(s) 608
Zsp2I ATGCAT 2 cut(s) 43, 703
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.