Rw6G032060

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000978
Physical Location & Seq
Reverse (-)
56161723 .. 56164348
2626 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G032060.1

Sequence Viewer

Length: 1176 bp
ATGGAGTACACTCCTAGAGTTCTTATTTCTCTGTTCTGCTGCTATATGCTCCTATTGCATATTACCAGTATTACTGTAAGCCCTGCCTTGGCTTTGGAAGACTGCGAGTTTCCAGCCATCTTCAACTTCGGAGACTCAAATTCGGATACTGGTGGATTCGCAGCATCCATTTATCCCCCACCTACACCTTATGGAGAGACCTATTTTCATATGCCGGTTGGAAGAATCTCAGATGGAAGACTCATTGTCGATTTCCTCGCAAAGAGTCTTGGTCATCCTTATCTAAGCGCATATCTAGATTCCCTCGGGACCAATTTCTCGCATGGTGCAAATTTTGCAACTTCAGCTTCCACTATCAGACTCCCAGATCTCATTTTACCAGCCGGCGGATTTAGTCCCTTCTACCTTGATATTCAATACGTACAGTTCCGGGAACTAAAGTCCAGATCACAACTCATAAGGCAACGAGGGGGAATATTTGCAAGTTTAATGCCCAAGGAGGAGTACTTCTCCAAAGCTTTATACACATTCGACATTGGTCAGAATGATCTTGGCGAGGGATTTTTCGGTAACAAGACTATACAGGAAGTCAATGCATCTGTTCCTGATATAATCACTGGTTTCTCAACGAATATCAAGAAAATATATGATTTGGGGGCAAGATCATTTTGGATCCACAATACGGGGCCGATTGGCTGTGTCCCTTACATTTTGGTGAAATTCCCATCAGCTCAGAAAGATGAGGTTGGCTGTGCAAAGGCTTACAATGAGGTAGCTCAACATTTTAACCACGAGCTGAAGCAGGCCATAGTTCAACTCAGGAAGGATCTTCCTTTGGCTGCATTCACTTATGTAGATGTGTATTCCGTCAAATATTCTATTTTCAAGGAACCCAAAAAATATGGATTTGAGCTCCCACTTGTTGCTTGTTGTGGCTATGGTGGTAAGTACAACTATAACAGTAGTACAGGATGTGGAGCAACAATCACAGTCAATGGAAGTCAAATCTTTGTTGGCTCATGCAAGAACCCATCAGCTAGAGTGAACTGGGATGGGATTCATTATACTGAGGCAGCTGCGAAATTTGTTTTTGAGAAAATCTCAACTGGAGCATATTCAGATCCACCTCTGCCATTGAAACAAGCATGCCACAGGAGTGGAAATAAGAAAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005488 GO:0006950 GO:0006952 GO:0008150 GO:0016787 GO:0016788 GO:0019863 GO:0019865 GO:0044877 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

391

Amino Acids

43.17

Weight (kDa)

8.2

Isoelectric Point (pI)

36.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 40 - 367 9.9e-46 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 387
AclWI GGATC 4 cut(s) 667, 680, 834, 1115
AcsI RAATTY 4 cut(s) 139, 331, 719, 1082
AcuI CTGAAG 2 cut(s) 327, 818
AfaI GTAC 5 cut(s) 8, 423, 506, 950, 967
AfiI CCNNNNNNNGG 3 cut(s) 88, 386, 682
AgsI TTSAA 5 cut(s) 124, 416, 815, 886, 1138
AhdI GACNNNNNGTC 1 cut(s) 245
AleI CACNNNNGTG 1 cut(s) 1155
AluBI AGCT 8 cut(s) 347, 518, 731, 776, 796, 913, 1037, 1076
AluI AGCT 8 cut(s) 347, 518, 731, 776, 796, 913, 1037, 1076
Alw21I GWGCWC 1 cut(s) 915
Alw26I GTCTC 2 cut(s) 126, 191
AlwI GGATC 4 cut(s) 667, 680, 834, 1115
Ama87I CYCGRG 1 cut(s) 305
AoxI GGCC 2 cut(s) 686, 804
ApeKI GCWGC 5 cut(s) 39, 161, 839, 1073, 1076
ApoI RAATTY 4 cut(s) 139, 331, 719, 1082
AspLEI GCGC 1 cut(s) 290
AspS9I GGNCC 2 cut(s) 309, 686
AsuC2I CCSGG 1 cut(s) 431
AsuHPI GGTGA 1 cut(s) 727
AvaI CYCGRG 1 cut(s) 305
AvaII GGWCC 1 cut(s) 309
BamHI GGATCC 1 cut(s) 672
BanII GRGCYC 1 cut(s) 915
BauI CACGAG 1 cut(s) 791
BbsI GAAGAC 2 cut(s) 105, 244
Bbv12I GWGCWC 1 cut(s) 915
BbvI GCAGC 5 cut(s) 26, 173, 826, 1063, 1085
BccI CCATC 5 cut(s) 125, 227, 733, 1039, 1046
BciVI GTATCC 1 cut(s) 139
BcnI CCSGG 1 cut(s) 431
BcoDI GTCTC 2 cut(s) 126, 191
BfaI CTAG 3 cut(s) 15, 296, 1038
BfuI GTATCC 1 cut(s) 139
BglII AGATCT 1 cut(s) 367
BisI GCNGC 5 cut(s) 40, 162, 840, 1074, 1077
BlsI GCNGC 5 cut(s) 41, 163, 841, 1075, 1078
BmcAI AGTACT 1 cut(s) 506
Bme1390I CCNGG 1 cut(s) 431
Bme18I GGWCC 1 cut(s) 309
BmeRI GACNNNNNGTC 1 cut(s) 245
BmeT110I CYCGRG 1 cut(s) 305
BmgT120I GGNCC 2 cut(s) 309, 686
BmiI GGNNCC 4 cut(s) 310, 674, 687, 891
BmrFI CCNGG 1 cut(s) 431
BmrI ACTGGG 1 cut(s) 1057
BmsI GCATC 2 cut(s) 173, 605
BmuI ACTGGG 1 cut(s) 1057
BoxI GACNNNNGTC 1 cut(s) 537
BpiI GAAGAC 2 cut(s) 105, 244
BplI GAGNNNNNCTC 4 cut(s) 494, 526, 1085, 1117
BpmI CTGGAG 1 cut(s) 1128
BpuMI CCSGG 1 cut(s) 431
BsaAI YACGTR 1 cut(s) 421
BsaI GGTCTC 1 cut(s) 191
BsaJI CCNNGG 3 cut(s) 87, 304, 495
Bsc4I CCNNNNNNNGG 3 cut(s) 88, 386, 682
Bse118I RCCGGY 2 cut(s) 214, 383
Bse1I ACTGG 5 cut(s) 66, 154, 622, 1052, 1111
BseDI CCNNGG 3 cut(s) 87, 304, 495
BseGI GGATG 4 cut(s) 164, 274, 977, 1057
BseLI CCNNNNNNNGG 3 cut(s) 88, 386, 682
BseMII CTCAG 4 cut(s) 243, 746, 832, 1059
BseNI ACTGG 5 cut(s) 66, 154, 622, 1052, 1111
BseRI GAGGAG 1 cut(s) 515
BseXI GCAGC 5 cut(s) 26, 173, 826, 1063, 1085
BshFI GGCC 2 cut(s) 688, 806
BsiHKAI GWGCWC 1 cut(s) 915
BsiHKCI CYCGRG 1 cut(s) 305
BsiSI CCGG 3 cut(s) 215, 384, 430
BslFI GGGAC 3 cut(s) 322, 381, 686
BslI CCNNNNNNNGG 3 cut(s) 88, 386, 682
BsmAI GTCTC 2 cut(s) 126, 191
BsmFI GGGAC 3 cut(s) 322, 381, 686
BsmI GAATGC 1 cut(s) 842
BsnI GGCC 2 cut(s) 688, 806
Bso31I GGTCTC 1 cut(s) 191
BsoBI CYCGRG 1 cut(s) 305
Bsp1286I GDGCHC 1 cut(s) 915
Bsp143I GATC 7 cut(s) 367, 446, 547, 662, 672, 826, 1120
BspACI CCGC 1 cut(s) 387
BspANI GGCC 2 cut(s) 688, 806
BspCNI CTCAG 4 cut(s) 242, 745, 831, 1060
BspLI GGNNCC 4 cut(s) 310, 674, 687, 891
BspPI GGATC 4 cut(s) 667, 680, 834, 1115
BspTNI GGTCTC 1 cut(s) 191
BsrFI RCCGGY 2 cut(s) 214, 383
BsrI ACTGG 5 cut(s) 66, 154, 622, 1052, 1111
BssAI RCCGGY 2 cut(s) 214, 383
BssECI CCNNGG 3 cut(s) 87, 304, 495
BssMI GATC 7 cut(s) 367, 446, 547, 662, 672, 826, 1120
BssSI CACGAG 1 cut(s) 791
BssT1I CCWWGG 2 cut(s) 87, 495
Bst2BI CACGAG 1 cut(s) 791
Bst4CI ACNGT 4 cut(s) 76, 426, 962, 991
BstAPI GCANNNNNTGC 1 cut(s) 335
BstBAI YACGTR 1 cut(s) 421
BstC8I GCNNGC 3 cut(s) 385, 804, 1147
BstDEI CTNAG 5 cut(s) 229, 284, 732, 818, 1068
BstF5I GGATG 4 cut(s) 164, 274, 977, 1057
BstHHI GCGC 1 cut(s) 290
BstKTI GATC 7 cut(s) 370, 449, 550, 665, 675, 829, 1123
BstMAI GTCTC 2 cut(s) 126, 191
BstMBI GATC 7 cut(s) 367, 446, 547, 662, 672, 826, 1120
BstMWI GCNNNNNNNGC 3 cut(s) 55, 335, 344
BstNSI RCATGY 1 cut(s) 1149
BstPAI GACNNNNGTC 1 cut(s) 537
BstSCI CCNGG 1 cut(s) 429
BstSNI TACGTA 1 cut(s) 421
BstV1I GCAGC 5 cut(s) 26, 173, 826, 1063, 1085
BstV2I GAAGAC 2 cut(s) 105, 244
BstX2I RGATCY 4 cut(s) 367, 672, 826, 1120
BstXI CCANNNNNNTGG 1 cut(s) 1157
BstYI RGATCY 4 cut(s) 367, 672, 826, 1120
BsuI GTATCC 1 cut(s) 139
BsuRI GGCC 2 cut(s) 688, 806
BtsCI GGATG 4 cut(s) 164, 274, 977, 1057
BtsIMutI CAGTG 1 cut(s) 615
Cac8I GCNNGC 3 cut(s) 385, 804, 1147
CfoI GCGC 1 cut(s) 290
Cfr10I RCCGGY 2 cut(s) 214, 383
Cfr13I GGNCC 2 cut(s) 309, 686
Csp6I GTAC 5 cut(s) 7, 422, 505, 949, 966
CviAII CATG 3 cut(s) 323, 1020, 1146
CviQI GTAC 5 cut(s) 7, 422, 505, 949, 966
DdeI CTNAG 5 cut(s) 229, 284, 732, 818, 1068
DpnI GATC 7 cut(s) 369, 448, 549, 664, 674, 828, 1122
DpnII GATC 7 cut(s) 367, 446, 547, 662, 672, 826, 1120
DriI GACNNNNNGTC 1 cut(s) 245
Eam1105I GACNNNNNGTC 1 cut(s) 245
EciI GGCGGA 1 cut(s) 402
Ecl136II GAGCTC 1 cut(s) 913
Eco105I TACGTA 1 cut(s) 421
Eco130I CCWWGG 2 cut(s) 87, 495
Eco24I GRGCYC 1 cut(s) 915
Eco31I GGTCTC 1 cut(s) 191
Eco47I GGWCC 1 cut(s) 309
Eco53kI GAGCTC 1 cut(s) 913
Eco57I CTGAAG 2 cut(s) 327, 818
Eco88I CYCGRG 1 cut(s) 305
EcoICRI GAGCTC 1 cut(s) 913
EcoT14I CCWWGG 2 cut(s) 87, 495
EcoT22I ATGCAT 1 cut(s) 598
EcoT38I GRGCYC 1 cut(s) 915
ErhI CCWWGG 2 cut(s) 87, 495
FaeI CATG 3 cut(s) 326, 1023, 1149
FaqI GGGAC 3 cut(s) 322, 381, 686
FatI CATG 3 cut(s) 322, 1019, 1145
FauNDI CATATG 1 cut(s) 210
Fnu4HI GCNGC 5 cut(s) 40, 162, 840, 1074, 1077
FokI GGATG 4 cut(s) 151, 261, 984, 1064
FriOI GRGCYC 1 cut(s) 915
Fsp4HI GCNGC 5 cut(s) 40, 162, 840, 1074, 1077
FspBI CTAG 3 cut(s) 15, 296, 1038
GlaI GCGC 1 cut(s) 289
GluI GCNGC 5 cut(s) 40, 162, 840, 1074, 1077
GsuI CTGGAG 1 cut(s) 1128
HaeIII GGCC 2 cut(s) 688, 806
HapII CCGG 3 cut(s) 215, 384, 430
HhaI GCGC 1 cut(s) 290
Hin1II CATG 3 cut(s) 326, 1023, 1149
Hin6I GCGC 1 cut(s) 288
HinP1I GCGC 1 cut(s) 288
HindIII AAGCTT 1 cut(s) 516
HinfI GANTC 8 cut(s) 134, 156, 225, 240, 265, 299, 360, 1057
HpaII CCGG 3 cut(s) 215, 384, 430
HphI GGTGA 1 cut(s) 727
Hpy166II GTNNAC 2 cut(s) 9, 1045
Hpy188I TCNGA 7 cut(s) 131, 145, 232, 359, 543, 735, 1120
Hpy188III TCNNGA 6 cut(s) 296, 307, 444, 605, 637, 820
Hpy8I GTNNAC 2 cut(s) 9, 1045
HpyAV CCTTC 2 cut(s) 409, 817
HpyCH4III ACNGT 4 cut(s) 76, 426, 962, 991
HpyCH4IV ACGT 1 cut(s) 420
HpyCH4V TGCA 8 cut(s) 58, 329, 338, 482, 596, 755, 842, 1023
HpyF10VI GCNNNNNNNGC 3 cut(s) 55, 335, 344
HpyF3I CTNAG 5 cut(s) 229, 284, 732, 818, 1068
HpySE526I ACGT 1 cut(s) 420
Hsp92II CATG 3 cut(s) 326, 1023, 1149
HspAI GCGC 1 cut(s) 288
KroI GCCGGC 1 cut(s) 383
KroNI GCCGGC 1 cut(s) 385
Kzo9I GATC 7 cut(s) 367, 446, 547, 662, 672, 826, 1120
LmnI GCTCC 4 cut(s) 54, 918, 977, 1109
Lsp1109I GCAGC 5 cut(s) 26, 173, 826, 1063, 1085
LweI GCATC 2 cut(s) 173, 605
MaeI CTAG 3 cut(s) 15, 296, 1038
MaeII ACGT 1 cut(s) 420
MaeIII GTNAC 1 cut(s) 569
MalI GATC 7 cut(s) 369, 448, 549, 664, 674, 828, 1122
MboI GATC 7 cut(s) 367, 446, 547, 662, 672, 826, 1120
MboII GAAGA 5 cut(s) 110, 112, 234, 249, 821
MflI RGATCY 4 cut(s) 367, 672, 826, 1120
MhlI GDGCHC 1 cut(s) 915
MluCI AATT 5 cut(s) 139, 313, 331, 719, 1082
MlyI GAGTC 4 cut(s) 128, 234, 274, 354
MmeI TCCRAC 1 cut(s) 199
MnlI CCTC 9 cut(s) 266, 314, 461, 493, 550, 736, 763, 1063, 1137
Mph1103I ATGCAT 1 cut(s) 598
MroNI GCCGGC 1 cut(s) 383
MseI TTAA 2 cut(s) 488, 786
MslI CAYNNNNRTG 2 cut(s) 713, 1155
MspA1I CMGCKG 1 cut(s) 1076
MspI CCGG 3 cut(s) 215, 384, 430
MspR9I CCNGG 1 cut(s) 431
Mva1269I GAATGC 1 cut(s) 842
MwoI GCNNNNNNNGC 3 cut(s) 55, 335, 344
NaeI GCCGGC 1 cut(s) 385
NciI CCSGG 1 cut(s) 431
NdeI CATATG 1 cut(s) 210
NdeII GATC 7 cut(s) 367, 446, 547, 662, 672, 826, 1120
NgoMIV GCCGGC 1 cut(s) 383
NlaIII CATG 3 cut(s) 326, 1023, 1149
NlaIV GGNNCC 4 cut(s) 310, 674, 687, 891
NsiI ATGCAT 1 cut(s) 598
NspI RCATGY 1 cut(s) 1149
OliI CACNNNNGTG 1 cut(s) 1155
PaeI GCATGC 1 cut(s) 1149
PctI GAATGC 1 cut(s) 842
PdiI GCCGGC 1 cut(s) 385
PfeI GAWTC 4 cut(s) 156, 225, 299, 1057
PfoI TCCNGGA 1 cut(s) 429
PkrI GCNGC 5 cut(s) 41, 163, 841, 1075, 1078
PleI GAGTC 4 cut(s) 128, 234, 273, 354
PpsI GAGTC 4 cut(s) 128, 234, 273, 354
Ppu21I YACGTR 1 cut(s) 421
PshAI GACNNNNGTC 1 cut(s) 537
Psp124BI GAGCTC 1 cut(s) 915
PspN4I GGNNCC 4 cut(s) 310, 674, 687, 891
PspPI GGNCC 2 cut(s) 309, 686
PsuI RGATCY 4 cut(s) 367, 672, 826, 1120
PvuII CAGCTG 1 cut(s) 1076
RsaI GTAC 5 cut(s) 8, 423, 506, 950, 967
RsaNI GTAC 5 cut(s) 7, 422, 505, 949, 966
RseI CAYNNNNRTG 2 cut(s) 713, 1155
SacI GAGCTC 1 cut(s) 915
SaqAI TTAA 2 cut(s) 488, 786
SatI GCNGC 5 cut(s) 40, 162, 840, 1074, 1077
Sau3AI GATC 7 cut(s) 367, 446, 547, 662, 672, 826, 1120
Sau96I GGNCC 2 cut(s) 309, 686
ScaI AGTACT 1 cut(s) 506
SchI GAGTC 4 cut(s) 128, 234, 274, 354
ScrFI CCNGG 1 cut(s) 431
SduI GDGCHC 1 cut(s) 915
SfaNI GCATC 2 cut(s) 173, 605
SinI GGWCC 1 cut(s) 309
SmiMI CAYNNNNRTG 2 cut(s) 713, 1155
SnaBI TACGTA 1 cut(s) 421
SphI GCATGC 1 cut(s) 1149
Sse9I AATT 5 cut(s) 139, 313, 331, 719, 1082
SsiI CCGC 1 cut(s) 387
SspI AATATT 2 cut(s) 477, 875
SspMI CTAG 3 cut(s) 15, 296, 1038
SstI GAGCTC 1 cut(s) 915
StyD4I CCNGG 1 cut(s) 429
StyI CCWWGG 2 cut(s) 87, 495
TaaI ACNGT 4 cut(s) 76, 426, 962, 991
TaiI ACGT 1 cut(s) 423
TaqI TCGA 2 cut(s) 249, 531
TasI AATT 5 cut(s) 139, 313, 331, 719, 1082
TatI WGTACW 4 cut(s) 6, 504, 948, 965
TfiI GAWTC 4 cut(s) 156, 225, 299, 1057
Tru1I TTAA 2 cut(s) 488, 786
Tru9I TTAA 2 cut(s) 488, 786
TscAI CASTG 1 cut(s) 622
TseI GCWGC 5 cut(s) 39, 161, 839, 1073, 1076
TspDTI ATGAA 2 cut(s) 197, 1049
TspGWI ACGGA 1 cut(s) 856
TspRI CASTG 1 cut(s) 622
VpaK11BI GGWCC 1 cut(s) 309
XapI RAATTY 4 cut(s) 139, 331, 719, 1082
XbaI TCTAGA 1 cut(s) 295
XceI RCATGY 1 cut(s) 1149
XspI CTAG 3 cut(s) 15, 296, 1038
ZrmI AGTACT 1 cut(s) 506
Zsp2I ATGCAT 1 cut(s) 598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.