RchiOBHm_Chr6g0295811

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000978
Physical Location & Seq
Reverse (-)
57721604 .. 57724208
2605 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26549

Sequence Viewer

Length: 1158 bp
ATGGGGTACTCTAAAGTTATTATTTCTCTGTTCTGCTCCTATATGCTTATATTGCATATTACCAGTAGTTCTGTGATCCCTACCTTGGCTTTGAAAGACTGTGAGTTTCCTGCCATCTTCAACTTCGGAGACTCAAATTCAGATACTGGTGGACTAGCCGCATCCCTTCTACCACCAACCACACCTTATGGAGAGACCTATTTCCATATGCCGGCCGGAAGATTCTCTGATGGAAGACTCATCATCGATTTCCTTGCAAAGAGTCTTGGTCACCCTTATCTAAGCGCATATCTGGATTCTCTCGGGACAAATTTCTCGTATGGTGCAAATTTTGCAACTGCAGCTTCCACTATCAGACTCCCAGATCTCATTATACCAGCCGGTGGATTTAGTCCCTTCTACCTTGATATTCAATATATGCAGTTCCTGCAACTAAAATCCAGATCACAACTCATAAGGCATCGAGGGGGAATATTTGCAAGTTTAATGCCCAAGGAGGAGTACTTCTCCAAAGCTTTATACACATTCGACATTGGCCAGAATGATCTTGGCGAGGGATTTTTCGGTAACCTGACTATACAGGAAGTAAATGCATCTGTTCCTGATATAATCACTGGTTTCTCAACTAATATCAAGAAAATATATGATTTGGGAGCAAGATCATTATGGATCCACAATACGGGGCCGATTGGCTGTCTCCCTTACATTGTGGCAAACTTCCCGTCAGCTGAGAAAGATGAGATTGGCTGTGCAAAGGCTTACAATGAAGTAGCTAAACATTTTAACCACGAGCTGAAGCAGGCCATAGTTCAACTCAGGAAGGATCTTCCTTTGGCTGCATTCACTTATGTAGATGTGTATTCTGTCAAATACTCTCTTTTCAAGGAACCCGAAAAGTATGGATTTGAGCTCCCACTTGTTGCTTGTTGTGGCTCCGGTGGTAAGTACAACTATAACGCCACTGCTGGATGTGGAGCAATAATTACAGTCAATGGAAGCCAAATCCTTGTTGGTTCATGCAAAAACCCCTCAATTAGAGTGAACTGGGATGGTATTCATTACACCGAGGCAGCTGCGAAATTTGTTTTTGATAAAATTTCAACTGGAGCGTATTCAGATCCACCTCTTCCCTTGAAACAAGCATGTCACAGGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005488 GO:0006950 GO:0006952 GO:0008150 GO:0016787 GO:0016788 GO:0019863 GO:0019865 GO:0044877 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

385

Amino Acids

42.17

Weight (kDa)

6.74

Isoelectric Point (pI)

38.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 39 - 366 4e-46 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 383
AciI CCGC 1 cut(s) 159
AclWI GGATC 5 cut(s) 70, 664, 677, 831, 1112
AcoI YGGCCR 2 cut(s) 213, 535
AcsI RAATTY 5 cut(s) 136, 310, 328, 1079, 1095
AcuI CTGAAG 1 cut(s) 815
AfaI GTAC 3 cut(s) 8, 503, 947
AfiI CCNNNNNNNGG 4 cut(s) 85, 211, 383, 679
AgsI TTSAA 7 cut(s) 94, 121, 413, 812, 883, 1101, 1135
AluBI AGCT 7 cut(s) 344, 515, 728, 773, 793, 910, 1073
AluI AGCT 7 cut(s) 344, 515, 728, 773, 793, 910, 1073
Alw21I GWGCWC 1 cut(s) 912
Alw26I GTCTC 3 cut(s) 123, 188, 701
AlwI GGATC 5 cut(s) 70, 664, 677, 831, 1112
AlwNI CAGNNNCTG 2 cut(s) 146, 427
Ama87I CYCGRG 1 cut(s) 302
AoxI GGCC 4 cut(s) 213, 535, 683, 801
ApeKI GCWGC 4 cut(s) 341, 836, 1070, 1073
ApoI RAATTY 5 cut(s) 136, 310, 328, 1079, 1095
ArsI GACNNNNNNTTYG 2 cut(s) 707, 739
AspLEI GCGC 1 cut(s) 287
AspS9I GGNCC 1 cut(s) 683
AsuHPI GGTGA 1 cut(s) 263
AvaI CYCGRG 1 cut(s) 302
BalI TGGCCA 1 cut(s) 537
BamHI GGATCC 1 cut(s) 669
BanII GRGCYC 1 cut(s) 912
BauI CACGAG 1 cut(s) 788
BbsI GAAGAC 1 cut(s) 241
Bbv12I GWGCWC 1 cut(s) 912
BbvI GCAGC 4 cut(s) 353, 823, 1060, 1082
BccI CCATC 3 cut(s) 122, 224, 1043
BcgI CGANNNNNNTGC 4 cut(s) 236, 270, 1055, 1089
BcoDI GTCTC 3 cut(s) 123, 188, 701
BfaI CTAG 1 cut(s) 155
BfmI CTRYAG 1 cut(s) 339
BglII AGATCT 1 cut(s) 364
BisI GCNGC 5 cut(s) 159, 342, 837, 1071, 1074
BlsI GCNGC 5 cut(s) 160, 343, 838, 1072, 1075
BmcAI AGTACT 1 cut(s) 503
BmeT110I CYCGRG 1 cut(s) 302
BmgT120I GGNCC 1 cut(s) 683
BmiI GGNNCC 4 cut(s) 671, 684, 888, 934
BmrI ACTGGG 1 cut(s) 1054
BmsI GCATC 3 cut(s) 170, 469, 602
BmuI ACTGGG 1 cut(s) 1054
BpiI GAAGAC 1 cut(s) 241
BplI GAGNNNNNCTC 2 cut(s) 491, 523
BpmI CTGGAG 1 cut(s) 1125
Bsa29I ATCGAT 1 cut(s) 246
BsaI GGTCTC 1 cut(s) 188
BsaJI CCNNGG 3 cut(s) 84, 492, 1065
BsaWI WCCGGW 1 cut(s) 935
Bsc4I CCNNNNNNNGG 4 cut(s) 85, 211, 383, 679
Bse118I RCCGGY 2 cut(s) 211, 380
Bse1I ACTGG 5 cut(s) 63, 151, 619, 1049, 1108
BseCI ATCGAT 1 cut(s) 246
BseDI CCNNGG 3 cut(s) 84, 492, 1065
BseGI GGATG 3 cut(s) 161, 974, 1054
BseLI CCNNNNNNNGG 4 cut(s) 85, 211, 383, 679
BseMII CTCAG 2 cut(s) 720, 829
BseNI ACTGG 5 cut(s) 63, 151, 619, 1049, 1108
BseRI GAGGAG 1 cut(s) 512
BseX3I CGGCCG 1 cut(s) 213
BseXI GCAGC 4 cut(s) 353, 823, 1060, 1082
Bsh1285I CGRYCG 1 cut(s) 216
BshFI GGCC 4 cut(s) 215, 537, 685, 803
BshVI ATCGAT 1 cut(s) 246
BsiEI CGRYCG 1 cut(s) 216
BsiHKAI GWGCWC 1 cut(s) 912
BsiHKCI CYCGRG 1 cut(s) 302
BsiSI CCGG 4 cut(s) 212, 216, 381, 936
BslFI GGGAC 2 cut(s) 319, 378
BslI CCNNNNNNNGG 4 cut(s) 85, 211, 383, 679
BsmAI GTCTC 3 cut(s) 123, 188, 701
BsmFI GGGAC 2 cut(s) 319, 378
BsmI GAATGC 1 cut(s) 839
BsnI GGCC 4 cut(s) 215, 537, 685, 803
Bso31I GGTCTC 1 cut(s) 188
BsoBI CYCGRG 1 cut(s) 302
Bsp1286I GDGCHC 1 cut(s) 912
Bsp143I GATC 8 cut(s) 75, 364, 443, 544, 659, 669, 823, 1117
BspACI CCGC 1 cut(s) 159
BspANI GGCC 4 cut(s) 215, 537, 685, 803
BspCNI CTCAG 2 cut(s) 721, 828
BspDI ATCGAT 1 cut(s) 246
BspLI GGNNCC 4 cut(s) 671, 684, 888, 934
BspMAI CTGCAG 1 cut(s) 343
BspPI GGATC 5 cut(s) 70, 664, 677, 831, 1112
BspTNI GGTCTC 1 cut(s) 188
BsrFI RCCGGY 2 cut(s) 211, 380
BsrI ACTGG 5 cut(s) 63, 151, 619, 1049, 1108
BssAI RCCGGY 2 cut(s) 211, 380
BssECI CCNNGG 3 cut(s) 84, 492, 1065
BssMI GATC 8 cut(s) 75, 364, 443, 544, 659, 669, 823, 1117
BssSI CACGAG 1 cut(s) 788
BssT1I CCWWGG 2 cut(s) 84, 492
Bst2BI CACGAG 1 cut(s) 788
Bst4CI ACNGT 2 cut(s) 101, 988
Bst6I CTCTTC 1 cut(s) 1131
BstAPI GCANNNNNTGC 2 cut(s) 332, 427
BstC8I GCNNGC 2 cut(s) 213, 801
BstDEI CTNAG 3 cut(s) 281, 729, 815
BstEII GGTNACC 2 cut(s) 269, 566
BstF5I GGATG 3 cut(s) 161, 974, 1054
BstHHI GCGC 1 cut(s) 287
BstKTI GATC 8 cut(s) 78, 367, 446, 547, 662, 672, 826, 1120
BstMAI GTCTC 3 cut(s) 123, 188, 701
BstMBI GATC 8 cut(s) 75, 364, 443, 544, 659, 669, 823, 1117
BstMCI CGRYCG 1 cut(s) 216
BstMWI GCNNNNNNNGC 4 cut(s) 52, 332, 341, 427
BstNSI RCATGY 1 cut(s) 1146
BstPI GGTNACC 2 cut(s) 269, 566
BstSFI CTRYAG 1 cut(s) 339
BstV1I GCAGC 4 cut(s) 353, 823, 1060, 1082
BstV2I GAAGAC 1 cut(s) 241
BstX2I RGATCY 4 cut(s) 364, 669, 823, 1117
BstYI RGATCY 4 cut(s) 364, 669, 823, 1117
BstZI CGGCCG 1 cut(s) 213
Bsu15I ATCGAT 1 cut(s) 246
BsuRI GGCC 4 cut(s) 215, 537, 685, 803
BsuTUI ATCGAT 1 cut(s) 246
BtsCI GGATG 3 cut(s) 161, 974, 1054
BtsI GCAGTG 1 cut(s) 960
BtsIMutI CAGTG 2 cut(s) 612, 960
Cac8I GCNNGC 2 cut(s) 213, 801
CaiI CAGNNNCTG 2 cut(s) 146, 427
CfoI GCGC 1 cut(s) 287
Cfr10I RCCGGY 2 cut(s) 211, 380
Cfr13I GGNCC 1 cut(s) 683
ClaI ATCGAT 1 cut(s) 246
Csp6I GTAC 3 cut(s) 7, 502, 946
CviAII CATG 2 cut(s) 1017, 1143
CviQI GTAC 3 cut(s) 7, 502, 946
DdeI CTNAG 3 cut(s) 281, 729, 815
DpnI GATC 8 cut(s) 77, 366, 445, 546, 661, 671, 825, 1119
DpnII GATC 8 cut(s) 75, 364, 443, 544, 659, 669, 823, 1117
EaeI YGGCCR 2 cut(s) 213, 535
EagI CGGCCG 1 cut(s) 213
Eam1104I CTCTTC 1 cut(s) 1131
EarI CTCTTC 1 cut(s) 1131
Ecl136II GAGCTC 1 cut(s) 910
EclXI CGGCCG 1 cut(s) 213
Eco130I CCWWGG 2 cut(s) 84, 492
Eco24I GRGCYC 1 cut(s) 912
Eco31I GGTCTC 1 cut(s) 188
Eco52I CGGCCG 1 cut(s) 213
Eco53kI GAGCTC 1 cut(s) 910
Eco57I CTGAAG 1 cut(s) 815
Eco88I CYCGRG 1 cut(s) 302
Eco91I GGTNACC 2 cut(s) 269, 566
EcoICRI GAGCTC 1 cut(s) 910
EcoO65I GGTNACC 2 cut(s) 269, 566
EcoT14I CCWWGG 2 cut(s) 84, 492
EcoT22I ATGCAT 1 cut(s) 595
EcoT38I GRGCYC 1 cut(s) 912
ErhI CCWWGG 2 cut(s) 84, 492
FaeI CATG 2 cut(s) 1020, 1146
FaqI GGGAC 2 cut(s) 319, 378
FatI CATG 2 cut(s) 1016, 1142
FauNDI CATATG 1 cut(s) 207
Fnu4HI GCNGC 5 cut(s) 159, 342, 837, 1071, 1074
FokI GGATG 3 cut(s) 148, 981, 1061
FriOI GRGCYC 1 cut(s) 912
Fsp4HI GCNGC 5 cut(s) 159, 342, 837, 1071, 1074
FspBI CTAG 1 cut(s) 155
GlaI GCGC 1 cut(s) 286
GluI GCNGC 5 cut(s) 159, 342, 837, 1071, 1074
GsuI CTGGAG 1 cut(s) 1125
HaeIII GGCC 4 cut(s) 215, 537, 685, 803
HapII CCGG 4 cut(s) 212, 216, 381, 936
HhaI GCGC 1 cut(s) 287
Hin1II CATG 2 cut(s) 1020, 1146
Hin6I GCGC 1 cut(s) 285
HinP1I GCGC 1 cut(s) 285
HindIII AAGCTT 1 cut(s) 513
HinfI GANTC 6 cut(s) 131, 222, 237, 262, 296, 357
HpaII CCGG 4 cut(s) 212, 216, 381, 936
HphI GGTGA 1 cut(s) 263
Hpy166II GTNNAC 2 cut(s) 152, 1042
Hpy188I TCNGA 5 cut(s) 128, 142, 229, 356, 1117
Hpy188III TCNNGA 6 cut(s) 293, 304, 441, 602, 634, 817
Hpy8I GTNNAC 2 cut(s) 152, 1042
HpyAV CCTTC 3 cut(s) 176, 406, 814
HpyCH4III ACNGT 2 cut(s) 101, 988
HpyF10VI GCNNNNNNNGC 4 cut(s) 52, 332, 341, 427
HpyF3I CTNAG 3 cut(s) 281, 729, 815
Hsp92II CATG 2 cut(s) 1020, 1146
HspAI GCGC 1 cut(s) 285
KroI GCCGGC 1 cut(s) 211
KroNI GCCGGC 1 cut(s) 213
Kzo9I GATC 8 cut(s) 75, 364, 443, 544, 659, 669, 823, 1117
LmnI GCTCC 6 cut(s) 41, 653, 915, 938, 974, 1106
Lsp1109I GCAGC 4 cut(s) 353, 823, 1060, 1082
LweI GCATC 3 cut(s) 170, 469, 602
MaeI CTAG 1 cut(s) 155
MaeIII GTNAC 3 cut(s) 269, 566, 1145
MalI GATC 8 cut(s) 77, 366, 445, 546, 661, 671, 825, 1119
MboI GATC 8 cut(s) 75, 364, 443, 544, 659, 669, 823, 1117
MboII GAAGA 5 cut(s) 109, 231, 246, 818, 1118
MflI RGATCY 4 cut(s) 364, 669, 823, 1117
MhlI GDGCHC 1 cut(s) 912
MlsI TGGCCA 1 cut(s) 537
MluCI AATT 7 cut(s) 136, 310, 328, 981, 1032, 1079, 1095
MluNI TGGCCA 1 cut(s) 537
MlyI GAGTC 4 cut(s) 125, 231, 271, 351
MnlI CCTC 6 cut(s) 458, 490, 547, 1039, 1060, 1134
Mox20I TGGCCA 1 cut(s) 537
Mph1103I ATGCAT 1 cut(s) 595
MroNI GCCGGC 1 cut(s) 211
MscI TGGCCA 1 cut(s) 537
MseI TTAA 2 cut(s) 485, 783
Msp20I TGGCCA 1 cut(s) 537
MspA1I CMGCKG 2 cut(s) 728, 1073
MspI CCGG 4 cut(s) 212, 216, 381, 936
Mva1269I GAATGC 1 cut(s) 839
MwoI GCNNNNNNNGC 4 cut(s) 52, 332, 341, 427
NaeI GCCGGC 1 cut(s) 213
NdeI CATATG 1 cut(s) 207
NdeII GATC 8 cut(s) 75, 364, 443, 544, 659, 669, 823, 1117
NgoMIV GCCGGC 1 cut(s) 211
NlaIII CATG 2 cut(s) 1020, 1146
NlaIV GGNNCC 4 cut(s) 671, 684, 888, 934
NmuCI GTSAC 2 cut(s) 269, 1145
NsiI ATGCAT 1 cut(s) 595
NspI RCATGY 1 cut(s) 1146
PctI GAATGC 1 cut(s) 839
PdiI GCCGGC 1 cut(s) 213
PfeI GAWTC 2 cut(s) 222, 296
PflMI CCANNNNNTGG 1 cut(s) 383
PkrI GCNGC 5 cut(s) 160, 343, 838, 1072, 1075
PleI GAGTC 4 cut(s) 125, 231, 270, 351
PpsI GAGTC 4 cut(s) 125, 231, 270, 351
Psp124BI GAGCTC 1 cut(s) 912
PspEI GGTNACC 2 cut(s) 269, 566
PspN4I GGNNCC 4 cut(s) 671, 684, 888, 934
PspPI GGNCC 1 cut(s) 683
PstI CTGCAG 1 cut(s) 343
PstNI CAGNNNCTG 2 cut(s) 146, 427
PsuI RGATCY 4 cut(s) 364, 669, 823, 1117
PvuII CAGCTG 2 cut(s) 728, 1073
RsaI GTAC 3 cut(s) 8, 503, 947
RsaNI GTAC 3 cut(s) 7, 502, 946
SacI GAGCTC 1 cut(s) 912
SaqAI TTAA 2 cut(s) 485, 783
SatI GCNGC 5 cut(s) 159, 342, 837, 1071, 1074
Sau3AI GATC 8 cut(s) 75, 364, 443, 544, 659, 669, 823, 1117
Sau96I GGNCC 1 cut(s) 683
ScaI AGTACT 1 cut(s) 503
SchI GAGTC 4 cut(s) 125, 231, 271, 351
SduI GDGCHC 1 cut(s) 912
SfaNI GCATC 3 cut(s) 170, 469, 602
SfcI CTRYAG 1 cut(s) 339
Sse9I AATT 7 cut(s) 136, 310, 328, 981, 1032, 1079, 1095
SsiI CCGC 1 cut(s) 159
SspI AATATT 1 cut(s) 474
SspMI CTAG 1 cut(s) 155
SstI GAGCTC 1 cut(s) 912
StyI CCWWGG 2 cut(s) 84, 492
TaaI ACNGT 2 cut(s) 101, 988
TaqI TCGA 3 cut(s) 246, 463, 528
TasI AATT 7 cut(s) 136, 310, 328, 981, 1032, 1079, 1095
TatI WGTACW 2 cut(s) 501, 945
TauI GCSGC 1 cut(s) 161
TfiI GAWTC 2 cut(s) 222, 296
Tru1I TTAA 2 cut(s) 485, 783
Tru9I TTAA 2 cut(s) 485, 783
TscAI CASTG 2 cut(s) 619, 967
TseFI GTSAC 2 cut(s) 269, 1145
TseI GCWGC 4 cut(s) 341, 836, 1070, 1073
Tsp45I GTSAC 2 cut(s) 269, 1145
TspDTI ATGAA 3 cut(s) 780, 1005, 1046
TspRI CASTG 2 cut(s) 619, 967
Van91I CCANNNNNTGG 1 cut(s) 383
XapI RAATTY 5 cut(s) 136, 310, 328, 1079, 1095
XceI RCATGY 1 cut(s) 1146
XcmI CCANNNNNNNNNTGG 2 cut(s) 545, 1007
XspI CTAG 1 cut(s) 155
ZrmI AGTACT 1 cut(s) 503
Zsp2I ATGCAT 1 cut(s) 595
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.