RchiOBHm_Chr6g0295731

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000978
Physical Location & Seq
Reverse (-)
57657865 .. 57660507
2643 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26541

Sequence Viewer

Length: 1284 bp
ATGCCTCCTAGAAATACAGTAGAAGTAGATTGCACCTTTCCTTACACTATAAAACAGGCACAGCCGCACAGCTTAGTTCTCATCTGCATCTTTTGGTATTTCTCCACTATGGAGTACACTCCTAGAGTTCTTATTTCTCTGTTCGGCTGCTATATGCTCCTATTGCATATTACCAGTATTACTGTAAGCCCTGCCTTGGCTTTGGAAGACTGCGAGTTTCCAGCCATCTTCAACTTCGGAGACTCAAATTCGGATACTGGTGGATTCGCAGCATCCATTTATCCCCCACCTACACCTTATGGAGAGACCTATTTTCATATGCCGGTTGGAAGAATGTCAGATGGAAGACTCATTGTCGATTTCCTCGCAAAGAGTCTTGGTCATCCTTATCTAAGCGCATATCTAGATTCCCTCGGGACCAATTTCTCGCATGGTGCAAATTTTGCCACTTCAGCTTCCACTATCAGACTCCCAGATCTCATTTTACCAGCCGGCGGATTTAGTCCCTTCTACCTTGATATTCAATACGTACAGTTCCGGGAACTAAAGTCCAGATCACAACTCATAAGGCAACGAGGGGGAATATTTGCTAGTTTAATGCCCAAAGAGGAGTACTTCTCCAAAGCTTTATACACATTCGACATTGGTCAGAATGATCTTGGCGAGGGATTTTTCGGTAACAAGACTATACAGGAAGTCAATGCATCTGTTCCTGATATAATCACTGGTTTCTCAACGAATATCAAGAAAATATATGATTTGGGGGCAAGATCATTTTGGATCCACAATACGGGGCCGATTGGCTGTGTCCCTTACATTTTGGTGAAATTCCCATCGGCTCAGAAAGATGAGGTTGGCTGTGCAAAGGCTTACAATGGAGTAGCTCAACATTTTAACCACGAGCTGAAGCAGGCCATAGTTCAACTCAGGAAGGATCTTCCTTTGGCTGCATTCACTTATGTAGATGTGTATTCCGTCAAATATTCTATTTTCAAGGAACCCAAAAAATATGGATTTGAGCTCCCACTTGTTGCTTGTTGTGGCTATGGTGGTAAGTACAACTATAACAGTAGTACAGGATGTGGAGCAACAATCACAGTCAATGGAAGTCAAATCTTTGTTGGCTCATGCAAGAACCCATCAGCTAGAGTGAACTGGGATGGGATTCATTATACTGAGGCAGCAGCGAAATTTGTTTTTGAGAAAATCTCAACTGGAGCATATTCAGATCCACCTCTGCCATTGAAACAAGCATGCCACAGGAGTGGAAATAAGAAAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005488 GO:0006950 GO:0006952 GO:0008150 GO:0016787 GO:0016788 GO:0019863 GO:0019865 GO:0044877 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

427

Amino Acids

47.3

Weight (kDa)

8.35

Isoelectric Point (pI)

37.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 76 - 403 2.4e-45 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 65, 495
AclWI GGATC 4 cut(s) 775, 788, 942, 1223
AcsI RAATTY 4 cut(s) 247, 439, 827, 1190
AcuI CTGAAG 2 cut(s) 435, 926
AfaI GTAC 5 cut(s) 116, 531, 614, 1058, 1075
AfiI CCNNNNNNNGG 3 cut(s) 196, 494, 790
AgsI TTSAA 5 cut(s) 232, 524, 923, 994, 1246
AhdI GACNNNNNGTC 1 cut(s) 353
AleI CACNNNNGTG 1 cut(s) 1263
AluBI AGCT 7 cut(s) 72, 455, 626, 884, 904, 1021, 1145
AluI AGCT 7 cut(s) 72, 455, 626, 884, 904, 1021, 1145
Alw21I GWGCWC 1 cut(s) 1023
Alw26I GTCTC 2 cut(s) 234, 299
AlwI GGATC 4 cut(s) 775, 788, 942, 1223
Ama87I CYCGRG 1 cut(s) 413
AoxI GGCC 2 cut(s) 794, 912
ApeKI GCWGC 5 cut(s) 147, 269, 947, 1181, 1184
ApoI RAATTY 4 cut(s) 247, 439, 827, 1190
AspLEI GCGC 1 cut(s) 398
AspS9I GGNCC 2 cut(s) 417, 794
AsuC2I CCSGG 1 cut(s) 539
AsuHPI GGTGA 1 cut(s) 835
AvaI CYCGRG 1 cut(s) 413
AvaII GGWCC 1 cut(s) 417
BamHI GGATCC 1 cut(s) 780
BanII GRGCYC 1 cut(s) 1023
BauI CACGAG 1 cut(s) 899
BbsI GAAGAC 2 cut(s) 213, 352
Bbv12I GWGCWC 1 cut(s) 1023
BbvI GCAGC 5 cut(s) 134, 281, 934, 1193, 1196
BccI CCATC 5 cut(s) 233, 335, 841, 1147, 1154
BciVI GTATCC 1 cut(s) 247
BcnI CCSGG 1 cut(s) 539
BcoDI GTCTC 2 cut(s) 234, 299
BfaI CTAG 5 cut(s) 9, 123, 404, 591, 1146
BfuI GTATCC 1 cut(s) 247
BglII AGATCT 1 cut(s) 475
BisI GCNGC 6 cut(s) 65, 148, 270, 948, 1182, 1185
BlsI GCNGC 6 cut(s) 66, 149, 271, 949, 1183, 1186
BmcAI AGTACT 1 cut(s) 614
Bme1390I CCNGG 1 cut(s) 539
Bme18I GGWCC 1 cut(s) 417
BmeRI GACNNNNNGTC 1 cut(s) 353
BmeT110I CYCGRG 1 cut(s) 413
BmgT120I GGNCC 2 cut(s) 417, 794
BmiI GGNNCC 4 cut(s) 418, 782, 795, 999
BmrFI CCNGG 1 cut(s) 539
BmrI ACTGGG 1 cut(s) 1165
BmsI GCATC 3 cut(s) 96, 281, 713
BmuI ACTGGG 1 cut(s) 1165
BoxI GACNNNNGTC 1 cut(s) 645
BpiI GAAGAC 2 cut(s) 213, 352
BplI GAGNNNNNCTC 4 cut(s) 602, 634, 1193, 1225
BpmI CTGGAG 1 cut(s) 1236
BpuMI CCSGG 1 cut(s) 539
BsaAI YACGTR 1 cut(s) 529
BsaI GGTCTC 1 cut(s) 299
BsaJI CCNNGG 2 cut(s) 195, 412
Bsc4I CCNNNNNNNGG 3 cut(s) 196, 494, 790
Bse118I RCCGGY 2 cut(s) 322, 491
Bse1I ACTGG 5 cut(s) 174, 262, 730, 1160, 1219
BseDI CCNNGG 2 cut(s) 195, 412
BseGI GGATG 4 cut(s) 272, 382, 1085, 1165
BseLI CCNNNNNNNGG 3 cut(s) 196, 494, 790
BseMII CTCAG 3 cut(s) 854, 940, 1167
BseNI ACTGG 5 cut(s) 174, 262, 730, 1160, 1219
BseRI GAGGAG 1 cut(s) 623
BseXI GCAGC 5 cut(s) 134, 281, 934, 1193, 1196
BshFI GGCC 2 cut(s) 796, 914
BsiHKAI GWGCWC 1 cut(s) 1023
BsiHKCI CYCGRG 1 cut(s) 413
BsiSI CCGG 3 cut(s) 323, 492, 538
BslFI GGGAC 3 cut(s) 430, 489, 794
BslI CCNNNNNNNGG 3 cut(s) 196, 494, 790
BsmAI GTCTC 2 cut(s) 234, 299
BsmFI GGGAC 3 cut(s) 430, 489, 794
BsmI GAATGC 1 cut(s) 950
BsnI GGCC 2 cut(s) 796, 914
Bso31I GGTCTC 1 cut(s) 299
BsoBI CYCGRG 1 cut(s) 413
Bsp1286I GDGCHC 1 cut(s) 1023
Bsp143I GATC 7 cut(s) 475, 554, 655, 770, 780, 934, 1228
BspACI CCGC 2 cut(s) 65, 495
BspANI GGCC 2 cut(s) 796, 914
BspCNI CTCAG 3 cut(s) 853, 939, 1168
BspLI GGNNCC 4 cut(s) 418, 782, 795, 999
BspPI GGATC 4 cut(s) 775, 788, 942, 1223
BspTNI GGTCTC 1 cut(s) 299
BsrFI RCCGGY 2 cut(s) 322, 491
BsrI ACTGG 5 cut(s) 174, 262, 730, 1160, 1219
BssAI RCCGGY 2 cut(s) 322, 491
BssECI CCNNGG 2 cut(s) 195, 412
BssMI GATC 7 cut(s) 475, 554, 655, 770, 780, 934, 1228
BssSI CACGAG 1 cut(s) 899
BssT1I CCWWGG 1 cut(s) 195
Bst2BI CACGAG 1 cut(s) 899
Bst4CI ACNGT 5 cut(s) 19, 184, 534, 1070, 1099
BstAPI GCANNNNNTGC 1 cut(s) 443
BstBAI YACGTR 1 cut(s) 529
BstC8I GCNNGC 3 cut(s) 493, 912, 1255
BstDEI CTNAG 5 cut(s) 73, 392, 840, 926, 1176
BstF5I GGATG 4 cut(s) 272, 382, 1085, 1165
BstHHI GCGC 1 cut(s) 398
BstKTI GATC 7 cut(s) 478, 557, 658, 773, 783, 937, 1231
BstMAI GTCTC 2 cut(s) 234, 299
BstMBI GATC 7 cut(s) 475, 554, 655, 770, 780, 934, 1228
BstMWI GCNNNNNNNGC 3 cut(s) 163, 443, 452
BstNSI RCATGY 1 cut(s) 1257
BstPAI GACNNNNGTC 1 cut(s) 645
BstSCI CCNGG 1 cut(s) 537
BstSNI TACGTA 1 cut(s) 529
BstV1I GCAGC 5 cut(s) 134, 281, 934, 1193, 1196
BstV2I GAAGAC 2 cut(s) 213, 352
BstX2I RGATCY 4 cut(s) 475, 780, 934, 1228
BstXI CCANNNNNNTGG 1 cut(s) 1265
BstYI RGATCY 4 cut(s) 475, 780, 934, 1228
BsuI GTATCC 1 cut(s) 247
BsuRI GGCC 2 cut(s) 796, 914
BtsCI GGATG 4 cut(s) 272, 382, 1085, 1165
BtsIMutI CAGTG 1 cut(s) 723
Cac8I GCNNGC 3 cut(s) 493, 912, 1255
CfoI GCGC 1 cut(s) 398
Cfr10I RCCGGY 2 cut(s) 322, 491
Cfr13I GGNCC 2 cut(s) 417, 794
Csp6I GTAC 5 cut(s) 115, 530, 613, 1057, 1074
CviAII CATG 3 cut(s) 431, 1128, 1254
CviQI GTAC 5 cut(s) 115, 530, 613, 1057, 1074
DdeI CTNAG 5 cut(s) 73, 392, 840, 926, 1176
DpnI GATC 7 cut(s) 477, 556, 657, 772, 782, 936, 1230
DpnII GATC 7 cut(s) 475, 554, 655, 770, 780, 934, 1228
DriI GACNNNNNGTC 1 cut(s) 353
Eam1105I GACNNNNNGTC 1 cut(s) 353
EciI GGCGGA 1 cut(s) 510
Ecl136II GAGCTC 1 cut(s) 1021
Eco105I TACGTA 1 cut(s) 529
Eco130I CCWWGG 1 cut(s) 195
Eco24I GRGCYC 1 cut(s) 1023
Eco31I GGTCTC 1 cut(s) 299
Eco47I GGWCC 1 cut(s) 417
Eco53kI GAGCTC 1 cut(s) 1021
Eco57I CTGAAG 2 cut(s) 435, 926
Eco88I CYCGRG 1 cut(s) 413
EcoICRI GAGCTC 1 cut(s) 1021
EcoT14I CCWWGG 1 cut(s) 195
EcoT22I ATGCAT 1 cut(s) 706
EcoT38I GRGCYC 1 cut(s) 1023
ErhI CCWWGG 1 cut(s) 195
FaeI CATG 3 cut(s) 434, 1131, 1257
FaqI GGGAC 3 cut(s) 430, 489, 794
FatI CATG 3 cut(s) 430, 1127, 1253
FauNDI CATATG 1 cut(s) 318
Fnu4HI GCNGC 6 cut(s) 65, 148, 270, 948, 1182, 1185
FokI GGATG 4 cut(s) 259, 369, 1092, 1172
FriOI GRGCYC 1 cut(s) 1023
Fsp4HI GCNGC 6 cut(s) 65, 148, 270, 948, 1182, 1185
FspBI CTAG 5 cut(s) 9, 123, 404, 591, 1146
GlaI GCGC 1 cut(s) 397
GluI GCNGC 6 cut(s) 65, 148, 270, 948, 1182, 1185
GsuI CTGGAG 1 cut(s) 1236
HaeIII GGCC 2 cut(s) 796, 914
HapII CCGG 3 cut(s) 323, 492, 538
HhaI GCGC 1 cut(s) 398
Hin1II CATG 3 cut(s) 434, 1131, 1257
Hin6I GCGC 1 cut(s) 396
HinP1I GCGC 1 cut(s) 396
HindIII AAGCTT 1 cut(s) 624
HinfI GANTC 7 cut(s) 242, 264, 348, 373, 407, 468, 1165
HpaII CCGG 3 cut(s) 323, 492, 538
HphI GGTGA 1 cut(s) 835
Hpy166II GTNNAC 2 cut(s) 117, 1153
Hpy188I TCNGA 7 cut(s) 239, 253, 340, 467, 651, 843, 1228
Hpy188III TCNNGA 6 cut(s) 404, 415, 552, 713, 745, 928
Hpy8I GTNNAC 2 cut(s) 117, 1153
HpyAV CCTTC 2 cut(s) 517, 925
HpyCH4III ACNGT 5 cut(s) 19, 184, 534, 1070, 1099
HpyCH4IV ACGT 1 cut(s) 528
HpyCH4V TGCA 8 cut(s) 33, 87, 166, 437, 704, 863, 950, 1131
HpyF10VI GCNNNNNNNGC 3 cut(s) 163, 443, 452
HpyF3I CTNAG 5 cut(s) 73, 392, 840, 926, 1176
HpySE526I ACGT 1 cut(s) 528
Hsp92II CATG 3 cut(s) 434, 1131, 1257
HspAI GCGC 1 cut(s) 396
KroI GCCGGC 1 cut(s) 491
KroNI GCCGGC 1 cut(s) 493
Kzo9I GATC 7 cut(s) 475, 554, 655, 770, 780, 934, 1228
LmnI GCTCC 4 cut(s) 162, 1026, 1085, 1217
Lsp1109I GCAGC 5 cut(s) 134, 281, 934, 1193, 1196
LweI GCATC 3 cut(s) 96, 281, 713
MaeI CTAG 5 cut(s) 9, 123, 404, 591, 1146
MaeII ACGT 1 cut(s) 528
MaeIII GTNAC 1 cut(s) 677
MalI GATC 7 cut(s) 477, 556, 657, 772, 782, 936, 1230
MboI GATC 7 cut(s) 475, 554, 655, 770, 780, 934, 1228
MboII GAAGA 5 cut(s) 218, 220, 342, 357, 929
MflI RGATCY 4 cut(s) 475, 780, 934, 1228
MhlI GDGCHC 1 cut(s) 1023
MluCI AATT 5 cut(s) 247, 421, 439, 827, 1190
MlyI GAGTC 4 cut(s) 236, 342, 382, 462
MmeI TCCRAC 1 cut(s) 307
MnlI CCTC 9 cut(s) 15, 374, 422, 569, 601, 658, 844, 1171, 1245
Mph1103I ATGCAT 1 cut(s) 706
MroNI GCCGGC 1 cut(s) 491
MseI TTAA 2 cut(s) 596, 894
MslI CAYNNNNRTG 2 cut(s) 821, 1263
MspI CCGG 3 cut(s) 323, 492, 538
MspR9I CCNGG 1 cut(s) 539
Mva1269I GAATGC 1 cut(s) 950
MwoI GCNNNNNNNGC 3 cut(s) 163, 443, 452
NaeI GCCGGC 1 cut(s) 493
NciI CCSGG 1 cut(s) 539
NdeI CATATG 1 cut(s) 318
NdeII GATC 7 cut(s) 475, 554, 655, 770, 780, 934, 1228
NgoMIV GCCGGC 1 cut(s) 491
NlaIII CATG 3 cut(s) 434, 1131, 1257
NlaIV GGNNCC 4 cut(s) 418, 782, 795, 999
NsiI ATGCAT 1 cut(s) 706
NspI RCATGY 1 cut(s) 1257
OliI CACNNNNGTG 1 cut(s) 1263
PaeI GCATGC 1 cut(s) 1257
PctI GAATGC 1 cut(s) 950
PdiI GCCGGC 1 cut(s) 493
PfeI GAWTC 3 cut(s) 264, 407, 1165
PfoI TCCNGGA 1 cut(s) 537
PkrI GCNGC 6 cut(s) 66, 149, 271, 949, 1183, 1186
PleI GAGTC 4 cut(s) 236, 342, 381, 462
PpsI GAGTC 4 cut(s) 236, 342, 381, 462
Ppu21I YACGTR 1 cut(s) 529
PshAI GACNNNNGTC 1 cut(s) 645
Psp124BI GAGCTC 1 cut(s) 1023
PspN4I GGNNCC 4 cut(s) 418, 782, 795, 999
PspPI GGNCC 2 cut(s) 417, 794
PsuI RGATCY 4 cut(s) 475, 780, 934, 1228
RsaI GTAC 5 cut(s) 116, 531, 614, 1058, 1075
RsaNI GTAC 5 cut(s) 115, 530, 613, 1057, 1074
RseI CAYNNNNRTG 2 cut(s) 821, 1263
SacI GAGCTC 1 cut(s) 1023
SaqAI TTAA 2 cut(s) 596, 894
SatI GCNGC 6 cut(s) 65, 148, 270, 948, 1182, 1185
Sau3AI GATC 7 cut(s) 475, 554, 655, 770, 780, 934, 1228
Sau96I GGNCC 2 cut(s) 417, 794
ScaI AGTACT 1 cut(s) 614
SchI GAGTC 4 cut(s) 236, 342, 382, 462
ScrFI CCNGG 1 cut(s) 539
SduI GDGCHC 1 cut(s) 1023
SfaNI GCATC 3 cut(s) 96, 281, 713
SinI GGWCC 1 cut(s) 417
SmiMI CAYNNNNRTG 2 cut(s) 821, 1263
SnaBI TACGTA 1 cut(s) 529
SphI GCATGC 1 cut(s) 1257
Sse9I AATT 5 cut(s) 247, 421, 439, 827, 1190
SsiI CCGC 2 cut(s) 65, 495
SspI AATATT 2 cut(s) 585, 983
SspMI CTAG 5 cut(s) 9, 123, 404, 591, 1146
SstI GAGCTC 1 cut(s) 1023
StyD4I CCNGG 1 cut(s) 537
StyI CCWWGG 1 cut(s) 195
TaaI ACNGT 5 cut(s) 19, 184, 534, 1070, 1099
TaiI ACGT 1 cut(s) 531
TaqI TCGA 2 cut(s) 357, 639
TasI AATT 5 cut(s) 247, 421, 439, 827, 1190
TatI WGTACW 4 cut(s) 114, 612, 1056, 1073
TauI GCSGC 1 cut(s) 67
TfiI GAWTC 3 cut(s) 264, 407, 1165
Tru1I TTAA 2 cut(s) 596, 894
Tru9I TTAA 2 cut(s) 596, 894
TscAI CASTG 1 cut(s) 730
TseI GCWGC 5 cut(s) 147, 269, 947, 1181, 1184
TspDTI ATGAA 2 cut(s) 305, 1157
TspGWI ACGGA 1 cut(s) 964
TspRI CASTG 1 cut(s) 730
VpaK11BI GGWCC 1 cut(s) 417
XapI RAATTY 4 cut(s) 247, 439, 827, 1190
XbaI TCTAGA 1 cut(s) 403
XceI RCATGY 1 cut(s) 1257
XspI CTAG 5 cut(s) 9, 123, 404, 591, 1146
ZrmI AGTACT 1 cut(s) 614
Zsp2I ATGCAT 1 cut(s) 706
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.