Rorug06G0254000

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000978
Physical Location & Seq
Reverse (-)
40042778 .. 40043323
546 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0254000.1

Sequence Viewer

Length: 546 bp
ATGCAGATGCTTGGCATTAGAGCGAGACTGGAGGAGCTCTTTGATGTTCGGTGTTCAACTGCAATGACAGAAGAGAAGAAACAGTTGATGGAGGATTTGCAGCGACTTCTTTCACAAGAAGAGAGCTTTTGGAAACAACGCTCTAAAGTACAATGGTTAAAGGAAGAGGATCGGAACACAGGCTATTTCCACAGGAAGGCTGCTAATCAGAGAAGGAAAAATACCATTTGTGGGTTATATAATGAAAATGGTAAATGGTGTGATGATGAAAGAGGTATGGAAAATGTAATTACCTCTTATTTCTTTTCTGTGTTCACTGCAACTGACATTGACAATGAAGCCATGAATATTACCTTGGAAGACATTCATCCTTATGTTTCGCATGAAATGAATGAAAACTTGTGCGCACCGTACACTCAAGAGGAGGTGAAGTGTGCTCTATTTCAGATTTATCCAACCAAATCACCGGGGCCGGATGGAATGCCTCCCATATTTTTTCAGCATTACTGGAACACCATTGGGGGAGACATAACTGAGGCTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005488 GO:0006950 GO:0006952 GO:0008150 GO:0016787 GO:0016788 GO:0019863 GO:0019865 GO:0044877 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

181

Amino Acids

21.25

Weight (kDa)

4.98

Isoelectric Point (pI)

47.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 406
AclWI GGATC 1 cut(s) 177
AfaI GTAC 2 cut(s) 150, 413
AfiI CCNNNNNNNGG 2 cut(s) 196, 231
AgsI TTSAA 1 cut(s) 57
AjuI GAANNNNNNNTTGG 2 cut(s) 338, 370
AluBI AGCT 2 cut(s) 37, 126
AluI AGCT 2 cut(s) 37, 126
Alw21I GWGCWC 2 cut(s) 39, 439
Alw26I GTCTC 2 cut(s) 19, 519
AlwI GGATC 1 cut(s) 177
AoxI GGCC 1 cut(s) 470
ApeKI GCWGC 2 cut(s) 100, 200
Asp700I GAANNNNTTC 1 cut(s) 363
AspLEI GCGC 1 cut(s) 407
AspS9I GGNCC 1 cut(s) 470
AsuC2I CCSGG 1 cut(s) 468
AsuHPI GGTGA 2 cut(s) 439, 456
BanII GRGCYC 1 cut(s) 39
BarI GAAGNNNNNNTAC 2 cut(s) 205, 237
BbsI GAAGAC 1 cut(s) 366
Bbv12I GWGCWC 2 cut(s) 39, 439
BbvI GCAGC 2 cut(s) 112, 187
BccI CCATC 2 cut(s) 82, 470
BcnI CCSGG 1 cut(s) 468
BcoDI GTCTC 2 cut(s) 19, 519
BisI GCNGC 2 cut(s) 101, 201
BlsI GCNGC 2 cut(s) 102, 202
Bme1390I CCNGG 1 cut(s) 468
BmgT120I GGNCC 1 cut(s) 470
BmiI GGNNCC 1 cut(s) 471
BmrFI CCNGG 1 cut(s) 468
BpiI GAAGAC 1 cut(s) 366
BpmI CTGGAG 1 cut(s) 50
BpuEI CTTGAG 1 cut(s) 402
BpuMI CCSGG 1 cut(s) 468
BsaJI CCNNGG 2 cut(s) 354, 467
BsaXI ACNNNNNCTCC 2 cut(s) 416, 446
Bsc4I CCNNNNNNNGG 2 cut(s) 196, 231
Bse1I ACTGG 2 cut(s) 33, 512
Bse3DI GCAATG 1 cut(s) 69
BseDI CCNNGG 2 cut(s) 354, 467
BseGI GGATG 2 cut(s) 367, 481
BseLI CCNNNNNNNGG 2 cut(s) 196, 231
BseMI GCAATG 1 cut(s) 69
BseMII CTCAG 1 cut(s) 525
BseNI ACTGG 2 cut(s) 33, 512
BseRI GAGGAG 2 cut(s) 47, 437
BseXI GCAGC 2 cut(s) 112, 187
BshFI GGCC 1 cut(s) 472
BsiHKAI GWGCWC 2 cut(s) 39, 439
BsiSI CCGG 2 cut(s) 467, 473
BslI CCNNNNNNNGG 2 cut(s) 196, 231
BsmAI GTCTC 2 cut(s) 19, 519
BsmI GAATGC 1 cut(s) 486
BsnI GGCC 1 cut(s) 472
Bsp1286I GDGCHC 2 cut(s) 39, 439
Bsp143I GATC 1 cut(s) 169
BspANI GGCC 1 cut(s) 472
BspCNI CTCAG 1 cut(s) 526
BspLI GGNNCC 1 cut(s) 471
BspPI GGATC 1 cut(s) 177
BsrDI GCAATG 1 cut(s) 69
BsrI ACTGG 2 cut(s) 33, 512
BssECI CCNNGG 2 cut(s) 354, 467
BssMI GATC 1 cut(s) 169
BssT1I CCWWGG 1 cut(s) 354
Bst4CI ACNGT 2 cut(s) 84, 411
Bst6I CTCTTC 3 cut(s) 66, 114, 159
BstDEI CTNAG 1 cut(s) 534
BstF5I GGATG 2 cut(s) 367, 481
BstHHI GCGC 1 cut(s) 407
BstKTI GATC 1 cut(s) 172
BstMAI GTCTC 2 cut(s) 19, 519
BstMBI GATC 1 cut(s) 169
BstSCI CCNGG 1 cut(s) 466
BstV1I GCAGC 2 cut(s) 112, 187
BstV2I GAAGAC 1 cut(s) 366
BsuRI GGCC 1 cut(s) 472
BtsCI GGATG 2 cut(s) 367, 481
BtsI GCAGTG 1 cut(s) 315
BtsIMutI CAGTG 1 cut(s) 315
CfoI GCGC 1 cut(s) 407
Cfr13I GGNCC 1 cut(s) 470
Csp6I GTAC 2 cut(s) 149, 412
CviAII CATG 2 cut(s) 343, 383
CviJI RGCY 7 cut(s) 37, 126, 183, 200, 341, 472, 539
CviKI_1 RGCY 7 cut(s) 37, 126, 183, 200, 341, 472, 539
CviQI GTAC 2 cut(s) 149, 412
DdeI CTNAG 1 cut(s) 534
DpnI GATC 1 cut(s) 171
DpnII GATC 1 cut(s) 169
Eam1104I CTCTTC 3 cut(s) 66, 114, 159
EarI CTCTTC 3 cut(s) 66, 114, 159
Ecl136II GAGCTC 1 cut(s) 37
Eco130I CCWWGG 1 cut(s) 354
Eco24I GRGCYC 1 cut(s) 39
Eco53kI GAGCTC 1 cut(s) 37
EcoICRI GAGCTC 1 cut(s) 37
EcoT14I CCWWGG 1 cut(s) 354
EcoT38I GRGCYC 1 cut(s) 39
ErhI CCWWGG 1 cut(s) 354
FaeI CATG 2 cut(s) 346, 386
FaiI YATR 8 cut(s) 238, 240, 278, 344, 375, 384, 491, 530
FatI CATG 2 cut(s) 342, 382
Fnu4HI GCNGC 2 cut(s) 101, 201
FokI GGATG 2 cut(s) 354, 488
FriOI GRGCYC 1 cut(s) 39
Fsp4HI GCNGC 2 cut(s) 101, 201
FspAI RTGCGCAY 1 cut(s) 406
FspI TGCGCA 1 cut(s) 406
GlaI GCGC 1 cut(s) 406
GluI GCNGC 2 cut(s) 101, 201
GsuI CTGGAG 1 cut(s) 50
HaeIII GGCC 1 cut(s) 472
HapII CCGG 2 cut(s) 467, 473
HhaI GCGC 1 cut(s) 407
Hin1II CATG 2 cut(s) 346, 386
Hin6I GCGC 1 cut(s) 405
HinP1I GCGC 1 cut(s) 405
HpaII CCGG 2 cut(s) 467, 473
HphI GGTGA 2 cut(s) 439, 456
Hpy166II GTNNAC 2 cut(s) 315, 414
Hpy188I TCNGA 3 cut(s) 174, 210, 447
Hpy188III TCNNGA 1 cut(s) 419
Hpy8I GTNNAC 2 cut(s) 315, 414
HpyAV CCTTC 2 cut(s) 190, 207
HpyCH4III ACNGT 2 cut(s) 84, 411
HpyCH4V TGCA 4 cut(s) 4, 62, 100, 320
HpyF3I CTNAG 1 cut(s) 534
Hsp92II CATG 2 cut(s) 346, 386
HspAI GCGC 1 cut(s) 405
Kzo9I GATC 1 cut(s) 169
LmnI GCTCC 1 cut(s) 34
LpnPI CCDG 6 cut(s) 14, 165, 178, 480, 486, 493
Lsp1109I GCAGC 2 cut(s) 112, 187
MalI GATC 1 cut(s) 171
MboI GATC 1 cut(s) 169
MboII GAAGA 5 cut(s) 83, 88, 131, 176, 371
MhlI GDGCHC 2 cut(s) 39, 439
MluCI AATT 1 cut(s) 288
MmeI TCCRAC 1 cut(s) 479
MnlI CCTC 9 cut(s) 25, 85, 160, 266, 304, 415, 418, 495, 529
MroXI GAANNNNTTC 1 cut(s) 363
MseI TTAA 1 cut(s) 158
MslI CAYNNNNRTG 1 cut(s) 372
MspI CCGG 2 cut(s) 467, 473
MspR9I CCNGG 1 cut(s) 468
Mva1269I GAATGC 1 cut(s) 486
NciI CCSGG 1 cut(s) 468
NdeII GATC 1 cut(s) 169
NlaIII CATG 2 cut(s) 346, 386
NlaIV GGNNCC 1 cut(s) 471
NsbI TGCGCA 1 cut(s) 406
PctI GAATGC 1 cut(s) 486
PdmI GAANNNNTTC 1 cut(s) 363
PkrI GCNGC 2 cut(s) 102, 202
Psp124BI GAGCTC 1 cut(s) 39
PspN4I GGNNCC 1 cut(s) 471
PspPI GGNCC 1 cut(s) 470
RsaI GTAC 2 cut(s) 150, 413
RsaNI GTAC 2 cut(s) 149, 412
RseI CAYNNNNRTG 1 cut(s) 372
SacI GAGCTC 1 cut(s) 39
SaqAI TTAA 1 cut(s) 158
SatI GCNGC 2 cut(s) 101, 201
Sau3AI GATC 1 cut(s) 169
Sau96I GGNCC 1 cut(s) 470
ScrFI CCNGG 1 cut(s) 468
SduI GDGCHC 2 cut(s) 39, 439
SetI ASST 6 cut(s) 39, 128, 277, 296, 356, 429
SmiMI CAYNNNNRTG 1 cut(s) 372
SmlI CTYRAG 1 cut(s) 417
SmoI CTYRAG 1 cut(s) 417
Sse9I AATT 1 cut(s) 288
SspI AATATT 1 cut(s) 349
SstI GAGCTC 1 cut(s) 39
StyD4I CCNGG 1 cut(s) 466
StyI CCWWGG 1 cut(s) 354
TaaI ACNGT 2 cut(s) 84, 411
TasI AATT 1 cut(s) 288
TatI WGTACW 1 cut(s) 148
Tru1I TTAA 1 cut(s) 158
Tru9I TTAA 1 cut(s) 158
TscAI CASTG 1 cut(s) 322
TseI GCWGC 2 cut(s) 100, 200
TspDTI ATGAA 8 cut(s) 258, 282, 351, 356, 359, 399, 404, 408
TspRI CASTG 1 cut(s) 322
XmnI GAANNNNTTC 1 cut(s) 363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.