RLG00000011782

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000978
Physical Location & Seq
Reverse (-)
12625807 .. 12627420
1614 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011782

Sequence Viewer

Length: 942 bp
ATGCCGGACGTATTTTGTACTGTATTGATGAGACTAACTTGTGGTTCAGCAAAGAGTCTTGATCACCCTTATCTAAGCACATATCTGGATTCCCTTGGGACAAATTTCTCGCATGGTGCAAATTTCGCCACTGCAGCTTCCACTATCAGACTCCCAGATCTCATTATGCCAGCCGGCGGTGGATATAGCCCCTTCTACCTTGATATTCAATATGTGCAGTTCCTGCAACTAAAATCCAGATCACAACTCATAAGGCAACGAGGGGGAATATTTGCAAGTTTAATGCCCAAGGAGGAGTACTTCTCCAAAGCTTTATACACATTCGACATTGGTCAGAATGATCTTGGCGAGGGTTTCGGTAACAAGACTATTCAGGAAGTCAATGCATCTGTTCCTGATATAATCACTGGTTTCTCAACTAATATCAAGCAAGATCATTATGGATCCACAATACGGGGCCGATTGGCTGTCTCCCTTACATTGCGGCAAATTAACTTCCCAGCTGCTGAGAAAGATGAGATTGGCTGTGCAAAGGCTTACAATGACGTAGCTCAACATTTTAACCACGAGCTGAAGCAGGCCATAGTTCAACTCAGGAAGGATCTTCCTTTGGCTGCATTCACTTATGTAGATGTGTATTCTGTCAAATACTCTCTTTTCAAGGAACCCGAGAAGTATGGATTTGAGCTCCCACTTGTTGCTTGTTGTGGCTACGGTGGTAAGTACAACTATAACGCCACTGCTGAATGTGGAGCAACAATTACAGTCAATGGAAGCCAAATCTTTGTTGGTTCATGCAAAAACCCCTCAACTAGAGTGACCTGGGATGGGGGTCATTACACCGAGGCAGCTGCGAAATTTGTTTTTGATAAAATTTCAACTGGAGCATATTCAGATCCACCTCTTCCCTTGAAACAAGCATGTCACAGGAGTTTGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005488 GO:0006950 GO:0006952 GO:0008150 GO:0016787 GO:0016788 GO:0019863 GO:0019865 GO:0044877 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

314

Amino Acids

34.47

Weight (kDa)

7.56

Isoelectric Point (pI)

40.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 23 - 292 4.3e-27 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 177, 484
AclWI GGATC 4 cut(s) 438, 451, 609, 890
AcsI RAATTY 4 cut(s) 103, 121, 857, 873
AcuI CTGAAG 1 cut(s) 593
AfaI GTAC 3 cut(s) 19, 299, 725
AfiI CCNNNNNNNGG 3 cut(s) 176, 453, 828
AgsI TTSAA 6 cut(s) 209, 590, 661, 879, 913, 937
AjnI CCWGG 1 cut(s) 821
AluBI AGCT 7 cut(s) 137, 311, 503, 551, 571, 688, 851
AluI AGCT 7 cut(s) 137, 311, 503, 551, 571, 688, 851
Alw21I GWGCWC 1 cut(s) 690
Alw26I GTCTC 2 cut(s) 25, 475
AlwI GGATC 4 cut(s) 438, 451, 609, 890
AlwNI CAGNNNCTG 2 cut(s) 223, 506
Ama87I CYCGRG 1 cut(s) 668
AoxI GGCC 2 cut(s) 457, 579
ApeKI GCWGC 5 cut(s) 134, 503, 614, 848, 851
ApoI RAATTY 4 cut(s) 103, 121, 857, 873
AspS9I GGNCC 1 cut(s) 457
AsuHPI GGTGA 1 cut(s) 56
AvaI CYCGRG 1 cut(s) 668
BamHI GGATCC 1 cut(s) 443
BanII GRGCYC 1 cut(s) 690
BauI CACGAG 1 cut(s) 566
Bbv12I GWGCWC 1 cut(s) 690
BbvI GCAGC 5 cut(s) 146, 490, 601, 838, 860
BccI CCATC 1 cut(s) 821
BcgI CGANNNNNNTGC 2 cut(s) 833, 867
BciT130I CCWGG 1 cut(s) 823
BclI TGATCA 1 cut(s) 61
BcoDI GTCTC 2 cut(s) 25, 475
BfaI CTAG 1 cut(s) 813
BfmI CTRYAG 1 cut(s) 132
BglII AGATCT 1 cut(s) 157
BisI GCNGC 6 cut(s) 135, 485, 504, 615, 849, 852
BlsI GCNGC 6 cut(s) 136, 486, 505, 616, 850, 853
BmcAI AGTACT 1 cut(s) 299
Bme1390I CCNGG 1 cut(s) 823
BmeT110I CYCGRG 1 cut(s) 668
BmgT120I GGNCC 1 cut(s) 457
BmiI GGNNCC 3 cut(s) 445, 458, 666
BmrFI CCNGG 1 cut(s) 823
BmsI GCATC 1 cut(s) 395
BoxI GACNNNNGTC 1 cut(s) 330
BplI GAGNNNNNCTC 2 cut(s) 287, 319
BpmI CTGGAG 1 cut(s) 903
BsaJI CCNNGG 4 cut(s) 94, 288, 822, 843
Bsc4I CCNNNNNNNGG 3 cut(s) 176, 453, 828
Bse118I RCCGGY 1 cut(s) 173
Bse1I ACTGG 2 cut(s) 412, 886
Bse3DI GCAATG 1 cut(s) 479
BseBI CCWGG 1 cut(s) 823
BseDI CCNNGG 4 cut(s) 94, 288, 822, 843
BseGI GGATG 1 cut(s) 832
BseLI CCNNNNNNNGG 3 cut(s) 176, 453, 828
BseMI GCAATG 1 cut(s) 479
BseMII CTCAG 2 cut(s) 498, 607
BseNI ACTGG 2 cut(s) 412, 886
BseRI GAGGAG 1 cut(s) 308
BseXI GCAGC 5 cut(s) 146, 490, 601, 838, 860
BseYI CCCAGC 1 cut(s) 499
BsgI GTGCAG 1 cut(s) 236
BshFI GGCC 2 cut(s) 459, 581
BsiHKAI GWGCWC 1 cut(s) 690
BsiHKCI CYCGRG 1 cut(s) 668
BsiSI CCGG 2 cut(s) 5, 174
BslFI GGGAC 1 cut(s) 112
BslI CCNNNNNNNGG 3 cut(s) 176, 453, 828
BsmAI GTCTC 2 cut(s) 25, 475
BsmFI GGGAC 1 cut(s) 112
BsmI GAATGC 1 cut(s) 617
BsnI GGCC 2 cut(s) 459, 581
BsoBI CYCGRG 1 cut(s) 668
Bsp1286I GDGCHC 1 cut(s) 690
Bsp143I GATC 8 cut(s) 61, 157, 239, 340, 433, 443, 601, 895
BspACI CCGC 2 cut(s) 177, 484
BspANI GGCC 2 cut(s) 459, 581
BspCNI CTCAG 2 cut(s) 499, 606
BspLI GGNNCC 3 cut(s) 445, 458, 666
BspMAI CTGCAG 1 cut(s) 136
BspPI GGATC 4 cut(s) 438, 451, 609, 890
BsrDI GCAATG 1 cut(s) 479
BsrFI RCCGGY 1 cut(s) 173
BsrI ACTGG 2 cut(s) 412, 886
BssAI RCCGGY 1 cut(s) 173
BssECI CCNNGG 4 cut(s) 94, 288, 822, 843
BssMI GATC 8 cut(s) 61, 157, 239, 340, 433, 443, 601, 895
BssSI CACGAG 1 cut(s) 566
BssT1I CCWWGG 2 cut(s) 94, 288
Bst2BI CACGAG 1 cut(s) 566
Bst2UI CCWGG 1 cut(s) 823
Bst4CI ACNGT 3 cut(s) 22, 716, 766
Bst6I CTCTTC 1 cut(s) 909
BstAPI GCANNNNNTGC 1 cut(s) 223
BstC8I GCNNGC 3 cut(s) 171, 175, 579
BstDEI CTNAG 3 cut(s) 74, 507, 593
BstF5I GGATG 1 cut(s) 832
BstKTI GATC 8 cut(s) 64, 160, 242, 343, 436, 446, 604, 898
BstMAI GTCTC 2 cut(s) 25, 475
BstMBI GATC 8 cut(s) 61, 157, 239, 340, 433, 443, 601, 895
BstMWI GCNNNNNNNGC 3 cut(s) 125, 134, 223
BstNI CCWGG 1 cut(s) 823
BstNSI RCATGY 1 cut(s) 924
BstPAI GACNNNNGTC 1 cut(s) 330
BstSCI CCNGG 1 cut(s) 821
BstSFI CTRYAG 1 cut(s) 132
BstV1I GCAGC 5 cut(s) 146, 490, 601, 838, 860
BstX2I RGATCY 4 cut(s) 157, 443, 601, 895
BstYI RGATCY 4 cut(s) 157, 443, 601, 895
BsuRI GGCC 2 cut(s) 459, 581
BtsCI GGATG 1 cut(s) 832
BtsI GCAGTG 2 cut(s) 129, 738
BtsIMutI CAGTG 3 cut(s) 129, 405, 738
Cac8I GCNNGC 3 cut(s) 171, 175, 579
CaiI CAGNNNCTG 2 cut(s) 223, 506
Cfr10I RCCGGY 1 cut(s) 173
Cfr13I GGNCC 1 cut(s) 457
Csp6I GTAC 3 cut(s) 18, 298, 724
CviAII CATG 3 cut(s) 113, 795, 921
CviQI GTAC 3 cut(s) 18, 298, 724
DdeI CTNAG 3 cut(s) 74, 507, 593
DpnI GATC 8 cut(s) 63, 159, 241, 342, 435, 445, 603, 897
DpnII GATC 8 cut(s) 61, 157, 239, 340, 433, 443, 601, 895
Eam1104I CTCTTC 1 cut(s) 909
EarI CTCTTC 1 cut(s) 909
Ecl136II GAGCTC 1 cut(s) 688
Eco130I CCWWGG 2 cut(s) 94, 288
Eco24I GRGCYC 1 cut(s) 690
Eco53kI GAGCTC 1 cut(s) 688
Eco57I CTGAAG 1 cut(s) 593
Eco88I CYCGRG 1 cut(s) 668
EcoICRI GAGCTC 1 cut(s) 688
EcoRII CCWGG 1 cut(s) 821
EcoT14I CCWWGG 2 cut(s) 94, 288
EcoT22I ATGCAT 1 cut(s) 388
EcoT38I GRGCYC 1 cut(s) 690
ErhI CCWWGG 2 cut(s) 94, 288
FaeI CATG 3 cut(s) 116, 798, 924
FaqI GGGAC 1 cut(s) 112
FatI CATG 3 cut(s) 112, 794, 920
FbaI TGATCA 1 cut(s) 61
Fnu4HI GCNGC 6 cut(s) 135, 485, 504, 615, 849, 852
FokI GGATG 1 cut(s) 839
FriOI GRGCYC 1 cut(s) 690
Fsp4HI GCNGC 6 cut(s) 135, 485, 504, 615, 849, 852
FspBI CTAG 1 cut(s) 813
GluI GCNGC 6 cut(s) 135, 485, 504, 615, 849, 852
GsaI CCCAGC 1 cut(s) 503
GsuI CTGGAG 1 cut(s) 903
HaeIII GGCC 2 cut(s) 459, 581
HapII CCGG 2 cut(s) 5, 174
Hin1II CATG 3 cut(s) 116, 798, 924
HindIII AAGCTT 1 cut(s) 309
HinfI GANTC 3 cut(s) 55, 89, 150
HpaII CCGG 2 cut(s) 5, 174
HphI GGTGA 1 cut(s) 56
Hpy188I TCNGA 3 cut(s) 149, 336, 895
Hpy188III TCNNGA 6 cut(s) 59, 86, 237, 374, 395, 595
HpyAV CCTTC 2 cut(s) 202, 592
HpyCH4III ACNGT 3 cut(s) 22, 716, 766
HpyCH4IV ACGT 2 cut(s) 9, 546
HpyCH4V TGCA 9 cut(s) 119, 134, 217, 226, 275, 386, 530, 617, 798
HpyF10VI GCNNNNNNNGC 3 cut(s) 125, 134, 223
HpyF3I CTNAG 3 cut(s) 74, 507, 593
HpySE526I ACGT 2 cut(s) 9, 546
Hsp92II CATG 3 cut(s) 116, 798, 924
KroI GCCGGC 1 cut(s) 173
KroNI GCCGGC 1 cut(s) 175
Ksp22I TGATCA 1 cut(s) 61
Kzo9I GATC 8 cut(s) 61, 157, 239, 340, 433, 443, 601, 895
LmnI GCTCC 3 cut(s) 693, 752, 884
Lsp1109I GCAGC 5 cut(s) 146, 490, 601, 838, 860
LweI GCATC 1 cut(s) 395
MaeI CTAG 1 cut(s) 813
MaeII ACGT 2 cut(s) 9, 546
MaeIII GTNAC 3 cut(s) 359, 817, 923
MalI GATC 8 cut(s) 63, 159, 241, 342, 435, 445, 603, 897
MboI GATC 8 cut(s) 61, 157, 239, 340, 433, 443, 601, 895
MboII GAAGA 2 cut(s) 596, 896
MflI RGATCY 4 cut(s) 157, 443, 601, 895
MhlI GDGCHC 1 cut(s) 690
MluCI AATT 6 cut(s) 103, 121, 489, 759, 857, 873
MlyI GAGTC 2 cut(s) 64, 144
MnlI CCTC 6 cut(s) 254, 286, 343, 817, 838, 912
Mph1103I ATGCAT 1 cut(s) 388
MroNI GCCGGC 1 cut(s) 173
MseI TTAA 3 cut(s) 281, 492, 561
MspA1I CMGCKG 2 cut(s) 503, 851
MspI CCGG 2 cut(s) 5, 174
MspR9I CCNGG 1 cut(s) 823
Mva1269I GAATGC 1 cut(s) 617
MvaI CCWGG 1 cut(s) 823
MwoI GCNNNNNNNGC 3 cut(s) 125, 134, 223
NaeI GCCGGC 1 cut(s) 175
NdeII GATC 8 cut(s) 61, 157, 239, 340, 433, 443, 601, 895
NgoMIV GCCGGC 1 cut(s) 173
NlaIII CATG 3 cut(s) 116, 798, 924
NlaIV GGNNCC 3 cut(s) 445, 458, 666
NmuCI GTSAC 2 cut(s) 817, 923
NsiI ATGCAT 1 cut(s) 388
NspI RCATGY 1 cut(s) 924
PctI GAATGC 1 cut(s) 617
PdiI GCCGGC 1 cut(s) 175
PfeI GAWTC 1 cut(s) 89
PkrI GCNGC 6 cut(s) 136, 486, 505, 616, 850, 853
PleI GAGTC 2 cut(s) 63, 144
PpsI GAGTC 2 cut(s) 63, 144
PshAI GACNNNNGTC 1 cut(s) 330
Psp124BI GAGCTC 1 cut(s) 690
Psp6I CCWGG 1 cut(s) 821
PspFI CCCAGC 1 cut(s) 499
PspGI CCWGG 1 cut(s) 821
PspN4I GGNNCC 3 cut(s) 445, 458, 666
PspPI GGNCC 1 cut(s) 457
PstI CTGCAG 1 cut(s) 136
PstNI CAGNNNCTG 2 cut(s) 223, 506
PsuI RGATCY 4 cut(s) 157, 443, 601, 895
PvuII CAGCTG 2 cut(s) 503, 851
RsaI GTAC 3 cut(s) 19, 299, 725
RsaNI GTAC 3 cut(s) 18, 298, 724
SacI GAGCTC 1 cut(s) 690
SaqAI TTAA 3 cut(s) 281, 492, 561
SatI GCNGC 6 cut(s) 135, 485, 504, 615, 849, 852
Sau3AI GATC 8 cut(s) 61, 157, 239, 340, 433, 443, 601, 895
Sau96I GGNCC 1 cut(s) 457
ScaI AGTACT 1 cut(s) 299
SchI GAGTC 2 cut(s) 64, 144
ScrFI CCNGG 1 cut(s) 823
SduI GDGCHC 1 cut(s) 690
SfaNI GCATC 1 cut(s) 395
SfcI CTRYAG 1 cut(s) 132
Sse9I AATT 6 cut(s) 103, 121, 489, 759, 857, 873
SsiI CCGC 2 cut(s) 177, 484
SspI AATATT 1 cut(s) 270
SspMI CTAG 1 cut(s) 813
SstI GAGCTC 1 cut(s) 690
StyD4I CCNGG 1 cut(s) 821
StyI CCWWGG 2 cut(s) 94, 288
TaaI ACNGT 3 cut(s) 22, 716, 766
TaiI ACGT 2 cut(s) 12, 549
TaqI TCGA 1 cut(s) 324
TasI AATT 6 cut(s) 103, 121, 489, 759, 857, 873
TatI WGTACW 3 cut(s) 17, 297, 723
TauI GCSGC 1 cut(s) 487
TfiI GAWTC 1 cut(s) 89
Tru1I TTAA 3 cut(s) 281, 492, 561
Tru9I TTAA 3 cut(s) 281, 492, 561
TscAI CASTG 3 cut(s) 136, 412, 745
TseFI GTSAC 2 cut(s) 817, 923
TseI GCWGC 5 cut(s) 134, 503, 614, 848, 851
Tsp45I GTSAC 2 cut(s) 817, 923
TspDTI ATGAA 1 cut(s) 783
TspRI CASTG 3 cut(s) 136, 412, 745
XapI RAATTY 4 cut(s) 103, 121, 857, 873
XceI RCATGY 1 cut(s) 924
XcmI CCANNNNNNNNNTGG 1 cut(s) 785
XspI CTAG 1 cut(s) 813
ZrmI AGTACT 1 cut(s) 299
Zsp2I ATGCAT 1 cut(s) 388
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.