RLG00000011766

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000978
Physical Location & Seq
Forward (+)
12445715 .. 12448049
2335 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011766

Sequence Viewer

Length: 1167 bp
ATGGGGTACTCTCCTAAAGTTATCATTTCTCTGTTCTGCTCCTATATGCTTATATTGCATATTACCAGTAGTGCTGTGATCCCTACCTTGGCTTTGAAAGACTGTGAGTTTCCTGCCATCTTCAACTTCGGAGACTCAAATTCAGATACTGGTGGACTAGCCGCATCCCTTCTACCACCGACCACACCTTATGGAGAGACCTATTTCCATATGCCGGCCGGAAGATTCTCTGATGGAAGACTCATCATCGATTTCCTTGCAAAGAGTCTTGGTCACCCTTATCTAAGCGCATATCTGAATTCTCTCGGGACAAATTTCTCGCATGGTGCAAATTTCGCCACTGCCGCTTCCACTATCAGACTCCCAGATTCCATTTTACCAGCCGGTGAATTTAGTCCCTTCTACCTTGATATTCAATATATGCAGTTCCTGCAACTAAAATCCAGATCACAACTCATAAGGCATCGAGGGGGAATATTTGCAAGTTTAATGCCCAAGGAGGAGTACTTCTCCAAAGCTTTATACACATTCGACATTGGTCAGAATGATCTTGGCGAGGGATTTTTCGGTAACATGACTATACAGGAAGTAAATGCATCTGTTCCTGATATAATCACTGGTTTCTCAACTAATATCAAGAAAATATATGATTTGGGAGCAAGATCATTATGGATCCACAATACGGGGCCGATTGGATGTCTCCCTTACATTGTGGCAGACTTCCCGTCAGCTGAGAAAGATGAGATTGGCTGTGCAAAGGCTTACAATGAAGTAGCTCAACATTTTAACCACGAGCTGAAGCAGGCCATAGTTCAACTCAGAAAAGATCTTCCTTTGGCTGCATTCACTTATGTAGATGTGTACTCTGTCAAATACTCTCTTTTCAAGGAACCCGAAAAGTATGGATTTGAGCTCCCACTTGTTGCTTGTTGTGGCTCCGGTGGTAAGTACAACTATAACGCCACTGCTGGATGTGGAGCAATAATTACAGTCAATGGAAGCCAAATCCTTGTTGGTTCATGCAAAAACCCCTCAATTAGAGTGAACTGGGATGGTGTTCATTACACCGAGGCAGCAGCGAAATTTGTTTTTGATAAAATTTCAACTGGAGCGTATTCAGATCCACCTCTTCCCTTGAAACAAGCATGTCACAGGAGTTTGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005488 GO:0006950 GO:0006952 GO:0008150 GO:0016787 GO:0016788 GO:0019863 GO:0019865 GO:0044877 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

389

Amino Acids

42.51

Weight (kDa)

6.36

Isoelectric Point (pI)

38.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 40 - 367 1.4e-47 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 162, 345
AclWI GGATC 4 cut(s) 73, 667, 680, 1115
AcoI YGGCCR 1 cut(s) 216
AcsI RAATTY 7 cut(s) 139, 298, 313, 331, 389, 1082, 1098
AcuI CTGAAG 1 cut(s) 818
AfaI GTAC 4 cut(s) 8, 506, 863, 950
AfiI CCNNNNNNNGG 3 cut(s) 88, 214, 682
AgsI TTSAA 8 cut(s) 97, 124, 416, 815, 886, 1104, 1138, 1162
AhdI GACNNNNNGTC 1 cut(s) 724
AluBI AGCT 5 cut(s) 518, 731, 776, 796, 913
AluI AGCT 5 cut(s) 518, 731, 776, 796, 913
Alw21I GWGCWC 1 cut(s) 915
Alw26I GTCTC 3 cut(s) 126, 191, 704
AlwI GGATC 4 cut(s) 73, 667, 680, 1115
AlwNI CAGNNNCTG 2 cut(s) 149, 430
Ama87I CYCGRG 1 cut(s) 305
AoxI GGCC 3 cut(s) 216, 686, 804
ApeKI GCWGC 3 cut(s) 839, 1073, 1076
ApoI RAATTY 7 cut(s) 139, 298, 313, 331, 389, 1082, 1098
AspLEI GCGC 1 cut(s) 290
AspS9I GGNCC 1 cut(s) 686
AsuHPI GGTGA 2 cut(s) 266, 398
AvaI CYCGRG 1 cut(s) 305
BamHI GGATCC 1 cut(s) 672
BanII GRGCYC 1 cut(s) 915
BauI CACGAG 1 cut(s) 791
BbsI GAAGAC 1 cut(s) 244
Bbv12I GWGCWC 1 cut(s) 915
BbvI GCAGC 3 cut(s) 826, 1085, 1088
BccI CCATC 3 cut(s) 125, 227, 1046
BcgI CGANNNNNNTGC 2 cut(s) 239, 273
BcoDI GTCTC 3 cut(s) 126, 191, 704
BfaI CTAG 1 cut(s) 158
BglII AGATCT 1 cut(s) 826
BisI GCNGC 5 cut(s) 162, 345, 840, 1074, 1077
BlsI GCNGC 5 cut(s) 163, 346, 841, 1075, 1078
BmcAI AGTACT 1 cut(s) 506
BmeRI GACNNNNNGTC 1 cut(s) 724
BmeT110I CYCGRG 1 cut(s) 305
BmgT120I GGNCC 1 cut(s) 686
BmiI GGNNCC 4 cut(s) 674, 687, 891, 937
BmrI ACTGGG 1 cut(s) 1057
BmsI GCATC 3 cut(s) 173, 472, 605
BmuI ACTGGG 1 cut(s) 1057
BoxI GACNNNNGTC 1 cut(s) 537
BpiI GAAGAC 1 cut(s) 244
BplI GAGNNNNNCTC 2 cut(s) 494, 526
BpmI CTGGAG 1 cut(s) 1128
Bsa29I ATCGAT 1 cut(s) 249
BsaI GGTCTC 1 cut(s) 191
BsaJI CCNNGG 3 cut(s) 87, 495, 1068
BsaWI WCCGGW 1 cut(s) 938
Bsc4I CCNNNNNNNGG 3 cut(s) 88, 214, 682
Bse118I RCCGGY 2 cut(s) 214, 383
Bse1I ACTGG 5 cut(s) 66, 154, 622, 1052, 1111
BseCI ATCGAT 1 cut(s) 249
BseDI CCNNGG 3 cut(s) 87, 495, 1068
BseGI GGATG 4 cut(s) 164, 701, 977, 1057
BseLI CCNNNNNNNGG 3 cut(s) 88, 214, 682
BseMII CTCAG 2 cut(s) 723, 832
BseNI ACTGG 5 cut(s) 66, 154, 622, 1052, 1111
BseRI GAGGAG 1 cut(s) 515
BseX3I CGGCCG 1 cut(s) 216
BseXI GCAGC 3 cut(s) 826, 1085, 1088
Bsh1285I CGRYCG 1 cut(s) 219
BshFI GGCC 3 cut(s) 218, 688, 806
BshVI ATCGAT 1 cut(s) 249
BsiEI CGRYCG 1 cut(s) 219
BsiHKAI GWGCWC 1 cut(s) 915
BsiHKCI CYCGRG 1 cut(s) 305
BsiSI CCGG 4 cut(s) 215, 219, 384, 939
BslFI GGGAC 2 cut(s) 322, 381
BslI CCNNNNNNNGG 3 cut(s) 88, 214, 682
BsmAI GTCTC 3 cut(s) 126, 191, 704
BsmFI GGGAC 2 cut(s) 322, 381
BsmI GAATGC 1 cut(s) 842
BsnI GGCC 3 cut(s) 218, 688, 806
Bso31I GGTCTC 1 cut(s) 191
BsoBI CYCGRG 1 cut(s) 305
Bsp1286I GDGCHC 1 cut(s) 915
Bsp143I GATC 7 cut(s) 78, 446, 547, 662, 672, 826, 1120
BspACI CCGC 2 cut(s) 162, 345
BspANI GGCC 3 cut(s) 218, 688, 806
BspCNI CTCAG 2 cut(s) 724, 831
BspDI ATCGAT 1 cut(s) 249
BspLI GGNNCC 4 cut(s) 674, 687, 891, 937
BspPI GGATC 4 cut(s) 73, 667, 680, 1115
BspTNI GGTCTC 1 cut(s) 191
BsrFI RCCGGY 2 cut(s) 214, 383
BsrI ACTGG 5 cut(s) 66, 154, 622, 1052, 1111
BssAI RCCGGY 2 cut(s) 214, 383
BssECI CCNNGG 3 cut(s) 87, 495, 1068
BssMI GATC 7 cut(s) 78, 446, 547, 662, 672, 826, 1120
BssSI CACGAG 1 cut(s) 791
BssT1I CCWWGG 2 cut(s) 87, 495
Bst2BI CACGAG 1 cut(s) 791
Bst4CI ACNGT 2 cut(s) 104, 991
Bst6I CTCTTC 1 cut(s) 1134
BstAPI GCANNNNNTGC 1 cut(s) 430
BstC8I GCNNGC 2 cut(s) 216, 804
BstDEI CTNAG 3 cut(s) 284, 732, 818
BstEII GGTNACC 1 cut(s) 272
BstF5I GGATG 4 cut(s) 164, 701, 977, 1057
BstHHI GCGC 1 cut(s) 290
BstKTI GATC 7 cut(s) 81, 449, 550, 665, 675, 829, 1123
BstMAI GTCTC 3 cut(s) 126, 191, 704
BstMBI GATC 7 cut(s) 78, 446, 547, 662, 672, 826, 1120
BstMCI CGRYCG 1 cut(s) 219
BstMWI GCNNNNNNNGC 4 cut(s) 55, 335, 344, 430
BstNSI RCATGY 1 cut(s) 1149
BstPAI GACNNNNGTC 1 cut(s) 537
BstPI GGTNACC 1 cut(s) 272
BstV1I GCAGC 3 cut(s) 826, 1085, 1088
BstV2I GAAGAC 1 cut(s) 244
BstX2I RGATCY 3 cut(s) 672, 826, 1120
BstYI RGATCY 3 cut(s) 672, 826, 1120
BstZI CGGCCG 1 cut(s) 216
Bsu15I ATCGAT 1 cut(s) 249
BsuRI GGCC 3 cut(s) 218, 688, 806
BsuTUI ATCGAT 1 cut(s) 249
BtsCI GGATG 4 cut(s) 164, 701, 977, 1057
BtsI GCAGTG 2 cut(s) 339, 963
BtsIMutI CAGTG 3 cut(s) 339, 615, 963
Cac8I GCNNGC 2 cut(s) 216, 804
CaiI CAGNNNCTG 2 cut(s) 149, 430
CfoI GCGC 1 cut(s) 290
Cfr10I RCCGGY 2 cut(s) 214, 383
Cfr13I GGNCC 1 cut(s) 686
ClaI ATCGAT 1 cut(s) 249
Csp6I GTAC 4 cut(s) 7, 505, 862, 949
CviAII CATG 4 cut(s) 323, 574, 1020, 1146
CviQI GTAC 4 cut(s) 7, 505, 862, 949
DdeI CTNAG 3 cut(s) 284, 732, 818
DpnI GATC 7 cut(s) 80, 448, 549, 664, 674, 828, 1122
DpnII GATC 7 cut(s) 78, 446, 547, 662, 672, 826, 1120
DriI GACNNNNNGTC 1 cut(s) 724
EaeI YGGCCR 1 cut(s) 216
EagI CGGCCG 1 cut(s) 216
Eam1104I CTCTTC 1 cut(s) 1134
Eam1105I GACNNNNNGTC 1 cut(s) 724
EarI CTCTTC 1 cut(s) 1134
Ecl136II GAGCTC 1 cut(s) 913
EclXI CGGCCG 1 cut(s) 216
Eco130I CCWWGG 2 cut(s) 87, 495
Eco24I GRGCYC 1 cut(s) 915
Eco31I GGTCTC 1 cut(s) 191
Eco52I CGGCCG 1 cut(s) 216
Eco53kI GAGCTC 1 cut(s) 913
Eco57I CTGAAG 1 cut(s) 818
Eco88I CYCGRG 1 cut(s) 305
Eco91I GGTNACC 1 cut(s) 272
EcoICRI GAGCTC 1 cut(s) 913
EcoO65I GGTNACC 1 cut(s) 272
EcoRI GAATTC 1 cut(s) 298
EcoT14I CCWWGG 2 cut(s) 87, 495
EcoT22I ATGCAT 1 cut(s) 598
EcoT38I GRGCYC 1 cut(s) 915
ErhI CCWWGG 2 cut(s) 87, 495
FaeI CATG 4 cut(s) 326, 577, 1023, 1149
FaqI GGGAC 2 cut(s) 322, 381
FatI CATG 4 cut(s) 322, 573, 1019, 1145
FauNDI CATATG 1 cut(s) 210
Fnu4HI GCNGC 5 cut(s) 162, 345, 840, 1074, 1077
FokI GGATG 4 cut(s) 151, 708, 984, 1064
FriOI GRGCYC 1 cut(s) 915
Fsp4HI GCNGC 5 cut(s) 162, 345, 840, 1074, 1077
FspBI CTAG 1 cut(s) 158
GlaI GCGC 1 cut(s) 289
GluI GCNGC 5 cut(s) 162, 345, 840, 1074, 1077
GsuI CTGGAG 1 cut(s) 1128
HaeIII GGCC 3 cut(s) 218, 688, 806
HapII CCGG 4 cut(s) 215, 219, 384, 939
HhaI GCGC 1 cut(s) 290
Hin1II CATG 4 cut(s) 326, 577, 1023, 1149
Hin6I GCGC 1 cut(s) 288
HinP1I GCGC 1 cut(s) 288
HindIII AAGCTT 1 cut(s) 516
HinfI GANTC 6 cut(s) 134, 225, 240, 265, 360, 368
HpaII CCGG 4 cut(s) 215, 219, 384, 939
HphI GGTGA 2 cut(s) 266, 398
Hpy166II GTNNAC 3 cut(s) 155, 862, 1045
Hpy188I TCNGA 8 cut(s) 131, 145, 232, 297, 359, 543, 821, 1120
Hpy188III TCNNGA 4 cut(s) 307, 444, 605, 637
Hpy8I GTNNAC 3 cut(s) 155, 862, 1045
HpyAV CCTTC 2 cut(s) 179, 409
HpyCH4III ACNGT 2 cut(s) 104, 991
HpyF10VI GCNNNNNNNGC 4 cut(s) 55, 335, 344, 430
HpyF3I CTNAG 3 cut(s) 284, 732, 818
Hsp92II CATG 4 cut(s) 326, 577, 1023, 1149
HspAI GCGC 1 cut(s) 288
KroI GCCGGC 1 cut(s) 214
KroNI GCCGGC 1 cut(s) 216
Kzo9I GATC 7 cut(s) 78, 446, 547, 662, 672, 826, 1120
LmnI GCTCC 6 cut(s) 44, 656, 918, 941, 977, 1109
Lsp1109I GCAGC 3 cut(s) 826, 1085, 1088
LweI GCATC 3 cut(s) 173, 472, 605
MaeI CTAG 1 cut(s) 158
MaeIII GTNAC 3 cut(s) 272, 569, 1148
MalI GATC 7 cut(s) 80, 448, 549, 664, 674, 828, 1122
MboI GATC 7 cut(s) 78, 446, 547, 662, 672, 826, 1120
MboII GAAGA 5 cut(s) 112, 234, 249, 821, 1121
MflI RGATCY 3 cut(s) 672, 826, 1120
MhlI GDGCHC 1 cut(s) 915
MluCI AATT 9 cut(s) 139, 298, 313, 331, 389, 984, 1035, 1082, 1098
MlyI GAGTC 4 cut(s) 128, 234, 274, 354
MnlI CCTC 6 cut(s) 461, 493, 550, 1042, 1063, 1137
Mph1103I ATGCAT 1 cut(s) 598
MroNI GCCGGC 1 cut(s) 214
MseI TTAA 2 cut(s) 488, 786
MspA1I CMGCKG 1 cut(s) 731
MspI CCGG 4 cut(s) 215, 219, 384, 939
Mva1269I GAATGC 1 cut(s) 842
MwoI GCNNNNNNNGC 4 cut(s) 55, 335, 344, 430
NaeI GCCGGC 1 cut(s) 216
NdeI CATATG 1 cut(s) 210
NdeII GATC 7 cut(s) 78, 446, 547, 662, 672, 826, 1120
NgoMIV GCCGGC 1 cut(s) 214
NlaIII CATG 4 cut(s) 326, 577, 1023, 1149
NlaIV GGNNCC 4 cut(s) 674, 687, 891, 937
NmuCI GTSAC 2 cut(s) 272, 1148
NsiI ATGCAT 1 cut(s) 598
NspI RCATGY 1 cut(s) 1149
PctI GAATGC 1 cut(s) 842
PdiI GCCGGC 1 cut(s) 216
PfeI GAWTC 2 cut(s) 225, 368
PkrI GCNGC 5 cut(s) 163, 346, 841, 1075, 1078
PleI GAGTC 4 cut(s) 128, 234, 273, 354
PpsI GAGTC 4 cut(s) 128, 234, 273, 354
PshAI GACNNNNGTC 1 cut(s) 537
Psp124BI GAGCTC 1 cut(s) 915
PspEI GGTNACC 1 cut(s) 272
PspN4I GGNNCC 4 cut(s) 674, 687, 891, 937
PspPI GGNCC 1 cut(s) 686
PstNI CAGNNNCTG 2 cut(s) 149, 430
PsuI RGATCY 3 cut(s) 672, 826, 1120
PvuII CAGCTG 1 cut(s) 731
RsaI GTAC 4 cut(s) 8, 506, 863, 950
RsaNI GTAC 4 cut(s) 7, 505, 862, 949
SacI GAGCTC 1 cut(s) 915
SaqAI TTAA 2 cut(s) 488, 786
SatI GCNGC 5 cut(s) 162, 345, 840, 1074, 1077
Sau3AI GATC 7 cut(s) 78, 446, 547, 662, 672, 826, 1120
Sau96I GGNCC 1 cut(s) 686
ScaI AGTACT 1 cut(s) 506
SchI GAGTC 4 cut(s) 128, 234, 274, 354
SduI GDGCHC 1 cut(s) 915
SfaNI GCATC 3 cut(s) 173, 472, 605
Sse9I AATT 9 cut(s) 139, 298, 313, 331, 389, 984, 1035, 1082, 1098
SsiI CCGC 2 cut(s) 162, 345
SspI AATATT 1 cut(s) 477
SspMI CTAG 1 cut(s) 158
SstI GAGCTC 1 cut(s) 915
StyI CCWWGG 2 cut(s) 87, 495
TaaI ACNGT 2 cut(s) 104, 991
TaqI TCGA 3 cut(s) 249, 466, 531
TasI AATT 9 cut(s) 139, 298, 313, 331, 389, 984, 1035, 1082, 1098
TatI WGTACW 3 cut(s) 504, 861, 948
TauI GCSGC 2 cut(s) 164, 347
TfiI GAWTC 2 cut(s) 225, 368
Tru1I TTAA 2 cut(s) 488, 786
Tru9I TTAA 2 cut(s) 488, 786
TscAI CASTG 3 cut(s) 346, 622, 970
TseFI GTSAC 2 cut(s) 272, 1148
TseI GCWGC 3 cut(s) 839, 1073, 1076
Tsp45I GTSAC 2 cut(s) 272, 1148
TspDTI ATGAA 3 cut(s) 783, 1008, 1049
TspRI CASTG 3 cut(s) 346, 622, 970
XapI RAATTY 7 cut(s) 139, 298, 313, 331, 389, 1082, 1098
XceI RCATGY 1 cut(s) 1149
XcmI CCANNNNNNNNNTGG 1 cut(s) 1010
XspI CTAG 1 cut(s) 158
ZrmI AGTACT 1 cut(s) 506
Zsp2I ATGCAT 1 cut(s) 598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.