pycom15g31220

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0000978
Physical Location & Seq
Forward (+)
29569469 .. 29573736
4268 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g31220.1

Sequence Viewer

Length: 1173 bp
ATGGAGTCCCCGATAATTACTATCAAAGTTATCATTTCATTCTTTTGCTCGTACATGCTTTTGACGCACTTGCATCTCACCACTTTGAACCCTACTGCCTTTGCTTTGGAGGACTGTCAGTTTCCAGCCGTCTTTAACCTTGGGGACTCTAATTCAGATACTGGCGGATTGGCCTCATCCCTTATTGCACCAACCCCTCCTTATGGCGAGACATTTTTTGGTATGCCGGAGGGAAGATTCTCAGATGGCCGTCTCATAATCGATTTCCTCGCAAATAGTGTTGGCCACCCCTTTCTAAGCGCATATTTGGATTCGCTTGGGACCAACTTCTCGTACGGTGCAAATTTTGCCACTGCAGCTTCCACAATCAGGCTGCCCAATAGCGTTATACCAAATGGTGGATTTAGCCCTTTCTTCCTTGATATTCAGTACATGCAGTTCATGCGACTCAAATTCAGATCACGACTTATAAGGCAAAGAGGAGGAATATTTGCAAGTTTACTGCCCAAGGATGAGTACTTCCCCAAAGCTTTATACACATTTGACATTGGTCAGAACGACCTTGGGGAAGGGTTCTTTGCAAACAAGACTATTAAGGAAGTGAATGCATCTGTTCCTGATATAATCACCAAGTTCTCAACAAATATTCAGAAAATATATGACTTGGGAGCCAGATCGTTTTGGATCCACAACACTGGGCCAATTGGCTGTCTCCCTTACATTTTAGCTAATTTTCCATCAGCTCAAAAGGATGAGGTTGGCTGCGCAAAGTCTTACAATGAAGTGGCTCAGTATTTTAACCACCAATTGAAGGGAGCCACAGTTCAACTTAGGAAGGATCTTCCTTTGGCTGCATTTACTTACGTGGACATATATTCTGTCAAATACTCTCTTTACAAGGAGCCCAAAAAATATGGGTTTGAGTTCCCACTTGTCGCTTGTTGTGGGTTTGGTGGGAAGTACAACTATAGCAGTAGTGCTGGATGTGGAACAACAGTTACAGTCAATGGAAGCCAAATATTTGTTGGTTCGTGCAAAAACCCGTCAGTGAGAGTGAATTGGGATGGCGTTCATTATACTGAGGCAGCTAACAAGTTTGTTTTTGACAAAATTTCAACTGGAGCATTTTCAGATCCACCCCTTCCTTTAAAACATGCATGTCACAGGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005488 GO:0006950 GO:0006952 GO:0008150 GO:0016787 GO:0016788 GO:0019863 GO:0019865 GO:0044877 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

391

Amino Acids

43.06

Weight (kDa)

8.26

Isoelectric Point (pI)

32.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 44 - 371 1.1e-44 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 470
Acc16I TGCGCA 1 cut(s) 766
AccB7I CCANNNNNTGG 1 cut(s) 398
AciI CCGC 1 cut(s) 165
AclWI GGATC 4 cut(s) 679, 692, 846, 1127
AcoI YGGCCR 2 cut(s) 247, 283
AcsI RAATTY 3 cut(s) 343, 452, 1110
AfaI GTAC 5 cut(s) 53, 335, 431, 518, 962
AfiI CCNNNNNNNGG 4 cut(s) 203, 369, 398, 811
AgsI TTSAA 4 cut(s) 88, 811, 827, 1116
AloI GAACNNNNNNTCC 2 cut(s) 807, 839
AluBI AGCT 5 cut(s) 359, 530, 728, 743, 1088
AluI AGCT 5 cut(s) 359, 530, 728, 743, 1088
Alw26I GTCTC 3 cut(s) 203, 257, 716
AlwI GGATC 4 cut(s) 679, 692, 846, 1127
AlwNI CAGNNNCTG 1 cut(s) 161
AoxI GGCC 4 cut(s) 171, 247, 283, 698
ApeKI GCWGC 5 cut(s) 356, 373, 762, 851, 1085
ApoI RAATTY 3 cut(s) 343, 452, 1110
AspLEI GCGC 2 cut(s) 302, 767
AspS9I GGNCC 2 cut(s) 321, 698
AsuHPI GGTGA 2 cut(s) 70, 619
AvaII GGWCC 1 cut(s) 321
BalI TGGCCA 1 cut(s) 285
BamHI GGATCC 1 cut(s) 684
BanII GRGCYC 1 cut(s) 906
BbvI GCAGC 5 cut(s) 360, 368, 749, 838, 1097
BccI CCATC 3 cut(s) 239, 745, 1058
BceAI ACGGC 2 cut(s) 113, 234
BcoDI GTCTC 3 cut(s) 203, 257, 716
BfmI CTRYAG 2 cut(s) 354, 967
BisI GCNGC 5 cut(s) 357, 374, 763, 852, 1086
BlsI GCNGC 5 cut(s) 358, 375, 764, 853, 1087
BmcAI AGTACT 1 cut(s) 518
Bme18I GGWCC 1 cut(s) 321
BmgT120I GGNCC 2 cut(s) 321, 698
BmiI GGNNCC 5 cut(s) 322, 670, 686, 817, 903
BmrI ACTGGG 1 cut(s) 705
BmsI GCATC 2 cut(s) 82, 617
BmuI ACTGGG 1 cut(s) 705
BoxI GACNNNNGTC 1 cut(s) 549
BpmI CTGGAG 1 cut(s) 1140
Bsa29I ATCGAT 1 cut(s) 261
BsaAI YACGTR 1 cut(s) 865
BsaJI CCNNGG 3 cut(s) 139, 507, 562
BsaXI ACNNNNNCTCC 2 cut(s) 807, 837
Bsc4I CCNNNNNNNGG 4 cut(s) 203, 369, 398, 811
Bse1I ACTGG 3 cut(s) 166, 700, 1123
BseCI ATCGAT 1 cut(s) 261
BseDI CCNNGG 3 cut(s) 139, 507, 562
BseGI GGATG 5 cut(s) 176, 517, 757, 989, 1069
BseLI CCNNNNNNNGG 4 cut(s) 203, 369, 398, 811
BseMII CTCAG 3 cut(s) 255, 803, 1071
BseNI ACTGG 3 cut(s) 166, 700, 1123
BseRI GAGGAG 1 cut(s) 495
BseXI GCAGC 5 cut(s) 360, 368, 749, 838, 1097
BshFI GGCC 4 cut(s) 173, 249, 285, 700
BshVI ATCGAT 1 cut(s) 261
BsiSI CCGG 1 cut(s) 227
BsiWI CGTACG 1 cut(s) 333
BslFI GGGAC 2 cut(s) 158, 334
BslI CCNNNNNNNGG 4 cut(s) 203, 369, 398, 811
BsmAI GTCTC 3 cut(s) 203, 257, 716
BsmBI CGTCTC 1 cut(s) 257
BsmFI GGGAC 2 cut(s) 158, 334
BsmI GAATGC 1 cut(s) 610
BsnI GGCC 4 cut(s) 173, 249, 285, 700
Bsp1286I GDGCHC 1 cut(s) 906
Bsp143I GATC 5 cut(s) 458, 674, 684, 838, 1132
BspACI CCGC 1 cut(s) 165
BspANI GGCC 4 cut(s) 173, 249, 285, 700
BspCNI CTCAG 3 cut(s) 254, 802, 1072
BspDI ATCGAT 1 cut(s) 261
BspLI GGNNCC 5 cut(s) 322, 670, 686, 817, 903
BspMAI CTGCAG 1 cut(s) 358
BspPI GGATC 4 cut(s) 679, 692, 846, 1127
BsrI ACTGG 3 cut(s) 166, 700, 1123
BssECI CCNNGG 3 cut(s) 139, 507, 562
BssMI GATC 5 cut(s) 458, 674, 684, 838, 1132
BssT1I CCWWGG 3 cut(s) 139, 507, 562
Bst4CI ACNGT 5 cut(s) 116, 338, 823, 997, 1003
BstAPI GCANNNNNTGC 2 cut(s) 347, 442
BstBAI YACGTR 1 cut(s) 865
BstDEI CTNAG 5 cut(s) 241, 296, 789, 830, 1080
BstF5I GGATG 5 cut(s) 176, 517, 757, 989, 1069
BstHHI GCGC 2 cut(s) 302, 767
BstKTI GATC 5 cut(s) 461, 677, 687, 841, 1135
BstMAI GTCTC 3 cut(s) 203, 257, 716
BstMBI GATC 5 cut(s) 458, 674, 684, 838, 1132
BstMWI GCNNNNNNNGC 4 cut(s) 64, 347, 356, 442
BstNSI RCATGY 4 cut(s) 58, 436, 1157, 1161
BstPAI GACNNNNGTC 1 cut(s) 549
BstSFI CTRYAG 2 cut(s) 354, 967
BstV1I GCAGC 5 cut(s) 360, 368, 749, 838, 1097
BstX2I RGATCY 3 cut(s) 684, 838, 1132
BstXI CCANNNNNNTGG 1 cut(s) 695
BstYI RGATCY 3 cut(s) 684, 838, 1132
Bsu15I ATCGAT 1 cut(s) 261
BsuRI GGCC 4 cut(s) 173, 249, 285, 700
BsuTUI ATCGAT 1 cut(s) 261
BtsCI GGATG 5 cut(s) 176, 517, 757, 989, 1069
BtsI GCAGTG 1 cut(s) 351
BtsIMutI CAGTG 3 cut(s) 351, 693, 1053
CaiI CAGNNNCTG 1 cut(s) 161
CfoI GCGC 2 cut(s) 302, 767
Cfr13I GGNCC 2 cut(s) 321, 698
ClaI ATCGAT 1 cut(s) 261
CseI GACGC 1 cut(s) 73
Csp6I GTAC 5 cut(s) 52, 334, 430, 517, 961
CviAII CATG 5 cut(s) 55, 433, 442, 1154, 1158
CviQI GTAC 5 cut(s) 52, 334, 430, 517, 961
DdeI CTNAG 5 cut(s) 241, 296, 789, 830, 1080
DpnI GATC 5 cut(s) 460, 676, 686, 840, 1134
DpnII GATC 5 cut(s) 458, 674, 684, 838, 1132
DraI TTTAAA 1 cut(s) 1149
EaeI YGGCCR 2 cut(s) 247, 283
EciI GGCGGA 1 cut(s) 180
Eco130I CCWWGG 3 cut(s) 139, 507, 562
Eco24I GRGCYC 1 cut(s) 906
Eco47I GGWCC 1 cut(s) 321
EcoT14I CCWWGG 3 cut(s) 139, 507, 562
EcoT22I ATGCAT 2 cut(s) 610, 1159
EcoT38I GRGCYC 1 cut(s) 906
ErhI CCWWGG 3 cut(s) 139, 507, 562
Esp3I CGTCTC 1 cut(s) 257
FaeI CATG 5 cut(s) 58, 436, 445, 1157, 1161
FaqI GGGAC 2 cut(s) 158, 334
FatI CATG 5 cut(s) 54, 432, 441, 1153, 1157
Fnu4HI GCNGC 5 cut(s) 357, 374, 763, 852, 1086
FokI GGATG 5 cut(s) 163, 524, 764, 996, 1076
FriOI GRGCYC 1 cut(s) 906
Fsp4HI GCNGC 5 cut(s) 357, 374, 763, 852, 1086
FspI TGCGCA 1 cut(s) 766
GlaI GCGC 2 cut(s) 301, 766
GluI GCNGC 5 cut(s) 357, 374, 763, 852, 1086
GsuI CTGGAG 1 cut(s) 1140
HaeIII GGCC 4 cut(s) 173, 249, 285, 700
HapII CCGG 1 cut(s) 227
HgaI GACGC 1 cut(s) 73
HhaI GCGC 2 cut(s) 302, 767
Hin1II CATG 5 cut(s) 58, 436, 445, 1157, 1161
Hin6I GCGC 2 cut(s) 300, 765
HinP1I GCGC 2 cut(s) 300, 765
HindIII AAGCTT 1 cut(s) 528
HinfI GANTC 5 cut(s) 5, 146, 237, 311, 447
HpaII CCGG 1 cut(s) 227
HphI GGTGA 2 cut(s) 70, 619
Hpy166II GTNNAC 2 cut(s) 500, 868
Hpy188I TCNGA 6 cut(s) 157, 244, 458, 555, 651, 1132
Hpy188III TCNNGA 2 cut(s) 462, 617
Hpy8I GTNNAC 2 cut(s) 500, 868
HpyAV CCTTC 4 cut(s) 563, 805, 829, 1151
HpyCH4III ACNGT 5 cut(s) 116, 338, 823, 997, 1003
HpyCH4IV ACGT 1 cut(s) 864
HpyF10VI GCNNNNNNNGC 4 cut(s) 64, 347, 356, 442
HpyF3I CTNAG 5 cut(s) 241, 296, 789, 830, 1080
HpySE526I ACGT 1 cut(s) 864
Hsp92II CATG 5 cut(s) 58, 436, 445, 1157, 1161
HspAI GCGC 2 cut(s) 300, 765
Kzo9I GATC 5 cut(s) 458, 674, 684, 838, 1132
LmnI GCTCC 4 cut(s) 668, 815, 901, 1121
Lsp1109I GCAGC 5 cut(s) 360, 368, 749, 838, 1097
LweI GCATC 2 cut(s) 82, 617
MaeII ACGT 1 cut(s) 864
MaeIII GTNAC 2 cut(s) 997, 1160
MalI GATC 5 cut(s) 460, 676, 686, 840, 1134
MboI GATC 5 cut(s) 458, 674, 684, 838, 1132
MboII GAAGA 3 cut(s) 246, 406, 833
MfeI CAATTG 2 cut(s) 702, 806
MflI RGATCY 3 cut(s) 684, 838, 1132
MhlI GDGCHC 1 cut(s) 906
MlsI TGGCCA 1 cut(s) 285
MluCI AATT 9 cut(s) 15, 151, 343, 452, 702, 730, 806, 1057, 1110
MluNI TGGCCA 1 cut(s) 285
MlyI GAGTC 3 cut(s) 14, 140, 441
MnlI CCTC 9 cut(s) 103, 184, 207, 223, 278, 473, 476, 748, 1075
Mox20I TGGCCA 1 cut(s) 285
Mph1103I ATGCAT 2 cut(s) 610, 1159
MscI TGGCCA 1 cut(s) 285
MseI TTAA 4 cut(s) 135, 594, 798, 1148
Msp20I TGGCCA 1 cut(s) 285
MspI CCGG 1 cut(s) 227
MunI CAATTG 2 cut(s) 702, 806
Mva1269I GAATGC 1 cut(s) 610
MwoI GCNNNNNNNGC 4 cut(s) 64, 347, 356, 442
NdeII GATC 5 cut(s) 458, 674, 684, 838, 1132
NlaIII CATG 5 cut(s) 58, 436, 445, 1157, 1161
NlaIV GGNNCC 5 cut(s) 322, 670, 686, 817, 903
NmuCI GTSAC 1 cut(s) 1160
NsbI TGCGCA 1 cut(s) 766
NsiI ATGCAT 2 cut(s) 610, 1159
NspI RCATGY 4 cut(s) 58, 436, 1157, 1161
PctI GAATGC 1 cut(s) 610
PfeI GAWTC 2 cut(s) 237, 311
Pfl23II CGTACG 1 cut(s) 333
PflMI CCANNNNNTGG 1 cut(s) 398
PkrI GCNGC 5 cut(s) 358, 375, 764, 853, 1087
PleI GAGTC 3 cut(s) 13, 140, 441
PpsI GAGTC 3 cut(s) 13, 140, 441
Ppu21I YACGTR 1 cut(s) 865
PshAI GACNNNNGTC 1 cut(s) 549
PsiI TTATAA 1 cut(s) 470
PspLI CGTACG 1 cut(s) 333
PspN4I GGNNCC 5 cut(s) 322, 670, 686, 817, 903
PspPI GGNCC 2 cut(s) 321, 698
PsrI GAACNNNNNNTAC 4 cut(s) 422, 454, 982, 1014
PstI CTGCAG 1 cut(s) 358
PstNI CAGNNNCTG 1 cut(s) 161
PsuI RGATCY 3 cut(s) 684, 838, 1132
RsaI GTAC 5 cut(s) 53, 335, 431, 518, 962
RsaNI GTAC 5 cut(s) 52, 334, 430, 517, 961
SaqAI TTAA 4 cut(s) 135, 594, 798, 1148
SatI GCNGC 5 cut(s) 357, 374, 763, 852, 1086
Sau3AI GATC 5 cut(s) 458, 674, 684, 838, 1132
Sau96I GGNCC 2 cut(s) 321, 698
ScaI AGTACT 1 cut(s) 518
SchI GAGTC 3 cut(s) 14, 140, 441
SduI GDGCHC 1 cut(s) 906
SetI ASST 9 cut(s) 141, 361, 532, 564, 730, 745, 759, 867, 1090
SfaNI GCATC 2 cut(s) 82, 617
SfcI CTRYAG 2 cut(s) 354, 967
SinI GGWCC 1 cut(s) 321
Sse9I AATT 9 cut(s) 15, 151, 343, 452, 702, 730, 806, 1057, 1110
SsiI CCGC 1 cut(s) 165
SspI AATATT 3 cut(s) 489, 646, 1020
StyI CCWWGG 3 cut(s) 139, 507, 562
TaaI ACNGT 5 cut(s) 116, 338, 823, 997, 1003
TaiI ACGT 1 cut(s) 867
TaqI TCGA 1 cut(s) 261
TasI AATT 9 cut(s) 15, 151, 343, 452, 702, 730, 806, 1057, 1110
TatI WGTACW 3 cut(s) 429, 516, 960
TfiI GAWTC 2 cut(s) 237, 311
Tru1I TTAA 4 cut(s) 135, 594, 798, 1148
Tru9I TTAA 4 cut(s) 135, 594, 798, 1148
TscAI CASTG 3 cut(s) 358, 700, 1053
TseFI GTSAC 1 cut(s) 1160
TseI GCWGC 5 cut(s) 356, 373, 762, 851, 1085
Tsp45I GTSAC 1 cut(s) 1160
TspDTI ATGAA 4 cut(s) 27, 430, 795, 1061
TspRI CASTG 3 cut(s) 358, 700, 1053
Van91I CCANNNNNTGG 1 cut(s) 398
VpaK11BI GGWCC 1 cut(s) 321
XapI RAATTY 3 cut(s) 343, 452, 1110
XceI RCATGY 4 cut(s) 58, 436, 1157, 1161
XcmI CCANNNNNNNNNTGG 1 cut(s) 1022
ZrmI AGTACT 1 cut(s) 518
Zsp2I ATGCAT 2 cut(s) 610, 1159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.