Prupe.1G497700_v2.0.a1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000978
Physical Location & Seq
Forward (+)
41233257 .. 41236424
3168 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G497700.1

Sequence Viewer

Length: 1167 bp
ATGGAGTCTCCTAGAATTCCTATCAAAGCTATCGTTTCTCTATTTTGCTCTTATATGCTTTTGTGGCATATCACCACTTTGAGCCCTACCTTGGCTTTGGAAGACTGTGAGTTTCCAGCCATCTTTAACTTTGGAGATTCAAATTCAGATACTGGTGGATTGGCTGCATCCCTTTCGGCACCACCACCACCTTATGGGGAGACCTATTTTCATATGCCGGTTGGAAGATTCTCAGATGGCAGGGTCATAATCGATTTTCTTGCAAAGAATCTTGGTCACTCCTTTCTAAGCGCATATCTGGATTCCCTTGGGAGCAACTTCTCGTATGGTGCAAATTTTGCCACTGGAGGCTCCACCATCAGATTGCCAGATCAAATTTTTCCAGCTGGTGGATTTAGTCCCTTCTACCTCAATATTCAGTACATGCAGTTCATGCAACTAAAATCCAGATCACAACTCATTAGGCATCGAGGGGGAATATTTGCAAGTTTGATGCCCAAGGAGTCGTATTTCTCGAAAGCTTTATACACATTTGACATTGGCCAGAATGACCTAACAGAAGGGTTCATTGGTAACATGACTGTTCAACAAGTCAATGCATCTGTTCCTGATATAATCATTGGCCTCTCGGCAAATATTAAGAAAATATATGATTTGGGAGCGAGATCATTTTGGATCCACAACACTGGGCCAATTGGCTGTCTCCCTTCCATCTTAGCCAATTTACCAGGTCAAAAGGATGAGGCTGGCTGTGCAAAGTCATATAATGAAGTAGCTCAGCATTTTAACCAGAAGCTGAAGGAAGCCACAGTTCAACTTAGGAAAGATCTACCTTTGGCTGCAATTACTTATGTGGACGTCTATTCAGTTAAATACTCTCTTTTCAAGGAACCGCAAAAATATGGGTTTGAGCTCCCACTTGTTGCTTGTTGTGGGTATGGTGGCAAGTACAACTATAACAGTAGTTCTCAATGTGGAGGAACAGCTAGAGTTAATGGAAGGCAAACATTTGTTGGCTCATGCAAAGATCCTTCGGTGAGAGTGAATTGGGATGGCACTCATTATACTGAGGCAGCTGCCAAGTTTATTTCTGATAAAATTTCAACTGGAGCATTTTCTGATCCACCCCTTGCTTTGAAACAAGCATGTCACAAGAGTTTGGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005488 GO:0006950 GO:0006952 GO:0008150 GO:0016787 GO:0016788 GO:0019863 GO:0019865 GO:0044877 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

389

Amino Acids

42.46

Weight (kDa)

8.26

Isoelectric Point (pI)

42.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 861
AccB1I GGYRCC 1 cut(s) 178
AccB7I CCANNNNNTGG 2 cut(s) 194, 389
AciI CCGC 1 cut(s) 893
AclWI GGATC 4 cut(s) 670, 683, 1022, 1115
AcoI YGGCCR 1 cut(s) 541
AcsI RAATTY 5 cut(s) 15, 142, 334, 375, 1098
AcuI CTGAAG 1 cut(s) 818
AcyI GRCGYC 1 cut(s) 858
AfaI GTAC 2 cut(s) 422, 950
AfiI CCNNNNNNNGG 3 cut(s) 91, 194, 389
AgsI TTSAA 6 cut(s) 141, 587, 815, 886, 1104, 1138
AjnI CCWGG 1 cut(s) 727
AjuI GAANNNNNNNTTGG 2 cut(s) 552, 584
AluBI AGCT 8 cut(s) 29, 386, 521, 776, 796, 913, 986, 1076
AluI AGCT 8 cut(s) 29, 386, 521, 776, 796, 913, 986, 1076
Alw21I GWGCWC 1 cut(s) 915
Alw26I GTCTC 3 cut(s) 12, 194, 707
AlwI GGATC 4 cut(s) 670, 683, 1022, 1115
AlwNI CAGNNNCTG 2 cut(s) 152, 796
AoxI GGCC 4 cut(s) 541, 622, 689, 1161
ApeKI GCWGC 4 cut(s) 164, 839, 1073, 1076
ApoI RAATTY 5 cut(s) 15, 142, 334, 375, 1098
AspLEI GCGC 1 cut(s) 293
AspS9I GGNCC 1 cut(s) 689
AsuHPI GGTGA 2 cut(s) 64, 1048
BalI TGGCCA 1 cut(s) 543
BamHI GGATCC 1 cut(s) 675
BanI GGYRCC 1 cut(s) 178
BanII GRGCYC 2 cut(s) 86, 915
BbsI GAAGAC 1 cut(s) 108
Bbv12I GWGCWC 1 cut(s) 915
BbvI GCAGC 4 cut(s) 151, 826, 1063, 1085
BccI CCATC 5 cut(s) 128, 230, 365, 719, 1046
BcgI CGANNNNNNTGC 4 cut(s) 156, 190, 242, 276
BciT130I CCWGG 1 cut(s) 729
BcoDI GTCTC 3 cut(s) 12, 194, 707
BfaI CTAG 2 cut(s) 12, 987
BglII AGATCT 1 cut(s) 826
BisI GCNGC 4 cut(s) 165, 840, 1074, 1077
BlpI GCTNAGC 1 cut(s) 777
BlsI GCNGC 4 cut(s) 166, 841, 1075, 1078
Bme1390I CCNGG 1 cut(s) 729
BmgT120I GGNCC 1 cut(s) 689
BmiI GGNNCC 4 cut(s) 180, 352, 677, 891
BmrFI CCNGG 1 cut(s) 729
BmrI ACTGGG 1 cut(s) 696
BmsI GCATC 4 cut(s) 176, 475, 483, 608
BmuI ACTGGG 1 cut(s) 696
BpiI GAAGAC 1 cut(s) 108
BpmI CTGGAG 2 cut(s) 366, 1128
Bpu1102I GCTNAGC 1 cut(s) 777
Bsa29I ATCGAT 1 cut(s) 252
BsaHI GRCGYC 1 cut(s) 858
BsaI GGTCTC 1 cut(s) 194
BsaJI CCNNGG 3 cut(s) 90, 307, 498
Bsc4I CCNNNNNNNGG 3 cut(s) 91, 194, 389
Bse118I RCCGGY 1 cut(s) 217
Bse1I ACTGG 4 cut(s) 157, 349, 691, 1111
BseBI CCWGG 1 cut(s) 729
BseCI ATCGAT 1 cut(s) 252
BseDI CCNNGG 3 cut(s) 90, 307, 498
BseGI GGATG 3 cut(s) 167, 745, 1057
BseLI CCNNNNNNNGG 3 cut(s) 91, 194, 389
BseMII CTCAG 3 cut(s) 246, 791, 1059
BseNI ACTGG 4 cut(s) 157, 349, 691, 1111
BseXI GCAGC 4 cut(s) 151, 826, 1063, 1085
BshFI GGCC 4 cut(s) 543, 624, 691, 1163
BshNI GGYRCC 1 cut(s) 178
BshVI ATCGAT 1 cut(s) 252
BsiHKAI GWGCWC 1 cut(s) 915
BsiSI CCGG 1 cut(s) 218
BslFI GGGAC 1 cut(s) 384
BslI CCNNNNNNNGG 3 cut(s) 91, 194, 389
BsmAI GTCTC 3 cut(s) 12, 194, 707
BsmFI GGGAC 1 cut(s) 384
BsnI GGCC 4 cut(s) 543, 624, 691, 1163
Bso31I GGTCTC 1 cut(s) 194
Bsp1286I GDGCHC 2 cut(s) 86, 915
Bsp143I GATC 7 cut(s) 370, 449, 665, 675, 826, 1027, 1120
Bsp1720I GCTNAGC 1 cut(s) 777
BspACI CCGC 1 cut(s) 893
BspANI GGCC 4 cut(s) 543, 624, 691, 1163
BspCNI CTCAG 3 cut(s) 245, 790, 1060
BspDI ATCGAT 1 cut(s) 252
BspLI GGNNCC 4 cut(s) 180, 352, 677, 891
BspPI GGATC 4 cut(s) 670, 683, 1022, 1115
BspT107I GGYRCC 1 cut(s) 178
BspTNI GGTCTC 1 cut(s) 194
BsrFI RCCGGY 1 cut(s) 217
BsrI ACTGG 4 cut(s) 157, 349, 691, 1111
BssAI RCCGGY 1 cut(s) 217
BssECI CCNNGG 3 cut(s) 90, 307, 498
BssMI GATC 7 cut(s) 370, 449, 665, 675, 826, 1027, 1120
BssNI GRCGYC 1 cut(s) 858
BssT1I CCWWGG 3 cut(s) 90, 307, 498
Bst2UI CCWGG 1 cut(s) 729
Bst4CI ACNGT 4 cut(s) 107, 583, 811, 962
BstACI GRCGYC 1 cut(s) 858
BstAPI GCANNNNNTGC 2 cut(s) 338, 433
BstC8I GCNNGC 1 cut(s) 748
BstDEI CTNAG 6 cut(s) 232, 287, 715, 777, 818, 1068
BstF5I GGATG 3 cut(s) 167, 745, 1057
BstHHI GCGC 1 cut(s) 293
BstKTI GATC 7 cut(s) 373, 452, 668, 678, 829, 1030, 1123
BstMAI GTCTC 3 cut(s) 12, 194, 707
BstMBI GATC 7 cut(s) 370, 449, 665, 675, 826, 1027, 1120
BstMWI GCNNNNNNNGC 4 cut(s) 64, 338, 433, 752
BstNI CCWGG 1 cut(s) 729
BstNSI RCATGY 2 cut(s) 427, 1149
BstSCI CCNGG 1 cut(s) 727
BstV1I GCAGC 4 cut(s) 151, 826, 1063, 1085
BstV2I GAAGAC 1 cut(s) 108
BstX2I RGATCY 3 cut(s) 675, 826, 1027
BstXI CCANNNNNNTGG 1 cut(s) 686
BstYI RGATCY 3 cut(s) 675, 826, 1027
Bsu15I ATCGAT 1 cut(s) 252
BsuRI GGCC 4 cut(s) 543, 624, 691, 1163
BsuTUI ATCGAT 1 cut(s) 252
BtsCI GGATG 3 cut(s) 167, 745, 1057
BtsIMutI CAGTG 2 cut(s) 342, 684
Cac8I GCNNGC 1 cut(s) 748
CaiI CAGNNNCTG 2 cut(s) 152, 796
CfoI GCGC 1 cut(s) 293
Cfr10I RCCGGY 1 cut(s) 217
Cfr13I GGNCC 1 cut(s) 689
ClaI ATCGAT 1 cut(s) 252
CsiI ACCWGGT 1 cut(s) 727
Csp6I GTAC 2 cut(s) 421, 949
CviAII CATG 5 cut(s) 424, 433, 577, 1020, 1146
CviQI GTAC 2 cut(s) 421, 949
DdeI CTNAG 6 cut(s) 232, 287, 715, 777, 818, 1068
DpnI GATC 7 cut(s) 372, 451, 667, 677, 828, 1029, 1122
DpnII GATC 7 cut(s) 370, 449, 665, 675, 826, 1027, 1120
EaeI YGGCCR 1 cut(s) 541
Ecl136II GAGCTC 1 cut(s) 913
Eco130I CCWWGG 3 cut(s) 90, 307, 498
Eco24I GRGCYC 2 cut(s) 86, 915
Eco31I GGTCTC 1 cut(s) 194
Eco53kI GAGCTC 1 cut(s) 913
Eco57I CTGAAG 1 cut(s) 818
EcoICRI GAGCTC 1 cut(s) 913
EcoRI GAATTC 1 cut(s) 15
EcoRII CCWGG 1 cut(s) 727
EcoT14I CCWWGG 3 cut(s) 90, 307, 498
EcoT22I ATGCAT 1 cut(s) 601
EcoT38I GRGCYC 2 cut(s) 86, 915
ErhI CCWWGG 3 cut(s) 90, 307, 498
FaeI CATG 5 cut(s) 427, 436, 580, 1023, 1149
FaqI GGGAC 1 cut(s) 384
FatI CATG 5 cut(s) 423, 432, 576, 1019, 1145
FauNDI CATATG 1 cut(s) 213
Fnu4HI GCNGC 4 cut(s) 165, 840, 1074, 1077
FokI GGATG 3 cut(s) 154, 752, 1064
FriOI GRGCYC 2 cut(s) 86, 915
Fsp4HI GCNGC 4 cut(s) 165, 840, 1074, 1077
FspBI CTAG 2 cut(s) 12, 987
GlaI GCGC 1 cut(s) 292
GluI GCNGC 4 cut(s) 165, 840, 1074, 1077
GsuI CTGGAG 2 cut(s) 366, 1128
HaeIII GGCC 4 cut(s) 543, 624, 691, 1163
HapII CCGG 1 cut(s) 218
HhaI GCGC 1 cut(s) 293
Hin1I GRCGYC 1 cut(s) 858
Hin1II CATG 5 cut(s) 427, 436, 580, 1023, 1149
Hin6I GCGC 1 cut(s) 291
HinP1I GCGC 1 cut(s) 291
HindIII AAGCTT 1 cut(s) 519
HinfI GANTC 6 cut(s) 5, 137, 228, 268, 302, 503
HpaII CCGG 1 cut(s) 218
HphI GGTGA 2 cut(s) 64, 1048
Hpy166II GTNNAC 1 cut(s) 856
Hpy188I TCNGA 5 cut(s) 148, 235, 362, 1093, 1120
Hpy188III TCNNGA 4 cut(s) 299, 447, 514, 608
Hpy8I GTNNAC 1 cut(s) 856
HpyAV CCTTC 6 cut(s) 412, 554, 717, 793, 993, 1041
HpyCH4III ACNGT 4 cut(s) 107, 583, 811, 962
HpyCH4IV ACGT 1 cut(s) 858
HpyF10VI GCNNNNNNNGC 4 cut(s) 64, 338, 433, 752
HpyF3I CTNAG 6 cut(s) 232, 287, 715, 777, 818, 1068
HpySE526I ACGT 1 cut(s) 858
Hsp92I GRCGYC 1 cut(s) 858
Hsp92II CATG 5 cut(s) 427, 436, 580, 1023, 1149
HspAI GCGC 1 cut(s) 291
Kzo9I GATC 7 cut(s) 370, 449, 665, 675, 826, 1027, 1120
LmnI GCTCC 5 cut(s) 312, 356, 659, 918, 1109
Lsp1109I GCAGC 4 cut(s) 151, 826, 1063, 1085
LweI GCATC 4 cut(s) 176, 475, 483, 608
MabI ACCWGGT 1 cut(s) 727
MaeI CTAG 2 cut(s) 12, 987
MaeII ACGT 1 cut(s) 858
MaeIII GTNAC 3 cut(s) 275, 572, 1148
MalI GATC 7 cut(s) 372, 451, 667, 677, 828, 1029, 1122
MboI GATC 7 cut(s) 370, 449, 665, 675, 826, 1027, 1120
MboII GAAGA 2 cut(s) 113, 237
MfeI CAATTG 1 cut(s) 693
MflI RGATCY 3 cut(s) 675, 826, 1027
MhlI GDGCHC 2 cut(s) 86, 915
MlsI TGGCCA 1 cut(s) 543
MluCI AATT 9 cut(s) 15, 142, 334, 375, 693, 721, 843, 1045, 1098
MluNI TGGCCA 1 cut(s) 543
MlyI GAGTC 2 cut(s) 14, 512
MmeI TCCRAC 1 cut(s) 202
MnlI CCTC 7 cut(s) 341, 419, 464, 635, 736, 971, 1063
Mox20I TGGCCA 1 cut(s) 543
Mph1103I ATGCAT 1 cut(s) 601
MscI TGGCCA 1 cut(s) 543
MseI TTAA 5 cut(s) 126, 639, 786, 870, 993
Msp20I TGGCCA 1 cut(s) 543
MspA1I CMGCKG 2 cut(s) 386, 1076
MspI CCGG 1 cut(s) 218
MspR9I CCNGG 1 cut(s) 729
MunI CAATTG 1 cut(s) 693
MvaI CCWGG 1 cut(s) 729
MwoI GCNNNNNNNGC 4 cut(s) 64, 338, 433, 752
NdeI CATATG 1 cut(s) 213
NdeII GATC 7 cut(s) 370, 449, 665, 675, 826, 1027, 1120
NlaIII CATG 5 cut(s) 427, 436, 580, 1023, 1149
NlaIV GGNNCC 4 cut(s) 180, 352, 677, 891
NmeAIII GCCGAG 1 cut(s) 608
NmuCI GTSAC 2 cut(s) 275, 1148
NsiI ATGCAT 1 cut(s) 601
NspI RCATGY 2 cut(s) 427, 1149
PcsI WCGNNNNNNNCGW 1 cut(s) 512
PfeI GAWTC 4 cut(s) 137, 228, 268, 302
PflMI CCANNNNNTGG 2 cut(s) 194, 389
PkrI GCNGC 4 cut(s) 166, 841, 1075, 1078
PleI GAGTC 2 cut(s) 13, 511
PpsI GAGTC 2 cut(s) 13, 511
Psp124BI GAGCTC 1 cut(s) 915
Psp6I CCWGG 1 cut(s) 727
PspGI CCWGG 1 cut(s) 727
PspN4I GGNNCC 4 cut(s) 180, 352, 677, 891
PspPI GGNCC 1 cut(s) 689
PsrI GAACNNNNNNTAC 2 cut(s) 413, 445
PstNI CAGNNNCTG 2 cut(s) 152, 796
PsuI RGATCY 3 cut(s) 675, 826, 1027
PvuII CAGCTG 2 cut(s) 386, 1076
RsaI GTAC 2 cut(s) 422, 950
RsaNI GTAC 2 cut(s) 421, 949
SacI GAGCTC 1 cut(s) 915
SaqAI TTAA 5 cut(s) 126, 639, 786, 870, 993
SatI GCNGC 4 cut(s) 165, 840, 1074, 1077
Sau3AI GATC 7 cut(s) 370, 449, 665, 675, 826, 1027, 1120
Sau96I GGNCC 1 cut(s) 689
SchI GAGTC 2 cut(s) 14, 512
ScrFI CCNGG 1 cut(s) 729
SduI GDGCHC 2 cut(s) 86, 915
SexAI ACCWGGT 1 cut(s) 727
SfaNI GCATC 4 cut(s) 176, 475, 483, 608
Sse9I AATT 9 cut(s) 15, 142, 334, 375, 693, 721, 843, 1045, 1098
SsiI CCGC 1 cut(s) 893
SspI AATATT 3 cut(s) 415, 480, 637
SspMI CTAG 2 cut(s) 12, 987
SstI GAGCTC 1 cut(s) 915
StyD4I CCNGG 1 cut(s) 727
StyI CCWWGG 3 cut(s) 90, 307, 498
TaaI ACNGT 4 cut(s) 107, 583, 811, 962
TaiI ACGT 1 cut(s) 861
TaqI TCGA 3 cut(s) 252, 469, 515
TasI AATT 9 cut(s) 15, 142, 334, 375, 693, 721, 843, 1045, 1098
TatI WGTACW 2 cut(s) 420, 948
TfiI GAWTC 4 cut(s) 137, 228, 268, 302
Tru1I TTAA 5 cut(s) 126, 639, 786, 870, 993
Tru9I TTAA 5 cut(s) 126, 639, 786, 870, 993
TscAI CASTG 2 cut(s) 349, 691
TseFI GTSAC 2 cut(s) 275, 1148
TseI GCWGC 4 cut(s) 164, 839, 1073, 1076
Tsp45I GTSAC 2 cut(s) 275, 1148
TspDTI ATGAA 4 cut(s) 200, 421, 556, 783
TspRI CASTG 2 cut(s) 349, 691
Van91I CCANNNNNTGG 2 cut(s) 194, 389
XapI RAATTY 5 cut(s) 15, 142, 334, 375, 1098
XceI RCATGY 2 cut(s) 427, 1149
XspI CTAG 2 cut(s) 12, 987
ZraI GACGTC 1 cut(s) 859
Zsp2I ATGCAT 1 cut(s) 601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.