FvH4_2g26900

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000978
Physical Location & Seq
Reverse (-)
21515175 .. 21518802
3628 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g26900.t1

Sequence Viewer

Length: 1176 bp
ATGGAGTACTCTTGTAACGTCATCATTTCTCTGTTCTGCTTCTATATGCTTATATTGCTTGCTACCAGTAGTAGTGCTGTGATCCCTGCCTTGGCTCTGAAAGACTGCGAGTTTCCAGCCATCTTCAACTTTGGAGACTCAAATTCAGATACTGGTGGACTGTCTGCTGCATCTCTTCTACCACTAACGACACCTTTTGGAGAGAGTTATTTCCGTATGCCATCCGGAAGATTCTCTGATGGAAGACTCATTATCGATTTCCTTGCAAAGAGTCTTGGTCACCCTTATCTAAGCGCATATCTGGATTCTGTTGGCACAAATTTCTCGCACGGTGCTAATTTCGCCACTGCTGCTTCCACTATCAGACTCCCAGATAAAGTTATGCCGCCAGTGGGTGGATATAGTCCTTTCTATCTTGATATTCAATATATGCAGTTCCTGCAATTAAAGTCCAGATCACAACTCATAAGGCAGCGAGGGGGAATATTTGCAAGTTTAATGCCCAAGGAGAAGTACTTCTCCAAAGCTTTATACACATTCGACATTGGCCAGAATGATCTCAACAAGGAAATTTTTGGTGACATGAATATAGAGGAAGTAAATGCAGCTATTCCTGATATAATCACTGGTTTCTTAACTAATATTAAGAAAATATATGATTTGGGAGCAAGATCATTATGGATCCATAATACAGGGCCGATTGGCTATCTGACTTACATTGTGGCAAACTTCCCATCAGCTGAGAAAGATGACGTCGGCTGTCTAAAGGCTTACAATGAAGTAGCTCAATATTTTAACCAGGAGCTGAAGCAGGCCGTAGTTCAACTCAGGAAGGATCTTCCTTTGGCTGCATTTACTTATGTAATCGTGTATTCTGTCAAGTACTCTCTTTACAAGGAACCTGAAAAGTATGGATTTGAGCTCCCACTGGTTGCTTGCTGTGGCTATGGTGGCAAGTACAACTATAACAGTAGTGCAGGATGTGGAACAACAATCACAGTCAATGGAAGCCAAATCTTTGTGGGTTCATGCAAAAACCCTTCAACTAGAGTGAACTGGGATGGTGCTCATTACACCGAGGCAGCTGCCAAGTTTATTTTTGATAAAGTTTCAACTGGAGCATACTCAGATCCACCTTTGCCGTTGAAACAAGCATGTCACAGGAGTTTGATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005488 GO:0006950 GO:0006952 GO:0008150 GO:0016787 GO:0016788 GO:0019863 GO:0019865 GO:0044877 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

392

Amino Acids

43.14

Weight (kDa)

6.93

Isoelectric Point (pI)

41.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 41 - 370 6.2e-44 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 756
AccB7I CCANNNNNTGG 1 cut(s) 395
AccIII TCCGGA 1 cut(s) 224
AciI CCGC 1 cut(s) 386
AclWI GGATC 5 cut(s) 76, 676, 689, 843, 1124
AcoI YGGCCR 1 cut(s) 547
AcsI RAATTY 3 cut(s) 142, 319, 570
AcuI CTGAAG 1 cut(s) 827
AcyI GRCGYC 1 cut(s) 753
AfaI GTAC 4 cut(s) 8, 515, 884, 959
AfiI CCNNNNNNNGG 3 cut(s) 91, 392, 395
AgsI TTSAA 6 cut(s) 127, 425, 824, 1044, 1113, 1147
AjnI CCWGG 1 cut(s) 798
AluBI AGCT 7 cut(s) 527, 608, 740, 785, 805, 922, 1085
AluI AGCT 7 cut(s) 527, 608, 740, 785, 805, 922, 1085
Alw21I GWGCWC 2 cut(s) 924, 1069
Alw26I GTCTC 1 cut(s) 129
AlwI GGATC 5 cut(s) 76, 676, 689, 843, 1124
AlwNI CAGNNNCTG 3 cut(s) 152, 439, 805
Aor13HI TCCGGA 1 cut(s) 224
AoxI GGCC 3 cut(s) 547, 695, 813
ApeKI GCWGC 7 cut(s) 167, 350, 472, 605, 848, 1082, 1085
ApoI RAATTY 3 cut(s) 142, 319, 570
Asp700I GAANNNNTTC 1 cut(s) 515
AspLEI GCGC 1 cut(s) 296
AspS9I GGNCC 1 cut(s) 695
AsuHPI GGTGA 2 cut(s) 272, 590
BalI TGGCCA 1 cut(s) 549
BamHI GGATCC 1 cut(s) 681
BanII GRGCYC 1 cut(s) 924
BbsI GAAGAC 1 cut(s) 250
Bbv12I GWGCWC 2 cut(s) 924, 1069
BbvI GCAGC 7 cut(s) 154, 337, 484, 617, 835, 1072, 1094
BccI CCATC 5 cut(s) 128, 229, 233, 742, 1055
BceAI ACGGC 2 cut(s) 800, 1126
BcgI CGANNNNNNTGC 4 cut(s) 245, 279, 1067, 1101
BciT130I CCWGG 1 cut(s) 800
BcoDI GTCTC 1 cut(s) 129
BfaI CTAG 1 cut(s) 1047
BisI GCNGC 8 cut(s) 168, 351, 386, 473, 606, 849, 1083, 1086
BlsI GCNGC 8 cut(s) 169, 352, 387, 474, 607, 850, 1084, 1087
BmcAI AGTACT 3 cut(s) 8, 515, 884
Bme1390I CCNGG 1 cut(s) 800
BmgT120I GGNCC 1 cut(s) 695
BmiI GGNNCC 2 cut(s) 683, 900
BmrFI CCNGG 1 cut(s) 800
BmrI ACTGGG 1 cut(s) 1066
BmsI GCATC 1 cut(s) 179
BmuI ACTGGG 1 cut(s) 1066
BpiI GAAGAC 1 cut(s) 250
BpmI CTGGAG 1 cut(s) 1137
Bsa29I ATCGAT 1 cut(s) 255
BsaHI GRCGYC 1 cut(s) 753
BsaJI CCNNGG 3 cut(s) 90, 504, 1077
BsaWI WCCGGW 1 cut(s) 224
Bsc4I CCNNNNNNNGG 3 cut(s) 91, 392, 395
Bse1I ACTGG 7 cut(s) 66, 157, 389, 631, 933, 1061, 1120
BseAI TCCGGA 1 cut(s) 224
BseBI CCWGG 1 cut(s) 800
BseCI ATCGAT 1 cut(s) 255
BseDI CCNNGG 3 cut(s) 90, 504, 1077
BseGI GGATG 3 cut(s) 221, 986, 1066
BseLI CCNNNNNNNGG 3 cut(s) 91, 392, 395
BseMII CTCAG 3 cut(s) 732, 841, 1140
BseNI ACTGG 7 cut(s) 66, 157, 389, 631, 933, 1061, 1120
BseXI GCAGC 7 cut(s) 154, 337, 484, 617, 835, 1072, 1094
BsgI GTGCAG 1 cut(s) 996
BshFI GGCC 3 cut(s) 549, 697, 815
BshVI ATCGAT 1 cut(s) 255
BsiHKAI GWGCWC 2 cut(s) 924, 1069
BsiSI CCGG 1 cut(s) 225
BslI CCNNNNNNNGG 3 cut(s) 91, 392, 395
BsmAI GTCTC 1 cut(s) 129
BsnI GGCC 3 cut(s) 549, 697, 815
Bsp1286I GDGCHC 2 cut(s) 924, 1069
Bsp13I TCCGGA 1 cut(s) 224
Bsp143I GATC 8 cut(s) 81, 455, 556, 671, 681, 835, 1129, 1170
BspACI CCGC 1 cut(s) 386
BspANI GGCC 3 cut(s) 549, 697, 815
BspCNI CTCAG 3 cut(s) 733, 840, 1139
BspDI ATCGAT 1 cut(s) 255
BspEI TCCGGA 1 cut(s) 224
BspLI GGNNCC 2 cut(s) 683, 900
BspPI GGATC 5 cut(s) 76, 676, 689, 843, 1124
BsrI ACTGG 7 cut(s) 66, 157, 389, 631, 933, 1061, 1120
BssECI CCNNGG 3 cut(s) 90, 504, 1077
BssMI GATC 8 cut(s) 81, 455, 556, 671, 681, 835, 1129, 1170
BssNI GRCGYC 1 cut(s) 753
BssT1I CCWWGG 2 cut(s) 90, 504
Bst2UI CCWGG 1 cut(s) 800
Bst4CI ACNGT 4 cut(s) 162, 332, 971, 1000
Bst6I CTCTTC 1 cut(s) 180
BstACI GRCGYC 1 cut(s) 753
BstAPI GCANNNNNTGC 1 cut(s) 439
BstC8I GCNNGC 3 cut(s) 60, 813, 937
BstDEI CTNAG 4 cut(s) 290, 741, 827, 1126
BstEII GGTNACC 1 cut(s) 278
BstF5I GGATG 3 cut(s) 221, 986, 1066
BstHHI GCGC 1 cut(s) 296
BstKTI GATC 8 cut(s) 84, 458, 559, 674, 684, 838, 1132, 1173
BstMAI GTCTC 1 cut(s) 129
BstMBI GATC 8 cut(s) 81, 455, 556, 671, 681, 835, 1129, 1170
BstMWI GCNNNNNNNGC 5 cut(s) 55, 341, 350, 439, 951
BstNI CCWGG 1 cut(s) 800
BstNSI RCATGY 1 cut(s) 1158
BstPI GGTNACC 1 cut(s) 278
BstSCI CCNGG 1 cut(s) 798
BstV1I GCAGC 7 cut(s) 154, 337, 484, 617, 835, 1072, 1094
BstV2I GAAGAC 1 cut(s) 250
BstX2I RGATCY 3 cut(s) 681, 835, 1129
BstYI RGATCY 3 cut(s) 681, 835, 1129
Bsu15I ATCGAT 1 cut(s) 255
BsuRI GGCC 3 cut(s) 549, 697, 815
BsuTUI ATCGAT 1 cut(s) 255
BtsCI GGATG 3 cut(s) 221, 986, 1066
BtsI GCAGTG 1 cut(s) 345
BtsIMutI CAGTG 4 cut(s) 345, 396, 624, 926
Cac8I GCNNGC 3 cut(s) 60, 813, 937
CaiI CAGNNNCTG 3 cut(s) 152, 439, 805
CfoI GCGC 1 cut(s) 296
Cfr13I GGNCC 1 cut(s) 695
ClaI ATCGAT 1 cut(s) 255
Csp6I GTAC 4 cut(s) 7, 514, 883, 958
CviAII CATG 3 cut(s) 583, 1029, 1155
CviQI GTAC 4 cut(s) 7, 514, 883, 958
DdeI CTNAG 4 cut(s) 290, 741, 827, 1126
DpnI GATC 8 cut(s) 83, 457, 558, 673, 683, 837, 1131, 1172
DpnII GATC 8 cut(s) 81, 455, 556, 671, 681, 835, 1129, 1170
EaeI YGGCCR 1 cut(s) 547
Eam1104I CTCTTC 1 cut(s) 180
EarI CTCTTC 1 cut(s) 180
Ecl136II GAGCTC 1 cut(s) 922
Eco130I CCWWGG 2 cut(s) 90, 504
Eco24I GRGCYC 1 cut(s) 924
Eco53kI GAGCTC 1 cut(s) 922
Eco57I CTGAAG 1 cut(s) 827
Eco91I GGTNACC 1 cut(s) 278
EcoICRI GAGCTC 1 cut(s) 922
EcoO65I GGTNACC 1 cut(s) 278
EcoRII CCWGG 1 cut(s) 798
EcoT14I CCWWGG 2 cut(s) 90, 504
EcoT38I GRGCYC 1 cut(s) 924
ErhI CCWWGG 2 cut(s) 90, 504
FaeI CATG 3 cut(s) 586, 1032, 1158
FatI CATG 3 cut(s) 582, 1028, 1154
Fnu4HI GCNGC 8 cut(s) 168, 351, 386, 473, 606, 849, 1083, 1086
FokI GGATG 3 cut(s) 208, 993, 1073
FriOI GRGCYC 1 cut(s) 924
Fsp4HI GCNGC 8 cut(s) 168, 351, 386, 473, 606, 849, 1083, 1086
FspBI CTAG 1 cut(s) 1047
GlaI GCGC 1 cut(s) 295
GluI GCNGC 8 cut(s) 168, 351, 386, 473, 606, 849, 1083, 1086
GsuI CTGGAG 1 cut(s) 1137
HaeIII GGCC 3 cut(s) 549, 697, 815
HapII CCGG 1 cut(s) 225
HhaI GCGC 1 cut(s) 296
Hin1I GRCGYC 1 cut(s) 753
Hin1II CATG 3 cut(s) 586, 1032, 1158
Hin6I GCGC 1 cut(s) 294
HinP1I GCGC 1 cut(s) 294
HindIII AAGCTT 1 cut(s) 525
HinfI GANTC 6 cut(s) 137, 231, 246, 271, 305, 366
HpaII CCGG 1 cut(s) 225
HphI GGTGA 2 cut(s) 272, 590
Hpy166II GTNNAC 2 cut(s) 158, 1054
Hpy188I TCNGA 6 cut(s) 99, 148, 238, 365, 711, 1129
Hpy188III TCNNGA 6 cut(s) 225, 302, 416, 453, 614, 829
Hpy8I GTNNAC 2 cut(s) 158, 1054
Hpy99I CGWCG 1 cut(s) 758
HpyAV CCTTC 2 cut(s) 826, 1050
HpyCH4III ACNGT 4 cut(s) 162, 332, 971, 1000
HpyCH4IV ACGT 2 cut(s) 18, 753
HpyCH4V TGCA 9 cut(s) 170, 266, 433, 442, 491, 605, 851, 977, 1032
HpyF10VI GCNNNNNNNGC 5 cut(s) 55, 341, 350, 439, 951
HpyF3I CTNAG 4 cut(s) 290, 741, 827, 1126
HpySE526I ACGT 2 cut(s) 18, 753
Hsp92I GRCGYC 1 cut(s) 753
Hsp92II CATG 3 cut(s) 586, 1032, 1158
HspAI GCGC 1 cut(s) 294
Kpn2I TCCGGA 1 cut(s) 224
Kzo9I GATC 8 cut(s) 81, 455, 556, 671, 681, 835, 1129, 1170
LmnI GCTCC 4 cut(s) 665, 802, 927, 1118
Lsp1109I GCAGC 7 cut(s) 154, 337, 484, 617, 835, 1072, 1094
LweI GCATC 1 cut(s) 179
MaeI CTAG 1 cut(s) 1047
MaeII ACGT 2 cut(s) 18, 753
MaeIII GTNAC 4 cut(s) 14, 278, 578, 1157
MalI GATC 8 cut(s) 83, 457, 558, 673, 683, 837, 1131, 1172
MboI GATC 8 cut(s) 81, 455, 556, 671, 681, 835, 1129, 1170
MboII GAAGA 5 cut(s) 115, 167, 240, 255, 830
MflI RGATCY 3 cut(s) 681, 835, 1129
MhlI GDGCHC 2 cut(s) 924, 1069
MlsI TGGCCA 1 cut(s) 549
MluCI AATT 5 cut(s) 142, 319, 337, 443, 570
MluNI TGGCCA 1 cut(s) 549
MlyI GAGTC 4 cut(s) 131, 240, 280, 360
MnlI CCTC 3 cut(s) 470, 586, 1072
Mox20I TGGCCA 1 cut(s) 549
MroI TCCGGA 1 cut(s) 224
MroXI GAANNNNTTC 1 cut(s) 515
MscI TGGCCA 1 cut(s) 549
MseI TTAA 5 cut(s) 446, 497, 635, 645, 795
Msp20I TGGCCA 1 cut(s) 549
MspA1I CMGCKG 2 cut(s) 740, 1085
MspI CCGG 1 cut(s) 225
MspR9I CCNGG 1 cut(s) 800
MvaI CCWGG 1 cut(s) 800
MwoI GCNNNNNNNGC 5 cut(s) 55, 341, 350, 439, 951
NdeII GATC 8 cut(s) 81, 455, 556, 671, 681, 835, 1129, 1170
NlaIII CATG 3 cut(s) 586, 1032, 1158
NlaIV GGNNCC 2 cut(s) 683, 900
NmuCI GTSAC 3 cut(s) 278, 578, 1157
NspI RCATGY 1 cut(s) 1158
PdmI GAANNNNTTC 1 cut(s) 515
PfeI GAWTC 2 cut(s) 231, 305
PflMI CCANNNNNTGG 1 cut(s) 395
PkrI GCNGC 8 cut(s) 169, 352, 387, 474, 607, 850, 1084, 1087
PleI GAGTC 4 cut(s) 131, 240, 279, 360
PpsI GAGTC 4 cut(s) 131, 240, 279, 360
Psp124BI GAGCTC 1 cut(s) 924
Psp6I CCWGG 1 cut(s) 798
PspEI GGTNACC 1 cut(s) 278
PspGI CCWGG 1 cut(s) 798
PspN4I GGNNCC 2 cut(s) 683, 900
PspPI GGNCC 1 cut(s) 695
PstNI CAGNNNCTG 3 cut(s) 152, 439, 805
PsuI RGATCY 3 cut(s) 681, 835, 1129
PvuII CAGCTG 2 cut(s) 740, 1085
RsaI GTAC 4 cut(s) 8, 515, 884, 959
RsaNI GTAC 4 cut(s) 7, 514, 883, 958
SacI GAGCTC 1 cut(s) 924
SaqAI TTAA 5 cut(s) 446, 497, 635, 645, 795
SatI GCNGC 8 cut(s) 168, 351, 386, 473, 606, 849, 1083, 1086
Sau3AI GATC 8 cut(s) 81, 455, 556, 671, 681, 835, 1129, 1170
Sau96I GGNCC 1 cut(s) 695
ScaI AGTACT 3 cut(s) 8, 515, 884
SchI GAGTC 4 cut(s) 131, 240, 280, 360
ScrFI CCNGG 1 cut(s) 800
SduI GDGCHC 2 cut(s) 924, 1069
SfaNI GCATC 1 cut(s) 179
Sse9I AATT 5 cut(s) 142, 319, 337, 443, 570
SsiI CCGC 1 cut(s) 386
SspI AATATT 3 cut(s) 486, 643, 791
SspMI CTAG 1 cut(s) 1047
SstI GAGCTC 1 cut(s) 924
StyD4I CCNGG 1 cut(s) 798
StyI CCWWGG 2 cut(s) 90, 504
TaaI ACNGT 4 cut(s) 162, 332, 971, 1000
TaiI ACGT 2 cut(s) 21, 756
TaqI TCGA 2 cut(s) 255, 540
TasI AATT 5 cut(s) 142, 319, 337, 443, 570
TatI WGTACW 4 cut(s) 6, 513, 882, 957
TauI GCSGC 1 cut(s) 388
TfiI GAWTC 2 cut(s) 231, 305
Tru1I TTAA 5 cut(s) 446, 497, 635, 645, 795
Tru9I TTAA 5 cut(s) 446, 497, 635, 645, 795
TscAI CASTG 4 cut(s) 352, 396, 631, 933
TseFI GTSAC 3 cut(s) 278, 578, 1157
TseI GCWGC 7 cut(s) 167, 350, 472, 605, 848, 1082, 1085
Tsp45I GTSAC 3 cut(s) 278, 578, 1157
TspDTI ATGAA 3 cut(s) 599, 792, 1017
TspGWI ACGGA 1 cut(s) 203
TspRI CASTG 4 cut(s) 352, 396, 631, 933
Van91I CCANNNNNTGG 1 cut(s) 395
XapI RAATTY 3 cut(s) 142, 319, 570
XceI RCATGY 1 cut(s) 1158
XmnI GAANNNNTTC 1 cut(s) 515
XspI CTAG 1 cut(s) 1047
ZraI GACGTC 1 cut(s) 754
ZrmI AGTACT 3 cut(s) 8, 515, 884
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.