RchiOBHm_Chr6g0295771

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000978
Physical Location & Seq
Reverse (-)
57691987 .. 57694611
2625 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26545

Sequence Viewer

Length: 1173 bp
ATGGGGTACTCTCCTAAAGTTATCATTTCTCTGTTCTGCTCCTATATGCTTATATTGCATATTACATGTAGTGCTGTGATCCCTGCCTTGGCTTTGAAAGACTGTGAGTTTCCTGCCATCTTCAACTTCGGAGACTCAAATTCAGATACTGGTGGATTTGCCGCATCATCCCTTCCATCAACACCAACCCCACCTTATGGAGAGACCTATTTCCATATGCCGGCCGGAAGATTCTCTGATGGAAGACTCATCATCGATTTCCTTGCAAAGAGTCTTGGTCACCCTTATCTAAGCGCATATCTGGATTCTCTCGGGACAAATTTCTCGTATGGTGCAAATTTTGCAACTGCAGCTTCCACTATCAGACTCCCAGATTTCATTTTACCAGCCGGTGGATTTAGTCCCTTCTACCTTGATATTCAATATCTGCAGTTCCTGCAACTAAAATCCAGATCACAACTCATAAGGCGTCGAGGGGGAATATTTGCAAGTTTAATGCCCAAGGAGGAGTACTTTTCCAAAGCTTTATACACATTCGACACTGGCCAGAATGATCTTGGCGAGGGATTTTCCGGTAACCTGACTATACAGGAAGTAAATGCATCTGTTCCTGATATAATCACTGGTTTCTCAACGAATATCAAGAAAATATATGATTTGGGAGCAAGATCATTATGGATCCACAATACCGGGCCGATTGGCTGTCTCCCTTACATTCAGGCAAACTTCCCATCAGCTCAGAAAGATGAGATTGGCTGTGCAAAGGCTTACAATGAAGTAGCTGAGCATTTTAACCATGAGCTGAAGCAGGCCATAGTTCAACTCAGGAAGGATCTTCCTAAGGCTGCATTTACTTATGTAGATGTGTATTCTGTCAAATACTCTCTTTTCAAGGAACCCGAGAAATATGGATTTGAGCTCCCACTTGTTGCTTGTTGTGGCTACGGTGGTAAGTACAACTATAACGGCACTGCTGAATGTGGAGCAATAATTACAGTCAATGGAAGCCAAATCTTTGTTGGTTCATGCAAAAACCCCTCAACTAGAGTTGTCTGGGATGGTATTCATTACACCGAGGCAGCTGCAAAATTTATTTTTGATAAAATTTCAACTGGAGCATATTCAGATCCACCTCTTCCCTTGAAACAAGCATGTCAAAGGAGTTTGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005488 GO:0006950 GO:0006952 GO:0008150 GO:0016787 GO:0016788 GO:0019863 GO:0019865 GO:0044877 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

390

Amino Acids

42.81

Weight (kDa)

6.65

Isoelectric Point (pI)

40.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 40 - 369 2.4e-46 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 197, 392
AciI CCGC 1 cut(s) 162
AclWI GGATC 5 cut(s) 73, 673, 686, 840, 1121
AcoI YGGCCR 2 cut(s) 222, 544
AcsI RAATTY 5 cut(s) 139, 319, 337, 1088, 1104
AcuI CTGAAG 1 cut(s) 824
AcyI GRCGYC 1 cut(s) 469
AfaI GTAC 3 cut(s) 8, 512, 956
AfiI CCNNNNNNNGG 4 cut(s) 88, 197, 220, 392
AflIII ACRYGT 1 cut(s) 65
AgsI TTSAA 8 cut(s) 97, 124, 422, 821, 892, 1110, 1144, 1168
AluBI AGCT 7 cut(s) 353, 524, 737, 782, 802, 919, 1082
AluI AGCT 7 cut(s) 353, 524, 737, 782, 802, 919, 1082
Alw21I GWGCWC 1 cut(s) 921
Alw26I GTCTC 3 cut(s) 126, 197, 710
AlwI GGATC 5 cut(s) 73, 673, 686, 840, 1121
AlwNI CAGNNNCTG 2 cut(s) 149, 436
Ama87I CYCGRG 2 cut(s) 311, 899
AoxI GGCC 4 cut(s) 222, 544, 692, 810
ApeKI GCWGC 4 cut(s) 350, 845, 1079, 1082
ApoI RAATTY 5 cut(s) 139, 319, 337, 1088, 1104
AspLEI GCGC 1 cut(s) 296
AspS9I GGNCC 1 cut(s) 692
AsuC2I CCSGG 1 cut(s) 691
AsuHPI GGTGA 1 cut(s) 272
AvaI CYCGRG 2 cut(s) 311, 899
AxyI CCTNAGG 1 cut(s) 840
BalI TGGCCA 1 cut(s) 546
BamHI GGATCC 1 cut(s) 678
BanII GRGCYC 1 cut(s) 921
BbsI GAAGAC 1 cut(s) 250
Bbv12I GWGCWC 1 cut(s) 921
BbvI GCAGC 4 cut(s) 362, 832, 1069, 1091
BccI CCATC 5 cut(s) 125, 184, 233, 739, 1052
BceAI ACGGC 1 cut(s) 982
BcgI CGANNNNNNTGC 4 cut(s) 245, 279, 1064, 1098
BcnI CCSGG 1 cut(s) 691
BcoDI GTCTC 3 cut(s) 126, 197, 710
BfaI CTAG 1 cut(s) 1044
BfmI CTRYAG 2 cut(s) 348, 428
BisI GCNGC 5 cut(s) 162, 351, 846, 1080, 1083
BlpI GCTNAGC 1 cut(s) 783
BlsI GCNGC 5 cut(s) 163, 352, 847, 1081, 1084
BmcAI AGTACT 1 cut(s) 512
Bme1390I CCNGG 1 cut(s) 691
BmeT110I CYCGRG 2 cut(s) 311, 899
BmgT120I GGNCC 1 cut(s) 692
BmiI GGNNCC 2 cut(s) 680, 897
BmrFI CCNGG 1 cut(s) 691
BmsI GCATC 2 cut(s) 173, 611
BpiI GAAGAC 1 cut(s) 250
BpmI CTGGAG 1 cut(s) 1134
Bpu1102I GCTNAGC 1 cut(s) 783
BpuMI CCSGG 1 cut(s) 691
Bsa29I ATCGAT 1 cut(s) 255
BsaHI GRCGYC 1 cut(s) 469
BsaI GGTCTC 1 cut(s) 197
BsaJI CCNNGG 3 cut(s) 87, 501, 1074
BsaWI WCCGGW 1 cut(s) 572
Bsc4I CCNNNNNNNGG 4 cut(s) 88, 197, 220, 392
Bse118I RCCGGY 2 cut(s) 220, 389
Bse1I ACTGG 4 cut(s) 154, 547, 628, 1117
Bse21I CCTNAGG 1 cut(s) 840
BseCI ATCGAT 1 cut(s) 255
BseDI CCNNGG 3 cut(s) 87, 501, 1074
BseGI GGATG 2 cut(s) 167, 1063
BseLI CCNNNNNNNGG 4 cut(s) 88, 197, 220, 392
BseMII CTCAG 3 cut(s) 752, 774, 838
BseNI ACTGG 4 cut(s) 154, 547, 628, 1117
BseRI GAGGAG 1 cut(s) 521
BseX3I CGGCCG 1 cut(s) 222
BseXI GCAGC 4 cut(s) 362, 832, 1069, 1091
Bsh1285I CGRYCG 1 cut(s) 225
BshFI GGCC 4 cut(s) 224, 546, 694, 812
BshVI ATCGAT 1 cut(s) 255
BsiEI CGRYCG 1 cut(s) 225
BsiHKAI GWGCWC 1 cut(s) 921
BsiHKCI CYCGRG 2 cut(s) 311, 899
BsiSI CCGG 5 cut(s) 221, 225, 390, 573, 690
BslFI GGGAC 2 cut(s) 328, 387
BslI CCNNNNNNNGG 4 cut(s) 88, 197, 220, 392
BsmAI GTCTC 3 cut(s) 126, 197, 710
BsmFI GGGAC 2 cut(s) 328, 387
BsnI GGCC 4 cut(s) 224, 546, 694, 812
Bso31I GGTCTC 1 cut(s) 197
BsoBI CYCGRG 2 cut(s) 311, 899
Bsp1286I GDGCHC 1 cut(s) 921
Bsp143I GATC 7 cut(s) 78, 452, 553, 668, 678, 832, 1126
Bsp1720I GCTNAGC 1 cut(s) 783
BspACI CCGC 1 cut(s) 162
BspANI GGCC 4 cut(s) 224, 546, 694, 812
BspCNI CTCAG 3 cut(s) 751, 775, 837
BspDI ATCGAT 1 cut(s) 255
BspLI GGNNCC 2 cut(s) 680, 897
BspMAI CTGCAG 2 cut(s) 352, 432
BspPI GGATC 5 cut(s) 73, 673, 686, 840, 1121
BspTNI GGTCTC 1 cut(s) 197
BsrFI RCCGGY 2 cut(s) 220, 389
BsrI ACTGG 4 cut(s) 154, 547, 628, 1117
BssAI RCCGGY 2 cut(s) 220, 389
BssECI CCNNGG 3 cut(s) 87, 501, 1074
BssMI GATC 7 cut(s) 78, 452, 553, 668, 678, 832, 1126
BssNI GRCGYC 1 cut(s) 469
BssT1I CCWWGG 2 cut(s) 87, 501
Bst4CI ACNGT 3 cut(s) 104, 947, 997
Bst6I CTCTTC 1 cut(s) 1140
BstACI GRCGYC 1 cut(s) 469
BstAPI GCANNNNNTGC 2 cut(s) 341, 436
BstC8I GCNNGC 2 cut(s) 222, 810
BstDEI CTNAG 5 cut(s) 290, 738, 783, 824, 840
BstEII GGTNACC 2 cut(s) 278, 575
BstF5I GGATG 2 cut(s) 167, 1063
BstHHI GCGC 1 cut(s) 296
BstKTI GATC 7 cut(s) 81, 455, 556, 671, 681, 835, 1129
BstMAI GTCTC 3 cut(s) 126, 197, 710
BstMBI GATC 7 cut(s) 78, 452, 553, 668, 678, 832, 1126
BstMCI CGRYCG 1 cut(s) 225
BstMWI GCNNNNNNNGC 4 cut(s) 55, 341, 350, 436
BstNSI RCATGY 2 cut(s) 69, 1155
BstPI GGTNACC 2 cut(s) 278, 575
BstSCI CCNGG 1 cut(s) 689
BstSFI CTRYAG 2 cut(s) 348, 428
BstV1I GCAGC 4 cut(s) 362, 832, 1069, 1091
BstV2I GAAGAC 1 cut(s) 250
BstX2I RGATCY 3 cut(s) 678, 832, 1126
BstYI RGATCY 3 cut(s) 678, 832, 1126
BstZI CGGCCG 1 cut(s) 222
Bsu15I ATCGAT 1 cut(s) 255
Bsu36I CCTNAGG 1 cut(s) 840
BsuRI GGCC 4 cut(s) 224, 546, 694, 812
BsuTUI ATCGAT 1 cut(s) 255
BtsCI GGATG 2 cut(s) 167, 1063
BtsI GCAGTG 1 cut(s) 969
BtsIMutI CAGTG 3 cut(s) 540, 621, 969
Cac8I GCNNGC 2 cut(s) 222, 810
CaiI CAGNNNCTG 2 cut(s) 149, 436
CfoI GCGC 1 cut(s) 296
Cfr10I RCCGGY 2 cut(s) 220, 389
Cfr13I GGNCC 1 cut(s) 692
ClaI ATCGAT 1 cut(s) 255
CseI GACGC 1 cut(s) 458
Csp6I GTAC 3 cut(s) 7, 511, 955
CviAII CATG 4 cut(s) 66, 797, 1026, 1152
CviQI GTAC 3 cut(s) 7, 511, 955
DdeI CTNAG 5 cut(s) 290, 738, 783, 824, 840
DpnI GATC 7 cut(s) 80, 454, 555, 670, 680, 834, 1128
DpnII GATC 7 cut(s) 78, 452, 553, 668, 678, 832, 1126
EaeI YGGCCR 2 cut(s) 222, 544
EagI CGGCCG 1 cut(s) 222
Eam1104I CTCTTC 1 cut(s) 1140
EarI CTCTTC 1 cut(s) 1140
Ecl136II GAGCTC 1 cut(s) 919
EclXI CGGCCG 1 cut(s) 222
Eco130I CCWWGG 2 cut(s) 87, 501
Eco24I GRGCYC 1 cut(s) 921
Eco31I GGTCTC 1 cut(s) 197
Eco52I CGGCCG 1 cut(s) 222
Eco53kI GAGCTC 1 cut(s) 919
Eco57I CTGAAG 1 cut(s) 824
Eco81I CCTNAGG 1 cut(s) 840
Eco88I CYCGRG 2 cut(s) 311, 899
Eco91I GGTNACC 2 cut(s) 278, 575
EcoICRI GAGCTC 1 cut(s) 919
EcoO65I GGTNACC 2 cut(s) 278, 575
EcoT14I CCWWGG 2 cut(s) 87, 501
EcoT22I ATGCAT 1 cut(s) 604
EcoT38I GRGCYC 1 cut(s) 921
ErhI CCWWGG 2 cut(s) 87, 501
FaeI CATG 4 cut(s) 69, 800, 1029, 1155
FaqI GGGAC 2 cut(s) 328, 387
FatI CATG 4 cut(s) 65, 796, 1025, 1151
FauNDI CATATG 1 cut(s) 216
Fnu4HI GCNGC 5 cut(s) 162, 351, 846, 1080, 1083
FokI GGATG 2 cut(s) 154, 1070
FriOI GRGCYC 1 cut(s) 921
Fsp4HI GCNGC 5 cut(s) 162, 351, 846, 1080, 1083
FspBI CTAG 1 cut(s) 1044
GlaI GCGC 1 cut(s) 295
GluI GCNGC 5 cut(s) 162, 351, 846, 1080, 1083
GsuI CTGGAG 1 cut(s) 1134
HaeIII GGCC 4 cut(s) 224, 546, 694, 812
HapII CCGG 5 cut(s) 221, 225, 390, 573, 690
HgaI GACGC 1 cut(s) 458
HhaI GCGC 1 cut(s) 296
Hin1I GRCGYC 1 cut(s) 469
Hin1II CATG 4 cut(s) 69, 800, 1029, 1155
Hin6I GCGC 1 cut(s) 294
HinP1I GCGC 1 cut(s) 294
HindIII AAGCTT 1 cut(s) 522
HinfI GANTC 6 cut(s) 134, 231, 246, 271, 305, 366
HpaII CCGG 5 cut(s) 221, 225, 390, 573, 690
HphI GGTGA 1 cut(s) 272
Hpy188I TCNGA 6 cut(s) 131, 145, 238, 365, 741, 1126
Hpy188III TCNNGA 6 cut(s) 302, 313, 450, 611, 643, 826
Hpy99I CGWCG 1 cut(s) 474
HpyAV CCTTC 3 cut(s) 182, 415, 823
HpyCH4III ACNGT 3 cut(s) 104, 947, 997
HpyF10VI GCNNNNNNNGC 4 cut(s) 55, 341, 350, 436
HpyF3I CTNAG 5 cut(s) 290, 738, 783, 824, 840
Hsp92I GRCGYC 1 cut(s) 469
Hsp92II CATG 4 cut(s) 69, 800, 1029, 1155
HspAI GCGC 1 cut(s) 294
KroI GCCGGC 1 cut(s) 220
KroNI GCCGGC 1 cut(s) 222
Kzo9I GATC 7 cut(s) 78, 452, 553, 668, 678, 832, 1126
LmnI GCTCC 5 cut(s) 44, 662, 924, 983, 1115
Lsp1109I GCAGC 4 cut(s) 362, 832, 1069, 1091
LweI GCATC 2 cut(s) 173, 611
MaeI CTAG 1 cut(s) 1044
MaeIII GTNAC 2 cut(s) 278, 575
MalI GATC 7 cut(s) 80, 454, 555, 670, 680, 834, 1128
MboI GATC 7 cut(s) 78, 452, 553, 668, 678, 832, 1126
MboII GAAGA 5 cut(s) 112, 240, 255, 827, 1127
MflI RGATCY 3 cut(s) 678, 832, 1126
MhlI GDGCHC 1 cut(s) 921
MlsI TGGCCA 1 cut(s) 546
MluCI AATT 6 cut(s) 139, 319, 337, 990, 1088, 1104
MluNI TGGCCA 1 cut(s) 546
MlyI GAGTC 4 cut(s) 128, 240, 280, 360
MnlI CCTC 6 cut(s) 467, 499, 556, 1048, 1069, 1143
Mox20I TGGCCA 1 cut(s) 546
Mph1103I ATGCAT 1 cut(s) 604
MroNI GCCGGC 1 cut(s) 220
MscI TGGCCA 1 cut(s) 546
MseI TTAA 2 cut(s) 494, 792
Msp20I TGGCCA 1 cut(s) 546
MspA1I CMGCKG 1 cut(s) 1082
MspI CCGG 5 cut(s) 221, 225, 390, 573, 690
MspR9I CCNGG 1 cut(s) 691
MwoI GCNNNNNNNGC 4 cut(s) 55, 341, 350, 436
NaeI GCCGGC 1 cut(s) 222
NciI CCSGG 1 cut(s) 691
NdeI CATATG 1 cut(s) 216
NdeII GATC 7 cut(s) 78, 452, 553, 668, 678, 832, 1126
NgoMIV GCCGGC 1 cut(s) 220
NlaIII CATG 4 cut(s) 69, 800, 1029, 1155
NlaIV GGNNCC 2 cut(s) 680, 897
NmuCI GTSAC 1 cut(s) 278
NsiI ATGCAT 1 cut(s) 604
NspI RCATGY 2 cut(s) 69, 1155
PciI ACATGT 1 cut(s) 65
PdiI GCCGGC 1 cut(s) 222
PfeI GAWTC 2 cut(s) 231, 305
PflMI CCANNNNNTGG 2 cut(s) 197, 392
PkrI GCNGC 5 cut(s) 163, 352, 847, 1081, 1084
PleI GAGTC 4 cut(s) 128, 240, 279, 360
PpsI GAGTC 4 cut(s) 128, 240, 279, 360
PscI ACATGT 1 cut(s) 65
Psp124BI GAGCTC 1 cut(s) 921
PspEI GGTNACC 2 cut(s) 278, 575
PspN4I GGNNCC 2 cut(s) 680, 897
PspPI GGNCC 1 cut(s) 692
PstI CTGCAG 2 cut(s) 352, 432
PstNI CAGNNNCTG 2 cut(s) 149, 436
PsuI RGATCY 3 cut(s) 678, 832, 1126
PvuII CAGCTG 1 cut(s) 1082
RsaI GTAC 3 cut(s) 8, 512, 956
RsaNI GTAC 3 cut(s) 7, 511, 955
SacI GAGCTC 1 cut(s) 921
SaqAI TTAA 2 cut(s) 494, 792
SatI GCNGC 5 cut(s) 162, 351, 846, 1080, 1083
Sau3AI GATC 7 cut(s) 78, 452, 553, 668, 678, 832, 1126
Sau96I GGNCC 1 cut(s) 692
ScaI AGTACT 1 cut(s) 512
SchI GAGTC 4 cut(s) 128, 240, 280, 360
ScrFI CCNGG 1 cut(s) 691
SduI GDGCHC 1 cut(s) 921
SfaNI GCATC 2 cut(s) 173, 611
SfcI CTRYAG 2 cut(s) 348, 428
Sse9I AATT 6 cut(s) 139, 319, 337, 990, 1088, 1104
SsiI CCGC 1 cut(s) 162
SspI AATATT 1 cut(s) 483
SspMI CTAG 1 cut(s) 1044
SstI GAGCTC 1 cut(s) 921
StyD4I CCNGG 1 cut(s) 689
StyI CCWWGG 2 cut(s) 87, 501
TaaI ACNGT 3 cut(s) 104, 947, 997
TaqI TCGA 3 cut(s) 255, 472, 537
TasI AATT 6 cut(s) 139, 319, 337, 990, 1088, 1104
TatI WGTACW 2 cut(s) 510, 954
TauI GCSGC 1 cut(s) 164
TfiI GAWTC 2 cut(s) 231, 305
Tru1I TTAA 2 cut(s) 494, 792
Tru9I TTAA 2 cut(s) 494, 792
TscAI CASTG 3 cut(s) 547, 628, 976
TseFI GTSAC 1 cut(s) 278
TseI GCWGC 4 cut(s) 350, 845, 1079, 1082
Tsp45I GTSAC 1 cut(s) 278
TspDTI ATGAA 4 cut(s) 367, 789, 1014, 1055
TspRI CASTG 3 cut(s) 547, 628, 976
Van91I CCANNNNNTGG 2 cut(s) 197, 392
XapI RAATTY 5 cut(s) 139, 319, 337, 1088, 1104
XceI RCATGY 2 cut(s) 69, 1155
XcmI CCANNNNNNNNNTGG 2 cut(s) 554, 1016
XspI CTAG 1 cut(s) 1044
ZrmI AGTACT 1 cut(s) 512
Zsp2I ATGCAT 1 cut(s) 604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.