Rorug06G0253900

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000978
Physical Location & Seq
Reverse (-)
40040321 .. 40042136
1816 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0253900.1

Sequence Viewer

Length: 1575 bp
ATGATTTATGTTAATGCTTCGTTGGAGGATTGCTATCAGATCCAAGATGTACTTGAGATGTATGGTAGAGCTTCAGGTCAGTTAGTTAACTTTACCAAGAGTTCTGTTGTTTTTAATAAAAATGTTACGGAATTTATGAGAGAAGAAATTACTAGTTTTCTGGAGGTGGAGGAAGTGGAATCTAATGAGAAATATTTGGGATTGCCAACCTATGTTGGAAGAAAGAAAACTTCTACGTTCCAATACATAAAAGATAATTTGGCGAAGAAGCTAGCAATTTGGCAAGGAAAGTTATTAAGCGGGGCTGGAAAGGATATTCTCATGACCAGGGTGGTTGCTCAAGCATTGCCTACTTATGCAATGAGTGTTTTCCAGCTCACTAAGAATTTTTGTGAGGACCTTGAGCAAATGTGTGCTCGTTTTTGGTGGGGGAGTACTCTTGATAAGAGCAAAATTCATTGGAAAACTTGGAAGGCTTTGTGTAACCCAAAGGAGGAGGGTGGCCTAGGGTTTTGTAGTTTGTCAAATTTTAATACTGCAATGTTGGCAAAACAATCATGGCGTGTGGTGAACAATCCGACTTCGTTAGTGGCTCGGATCTATAAAGCAAAATACTATCCAGATACTACCTTTTGGTTAACTGAGGCTTCTGCTTCTCCTTCCTTTTCTTGGAGAAGTCTATTTTCTACAAGAGAATTTTTGAGACAAAGCTCGTATTGGCAGATCGGTAATGGTTTGTCTGTTGATATTTGGTCAGATTGTTGGGTGCCTGGAGTCCCAGATTACAAACCGGTTGATAATGGTATAGCGTCGTTGGAGGTTAGACAAGTAAGTGAGTTGTTGAACCAAGCAGGGAGGTGGAATGAGCTATTGATTAGACAAATGTTTCCAATGAAGGAAGCTGAAGCTATTTTGAGTATACCTATAAGTTCTAGAGTTGTTGACGATAGAGTGGTGTGGCGTATGGAAAAGAATGGTAAATTCACGGTTAAGTCAGCATACCGTCAGGATTTTTCTATGTCAAACTCTAGAAGTCCTTTTCAAGTTTCAGTGGGGGTTAACTTTTGGAAGAAGATTTGGAAAGTTGTCATCCCTAATTCTGCCAAGGTTCATATATGGAGGGTTTGCCATAATATTTTACCCTCTCTTGAGAGACTTGCGTCAAAAAGGGTGGTGTTGGAAACACAGATTTGGGATAATAAGAGTGTTACAACTTCTGAAGTGCTTCTAATGTCCATGACTAGGCTGCATGCCTTTAGATTTCATAATTCAAAACCTCATATTGGAGGTACTGTGACGTTGTCACGTTGGTCTTCTCCTCTTGTGGGATGGTACAAGATTAATGTAGATGGTTCCTATAATTATACTTCAAAATGTGGAGGAGCGGGTTTTGTAGTCAGGGATAGCTTGGGAAACTTTCTTGCTGGGGAAGGTAGGCCATTGCAGGGGTTATTATGCTCGGAGCATGCTGAGGTGTTGGGCTGCAGATTGGCAGCGAACTTTGCTGTGGAGCAGGGGTTTGTTCCTTCTTTATTGGAGCTCGATGCTCAGGTGGTACAGCGGCAATTGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005488 GO:0006950 GO:0006952 GO:0008150 GO:0016787 GO:0016788 GO:0019863 GO:0019865 GO:0044877 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

524

Amino Acids

59.6

Weight (kDa)

9.15

Isoelectric Point (pI)

36.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 448 - 523 1.7e-09 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 766
AccBSI CCGCTC 1 cut(s) 1385
AccI GTMKAC 1 cut(s) 919
AciI CCGC 3 cut(s) 300, 1385, 1561
AclWI GGATC 2 cut(s) 34, 605
AcsI RAATTY 6 cut(s) 131, 385, 453, 526, 695, 980
AcuI CTGAAG 3 cut(s) 57, 924, 1239
AfaI GTAC 5 cut(s) 51, 436, 1291, 1334, 1557
AfiI CCNNNNNNNGG 6 cut(s) 493, 669, 1242, 1283, 1325, 1445
AgeI ACCGGT 1 cut(s) 790
AgsI TTSAA 4 cut(s) 844, 1043, 1272, 1371
AhlI ACTAGT 1 cut(s) 152
AjnI CCWGG 2 cut(s) 326, 769
AluBI AGCT 9 cut(s) 71, 271, 376, 711, 868, 902, 908, 1407, 1540
AluI AGCT 9 cut(s) 71, 271, 376, 711, 868, 902, 908, 1407, 1540
Alw21I GWGCWC 2 cut(s) 418, 1542
Alw26I GTCTC 2 cut(s) 697, 1147
AlwI GGATC 2 cut(s) 34, 605
AoxI GGCC 2 cut(s) 502, 1436
ApeKI GCWGC 3 cut(s) 1246, 1482, 1493
ApoI RAATTY 6 cut(s) 131, 385, 453, 526, 695, 980
AseI ATTAAT 1 cut(s) 1341
AsiGI ACCGGT 1 cut(s) 790
Asp700I GAANNNNTTC 1 cut(s) 1224
AspA2I CCTAGG 1 cut(s) 505
AspS9I GGNCC 1 cut(s) 397
AsuHPI GGTGA 1 cut(s) 580
AsuNHI GCTAGC 1 cut(s) 271
AvaII GGWCC 1 cut(s) 397
AvrII CCTAGG 1 cut(s) 505
BanI GGYRCC 1 cut(s) 766
BanII GRGCYC 1 cut(s) 1542
BbsI GAAGAC 1 cut(s) 1305
Bbv12I GWGCWC 2 cut(s) 418, 1542
BbvCI CCTCAGC 1 cut(s) 1470
BbvI GCAGC 3 cut(s) 1233, 1469, 1505
BccI CCATC 2 cut(s) 1323, 1343
BciT130I CCWGG 2 cut(s) 328, 771
BcoDI GTCTC 2 cut(s) 697, 1147
BcuI ACTAGT 1 cut(s) 152
BfaI CTAG 6 cut(s) 153, 272, 506, 933, 1029, 1242
BfmI CTRYAG 1 cut(s) 1483
BisI GCNGC 4 cut(s) 1247, 1483, 1494, 1562
BlnI CCTAGG 1 cut(s) 505
BlsI GCNGC 4 cut(s) 1248, 1484, 1495, 1563
BmcAI AGTACT 1 cut(s) 436
Bme1390I CCNGG 2 cut(s) 328, 771
Bme18I GGWCC 1 cut(s) 397
BmgT120I GGNCC 1 cut(s) 397
BmiI GGNNCC 2 cut(s) 768, 1354
BmrFI CCNGG 2 cut(s) 328, 771
BmsI GCATC 1 cut(s) 1534
BmtI GCTAGC 1 cut(s) 275
BoxI GACNNNNGTC 1 cut(s) 1159
BpiI GAAGAC 1 cut(s) 1305
BpmI CTGGAG 2 cut(s) 182, 792
Bpu10I CCTNAGC 2 cut(s) 1470, 1548
BpuEI CTTGAG 4 cut(s) 74, 324, 422, 1169
BsaBI GATNNNNATC 1 cut(s) 33
BsaJI CCNNGG 3 cut(s) 327, 505, 1104
BsaWI WCCGGW 1 cut(s) 790
BsaXI ACNNNNNCTCC 4 cut(s) 1278, 1308, 1502, 1532
Bsc4I CCNNNNNNNGG 6 cut(s) 493, 669, 1242, 1283, 1325, 1445
Bse118I RCCGGY 1 cut(s) 790
Bse3DI GCAATG 4 cut(s) 344, 366, 546, 1439
Bse8I GATNNNNATC 1 cut(s) 33
BseBI CCWGG 2 cut(s) 328, 771
BseDI CCNNGG 3 cut(s) 327, 505, 1104
BseGI GGATG 2 cut(s) 1089, 1334
BseJI GATNNNNATC 1 cut(s) 33
BseLI CCNNNNNNNGG 6 cut(s) 493, 669, 1242, 1283, 1325, 1445
BseMI GCAATG 4 cut(s) 344, 366, 546, 1439
BseMII CTCAG 3 cut(s) 633, 1461, 1562
BseRI GAGGAG 3 cut(s) 509, 1308, 1395
BseXI GCAGC 3 cut(s) 1233, 1469, 1505
BseYI CCCAGC 1 cut(s) 1424
BshFI GGCC 2 cut(s) 504, 1438
BshNI GGYRCC 1 cut(s) 766
BshTI ACCGGT 1 cut(s) 790
BsiHKAI GWGCWC 2 cut(s) 418, 1542
BsiSI CCGG 1 cut(s) 791
BslFI GGGAC 1 cut(s) 761
BslI CCNNNNNNNGG 6 cut(s) 493, 669, 1242, 1283, 1325, 1445
BsmAI GTCTC 2 cut(s) 697, 1147
BsmFI GGGAC 1 cut(s) 761
BsnI GGCC 2 cut(s) 504, 1438
Bsp1286I GDGCHC 2 cut(s) 418, 1542
Bsp143I GATC 3 cut(s) 39, 597, 723
BspACI CCGC 3 cut(s) 300, 1385, 1561
BspANI GGCC 2 cut(s) 504, 1438
BspCNI CTCAG 3 cut(s) 634, 1462, 1561
BspHI TCATGA 1 cut(s) 321
BspLI GGNNCC 2 cut(s) 768, 1354
BspMAI CTGCAG 1 cut(s) 1487
BspOI GCTAGC 1 cut(s) 275
BspPI GGATC 2 cut(s) 34, 605
BspT107I GGYRCC 1 cut(s) 766
BsrBI CCGCTC 1 cut(s) 1385
BsrDI GCAATG 4 cut(s) 344, 366, 546, 1439
BsrFI RCCGGY 1 cut(s) 790
BssAI RCCGGY 1 cut(s) 790
BssECI CCNNGG 3 cut(s) 327, 505, 1104
BssMI GATC 3 cut(s) 39, 597, 723
BssNAI GTATAC 1 cut(s) 920
BssT1I CCWWGG 2 cut(s) 505, 1104
Bst1107I GTATAC 1 cut(s) 920
Bst2UI CCWGG 2 cut(s) 328, 771
Bst4CI ACNGT 3 cut(s) 988, 1004, 1294
BstC8I GCNNGC 3 cut(s) 273, 1251, 1467
BstDEI CTNAG 4 cut(s) 381, 642, 1470, 1548
BstF5I GGATG 2 cut(s) 1089, 1334
BstKTI GATC 3 cut(s) 42, 600, 726
BstMAI GTCTC 2 cut(s) 697, 1147
BstMBI GATC 3 cut(s) 39, 597, 723
BstMWI GCNNNNNNNGC 2 cut(s) 545, 1502
BstNI CCWGG 2 cut(s) 328, 771
BstNSI RCATGY 2 cut(s) 1253, 1469
BstPAI GACNNNNGTC 1 cut(s) 1159
BstSCI CCNGG 2 cut(s) 326, 769
BstSFI CTRYAG 1 cut(s) 1483
BstV1I GCAGC 3 cut(s) 1233, 1469, 1505
BstV2I GAAGAC 1 cut(s) 1305
BstX2I RGATCY 2 cut(s) 39, 597
BstYI RGATCY 2 cut(s) 39, 597
BstZ17I GTATAC 1 cut(s) 920
BsuRI GGCC 2 cut(s) 504, 1438
BtsCI GGATG 2 cut(s) 1089, 1334
BtsIMutI CAGTG 1 cut(s) 1056
Cac8I GCNNGC 3 cut(s) 273, 1251, 1467
CciI TCATGA 1 cut(s) 321
Cfr10I RCCGGY 1 cut(s) 790
Cfr13I GGNCC 1 cut(s) 397
CseI GACGC 2 cut(s) 798, 1149
Csp6I GTAC 5 cut(s) 50, 435, 1290, 1333, 1556
CspAI ACCGGT 1 cut(s) 790
CspCI CAANNNNNGTGG 2 cut(s) 1152, 1187
CviAII CATG 5 cut(s) 322, 558, 1237, 1250, 1466
CviQI GTAC 5 cut(s) 50, 435, 1290, 1333, 1556
DdeI CTNAG 4 cut(s) 381, 642, 1470, 1548
DpnI GATC 3 cut(s) 41, 599, 725
DpnII GATC 3 cut(s) 39, 597, 723
Ecl136II GAGCTC 1 cut(s) 1540
Eco130I CCWWGG 2 cut(s) 505, 1104
Eco24I GRGCYC 1 cut(s) 1542
Eco47I GGWCC 1 cut(s) 397
Eco53kI GAGCTC 1 cut(s) 1540
Eco57I CTGAAG 3 cut(s) 57, 924, 1239
EcoICRI GAGCTC 1 cut(s) 1540
EcoO109I RGGNCCY 1 cut(s) 397
EcoRII CCWGG 2 cut(s) 326, 769
EcoT14I CCWWGG 2 cut(s) 505, 1104
EcoT38I GRGCYC 1 cut(s) 1542
ErhI CCWWGG 2 cut(s) 505, 1104
FaeI CATG 5 cut(s) 325, 561, 1240, 1253, 1469
FalI AAGNNNNNCTT 2 cut(s) 36, 68
FaqI GGGAC 1 cut(s) 761
FatI CATG 5 cut(s) 321, 557, 1236, 1249, 1465
FauI CCCGC 2 cut(s) 293, 1378
FblI GTMKAC 1 cut(s) 919
Fnu4HI GCNGC 4 cut(s) 1247, 1483, 1494, 1562
FokI GGATG 2 cut(s) 1076, 1341
FriOI GRGCYC 1 cut(s) 1542
Fsp4HI GCNGC 4 cut(s) 1247, 1483, 1494, 1562
FspBI CTAG 6 cut(s) 153, 272, 506, 933, 1029, 1242
GluI GCNGC 4 cut(s) 1247, 1483, 1494, 1562
GsaI CCCAGC 1 cut(s) 1428
GsuI CTGGAG 2 cut(s) 182, 792
HaeIII GGCC 2 cut(s) 504, 1438
HapII CCGG 1 cut(s) 791
HgaI GACGC 2 cut(s) 798, 1149
Hin1II CATG 5 cut(s) 325, 561, 1240, 1253, 1469
HincII GTYRAC 4 cut(s) 88, 639, 943, 1060
HindII GTYRAC 4 cut(s) 88, 639, 943, 1060
HinfI GANTC 2 cut(s) 179, 774
HpaI GTTAAC 3 cut(s) 88, 639, 1060
HpaII CCGG 1 cut(s) 791
HphI GGTGA 1 cut(s) 580
Hpy166II GTNNAC 6 cut(s) 88, 571, 639, 920, 943, 1060
Hpy188I TCNGA 6 cut(s) 39, 579, 597, 757, 1219, 1462
Hpy188III TCNNGA 8 cut(s) 161, 322, 440, 620, 933, 1007, 1029, 1148
Hpy8I GTNNAC 6 cut(s) 88, 571, 639, 920, 943, 1060
Hpy99I CGWCG 1 cut(s) 814
HpyAV CCTTC 5 cut(s) 466, 669, 889, 1424, 1536
HpyCH4III ACNGT 3 cut(s) 988, 1004, 1294
HpyCH4IV ACGT 3 cut(s) 236, 1298, 1306
HpyCH4V TGCA 5 cut(s) 359, 539, 1249, 1444, 1485
HpyF10VI GCNNNNNNNGC 2 cut(s) 545, 1502
HpyF3I CTNAG 4 cut(s) 381, 642, 1470, 1548
HpySE526I ACGT 3 cut(s) 236, 1298, 1306
Hsp92II CATG 5 cut(s) 325, 561, 1240, 1253, 1469
KspAI GTTAAC 3 cut(s) 88, 639, 1060
Kzo9I GATC 3 cut(s) 39, 597, 723
LmnI GCTCC 4 cut(s) 1382, 1462, 1510, 1537
Lsp1109I GCAGC 3 cut(s) 1233, 1469, 1505
LweI GCATC 1 cut(s) 1534
MaeI CTAG 6 cut(s) 153, 272, 506, 933, 1029, 1242
MaeII ACGT 3 cut(s) 236, 1298, 1306
MaeIII GTNAC 5 cut(s) 124, 482, 1207, 1294, 1302
MalI GATC 3 cut(s) 41, 599, 725
MbiI CCGCTC 1 cut(s) 1385
MboI GATC 3 cut(s) 39, 597, 723
MboII GAAGA 6 cut(s) 155, 231, 277, 1081, 1084, 1305
MfeI CAATTG 1 cut(s) 1565
MflI RGATCY 2 cut(s) 39, 597
MhlI GDGCHC 2 cut(s) 418, 1542
MlyI GAGTC 1 cut(s) 783
MmeI TCCRAC 4 cut(s) 196, 602, 795, 1158
MroXI GAANNNNTTC 1 cut(s) 1224
MseI TTAA 9 cut(s) 12, 87, 114, 296, 531, 638, 990, 1059, 1341
MspA1I CMGCKG 1 cut(s) 1561
MspI CCGG 1 cut(s) 791
MspR9I CCNGG 2 cut(s) 328, 771
MunI CAATTG 1 cut(s) 1565
MvaI CCWGG 2 cut(s) 328, 771
MwoI GCNNNNNNNGC 2 cut(s) 545, 1502
NdeII GATC 3 cut(s) 39, 597, 723
NheI GCTAGC 1 cut(s) 271
NlaIII CATG 5 cut(s) 325, 561, 1240, 1253, 1469
NlaIV GGNNCC 2 cut(s) 768, 1354
NmuCI GTSAC 2 cut(s) 1294, 1302
NspI RCATGY 2 cut(s) 1253, 1469
PaeI GCATGC 2 cut(s) 1253, 1469
PagI TCATGA 1 cut(s) 321
PdmI GAANNNNTTC 1 cut(s) 1224
PfeI GAWTC 1 cut(s) 179
PflFI GACNNNGTC 1 cut(s) 1300
PinAI ACCGGT 1 cut(s) 790
PkrI GCNGC 4 cut(s) 1248, 1484, 1495, 1563
PleI GAGTC 1 cut(s) 782
PpsI GAGTC 1 cut(s) 782
PpuMI RGGWCCY 1 cut(s) 397
PshAI GACNNNNGTC 1 cut(s) 1159
PshBI ATTAAT 1 cut(s) 1341
Psp124BI GAGCTC 1 cut(s) 1542
Psp5II RGGWCCY 1 cut(s) 397
Psp6I CCWGG 2 cut(s) 326, 769
PspFI CCCAGC 1 cut(s) 1424
PspGI CCWGG 2 cut(s) 326, 769
PspN4I GGNNCC 2 cut(s) 768, 1354
PspPI GGNCC 1 cut(s) 397
PspPPI RGGWCCY 1 cut(s) 397
PstI CTGCAG 1 cut(s) 1487
PsuI RGATCY 2 cut(s) 39, 597
PsyI GACNNNGTC 1 cut(s) 1300
RsaI GTAC 5 cut(s) 51, 436, 1291, 1334, 1557
RsaNI GTAC 5 cut(s) 50, 435, 1290, 1333, 1556
SacI GAGCTC 1 cut(s) 1542
SaqAI TTAA 9 cut(s) 12, 87, 114, 296, 531, 638, 990, 1059, 1341
SatI GCNGC 4 cut(s) 1247, 1483, 1494, 1562
Sau3AI GATC 3 cut(s) 39, 597, 723
Sau96I GGNCC 1 cut(s) 397
ScaI AGTACT 1 cut(s) 436
SchI GAGTC 1 cut(s) 783
ScrFI CCNGG 2 cut(s) 328, 771
SduI GDGCHC 2 cut(s) 418, 1542
SfaNI GCATC 1 cut(s) 1534
SfcI CTRYAG 1 cut(s) 1483
SinI GGWCC 1 cut(s) 397
SmlI CTYRAG 4 cut(s) 53, 339, 401, 1148
SmoI CTYRAG 4 cut(s) 53, 339, 401, 1148
SpeI ACTAGT 1 cut(s) 152
SphI GCATGC 2 cut(s) 1253, 1469
SsiI CCGC 3 cut(s) 300, 1385, 1561
SspI AATATT 2 cut(s) 194, 1135
SspMI CTAG 6 cut(s) 153, 272, 506, 933, 1029, 1242
SstI GAGCTC 1 cut(s) 1542
StyD4I CCNGG 2 cut(s) 326, 769
StyI CCWWGG 2 cut(s) 505, 1104
TaaI ACNGT 3 cut(s) 988, 1004, 1294
TaiI ACGT 3 cut(s) 239, 1301, 1309
TaqI TCGA 1 cut(s) 1542
TatI WGTACW 2 cut(s) 49, 434
TauI GCSGC 1 cut(s) 1564
TfiI GAWTC 1 cut(s) 179
Tru1I TTAA 9 cut(s) 12, 87, 114, 296, 531, 638, 990, 1059, 1341
Tru9I TTAA 9 cut(s) 12, 87, 114, 296, 531, 638, 990, 1059, 1341
TscAI CASTG 1 cut(s) 1056
TseFI GTSAC 2 cut(s) 1294, 1302
TseI GCWGC 3 cut(s) 1246, 1482, 1493
Tsp45I GTSAC 2 cut(s) 1294, 1302
TspDTI ATGAA 4 cut(s) 446, 908, 1100, 1253
TspGWI ACGGA 1 cut(s) 143
TspRI CASTG 1 cut(s) 1056
Tth111I GACNNNGTC 1 cut(s) 1300
VpaK11BI GGWCC 1 cut(s) 397
VspI ATTAAT 1 cut(s) 1341
XapI RAATTY 6 cut(s) 131, 385, 453, 526, 695, 980
XbaI TCTAGA 2 cut(s) 932, 1028
XceI RCATGY 2 cut(s) 1253, 1469
XmaJI CCTAGG 1 cut(s) 505
XmiI GTMKAC 1 cut(s) 919
XmnI GAANNNNTTC 1 cut(s) 1224
XspI CTAG 6 cut(s) 153, 272, 506, 933, 1029, 1242
ZrmI AGTACT 1 cut(s) 436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.