FvH4_2g26950

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000978
Physical Location & Seq
Reverse (-)
21529985 .. 21547171
17187 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g26950.t2

Sequence Viewer

Length: 1158 bp
ATGGGGTACTCTACTGGAGTAGTTATTTCTCTGTTTTGCTCATATATGCTTATATTGCTTACTACCAGTAGTACTGTGACCGCTACATTGGCTTTGAAAGACTGCGAGTTTCCAGCCATCTTCAACTTCGGAGACTCGAATTCAGATACTGGTGGACTGGCGGCAGCCTTTTTCCCATCACCCGCACCTTATGGAGAGACCTATTTCCATCTGTCACCCGGAAGATTCTCTGATGGAAGACTCATAATCGACTTCCTGGCAAAGAGTCTTGGTCACCCTTATCTAAGCGCATATCTGGATTCTCTTGGGACAAATTTCTCGTATGAGGCAAATTTCGCCACTGCTGCTTCCACTATCAGACTCCCACATCTCATTTTACCAAAAGCTGCTGGATTTAGTCCCTTCTATCTTGATATTCAATACTTGCAGTTCCTGCAACTAAAATCCAGATCACAACTCATAAGGCAACGAGGGGTAATATTTGCAAGTTTAATGCCCATGGAGGAGTACTTCTCCAAAGCTTTATACACATTCGACATTGGTCAGAATGATCTTGGGGAGGGACTCTCTCGTAACATGACTATACAGGAAGTAAATGCATCTGTTCCTGATATAATCACTGGTTTCTCAACTAATATCAAGAAAATATATGACTTGGGAGCAAGATCATTTTGGATCCACAATACGGGGCCAATTGGCTGTCTTCCTTACATTTTGGTAATATTCCCATCAGCTCAGAAAGATGAGGCTGGTTGTGCAAAGGCATGCAATGAAGTAGCTCAACATTTTAACCACAAGCTGAAGCAGGCCGTAGTTCAACTCAGGAAGGATCTTCCTTTGGCTGCATTCACTTATGTAGATGTGTATTCTGTCAAATACTCTCTTTTTAAGGAACCCCAAAAGTATGGATTTGAGCTCCCACTTGTTGCTTGTTGTGGCTATGGTGGCGAATACAACAGTAGTGCAGGATGTGGAACAACAATCTCAATCAATGGAAGCCAAATCTTTGTTGGTTCATGCAAAACCCCCTCAACTAGAGTGGTCTGGGATGGGATTCACTACACCGACGCAGCTGCGAAATTTGTTTTTAATAAAATCTCAACCGGAGCATATTCAGATCCATCCCTGTCCTTGAAGCAAGCATGTCACAGGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005488 GO:0006950 GO:0006952 GO:0008150 GO:0016787 GO:0016788 GO:0019863 GO:0019865 GO:0044877 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

386

Amino Acids

42.15

Weight (kDa)

7.53

Isoelectric Point (pI)

40.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 40 - 367 5.9e-43 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 81, 161, 183
AclWI GGATC 4 cut(s) 670, 683, 837, 1112
AcsI RAATTY 4 cut(s) 139, 313, 331, 1079
AcuI CTGAAG 1 cut(s) 821
AfaI GTAC 3 cut(s) 8, 73, 509
AfiI CCNNNNNNNGG 1 cut(s) 685
AgsI TTSAA 5 cut(s) 97, 124, 419, 818, 1135
AjnI CCWGG 1 cut(s) 255
AluBI AGCT 7 cut(s) 386, 521, 734, 779, 799, 916, 1073
AluI AGCT 7 cut(s) 386, 521, 734, 779, 799, 916, 1073
Alw21I GWGCWC 1 cut(s) 918
Alw26I GTCTC 2 cut(s) 126, 191
AlwI GGATC 4 cut(s) 670, 683, 837, 1112
AlwNI CAGNNNCTG 2 cut(s) 149, 433
AoxI GGCC 2 cut(s) 689, 807
ApeKI GCWGC 6 cut(s) 164, 344, 386, 842, 1070, 1073
ApoI RAATTY 4 cut(s) 139, 313, 331, 1079
AspLEI GCGC 1 cut(s) 290
AspS9I GGNCC 1 cut(s) 689
AsuC2I CCSGG 1 cut(s) 219
AsuHPI GGTGA 3 cut(s) 171, 207, 266
BamHI GGATCC 1 cut(s) 675
BanII GRGCYC 1 cut(s) 918
BbsI GAAGAC 2 cut(s) 244, 695
Bbv12I GWGCWC 1 cut(s) 918
BbvI GCAGC 6 cut(s) 176, 331, 373, 829, 1060, 1082
BccI CCATC 7 cut(s) 125, 184, 216, 227, 736, 1043, 1129
BceAI ACGGC 1 cut(s) 794
BcgI CGANNNNNNTGC 2 cut(s) 1055, 1089
BciT130I CCWGG 1 cut(s) 257
BcnI CCSGG 1 cut(s) 219
BcoDI GTCTC 2 cut(s) 126, 191
BfaI CTAG 1 cut(s) 1035
BisI GCNGC 7 cut(s) 162, 165, 345, 387, 843, 1071, 1074
BlsI GCNGC 7 cut(s) 163, 166, 346, 388, 844, 1072, 1075
BmcAI AGTACT 2 cut(s) 73, 509
Bme1390I CCNGG 2 cut(s) 219, 257
BmgT120I GGNCC 1 cut(s) 689
BmiI GGNNCC 3 cut(s) 677, 690, 894
BmrFI CCNGG 2 cut(s) 219, 257
BmsI GCATC 1 cut(s) 608
BoxI GACNNNNGTC 1 cut(s) 540
BpiI GAAGAC 2 cut(s) 244, 695
BplI GAGNNNNNCTC 4 cut(s) 497, 529, 551, 583
BpmI CTGGAG 1 cut(s) 36
BpuMI CCSGG 1 cut(s) 219
BsaI GGTCTC 1 cut(s) 191
BsaJI CCNNGG 1 cut(s) 498
BsaWI WCCGGW 1 cut(s) 1103
Bsc4I CCNNNNNNNGG 1 cut(s) 685
Bse1I ACTGG 5 cut(s) 19, 66, 154, 162, 625
Bse3DI GCAATG 1 cut(s) 775
BseBI CCWGG 1 cut(s) 257
BseDI CCNNGG 1 cut(s) 498
BseGI GGATG 3 cut(s) 974, 1054, 1121
BseLI CCNNNNNNNGG 1 cut(s) 685
BseMI GCAATG 1 cut(s) 775
BseMII CTCAG 2 cut(s) 749, 835
BseNI ACTGG 5 cut(s) 19, 66, 154, 162, 625
BseRI GAGGAG 1 cut(s) 518
BseXI GCAGC 6 cut(s) 176, 331, 373, 829, 1060, 1082
BsgI GTGCAG 1 cut(s) 984
BshFI GGCC 2 cut(s) 691, 809
BsiHKAI GWGCWC 1 cut(s) 918
BsiSI CCGG 2 cut(s) 219, 1104
BslFI GGGAC 3 cut(s) 322, 384, 576
BslI CCNNNNNNNGG 1 cut(s) 685
BsmAI GTCTC 2 cut(s) 126, 191
BsmFI GGGAC 3 cut(s) 322, 384, 576
BsmI GAATGC 1 cut(s) 845
BsnI GGCC 2 cut(s) 691, 809
Bso31I GGTCTC 1 cut(s) 191
Bsp1286I GDGCHC 1 cut(s) 918
Bsp143I GATC 6 cut(s) 449, 550, 665, 675, 829, 1117
Bsp19I CCATGG 1 cut(s) 498
BspACI CCGC 3 cut(s) 81, 161, 183
BspANI GGCC 2 cut(s) 691, 809
BspCNI CTCAG 2 cut(s) 748, 834
BspLI GGNNCC 3 cut(s) 677, 690, 894
BspPI GGATC 4 cut(s) 670, 683, 837, 1112
BspTNI GGTCTC 1 cut(s) 191
BsrDI GCAATG 1 cut(s) 775
BsrI ACTGG 5 cut(s) 19, 66, 154, 162, 625
BssECI CCNNGG 1 cut(s) 498
BssMI GATC 6 cut(s) 449, 550, 665, 675, 829, 1117
BssT1I CCWWGG 1 cut(s) 498
Bst2UI CCWGG 1 cut(s) 257
Bst4CI ACNGT 2 cut(s) 76, 959
BstAPI GCANNNNNTGC 1 cut(s) 433
BstC8I GCNNGC 3 cut(s) 766, 807, 1140
BstDEI CTNAG 3 cut(s) 284, 735, 821
BstDSI CCRYGG 1 cut(s) 498
BstEII GGTNACC 1 cut(s) 272
BstF5I GGATG 3 cut(s) 974, 1054, 1121
BstHHI GCGC 1 cut(s) 290
BstKTI GATC 6 cut(s) 452, 553, 668, 678, 832, 1120
BstMAI GTCTC 2 cut(s) 126, 191
BstMBI GATC 6 cut(s) 449, 550, 665, 675, 829, 1117
BstMWI GCNNNNNNNGC 7 cut(s) 55, 89, 335, 344, 433, 755, 945
BstNI CCWGG 1 cut(s) 257
BstNSI RCATGY 2 cut(s) 768, 1146
BstPAI GACNNNNGTC 1 cut(s) 540
BstPI GGTNACC 1 cut(s) 272
BstSCI CCNGG 2 cut(s) 217, 255
BstV1I GCAGC 6 cut(s) 176, 331, 373, 829, 1060, 1082
BstV2I GAAGAC 2 cut(s) 244, 695
BstX2I RGATCY 3 cut(s) 675, 829, 1117
BstXI CCANNNNNNTGG 1 cut(s) 905
BstYI RGATCY 3 cut(s) 675, 829, 1117
BsuRI GGCC 2 cut(s) 691, 809
BtgI CCRYGG 1 cut(s) 498
BtsCI GGATG 3 cut(s) 974, 1054, 1121
BtsI GCAGTG 1 cut(s) 339
BtsIMutI CAGTG 2 cut(s) 339, 618
Cac8I GCNNGC 3 cut(s) 766, 807, 1140
CaiI CAGNNNCTG 2 cut(s) 149, 433
CfoI GCGC 1 cut(s) 290
Cfr13I GGNCC 1 cut(s) 689
CseI GACGC 1 cut(s) 1076
Csp6I GTAC 3 cut(s) 7, 72, 508
CspCI CAANNNNNGTGG 2 cut(s) 1020, 1055
CviAII CATG 5 cut(s) 499, 577, 765, 1017, 1143
CviQI GTAC 3 cut(s) 7, 72, 508
DdeI CTNAG 3 cut(s) 284, 735, 821
DpnI GATC 6 cut(s) 451, 552, 667, 677, 831, 1119
DpnII GATC 6 cut(s) 449, 550, 665, 675, 829, 1117
Ecl136II GAGCTC 1 cut(s) 916
Eco130I CCWWGG 1 cut(s) 498
Eco24I GRGCYC 1 cut(s) 918
Eco31I GGTCTC 1 cut(s) 191
Eco53kI GAGCTC 1 cut(s) 916
Eco57I CTGAAG 1 cut(s) 821
Eco91I GGTNACC 1 cut(s) 272
EcoICRI GAGCTC 1 cut(s) 916
EcoO65I GGTNACC 1 cut(s) 272
EcoRI GAATTC 1 cut(s) 139
EcoRII CCWGG 1 cut(s) 255
EcoT14I CCWWGG 1 cut(s) 498
EcoT22I ATGCAT 1 cut(s) 601
EcoT38I GRGCYC 1 cut(s) 918
ErhI CCWWGG 1 cut(s) 498
FaeI CATG 5 cut(s) 502, 580, 768, 1020, 1146
FaqI GGGAC 3 cut(s) 322, 384, 576
FatI CATG 5 cut(s) 498, 576, 764, 1016, 1142
FauI CCCGC 1 cut(s) 190
Fnu4HI GCNGC 7 cut(s) 162, 165, 345, 387, 843, 1071, 1074
FokI GGATG 3 cut(s) 981, 1061, 1108
FriOI GRGCYC 1 cut(s) 918
Fsp4HI GCNGC 7 cut(s) 162, 165, 345, 387, 843, 1071, 1074
FspBI CTAG 1 cut(s) 1035
GlaI GCGC 1 cut(s) 289
GluI GCNGC 7 cut(s) 162, 165, 345, 387, 843, 1071, 1074
GsuI CTGGAG 1 cut(s) 36
HaeIII GGCC 2 cut(s) 691, 809
HapII CCGG 2 cut(s) 219, 1104
HgaI GACGC 1 cut(s) 1076
HhaI GCGC 1 cut(s) 290
Hin1II CATG 5 cut(s) 502, 580, 768, 1020, 1146
Hin6I GCGC 1 cut(s) 288
HinP1I GCGC 1 cut(s) 288
HindIII AAGCTT 1 cut(s) 519
HinfI GANTC 8 cut(s) 134, 225, 240, 265, 299, 360, 564, 1054
HpaII CCGG 2 cut(s) 219, 1104
HphI GGTGA 3 cut(s) 171, 207, 266
Hpy166II GTNNAC 1 cut(s) 155
Hpy188I TCNGA 7 cut(s) 131, 145, 232, 359, 546, 738, 1117
Hpy188III TCNNGA 6 cut(s) 296, 410, 447, 608, 640, 823
Hpy8I GTNNAC 1 cut(s) 155
Hpy99I CGWCG 1 cut(s) 1070
HpyAV CCTTC 2 cut(s) 412, 820
HpyCH4III ACNGT 2 cut(s) 76, 959
HpyCH4V TGCA 9 cut(s) 427, 436, 485, 599, 758, 768, 845, 965, 1020
HpyF10VI GCNNNNNNNGC 7 cut(s) 55, 89, 335, 344, 433, 755, 945
HpyF3I CTNAG 3 cut(s) 284, 735, 821
Hsp92II CATG 5 cut(s) 502, 580, 768, 1020, 1146
HspAI GCGC 1 cut(s) 288
Kzo9I GATC 6 cut(s) 449, 550, 665, 675, 829, 1117
LmnI GCTCC 3 cut(s) 659, 921, 1106
Lsp1109I GCAGC 6 cut(s) 176, 331, 373, 829, 1060, 1082
LweI GCATC 1 cut(s) 608
MaeI CTAG 1 cut(s) 1035
MaeIII GTNAC 5 cut(s) 76, 213, 272, 572, 1145
MalI GATC 6 cut(s) 451, 552, 667, 677, 831, 1119
MboI GATC 6 cut(s) 449, 550, 665, 675, 829, 1117
MboII GAAGA 5 cut(s) 112, 234, 249, 695, 824
MfeI CAATTG 1 cut(s) 693
MflI RGATCY 3 cut(s) 675, 829, 1117
MhlI GDGCHC 1 cut(s) 918
MluCI AATT 5 cut(s) 139, 313, 331, 693, 1079
MlyI GAGTC 5 cut(s) 128, 234, 274, 354, 558
MnlI CCTC 6 cut(s) 319, 464, 496, 553, 739, 1039
Mph1103I ATGCAT 1 cut(s) 601
MseI TTAA 4 cut(s) 491, 789, 888, 1089
MspA1I CMGCKG 1 cut(s) 1073
MspI CCGG 2 cut(s) 219, 1104
MspR9I CCNGG 2 cut(s) 219, 257
MunI CAATTG 1 cut(s) 693
Mva1269I GAATGC 1 cut(s) 845
MvaI CCWGG 1 cut(s) 257
MwoI GCNNNNNNNGC 7 cut(s) 55, 89, 335, 344, 433, 755, 945
NciI CCSGG 1 cut(s) 219
NcoI CCATGG 1 cut(s) 498
NdeII GATC 6 cut(s) 449, 550, 665, 675, 829, 1117
NlaIII CATG 5 cut(s) 502, 580, 768, 1020, 1146
NlaIV GGNNCC 3 cut(s) 677, 690, 894
NmuCI GTSAC 4 cut(s) 76, 213, 272, 1145
NsiI ATGCAT 1 cut(s) 601
NspI RCATGY 2 cut(s) 768, 1146
PaeI GCATGC 1 cut(s) 768
PctI GAATGC 1 cut(s) 845
PfeI GAWTC 3 cut(s) 225, 299, 1054
PkrI GCNGC 7 cut(s) 163, 166, 346, 388, 844, 1072, 1075
PleI GAGTC 5 cut(s) 128, 234, 273, 354, 558
PpsI GAGTC 5 cut(s) 128, 234, 273, 354, 558
PshAI GACNNNNGTC 1 cut(s) 540
Psp124BI GAGCTC 1 cut(s) 918
Psp6I CCWGG 1 cut(s) 255
PspEI GGTNACC 1 cut(s) 272
PspGI CCWGG 1 cut(s) 255
PspN4I GGNNCC 3 cut(s) 677, 690, 894
PspPI GGNCC 1 cut(s) 689
PstNI CAGNNNCTG 2 cut(s) 149, 433
PsuI RGATCY 3 cut(s) 675, 829, 1117
PvuII CAGCTG 1 cut(s) 1073
RsaI GTAC 3 cut(s) 8, 73, 509
RsaNI GTAC 3 cut(s) 7, 72, 508
SacI GAGCTC 1 cut(s) 918
SaqAI TTAA 4 cut(s) 491, 789, 888, 1089
SatI GCNGC 7 cut(s) 162, 165, 345, 387, 843, 1071, 1074
Sau3AI GATC 6 cut(s) 449, 550, 665, 675, 829, 1117
Sau96I GGNCC 1 cut(s) 689
ScaI AGTACT 2 cut(s) 73, 509
SchI GAGTC 5 cut(s) 128, 234, 274, 354, 558
ScrFI CCNGG 2 cut(s) 219, 257
SduI GDGCHC 1 cut(s) 918
SetI ASST 9 cut(s) 190, 203, 388, 523, 736, 781, 801, 918, 1075
SfaNI GCATC 1 cut(s) 608
SphI GCATGC 1 cut(s) 768
Sse9I AATT 5 cut(s) 139, 313, 331, 693, 1079
SsiI CCGC 3 cut(s) 81, 161, 183
SspI AATATT 2 cut(s) 480, 723
SspMI CTAG 1 cut(s) 1035
SstI GAGCTC 1 cut(s) 918
StyD4I CCNGG 2 cut(s) 217, 255
StyI CCWWGG 1 cut(s) 498
TaaI ACNGT 2 cut(s) 76, 959
TaqI TCGA 3 cut(s) 137, 249, 534
TasI AATT 5 cut(s) 139, 313, 331, 693, 1079
TatI WGTACW 2 cut(s) 71, 507
TauI GCSGC 1 cut(s) 164
TfiI GAWTC 3 cut(s) 225, 299, 1054
Tru1I TTAA 4 cut(s) 491, 789, 888, 1089
Tru9I TTAA 4 cut(s) 491, 789, 888, 1089
TscAI CASTG 2 cut(s) 346, 625
TseFI GTSAC 4 cut(s) 76, 213, 272, 1145
TseI GCWGC 6 cut(s) 164, 344, 386, 842, 1070, 1073
Tsp45I GTSAC 4 cut(s) 76, 213, 272, 1145
TspDTI ATGAA 2 cut(s) 786, 1005
TspRI CASTG 2 cut(s) 346, 625
XapI RAATTY 4 cut(s) 139, 313, 331, 1079
XceI RCATGY 2 cut(s) 768, 1146
XcmI CCANNNNNNNNNTGG 1 cut(s) 1007
XspI CTAG 1 cut(s) 1035
ZrmI AGTACT 2 cut(s) 73, 509
Zsp2I ATGCAT 1 cut(s) 601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.