Rmu_sc0007986.1_g000005

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0007986.1
OG0000978
Physical Location & Seq
Reverse (-)
33312 .. 36040
2729 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0007986.1_g000005.1.cds

Sequence Viewer

Length: 1386 bp
atggagtacactcctagagttcttatttctctgttctgctgctatatgctcctattgcatattaccagtattactgtaagccctgccttggctttggaagactgcgagtttccagccatcttcaacttcggagactcaaattcggatactggtggattcgcagcatccatttatcccccacctacaccttatggagagacctattttcatatgccggttggaagaatctcagatggaagactcattgtcgatttcctcgcaaagagtcttggtcatccttatctaagcgcatatctagattccctcgggaccaatttctcgcatggtgcaaattttgcaacttcagcttccactatcagactcccagatctcattttaccagccggcggatttagtcccttctaccttgatattcaatacgtacagttccgggaactaaagtccagatcacaactcataaggcaacgagcaaagaggcttggtcatccttatctaagcgcatatctagattccctcgggaccaatttctcgcatggtgcaaattttgcaacttcagcttccactatcagactcccagatctcattttaccagccggcggatttagtcccttctaccttgatattcaatacgtacagttccgggaactaaagtccagatcacaactcataaggcaacgagggggaatatttgcaagtttaatgcccaaggaggagtacttctccaaagctttatacacattcgacattggtcagaatgatcttggcgagggatttttcggtaacaagactatacaggaagtcaatgcatctgttcctgatataatcactggtttctcaacgaatatcaagaaaatatatgatttgggggcaagatcattttggatccacaatacggggccgattggctgtgtcccttacattttggtgaaattcccatcggctcagaaagatgaggttggctgtgcaaaggcttacaatgaagtagctcaacattttaaccacgagctgaagcaggccatagttcaactcaggaaggatcttcctttggctgcattcacttatgtagatgtgtattccgtcaaatattctattttcaaggaacccaaaaaatacggatttgagctcccacttgttgcttgttgtggctatggtggtaagtacaactataacagtagtacaggatgtggagcaacaatcacagtcaatggaagtcaaatctttgttggctcatgcaagaacccatcagctagagtgaactgggatgggattcattatactgaggcagctgcgaaatttgtttttgagacaatctcaactggagcatattcagatccacctctgccattgaaacaagcatgccacaggagtcgaaataagaaaaaataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005488 GO:0006950 GO:0006952 GO:0008150 GO:0016787 GO:0016788 GO:0019863 GO:0019865 GO:0044877 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

461

Amino Acids

51.1

Weight (kDa)

8.72

Isoelectric Point (pI)

38.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Style Settings
Image
Tree File
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 387, 597
AclWI GGATC 4 cut(s) 877, 890, 1044, 1325
AcsI RAATTY 5 cut(s) 139, 331, 541, 929, 1292
AcuI CTGAAG 3 cut(s) 327, 537, 1028
AfaI GTAC 6 cut(s) 8, 423, 633, 716, 1160, 1177
AfiI CCNNNNNNNGG 4 cut(s) 88, 386, 596, 892
AgsI TTSAA 6 cut(s) 124, 416, 626, 1025, 1096, 1348
AhdI GACNNNNNGTC 1 cut(s) 245
AluBI AGCT 8 cut(s) 347, 557, 728, 986, 1006, 1123, 1247, 1286
AluI AGCT 8 cut(s) 347, 557, 728, 986, 1006, 1123, 1247, 1286
Alw21I GWGCWC 1 cut(s) 1125
Alw26I GTCTC 3 cut(s) 126, 191, 1298
AlwI GGATC 4 cut(s) 877, 890, 1044, 1325
Ama87I CYCGRG 2 cut(s) 305, 515
AoxI GGCC 2 cut(s) 896, 1014
ApeKI GCWGC 5 cut(s) 39, 161, 1049, 1283, 1286
ApoI RAATTY 5 cut(s) 139, 331, 541, 929, 1292
AspLEI GCGC 2 cut(s) 290, 500
AspS9I GGNCC 3 cut(s) 309, 519, 896
AsuC2I CCSGG 2 cut(s) 431, 641
AsuHPI GGTGA 1 cut(s) 937
AvaI CYCGRG 2 cut(s) 305, 515
AvaII GGWCC 2 cut(s) 309, 519
BamHI GGATCC 1 cut(s) 882
BanII GRGCYC 1 cut(s) 1125
BauI CACGAG 1 cut(s) 1001
BbsI GAAGAC 2 cut(s) 105, 244
Bbv12I GWGCWC 1 cut(s) 1125
BbvI GCAGC 5 cut(s) 26, 173, 1036, 1273, 1295
BccI CCATC 5 cut(s) 125, 227, 943, 1249, 1256
BciVI GTATCC 1 cut(s) 139
BcnI CCSGG 2 cut(s) 431, 641
BcoDI GTCTC 3 cut(s) 126, 191, 1298
BfaI CTAG 4 cut(s) 15, 296, 506, 1248
BfuI GTATCC 1 cut(s) 139
BglII AGATCT 2 cut(s) 367, 577
BisI GCNGC 5 cut(s) 40, 162, 1050, 1284, 1287
BlsI GCNGC 5 cut(s) 41, 163, 1051, 1285, 1288
BmcAI AGTACT 1 cut(s) 716
Bme1390I CCNGG 2 cut(s) 431, 641
Bme18I GGWCC 2 cut(s) 309, 519
BmeRI GACNNNNNGTC 1 cut(s) 245
BmeT110I CYCGRG 2 cut(s) 305, 515
BmgT120I GGNCC 3 cut(s) 309, 519, 896
BmiI GGNNCC 5 cut(s) 310, 520, 884, 897, 1101
BmrFI CCNGG 2 cut(s) 431, 641
BmrI ACTGGG 1 cut(s) 1267
BmsI GCATC 2 cut(s) 173, 815
BmuI ACTGGG 1 cut(s) 1267
BoxI GACNNNNGTC 1 cut(s) 747
BpiI GAAGAC 2 cut(s) 105, 244
BplI GAGNNNNNCTC 4 cut(s) 704, 736, 1295, 1327
BpmI CTGGAG 1 cut(s) 1338
BpuMI CCSGG 2 cut(s) 431, 641
BsaAI YACGTR 2 cut(s) 421, 631
BsaI GGTCTC 1 cut(s) 191
BsaJI CCNNGG 4 cut(s) 87, 304, 514, 705
Bsc4I CCNNNNNNNGG 4 cut(s) 88, 386, 596, 892
Bse118I RCCGGY 3 cut(s) 214, 383, 593
Bse1I ACTGG 5 cut(s) 66, 154, 832, 1262, 1321
BseDI CCNNGG 4 cut(s) 87, 304, 514, 705
BseGI GGATG 5 cut(s) 164, 274, 484, 1187, 1267
BseLI CCNNNNNNNGG 4 cut(s) 88, 386, 596, 892
BseMII CTCAG 4 cut(s) 243, 956, 1042, 1269
BseNI ACTGG 5 cut(s) 66, 154, 832, 1262, 1321
BseRI GAGGAG 1 cut(s) 725
BseXI GCAGC 5 cut(s) 26, 173, 1036, 1273, 1295
BshFI GGCC 2 cut(s) 898, 1016
BsiHKAI GWGCWC 1 cut(s) 1125
BsiHKCI CYCGRG 2 cut(s) 305, 515
BsiSI CCGG 5 cut(s) 215, 384, 430, 594, 640
BslFI GGGAC 5 cut(s) 322, 381, 532, 591, 896
BslI CCNNNNNNNGG 4 cut(s) 88, 386, 596, 892
BsmAI GTCTC 3 cut(s) 126, 191, 1298
BsmFI GGGAC 5 cut(s) 322, 381, 532, 591, 896
BsmI GAATGC 1 cut(s) 1052
BsnI GGCC 2 cut(s) 898, 1016
Bso31I GGTCTC 1 cut(s) 191
BsoBI CYCGRG 2 cut(s) 305, 515
Bsp1286I GDGCHC 1 cut(s) 1125
Bsp143I GATC 9 cut(s) 367, 446, 577, 656, 757, 872, 882, 1036, 1330
BspACI CCGC 2 cut(s) 387, 597
BspANI GGCC 2 cut(s) 898, 1016
BspCNI CTCAG 4 cut(s) 242, 955, 1041, 1270
BspLI GGNNCC 5 cut(s) 310, 520, 884, 897, 1101
BspPI GGATC 4 cut(s) 877, 890, 1044, 1325
BspTNI GGTCTC 1 cut(s) 191
BsrFI RCCGGY 3 cut(s) 214, 383, 593
BsrI ACTGG 5 cut(s) 66, 154, 832, 1262, 1321
BssAI RCCGGY 3 cut(s) 214, 383, 593
BssECI CCNNGG 4 cut(s) 87, 304, 514, 705
BssMI GATC 9 cut(s) 367, 446, 577, 656, 757, 872, 882, 1036, 1330
BssSI CACGAG 1 cut(s) 1001
BssT1I CCWWGG 2 cut(s) 87, 705
Bst2BI CACGAG 1 cut(s) 1001
Bst4CI ACNGT 5 cut(s) 76, 426, 636, 1172, 1201
BstAPI GCANNNNNTGC 2 cut(s) 335, 545
BstBAI YACGTR 2 cut(s) 421, 631
BstC8I GCNNGC 4 cut(s) 385, 595, 1014, 1357
BstDEI CTNAG 6 cut(s) 229, 284, 494, 942, 1028, 1278
BstF5I GGATG 5 cut(s) 164, 274, 484, 1187, 1267
BstHHI GCGC 2 cut(s) 290, 500
BstKTI GATC 9 cut(s) 370, 449, 580, 659, 760, 875, 885, 1039, 1333
BstMAI GTCTC 3 cut(s) 126, 191, 1298
BstMBI GATC 9 cut(s) 367, 446, 577, 656, 757, 872, 882, 1036, 1330
BstMWI GCNNNNNNNGC 5 cut(s) 55, 335, 344, 545, 554
BstNSI RCATGY 1 cut(s) 1359
BstPAI GACNNNNGTC 1 cut(s) 747
BstSCI CCNGG 2 cut(s) 429, 639
BstSNI TACGTA 2 cut(s) 421, 631
BstV1I GCAGC 5 cut(s) 26, 173, 1036, 1273, 1295
BstV2I GAAGAC 2 cut(s) 105, 244
BstX2I RGATCY 5 cut(s) 367, 577, 882, 1036, 1330
BstYI RGATCY 5 cut(s) 367, 577, 882, 1036, 1330
BsuI GTATCC 1 cut(s) 139
BsuRI GGCC 2 cut(s) 898, 1016
BtsCI GGATG 5 cut(s) 164, 274, 484, 1187, 1267
BtsIMutI CAGTG 1 cut(s) 825
Cac8I GCNNGC 4 cut(s) 385, 595, 1014, 1357
CfoI GCGC 2 cut(s) 290, 500
Cfr10I RCCGGY 3 cut(s) 214, 383, 593
Cfr13I GGNCC 3 cut(s) 309, 519, 896
Csp6I GTAC 6 cut(s) 7, 422, 632, 715, 1159, 1176
CviAII CATG 4 cut(s) 323, 533, 1230, 1356
CviQI GTAC 6 cut(s) 7, 422, 632, 715, 1159, 1176
DdeI CTNAG 6 cut(s) 229, 284, 494, 942, 1028, 1278
DpnI GATC 9 cut(s) 369, 448, 579, 658, 759, 874, 884, 1038, 1332
DpnII GATC 9 cut(s) 367, 446, 577, 656, 757, 872, 882, 1036, 1330
DriI GACNNNNNGTC 1 cut(s) 245
Eam1105I GACNNNNNGTC 1 cut(s) 245
EciI GGCGGA 2 cut(s) 402, 612
Ecl136II GAGCTC 1 cut(s) 1123
Eco105I TACGTA 2 cut(s) 421, 631
Eco130I CCWWGG 2 cut(s) 87, 705
Eco24I GRGCYC 1 cut(s) 1125
Eco31I GGTCTC 1 cut(s) 191
Eco47I GGWCC 2 cut(s) 309, 519
Eco53kI GAGCTC 1 cut(s) 1123
Eco57I CTGAAG 3 cut(s) 327, 537, 1028
Eco88I CYCGRG 2 cut(s) 305, 515
EcoICRI GAGCTC 1 cut(s) 1123
EcoT14I CCWWGG 2 cut(s) 87, 705
EcoT22I ATGCAT 1 cut(s) 808
EcoT38I GRGCYC 1 cut(s) 1125
ErhI CCWWGG 2 cut(s) 87, 705
FaeI CATG 4 cut(s) 326, 536, 1233, 1359
FaqI GGGAC 5 cut(s) 322, 381, 532, 591, 896
FatI CATG 4 cut(s) 322, 532, 1229, 1355
FauNDI CATATG 1 cut(s) 210
Fnu4HI GCNGC 5 cut(s) 40, 162, 1050, 1284, 1287
FokI GGATG 5 cut(s) 151, 261, 471, 1194, 1274
FriOI GRGCYC 1 cut(s) 1125
Fsp4HI GCNGC 5 cut(s) 40, 162, 1050, 1284, 1287
FspBI CTAG 4 cut(s) 15, 296, 506, 1248
GlaI GCGC 2 cut(s) 289, 499
GluI GCNGC 5 cut(s) 40, 162, 1050, 1284, 1287
GsuI CTGGAG 1 cut(s) 1338
HaeIII GGCC 2 cut(s) 898, 1016
HapII CCGG 5 cut(s) 215, 384, 430, 594, 640
HhaI GCGC 2 cut(s) 290, 500
Hin1II CATG 4 cut(s) 326, 536, 1233, 1359
Hin6I GCGC 2 cut(s) 288, 498
HinP1I GCGC 2 cut(s) 288, 498
HindIII AAGCTT 1 cut(s) 726
HpaII CCGG 5 cut(s) 215, 384, 430, 594, 640
HphI GGTGA 1 cut(s) 937
Hpy166II GTNNAC 2 cut(s) 9, 1255
Hpy188I TCNGA 8 cut(s) 131, 145, 232, 359, 569, 753, 945, 1330
Hpy188III TCNNGA 9 cut(s) 296, 307, 444, 506, 517, 654, 815, 847, 1030
Hpy8I GTNNAC 2 cut(s) 9, 1255
HpyAV CCTTC 3 cut(s) 409, 619, 1027
HpyCH4III ACNGT 5 cut(s) 76, 426, 636, 1172, 1201
HpyCH4IV ACGT 2 cut(s) 420, 630
HpyF10VI GCNNNNNNNGC 5 cut(s) 55, 335, 344, 545, 554
HpyF3I CTNAG 6 cut(s) 229, 284, 494, 942, 1028, 1278
HpySE526I ACGT 2 cut(s) 420, 630
Hsp92II CATG 4 cut(s) 326, 536, 1233, 1359
HspAI GCGC 2 cut(s) 288, 498
KroI GCCGGC 2 cut(s) 383, 593
KroNI GCCGGC 2 cut(s) 385, 595
Kzo9I GATC 9 cut(s) 367, 446, 577, 656, 757, 872, 882, 1036, 1330
LmnI GCTCC 4 cut(s) 54, 1128, 1187, 1319
Lsp1109I GCAGC 5 cut(s) 26, 173, 1036, 1273, 1295
LweI GCATC 2 cut(s) 173, 815
MaeI CTAG 4 cut(s) 15, 296, 506, 1248
MaeII ACGT 2 cut(s) 420, 630
MaeIII GTNAC 1 cut(s) 779
MalI GATC 9 cut(s) 369, 448, 579, 658, 759, 874, 884, 1038, 1332
MboI GATC 9 cut(s) 367, 446, 577, 656, 757, 872, 882, 1036, 1330
MboII GAAGA 5 cut(s) 110, 112, 234, 249, 1031
MflI RGATCY 5 cut(s) 367, 577, 882, 1036, 1330
MhlI GDGCHC 1 cut(s) 1125
MluCI AATT 7 cut(s) 139, 313, 331, 523, 541, 929, 1292
MlyI GAGTC 6 cut(s) 128, 234, 274, 354, 564, 1375
MmeI TCCRAC 1 cut(s) 199
Mph1103I ATGCAT 1 cut(s) 808
MroNI GCCGGC 2 cut(s) 383, 593
MseI TTAA 2 cut(s) 698, 996
MslI CAYNNNNRTG 1 cut(s) 923
MspA1I CMGCKG 1 cut(s) 1286
MspI CCGG 5 cut(s) 215, 384, 430, 594, 640
MspR9I CCNGG 2 cut(s) 431, 641
Mva1269I GAATGC 1 cut(s) 1052
MwoI GCNNNNNNNGC 5 cut(s) 55, 335, 344, 545, 554
NaeI GCCGGC 2 cut(s) 385, 595
NciI CCSGG 2 cut(s) 431, 641
NdeI CATATG 1 cut(s) 210
NdeII GATC 9 cut(s) 367, 446, 577, 656, 757, 872, 882, 1036, 1330
NgoMIV GCCGGC 2 cut(s) 383, 593
NlaIII CATG 4 cut(s) 326, 536, 1233, 1359
NlaIV GGNNCC 5 cut(s) 310, 520, 884, 897, 1101
NsiI ATGCAT 1 cut(s) 808
NspI RCATGY 1 cut(s) 1359
PaeI GCATGC 1 cut(s) 1359
PctI GAATGC 1 cut(s) 1052
PdiI GCCGGC 2 cut(s) 385, 595
PfeI GAWTC 5 cut(s) 156, 225, 299, 509, 1267
PfoI TCCNGGA 2 cut(s) 429, 639
PkrI GCNGC 5 cut(s) 41, 163, 1051, 1285, 1288
PleI GAGTC 6 cut(s) 128, 234, 273, 354, 564, 1374
PpsI GAGTC 6 cut(s) 128, 234, 273, 354, 564, 1374
Ppu21I YACGTR 2 cut(s) 421, 631
PshAI GACNNNNGTC 1 cut(s) 747
Psp124BI GAGCTC 1 cut(s) 1125
PspN4I GGNNCC 5 cut(s) 310, 520, 884, 897, 1101
PspPI GGNCC 3 cut(s) 309, 519, 896
PsuI RGATCY 5 cut(s) 367, 577, 882, 1036, 1330
PvuII CAGCTG 1 cut(s) 1286
RsaI GTAC 6 cut(s) 8, 423, 633, 716, 1160, 1177
RsaNI GTAC 6 cut(s) 7, 422, 632, 715, 1159, 1176
RseI CAYNNNNRTG 1 cut(s) 923
SacI GAGCTC 1 cut(s) 1125
SaqAI TTAA 2 cut(s) 698, 996
SatI GCNGC 5 cut(s) 40, 162, 1050, 1284, 1287
Sau3AI GATC 9 cut(s) 367, 446, 577, 656, 757, 872, 882, 1036, 1330
Sau96I GGNCC 3 cut(s) 309, 519, 896
ScaI AGTACT 1 cut(s) 716
SchI GAGTC 6 cut(s) 128, 234, 274, 354, 564, 1375
ScrFI CCNGG 2 cut(s) 431, 641
SduI GDGCHC 1 cut(s) 1125
SfaNI GCATC 2 cut(s) 173, 815
SinI GGWCC 2 cut(s) 309, 519
SmiMI CAYNNNNRTG 1 cut(s) 923
SnaBI TACGTA 2 cut(s) 421, 631
SphI GCATGC 1 cut(s) 1359
Sse9I AATT 7 cut(s) 139, 313, 331, 523, 541, 929, 1292
SsiI CCGC 2 cut(s) 387, 597
SspI AATATT 2 cut(s) 687, 1085
SspMI CTAG 4 cut(s) 15, 296, 506, 1248
SstI GAGCTC 1 cut(s) 1125
StyD4I CCNGG 2 cut(s) 429, 639
StyI CCWWGG 2 cut(s) 87, 705
TaaI ACNGT 5 cut(s) 76, 426, 636, 1172, 1201
TaiI ACGT 2 cut(s) 423, 633
TaqI TCGA 3 cut(s) 249, 741, 1369
TasI AATT 7 cut(s) 139, 313, 331, 523, 541, 929, 1292
TatI WGTACW 4 cut(s) 6, 714, 1158, 1175
TfiI GAWTC 5 cut(s) 156, 225, 299, 509, 1267
Tru1I TTAA 2 cut(s) 698, 996
Tru9I TTAA 2 cut(s) 698, 996
TscAI CASTG 1 cut(s) 832
TseI GCWGC 5 cut(s) 39, 161, 1049, 1283, 1286
TspDTI ATGAA 3 cut(s) 197, 993, 1259
TspGWI ACGGA 2 cut(s) 1066, 1128
TspRI CASTG 1 cut(s) 832
VpaK11BI GGWCC 2 cut(s) 309, 519
XapI RAATTY 5 cut(s) 139, 331, 541, 929, 1292
XbaI TCTAGA 2 cut(s) 295, 505
XceI RCATGY 1 cut(s) 1359
XspI CTAG 4 cut(s) 15, 296, 506, 1248
ZrmI AGTACT 1 cut(s) 716
Zsp2I ATGCAT 1 cut(s) 808
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.