MD15G1348800.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000978
Physical Location & Seq
Forward (+)
41327111 .. 41332181
5071 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1348800.v1.1.491

Sequence Viewer

Length: 1173 bp
ATGGAGTCACCGATAATTACTATCAAAGTTATCATTTCATTCTTTTGCTTGTACATGCTTTTGTCGCACTTGCATCTCACCACTTTGAACCCTACTGCCTTTGCTTTGGAGGACTGTCAGTTTCCAACCGTCTTTAACTTTGGGGACTCTAATTCAGATACTGGCGGATTGGCCTCATCCCTTATTGCACCAACCCCTCCTTATGGTGAGACATTTTTTGGTATGCCGGCCGGAAGATTCGCAGATGGCCGTCTCACAATCGATTTCCTCGCAAAAAGTGTTGGCCACCCTTTTCTAAGCGCATATTTGGATTCACTTGGGACCAACTTCTCGTCCGGTGCAAATTTTGCCACTGCAGCTTCCACAATCAGGCTGCCCAATAGCGTTATACCAAGTGGTGGATTTAGCCCTTTCTTCCTTGATATTCAGTACATGCAGTTCATGCAATTCAAATCCAGATCACGACTCATAAGGCAAAGAGGAGGAATATATGCAAGTTTACTGCCCAAGGATGAGTACTTCCCCAAAGCTTTATACACATTTGACATTGGTCAGAACGACCTTGGGGAAGGGTTCTTTGCAAACAAGACTATAAAGGAGGTGAATGCATCTGTTCCTGATATAATCATCAAGTTCTCAACAAATATTCAGAAAATATATGACTTGGGAGCCAGATTGTTTTGGATCCACAACACTGGGCCAATTGGCTGTCTCCCTTACATTTTAGCTAATTTTCCATCAGCTCAAAAGGACGAGGTTGGCTGCGCAAAGTCTTACAATGAAGTGGCTCAGTATTTTAACCACCAACTGAAGGAAGCCACAGTTCAACTTAGGAAGGATCTTCCTTTGGCTGCATTTACTTACGTGGACATATATTCTGTCAAATACTCTCTTTACAAGGAACCAAAAAAATATGGGTTTGAGTTCCCACTTGTTGCTTGTTGTGGGTTTGGTGGGAAGCACAACTATAGCAGTAGTGCTGGATGTGGAACAACAGTTACAGTCAATGGAAGCCAAATATTTGTTGGTTCGTGCAAAAGCCCGTCAGTGAGAGTGAATTGGGATGGCATTCATTATACTGAGGCAGCTAACAAGTTTGTTTTTGACAAAATTTCAACTGGAGCATTTTCAGATCCACCCCTTCCCTTAAAACATGCATGTCACAGGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005488 GO:0006950 GO:0006952 GO:0008150 GO:0016787 GO:0016788 GO:0019863 GO:0019865 GO:0044877 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

391

Amino Acids

42.99

Weight (kDa)

8.26

Isoelectric Point (pI)

35.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 44 - 371 8.7e-47 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 766
AccB7I CCANNNNNTGG 1 cut(s) 398
AciI CCGC 1 cut(s) 165
AclWI GGATC 4 cut(s) 679, 692, 846, 1127
AcoI YGGCCR 3 cut(s) 228, 247, 283
AcsI RAATTY 2 cut(s) 343, 1110
AcuI CTGAAG 1 cut(s) 830
AfaI GTAC 3 cut(s) 53, 431, 518
AfiI CCNNNNNNNGG 4 cut(s) 203, 369, 398, 811
AgsI TTSAA 4 cut(s) 88, 451, 827, 1116
AluBI AGCT 5 cut(s) 359, 530, 728, 743, 1088
AluI AGCT 5 cut(s) 359, 530, 728, 743, 1088
Alw26I GTCTC 3 cut(s) 203, 257, 716
AlwI GGATC 4 cut(s) 679, 692, 846, 1127
AlwNI CAGNNNCTG 1 cut(s) 161
AoxI GGCC 5 cut(s) 171, 228, 247, 283, 698
ApeKI GCWGC 5 cut(s) 356, 373, 762, 851, 1085
ApoI RAATTY 2 cut(s) 343, 1110
AspLEI GCGC 2 cut(s) 302, 767
AspS9I GGNCC 2 cut(s) 321, 698
AsuHPI GGTGA 3 cut(s) 70, 218, 613
AvaII GGWCC 1 cut(s) 321
BalI TGGCCA 1 cut(s) 285
BamHI GGATCC 1 cut(s) 684
BbvI GCAGC 5 cut(s) 360, 368, 749, 838, 1097
BccI CCATC 3 cut(s) 239, 745, 1058
BceAI ACGGC 1 cut(s) 234
BcoDI GTCTC 3 cut(s) 203, 257, 716
BfmI CTRYAG 2 cut(s) 354, 967
BisI GCNGC 5 cut(s) 357, 374, 763, 852, 1086
BlsI GCNGC 5 cut(s) 358, 375, 764, 853, 1087
BmcAI AGTACT 1 cut(s) 518
Bme18I GGWCC 1 cut(s) 321
BmgT120I GGNCC 2 cut(s) 321, 698
BmiI GGNNCC 4 cut(s) 322, 670, 686, 903
BmrI ACTGGG 1 cut(s) 705
BmsI GCATC 2 cut(s) 82, 617
BmuI ACTGGG 1 cut(s) 705
BoxI GACNNNNGTC 1 cut(s) 549
BpmI CTGGAG 1 cut(s) 1140
Bsa29I ATCGAT 1 cut(s) 261
BsaAI YACGTR 1 cut(s) 865
BsaJI CCNNGG 2 cut(s) 507, 562
BsaWI WCCGGW 1 cut(s) 335
Bsc4I CCNNNNNNNGG 4 cut(s) 203, 369, 398, 811
Bse118I RCCGGY 1 cut(s) 226
Bse1I ACTGG 3 cut(s) 166, 700, 1123
BseCI ATCGAT 1 cut(s) 261
BseDI CCNNGG 2 cut(s) 507, 562
BseGI GGATG 4 cut(s) 176, 517, 989, 1069
BseLI CCNNNNNNNGG 4 cut(s) 203, 369, 398, 811
BseMII CTCAG 2 cut(s) 803, 1071
BseNI ACTGG 3 cut(s) 166, 700, 1123
BseRI GAGGAG 1 cut(s) 495
BseX3I CGGCCG 1 cut(s) 228
BseXI GCAGC 5 cut(s) 360, 368, 749, 838, 1097
Bsh1285I CGRYCG 1 cut(s) 231
BshFI GGCC 5 cut(s) 173, 230, 249, 285, 700
BshVI ATCGAT 1 cut(s) 261
BsiEI CGRYCG 1 cut(s) 231
BsiSI CCGG 3 cut(s) 227, 231, 336
BslFI GGGAC 2 cut(s) 158, 334
BslI CCNNNNNNNGG 4 cut(s) 203, 369, 398, 811
BsmAI GTCTC 3 cut(s) 203, 257, 716
BsmBI CGTCTC 1 cut(s) 257
BsmFI GGGAC 2 cut(s) 158, 334
BsmI GAATGC 2 cut(s) 610, 1068
BsnI GGCC 5 cut(s) 173, 230, 249, 285, 700
Bsp1407I TGTACA 1 cut(s) 51
Bsp143I GATC 4 cut(s) 458, 684, 838, 1132
BspACI CCGC 1 cut(s) 165
BspANI GGCC 5 cut(s) 173, 230, 249, 285, 700
BspCNI CTCAG 2 cut(s) 802, 1072
BspDI ATCGAT 1 cut(s) 261
BspLI GGNNCC 4 cut(s) 322, 670, 686, 903
BspMAI CTGCAG 1 cut(s) 358
BspPI GGATC 4 cut(s) 679, 692, 846, 1127
BsrFI RCCGGY 1 cut(s) 226
BsrGI TGTACA 1 cut(s) 51
BsrI ACTGG 3 cut(s) 166, 700, 1123
BssAI RCCGGY 1 cut(s) 226
BssECI CCNNGG 2 cut(s) 507, 562
BssMI GATC 4 cut(s) 458, 684, 838, 1132
BssT1I CCWWGG 2 cut(s) 507, 562
Bst4CI ACNGT 5 cut(s) 116, 130, 823, 997, 1003
BstAPI GCANNNNNTGC 2 cut(s) 347, 442
BstAUI TGTACA 1 cut(s) 51
BstBAI YACGTR 1 cut(s) 865
BstC8I GCNNGC 1 cut(s) 228
BstDEI CTNAG 4 cut(s) 296, 789, 830, 1080
BstF5I GGATG 4 cut(s) 176, 517, 989, 1069
BstHHI GCGC 2 cut(s) 302, 767
BstKTI GATC 4 cut(s) 461, 687, 841, 1135
BstMAI GTCTC 3 cut(s) 203, 257, 716
BstMBI GATC 4 cut(s) 458, 684, 838, 1132
BstMCI CGRYCG 1 cut(s) 231
BstMWI GCNNNNNNNGC 4 cut(s) 64, 347, 356, 442
BstNSI RCATGY 4 cut(s) 58, 436, 1157, 1161
BstPAI GACNNNNGTC 1 cut(s) 549
BstSFI CTRYAG 2 cut(s) 354, 967
BstV1I GCAGC 5 cut(s) 360, 368, 749, 838, 1097
BstX2I RGATCY 3 cut(s) 684, 838, 1132
BstXI CCANNNNNNTGG 1 cut(s) 695
BstYI RGATCY 3 cut(s) 684, 838, 1132
BstZI CGGCCG 1 cut(s) 228
Bsu15I ATCGAT 1 cut(s) 261
BsuRI GGCC 5 cut(s) 173, 230, 249, 285, 700
BsuTUI ATCGAT 1 cut(s) 261
BtsCI GGATG 4 cut(s) 176, 517, 989, 1069
BtsI GCAGTG 1 cut(s) 351
BtsIMutI CAGTG 3 cut(s) 351, 693, 1053
Cac8I GCNNGC 1 cut(s) 228
CaiI CAGNNNCTG 1 cut(s) 161
CfoI GCGC 2 cut(s) 302, 767
Cfr10I RCCGGY 1 cut(s) 226
Cfr13I GGNCC 2 cut(s) 321, 698
ClaI ATCGAT 1 cut(s) 261
Csp6I GTAC 3 cut(s) 52, 430, 517
CviAII CATG 5 cut(s) 55, 433, 442, 1154, 1158
CviQI GTAC 3 cut(s) 52, 430, 517
DdeI CTNAG 4 cut(s) 296, 789, 830, 1080
DpnI GATC 4 cut(s) 460, 686, 840, 1134
DpnII GATC 4 cut(s) 458, 684, 838, 1132
EaeI YGGCCR 3 cut(s) 228, 247, 283
EagI CGGCCG 1 cut(s) 228
EciI GGCGGA 1 cut(s) 180
EclXI CGGCCG 1 cut(s) 228
Eco130I CCWWGG 2 cut(s) 507, 562
Eco47I GGWCC 1 cut(s) 321
Eco52I CGGCCG 1 cut(s) 228
Eco57I CTGAAG 1 cut(s) 830
EcoT14I CCWWGG 2 cut(s) 507, 562
EcoT22I ATGCAT 2 cut(s) 610, 1159
ErhI CCWWGG 2 cut(s) 507, 562
Esp3I CGTCTC 1 cut(s) 257
FaeI CATG 5 cut(s) 58, 436, 445, 1157, 1161
FaqI GGGAC 2 cut(s) 158, 334
FatI CATG 5 cut(s) 54, 432, 441, 1153, 1157
Fnu4HI GCNGC 5 cut(s) 357, 374, 763, 852, 1086
FokI GGATG 4 cut(s) 163, 524, 996, 1076
Fsp4HI GCNGC 5 cut(s) 357, 374, 763, 852, 1086
FspI TGCGCA 1 cut(s) 766
GlaI GCGC 2 cut(s) 301, 766
GluI GCNGC 5 cut(s) 357, 374, 763, 852, 1086
GsuI CTGGAG 1 cut(s) 1140
HaeIII GGCC 5 cut(s) 173, 230, 249, 285, 700
HapII CCGG 3 cut(s) 227, 231, 336
HhaI GCGC 2 cut(s) 302, 767
Hin1II CATG 5 cut(s) 58, 436, 445, 1157, 1161
Hin6I GCGC 2 cut(s) 300, 765
HinP1I GCGC 2 cut(s) 300, 765
HindIII AAGCTT 1 cut(s) 528
HinfI GANTC 5 cut(s) 5, 146, 237, 311, 465
HpaII CCGG 3 cut(s) 227, 231, 336
HphI GGTGA 3 cut(s) 70, 218, 613
Hpy166II GTNNAC 2 cut(s) 500, 868
Hpy188I TCNGA 4 cut(s) 157, 555, 651, 1132
Hpy188III TCNNGA 3 cut(s) 456, 462, 617
Hpy8I GTNNAC 2 cut(s) 500, 868
HpyAV CCTTC 4 cut(s) 563, 805, 829, 1151
HpyCH4III ACNGT 5 cut(s) 116, 130, 823, 997, 1003
HpyCH4IV ACGT 1 cut(s) 864
HpyF10VI GCNNNNNNNGC 4 cut(s) 64, 347, 356, 442
HpyF3I CTNAG 4 cut(s) 296, 789, 830, 1080
HpySE526I ACGT 1 cut(s) 864
Hsp92II CATG 5 cut(s) 58, 436, 445, 1157, 1161
HspAI GCGC 2 cut(s) 300, 765
KroI GCCGGC 1 cut(s) 226
KroNI GCCGGC 1 cut(s) 228
Kzo9I GATC 4 cut(s) 458, 684, 838, 1132
LmnI GCTCC 2 cut(s) 668, 1121
Lsp1109I GCAGC 5 cut(s) 360, 368, 749, 838, 1097
LweI GCATC 2 cut(s) 82, 617
MaeII ACGT 1 cut(s) 864
MaeIII GTNAC 3 cut(s) 6, 997, 1160
MalI GATC 4 cut(s) 460, 686, 840, 1134
MboI GATC 4 cut(s) 458, 684, 838, 1132
MboII GAAGA 3 cut(s) 246, 406, 833
MfeI CAATTG 1 cut(s) 702
MflI RGATCY 3 cut(s) 684, 838, 1132
MlsI TGGCCA 1 cut(s) 285
MluCI AATT 8 cut(s) 15, 151, 343, 446, 702, 730, 1057, 1110
MluNI TGGCCA 1 cut(s) 285
MlyI GAGTC 3 cut(s) 14, 140, 459
MmeI TCCRAC 1 cut(s) 149
MnlI CCTC 9 cut(s) 103, 184, 207, 278, 473, 476, 592, 748, 1075
Mox20I TGGCCA 1 cut(s) 285
Mph1103I ATGCAT 2 cut(s) 610, 1159
MroNI GCCGGC 1 cut(s) 226
MscI TGGCCA 1 cut(s) 285
MseI TTAA 3 cut(s) 135, 798, 1148
Msp20I TGGCCA 1 cut(s) 285
MspI CCGG 3 cut(s) 227, 231, 336
MunI CAATTG 1 cut(s) 702
Mva1269I GAATGC 2 cut(s) 610, 1068
MwoI GCNNNNNNNGC 4 cut(s) 64, 347, 356, 442
NaeI GCCGGC 1 cut(s) 228
NdeII GATC 4 cut(s) 458, 684, 838, 1132
NgoMIV GCCGGC 1 cut(s) 226
NlaIII CATG 5 cut(s) 58, 436, 445, 1157, 1161
NlaIV GGNNCC 4 cut(s) 322, 670, 686, 903
NmuCI GTSAC 2 cut(s) 6, 1160
NsbI TGCGCA 1 cut(s) 766
NsiI ATGCAT 2 cut(s) 610, 1159
NspI RCATGY 4 cut(s) 58, 436, 1157, 1161
PctI GAATGC 2 cut(s) 610, 1068
PdiI GCCGGC 1 cut(s) 228
PfeI GAWTC 2 cut(s) 237, 311
PflMI CCANNNNNTGG 1 cut(s) 398
PkrI GCNGC 5 cut(s) 358, 375, 764, 853, 1087
PleI GAGTC 3 cut(s) 13, 140, 459
PpsI GAGTC 3 cut(s) 13, 140, 459
Ppu21I YACGTR 1 cut(s) 865
PshAI GACNNNNGTC 1 cut(s) 549
PspN4I GGNNCC 4 cut(s) 322, 670, 686, 903
PspPI GGNCC 2 cut(s) 321, 698
PsrI GAACNNNNNNTAC 4 cut(s) 422, 454, 982, 1014
PstI CTGCAG 1 cut(s) 358
PstNI CAGNNNCTG 1 cut(s) 161
PsuI RGATCY 3 cut(s) 684, 838, 1132
RsaI GTAC 3 cut(s) 53, 431, 518
RsaNI GTAC 3 cut(s) 52, 430, 517
SaqAI TTAA 3 cut(s) 135, 798, 1148
SatI GCNGC 5 cut(s) 357, 374, 763, 852, 1086
Sau3AI GATC 4 cut(s) 458, 684, 838, 1132
Sau96I GGNCC 2 cut(s) 321, 698
ScaI AGTACT 1 cut(s) 518
SchI GAGTC 3 cut(s) 14, 140, 459
SetI ASST 9 cut(s) 361, 532, 564, 603, 730, 745, 759, 867, 1090
SfaNI GCATC 2 cut(s) 82, 617
SfcI CTRYAG 2 cut(s) 354, 967
SinI GGWCC 1 cut(s) 321
Sse9I AATT 8 cut(s) 15, 151, 343, 446, 702, 730, 1057, 1110
SsiI CCGC 1 cut(s) 165
SspI AATATT 2 cut(s) 646, 1020
StyI CCWWGG 2 cut(s) 507, 562
TaaI ACNGT 5 cut(s) 116, 130, 823, 997, 1003
TaiI ACGT 1 cut(s) 867
TaqI TCGA 1 cut(s) 261
TasI AATT 8 cut(s) 15, 151, 343, 446, 702, 730, 1057, 1110
TatI WGTACW 3 cut(s) 51, 429, 516
TfiI GAWTC 2 cut(s) 237, 311
Tru1I TTAA 3 cut(s) 135, 798, 1148
Tru9I TTAA 3 cut(s) 135, 798, 1148
TscAI CASTG 3 cut(s) 358, 700, 1053
TseFI GTSAC 2 cut(s) 6, 1160
TseI GCWGC 5 cut(s) 356, 373, 762, 851, 1085
Tsp45I GTSAC 2 cut(s) 6, 1160
TspDTI ATGAA 4 cut(s) 27, 430, 795, 1061
TspRI CASTG 3 cut(s) 358, 700, 1053
Van91I CCANNNNNTGG 1 cut(s) 398
VpaK11BI GGWCC 1 cut(s) 321
XapI RAATTY 2 cut(s) 343, 1110
XceI RCATGY 4 cut(s) 58, 436, 1157, 1161
XcmI CCANNNNNNNNNTGG 1 cut(s) 1022
ZrmI AGTACT 1 cut(s) 518
Zsp2I ATGCAT 2 cut(s) 610, 1159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.