Belongs to the 'GDSL' lipolytic enzyme family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 2 cut(s) | 104, 385 |
| AcsI | RAATTY | 2 cut(s) | 352, 368 |
| AcuI | CTGAAG | 1 cut(s) | 88 |
| AfaI | GTAC | 1 cut(s) | 220 |
| AgsI | TTSAA | 5 cut(s) | 85, 156, 374, 408, 432 |
| AluBI | AGCT | 4 cut(s) | 46, 66, 183, 346 |
| AluI | AGCT | 4 cut(s) | 46, 66, 183, 346 |
| Alw21I | GWGCWC | 1 cut(s) | 185 |
| AlwI | GGATC | 2 cut(s) | 104, 385 |
| AoxI | GGCC | 1 cut(s) | 74 |
| ApeKI | GCWGC | 3 cut(s) | 109, 343, 346 |
| ApoI | RAATTY | 2 cut(s) | 352, 368 |
| BanII | GRGCYC | 1 cut(s) | 185 |
| BauI | CACGAG | 1 cut(s) | 61 |
| Bbv12I | GWGCWC | 1 cut(s) | 185 |
| BbvI | GCAGC | 3 cut(s) | 96, 333, 355 |
| BccI | CCATC | 1 cut(s) | 316 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 328, 362 |
| BisI | GCNGC | 3 cut(s) | 110, 344, 347 |
| BlsI | GCNGC | 3 cut(s) | 111, 345, 348 |
| BmiI | GGNNCC | 2 cut(s) | 161, 207 |
| BmrI | ACTGGG | 1 cut(s) | 327 |
| BmuI | ACTGGG | 1 cut(s) | 327 |
| BpmI | CTGGAG | 1 cut(s) | 398 |
| BsaJI | CCNNGG | 1 cut(s) | 338 |
| BsaWI | WCCGGW | 1 cut(s) | 208 |
| Bse1I | ACTGG | 2 cut(s) | 322, 381 |
| BseDI | CCNNGG | 1 cut(s) | 338 |
| BseGI | GGATG | 1 cut(s) | 327 |
| BseMII | CTCAG | 1 cut(s) | 102 |
| BseNI | ACTGG | 2 cut(s) | 322, 381 |
| BseXI | GCAGC | 3 cut(s) | 96, 333, 355 |
| BshFI | GGCC | 1 cut(s) | 76 |
| BsiHKAI | GWGCWC | 1 cut(s) | 185 |
| BsiSI | CCGG | 1 cut(s) | 209 |
| BsnI | GGCC | 1 cut(s) | 76 |
| Bsp1286I | GDGCHC | 1 cut(s) | 185 |
| Bsp143I | GATC | 2 cut(s) | 96, 390 |
| BspANI | GGCC | 1 cut(s) | 76 |
| BspCNI | CTCAG | 1 cut(s) | 101 |
| BspLI | GGNNCC | 2 cut(s) | 161, 207 |
| BspPI | GGATC | 2 cut(s) | 104, 385 |
| BsrI | ACTGG | 2 cut(s) | 322, 381 |
| BssECI | CCNNGG | 1 cut(s) | 338 |
| BssMI | GATC | 2 cut(s) | 96, 390 |
| BssSI | CACGAG | 1 cut(s) | 61 |
| Bst2BI | CACGAG | 1 cut(s) | 61 |
| Bst4CI | ACNGT | 1 cut(s) | 261 |
| Bst6I | CTCTTC | 1 cut(s) | 404 |
| BstC8I | GCNNGC | 1 cut(s) | 74 |
| BstDEI | CTNAG | 2 cut(s) | 2, 88 |
| BstF5I | GGATG | 1 cut(s) | 327 |
| BstKTI | GATC | 2 cut(s) | 99, 393 |
| BstMBI | GATC | 2 cut(s) | 96, 390 |
| BstNSI | RCATGY | 1 cut(s) | 419 |
| BstV1I | GCAGC | 3 cut(s) | 96, 333, 355 |
| BstX2I | RGATCY | 2 cut(s) | 96, 390 |
| BstYI | RGATCY | 2 cut(s) | 96, 390 |
| BsuRI | GGCC | 1 cut(s) | 76 |
| BtsCI | GGATG | 1 cut(s) | 327 |
| BtsI | GCAGTG | 1 cut(s) | 233 |
| BtsIMutI | CAGTG | 1 cut(s) | 233 |
| Cac8I | GCNNGC | 1 cut(s) | 74 |
| Csp6I | GTAC | 1 cut(s) | 219 |
| CviAII | CATG | 2 cut(s) | 290, 416 |
| CviQI | GTAC | 1 cut(s) | 219 |
| DdeI | CTNAG | 2 cut(s) | 2, 88 |
| DpnI | GATC | 2 cut(s) | 98, 392 |
| DpnII | GATC | 2 cut(s) | 96, 390 |
| Eam1104I | CTCTTC | 1 cut(s) | 404 |
| EarI | CTCTTC | 1 cut(s) | 404 |
| Ecl136II | GAGCTC | 1 cut(s) | 183 |
| Eco24I | GRGCYC | 1 cut(s) | 185 |
| Eco53kI | GAGCTC | 1 cut(s) | 183 |
| Eco57I | CTGAAG | 1 cut(s) | 88 |
| EcoICRI | GAGCTC | 1 cut(s) | 183 |
| EcoT38I | GRGCYC | 1 cut(s) | 185 |
| FaeI | CATG | 2 cut(s) | 293, 419 |
| FaiI | YATR | 6 cut(s) | 79, 122, 173, 227, 291, 417 |
| FatI | CATG | 2 cut(s) | 289, 415 |
| Fnu4HI | GCNGC | 3 cut(s) | 110, 344, 347 |
| FokI | GGATG | 1 cut(s) | 334 |
| FriOI | GRGCYC | 1 cut(s) | 185 |
| Fsp4HI | GCNGC | 3 cut(s) | 110, 344, 347 |
| GluI | GCNGC | 3 cut(s) | 110, 344, 347 |
| GsuI | CTGGAG | 1 cut(s) | 398 |
| HaeIII | GGCC | 1 cut(s) | 76 |
| HapII | CCGG | 1 cut(s) | 209 |
| Hin1II | CATG | 2 cut(s) | 293, 419 |
| HpaII | CCGG | 1 cut(s) | 209 |
| Hpy166II | GTNNAC | 1 cut(s) | 315 |
| Hpy188I | TCNGA | 1 cut(s) | 390 |
| Hpy188III | TCNNGA | 1 cut(s) | 90 |
| Hpy8I | GTNNAC | 1 cut(s) | 315 |
| HpyAV | CCTTC | 1 cut(s) | 87 |
| HpyCH4III | ACNGT | 1 cut(s) | 261 |
| HpyCH4V | TGCA | 3 cut(s) | 25, 112, 293 |
| HpyF3I | CTNAG | 2 cut(s) | 2, 88 |
| Hsp92II | CATG | 2 cut(s) | 293, 419 |
| Kzo9I | GATC | 2 cut(s) | 96, 390 |
| LmnI | GCTCC | 4 cut(s) | 188, 211, 247, 379 |
| LpnPI | CCDG | 6 cut(s) | 58, 75, 222, 303, 362, 408 |
| Lsp1109I | GCAGC | 3 cut(s) | 96, 333, 355 |
| MaeIII | GTNAC | 1 cut(s) | 418 |
| MalI | GATC | 2 cut(s) | 98, 392 |
| MboI | GATC | 2 cut(s) | 96, 390 |
| MboII | GAAGA | 2 cut(s) | 91, 391 |
| MflI | RGATCY | 2 cut(s) | 96, 390 |
| MhlI | GDGCHC | 1 cut(s) | 185 |
| MluCI | AATT | 4 cut(s) | 254, 305, 352, 368 |
| MnlI | CCTC | 3 cut(s) | 312, 333, 407 |
| MseI | TTAA | 1 cut(s) | 56 |
| MspA1I | CMGCKG | 1 cut(s) | 346 |
| MspI | CCGG | 1 cut(s) | 209 |
| NdeII | GATC | 2 cut(s) | 96, 390 |
| NlaIII | CATG | 2 cut(s) | 293, 419 |
| NlaIV | GGNNCC | 2 cut(s) | 161, 207 |
| NmuCI | GTSAC | 1 cut(s) | 418 |
| NspI | RCATGY | 1 cut(s) | 419 |
| PkrI | GCNGC | 3 cut(s) | 111, 345, 348 |
| Psp124BI | GAGCTC | 1 cut(s) | 185 |
| PspN4I | GGNNCC | 2 cut(s) | 161, 207 |
| PsuI | RGATCY | 2 cut(s) | 96, 390 |
| PvuII | CAGCTG | 1 cut(s) | 346 |
| RsaI | GTAC | 1 cut(s) | 220 |
| RsaNI | GTAC | 1 cut(s) | 219 |
| SacI | GAGCTC | 1 cut(s) | 185 |
| SaqAI | TTAA | 1 cut(s) | 56 |
| SatI | GCNGC | 3 cut(s) | 110, 344, 347 |
| Sau3AI | GATC | 2 cut(s) | 96, 390 |
| SduI | GDGCHC | 1 cut(s) | 185 |
| SetI | ASST | 5 cut(s) | 48, 68, 185, 348, 399 |
| Sse9I | AATT | 4 cut(s) | 254, 305, 352, 368 |
| SstI | GAGCTC | 1 cut(s) | 185 |
| TaaI | ACNGT | 1 cut(s) | 261 |
| TasI | AATT | 4 cut(s) | 254, 305, 352, 368 |
| TatI | WGTACW | 1 cut(s) | 218 |
| Tru1I | TTAA | 1 cut(s) | 56 |
| Tru9I | TTAA | 1 cut(s) | 56 |
| TscAI | CASTG | 1 cut(s) | 240 |
| TseFI | GTSAC | 1 cut(s) | 418 |
| TseI | GCWGC | 3 cut(s) | 109, 343, 346 |
| Tsp45I | GTSAC | 1 cut(s) | 418 |
| TspDTI | ATGAA | 3 cut(s) | 53, 278, 319 |
| TspRI | CASTG | 1 cut(s) | 240 |
| XapI | RAATTY | 2 cut(s) | 352, 368 |
| XceI | RCATGY | 1 cut(s) | 419 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 280 |