Rh6BG375300

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000978
Physical Location & Seq
Reverse (-)
60468994 .. 60471052
2059 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG375300.1

Sequence Viewer

Length: 312 bp
ATGCCGGTCGGAAGATTCTCTGATGGAAGACTCATTATCGACTTCCTTGGATTTGAGCTCCCACTTGTTGCTTGTTGTGGCTCCGGTGGTAAGTACAACTATAACGCCACTGCTGGATGTGGAGCAATAATTACAGTTAATGGAAGCCAAATCCTTGTTGGTTCATGCAAAAACCCCTCAATTAGAGTGAACTGGGATGGTATTCATTACACCGAGGCAGCTGCGAAATTTGTTTTTGATAAAATTTCAACCGGAGCGTATTCAGATCCACCTCTTCCCTTGAAACAAGCATGTCACAGGAGTTTGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005488 GO:0006950 GO:0006952 GO:0008150 GO:0016787 GO:0016788 GO:0019863 GO:0019865 GO:0044877 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

103

Amino Acids

10.97

Weight (kDa)

8.32

Isoelectric Point (pI)

45.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 260
AcsI RAATTY 2 cut(s) 227, 243
AfaI GTAC 1 cut(s) 95
AgsI TTSAA 3 cut(s) 249, 283, 307
AluBI AGCT 2 cut(s) 58, 221
AluI AGCT 2 cut(s) 58, 221
Alw21I GWGCWC 1 cut(s) 60
AlwI GGATC 1 cut(s) 260
ApeKI GCWGC 2 cut(s) 218, 221
ApoI RAATTY 2 cut(s) 227, 243
BanII GRGCYC 1 cut(s) 60
BbsI GAAGAC 1 cut(s) 34
Bbv12I GWGCWC 1 cut(s) 60
BbvI GCAGC 2 cut(s) 208, 230
BccI CCATC 2 cut(s) 17, 191
BcgI CGANNNNNNTGC 2 cut(s) 203, 237
BisI GCNGC 2 cut(s) 219, 222
BlsI GCNGC 2 cut(s) 220, 223
BmiI GGNNCC 1 cut(s) 82
BmrI ACTGGG 1 cut(s) 202
BmuI ACTGGG 1 cut(s) 202
BpiI GAAGAC 1 cut(s) 34
BsaJI CCNNGG 2 cut(s) 46, 213
BsaWI WCCGGW 2 cut(s) 83, 251
Bse118I RCCGGY 1 cut(s) 4
Bse1I ACTGG 1 cut(s) 197
BseDI CCNNGG 2 cut(s) 46, 213
BseGI GGATG 2 cut(s) 122, 202
BseNI ACTGG 1 cut(s) 197
BseXI GCAGC 2 cut(s) 208, 230
Bsh1285I CGRYCG 1 cut(s) 9
BsiEI CGRYCG 1 cut(s) 9
BsiHKAI GWGCWC 1 cut(s) 60
BsiSI CCGG 3 cut(s) 5, 84, 252
Bsp1286I GDGCHC 1 cut(s) 60
Bsp143I GATC 1 cut(s) 265
BspLI GGNNCC 1 cut(s) 82
BspPI GGATC 1 cut(s) 260
BsrFI RCCGGY 1 cut(s) 4
BsrI ACTGG 1 cut(s) 197
BssAI RCCGGY 1 cut(s) 4
BssECI CCNNGG 2 cut(s) 46, 213
BssMI GATC 1 cut(s) 265
BssT1I CCWWGG 1 cut(s) 46
Bst4CI ACNGT 1 cut(s) 136
Bst6I CTCTTC 1 cut(s) 279
BstF5I GGATG 2 cut(s) 122, 202
BstKTI GATC 1 cut(s) 268
BstMBI GATC 1 cut(s) 265
BstMCI CGRYCG 1 cut(s) 9
BstNSI RCATGY 1 cut(s) 294
BstV1I GCAGC 2 cut(s) 208, 230
BstV2I GAAGAC 1 cut(s) 34
BstX2I RGATCY 1 cut(s) 265
BstYI RGATCY 1 cut(s) 265
BtsCI GGATG 2 cut(s) 122, 202
BtsI GCAGTG 1 cut(s) 108
BtsIMutI CAGTG 1 cut(s) 108
Cfr10I RCCGGY 1 cut(s) 4
Csp6I GTAC 1 cut(s) 94
CviAII CATG 2 cut(s) 165, 291
CviJI RGCY 4 cut(s) 58, 81, 147, 221
CviKI_1 RGCY 4 cut(s) 58, 81, 147, 221
CviQI GTAC 1 cut(s) 94
DpnI GATC 1 cut(s) 267
DpnII GATC 1 cut(s) 265
Eam1104I CTCTTC 1 cut(s) 279
EarI CTCTTC 1 cut(s) 279
Ecl136II GAGCTC 1 cut(s) 58
Eco130I CCWWGG 1 cut(s) 46
Eco24I GRGCYC 1 cut(s) 60
Eco53kI GAGCTC 1 cut(s) 58
EcoICRI GAGCTC 1 cut(s) 58
EcoT14I CCWWGG 1 cut(s) 46
EcoT38I GRGCYC 1 cut(s) 60
ErhI CCWWGG 1 cut(s) 46
FaeI CATG 2 cut(s) 168, 294
FaiI YATR 3 cut(s) 102, 166, 292
FatI CATG 2 cut(s) 164, 290
Fnu4HI GCNGC 2 cut(s) 219, 222
FokI GGATG 2 cut(s) 129, 209
FriOI GRGCYC 1 cut(s) 60
Fsp4HI GCNGC 2 cut(s) 219, 222
GluI GCNGC 2 cut(s) 219, 222
HapII CCGG 3 cut(s) 5, 84, 252
Hin1II CATG 2 cut(s) 168, 294
HinfI GANTC 2 cut(s) 15, 30
HpaII CCGG 3 cut(s) 5, 84, 252
Hpy166II GTNNAC 1 cut(s) 190
Hpy188I TCNGA 3 cut(s) 11, 22, 265
Hpy8I GTNNAC 1 cut(s) 190
HpyCH4III ACNGT 1 cut(s) 136
HpyCH4V TGCA 1 cut(s) 168
Hsp92II CATG 2 cut(s) 168, 294
Kzo9I GATC 1 cut(s) 265
LmnI GCTCC 4 cut(s) 63, 86, 122, 254
LpnPI CCDG 6 cut(s) 18, 97, 99, 178, 265, 283
Lsp1109I GCAGC 2 cut(s) 208, 230
MaeIII GTNAC 1 cut(s) 293
MalI GATC 1 cut(s) 267
MboI GATC 1 cut(s) 265
MboII GAAGA 3 cut(s) 24, 39, 266
MflI RGATCY 1 cut(s) 265
MhlI GDGCHC 1 cut(s) 60
MluCI AATT 4 cut(s) 129, 180, 227, 243
MlyI GAGTC 1 cut(s) 24
MnlI CCTC 3 cut(s) 187, 208, 282
MseI TTAA 1 cut(s) 138
MspA1I CMGCKG 1 cut(s) 221
MspI CCGG 3 cut(s) 5, 84, 252
NdeII GATC 1 cut(s) 265
NlaIII CATG 2 cut(s) 168, 294
NlaIV GGNNCC 1 cut(s) 82
NmuCI GTSAC 1 cut(s) 293
NspI RCATGY 1 cut(s) 294
PfeI GAWTC 1 cut(s) 15
PkrI GCNGC 2 cut(s) 220, 223
PleI GAGTC 1 cut(s) 24
PpsI GAGTC 1 cut(s) 24
Psp124BI GAGCTC 1 cut(s) 60
PspN4I GGNNCC 1 cut(s) 82
PsuI RGATCY 1 cut(s) 265
PvuII CAGCTG 1 cut(s) 221
RsaI GTAC 1 cut(s) 95
RsaNI GTAC 1 cut(s) 94
SacI GAGCTC 1 cut(s) 60
SaqAI TTAA 1 cut(s) 138
SatI GCNGC 2 cut(s) 219, 222
Sau3AI GATC 1 cut(s) 265
SchI GAGTC 1 cut(s) 24
SduI GDGCHC 1 cut(s) 60
SetI ASST 3 cut(s) 60, 223, 274
Sse9I AATT 4 cut(s) 129, 180, 227, 243
SstI GAGCTC 1 cut(s) 60
StyI CCWWGG 1 cut(s) 46
TaaI ACNGT 1 cut(s) 136
TaqI TCGA 1 cut(s) 39
TasI AATT 4 cut(s) 129, 180, 227, 243
TatI WGTACW 1 cut(s) 93
TfiI GAWTC 1 cut(s) 15
Tru1I TTAA 1 cut(s) 138
Tru9I TTAA 1 cut(s) 138
TscAI CASTG 1 cut(s) 115
TseFI GTSAC 1 cut(s) 293
TseI GCWGC 2 cut(s) 218, 221
Tsp45I GTSAC 1 cut(s) 293
TspDTI ATGAA 2 cut(s) 153, 194
TspRI CASTG 1 cut(s) 115
XapI RAATTY 2 cut(s) 227, 243
XceI RCATGY 1 cut(s) 294
XcmI CCANNNNNNNNNTGG 1 cut(s) 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.