Rw6G032070

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000978
Physical Location & Seq
Reverse (-)
56181699 .. 56184949
3251 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G032070.1

Sequence Viewer

Length: 1278 bp
ATGCCGCGTCGAAATACAAATGAAGTAAATTGCACGATACGTACATTACACTATATAAAACAAACACACATTAGTTTCTCGATCATCTCCATCTTTTGGTATTTCTCCACCATGGGGTACTCTCCTAAAGTTATCGTCTCTCTGTTCTGCTCCTATATGCTTATATTGCTTATTACATGTAGTGCTGTGATCCCTGCCTCGGCTTTGAAAGACTGTGAGTTTCCTGCCATCTTCAACTTCGGAGACTCAAATTCAGATACTGGTGGACTAGCCGCATCCCTTCTACCACCAACCACACCTTATGGAGAGACCTATTTCCATATGCCGGCCGGAAGATTCTCTGATGGAAGACTCATCATCGATTTCCTTGCAAAGAGTCTTGGTCACCCTTATCTAAGCGCATATCTGGATTCTCTCGGGACAAATTTCTCGTATGGAGCAAATTTTGCAACTGCAGCTTCCACTATCAGACTCCCAGATCTCATTATACCAGCCGGTGGATTTAGTCCCTTCTACCTTGATATTCAATATATGCAGTTCCTGCAACTAAAATCCAGATCACAACTCATAAGGCATCGAGGGGGAATATTTGCAAGTTTAATGCCCAAGGAGGAGTACTTCTCCAAAGCTTTATACACATTCGACATTGGCCAGAATGATCTTGGCGAGGGATTTTTCGGTGACCTGACTATACAGGAAGTAAATGCATCTGTTCCTGATATAATCACTGGTTTCTCAACTAATATCAAGAAAATATATGATTTGGGAGCAAGATCATTATGGATCCACAATACGGGGCCGATTGGCTGTCTCCCTTACATTGTGGCAAACTTCCCGTCAGCTGAGAAAGATGAGATTGGCTGTGCAAAGGCTTACAATGAAGTAGCTCAACATTTTAACCACGAGCTGAAGCAGGCCATAGTTCAACTCAGGAAGGATCTTCCTTTGGCTGCATTCACTTATGTAGATGTGTATTCTGTCAAATACTCTCTTTTCAAGGAACCCGAAAAGTATGGATTTGAGCTCCCTCTTGTTGCTTGCTGTGGCTCCGGTGGTAAGTACAACTATAACGCCACTGCTGGATGTGGAGCAATAATTACAGTCAATGGAAGCCAAATCCTTGTTGGTTCATGCAAAAACCCCTCAATTAGAGTGAACTGGGATGGTATTCATTACACCGAGGCAGCTGCGAAATTTGTTTTTGATAAAATTTCAACTGGAGCGTATTCAGATCCACCTCTTCCCTTGAAACAAGCATGTCACAGGAGTTTGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005488 GO:0006950 GO:0006952 GO:0008150 GO:0016787 GO:0016788 GO:0019863 GO:0019865 GO:0044877 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

425

Amino Acids

46.91

Weight (kDa)

7.1

Isoelectric Point (pI)

40.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 77 - 404 4.2e-46 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 96, 497
AccII CGCG 1 cut(s) 7
AciI CCGC 2 cut(s) 5, 273
AclWI GGATC 5 cut(s) 184, 778, 791, 945, 1226
AcoI YGGCCR 2 cut(s) 327, 649
AcsI RAATTY 5 cut(s) 250, 424, 442, 1193, 1209
AcuI CTGAAG 1 cut(s) 929
AfaI GTAC 4 cut(s) 43, 119, 617, 1061
AfiI CCNNNNNNNGG 6 cut(s) 96, 114, 199, 325, 497, 793
AflIII ACRYGT 1 cut(s) 176
AgsI TTSAA 8 cut(s) 208, 235, 527, 926, 997, 1215, 1249, 1273
AluBI AGCT 7 cut(s) 458, 629, 842, 887, 907, 1024, 1187
AluI AGCT 7 cut(s) 458, 629, 842, 887, 907, 1024, 1187
Alw21I GWGCWC 1 cut(s) 1026
Alw26I GTCTC 4 cut(s) 142, 237, 302, 815
AlwI GGATC 5 cut(s) 184, 778, 791, 945, 1226
AlwNI CAGNNNCTG 2 cut(s) 260, 541
Ama87I CYCGRG 1 cut(s) 416
AoxI GGCC 4 cut(s) 327, 649, 797, 915
ApeKI GCWGC 4 cut(s) 455, 950, 1184, 1187
ApoI RAATTY 5 cut(s) 250, 424, 442, 1193, 1209
ArsI GACNNNNNNTTYG 2 cut(s) 821, 853
AspLEI GCGC 1 cut(s) 401
AspS9I GGNCC 1 cut(s) 797
AsuHPI GGTGA 2 cut(s) 377, 692
AvaI CYCGRG 1 cut(s) 416
BalI TGGCCA 1 cut(s) 651
BamHI GGATCC 1 cut(s) 783
BanII GRGCYC 1 cut(s) 1026
BauI CACGAG 1 cut(s) 902
BbsI GAAGAC 1 cut(s) 355
Bbv12I GWGCWC 1 cut(s) 1026
BbvI GCAGC 4 cut(s) 467, 937, 1174, 1196
BccI CCATC 4 cut(s) 98, 236, 338, 1157
BcgI CGANNNNNNTGC 4 cut(s) 350, 384, 1169, 1203
BcoDI GTCTC 4 cut(s) 142, 237, 302, 815
BfaI CTAG 1 cut(s) 269
BfmI CTRYAG 1 cut(s) 453
BglII AGATCT 1 cut(s) 478
BisI GCNGC 6 cut(s) 5, 273, 456, 951, 1185, 1188
BlsI GCNGC 6 cut(s) 6, 274, 457, 952, 1186, 1189
BmcAI AGTACT 1 cut(s) 617
BmeT110I CYCGRG 1 cut(s) 416
BmgT120I GGNCC 1 cut(s) 797
BmiI GGNNCC 4 cut(s) 785, 798, 1002, 1048
BmrI ACTGGG 1 cut(s) 1168
BmsI GCATC 3 cut(s) 284, 583, 716
BmuI ACTGGG 1 cut(s) 1168
BpiI GAAGAC 1 cut(s) 355
BplI GAGNNNNNCTC 2 cut(s) 605, 637
BpmI CTGGAG 1 cut(s) 1239
Bsa29I ATCGAT 1 cut(s) 360
BsaAI YACGTR 1 cut(s) 41
BsaI GGTCTC 1 cut(s) 302
BsaJI CCNNGG 4 cut(s) 111, 198, 606, 1179
BsaWI WCCGGW 1 cut(s) 1049
Bsc4I CCNNNNNNNGG 6 cut(s) 96, 114, 199, 325, 497, 793
Bse118I RCCGGY 2 cut(s) 325, 494
Bse1I ACTGG 4 cut(s) 265, 733, 1163, 1222
BseCI ATCGAT 1 cut(s) 360
BseDI CCNNGG 4 cut(s) 111, 198, 606, 1179
BseGI GGATG 3 cut(s) 275, 1088, 1168
BseLI CCNNNNNNNGG 6 cut(s) 96, 114, 199, 325, 497, 793
BseMII CTCAG 2 cut(s) 834, 943
BseNI ACTGG 4 cut(s) 265, 733, 1163, 1222
BseRI GAGGAG 1 cut(s) 626
BseX3I CGGCCG 1 cut(s) 327
BseXI GCAGC 4 cut(s) 467, 937, 1174, 1196
Bsh1236I CGCG 1 cut(s) 7
Bsh1285I CGRYCG 1 cut(s) 330
BshFI GGCC 4 cut(s) 329, 651, 799, 917
BshVI ATCGAT 1 cut(s) 360
BsiEI CGRYCG 1 cut(s) 330
BsiHKAI GWGCWC 1 cut(s) 1026
BsiHKCI CYCGRG 1 cut(s) 416
BsiSI CCGG 4 cut(s) 326, 330, 495, 1050
BslFI GGGAC 2 cut(s) 433, 492
BslI CCNNNNNNNGG 6 cut(s) 96, 114, 199, 325, 497, 793
BsmAI GTCTC 4 cut(s) 142, 237, 302, 815
BsmBI CGTCTC 1 cut(s) 142
BsmFI GGGAC 2 cut(s) 433, 492
BsmI GAATGC 1 cut(s) 953
BsnI GGCC 4 cut(s) 329, 651, 799, 917
Bso31I GGTCTC 1 cut(s) 302
BsoBI CYCGRG 1 cut(s) 416
Bsp1286I GDGCHC 1 cut(s) 1026
Bsp143I GATC 9 cut(s) 81, 189, 478, 557, 658, 773, 783, 937, 1231
Bsp19I CCATGG 1 cut(s) 111
BspACI CCGC 2 cut(s) 5, 273
BspANI GGCC 4 cut(s) 329, 651, 799, 917
BspCNI CTCAG 2 cut(s) 835, 942
BspDI ATCGAT 1 cut(s) 360
BspFNI CGCG 1 cut(s) 7
BspLI GGNNCC 4 cut(s) 785, 798, 1002, 1048
BspMAI CTGCAG 1 cut(s) 457
BspPI GGATC 5 cut(s) 184, 778, 791, 945, 1226
BspTNI GGTCTC 1 cut(s) 302
BsrFI RCCGGY 2 cut(s) 325, 494
BsrI ACTGG 4 cut(s) 265, 733, 1163, 1222
BssAI RCCGGY 2 cut(s) 325, 494
BssECI CCNNGG 4 cut(s) 111, 198, 606, 1179
BssMI GATC 9 cut(s) 81, 189, 478, 557, 658, 773, 783, 937, 1231
BssSI CACGAG 1 cut(s) 902
BssT1I CCWWGG 2 cut(s) 111, 606
Bst2BI CACGAG 1 cut(s) 902
Bst4CI ACNGT 2 cut(s) 215, 1102
Bst6I CTCTTC 1 cut(s) 1245
BstAPI GCANNNNNTGC 2 cut(s) 446, 541
BstBAI YACGTR 1 cut(s) 41
BstC8I GCNNGC 3 cut(s) 327, 915, 1039
BstDEI CTNAG 3 cut(s) 395, 843, 929
BstDSI CCRYGG 1 cut(s) 111
BstEII GGTNACC 2 cut(s) 383, 680
BstF5I GGATG 3 cut(s) 275, 1088, 1168
BstFNI CGCG 1 cut(s) 7
BstHHI GCGC 1 cut(s) 401
BstKTI GATC 9 cut(s) 84, 192, 481, 560, 661, 776, 786, 940, 1234
BstMAI GTCTC 4 cut(s) 142, 237, 302, 815
BstMBI GATC 9 cut(s) 81, 189, 478, 557, 658, 773, 783, 937, 1231
BstMCI CGRYCG 1 cut(s) 330
BstMWI GCNNNNNNNGC 4 cut(s) 166, 446, 455, 541
BstNSI RCATGY 2 cut(s) 180, 1260
BstPI GGTNACC 2 cut(s) 383, 680
BstSFI CTRYAG 1 cut(s) 453
BstSNI TACGTA 1 cut(s) 41
BstUI CGCG 1 cut(s) 7
BstV1I GCAGC 4 cut(s) 467, 937, 1174, 1196
BstV2I GAAGAC 1 cut(s) 355
BstX2I RGATCY 4 cut(s) 478, 783, 937, 1231
BstYI RGATCY 4 cut(s) 478, 783, 937, 1231
BstZI CGGCCG 1 cut(s) 327
Bsu15I ATCGAT 1 cut(s) 360
BsuRI GGCC 4 cut(s) 329, 651, 799, 917
BsuTUI ATCGAT 1 cut(s) 360
BtgI CCRYGG 1 cut(s) 111
BtsCI GGATG 3 cut(s) 275, 1088, 1168
BtsI GCAGTG 1 cut(s) 1074
BtsIMutI CAGTG 2 cut(s) 726, 1074
Cac8I GCNNGC 3 cut(s) 327, 915, 1039
CaiI CAGNNNCTG 2 cut(s) 260, 541
CfoI GCGC 1 cut(s) 401
Cfr10I RCCGGY 2 cut(s) 325, 494
Cfr13I GGNCC 1 cut(s) 797
ClaI ATCGAT 1 cut(s) 360
Csp6I GTAC 4 cut(s) 42, 118, 616, 1060
CviAII CATG 4 cut(s) 112, 177, 1131, 1257
CviQI GTAC 4 cut(s) 42, 118, 616, 1060
DdeI CTNAG 3 cut(s) 395, 843, 929
DpnI GATC 9 cut(s) 83, 191, 480, 559, 660, 775, 785, 939, 1233
DpnII GATC 9 cut(s) 81, 189, 478, 557, 658, 773, 783, 937, 1231
EaeI YGGCCR 2 cut(s) 327, 649
EagI CGGCCG 1 cut(s) 327
Eam1104I CTCTTC 1 cut(s) 1245
EarI CTCTTC 1 cut(s) 1245
Ecl136II GAGCTC 1 cut(s) 1024
EclXI CGGCCG 1 cut(s) 327
Eco105I TACGTA 1 cut(s) 41
Eco130I CCWWGG 2 cut(s) 111, 606
Eco24I GRGCYC 1 cut(s) 1026
Eco31I GGTCTC 1 cut(s) 302
Eco52I CGGCCG 1 cut(s) 327
Eco53kI GAGCTC 1 cut(s) 1024
Eco57I CTGAAG 1 cut(s) 929
Eco88I CYCGRG 1 cut(s) 416
Eco91I GGTNACC 2 cut(s) 383, 680
EcoICRI GAGCTC 1 cut(s) 1024
EcoO65I GGTNACC 2 cut(s) 383, 680
EcoT14I CCWWGG 2 cut(s) 111, 606
EcoT22I ATGCAT 1 cut(s) 709
EcoT38I GRGCYC 1 cut(s) 1026
ErhI CCWWGG 2 cut(s) 111, 606
Esp3I CGTCTC 1 cut(s) 142
FaeI CATG 4 cut(s) 115, 180, 1134, 1260
FaqI GGGAC 2 cut(s) 433, 492
FatI CATG 4 cut(s) 111, 176, 1130, 1256
FauNDI CATATG 1 cut(s) 321
Fnu4HI GCNGC 6 cut(s) 5, 273, 456, 951, 1185, 1188
FokI GGATG 3 cut(s) 262, 1095, 1175
FriOI GRGCYC 1 cut(s) 1026
Fsp4HI GCNGC 6 cut(s) 5, 273, 456, 951, 1185, 1188
FspBI CTAG 1 cut(s) 269
GlaI GCGC 1 cut(s) 400
GluI GCNGC 6 cut(s) 5, 273, 456, 951, 1185, 1188
GsuI CTGGAG 1 cut(s) 1239
HaeIII GGCC 4 cut(s) 329, 651, 799, 917
HapII CCGG 4 cut(s) 326, 330, 495, 1050
HhaI GCGC 1 cut(s) 401
Hin1II CATG 4 cut(s) 115, 180, 1134, 1260
Hin6I GCGC 1 cut(s) 399
HinP1I GCGC 1 cut(s) 399
HindIII AAGCTT 1 cut(s) 627
HinfI GANTC 6 cut(s) 245, 336, 351, 376, 410, 471
HpaII CCGG 4 cut(s) 326, 330, 495, 1050
HphI GGTGA 2 cut(s) 377, 692
Hpy166II GTNNAC 2 cut(s) 266, 1156
Hpy188I TCNGA 5 cut(s) 242, 256, 343, 470, 1231
Hpy188III TCNNGA 7 cut(s) 79, 407, 418, 555, 716, 748, 931
Hpy8I GTNNAC 2 cut(s) 266, 1156
Hpy99I CGWCG 1 cut(s) 12
HpyAV CCTTC 3 cut(s) 290, 520, 928
HpyCH4III ACNGT 2 cut(s) 215, 1102
HpyCH4IV ACGT 1 cut(s) 40
HpyF10VI GCNNNNNNNGC 4 cut(s) 166, 446, 455, 541
HpyF3I CTNAG 3 cut(s) 395, 843, 929
HpySE526I ACGT 1 cut(s) 40
Hsp92II CATG 4 cut(s) 115, 180, 1134, 1260
HspAI GCGC 1 cut(s) 399
KroI GCCGGC 1 cut(s) 325
KroNI GCCGGC 1 cut(s) 327
Kzo9I GATC 9 cut(s) 81, 189, 478, 557, 658, 773, 783, 937, 1231
LmnI GCTCC 7 cut(s) 155, 437, 767, 1029, 1052, 1088, 1220
Lsp1109I GCAGC 4 cut(s) 467, 937, 1174, 1196
LweI GCATC 3 cut(s) 284, 583, 716
MaeI CTAG 1 cut(s) 269
MaeII ACGT 1 cut(s) 40
MaeIII GTNAC 3 cut(s) 383, 680, 1259
MalI GATC 9 cut(s) 83, 191, 480, 559, 660, 775, 785, 939, 1233
MboI GATC 9 cut(s) 81, 189, 478, 557, 658, 773, 783, 937, 1231
MboII GAAGA 5 cut(s) 223, 345, 360, 932, 1232
MflI RGATCY 4 cut(s) 478, 783, 937, 1231
MhlI GDGCHC 1 cut(s) 1026
MlsI TGGCCA 1 cut(s) 651
MluCI AATT 8 cut(s) 28, 250, 424, 442, 1095, 1146, 1193, 1209
MluNI TGGCCA 1 cut(s) 651
MlyI GAGTC 4 cut(s) 239, 345, 385, 465
MnlI CCTC 8 cut(s) 208, 572, 604, 661, 1038, 1153, 1174, 1248
Mox20I TGGCCA 1 cut(s) 651
Mph1103I ATGCAT 1 cut(s) 709
MroNI GCCGGC 1 cut(s) 325
MscI TGGCCA 1 cut(s) 651
MseI TTAA 2 cut(s) 599, 897
Msp20I TGGCCA 1 cut(s) 651
MspA1I CMGCKG 2 cut(s) 842, 1187
MspI CCGG 4 cut(s) 326, 330, 495, 1050
Mva1269I GAATGC 1 cut(s) 953
MvnI CGCG 1 cut(s) 7
MwoI GCNNNNNNNGC 4 cut(s) 166, 446, 455, 541
NaeI GCCGGC 1 cut(s) 327
NcoI CCATGG 1 cut(s) 111
NdeI CATATG 1 cut(s) 321
NdeII GATC 9 cut(s) 81, 189, 478, 557, 658, 773, 783, 937, 1231
NgoMIV GCCGGC 1 cut(s) 325
NlaIII CATG 4 cut(s) 115, 180, 1134, 1260
NlaIV GGNNCC 4 cut(s) 785, 798, 1002, 1048
NmeAIII GCCGAG 1 cut(s) 179
NmuCI GTSAC 3 cut(s) 383, 680, 1259
NsiI ATGCAT 1 cut(s) 709
NspI RCATGY 2 cut(s) 180, 1260
PciI ACATGT 1 cut(s) 176
PctI GAATGC 1 cut(s) 953
PdiI GCCGGC 1 cut(s) 327
PfeI GAWTC 2 cut(s) 336, 410
PflMI CCANNNNNTGG 2 cut(s) 96, 497
PkrI GCNGC 6 cut(s) 6, 274, 457, 952, 1186, 1189
PleI GAGTC 4 cut(s) 239, 345, 384, 465
PpsI GAGTC 4 cut(s) 239, 345, 384, 465
Ppu21I YACGTR 1 cut(s) 41
PscI ACATGT 1 cut(s) 176
Psp124BI GAGCTC 1 cut(s) 1026
PspEI GGTNACC 2 cut(s) 383, 680
PspN4I GGNNCC 4 cut(s) 785, 798, 1002, 1048
PspPI GGNCC 1 cut(s) 797
PstI CTGCAG 1 cut(s) 457
PstNI CAGNNNCTG 2 cut(s) 260, 541
PsuI RGATCY 4 cut(s) 478, 783, 937, 1231
PvuII CAGCTG 2 cut(s) 842, 1187
RsaI GTAC 4 cut(s) 43, 119, 617, 1061
RsaNI GTAC 4 cut(s) 42, 118, 616, 1060
SacI GAGCTC 1 cut(s) 1026
SaqAI TTAA 2 cut(s) 599, 897
SatI GCNGC 6 cut(s) 5, 273, 456, 951, 1185, 1188
Sau3AI GATC 9 cut(s) 81, 189, 478, 557, 658, 773, 783, 937, 1231
Sau96I GGNCC 1 cut(s) 797
ScaI AGTACT 1 cut(s) 617
SchI GAGTC 4 cut(s) 239, 345, 385, 465
SduI GDGCHC 1 cut(s) 1026
SfaNI GCATC 3 cut(s) 284, 583, 716
SfcI CTRYAG 1 cut(s) 453
SnaBI TACGTA 1 cut(s) 41
Sse9I AATT 8 cut(s) 28, 250, 424, 442, 1095, 1146, 1193, 1209
SsiI CCGC 2 cut(s) 5, 273
SspI AATATT 1 cut(s) 588
SspMI CTAG 1 cut(s) 269
SstI GAGCTC 1 cut(s) 1026
StyI CCWWGG 2 cut(s) 111, 606
TaaI ACNGT 2 cut(s) 215, 1102
TaiI ACGT 1 cut(s) 43
TaqI TCGA 5 cut(s) 10, 80, 360, 577, 642
TasI AATT 8 cut(s) 28, 250, 424, 442, 1095, 1146, 1193, 1209
TatI WGTACW 2 cut(s) 615, 1059
TauI GCSGC 2 cut(s) 7, 275
TfiI GAWTC 2 cut(s) 336, 410
Tru1I TTAA 2 cut(s) 599, 897
Tru9I TTAA 2 cut(s) 599, 897
TscAI CASTG 2 cut(s) 733, 1081
TseFI GTSAC 3 cut(s) 383, 680, 1259
TseI GCWGC 4 cut(s) 455, 950, 1184, 1187
Tsp45I GTSAC 3 cut(s) 383, 680, 1259
TspDTI ATGAA 4 cut(s) 36, 894, 1119, 1160
TspRI CASTG 2 cut(s) 733, 1081
Van91I CCANNNNNTGG 2 cut(s) 96, 497
XapI RAATTY 5 cut(s) 250, 424, 442, 1193, 1209
XceI RCATGY 2 cut(s) 180, 1260
XcmI CCANNNNNNNNNTGG 2 cut(s) 659, 1121
XspI CTAG 1 cut(s) 269
ZrmI AGTACT 1 cut(s) 617
Zsp2I ATGCAT 1 cut(s) 709
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.