Rmu_sc0010663.1_g000005

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0010663.1
OG0000978
Physical Location & Seq
Forward (+)
14549 .. 16873
2325 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0010663.1_g000005.1.cds

Sequence Viewer

Length: 1167 bp
atggggtactctcctaaagttatcgtctctctgttctgctcctatatgcttatattgcttattacatgtagtgctgtgatccctgcctcggctttgaaagactgtgagtttcctgccatcttcaacttcggagactcaaattcagatactggtggactagccgcatcccttctaccaccaaccacaccttatggagagacctatttccatatgccggccggaagattctctgatggaagactcatcatcgatttccttgcaaagagtcttggtcacccttatctaagcgcatatctggattctctcgggacaaatttctcgtatggtgcaaattttgcaactgcagcttccactatcagactcccagatctcattataccagccggtggatttagtcccttctaccttgatattcaatatatgcagttcctgcaactaaaatccagatcacaactcataaggcatcgagggggaatatttgcaagtttaatgcccaaggaggagtacttctccaaagctttatacacattcgacattggccagaatgatcttggcgagggatttttcggtgacctgactatacaggaagtaaatgcatctgttcctgatataatcactggtttctcaactaatatcaagaaaatatatgatttgggagcaagatcattatggatccacaataccgggccgattggctgtctcccttacattgtggcaaacttcccgtcagctgagaaagatgagattggctgtgcaaaggcttacaatgaagtagctcaacattttaaccacgagctgaagcaggccatagttcaactcaggaaggatcttcctttggctgcattcacttatgtagatgtgtattctgtcaaatactctcttttcaaggaacccgaaaagtatggatttgagctcccacttgttgcttgttgtggctccggtggtaagtacaactataacgccactgctgaatgtggagcaataattacagtcaatggaagccaaatccttgttggttcatgcaaaaacccctcaattagagtgaactgggatggtgttcattacaccgaggcagctgcgaaatttgtttttgataaaatttcaactggagcgtattcagatccacctcttcccttgaaacaagcatgtcacaggagtttgaactaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005488 GO:0006950 GO:0006952 GO:0008150 GO:0016787 GO:0016788 GO:0019863 GO:0019865 GO:0044877 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

388

Amino Acids

42.46

Weight (kDa)

6.07

Isoelectric Point (pI)

38.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 386
AciI CCGC 1 cut(s) 162
AclWI GGATC 5 cut(s) 73, 667, 680, 834, 1115
AcoI YGGCCR 2 cut(s) 216, 538
AcsI RAATTY 5 cut(s) 139, 313, 331, 1082, 1098
AcuI CTGAAG 1 cut(s) 818
AfaI GTAC 3 cut(s) 8, 506, 950
AfiI CCNNNNNNNGG 3 cut(s) 88, 214, 386
AflIII ACRYGT 1 cut(s) 65
AgsI TTSAA 8 cut(s) 97, 124, 416, 815, 886, 1104, 1138, 1162
AluBI AGCT 7 cut(s) 347, 518, 731, 776, 796, 913, 1076
AluI AGCT 7 cut(s) 347, 518, 731, 776, 796, 913, 1076
Alw21I GWGCWC 1 cut(s) 915
Alw26I GTCTC 4 cut(s) 31, 126, 191, 704
AlwI GGATC 5 cut(s) 73, 667, 680, 834, 1115
AlwNI CAGNNNCTG 2 cut(s) 149, 430
Ama87I CYCGRG 1 cut(s) 305
AoxI GGCC 4 cut(s) 216, 538, 686, 804
ApeKI GCWGC 4 cut(s) 344, 839, 1073, 1076
ApoI RAATTY 5 cut(s) 139, 313, 331, 1082, 1098
ArsI GACNNNNNNTTYG 2 cut(s) 710, 742
AspLEI GCGC 1 cut(s) 290
AspS9I GGNCC 1 cut(s) 686
AsuC2I CCSGG 1 cut(s) 685
AsuHPI GGTGA 2 cut(s) 266, 581
AvaI CYCGRG 1 cut(s) 305
BalI TGGCCA 1 cut(s) 540
BamHI GGATCC 1 cut(s) 672
BanII GRGCYC 1 cut(s) 915
BauI CACGAG 1 cut(s) 791
BbsI GAAGAC 1 cut(s) 244
Bbv12I GWGCWC 1 cut(s) 915
BbvI GCAGC 4 cut(s) 356, 826, 1063, 1085
BccI CCATC 3 cut(s) 125, 227, 1046
BcgI CGANNNNNNTGC 4 cut(s) 239, 273, 1058, 1092
BcnI CCSGG 1 cut(s) 685
BcoDI GTCTC 4 cut(s) 31, 126, 191, 704
BfaI CTAG 1 cut(s) 158
BfmI CTRYAG 1 cut(s) 342
BglII AGATCT 1 cut(s) 367
BisI GCNGC 5 cut(s) 162, 345, 840, 1074, 1077
BlsI GCNGC 5 cut(s) 163, 346, 841, 1075, 1078
BmcAI AGTACT 1 cut(s) 506
Bme1390I CCNGG 1 cut(s) 685
BmeT110I CYCGRG 1 cut(s) 305
BmgT120I GGNCC 1 cut(s) 686
BmiI GGNNCC 3 cut(s) 674, 891, 937
BmrFI CCNGG 1 cut(s) 685
BmrI ACTGGG 1 cut(s) 1057
BmsI GCATC 3 cut(s) 173, 472, 605
BmuI ACTGGG 1 cut(s) 1057
BpiI GAAGAC 1 cut(s) 244
BplI GAGNNNNNCTC 2 cut(s) 494, 526
BpmI CTGGAG 1 cut(s) 1128
BpuMI CCSGG 1 cut(s) 685
Bsa29I ATCGAT 1 cut(s) 249
BsaI GGTCTC 1 cut(s) 191
BsaJI CCNNGG 3 cut(s) 87, 495, 1068
BsaWI WCCGGW 1 cut(s) 938
Bsc4I CCNNNNNNNGG 3 cut(s) 88, 214, 386
Bse118I RCCGGY 2 cut(s) 214, 383
Bse1I ACTGG 4 cut(s) 154, 622, 1052, 1111
BseCI ATCGAT 1 cut(s) 249
BseDI CCNNGG 3 cut(s) 87, 495, 1068
BseGI GGATG 2 cut(s) 164, 1057
BseLI CCNNNNNNNGG 3 cut(s) 88, 214, 386
BseMII CTCAG 2 cut(s) 723, 832
BseNI ACTGG 4 cut(s) 154, 622, 1052, 1111
BseRI GAGGAG 1 cut(s) 515
BseX3I CGGCCG 1 cut(s) 216
BseXI GCAGC 4 cut(s) 356, 826, 1063, 1085
Bsh1285I CGRYCG 1 cut(s) 219
BshFI GGCC 4 cut(s) 218, 540, 688, 806
BshVI ATCGAT 1 cut(s) 249
BsiEI CGRYCG 1 cut(s) 219
BsiHKAI GWGCWC 1 cut(s) 915
BsiHKCI CYCGRG 1 cut(s) 305
BsiSI CCGG 5 cut(s) 215, 219, 384, 684, 939
BslFI GGGAC 2 cut(s) 322, 381
BslI CCNNNNNNNGG 3 cut(s) 88, 214, 386
BsmAI GTCTC 4 cut(s) 31, 126, 191, 704
BsmBI CGTCTC 1 cut(s) 31
BsmFI GGGAC 2 cut(s) 322, 381
BsmI GAATGC 1 cut(s) 842
BsnI GGCC 4 cut(s) 218, 540, 688, 806
Bso31I GGTCTC 1 cut(s) 191
BsoBI CYCGRG 1 cut(s) 305
Bsp1286I GDGCHC 1 cut(s) 915
Bsp143I GATC 8 cut(s) 78, 367, 446, 547, 662, 672, 826, 1120
BspACI CCGC 1 cut(s) 162
BspANI GGCC 4 cut(s) 218, 540, 688, 806
BspCNI CTCAG 2 cut(s) 724, 831
BspDI ATCGAT 1 cut(s) 249
BspLI GGNNCC 3 cut(s) 674, 891, 937
BspMAI CTGCAG 1 cut(s) 346
BspPI GGATC 5 cut(s) 73, 667, 680, 834, 1115
BspTNI GGTCTC 1 cut(s) 191
BsrFI RCCGGY 2 cut(s) 214, 383
BsrI ACTGG 4 cut(s) 154, 622, 1052, 1111
BssAI RCCGGY 2 cut(s) 214, 383
BssECI CCNNGG 3 cut(s) 87, 495, 1068
BssMI GATC 8 cut(s) 78, 367, 446, 547, 662, 672, 826, 1120
BssSI CACGAG 1 cut(s) 791
BssT1I CCWWGG 1 cut(s) 495
Bst2BI CACGAG 1 cut(s) 791
Bst4CI ACNGT 2 cut(s) 104, 991
Bst6I CTCTTC 1 cut(s) 1134
BstAPI GCANNNNNTGC 2 cut(s) 335, 430
BstC8I GCNNGC 2 cut(s) 216, 804
BstDEI CTNAG 3 cut(s) 284, 732, 818
BstEII GGTNACC 2 cut(s) 272, 569
BstF5I GGATG 2 cut(s) 164, 1057
BstHHI GCGC 1 cut(s) 290
BstKTI GATC 8 cut(s) 81, 370, 449, 550, 665, 675, 829, 1123
BstMAI GTCTC 4 cut(s) 31, 126, 191, 704
BstMBI GATC 8 cut(s) 78, 367, 446, 547, 662, 672, 826, 1120
BstMCI CGRYCG 1 cut(s) 219
BstMWI GCNNNNNNNGC 4 cut(s) 55, 335, 344, 430
BstNSI RCATGY 2 cut(s) 69, 1149
BstPI GGTNACC 2 cut(s) 272, 569
BstSCI CCNGG 1 cut(s) 683
BstSFI CTRYAG 1 cut(s) 342
BstV1I GCAGC 4 cut(s) 356, 826, 1063, 1085
BstV2I GAAGAC 1 cut(s) 244
BstX2I RGATCY 4 cut(s) 367, 672, 826, 1120
BstYI RGATCY 4 cut(s) 367, 672, 826, 1120
BstZI CGGCCG 1 cut(s) 216
Bsu15I ATCGAT 1 cut(s) 249
BsuRI GGCC 4 cut(s) 218, 540, 688, 806
BsuTUI ATCGAT 1 cut(s) 249
BtsCI GGATG 2 cut(s) 164, 1057
BtsI GCAGTG 1 cut(s) 963
BtsIMutI CAGTG 2 cut(s) 615, 963
Cac8I GCNNGC 2 cut(s) 216, 804
CaiI CAGNNNCTG 2 cut(s) 149, 430
CfoI GCGC 1 cut(s) 290
Cfr10I RCCGGY 2 cut(s) 214, 383
Cfr13I GGNCC 1 cut(s) 686
ClaI ATCGAT 1 cut(s) 249
Csp6I GTAC 3 cut(s) 7, 505, 949
CviAII CATG 3 cut(s) 66, 1020, 1146
CviQI GTAC 3 cut(s) 7, 505, 949
DdeI CTNAG 3 cut(s) 284, 732, 818
DpnI GATC 8 cut(s) 80, 369, 448, 549, 664, 674, 828, 1122
DpnII GATC 8 cut(s) 78, 367, 446, 547, 662, 672, 826, 1120
EaeI YGGCCR 2 cut(s) 216, 538
EagI CGGCCG 1 cut(s) 216
Eam1104I CTCTTC 1 cut(s) 1134
EarI CTCTTC 1 cut(s) 1134
Ecl136II GAGCTC 1 cut(s) 913
EclXI CGGCCG 1 cut(s) 216
Eco130I CCWWGG 1 cut(s) 495
Eco24I GRGCYC 1 cut(s) 915
Eco31I GGTCTC 1 cut(s) 191
Eco52I CGGCCG 1 cut(s) 216
Eco53kI GAGCTC 1 cut(s) 913
Eco57I CTGAAG 1 cut(s) 818
Eco88I CYCGRG 1 cut(s) 305
Eco91I GGTNACC 2 cut(s) 272, 569
EcoICRI GAGCTC 1 cut(s) 913
EcoO65I GGTNACC 2 cut(s) 272, 569
EcoT14I CCWWGG 1 cut(s) 495
EcoT22I ATGCAT 1 cut(s) 598
EcoT38I GRGCYC 1 cut(s) 915
ErhI CCWWGG 1 cut(s) 495
Esp3I CGTCTC 1 cut(s) 31
FaeI CATG 3 cut(s) 69, 1023, 1149
FaqI GGGAC 2 cut(s) 322, 381
FatI CATG 3 cut(s) 65, 1019, 1145
FauNDI CATATG 1 cut(s) 210
Fnu4HI GCNGC 5 cut(s) 162, 345, 840, 1074, 1077
FokI GGATG 2 cut(s) 151, 1064
FriOI GRGCYC 1 cut(s) 915
Fsp4HI GCNGC 5 cut(s) 162, 345, 840, 1074, 1077
FspBI CTAG 1 cut(s) 158
GlaI GCGC 1 cut(s) 289
GluI GCNGC 5 cut(s) 162, 345, 840, 1074, 1077
GsuI CTGGAG 1 cut(s) 1128
HaeIII GGCC 4 cut(s) 218, 540, 688, 806
HapII CCGG 5 cut(s) 215, 219, 384, 684, 939
HhaI GCGC 1 cut(s) 290
Hin1II CATG 3 cut(s) 69, 1023, 1149
Hin6I GCGC 1 cut(s) 288
HinP1I GCGC 1 cut(s) 288
HindIII AAGCTT 1 cut(s) 516
HinfI GANTC 6 cut(s) 134, 225, 240, 265, 299, 360
HpaII CCGG 5 cut(s) 215, 219, 384, 684, 939
HphI GGTGA 2 cut(s) 266, 581
Hpy166II GTNNAC 2 cut(s) 155, 1045
Hpy188I TCNGA 5 cut(s) 131, 145, 232, 359, 1120
Hpy188III TCNNGA 6 cut(s) 296, 307, 444, 605, 637, 820
Hpy8I GTNNAC 2 cut(s) 155, 1045
HpyAV CCTTC 3 cut(s) 179, 409, 817
HpyCH4III ACNGT 2 cut(s) 104, 991
HpyF10VI GCNNNNNNNGC 4 cut(s) 55, 335, 344, 430
HpyF3I CTNAG 3 cut(s) 284, 732, 818
Hsp92II CATG 3 cut(s) 69, 1023, 1149
HspAI GCGC 1 cut(s) 288
KroI GCCGGC 1 cut(s) 214
KroNI GCCGGC 1 cut(s) 216
Kzo9I GATC 8 cut(s) 78, 367, 446, 547, 662, 672, 826, 1120
LmnI GCTCC 6 cut(s) 44, 656, 918, 941, 977, 1109
Lsp1109I GCAGC 4 cut(s) 356, 826, 1063, 1085
LweI GCATC 3 cut(s) 173, 472, 605
MaeI CTAG 1 cut(s) 158
MaeIII GTNAC 3 cut(s) 272, 569, 1148
MalI GATC 8 cut(s) 80, 369, 448, 549, 664, 674, 828, 1122
MboI GATC 8 cut(s) 78, 367, 446, 547, 662, 672, 826, 1120
MboII GAAGA 5 cut(s) 112, 234, 249, 821, 1121
MflI RGATCY 4 cut(s) 367, 672, 826, 1120
MhlI GDGCHC 1 cut(s) 915
MlsI TGGCCA 1 cut(s) 540
MluCI AATT 7 cut(s) 139, 313, 331, 984, 1035, 1082, 1098
MluNI TGGCCA 1 cut(s) 540
MlyI GAGTC 4 cut(s) 128, 234, 274, 354
MnlI CCTC 7 cut(s) 97, 461, 493, 550, 1042, 1063, 1137
Mox20I TGGCCA 1 cut(s) 540
Mph1103I ATGCAT 1 cut(s) 598
MroNI GCCGGC 1 cut(s) 214
MscI TGGCCA 1 cut(s) 540
MseI TTAA 2 cut(s) 488, 786
Msp20I TGGCCA 1 cut(s) 540
MspA1I CMGCKG 2 cut(s) 731, 1076
MspI CCGG 5 cut(s) 215, 219, 384, 684, 939
MspR9I CCNGG 1 cut(s) 685
Mva1269I GAATGC 1 cut(s) 842
MwoI GCNNNNNNNGC 4 cut(s) 55, 335, 344, 430
NaeI GCCGGC 1 cut(s) 216
NciI CCSGG 1 cut(s) 685
NdeI CATATG 1 cut(s) 210
NdeII GATC 8 cut(s) 78, 367, 446, 547, 662, 672, 826, 1120
NgoMIV GCCGGC 1 cut(s) 214
NlaIII CATG 3 cut(s) 69, 1023, 1149
NlaIV GGNNCC 3 cut(s) 674, 891, 937
NmeAIII GCCGAG 1 cut(s) 68
NmuCI GTSAC 3 cut(s) 272, 569, 1148
NsiI ATGCAT 1 cut(s) 598
NspI RCATGY 2 cut(s) 69, 1149
PciI ACATGT 1 cut(s) 65
PctI GAATGC 1 cut(s) 842
PdiI GCCGGC 1 cut(s) 216
PfeI GAWTC 2 cut(s) 225, 299
PflMI CCANNNNNTGG 1 cut(s) 386
PkrI GCNGC 5 cut(s) 163, 346, 841, 1075, 1078
PleI GAGTC 4 cut(s) 128, 234, 273, 354
PpsI GAGTC 4 cut(s) 128, 234, 273, 354
PscI ACATGT 1 cut(s) 65
Psp124BI GAGCTC 1 cut(s) 915
PspEI GGTNACC 2 cut(s) 272, 569
PspN4I GGNNCC 3 cut(s) 674, 891, 937
PspPI GGNCC 1 cut(s) 686
PstI CTGCAG 1 cut(s) 346
PstNI CAGNNNCTG 2 cut(s) 149, 430
PsuI RGATCY 4 cut(s) 367, 672, 826, 1120
PvuII CAGCTG 2 cut(s) 731, 1076
RsaI GTAC 3 cut(s) 8, 506, 950
RsaNI GTAC 3 cut(s) 7, 505, 949
SacI GAGCTC 1 cut(s) 915
SaqAI TTAA 2 cut(s) 488, 786
SatI GCNGC 5 cut(s) 162, 345, 840, 1074, 1077
Sau3AI GATC 8 cut(s) 78, 367, 446, 547, 662, 672, 826, 1120
Sau96I GGNCC 1 cut(s) 686
ScaI AGTACT 1 cut(s) 506
SchI GAGTC 4 cut(s) 128, 234, 274, 354
ScrFI CCNGG 1 cut(s) 685
SduI GDGCHC 1 cut(s) 915
SfaNI GCATC 3 cut(s) 173, 472, 605
SfcI CTRYAG 1 cut(s) 342
Sse9I AATT 7 cut(s) 139, 313, 331, 984, 1035, 1082, 1098
SsiI CCGC 1 cut(s) 162
SspI AATATT 1 cut(s) 477
SspMI CTAG 1 cut(s) 158
SstI GAGCTC 1 cut(s) 915
StyD4I CCNGG 1 cut(s) 683
StyI CCWWGG 1 cut(s) 495
TaaI ACNGT 2 cut(s) 104, 991
TaqI TCGA 3 cut(s) 249, 466, 531
TasI AATT 7 cut(s) 139, 313, 331, 984, 1035, 1082, 1098
TatI WGTACW 2 cut(s) 504, 948
TauI GCSGC 1 cut(s) 164
TfiI GAWTC 2 cut(s) 225, 299
Tru1I TTAA 2 cut(s) 488, 786
Tru9I TTAA 2 cut(s) 488, 786
TscAI CASTG 2 cut(s) 622, 970
TseFI GTSAC 3 cut(s) 272, 569, 1148
TseI GCWGC 4 cut(s) 344, 839, 1073, 1076
Tsp45I GTSAC 3 cut(s) 272, 569, 1148
TspDTI ATGAA 3 cut(s) 783, 1008, 1049
TspRI CASTG 2 cut(s) 622, 970
Van91I CCANNNNNTGG 1 cut(s) 386
XapI RAATTY 5 cut(s) 139, 313, 331, 1082, 1098
XceI RCATGY 2 cut(s) 69, 1149
XcmI CCANNNNNNNNNTGG 2 cut(s) 548, 1010
XspI CTAG 1 cut(s) 158
ZrmI AGTACT 1 cut(s) 506
Zsp2I ATGCAT 1 cut(s) 598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.