Rw6G032080

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000978
Physical Location & Seq
Reverse (-)
56200282 .. 56202624
2343 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G032080.1

Sequence Viewer

Length: 1170 bp
ATGGGGTACTCTCCTAAAGTTATTATTTCTCTGTTCTGCTCCTACATGCTTATATTGCATATTACCAGTAGTTCTGTTATCCCTACCTTGGCTTTGAAAGACTGTGAGTTTCCTGCCATCTTCAACTTCGGAGACTCAAATTCAGATACTGGTGGACTAGCCGCATCCCTTCTATTAACACCAACCACACCTTATGGAGAGACCTATTTCCATATGCCGGCCGGAAGATTCTCTGATGGAAGACTCATCATCGATTTCCTAGCAAAGAGTCTTGGTCACCCTTATCTAAGCGCATATCTGGATTCTCTCGGGACAAATTTCTCGCATGGTGCTAATTTCGCCACTGCAGCTTCCACTATCAGACTCCCAGATCTCATTTTACCAGCCGGTCAATTTAGTCCCTTCTACCTTGATATTCAATATATGCAGTTCCTGCAACTAAAATCCAGATCACAACTCATAAGGCATCGAGGGGGAATATTTGCAAGTTTAATGCCCAAGGAGGAGTACTTCTCCAAAGCTTTATACACATTCGACATTGGTCAGAATGATCTTGGCGAGGGATTTTTCGGTAACATGACTATACAGGAAGTAAATGCATCTGTTCCTGATATAATCACTGGTTTCTCAACTAATATCAAGAAAATATATGATTTGGGAGCAAGATCATTATGGATCCACAATACGGGGCCGATTGGCTGTCTCCCTTACATTGTGGCAGACTTCCCGTCAGCTGAGAAAGATGAGATTGGCTGTGCAAAGGCTTACAATGAAGTAGCTCAACATTTTAACCACGAGCTGAAGCAGGCCATAGTTCAACTCAGGAAGGATCTTCCTTTGGCTGCATTCACTTATGTAGATGTGTATTCTGTCAAATACTCTCTTTTCAAGGAACCTGAAAAGTATGGATTTGAGCTCCCACTTGTTGCTTGTTGTGGCTCCGGTGGTAAGTACAACTATAACGCCACTGCTGGATGTGGAGCAATAATTACAGTCAATGGAAGCCAAATCCTTGTTGGTTCATGCAAAAACCCCTCAATTAGAGTGAACTGGGATGGTGTTCATTACACCGAGGCAGCTGCGAAATTTGTTTTTGATAAAATTTCAACTGGAGCGTATTCAGATCCACCTCTTCCCTTGAAACAAGCATGTCACAGGAGTTTGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005488 GO:0006950 GO:0006952 GO:0008150 GO:0016787 GO:0016788 GO:0019863 GO:0019865 GO:0044877 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

389

Amino Acids

42.66

Weight (kDa)

6.36

Isoelectric Point (pI)

38.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 40 - 368 1.6e-47 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 162
AclWI GGATC 4 cut(s) 670, 683, 837, 1118
AcoI YGGCCR 1 cut(s) 219
AcsI RAATTY 4 cut(s) 139, 316, 1085, 1101
AcuI CTGAAG 1 cut(s) 821
AfaI GTAC 3 cut(s) 8, 509, 953
AfiI CCNNNNNNNGG 3 cut(s) 88, 217, 685
AgsI TTSAA 8 cut(s) 97, 124, 419, 818, 889, 1107, 1141, 1165
AhdI GACNNNNNGTC 1 cut(s) 727
AluBI AGCT 7 cut(s) 350, 521, 734, 779, 799, 916, 1079
AluI AGCT 7 cut(s) 350, 521, 734, 779, 799, 916, 1079
Alw21I GWGCWC 1 cut(s) 918
Alw26I GTCTC 3 cut(s) 126, 194, 707
AlwI GGATC 4 cut(s) 670, 683, 837, 1118
AlwNI CAGNNNCTG 2 cut(s) 149, 433
Ama87I CYCGRG 1 cut(s) 308
AoxI GGCC 3 cut(s) 219, 689, 807
ApeKI GCWGC 4 cut(s) 347, 842, 1076, 1079
ApoI RAATTY 4 cut(s) 139, 316, 1085, 1101
AspLEI GCGC 1 cut(s) 293
AspS9I GGNCC 1 cut(s) 689
AsuHPI GGTGA 1 cut(s) 269
AvaI CYCGRG 1 cut(s) 308
BamHI GGATCC 1 cut(s) 675
BanII GRGCYC 1 cut(s) 918
BauI CACGAG 1 cut(s) 794
BbsI GAAGAC 1 cut(s) 247
Bbv12I GWGCWC 1 cut(s) 918
BbvI GCAGC 4 cut(s) 359, 829, 1066, 1088
BccI CCATC 3 cut(s) 125, 230, 1049
BcgI CGANNNNNNTGC 2 cut(s) 1061, 1095
BcoDI GTCTC 3 cut(s) 126, 194, 707
BfaI CTAG 2 cut(s) 158, 260
BfmI CTRYAG 1 cut(s) 345
BglII AGATCT 1 cut(s) 370
BisI GCNGC 5 cut(s) 162, 348, 843, 1077, 1080
BlsI GCNGC 5 cut(s) 163, 349, 844, 1078, 1081
BmcAI AGTACT 1 cut(s) 509
BmeRI GACNNNNNGTC 1 cut(s) 727
BmeT110I CYCGRG 1 cut(s) 308
BmgT120I GGNCC 1 cut(s) 689
BmiI GGNNCC 4 cut(s) 677, 690, 894, 940
BmrI ACTGGG 1 cut(s) 1060
BmsI GCATC 3 cut(s) 173, 475, 608
BmuI ACTGGG 1 cut(s) 1060
BoxI GACNNNNGTC 1 cut(s) 540
BpiI GAAGAC 1 cut(s) 247
BplI GAGNNNNNCTC 2 cut(s) 497, 529
BpmI CTGGAG 1 cut(s) 1131
Bsa29I ATCGAT 1 cut(s) 252
BsaI GGTCTC 1 cut(s) 194
BsaJI CCNNGG 3 cut(s) 87, 498, 1071
BsaWI WCCGGW 1 cut(s) 941
Bsc4I CCNNNNNNNGG 3 cut(s) 88, 217, 685
Bse118I RCCGGY 2 cut(s) 217, 386
Bse1I ACTGG 5 cut(s) 66, 154, 625, 1055, 1114
BseCI ATCGAT 1 cut(s) 252
BseDI CCNNGG 3 cut(s) 87, 498, 1071
BseGI GGATG 3 cut(s) 164, 980, 1060
BseLI CCNNNNNNNGG 3 cut(s) 88, 217, 685
BseMII CTCAG 2 cut(s) 726, 835
BseNI ACTGG 5 cut(s) 66, 154, 625, 1055, 1114
BseRI GAGGAG 1 cut(s) 518
BseX3I CGGCCG 1 cut(s) 219
BseXI GCAGC 4 cut(s) 359, 829, 1066, 1088
Bsh1285I CGRYCG 1 cut(s) 222
BshFI GGCC 3 cut(s) 221, 691, 809
BshVI ATCGAT 1 cut(s) 252
BsiEI CGRYCG 1 cut(s) 222
BsiHKAI GWGCWC 1 cut(s) 918
BsiHKCI CYCGRG 1 cut(s) 308
BsiSI CCGG 4 cut(s) 218, 222, 387, 942
BslFI GGGAC 2 cut(s) 325, 384
BslI CCNNNNNNNGG 3 cut(s) 88, 217, 685
BsmAI GTCTC 3 cut(s) 126, 194, 707
BsmFI GGGAC 2 cut(s) 325, 384
BsmI GAATGC 1 cut(s) 845
BsnI GGCC 3 cut(s) 221, 691, 809
Bso31I GGTCTC 1 cut(s) 194
BsoBI CYCGRG 1 cut(s) 308
Bsp1286I GDGCHC 1 cut(s) 918
Bsp143I GATC 7 cut(s) 370, 449, 550, 665, 675, 829, 1123
BspACI CCGC 1 cut(s) 162
BspANI GGCC 3 cut(s) 221, 691, 809
BspCNI CTCAG 2 cut(s) 727, 834
BspDI ATCGAT 1 cut(s) 252
BspLI GGNNCC 4 cut(s) 677, 690, 894, 940
BspMAI CTGCAG 1 cut(s) 349
BspPI GGATC 4 cut(s) 670, 683, 837, 1118
BspTNI GGTCTC 1 cut(s) 194
BsrFI RCCGGY 2 cut(s) 217, 386
BsrI ACTGG 5 cut(s) 66, 154, 625, 1055, 1114
BssAI RCCGGY 2 cut(s) 217, 386
BssECI CCNNGG 3 cut(s) 87, 498, 1071
BssMI GATC 7 cut(s) 370, 449, 550, 665, 675, 829, 1123
BssSI CACGAG 1 cut(s) 794
BssT1I CCWWGG 2 cut(s) 87, 498
Bst2BI CACGAG 1 cut(s) 794
Bst4CI ACNGT 2 cut(s) 104, 994
Bst6I CTCTTC 1 cut(s) 1137
BstAPI GCANNNNNTGC 1 cut(s) 433
BstC8I GCNNGC 2 cut(s) 219, 807
BstDEI CTNAG 3 cut(s) 287, 735, 821
BstEII GGTNACC 1 cut(s) 275
BstF5I GGATG 3 cut(s) 164, 980, 1060
BstHHI GCGC 1 cut(s) 293
BstKTI GATC 7 cut(s) 373, 452, 553, 668, 678, 832, 1126
BstMAI GTCTC 3 cut(s) 126, 194, 707
BstMBI GATC 7 cut(s) 370, 449, 550, 665, 675, 829, 1123
BstMCI CGRYCG 1 cut(s) 222
BstMWI GCNNNNNNNGC 4 cut(s) 55, 338, 347, 433
BstNSI RCATGY 2 cut(s) 49, 1152
BstPAI GACNNNNGTC 1 cut(s) 540
BstPI GGTNACC 1 cut(s) 275
BstSFI CTRYAG 1 cut(s) 345
BstV1I GCAGC 4 cut(s) 359, 829, 1066, 1088
BstV2I GAAGAC 1 cut(s) 247
BstX2I RGATCY 4 cut(s) 370, 675, 829, 1123
BstYI RGATCY 4 cut(s) 370, 675, 829, 1123
BstZI CGGCCG 1 cut(s) 219
Bsu15I ATCGAT 1 cut(s) 252
BsuRI GGCC 3 cut(s) 221, 691, 809
BsuTUI ATCGAT 1 cut(s) 252
BtsCI GGATG 3 cut(s) 164, 980, 1060
BtsI GCAGTG 2 cut(s) 342, 966
BtsIMutI CAGTG 3 cut(s) 342, 618, 966
Cac8I GCNNGC 2 cut(s) 219, 807
CaiI CAGNNNCTG 2 cut(s) 149, 433
CfoI GCGC 1 cut(s) 293
Cfr10I RCCGGY 2 cut(s) 217, 386
Cfr13I GGNCC 1 cut(s) 689
ClaI ATCGAT 1 cut(s) 252
Csp6I GTAC 3 cut(s) 7, 508, 952
CviAII CATG 5 cut(s) 46, 326, 577, 1023, 1149
CviQI GTAC 3 cut(s) 7, 508, 952
DdeI CTNAG 3 cut(s) 287, 735, 821
DpnI GATC 7 cut(s) 372, 451, 552, 667, 677, 831, 1125
DpnII GATC 7 cut(s) 370, 449, 550, 665, 675, 829, 1123
DriI GACNNNNNGTC 1 cut(s) 727
EaeI YGGCCR 1 cut(s) 219
EagI CGGCCG 1 cut(s) 219
Eam1104I CTCTTC 1 cut(s) 1137
Eam1105I GACNNNNNGTC 1 cut(s) 727
EarI CTCTTC 1 cut(s) 1137
Ecl136II GAGCTC 1 cut(s) 916
EclXI CGGCCG 1 cut(s) 219
Eco130I CCWWGG 2 cut(s) 87, 498
Eco24I GRGCYC 1 cut(s) 918
Eco31I GGTCTC 1 cut(s) 194
Eco52I CGGCCG 1 cut(s) 219
Eco53kI GAGCTC 1 cut(s) 916
Eco57I CTGAAG 1 cut(s) 821
Eco88I CYCGRG 1 cut(s) 308
Eco91I GGTNACC 1 cut(s) 275
EcoICRI GAGCTC 1 cut(s) 916
EcoO65I GGTNACC 1 cut(s) 275
EcoT14I CCWWGG 2 cut(s) 87, 498
EcoT22I ATGCAT 1 cut(s) 601
EcoT38I GRGCYC 1 cut(s) 918
ErhI CCWWGG 2 cut(s) 87, 498
FaeI CATG 5 cut(s) 49, 329, 580, 1026, 1152
FaqI GGGAC 2 cut(s) 325, 384
FatI CATG 5 cut(s) 45, 325, 576, 1022, 1148
FauNDI CATATG 1 cut(s) 213
Fnu4HI GCNGC 5 cut(s) 162, 348, 843, 1077, 1080
FokI GGATG 3 cut(s) 151, 987, 1067
FriOI GRGCYC 1 cut(s) 918
Fsp4HI GCNGC 5 cut(s) 162, 348, 843, 1077, 1080
FspBI CTAG 2 cut(s) 158, 260
GlaI GCGC 1 cut(s) 292
GluI GCNGC 5 cut(s) 162, 348, 843, 1077, 1080
GsuI CTGGAG 1 cut(s) 1131
HaeIII GGCC 3 cut(s) 221, 691, 809
HapII CCGG 4 cut(s) 218, 222, 387, 942
HhaI GCGC 1 cut(s) 293
Hin1II CATG 5 cut(s) 49, 329, 580, 1026, 1152
Hin6I GCGC 1 cut(s) 291
HinP1I GCGC 1 cut(s) 291
HindIII AAGCTT 1 cut(s) 519
HinfI GANTC 6 cut(s) 134, 228, 243, 268, 302, 363
HpaII CCGG 4 cut(s) 218, 222, 387, 942
HphI GGTGA 1 cut(s) 269
Hpy166II GTNNAC 2 cut(s) 155, 1048
Hpy188I TCNGA 6 cut(s) 131, 145, 235, 362, 546, 1123
Hpy188III TCNNGA 6 cut(s) 299, 310, 447, 608, 640, 823
Hpy8I GTNNAC 2 cut(s) 155, 1048
HpyAV CCTTC 3 cut(s) 179, 412, 820
HpyCH4III ACNGT 2 cut(s) 104, 994
HpyCH4V TGCA 9 cut(s) 58, 347, 427, 436, 485, 599, 758, 845, 1026
HpyF10VI GCNNNNNNNGC 4 cut(s) 55, 338, 347, 433
HpyF3I CTNAG 3 cut(s) 287, 735, 821
Hsp92II CATG 5 cut(s) 49, 329, 580, 1026, 1152
HspAI GCGC 1 cut(s) 291
KroI GCCGGC 1 cut(s) 217
KroNI GCCGGC 1 cut(s) 219
Kzo9I GATC 7 cut(s) 370, 449, 550, 665, 675, 829, 1123
LmnI GCTCC 6 cut(s) 44, 659, 921, 944, 980, 1112
Lsp1109I GCAGC 4 cut(s) 359, 829, 1066, 1088
LweI GCATC 3 cut(s) 173, 475, 608
MaeI CTAG 2 cut(s) 158, 260
MaeIII GTNAC 3 cut(s) 275, 572, 1151
MalI GATC 7 cut(s) 372, 451, 552, 667, 677, 831, 1125
MboI GATC 7 cut(s) 370, 449, 550, 665, 675, 829, 1123
MboII GAAGA 5 cut(s) 112, 237, 252, 824, 1124
MflI RGATCY 4 cut(s) 370, 675, 829, 1123
MhlI GDGCHC 1 cut(s) 918
MluCI AATT 8 cut(s) 139, 316, 334, 392, 987, 1038, 1085, 1101
MlyI GAGTC 4 cut(s) 128, 237, 277, 357
MnlI CCTC 6 cut(s) 464, 496, 553, 1045, 1066, 1140
Mph1103I ATGCAT 1 cut(s) 601
MroNI GCCGGC 1 cut(s) 217
MseI TTAA 3 cut(s) 176, 491, 789
MspA1I CMGCKG 2 cut(s) 734, 1079
MspI CCGG 4 cut(s) 218, 222, 387, 942
Mva1269I GAATGC 1 cut(s) 845
MwoI GCNNNNNNNGC 4 cut(s) 55, 338, 347, 433
NaeI GCCGGC 1 cut(s) 219
NdeI CATATG 1 cut(s) 213
NdeII GATC 7 cut(s) 370, 449, 550, 665, 675, 829, 1123
NgoMIV GCCGGC 1 cut(s) 217
NlaIII CATG 5 cut(s) 49, 329, 580, 1026, 1152
NlaIV GGNNCC 4 cut(s) 677, 690, 894, 940
NmuCI GTSAC 2 cut(s) 275, 1151
NsiI ATGCAT 1 cut(s) 601
NspI RCATGY 2 cut(s) 49, 1152
PctI GAATGC 1 cut(s) 845
PdiI GCCGGC 1 cut(s) 219
PfeI GAWTC 2 cut(s) 228, 302
PkrI GCNGC 5 cut(s) 163, 349, 844, 1078, 1081
PleI GAGTC 4 cut(s) 128, 237, 276, 357
PpsI GAGTC 4 cut(s) 128, 237, 276, 357
PshAI GACNNNNGTC 1 cut(s) 540
Psp124BI GAGCTC 1 cut(s) 918
PspEI GGTNACC 1 cut(s) 275
PspN4I GGNNCC 4 cut(s) 677, 690, 894, 940
PspPI GGNCC 1 cut(s) 689
PstI CTGCAG 1 cut(s) 349
PstNI CAGNNNCTG 2 cut(s) 149, 433
PsuI RGATCY 4 cut(s) 370, 675, 829, 1123
PvuII CAGCTG 2 cut(s) 734, 1079
RsaI GTAC 3 cut(s) 8, 509, 953
RsaNI GTAC 3 cut(s) 7, 508, 952
SacI GAGCTC 1 cut(s) 918
SaqAI TTAA 3 cut(s) 176, 491, 789
SatI GCNGC 5 cut(s) 162, 348, 843, 1077, 1080
Sau3AI GATC 7 cut(s) 370, 449, 550, 665, 675, 829, 1123
Sau96I GGNCC 1 cut(s) 689
ScaI AGTACT 1 cut(s) 509
SchI GAGTC 4 cut(s) 128, 237, 277, 357
SduI GDGCHC 1 cut(s) 918
SfaNI GCATC 3 cut(s) 173, 475, 608
SfcI CTRYAG 1 cut(s) 345
Sse9I AATT 8 cut(s) 139, 316, 334, 392, 987, 1038, 1085, 1101
SsiI CCGC 1 cut(s) 162
SspI AATATT 1 cut(s) 480
SspMI CTAG 2 cut(s) 158, 260
SstI GAGCTC 1 cut(s) 918
StyI CCWWGG 2 cut(s) 87, 498
TaaI ACNGT 2 cut(s) 104, 994
TaqI TCGA 3 cut(s) 252, 469, 534
TasI AATT 8 cut(s) 139, 316, 334, 392, 987, 1038, 1085, 1101
TatI WGTACW 2 cut(s) 507, 951
TauI GCSGC 1 cut(s) 164
TfiI GAWTC 2 cut(s) 228, 302
Tru1I TTAA 3 cut(s) 176, 491, 789
Tru9I TTAA 3 cut(s) 176, 491, 789
TscAI CASTG 3 cut(s) 349, 625, 973
TseFI GTSAC 2 cut(s) 275, 1151
TseI GCWGC 4 cut(s) 347, 842, 1076, 1079
Tsp45I GTSAC 2 cut(s) 275, 1151
TspDTI ATGAA 3 cut(s) 786, 1011, 1052
TspRI CASTG 3 cut(s) 349, 625, 973
XapI RAATTY 4 cut(s) 139, 316, 1085, 1101
XceI RCATGY 2 cut(s) 49, 1152
XcmI CCANNNNNNNNNTGG 1 cut(s) 1013
XspI CTAG 2 cut(s) 158, 260
ZrmI AGTACT 1 cut(s) 509
Zsp2I ATGCAT 1 cut(s) 601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.