Rroxscaffold_7G00172160

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000978
Physical Location & Seq
Forward (+)
12427207 .. 12443012
15806 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00172160.1

Sequence Viewer

Length: 1173 bp
ATGGGGTACTCTCCTAAAGTTATCATTTCTCTGTTCTGCTCCTATATGCTTATATTGCATATTACCAGCAGTAGTGCTGTGATCCCTGCCTTGGCTTTGAAAGACTGTGAGTTTCCTGCCATCTTCAACTTCGGAGACTCAAATTCAGATACTGGTGGACTAGCCGCATCCCTTCTATCAACACCAACCCCACCTTATGGAGAGACCTATTTCCATATGCCGGCCGGAAGATTCTCTGATGGAAGACTCATCATCGATTTCCTTGCAAAGAGTCTTGGTCACCCTTATCTAAGCGCATATCTGGATTCTCTCGGGACAAATTTCTCGCATGGTGCAAATTTCGCCACTGCAGCTTCCACTATCAGACTCCCAGATCTCATTATACCAGCGGGTGGATTTAGTCCCTTCTACCTTGATATTCAATATATGCAGTTCCTGCAACTAAAATCCAGATCACAACTCATAAGGCATCAAGGGGGAATATTTGCAAGTTTAATGCCCAAGGAGGAGTACTTCTCCAAAGCTTTATACACATTCGACATTGGTCAGAATGATCTTGGCGAGGGATTTTTCGGTAACATGACTATACAAGAAGTCAATGCATCTGTTCCTGATATAATCACTGGTTTCTCAACTAATATCAAGAAAATATATGATTTGGGAGCAAGATCATTATGGATCCACAATACGGGGCCGATTGGCTGTCTCCCTTACATTGTGGCAAACTTCCCGTCAGCTGAGAAAGATGAGATTGGCTGTGCAAAGGCCTACAATGAAGTAGCTCAACATTTTAACCACGAGCTGAAGCAGGCCATAGTTCAACTCAGGAAGGATCTTCCTTTGGCTGCATTCACTTATGTAGATATGTATGCTGTCAAATACTCTCTTTTCAAGGAACCCGAAAAGTATGGATTTGAGCTCCCACTTGTTGCTTGTTGTGGCTCCGGTGGTAAGTACAACTATAACGCCACTGCTGGATGTGGAGCAATAATTACAGTCAATGGAAGCCAAGTCCTTGTTGGTTCATGCAAAAACCCCTCAACTAGAGTGAACTGGGATGGTGTTCATTACACCGAGGCAGCTGCAAAATTTATTTTTGATAGAATTTCAACTGGAGCGTATTCAGATCCACCTCTTCCCTTGAAACAAGCATGTCACAGGAGTTTGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005488 GO:0006950 GO:0006952 GO:0008150 GO:0016787 GO:0016788 GO:0019863 GO:0019865 GO:0044877 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

390

Amino Acids

42.61

Weight (kDa)

6.36

Isoelectric Point (pI)

39.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 41 - 369 1.2e-47 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 197, 392
AciI CCGC 2 cut(s) 165, 389
AclWI GGATC 5 cut(s) 76, 673, 686, 840, 1121
AcoI YGGCCR 1 cut(s) 222
AcsI RAATTY 5 cut(s) 142, 319, 337, 1088, 1104
AcuI CTGAAG 1 cut(s) 824
AfaI GTAC 3 cut(s) 8, 512, 956
AfiI CCNNNNNNNGG 5 cut(s) 91, 197, 220, 392, 688
AgsI TTSAA 8 cut(s) 100, 127, 422, 821, 892, 1110, 1144, 1168
AluBI AGCT 7 cut(s) 353, 524, 737, 782, 802, 919, 1082
AluI AGCT 7 cut(s) 353, 524, 737, 782, 802, 919, 1082
Alw21I GWGCWC 1 cut(s) 921
Alw26I GTCTC 3 cut(s) 129, 197, 710
AlwI GGATC 5 cut(s) 76, 673, 686, 840, 1121
AlwNI CAGNNNCTG 2 cut(s) 152, 436
Ama87I CYCGRG 1 cut(s) 311
AoxI GGCC 4 cut(s) 222, 692, 765, 810
ApeKI GCWGC 4 cut(s) 350, 845, 1079, 1082
ApoI RAATTY 5 cut(s) 142, 319, 337, 1088, 1104
ArsI GACNNNNNNTTYG 2 cut(s) 716, 748
AspLEI GCGC 1 cut(s) 296
AspS9I GGNCC 1 cut(s) 692
AsuHPI GGTGA 1 cut(s) 272
AvaI CYCGRG 1 cut(s) 311
BamHI GGATCC 1 cut(s) 678
BanII GRGCYC 1 cut(s) 921
BauI CACGAG 1 cut(s) 797
BbsI GAAGAC 1 cut(s) 250
Bbv12I GWGCWC 1 cut(s) 921
BbvI GCAGC 4 cut(s) 362, 832, 1069, 1091
BccI CCATC 3 cut(s) 128, 233, 1052
BcgI CGANNNNNNTGC 4 cut(s) 245, 279, 1064, 1098
BcoDI GTCTC 3 cut(s) 129, 197, 710
BfaI CTAG 2 cut(s) 161, 1044
BfmI CTRYAG 1 cut(s) 348
BglII AGATCT 1 cut(s) 373
BisI GCNGC 5 cut(s) 165, 351, 846, 1080, 1083
BlsI GCNGC 5 cut(s) 166, 352, 847, 1081, 1084
BmcAI AGTACT 1 cut(s) 512
BmeT110I CYCGRG 1 cut(s) 311
BmgT120I GGNCC 1 cut(s) 692
BmiI GGNNCC 4 cut(s) 680, 693, 897, 943
BmrI ACTGGG 1 cut(s) 1063
BmsI GCATC 3 cut(s) 176, 478, 611
BmuI ACTGGG 1 cut(s) 1063
BoxI GACNNNNGTC 1 cut(s) 543
BpiI GAAGAC 1 cut(s) 250
BplI GAGNNNNNCTC 2 cut(s) 500, 532
BpmI CTGGAG 1 cut(s) 1134
Bsa29I ATCGAT 1 cut(s) 255
BsaI GGTCTC 1 cut(s) 197
BsaJI CCNNGG 3 cut(s) 90, 501, 1074
BsaWI WCCGGW 1 cut(s) 944
Bsc4I CCNNNNNNNGG 5 cut(s) 91, 197, 220, 392, 688
Bse118I RCCGGY 1 cut(s) 220
Bse1I ACTGG 4 cut(s) 157, 628, 1058, 1117
BseCI ATCGAT 1 cut(s) 255
BseDI CCNNGG 3 cut(s) 90, 501, 1074
BseGI GGATG 3 cut(s) 167, 983, 1063
BseLI CCNNNNNNNGG 5 cut(s) 91, 197, 220, 392, 688
BseMII CTCAG 2 cut(s) 729, 838
BseNI ACTGG 4 cut(s) 157, 628, 1058, 1117
BseRI GAGGAG 1 cut(s) 521
BseX3I CGGCCG 1 cut(s) 222
BseXI GCAGC 4 cut(s) 362, 832, 1069, 1091
Bsh1285I CGRYCG 1 cut(s) 225
BshFI GGCC 4 cut(s) 224, 694, 767, 812
BshVI ATCGAT 1 cut(s) 255
BsiEI CGRYCG 1 cut(s) 225
BsiHKAI GWGCWC 1 cut(s) 921
BsiHKCI CYCGRG 1 cut(s) 311
BsiSI CCGG 3 cut(s) 221, 225, 945
BslFI GGGAC 2 cut(s) 328, 387
BslI CCNNNNNNNGG 5 cut(s) 91, 197, 220, 392, 688
BsmAI GTCTC 3 cut(s) 129, 197, 710
BsmFI GGGAC 2 cut(s) 328, 387
BsmI GAATGC 1 cut(s) 848
BsnI GGCC 4 cut(s) 224, 694, 767, 812
Bso31I GGTCTC 1 cut(s) 197
BsoBI CYCGRG 1 cut(s) 311
Bsp1286I GDGCHC 1 cut(s) 921
Bsp143I GATC 8 cut(s) 81, 373, 452, 553, 668, 678, 832, 1126
BspACI CCGC 2 cut(s) 165, 389
BspANI GGCC 4 cut(s) 224, 694, 767, 812
BspCNI CTCAG 2 cut(s) 730, 837
BspDI ATCGAT 1 cut(s) 255
BspLI GGNNCC 4 cut(s) 680, 693, 897, 943
BspMAI CTGCAG 1 cut(s) 352
BspPI GGATC 5 cut(s) 76, 673, 686, 840, 1121
BspTNI GGTCTC 1 cut(s) 197
BsrFI RCCGGY 1 cut(s) 220
BsrI ACTGG 4 cut(s) 157, 628, 1058, 1117
BssAI RCCGGY 1 cut(s) 220
BssECI CCNNGG 3 cut(s) 90, 501, 1074
BssMI GATC 8 cut(s) 81, 373, 452, 553, 668, 678, 832, 1126
BssSI CACGAG 1 cut(s) 797
BssT1I CCWWGG 2 cut(s) 90, 501
Bst2BI CACGAG 1 cut(s) 797
Bst4CI ACNGT 2 cut(s) 107, 997
Bst6I CTCTTC 1 cut(s) 1140
BstAPI GCANNNNNTGC 1 cut(s) 436
BstC8I GCNNGC 2 cut(s) 222, 810
BstDEI CTNAG 3 cut(s) 290, 738, 824
BstEII GGTNACC 1 cut(s) 278
BstF5I GGATG 3 cut(s) 167, 983, 1063
BstHHI GCGC 1 cut(s) 296
BstKTI GATC 8 cut(s) 84, 376, 455, 556, 671, 681, 835, 1129
BstMAI GTCTC 3 cut(s) 129, 197, 710
BstMBI GATC 8 cut(s) 81, 373, 452, 553, 668, 678, 832, 1126
BstMCI CGRYCG 1 cut(s) 225
BstMWI GCNNNNNNNGC 4 cut(s) 55, 341, 350, 436
BstNSI RCATGY 1 cut(s) 1155
BstPAI GACNNNNGTC 1 cut(s) 543
BstPI GGTNACC 1 cut(s) 278
BstSFI CTRYAG 1 cut(s) 348
BstV1I GCAGC 4 cut(s) 362, 832, 1069, 1091
BstV2I GAAGAC 1 cut(s) 250
BstX2I RGATCY 4 cut(s) 373, 678, 832, 1126
BstYI RGATCY 4 cut(s) 373, 678, 832, 1126
BstZI CGGCCG 1 cut(s) 222
Bsu15I ATCGAT 1 cut(s) 255
BsuRI GGCC 4 cut(s) 224, 694, 767, 812
BsuTUI ATCGAT 1 cut(s) 255
BtsCI GGATG 3 cut(s) 167, 983, 1063
BtsI GCAGTG 2 cut(s) 345, 969
BtsIMutI CAGTG 3 cut(s) 345, 621, 969
Cac8I GCNNGC 2 cut(s) 222, 810
CaiI CAGNNNCTG 2 cut(s) 152, 436
CfoI GCGC 1 cut(s) 296
Cfr10I RCCGGY 1 cut(s) 220
Cfr13I GGNCC 1 cut(s) 692
ClaI ATCGAT 1 cut(s) 255
Csp6I GTAC 3 cut(s) 7, 511, 955
CviAII CATG 4 cut(s) 329, 580, 1026, 1152
CviQI GTAC 3 cut(s) 7, 511, 955
DdeI CTNAG 3 cut(s) 290, 738, 824
DpnI GATC 8 cut(s) 83, 375, 454, 555, 670, 680, 834, 1128
DpnII GATC 8 cut(s) 81, 373, 452, 553, 668, 678, 832, 1126
EaeI YGGCCR 1 cut(s) 222
EagI CGGCCG 1 cut(s) 222
Eam1104I CTCTTC 1 cut(s) 1140
EarI CTCTTC 1 cut(s) 1140
Ecl136II GAGCTC 1 cut(s) 919
EclXI CGGCCG 1 cut(s) 222
Eco130I CCWWGG 2 cut(s) 90, 501
Eco147I AGGCCT 1 cut(s) 767
Eco24I GRGCYC 1 cut(s) 921
Eco31I GGTCTC 1 cut(s) 197
Eco52I CGGCCG 1 cut(s) 222
Eco53kI GAGCTC 1 cut(s) 919
Eco57I CTGAAG 1 cut(s) 824
Eco88I CYCGRG 1 cut(s) 311
Eco91I GGTNACC 1 cut(s) 278
EcoICRI GAGCTC 1 cut(s) 919
EcoO65I GGTNACC 1 cut(s) 278
EcoT14I CCWWGG 2 cut(s) 90, 501
EcoT22I ATGCAT 1 cut(s) 604
EcoT38I GRGCYC 1 cut(s) 921
ErhI CCWWGG 2 cut(s) 90, 501
FaeI CATG 4 cut(s) 332, 583, 1029, 1155
FaqI GGGAC 2 cut(s) 328, 387
FatI CATG 4 cut(s) 328, 579, 1025, 1151
FauI CCCGC 1 cut(s) 382
FauNDI CATATG 1 cut(s) 216
Fnu4HI GCNGC 5 cut(s) 165, 351, 846, 1080, 1083
FokI GGATG 3 cut(s) 154, 990, 1070
FriOI GRGCYC 1 cut(s) 921
Fsp4HI GCNGC 5 cut(s) 165, 351, 846, 1080, 1083
FspBI CTAG 2 cut(s) 161, 1044
GlaI GCGC 1 cut(s) 295
GluI GCNGC 5 cut(s) 165, 351, 846, 1080, 1083
GsuI CTGGAG 1 cut(s) 1134
HaeIII GGCC 4 cut(s) 224, 694, 767, 812
HapII CCGG 3 cut(s) 221, 225, 945
HhaI GCGC 1 cut(s) 296
Hin1II CATG 4 cut(s) 332, 583, 1029, 1155
Hin6I GCGC 1 cut(s) 294
HinP1I GCGC 1 cut(s) 294
HindIII AAGCTT 1 cut(s) 522
HinfI GANTC 6 cut(s) 137, 231, 246, 271, 305, 366
HpaII CCGG 3 cut(s) 221, 225, 945
HphI GGTGA 1 cut(s) 272
Hpy166II GTNNAC 2 cut(s) 158, 1051
Hpy188I TCNGA 6 cut(s) 134, 148, 238, 365, 549, 1126
Hpy188III TCNNGA 6 cut(s) 302, 313, 450, 611, 643, 826
Hpy8I GTNNAC 2 cut(s) 158, 1051
HpyAV CCTTC 3 cut(s) 182, 415, 823
HpyCH4III ACNGT 2 cut(s) 107, 997
HpyF10VI GCNNNNNNNGC 4 cut(s) 55, 341, 350, 436
HpyF3I CTNAG 3 cut(s) 290, 738, 824
Hsp92II CATG 4 cut(s) 332, 583, 1029, 1155
HspAI GCGC 1 cut(s) 294
KroI GCCGGC 1 cut(s) 220
KroNI GCCGGC 1 cut(s) 222
Kzo9I GATC 8 cut(s) 81, 373, 452, 553, 668, 678, 832, 1126
LmnI GCTCC 6 cut(s) 44, 662, 924, 947, 983, 1115
Lsp1109I GCAGC 4 cut(s) 362, 832, 1069, 1091
LweI GCATC 3 cut(s) 176, 478, 611
MaeI CTAG 2 cut(s) 161, 1044
MaeIII GTNAC 3 cut(s) 278, 575, 1154
MalI GATC 8 cut(s) 83, 375, 454, 555, 670, 680, 834, 1128
MboI GATC 8 cut(s) 81, 373, 452, 553, 668, 678, 832, 1126
MboII GAAGA 5 cut(s) 115, 240, 255, 827, 1127
MflI RGATCY 4 cut(s) 373, 678, 832, 1126
MhlI GDGCHC 1 cut(s) 921
MluCI AATT 6 cut(s) 142, 319, 337, 990, 1088, 1104
MlyI GAGTC 4 cut(s) 131, 240, 280, 360
MnlI CCTC 5 cut(s) 499, 556, 1048, 1069, 1143
Mph1103I ATGCAT 1 cut(s) 604
MroNI GCCGGC 1 cut(s) 220
MseI TTAA 2 cut(s) 494, 792
MspA1I CMGCKG 3 cut(s) 389, 737, 1082
MspI CCGG 3 cut(s) 221, 225, 945
Mva1269I GAATGC 1 cut(s) 848
MwoI GCNNNNNNNGC 4 cut(s) 55, 341, 350, 436
NaeI GCCGGC 1 cut(s) 222
NdeI CATATG 1 cut(s) 216
NdeII GATC 8 cut(s) 81, 373, 452, 553, 668, 678, 832, 1126
NgoMIV GCCGGC 1 cut(s) 220
NlaIII CATG 4 cut(s) 332, 583, 1029, 1155
NlaIV GGNNCC 4 cut(s) 680, 693, 897, 943
NmuCI GTSAC 2 cut(s) 278, 1154
NsiI ATGCAT 1 cut(s) 604
NspI RCATGY 1 cut(s) 1155
PceI AGGCCT 1 cut(s) 767
PctI GAATGC 1 cut(s) 848
PdiI GCCGGC 1 cut(s) 222
PfeI GAWTC 2 cut(s) 231, 305
PflMI CCANNNNNTGG 2 cut(s) 197, 392
PkrI GCNGC 5 cut(s) 166, 352, 847, 1081, 1084
PleI GAGTC 4 cut(s) 131, 240, 279, 360
PpsI GAGTC 4 cut(s) 131, 240, 279, 360
PshAI GACNNNNGTC 1 cut(s) 543
Psp124BI GAGCTC 1 cut(s) 921
PspEI GGTNACC 1 cut(s) 278
PspN4I GGNNCC 4 cut(s) 680, 693, 897, 943
PspPI GGNCC 1 cut(s) 692
PstI CTGCAG 1 cut(s) 352
PstNI CAGNNNCTG 2 cut(s) 152, 436
PsuI RGATCY 4 cut(s) 373, 678, 832, 1126
PvuII CAGCTG 2 cut(s) 737, 1082
RsaI GTAC 3 cut(s) 8, 512, 956
RsaNI GTAC 3 cut(s) 7, 511, 955
SacI GAGCTC 1 cut(s) 921
SaqAI TTAA 2 cut(s) 494, 792
SatI GCNGC 5 cut(s) 165, 351, 846, 1080, 1083
Sau3AI GATC 8 cut(s) 81, 373, 452, 553, 668, 678, 832, 1126
Sau96I GGNCC 1 cut(s) 692
ScaI AGTACT 1 cut(s) 512
SchI GAGTC 4 cut(s) 131, 240, 280, 360
SduI GDGCHC 1 cut(s) 921
SfaNI GCATC 3 cut(s) 176, 478, 611
SfcI CTRYAG 1 cut(s) 348
Sse9I AATT 6 cut(s) 142, 319, 337, 990, 1088, 1104
SseBI AGGCCT 1 cut(s) 767
SsiI CCGC 2 cut(s) 165, 389
SspI AATATT 1 cut(s) 483
SspMI CTAG 2 cut(s) 161, 1044
SstI GAGCTC 1 cut(s) 921
StuI AGGCCT 1 cut(s) 767
StyI CCWWGG 2 cut(s) 90, 501
TaaI ACNGT 2 cut(s) 107, 997
TaqI TCGA 2 cut(s) 255, 537
TasI AATT 6 cut(s) 142, 319, 337, 990, 1088, 1104
TatI WGTACW 2 cut(s) 510, 954
TauI GCSGC 1 cut(s) 167
TfiI GAWTC 2 cut(s) 231, 305
Tru1I TTAA 2 cut(s) 494, 792
Tru9I TTAA 2 cut(s) 494, 792
TscAI CASTG 3 cut(s) 352, 628, 976
TseFI GTSAC 2 cut(s) 278, 1154
TseI GCWGC 4 cut(s) 350, 845, 1079, 1082
Tsp45I GTSAC 2 cut(s) 278, 1154
TspDTI ATGAA 3 cut(s) 789, 1014, 1055
TspRI CASTG 3 cut(s) 352, 628, 976
Van91I CCANNNNNTGG 2 cut(s) 197, 392
XapI RAATTY 5 cut(s) 142, 319, 337, 1088, 1104
XceI RCATGY 1 cut(s) 1155
XcmI CCANNNNNNNNNTGG 1 cut(s) 1016
XspI CTAG 2 cut(s) 161, 1044
ZrmI AGTACT 1 cut(s) 512
Zsp2I ATGCAT 1 cut(s) 604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.