Prupe.1G497900_v2.0.a1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000978
Physical Location & Seq
Forward (+)
41257376 .. 41259923
2548 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G497900.1

Sequence Viewer

Length: 1170 bp
ATGGAGTCTTCTAGAATTCCTATCAAAGCTATCGTTTCTCTATTTTGCTCTTATATGCTTTTGTCGCACATCACCACTTTGGGCCTTACCTTGGCTTTGGAAGACTGTGAGTTTCCAGCCATCTTTAACTTTGGAGATTCAAATTCAGATACCGGTGGATTGGCTGCATCCCTTTCGCCACCACCACCACCTTATGGGGAGACCTATTTTCATATGCCGGTTGGAAGATTCTCAGATGGCAGGCTCATAATCGATTTTCTTGCAAAGAGTCTTGGTCACCCCTTTCTAAGCGCATATCTGGATTCTCTTGGGAGCAACTTCTCGTATGGTGCAAATTTTGCCACTGCAAATTCCACCATCAGATTGCCAAATGTCGTTCAACCAGCTGCTGGTGGATTTAGTCCCTTCTACCTCAATATTCAGTACATGCAGTTCATGCAACTAAAATCCAGATCACAACTCATTAGGCATCGAGGGGGAATATTTGAAAATTTGATGCCCAAGGAGGAGTATTTCTCGAAAGCTTTATACACATTTGACATTGGCCAGAATGACCTAGCAGAAGGCTTCATTGGTAACTTGACTGTTCAAGAAGTCAATGCATCGGTTCCTAATATAATCAGTGGGTTCTCGGCAAATATTAAGAAAATATATAATTTGGGAGCGAGATCATTTTGGATCCACAACACTGGGCCAATTGGCTGTCTCGCTTACATCTTAGCCAATTTCCCAGCTCAAAAGGATGAGGCTGGCTGTGCAAAGTCATATAATGAAGTAGCTCAGCATTTTAACCACAAGCTGAAGGAAGCCACAGTTCAACTTAGGAAAGATCTACCTTTGGCTGCAATTACTTATGTAGACGTCTATTCAGTCAAACACTTTCTTTTCAAGGAACCCCAAAAATATGGGTTTGAGCTCCCACTTATTGCTTGTTGTGGGTATGGTGGCAAGTACAACTATAACCGTAGTTCTGGATGTGGAGGAACAGCTAGAGTTAATGGAAGCCAACTTTTTGTTGGCTCATGCAAAGATCCTTCCGTTAGAGTGAATTGGGATGGCATTCATTATACTGAGGCAGCTGCCAAGTTTATTTCTGATAAAATTTCAACTGGAGCATTTTCTGATCCACCCCTTGCTTTGAAACAAGCATGTCACAAGAATTTGGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005488 GO:0006950 GO:0006952 GO:0008150 GO:0016787 GO:0016788 GO:0019863 GO:0019865 GO:0044877 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

390

Amino Acids

42.66

Weight (kDa)

8.26

Isoelectric Point (pI)

42.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 864
AccB7I CCANNNNNTGG 2 cut(s) 194, 389
AccI GTMKAC 1 cut(s) 858
AclWI GGATC 4 cut(s) 673, 686, 1025, 1118
AcoI YGGCCR 1 cut(s) 544
AcsI RAATTY 7 cut(s) 15, 142, 334, 349, 490, 1101, 1159
AcuI CTGAAG 1 cut(s) 821
AcyI GRCGYC 1 cut(s) 861
AfaI GTAC 2 cut(s) 425, 953
AfiI CCNNNNNNNGG 3 cut(s) 91, 194, 389
AgeI ACCGGT 1 cut(s) 152
AgsI TTSAA 8 cut(s) 141, 380, 488, 590, 818, 889, 1107, 1141
AjuI GAANNNNNNNTTGG 2 cut(s) 555, 587
AluBI AGCT 9 cut(s) 29, 386, 524, 734, 779, 799, 916, 989, 1079
AluI AGCT 9 cut(s) 29, 386, 524, 734, 779, 799, 916, 989, 1079
Alw21I GWGCWC 1 cut(s) 918
Alw26I GTCTC 2 cut(s) 194, 710
AlwI GGATC 4 cut(s) 673, 686, 1025, 1118
AlwNI CAGNNNCTG 1 cut(s) 389
AoxI GGCC 4 cut(s) 82, 544, 692, 1164
ApeKI GCWGC 5 cut(s) 164, 386, 842, 1076, 1079
ApoI RAATTY 7 cut(s) 15, 142, 334, 349, 490, 1101, 1159
AsiGI ACCGGT 1 cut(s) 152
AspLEI GCGC 1 cut(s) 293
AspS9I GGNCC 2 cut(s) 82, 692
AsuHPI GGTGA 2 cut(s) 64, 269
BalI TGGCCA 1 cut(s) 546
BamHI GGATCC 1 cut(s) 678
BanII GRGCYC 1 cut(s) 918
BbsI GAAGAC 1 cut(s) 108
Bbv12I GWGCWC 1 cut(s) 918
BbvI GCAGC 5 cut(s) 151, 373, 829, 1066, 1088
BccI CCATC 4 cut(s) 128, 230, 365, 1049
BcgI CGANNNNNNTGC 4 cut(s) 156, 190, 242, 276
BcoDI GTCTC 2 cut(s) 194, 710
BfaI CTAG 3 cut(s) 12, 557, 990
BglII AGATCT 1 cut(s) 829
BisI GCNGC 5 cut(s) 165, 387, 843, 1077, 1080
BlpI GCTNAGC 1 cut(s) 780
BlsI GCNGC 5 cut(s) 166, 388, 844, 1078, 1081
BmgT120I GGNCC 2 cut(s) 82, 692
BmiI GGNNCC 3 cut(s) 609, 680, 894
BmrI ACTGGG 1 cut(s) 699
BmsI GCATC 4 cut(s) 176, 478, 486, 611
BmuI ACTGGG 1 cut(s) 699
BpiI GAAGAC 1 cut(s) 108
BplI GAGNNNNNCTC 2 cut(s) 500, 532
BpmI CTGGAG 1 cut(s) 1131
Bpu1102I GCTNAGC 1 cut(s) 780
Bsa29I ATCGAT 1 cut(s) 252
BsaHI GRCGYC 1 cut(s) 861
BsaI GGTCTC 1 cut(s) 194
BsaJI CCNNGG 2 cut(s) 90, 501
BsaWI WCCGGW 1 cut(s) 152
Bsc4I CCNNNNNNNGG 3 cut(s) 91, 194, 389
Bse118I RCCGGY 2 cut(s) 152, 217
Bse1I ACTGG 2 cut(s) 694, 1114
BseCI ATCGAT 1 cut(s) 252
BseDI CCNNGG 2 cut(s) 90, 501
BseGI GGATG 4 cut(s) 167, 748, 980, 1060
BseLI CCNNNNNNNGG 3 cut(s) 91, 194, 389
BseMII CTCAG 3 cut(s) 246, 794, 1062
BseNI ACTGG 2 cut(s) 694, 1114
BseRI GAGGAG 1 cut(s) 521
BseXI GCAGC 5 cut(s) 151, 373, 829, 1066, 1088
BseYI CCCAGC 1 cut(s) 730
BshFI GGCC 4 cut(s) 84, 546, 694, 1166
BshTI ACCGGT 1 cut(s) 152
BshVI ATCGAT 1 cut(s) 252
BsiHKAI GWGCWC 1 cut(s) 918
BsiSI CCGG 2 cut(s) 153, 218
BslFI GGGAC 1 cut(s) 387
BslI CCNNNNNNNGG 3 cut(s) 91, 194, 389
BsmAI GTCTC 2 cut(s) 194, 710
BsmFI GGGAC 1 cut(s) 387
BsmI GAATGC 1 cut(s) 1059
BsnI GGCC 4 cut(s) 84, 546, 694, 1166
Bso31I GGTCTC 1 cut(s) 194
Bsp1286I GDGCHC 1 cut(s) 918
Bsp143I GATC 6 cut(s) 452, 668, 678, 829, 1030, 1123
Bsp1720I GCTNAGC 1 cut(s) 780
BspANI GGCC 4 cut(s) 84, 546, 694, 1166
BspCNI CTCAG 3 cut(s) 245, 793, 1063
BspDI ATCGAT 1 cut(s) 252
BspLI GGNNCC 3 cut(s) 609, 680, 894
BspPI GGATC 4 cut(s) 673, 686, 1025, 1118
BspTNI GGTCTC 1 cut(s) 194
BsrFI RCCGGY 2 cut(s) 152, 217
BsrI ACTGG 2 cut(s) 694, 1114
BssAI RCCGGY 2 cut(s) 152, 217
BssECI CCNNGG 2 cut(s) 90, 501
BssMI GATC 6 cut(s) 452, 668, 678, 829, 1030, 1123
BssNI GRCGYC 1 cut(s) 861
BssT1I CCWWGG 2 cut(s) 90, 501
Bst4CI ACNGT 4 cut(s) 107, 586, 814, 965
BstACI GRCGYC 1 cut(s) 861
BstAPI GCANNNNNTGC 2 cut(s) 338, 436
BstC8I GCNNGC 2 cut(s) 242, 751
BstDEI CTNAG 6 cut(s) 232, 287, 718, 780, 821, 1071
BstEII GGTNACC 1 cut(s) 275
BstF5I GGATG 4 cut(s) 167, 748, 980, 1060
BstHHI GCGC 1 cut(s) 293
BstKTI GATC 6 cut(s) 455, 671, 681, 832, 1033, 1126
BstMAI GTCTC 2 cut(s) 194, 710
BstMBI GATC 6 cut(s) 452, 668, 678, 829, 1030, 1123
BstMWI GCNNNNNNNGC 4 cut(s) 64, 338, 436, 755
BstNSI RCATGY 2 cut(s) 430, 1152
BstPI GGTNACC 1 cut(s) 275
BstV1I GCAGC 5 cut(s) 151, 373, 829, 1066, 1088
BstV2I GAAGAC 1 cut(s) 108
BstX2I RGATCY 3 cut(s) 678, 829, 1030
BstXI CCANNNNNNTGG 2 cut(s) 689, 905
BstYI RGATCY 3 cut(s) 678, 829, 1030
Bsu15I ATCGAT 1 cut(s) 252
BsuRI GGCC 4 cut(s) 84, 546, 694, 1166
BsuTUI ATCGAT 1 cut(s) 252
BtsCI GGATG 4 cut(s) 167, 748, 980, 1060
BtsI GCAGTG 1 cut(s) 342
BtsIMutI CAGTG 3 cut(s) 342, 628, 687
Cac8I GCNNGC 2 cut(s) 242, 751
CaiI CAGNNNCTG 1 cut(s) 389
CfoI GCGC 1 cut(s) 293
Cfr10I RCCGGY 2 cut(s) 152, 217
Cfr13I GGNCC 2 cut(s) 82, 692
ClaI ATCGAT 1 cut(s) 252
Csp6I GTAC 2 cut(s) 424, 952
CspAI ACCGGT 1 cut(s) 152
CviAII CATG 4 cut(s) 427, 436, 1023, 1149
CviQI GTAC 2 cut(s) 424, 952
DdeI CTNAG 6 cut(s) 232, 287, 718, 780, 821, 1071
DpnI GATC 6 cut(s) 454, 670, 680, 831, 1032, 1125
DpnII GATC 6 cut(s) 452, 668, 678, 829, 1030, 1123
EaeI YGGCCR 1 cut(s) 544
Ecl136II GAGCTC 1 cut(s) 916
Eco130I CCWWGG 2 cut(s) 90, 501
Eco24I GRGCYC 1 cut(s) 918
Eco31I GGTCTC 1 cut(s) 194
Eco53kI GAGCTC 1 cut(s) 916
Eco57I CTGAAG 1 cut(s) 821
Eco91I GGTNACC 1 cut(s) 275
EcoICRI GAGCTC 1 cut(s) 916
EcoO65I GGTNACC 1 cut(s) 275
EcoRI GAATTC 1 cut(s) 15
EcoT14I CCWWGG 2 cut(s) 90, 501
EcoT22I ATGCAT 1 cut(s) 604
EcoT38I GRGCYC 1 cut(s) 918
ErhI CCWWGG 2 cut(s) 90, 501
FaeI CATG 4 cut(s) 430, 439, 1026, 1152
FaqI GGGAC 1 cut(s) 387
FatI CATG 4 cut(s) 426, 435, 1022, 1148
FauNDI CATATG 1 cut(s) 213
FblI GTMKAC 1 cut(s) 858
Fnu4HI GCNGC 5 cut(s) 165, 387, 843, 1077, 1080
FokI GGATG 4 cut(s) 154, 755, 987, 1067
FriOI GRGCYC 1 cut(s) 918
Fsp4HI GCNGC 5 cut(s) 165, 387, 843, 1077, 1080
FspBI CTAG 3 cut(s) 12, 557, 990
GlaI GCGC 1 cut(s) 292
GluI GCNGC 5 cut(s) 165, 387, 843, 1077, 1080
GsaI CCCAGC 1 cut(s) 734
GsuI CTGGAG 1 cut(s) 1131
HaeIII GGCC 4 cut(s) 84, 546, 694, 1166
HapII CCGG 2 cut(s) 153, 218
HhaI GCGC 1 cut(s) 293
Hin1I GRCGYC 1 cut(s) 861
Hin1II CATG 4 cut(s) 430, 439, 1026, 1152
Hin6I GCGC 1 cut(s) 291
HinP1I GCGC 1 cut(s) 291
HindIII AAGCTT 1 cut(s) 522
HinfI GANTC 5 cut(s) 5, 137, 228, 268, 302
HpaII CCGG 2 cut(s) 153, 218
HphI GGTGA 2 cut(s) 64, 269
Hpy166II GTNNAC 1 cut(s) 859
Hpy188I TCNGA 5 cut(s) 148, 235, 362, 1096, 1123
Hpy188III TCNNGA 6 cut(s) 12, 299, 450, 517, 590, 972
Hpy8I GTNNAC 1 cut(s) 859
HpyAV CCTTC 4 cut(s) 415, 557, 796, 1044
HpyCH4III ACNGT 4 cut(s) 107, 586, 814, 965
HpyCH4IV ACGT 1 cut(s) 861
HpyF10VI GCNNNNNNNGC 4 cut(s) 64, 338, 436, 755
HpyF3I CTNAG 6 cut(s) 232, 287, 718, 780, 821, 1071
HpySE526I ACGT 1 cut(s) 861
Hsp92I GRCGYC 1 cut(s) 861
Hsp92II CATG 4 cut(s) 430, 439, 1026, 1152
HspAI GCGC 1 cut(s) 291
Kzo9I GATC 6 cut(s) 452, 668, 678, 829, 1030, 1123
LmnI GCTCC 4 cut(s) 312, 662, 921, 1112
Lsp1109I GCAGC 5 cut(s) 151, 373, 829, 1066, 1088
LweI GCATC 4 cut(s) 176, 478, 486, 611
MaeI CTAG 3 cut(s) 12, 557, 990
MaeII ACGT 1 cut(s) 861
MaeIII GTNAC 3 cut(s) 275, 575, 1151
MalI GATC 6 cut(s) 454, 670, 680, 831, 1032, 1125
MboI GATC 6 cut(s) 452, 668, 678, 829, 1030, 1123
MboII GAAGA 2 cut(s) 113, 237
MfeI CAATTG 1 cut(s) 696
MflI RGATCY 3 cut(s) 678, 829, 1030
MhlI GDGCHC 1 cut(s) 918
MlsI TGGCCA 1 cut(s) 546
MluNI TGGCCA 1 cut(s) 546
MlyI GAGTC 2 cut(s) 14, 277
MmeI TCCRAC 1 cut(s) 202
MnlI CCTC 6 cut(s) 422, 467, 499, 739, 974, 1066
Mox20I TGGCCA 1 cut(s) 546
Mph1103I ATGCAT 1 cut(s) 604
MscI TGGCCA 1 cut(s) 546
MseI TTAA 4 cut(s) 126, 642, 789, 996
Msp20I TGGCCA 1 cut(s) 546
MspA1I CMGCKG 2 cut(s) 386, 1079
MspI CCGG 2 cut(s) 153, 218
MunI CAATTG 1 cut(s) 696
Mva1269I GAATGC 1 cut(s) 1059
MwoI GCNNNNNNNGC 4 cut(s) 64, 338, 436, 755
NdeI CATATG 1 cut(s) 213
NdeII GATC 6 cut(s) 452, 668, 678, 829, 1030, 1123
NlaIII CATG 4 cut(s) 430, 439, 1026, 1152
NlaIV GGNNCC 3 cut(s) 609, 680, 894
NmeAIII GCCGAG 1 cut(s) 611
NmuCI GTSAC 2 cut(s) 275, 1151
NsiI ATGCAT 1 cut(s) 604
NspI RCATGY 2 cut(s) 430, 1152
PctI GAATGC 1 cut(s) 1059
PfeI GAWTC 3 cut(s) 137, 228, 302
PflMI CCANNNNNTGG 2 cut(s) 194, 389
PinAI ACCGGT 1 cut(s) 152
PkrI GCNGC 5 cut(s) 166, 388, 844, 1078, 1081
PleI GAGTC 2 cut(s) 13, 276
PpsI GAGTC 2 cut(s) 13, 276
Psp124BI GAGCTC 1 cut(s) 918
PspEI GGTNACC 1 cut(s) 275
PspFI CCCAGC 1 cut(s) 730
PspN4I GGNNCC 3 cut(s) 609, 680, 894
PspPI GGNCC 2 cut(s) 82, 692
PsrI GAACNNNNNNTAC 2 cut(s) 416, 448
PstNI CAGNNNCTG 1 cut(s) 389
PsuI RGATCY 3 cut(s) 678, 829, 1030
PvuII CAGCTG 2 cut(s) 386, 1079
RsaI GTAC 2 cut(s) 425, 953
RsaNI GTAC 2 cut(s) 424, 952
SacI GAGCTC 1 cut(s) 918
SaqAI TTAA 4 cut(s) 126, 642, 789, 996
SatI GCNGC 5 cut(s) 165, 387, 843, 1077, 1080
Sau3AI GATC 6 cut(s) 452, 668, 678, 829, 1030, 1123
Sau96I GGNCC 2 cut(s) 82, 692
SchI GAGTC 2 cut(s) 14, 277
SduI GDGCHC 1 cut(s) 918
SfaNI GCATC 4 cut(s) 176, 478, 486, 611
SspI AATATT 3 cut(s) 418, 483, 640
SspMI CTAG 3 cut(s) 12, 557, 990
SstI GAGCTC 1 cut(s) 918
StyI CCWWGG 2 cut(s) 90, 501
TaaI ACNGT 4 cut(s) 107, 586, 814, 965
TaiI ACGT 1 cut(s) 864
TaqI TCGA 3 cut(s) 252, 472, 518
TatI WGTACW 2 cut(s) 423, 951
TfiI GAWTC 3 cut(s) 137, 228, 302
Tru1I TTAA 4 cut(s) 126, 642, 789, 996
Tru9I TTAA 4 cut(s) 126, 642, 789, 996
TscAI CASTG 3 cut(s) 349, 628, 694
TseFI GTSAC 2 cut(s) 275, 1151
TseI GCWGC 5 cut(s) 164, 386, 842, 1076, 1079
Tsp45I GTSAC 2 cut(s) 275, 1151
TspDTI ATGAA 5 cut(s) 200, 424, 559, 786, 1052
TspGWI ACGGA 1 cut(s) 1027
TspRI CASTG 3 cut(s) 349, 628, 694
Van91I CCANNNNNTGG 2 cut(s) 194, 389
XapI RAATTY 7 cut(s) 15, 142, 334, 349, 490, 1101, 1159
XbaI TCTAGA 1 cut(s) 11
XceI RCATGY 2 cut(s) 430, 1152
XcmI CCANNNNNNNNNTGG 1 cut(s) 1013
XmiI GTMKAC 1 cut(s) 858
XspI CTAG 3 cut(s) 12, 557, 990
ZraI GACGTC 1 cut(s) 862
Zsp2I ATGCAT 1 cut(s) 604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.