Prupe.1G497500_v2.0.a1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000978
Physical Location & Seq
Forward (+)
41210335 .. 41212514
2180 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G497500.1

Sequence Viewer

Length: 1176 bp
ATGGAGTCTCCCAGAATTAATATCAAAGTTATCATTTCTCTATTTTGCTCTTATATGCTTTCGTCACATTATATCACCGCTTTGAGGCCTGCCTTGGCTATAAAAGAGTGTGAATTTCCAGCAATCTTTAACTTCGGAGACTCAAATTCAGATACTGGTGGATTGGCTGCATCCATTTATCCATCACCACCACCTTATGGAGAAACCTATTTTCATATTCCGGCTGGAAGAATCTCGGATGGCAGGCTCATAATCGATTTTCTTGCAAAGAATCTTGGTCAACCCTTTCTAAGTGCATATCTGGATTCACTTGGGGCCAACTTCTCGCACGGTGCAAATTTTGCCACTGGATCTTCGACCATCAGATTGCCAAATCGCATTTTACCAGCTCCTGGTGGATATAGTCCCTTCACCCTCACTATTCAATACTCGCAGTTCTTGCAACTAAAAAACAGATCACAACTCATTAGGGATCGAGGGGGAATATTTGCAAGTTTGATGCCCAAGGAGGAGTATTTTTCCAAAGCCTTGTACACATTTGACATTGGCCAGAATGACCTTGGAGAAGGGTTCTTTGCTAACTTGACTATTCAACAAGTCAATGCATCTATTCCTGATATAATCAGTAGCTTCTCAGCAAATATTAAGAAAATATATGATTTGGGAGGGAGATCATTTTGGATCCACAACACTGGGCCAATTGGCTGTCTCCCTTACATCTTAGCCAATTTCCCATCTCAAAAGGATGAGGCTGGCTGTGCAAAGTCGCACAATGAAGTAGCTCAGCAATTTAACCACAACCTGAAGGAGGCTACAGTTCAACTTAGGAAAGATCTACGTTTGGCTGCAATTACTTATGTAGACGTCTATTCAGTCAAATACTCTCTTTACAAGGAACCTAAAAAATATGGGTTTGAGCTCCCACTTGTTGGTTGTTGTGGCTCTGGTGGCAAGTACAACTTTAACGGTAGAACTCTTGATTGTGGAAGAACAGTTACAGTTAATGGAAGACAAATATTTGCTGGCTCATGCAAACGTCCTTATGTTAAAGTGAACTGGGATGGGATTCATTATACTGAGGCAGCAGCCAAGATTGTTTTTGATAAAATTTCAACTGGAGCTTTTTCCGATCCACCCCTTCCTTTCAAACAAGCATGTCACAGGAGTTTGGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005488 GO:0006950 GO:0006952 GO:0008150 GO:0016787 GO:0016788 GO:0019863 GO:0019865 GO:0044877 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

392

Amino Acids

43.17

Weight (kDa)

8.89

Isoelectric Point (pI)

45.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 867
AccB7I CCANNNNNTGG 3 cut(s) 197, 392, 929
AccI GTMKAC 1 cut(s) 861
AciI CCGC 1 cut(s) 78
AclWI GGATC 5 cut(s) 358, 480, 676, 689, 1124
AcoI YGGCCR 1 cut(s) 547
AcsI RAATTY 4 cut(s) 113, 145, 337, 1107
AcuI CTGAAG 1 cut(s) 824
AcyI GRCGYC 1 cut(s) 864
AfaI GTAC 2 cut(s) 533, 956
AfiI CCNNNNNNNGG 5 cut(s) 84, 197, 392, 808, 929
AgsI TTSAA 5 cut(s) 425, 593, 821, 1113, 1147
AjnI CCWGG 1 cut(s) 391
AluBI AGCT 5 cut(s) 389, 630, 782, 919, 1121
AluI AGCT 5 cut(s) 389, 630, 782, 919, 1121
Alw21I GWGCWC 1 cut(s) 921
Alw26I GTCTC 3 cut(s) 12, 132, 713
AlwI GGATC 5 cut(s) 358, 480, 676, 689, 1124
AlwNI CAGNNNCTG 2 cut(s) 155, 392
AoxI GGCC 4 cut(s) 86, 315, 547, 695
ApeKI GCWGC 4 cut(s) 167, 845, 1082, 1085
ApoI RAATTY 4 cut(s) 113, 145, 337, 1107
AseI ATTAAT 1 cut(s) 18
AspS9I GGNCC 2 cut(s) 315, 695
AsuHPI GGTGA 3 cut(s) 67, 177, 403
BalI TGGCCA 1 cut(s) 549
BamHI GGATCC 1 cut(s) 681
BanII GRGCYC 1 cut(s) 921
BarI GAAGNNNNNNTAC 2 cut(s) 979, 1011
BbsI GAAGAC 1 cut(s) 1015
Bbv12I GWGCWC 1 cut(s) 921
BbvI GCAGC 4 cut(s) 154, 832, 1094, 1097
BccI CCATC 5 cut(s) 190, 233, 368, 742, 1055
BcgI CGANNNNNNTGC 2 cut(s) 245, 279
BciT130I CCWGG 1 cut(s) 393
BcoDI GTCTC 3 cut(s) 12, 132, 713
BfmI CTRYAG 1 cut(s) 813
BglII AGATCT 1 cut(s) 832
BisI GCNGC 4 cut(s) 168, 846, 1083, 1086
BlpI GCTNAGC 1 cut(s) 783
BlsI GCNGC 4 cut(s) 169, 847, 1084, 1087
Bme1390I CCNGG 1 cut(s) 393
BmgT120I GGNCC 2 cut(s) 315, 695
BmiI GGNNCC 3 cut(s) 316, 683, 897
BmrFI CCNGG 1 cut(s) 393
BmrI ACTGGG 2 cut(s) 702, 1066
BmsI GCATC 3 cut(s) 179, 489, 614
BmuI ACTGGG 2 cut(s) 702, 1066
BpiI GAAGAC 1 cut(s) 1015
BpmI CTGGAG 1 cut(s) 1137
Bpu1102I GCTNAGC 1 cut(s) 783
Bsa29I ATCGAT 1 cut(s) 255
BsaHI GRCGYC 1 cut(s) 864
BsaJI CCNNGG 3 cut(s) 93, 504, 559
Bsc4I CCNNNNNNNGG 5 cut(s) 84, 197, 392, 808, 929
Bse1I ACTGG 5 cut(s) 160, 352, 697, 1061, 1120
BseBI CCWGG 1 cut(s) 393
BseCI ATCGAT 1 cut(s) 255
BseDI CCNNGG 3 cut(s) 93, 504, 559
BseGI GGATG 4 cut(s) 170, 244, 751, 1066
BseLI CCNNNNNNNGG 5 cut(s) 84, 197, 392, 808, 929
BseMII CTCAG 3 cut(s) 648, 797, 1068
BseNI ACTGG 5 cut(s) 160, 352, 697, 1061, 1120
BseRI GAGGAG 1 cut(s) 524
BseXI GCAGC 4 cut(s) 154, 832, 1094, 1097
BshFI GGCC 4 cut(s) 88, 317, 549, 697
BshVI ATCGAT 1 cut(s) 255
BsiHKAI GWGCWC 1 cut(s) 921
BsiSI CCGG 1 cut(s) 221
BslFI GGGAC 1 cut(s) 390
BslI CCNNNNNNNGG 5 cut(s) 84, 197, 392, 808, 929
BsmAI GTCTC 3 cut(s) 12, 132, 713
BsmFI GGGAC 1 cut(s) 390
BsnI GGCC 4 cut(s) 88, 317, 549, 697
Bsp1286I GDGCHC 1 cut(s) 921
Bsp1407I TGTACA 1 cut(s) 531
Bsp143I GATC 7 cut(s) 350, 455, 472, 671, 681, 832, 1129
Bsp1720I GCTNAGC 1 cut(s) 783
BspACI CCGC 1 cut(s) 78
BspANI GGCC 4 cut(s) 88, 317, 549, 697
BspCNI CTCAG 3 cut(s) 647, 796, 1069
BspDI ATCGAT 1 cut(s) 255
BspLI GGNNCC 3 cut(s) 316, 683, 897
BspPI GGATC 5 cut(s) 358, 480, 676, 689, 1124
BsrGI TGTACA 1 cut(s) 531
BsrI ACTGG 5 cut(s) 160, 352, 697, 1061, 1120
BssECI CCNNGG 3 cut(s) 93, 504, 559
BssMI GATC 7 cut(s) 350, 455, 472, 671, 681, 832, 1129
BssNI GRCGYC 1 cut(s) 864
BssT1I CCWWGG 3 cut(s) 93, 504, 559
Bst2UI CCWGG 1 cut(s) 393
Bst4CI ACNGT 5 cut(s) 332, 817, 968, 994, 1000
BstACI GRCGYC 1 cut(s) 864
BstAPI GCANNNNNTGC 2 cut(s) 341, 439
BstAUI TGTACA 1 cut(s) 531
BstC8I GCNNGC 4 cut(s) 90, 245, 754, 1024
BstDEI CTNAG 6 cut(s) 290, 634, 721, 783, 824, 1077
BstENI CCTNNNNNAGG 1 cut(s) 806
BstF5I GGATG 4 cut(s) 170, 244, 751, 1066
BstKTI GATC 7 cut(s) 353, 458, 475, 674, 684, 835, 1132
BstMAI GTCTC 3 cut(s) 12, 132, 713
BstMBI GATC 7 cut(s) 350, 455, 472, 671, 681, 832, 1129
BstMWI GCNNNNNNNGC 4 cut(s) 341, 439, 758, 948
BstNI CCWGG 1 cut(s) 393
BstNSI RCATGY 1 cut(s) 1158
BstSCI CCNGG 1 cut(s) 391
BstSFI CTRYAG 1 cut(s) 813
BstV1I GCAGC 4 cut(s) 154, 832, 1094, 1097
BstV2I GAAGAC 1 cut(s) 1015
BstX2I RGATCY 3 cut(s) 350, 681, 832
BstXI CCANNNNNNTGG 1 cut(s) 692
BstYI RGATCY 3 cut(s) 350, 681, 832
Bsu15I ATCGAT 1 cut(s) 255
BsuRI GGCC 4 cut(s) 88, 317, 549, 697
BsuTUI ATCGAT 1 cut(s) 255
BtsCI GGATG 4 cut(s) 170, 244, 751, 1066
BtsIMutI CAGTG 2 cut(s) 345, 690
Cac8I GCNNGC 4 cut(s) 90, 245, 754, 1024
CaiI CAGNNNCTG 2 cut(s) 155, 392
Cfr13I GGNCC 2 cut(s) 315, 695
ClaI ATCGAT 1 cut(s) 255
Csp6I GTAC 2 cut(s) 532, 955
CviAII CATG 2 cut(s) 1029, 1155
CviQI GTAC 2 cut(s) 532, 955
DdeI CTNAG 6 cut(s) 290, 634, 721, 783, 824, 1077
DpnI GATC 7 cut(s) 352, 457, 474, 673, 683, 834, 1131
DpnII GATC 7 cut(s) 350, 455, 472, 671, 681, 832, 1129
EaeI YGGCCR 1 cut(s) 547
Ecl136II GAGCTC 1 cut(s) 919
Eco130I CCWWGG 3 cut(s) 93, 504, 559
Eco147I AGGCCT 1 cut(s) 88
Eco24I GRGCYC 1 cut(s) 921
Eco53kI GAGCTC 1 cut(s) 919
Eco57I CTGAAG 1 cut(s) 824
EcoICRI GAGCTC 1 cut(s) 919
EcoNI CCTNNNNNAGG 1 cut(s) 806
EcoRII CCWGG 1 cut(s) 391
EcoT14I CCWWGG 3 cut(s) 93, 504, 559
EcoT22I ATGCAT 1 cut(s) 607
EcoT38I GRGCYC 1 cut(s) 921
ErhI CCWWGG 3 cut(s) 93, 504, 559
FaeI CATG 2 cut(s) 1032, 1158
FalI AAGNNNNNCTT 2 cut(s) 944, 976
FaqI GGGAC 1 cut(s) 390
FatI CATG 2 cut(s) 1028, 1154
FblI GTMKAC 1 cut(s) 861
Fnu4HI GCNGC 4 cut(s) 168, 846, 1083, 1086
FokI GGATG 4 cut(s) 157, 251, 758, 1073
FriOI GRGCYC 1 cut(s) 921
Fsp4HI GCNGC 4 cut(s) 168, 846, 1083, 1086
GluI GCNGC 4 cut(s) 168, 846, 1083, 1086
GsuI CTGGAG 1 cut(s) 1137
HaeIII GGCC 4 cut(s) 88, 317, 549, 697
HapII CCGG 1 cut(s) 221
Hin1I GRCGYC 1 cut(s) 864
Hin1II CATG 2 cut(s) 1032, 1158
HincII GTYRAC 1 cut(s) 281
HindII GTYRAC 1 cut(s) 281
HinfI GANTC 6 cut(s) 5, 140, 231, 271, 305, 1066
HpaII CCGG 1 cut(s) 221
HphI GGTGA 3 cut(s) 67, 177, 403
Hpy166II GTNNAC 4 cut(s) 281, 534, 862, 1054
Hpy188I TCNGA 5 cut(s) 137, 151, 238, 365, 1129
Hpy188III TCNNGA 3 cut(s) 302, 614, 977
Hpy8I GTNNAC 4 cut(s) 281, 534, 862, 1054
HpyAV CCTTC 4 cut(s) 418, 560, 799, 1148
HpyCH4III ACNGT 5 cut(s) 332, 817, 968, 994, 1000
HpyCH4IV ACGT 3 cut(s) 838, 864, 1036
HpyF10VI GCNNNNNNNGC 4 cut(s) 341, 439, 758, 948
HpyF3I CTNAG 6 cut(s) 290, 634, 721, 783, 824, 1077
HpySE526I ACGT 3 cut(s) 838, 864, 1036
Hsp92I GRCGYC 1 cut(s) 864
Hsp92II CATG 2 cut(s) 1032, 1158
Kzo9I GATC 7 cut(s) 350, 455, 472, 671, 681, 832, 1129
LmnI GCTCC 3 cut(s) 394, 924, 1118
Lsp1109I GCAGC 4 cut(s) 154, 832, 1094, 1097
LweI GCATC 3 cut(s) 179, 489, 614
MaeII ACGT 3 cut(s) 838, 864, 1036
MaeIII GTNAC 3 cut(s) 63, 994, 1157
MalI GATC 7 cut(s) 352, 457, 474, 673, 683, 834, 1131
MboI GATC 7 cut(s) 350, 455, 472, 671, 681, 832, 1129
MboII GAAGA 4 cut(s) 240, 345, 999, 1020
MfeI CAATTG 1 cut(s) 699
MflI RGATCY 3 cut(s) 350, 681, 832
MhlI GDGCHC 1 cut(s) 921
MlsI TGGCCA 1 cut(s) 549
MluCI AATT 9 cut(s) 15, 113, 145, 337, 699, 727, 788, 849, 1107
MluNI TGGCCA 1 cut(s) 549
MlyI GAGTC 2 cut(s) 14, 134
MnlI CCTC 8 cut(s) 78, 425, 470, 502, 659, 742, 802, 1072
Mox20I TGGCCA 1 cut(s) 549
Mph1103I ATGCAT 1 cut(s) 607
MscI TGGCCA 1 cut(s) 549
MseI TTAA 7 cut(s) 18, 129, 645, 792, 963, 1002, 1047
Msp20I TGGCCA 1 cut(s) 549
MspI CCGG 1 cut(s) 221
MspR9I CCNGG 1 cut(s) 393
MunI CAATTG 1 cut(s) 699
MvaI CCWGG 1 cut(s) 393
MwoI GCNNNNNNNGC 4 cut(s) 341, 439, 758, 948
NdeII GATC 7 cut(s) 350, 455, 472, 671, 681, 832, 1129
NlaIII CATG 2 cut(s) 1032, 1158
NlaIV GGNNCC 3 cut(s) 316, 683, 897
NmuCI GTSAC 2 cut(s) 63, 1157
NsiI ATGCAT 1 cut(s) 607
NspI RCATGY 1 cut(s) 1158
PceI AGGCCT 1 cut(s) 88
PfeI GAWTC 4 cut(s) 231, 271, 305, 1066
PflMI CCANNNNNTGG 3 cut(s) 197, 392, 929
PkrI GCNGC 4 cut(s) 169, 847, 1084, 1087
PleI GAGTC 2 cut(s) 13, 134
PpsI GAGTC 2 cut(s) 13, 134
PshBI ATTAAT 1 cut(s) 18
Psp124BI GAGCTC 1 cut(s) 921
Psp6I CCWGG 1 cut(s) 391
PspGI CCWGG 1 cut(s) 391
PspN4I GGNNCC 3 cut(s) 316, 683, 897
PspPI GGNCC 2 cut(s) 315, 695
PstNI CAGNNNCTG 2 cut(s) 155, 392
PsuI RGATCY 3 cut(s) 350, 681, 832
RsaI GTAC 2 cut(s) 533, 956
RsaNI GTAC 2 cut(s) 532, 955
SacI GAGCTC 1 cut(s) 921
SaqAI TTAA 7 cut(s) 18, 129, 645, 792, 963, 1002, 1047
SatI GCNGC 4 cut(s) 168, 846, 1083, 1086
Sau3AI GATC 7 cut(s) 350, 455, 472, 671, 681, 832, 1129
Sau96I GGNCC 2 cut(s) 315, 695
SchI GAGTC 2 cut(s) 14, 134
ScrFI CCNGG 1 cut(s) 393
SduI GDGCHC 1 cut(s) 921
SfaNI GCATC 3 cut(s) 179, 489, 614
SfcI CTRYAG 1 cut(s) 813
Sse9I AATT 9 cut(s) 15, 113, 145, 337, 699, 727, 788, 849, 1107
SseBI AGGCCT 1 cut(s) 88
SsiI CCGC 1 cut(s) 78
SspI AATATT 3 cut(s) 486, 643, 1017
SstI GAGCTC 1 cut(s) 921
StuI AGGCCT 1 cut(s) 88
StyD4I CCNGG 1 cut(s) 391
StyI CCWWGG 3 cut(s) 93, 504, 559
TaaI ACNGT 5 cut(s) 332, 817, 968, 994, 1000
TaiI ACGT 3 cut(s) 841, 867, 1039
TaqI TCGA 3 cut(s) 255, 356, 475
TasI AATT 9 cut(s) 15, 113, 145, 337, 699, 727, 788, 849, 1107
TatI WGTACW 2 cut(s) 531, 954
TfiI GAWTC 4 cut(s) 231, 271, 305, 1066
Tru1I TTAA 7 cut(s) 18, 129, 645, 792, 963, 1002, 1047
Tru9I TTAA 7 cut(s) 18, 129, 645, 792, 963, 1002, 1047
TscAI CASTG 2 cut(s) 352, 697
TseFI GTSAC 2 cut(s) 63, 1157
TseI GCWGC 4 cut(s) 167, 845, 1082, 1085
Tsp45I GTSAC 2 cut(s) 63, 1157
TspDTI ATGAA 3 cut(s) 203, 789, 1058
TspRI CASTG 2 cut(s) 352, 697
Van91I CCANNNNNTGG 3 cut(s) 197, 392, 929
VspI ATTAAT 1 cut(s) 18
XagI CCTNNNNNAGG 1 cut(s) 806
XapI RAATTY 4 cut(s) 113, 145, 337, 1107
XceI RCATGY 1 cut(s) 1158
XcmI CCANNNNNNNNNTGG 1 cut(s) 557
XmiI GTMKAC 1 cut(s) 861
ZraI GACGTC 1 cut(s) 865
Zsp2I ATGCAT 1 cut(s) 607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.